CN101861910A - Method for removing anti-nutritional factors from palm kernel dregs by using fermentation process - Google Patents
Method for removing anti-nutritional factors from palm kernel dregs by using fermentation process Download PDFInfo
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Abstract
The invention relates to a method for removing anti-nutritional factors from palm kernel dregs by using the fermentation process, comprising the following steps: (1) carrying out primary bacteria culturing and secondary bacteria culturing at 25-45 DEG C for 12-48h by controlling the rotation speed at 120-200 r/min, wherein the bacteria are bacillus and yeast, and preparing bacillus solution and yeast solution from the bacillus and the yeast; (2) adding a certain amount of water to the raw material of ground palm kernel dregs, sterilizing the ground palm kernel dregs at 121 DEG C for 15min, and fully mixing the ground palm kernel dregs, the bacillus solution and the yeast solution evenly; and (3) controlling the fermentation in such a way of fermenting the mixture at 30-60 DEG C and preserving the heat of the mixture for 5-15 days to obtain the fermented product. The invention solves the problems that the palm kernel dregs contain anti-nutritional factors and mannanase and have low yield and high cost. The method for removing the anti-nutritional factors from the palm kernel dregs by using the fermentation process has simple process, can ensure that no wastes can be generated and is suitable for the large-scale production.
Description
Technical field
The present invention relates to a kind of fermentation method that utilizes and prepare field of feed, be specifically related to a kind of degrade method of ANFs in the palm kernel dregs of rice of microorganism of utilizing, obtain a kind of product that can be used as feed or feed formula by this method, belong to the fermentation engineering field.
Background technology
The palm kernel dregs of rice are the byproducts after palm kernel passes through mechanical expression or solvent extraction oil expression, and its shape, color are similar to the vegetable seeds dregs of rice, slightly chocolate smell.The palm kernel dregs of rice are looked the different of its shelling degree and processing technology, and quality differs greatly.Its fiber content height, crude protein content is low, and lysine, methionine all lack (the main component composition sees Table 1) with tryptophan.
The main constituent of the table 1 palm kernel dregs of rice
The palm kernel dregs of rice are cheap, and no mouldiness and side effect are higher because of its crude fat content, it can be classified as energy feed in concrete the use, can substitute part corn or wheat bran according to a certain percentage, are specially adapted to the feed of ruminant such as ox, sheep, horse, deer etc.Use the palm kernel dregs of rice to substitute feeds such as part corn or dregs of beans, the most direct performance is: feed cost significantly reduces, but feeding effect is constant, thereby Feed Enterprise, herding economic benefit of enterprises are significantly improved, and the competitiveness in the colleague strengthens greatly.
Though the palm kernel dregs of rice in Europe of scale running, area such as Korea S widely uses; but because Chinese user relatively also is unfamiliar with the palm kernel dregs of rice; at present also less import is used; this illustrates that also the palm kernel dregs of rice have very big business development in China and are worth; so far, domestic to the palm kernel dregs of rice in feed application and study rarely seen report.
Have report to show, influence the palm kernel dregs of rice and be not only that as the reason that feed uses its crude fiber content is higher, main is because contain a large amount of ANFs in the palm kernel dregs of rice: beta-mannase.Beta-mannase comprises mannosan, galactomannans, Glucomannan etc.How to adopt degrade mannosan and make it to transfer to the nutriment that can be animal use of effective method, become problem demanding prompt solution of feedstuff industry.
At present, mainly come ANFs in passivation and the deactivation feed by physics, chemistry and biology approach, these methods can both reduce the content of ANFs to a certain extent.Compare physics and chemical method, the ANFs that adopts biological technique method to handle in the raw material more and more is subject to people's attention, and not only result of use is good, and cost is low, has become one of the research focus in this field.
β-1, (β-1 is that a class can hydrolysis contain β-1 4-D-mannanase) to the 4-D-mannase, and the 4-D-mannose is the restriction endonuclease of the polysaccharide (comprising mannosan, galactomannans, Glucomannan etc.) of main chain.This enzyme is of many uses, can decompose mannosan, and production can promote bifidobacterium growth, improve the compound sugar of gut flora structure; Can be used as toolenzyme and be used for the analysis of natural polysaccharide class formation; Can be in paper industry, with collaborative use of hemicellulose degraded enzyme such as beta-xylanase, can remove the hemicellulose in the paper pulp, improve papery; In textile industry, be used for the degraded of fiber hemicellulose, the excess dyestuff that can effectively remove textile and adhered to; Can also in feed industry, be used as enzyme additive, play the effect of eliminating ANFs.
The interpolation enzyme preparation can make the ANFs inactivation in the feed on the one hand, the substrate (ANFs or nutritional labeling) that the multiple needs of can degrading are on the other hand degraded, nutriment in the feed is degraded to small-molecule substance, help animal body and absorb, can improve the nutritive value of feed to greatest extent; Ferment by microorganism (bacterium and fungi), can make ANFs passivation or reduction thereby produce digestive enzyme.
Though having a extensive future of mannase, at present generally speaking, its low yield and expensive its range of application and the scale of having limited.
Summary of the invention
The present invention is exactly in order to address the above problem, overcome the palm kernel dregs of rice and use the problem that contains a large amount of ANFs as feed, and overcome mannase low yield and expensive problem, a kind of method of utilizing fermentation method to remove ANFs in the palm kernel dregs of rice is provided.It is simple to the invention provides a kind of technical process, and the microorganism solid fermentation that utilizes that does not produce a large amount of discarded objects is handled the method for the palm kernel dregs of rice, and this invention is applicable to large-scale production.
The present invention adopts the more single bacterium ferment effect of mixed fungus fermentation good, it is advantageous that the defective that can compensate self between a plurality of bacterial classifications mutually, carries out cooperative fermentation.
The present invention adopts solid state fermentation can simplify production technology, removes that liquid fermentation needs to dewater, collect and dry laborious procedures from, and the output height is arranged, and process is simple and easy, invest low, less cost of power, less to the loss of nutriment, prospect is very wide.
The technical problem that will solve required for the present invention can be achieved through the following technical solutions:
A kind of method of utilizing fermentation method to remove ANFs in the palm kernel dregs of rice adopts microorganism to mix degrade ANFs in the palm kernel dregs of rice of bacterium solid state fermentation, improves the method for the palm kernel dregs of rice as the nutritive value of feed, may further comprise the steps:
The first step, the preparation of zymophyte seed liquid:
Fermented bacterium is bacillus and saccharomycetic mixed bacterium, and bacillus and saccharomycete need carry out the first order seed cultivation and secondary seed spreads cultivation, and cultivation temperature is 25-45 ℃, and incubation time is 12-48h, and rotating speed is 120-200r/min, is prepared into zymophyte seed liquid;
(1) preparation of bacillus seed liquor
1) first order seed is cultivated: encircle thalline to the 250ml triangular flask that the 10ml seed culture medium is housed from the bacterial classification picking one of inclined-plane bacillus, place constant temperature speed governing shaking table to carry out thalline and cultivate, rotating speed is 170r/min, cultivates 24 hours for 30-40 ℃;
2) secondary seed spreads cultivation: draw 5ml first order seed nutrient solution in the 500ml triangular flask that the 100ml seed culture medium is housed, place constant temperature speed governing shaking table to carry out thalline and cultivate, rotating speed is 150-170r/min, cultivates 24 hours for 37 ℃, and is standby;
The preferred cultivation temperature of described bacillus is 37 ℃, and incubation time is 24h, and rotating speed is 170r/min;
Slant medium (g/L): beef extract 3g, peptone 10g, sodium chloride 5g, agar 20g, pH value 7.0-7.2,121 ℃ of sterilization 20min;
Seed culture medium (g/L): beef extract 3g, peptone 10g, sodium chloride 5g, pH value 7.0-7.2,121 ℃ of sterilization 20min;
Described bacillus is that bacillus subtilis (Bacillus subtilis), Bacillus circulans (Bacilluscirculans) and bacillus licheniformis (Bacillus licheniformis) are this laboratory preservation strain.
(2) preparation of saccharomycete seed liquid
1) first order seed is cultivated: saccharomycetic bacterial classification picking one ring thalline places constant temperature speed governing shaking table to carry out thalline and cultivates to the 250ml triangular flask that the 10ml seed culture medium is housed from the inclined-plane of preservation, and rotating speed is 150r/min, cultivates 24 hours for 30-40 ℃;
2) secondary seed spreads cultivation: draw 5ml first order seed nutrient solution in the 500ml triangular flask that the 100ml seed culture medium is housed, place constant temperature speed governing shaking table to carry out thalline and cultivate, rotating speed is 150-170r/min, cultivates 24 hours for 30 ℃, and is standby;
Described saccharomycetic cultivation temperature is 30 ℃, and incubation time is 24h, and rotating speed is 150r/min;
Slant medium (g/L): glucose 20g, peptone 10g, yeast extract 5g, agar 20g, 121 ℃ of sterilization 15min;
Seed culture medium (g/L): glucose 20g, peptone 10g, yeast extract 5g, 121 ℃ of sterilization 15min;
Described saccharomycete is that S. cervisiae (Saccharomyces cerevisiae) derives from bioengineering institute of Southern Yangtze University.
In second step, raw material mixes with bacillus seed liquor and saccharomycete seed liquid
Raw material adopts palm kernel, earlier the palm kernel dregs of rice is crushed to size for about 3-8mm, with the contact area of raising with bacterial classification, makes sweat more thorough; Add in the raw material behind a certain amount of water in 121 ℃ of sterilization 15min, insert bacillus seed liquor and saccharomycete seed liquid respectively, the access amount of bacillus seed liquor and saccharomycete seed liquid is the 5-20% of palm kernel dregs of rice quality, fully mixes, and the moisture that mixes the back material is controlled at 30-70%.
It is good for 5mm that the described palm kernel dregs of rice are crushed to size.
In the described step (2): the mass ratio of bacillus seed liquor and saccharomycete seed liquid is 1: 1.
The 3rd step, the control of fermentation process
The said mixture material placed under 30-60 ℃ the temperature and ferment, pH5-9 was incubated after 5-15 days, obtained tunning.Described mixed material is transferred pH6, and placing temperature is 40 ℃, and fermentation time was good for 10-15 days.
Pass through said method, adopt degrade mannosan in the palm kernel dregs of rice of microbial fermentation, obtain the higher mixture of water content, this product can be directly as the feed feeding animals, after also can carrying out drying, pulverizing to the product after the fermentation, obtain granular finished product and use as feed product.
After the palm kernel dregs of rice underwent microbial fermentation and handle, crude protein content brought up to 18% by original 14%, has improved 28.5%; The effect of the mannase that produces by growth of microorganism makes the content of reducing sugar in the palm kernel dregs of rice reach the 160mg/g palm kernel dregs of rice, and nutriment obviously improves.Applying of this product, the dependence of feed industry to feedstuffs such as dregs of beans, fish meal will be reduced greatly, having opened up new protein feed resources, will be to solve in the world that some countries produce huge economic benefit simultaneously to the effective way of the conventional feed worry problem that there is lack of raw materials.
The present invention utilizes the palm kernel dregs of rice to be solid state substrate, after fermented bacterium fully mixes, passes through microbial growth, beneficial microbe obtains a large amount of breedings, produce a large amount of mannases, the ANFs in the palm kernel dregs of rice of can degrading has accumulated some useful metabolites simultaneously.Mannase is a kind of of hemicellulase, can (comprise galactomannans by the hydrolysis mannosan, glucomannan and grape galactomannans) β-1,4 glycosidic bonds, can will extensively exist with the beans seed in mannosan be degraded to mannan-oligosaccharides, not only eliminated the anti-oxidant action of mannosan to nonruminant, the mannan-oligosaccharides of Sheng Chenging plays an important role in animal intestinal simultaneously, as promote the propagation of Bifidobacterium, effectively suppress pathogen and prevent diarrhoea; Reduce the generation of poisonous tunning and harmful bacteria, strengthen immunity of organisms etc.
Beneficial effect of the present invention:
1, contains a large amount of ANFs in the solution palm kernel dregs of rice, influence the problem that the palm kernel dregs of rice use as feed, use the palm kernel dregs of rice to substitute feeds such as part corn or dregs of beans, reduce feed cost.Alleviate the dependence of feed industry, opened up new protein feed resources, alleviate effective way, produce bigger economic benefit simultaneously the conventional feed worry problem that there is lack of raw materials to feedstuffs such as dregs of beans, fish meal.
2, the present invention adopts the more single bacterium ferment effect of mixed fungus fermentation good, it is advantageous that the defective that can compensate self between a plurality of bacterial classifications mutually, carries out cooperative fermentation.
3, the present invention adopts solid state fermentation can simplify production technology, removes that liquid fermentation needs to dewater, collect and dry laborious procedures from, and the output height is arranged, and process is simple and easy, invest low, less cost of power, less to the loss of nutriment, prospect is very wide.
4, do not produce a large amount of discarded objects in the production process of the present invention, free from environmental pollution.
Description of drawings
Further specify the present invention below in conjunction with the drawings and specific embodiments.
Fig. 1 is a brief operation flow chart of the present invention.
The specific embodiment
In order to make technological means of the present invention, creation characteristic, to reach purpose and effect is easy to understand,, further set forth the present invention below in conjunction with concrete diagram.
Embodiment 1
Brief operation flow chart of the present invention, as shown in Figure 1.
1, the preparation of fermented bacterium
(1) preparation of bacillus seed liquor
1) first order seed is cultivated: picking one ring thalline places constant temperature speed governing shaking table to carry out thalline and cultivates to the 250ml triangular flask that the 10ml seed culture medium is housed from the inclined-plane of the bacillus subtilis of preservation, and rotating speed is 170r/min, cultivates 24 hours for 37 ℃;
2) secondary seed spreads cultivation: draw 5ml first order seed nutrient solution in the 500ml triangular flask that the 100ml seed culture medium is housed, place constant temperature speed governing shaking table to carry out thalline and cultivate, rotating speed is 170r/min, cultivates 24 hours for 37 ℃, and is standby;
Slant medium (g/L): beef extract 3g, peptone 10g, sodium chloride 5g, agar 20g, pH value 7.0-7.2,121 ℃ of sterilization 20min;
Seed culture medium (g/L): beef extract 3g, peptone 10g, sodium chloride 5g, pH value 7.0-7.2,121 ℃ of sterilization 20min;
(2) preparation of saccharomycete seed liquid
1) first order seed is cultivated: encircle thalline to the 250ml triangular flask that the 10ml seed culture medium is housed from the saccharomyces cerevisiae inclined-plane picking one of preservation, place constant temperature speed governing shaking table to carry out thalline and cultivate, rotating speed is 150r/min, cultivates 24 hours for 30 ℃;
2) secondary seed spreads cultivation: draw 5ml first order seed nutrient solution in the 500ml triangular flask that the 100ml seed culture medium is housed, place constant temperature speed governing shaking table to carry out thalline and cultivate, rotating speed is 150r/min, cultivates 24 hours for 30 ℃, and is standby;
Slant medium (g/L): glucose 20g, peptone 10g, yeast extract 5g, agar 20g, 121 ℃ of sterilization 15min;
Seed culture medium (g/L): glucose 20g, peptone 10g, yeast extract 5g, 121 ℃ of sterilization 15min;
2, raw material and bacillus seed liquor and saccharomycete seed liquid mixes
Raw material adopts palm kernel, earlier the palm kernel dregs of rice is pulverized, and its size is about about 3mm, with the contact area of raising with bacterial classification, makes sweat more thorough, prepares palm kernel dregs of rice raw material 100g, adds the water of 30ml and stirs 121 ℃ of sterilization 15min.
Add bacillus secondary seed nutrient solution 10g and S. cervisiae secondary seed nutrient solution 10g respectively, fully mix, make mixed material.
3, the control of fermentation process
The said mixture material placed under 30 ℃ the temperature and ferment, pH9 was incubated after 5 days, obtained tunning.
Embodiment 2
As shown in Figure 1, raw material adopts palm kernel, earlier the palm kernel dregs of rice is pulverized, and its size is about about 8mm, prepares palm kernel dregs of rice raw material 100kg, adds the water of 30kg and stirs 121 ℃ of sterilization 15min; Add bacillus secondary seed nutrient solution and each 10kg of S. cervisiae secondary seed nutrient solution respectively, fully mix, make mixed material.
It is 5,40 ℃ of fermentations 15 days that the said mixture material is transferred pH, is drying to obtain finished product, and all the other are operated with embodiment 1.
Embodiment 3
As shown in Figure 1, raw material adopts palm kernel, earlier the palm kernel dregs of rice is pulverized, and its size is about about 5mm, prepares palm kernel dregs of rice raw material 100kg, adds the water of 10kg and stirs 121 ℃ of sterilization 15min; When secondary seed spread cultivation, the rotating speed of bacillus was 170r/min, and the rotating speed of S. cervisiae is 150r/min, added each 20kg of secondary seed nutrient solution of bacillus and S. cervisiae respectively, fully mixed; Make mixed material.
It is 6,40 ℃ of fermentations 10 days that the said mixture material is transferred pH, is drying to obtain finished product, and all the other are operated with embodiment 1.
Embodiment 4
As shown in Figure 1, raw material adopts palm kernel, earlier the palm kernel dregs of rice is pulverized, its size is about about 5mm, prepare palm kernel dregs of rice raw material 100kg, add the water of 50kg and stir 121 ℃ of sterilization 15min, when secondary seed spreads cultivation, the rotating speed of bacillus is 170r/min, and the rotating speed of S. cervisiae is 170r/min, adds each 10kg of secondary seed nutrient solution of bacillus 7kg and S. cervisiae respectively, fully mix, make mixed material.
It is 6.0,37 ℃ of fermentations 15 days that the said mixture material is transferred pH, is drying to obtain finished product, and all the other are operated with embodiment 1.
Embodiment 5
As shown in Figure 1, raw material adopts palm kernel, earlier the palm kernel dregs of rice is pulverized, its size is about about 5mm, prepare palm kernel dregs of rice raw material 100kg, add the water of 50kg and stir 121 ℃ of sterilization 15min, when secondary seed spreads cultivation, the rotating speed of bacillus is 160r/min, and the rotating speed of S. cervisiae is 160r/min, adds each 10kg of secondary seed nutrient solution of bacillus 7kg and S. cervisiae respectively, fully mix, make mixed material.
It is 6.5,35 ℃ of fermentations 15 days that the said mixture material is transferred pH, is drying to obtain finished product, and all the other are operated with embodiment 1.
Embodiment 6
As shown in Figure 1, raw material adopts palm kernel, earlier the palm kernel dregs of rice is pulverized, its size is about about 5mm, prepare palm kernel dregs of rice raw material 100kg, add the water of 70kg and stir 121 ℃ of sterilization 15min, when secondary seed spreads cultivation, the rotating speed of bacillus is 150r/min, and the rotating speed of S. cervisiae is 150r/min, adds each 1kg of secondary seed nutrient solution of bacillus 1kg and S. cervisiae respectively, fully mix, make mixed material.
It is 8,30 ℃ of fermentations 15 days that the said mixture material is transferred pH, is drying to obtain finished product, and all the other are operated with embodiment 1.
Embodiment 7
Raw material adopts palm kernel, earlier the palm kernel dregs of rice are pulverized, its size is about about 5mm, prepares palm kernel dregs of rice raw material 100kg, and the water of adding 30kg also stirs, 121 ℃ of sterilization 15min, when secondary seed spread cultivation, the rotating speed of bacillus was 150r/min, added the secondary seed nutrient solution 10kg of bacillus, fully mix, make mixed material.
It is 7,30 ℃ of fermentations 15 days that the said mixture material is transferred pH, is drying to obtain finished product, and all the other are operated with embodiment 1.
Embodiment 8
Raw material adopts palm kernel, earlier the palm kernel dregs of rice are pulverized, its size is about about 5mm, prepares palm kernel dregs of rice raw material 100kg, and the water of adding 30kg also stirs, 121 ℃ of sterilization 15min, when secondary seed spread cultivation, the rotating speed of S. cervisiae was 150r/min, added each 20kg of secondary seed nutrient solution of S. cervisiae, fully mix, make mixed material.
It is 7,30 ℃ of fermentations 15 days that the said mixture material is transferred pH, is drying to obtain finished product, and all the other are operated with embodiment 1.
Embodiment 9
The enzyme activity unit definition: under above-mentioned reaction condition, it is 1 enzyme activity unit (U) that per minute discharges the required enzyme amount of the reduced sugar that is equivalent to 1 μ molD-mannose by substrate.The mensuration of enzyme activity: get 0.4ml 0.5% (w/v) glucomannans (using pH7.0, the preparation of 0.01mol/L sodium phosphate buffer), add the enzyme liquid of 0.1ml debita spissitudo, 37 ℃ of water-bath 15min measure the reduced sugar that is produced with the DNS method.
The mensuration of content of reducing sugar accurately takes by weighing 2.5g palm kernel dregs of rice fermented sample, puts into the clean triangular flask of 150ml, uses 50ml80% ethanol, is incubated 60min in 70 ℃ of waters bath with thermostatic control, constantly shakes up therebetween; Get a certain amount of supernatant, adopt 3,5-dinitrosalicylic acid (DNS) method is measured the content of reduced sugar in the prepared fermentation palm kernel dregs of rice sample of embodiment 1-3, is respectively the 110mg/g palm kernel dregs of rice, the 130mg/g palm kernel dregs of rice and the 160mg/g palm kernel dregs of rice, the result of all the other embodiment sees table 2 for details.
Table 2 content of reducing sugar
Measure the crude protein content of this sample with Kjeldahl, accurately take by weighing the content of crude protein in the prepared fermentation palm kernel dregs of rice sample of sample embodiment 1-8, and the content of the palm kernel dregs of rice sample crude protein that do not ferment.
Take by weighing sample 0.50-2.00g → in 500ml Kai Shi bottle → add 10g anhydrous K
2SO
4→ add 0.5gCuSO
4→ add 20ml H
2SO
4→ in fume hood, heat with little fire earlier, after treating lather collapse, strengthen firepower, digestion is to the black grain of transparent nothing, bottle shaken make in bottle wall carbon granule vitriolization → continue digestion 30 minutes → be green state up to sample liquid, stop digestion, cool off → add 200ml water → connection distilling apparatus → make the raffinate in the KShi bottle of absorption liquid → in the KShi bottle, add fluctuation pearl number and 80ml50%NaOH → connect immediately nitrogen fixing ball → heating → extremely with boric acid to reduce to three/for the moment, taking-up water flushing → usefulness 0.1N HCl titration.
After its measurement result palm kernel dregs of rice underwent microbial fermentation and handle, crude protein content brought up to 18% by original 14%, has improved 28.5%, and the content of crude protein in the prepared fermentation palm kernel dregs of rice sample of embodiment 1-3 is referring to table 3.
Table 3 crude protein content
The present invention solves and contains a large amount of ANFs in the palm kernel dregs of rice, influence the problem that the palm kernel dregs of rice use as feed, use the palm kernel dregs of rice to substitute feeds such as part corn or dregs of beans, feed cost significantly reduces, but feeding effect is constant, thereby Feed Enterprise, herding economic benefit of enterprises are significantly improved, and the competitiveness in the colleague strengthens greatly.
The present invention adopts the more single bacterium ferment effect of mixed fungus fermentation good, it is advantageous that the defective that can compensate self between a plurality of bacterial classifications mutually, carries out cooperative fermentation.
The present invention adopts solid state fermentation can simplify production technology, removes that liquid fermentation needs to dewater, collect and dry laborious procedures from, and the output height is arranged, and process is simple and easy, invest low, less cost of power, less to the loss of nutriment, prospect is very wide.
Do not produce a large amount of discarded objects in the production process of the present invention, free from environmental pollution.
More than show and described basic principle of the present invention, principal character and advantage of the present invention.The technical staff of the industry should understand; the present invention is not restricted to the described embodiments; that describes in the foregoing description and the specification just illustrates principle of the present invention; the present invention also has various changes and modifications without departing from the spirit and scope of the present invention, and these changes and improvements all fall in the claimed scope of the invention.The claimed scope of the present invention is defined by appending claims and equivalent thereof.
Claims (10)
1. method of utilizing fermentation method to remove ANFs in the palm kernel dregs of rice, it is characterized in that, adopt microorganism to mix degrade ANFs in the palm kernel dregs of rice of bacterium solid state fermentation, improve the method for the palm kernel dregs of rice, may further comprise the steps as the nutritive value of feed:
The first step, the preparation of fermented bacterium: fermented bacterium is bacillus and saccharomycete, bacillus and saccharomycete need carry out the first order seed cultivation and secondary seed spreads cultivation, cultivation temperature is 25-45 ℃, incubation time is 12-48h, rotating speed is 120-200r/min, is prepared into bacillus seed liquor and saccharomycete seed liquid respectively;
Second step, raw material mixes with bacillus seed liquor and saccharomycete seed liquid: add in the palm kernel dregs of rice of pulverizing behind a certain amount of water in 121 ℃ of sterilization 15min, insert bacillus seed liquor and saccharomycete seed liquid then respectively, the access amount of bacillus seed liquor and saccharomycete seed liquid is for being the 5-20% of palm kernel dregs of rice quality, fully mix, the moisture that mixes the back material is controlled at 30-70%;
In the 3rd step, the control of fermentation process: described mixed material places under 30-60 ℃ the temperature and ferments, and is incubated after 5-15 days, obtains tunning.
2. method according to claim 1 is characterized in that: the preparation of bacillus seed liquor in the step (1),
1) first order seed is cultivated: encircle thalline to the 250ml triangular flask that the 10ml seed culture medium is housed from the bacterial classification picking one of inclined-plane bacillus, place constant temperature speed governing shaking table to carry out thalline and cultivate, rotating speed is 170r/min, cultivates 24 hours for 30-40 ℃;
2) secondary seed spreads cultivation: draw 5ml first order seed nutrient solution in the 500ml triangular flask that the 100ml seed culture medium is housed, place constant temperature speed governing shaking table to carry out thalline and cultivate, rotating speed is 150-170r/min, cultivates 24 hours for 37 ℃, and is standby;
3. method according to claim 2 is characterized in that: described slant medium is (g/L): beef extract 3g, peptone 10g, sodium chloride 5g, agar 20g, pH value 7.0-7.2,121 ℃ of sterilization 20min; Described seed culture medium (g/L): beef extract 3g, peptone 10g, sodium chloride 5g, pH value 7.0-7.2,121 ℃ of sterilization 20min;
4. method according to claim 1 is characterized in that: the preparation of saccharomycete seed liquid in the step (1),
1) first order seed is cultivated: saccharomycetic bacterial classification picking one ring thalline places constant temperature speed governing shaking table to carry out thalline and cultivates to the 250ml triangular flask that the 10ml seed culture medium is housed from the inclined-plane of preservation, and rotating speed is 150r/min, cultivates 24 hours for 30-40 ℃;
2) secondary seed spreads cultivation: draw 5ml first order seed nutrient solution in the 500ml triangular flask that the 100ml seed culture medium is housed, place constant temperature speed governing shaking table to carry out thalline and cultivate, rotating speed is 150-170r/min, cultivates 24 hours for 25-35 ℃, and is standby;
5. method according to claim 3 is characterized in that: slant medium (g/L): glucose 20g, peptone 10g, yeast extract 5g, agar 20g, 121 ℃ of sterilization 15min; Seed culture medium (g/L): glucose 20g, peptone 10g, yeast extract 5g, 121 ℃ of sterilization 15min.
6. method according to claim 2 is characterized in that, described bacillus cultivation temperature is 37 ℃, and incubation time is 24h, and rotating speed is 170r/min.
7. method according to claim 4 is characterized in that, fermented bacterium is a S. cervisiae, and the cultivation temperature of described S. cervisiae is 30 ℃, and incubation time is 24h, and rotating speed is 150r/min.
8. method according to claim 1 is characterized in that, the palm kernel dregs of rice described in the described step (2) are crushed to size for about 3-8mm, with the contact area of raising with bacterial classification, make sweat more thorough, and raw material is mixed with bacterial classification.
9. method according to claim 1 is characterized in that, mixed material is transferred pH5-9 in the described step (3), place under 30-40 ℃ the temperature to ferment 5-15 days,
10. method according to claim 9 is characterized in that, described mixed material is transferred pH6, and placing temperature is 40 ℃, and fermentation time is 10-15 days.
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| CN104872394A (en) * | 2015-06-05 | 2015-09-02 | 黄石市佳农饲料有限公司 | Palm-kernel meal with effect of removing antinutritional factors, preparation method and application thereof |
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| CN104172098A (en) * | 2014-07-09 | 2014-12-03 | 华南师范大学 | Technique for preparing soy sauce from palm pulp |
| CN104172098B (en) * | 2014-07-09 | 2015-07-22 | 华南师范大学 | Technique for preparing soy sauce from palm pulp |
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| CN106417897A (en) * | 2016-08-31 | 2017-02-22 | 上海源耀生物股份有限公司 | Palm meal fermentation feed and preparation method thereof |
| CN106417897B (en) * | 2016-08-31 | 2019-11-29 | 上海源耀生物股份有限公司 | A kind of palm kernel meal fermented feed and preparation method thereof |
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