CN101837047B - Method for preparing concentrated Yinhuang oral liquid - Google Patents
Method for preparing concentrated Yinhuang oral liquid Download PDFInfo
- Publication number
- CN101837047B CN101837047B CN2010101884868A CN201010188486A CN101837047B CN 101837047 B CN101837047 B CN 101837047B CN 2010101884868 A CN2010101884868 A CN 2010101884868A CN 201010188486 A CN201010188486 A CN 201010188486A CN 101837047 B CN101837047 B CN 101837047B
- Authority
- CN
- China
- Prior art keywords
- add
- weight
- radix scutellariae
- extract
- flos lonicerae
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Abstract
The invention relates to a method for preparing concentrated Yinhuang oral liquid, effectively increases the concentration of oral liquid and the content of active ingredients, and provides the method for preparing concentrated Yinhuang oral liquid which is convenient to carry, easy to take and high in efficacy. The invention adopts a technical scheme that 5 to 15 weight percent of honeysuckle flower extract and 85 to 95 weight percent of baikal skullcap root extract are taken as raw medicinal materials; and the method comprises the steps of preparing the honeysuckle flower extract and the baikal skullcap root extract first, adding water to dissolve the baikal skullcap root extract, using sodium hydroxide solution to regulate pH, filtering the obtained product, combining filtrate with the honeysuckle flower extract, using the sodium hydroxide solution to regulate pH, boiling and filtering the obtained product, adding simple syrup and water, mixing well, using the sodium hydroxide solution to regulate pH again, adding water to full dose, stirring uniformly, separating the obtained product through a high-speed centrifuge to obtain clear liquid, subjecting the clear liquid to ultra-filtration to obtain the filtrate, pouring, sealing and sterilizing the obtained product. The method has the advantages of reducing the amount of oral administration to half of the original taking amount, allowing the oral liquid to be easy to take, improving efficacy up to over 93 percent, reducing production cost and ensuring more taking-carrying convenience.
Description
One, technical field
The present invention relates to field of medicaments, particularly a kind of preparation method of concentrated Yinhuang oral liquid.
Two, background technology
YINHUANG KOUFUYE is a Chinese medicine preparation, has the effect of cleaning heat and expelling wind, liyan jiedu.Be widely used in affection due to external wind and heat, dry pharynx due to the exuberant lung-stomach heat, pharyngalgia, the pharyngeal tonsils enlargement, thirsty, heating, the acute and chronic tonsillitis, acute/chronic pharyngitis, the patient of symptoms such as upper respiratory tract infection, but this product processes is limit by technological level in one's early years, it is simple water extract-alcohol precipitation process for refining, impurity is many, the extraction and application rate is low, active constituent content is low, take weak effect, be unfavorable for that especially the child and the patient of the difficulty of taking medicine take, carry also inconvenient, curative effect is difficult to improve, the while raw material, the packaging material consumption is big, production cost is higher, has serious drawback on production technology.
Three, summary of the invention
At above-mentioned situation, for overcoming the prior art defective, the present invention's purpose is to adopt centrifugal, the membrance separation process for refining of Modern High-Speed, effectively improves oral liquid concentration and active constituent content, provides easy to carry, easily takes preparation method with the high a kind of concentrated Yinhuang oral liquid of curative effect.
The technical scheme that the present invention solves is, by by weight percentage: Flos Lonicerae extract 5-15%, Radix Scutellariae extract 85-95%, total amount 100% is done medicinal raw material, at first prepare Flos Lonicerae extract and Radix Scutellariae extract, Radix Scutellariae extract is dissolved in water, with sodium hydroxide solution adjust pH to 8.0, filter, filtrate and Flos Lonicerae extract merge, and get mixture, with sodium hydroxide solution adjust pH to 7.2, boiled 1 hour, filter, add the simple syrup of the 20%-30% of above-mentioned mixed liquor weight, add water to the 95%-98% (amount of the finished product oral liquid that finally is mixed with of full dose weight, be full dose, as follows), stir evenly reuse sodium hydroxide solution adjust pH to 7.2, add water to full dose, after stirring evenly, through high speed centrifuge separate clear liquid, it is that the ultrafilter membrane of 40000-80000 carries out ultrafiltration that clear liquid is selected molecular cut off for use, 10-45 ℃ of ultrafiltration temperature, operating pressure is 0.1-0.3Mpa, gets filtrate, embedding, sterilization, promptly.
The present invention and prior preparation method are relatively, its beneficial technical effects is: adopt the centrifugal and membrane separation technique of Modern High-Speed, removed invalid impurity, make medicine in unit volume, valid density to double, content of effective is the twice of original same dose, given full play to the effect of effective ingredient,, oral dose has been reduced because concentration and active constituent content improve, be half of original dose, be easy to take, curative effect improves, up to (former have only 88%) more than 93%, and raw material and packaging material have been saved greatly, reduce production cost, taken and carried more convenient, be particularly suitable for the child and the patient of the difficulty of taking medicine uses.
Four, the specific embodiment
Below in conjunction with practical situation the specific embodiment of the present invention is elaborated.
Preparation method of the present invention is: by by weight percentage: Flos Lonicerae extract 5-15%, and Radix Scutellariae extract 85-95%, total amount 100% is done medicinal raw material, makes according to the following steps:
(1), extracting honeysuckle, add mass concentration and be 15% alcohol reflux secondary, measured alcohol reflux 1 hour for 10 times that add for the first time Flos Lonicerae weight, measured alcohol reflux 1 hour for 8 times that add for the second time Flos Lonicerae weight, merge extracted twice liquid, being evaporated to 60 ℃, to survey relative densities (proportion, as follows) be 1.15～1.18 clear paste, adds ethanol and make and contain the alcohol amount and reach 65%, left standstill 24 hours, get supernatant, being evaporated to 60 ℃, to survey relative densities be 1.20～1.24 cream, and the 2-2.5 that adds water to Flos Lonicerae weight doubly, airtight, cold preservation was got supernatant more than 24 hours, promptly got Flos Lonicerae extract;
(2) get Radix Scutellariae, decoct with water secondary, 8 times of water gagings that add Radix Scutellariae weight for the first time decocted 1.5 hours, 6 times of water gagings that add for the second time Radix Scutellariae weight decocted 1.5 hours, filtered merging filtrate, filtrate is concentrated into 60 ℃, and to survey relative densities be 1.1～1.3 cream, with molar concentration is that the hydrochloric acid solution of 2mol/L is regulated pH value to 1.8～2.0,60 ℃ insulation 30 minutes, is cooled to 18-25 ℃, placed 12 hours, filter, remove supernatant, precipitate is washed till pH value to 4.0 with ethanol, 10 times of water gagings that add weight of precipitate, stirring, is 20% sodium hydroxide solution adjust pH to 7.0 with mass concentration, and the ethanol that adds after the dissolving with weight of precipitate equivalent stirs evenly, placed 12 hours, filter, filtrate is regulated pH value to 1.8～2.0,80 ℃ insulation 30 minutes with molar concentration for the 2mol/L hydrochloric acid solution, be cooled to 18-25 ℃, filter, remove supernatant, precipitate is washed till pH value to 4.0 with ethanol, drying under reduced pressure, pulverize, cross 80 orders or 100 mesh sieves, promptly get Radix Scutellariae extract;
(3) Radix Scutellariae extract adds 8 times of water gaging dissolvings of Radix Scutellariae extract weight, it with mass concentration 8% sodium hydroxide solution adjust pH to 8.0, filter, filtrate and Flos Lonicerae extract merge, get mixed liquor, with mass concentration is the pH value to 7.2 that 8% sodium hydroxide solution is transferred mixed liquor, boiled 1 hour, filter, the simple syrup that adds the 20%-30% of above-mentioned mixed liquor weight adds water to the 95%-98% of full dose weight, stirs evenly, with mass concentration is 8% sodium hydroxide solution adjust pH to 7.2, gets the mixed liquor of Flos Lonicerae extract and Radix Scutellariae extract;
(4) in the mixed liquor of Flos Lonicerae extract and Radix Scutellariae extract, add water to full dose, stir evenly after high speed centrifuge with 10000rpm/min-30000rpm/min separate clear liquid, it is that the ultrafilter membrane of 40000-80000 carries out ultrafiltration that clear liquid is selected molecular cut off for use, 10-45 ℃ of ultrafiltration temperature, operating pressure is 0.1-0.3Mpa, gets filtrate, embedding, sterilization, promptly.
Embodiment 1:
By by weight percentage: Flos Lonicerae extract 5%, Radix Scutellariae extract 95%, total amount 100% is done medicinal raw material, make according to the following steps:
(1), extracting honeysuckle, add mass concentration and be 15% alcohol reflux secondary, measured alcohol reflux 1 hour for 10 times that add for the first time Flos Lonicerae weight, measured alcohol reflux 1 hour for 8 times that add for the second time Flos Lonicerae weight, merge extracted twice liquid, being evaporated to 60 ℃, to survey relative densities be 1.15 clear paste, add ethanol and make and contain alcohol amount and reach 65%, left standstill 24 hours, get supernatant, being evaporated to 60 ℃, to survey relative densities be 1.20 cream, add water to 2 times of Flos Lonicerae weight, airtight, cold preservation is more than 24 hours, get supernatant, promptly get Flos Lonicerae extract;
(2) get Radix Scutellariae, decoct with water secondary, 8 times of water gagings that add Radix Scutellariae weight for the first time decocted 1.5 hours, 6 times of water gagings that add for the second time Radix Scutellariae weight decocted 1.5 hours, filtered merging filtrate, filtrate is concentrated into 60 ℃, and to survey relative densities be 1.1 cream, with molar concentration is the hydrochloric acid solution adjusting pH value to 1.8 of 2mol/L, and 60 ℃ are incubated 30 minutes, are cooled to 18 ℃, placed 12 hours, filter, remove supernatant, precipitate is washed till pH value to 4.0 with ethanol, 10 times of water gagings that add weight of precipitate, stirring, is 20% sodium hydroxide solution adjust pH to 7.0 with mass concentration, and the ethanol that adds after the dissolving with weight of precipitate equivalent stirs evenly, placed 12 hours, filter, filtrate is regulated pH value to 1.8 with molar concentration for the 2mol/L hydrochloric acid solution, and 80 ℃ are incubated 30 minutes, be cooled to 18 ℃, filter, remove supernatant, precipitate is washed till pH value to 4.0 with ethanol, drying under reduced pressure, pulverize, cross 80 mesh sieves, promptly get Radix Scutellariae extract;
(3) Radix Scutellariae extract adds 8 times of water gaging dissolvings of Radix Scutellariae extract weight, it with mass concentration 8% sodium hydroxide solution adjust pH to 8.0, filter, filtrate and Flos Lonicerae extract merge, get mixed liquor, with mass concentration is the pH value to 7.2 that 8% sodium hydroxide solution is transferred mixed liquor, boiled 1 hour, filter, add 20% simple syrup of above-mentioned mixed liquor weight, add water to 98% of full dose weight, stir evenly, with mass concentration is 8% sodium hydroxide solution adjust pH to 7.2, gets the mixed liquor of Flos Lonicerae extract and Radix Scutellariae extract;
(4) in the mixed liquor of Flos Lonicerae extract and Radix Scutellariae extract, add water to full dose, stir evenly after high speed centrifuge with 10000rpm/min separate clear liquid, it is that 40000 ultrafilter membrane carries out ultrafiltration that clear liquid is selected molecular cut off for use, 10 ℃ of ultrafiltration temperature, operating pressure is 0.3Mpa, gets filtrate, embedding, sterilization, promptly.
Embodiment 2:
By by weight percentage: Flos Lonicerae extract 15%, Radix Scutellariae extract 85%, total amount 100% is done medicinal raw material, make according to the following steps:
(1), extracting honeysuckle, add mass concentration and be 15% alcohol reflux secondary, measured alcohol reflux 1 hour for 10 times that add for the first time Flos Lonicerae weight, measured alcohol reflux 1 hour for 8 times that add for the second time Flos Lonicerae weight, merge extracted twice liquid, being evaporated to 60 ℃, to survey relative densities be 1.18 clear paste, add ethanol and make and contain alcohol amount and reach 65%, left standstill 24 hours, get supernatant, being evaporated to 60 ℃, to survey relative densities be 1.24 cream, add water to 2.5 times of Flos Lonicerae weight, airtight, cold preservation is more than 24 hours, get supernatant, promptly get Flos Lonicerae extract;
(2) get Radix Scutellariae, decoct with water secondary, 8 times of water gagings that add Radix Scutellariae weight for the first time decocted 1.5 hours, 6 times of water gagings that add for the second time Radix Scutellariae weight decocted 1.5 hours, filtered merging filtrate, filtrate is concentrated into 60 ℃, and to survey relative densities be 1.3 cream, with molar concentration is the hydrochloric acid solution adjusting pH value to 2.0 of 2mol/L, and 60 ℃ are incubated 30 minutes, are cooled to 25 ℃, placed 12 hours, filter, remove supernatant, precipitate is washed till pH value to 4.0 with ethanol, 10 times of water gagings that add weight of precipitate, stirring, is 20% sodium hydroxide solution adjust pH to 7.0 with mass concentration, and the ethanol that adds after the dissolving with weight of precipitate equivalent stirs evenly, placed 12 hours, filter, filtrate is regulated pH value to 2.0 with molar concentration for the 2mol/L hydrochloric acid solution, and 80 ℃ are incubated 30 minutes, be cooled to 25 ℃, filter, remove supernatant, precipitate is washed till pH value to 4.0 with ethanol, drying under reduced pressure, pulverize, cross 100 mesh sieves, promptly get Radix Scutellariae extract;
(3) Radix Scutellariae extract adds 8 times of water gaging dissolvings of Radix Scutellariae extract weight, it with mass concentration 8% sodium hydroxide solution adjust pH to 8.0, filter, filtrate and Flos Lonicerae extract merge, get mixed liquor, with mass concentration is the pH value to 7.2 that 8% sodium hydroxide solution is transferred mixed liquor, boiled 1 hour, filter, add 30% simple syrup of above-mentioned mixed liquor weight, add water to 95% of full dose weight, stir evenly, with mass concentration is 8% sodium hydroxide solution adjust pH to 7.2, gets the mixed liquor of Flos Lonicerae extract and Radix Scutellariae extract;
(4) in the mixed liquor of Flos Lonicerae extract and Radix Scutellariae extract, add water to full dose, stir evenly after high speed centrifuge with 30000rpm/min separate clear liquid, it is that 80000 ultrafilter membrane carries out ultrafiltration that clear liquid is selected molecular cut off for use, 45 ℃ of ultrafiltration temperature, operating pressure is 0.1Mpa, gets filtrate, embedding, sterilization, promptly.
Embodiment 3:
By by weight percentage: Flos Lonicerae extract 10%, Radix Scutellariae extract 90%, total amount 100% is done medicinal raw material, make according to the following steps:
(1), extracting honeysuckle, add mass concentration and be 15% alcohol reflux secondary, measured alcohol reflux 1 hour for 10 times that add for the first time Flos Lonicerae weight, measured alcohol reflux 1 hour for 8 times that add for the second time Flos Lonicerae weight, merge extracted twice liquid, being evaporated to 60 ℃, to survey relative densities be 1.17 clear paste, add ethanol and make and contain alcohol amount and reach 65%, left standstill 24 hours, get supernatant, being evaporated to 60 ℃, to survey relative densities be 1.23 cream, add water to 2.2 times of Flos Lonicerae weight, airtight, cold preservation is more than 24 hours, get supernatant, promptly get Flos Lonicerae extract;
(2) get Radix Scutellariae, decoct with water secondary, 8 times of water gagings that add Radix Scutellariae weight for the first time decocted 1.5 hours, 6 times of water gagings that add for the second time Radix Scutellariae weight decocted 1.5 hours, filtered merging filtrate, filtrate is concentrated into 60 ℃, and to survey relative densities be 1.2 cream, with molar concentration is the hydrochloric acid solution adjusting pH value to 1.9 of 2mol/L, and 60 ℃ are incubated 30 minutes, are cooled to 21 ℃, placed 12 hours, filter, remove supernatant, precipitate is washed till pH value to 4.0 with ethanol, 10 times of water gagings that add weight of precipitate, stirring, is 20% sodium hydroxide solution adjust pH to 7.0 with mass concentration, and the ethanol that adds after the dissolving with weight of precipitate equivalent stirs evenly, placed 12 hours, filter, filtrate is regulated pH value to 1.9 with molar concentration for the 2mol/L hydrochloric acid solution, and 80 ℃ are incubated 30 minutes, be cooled to 21 ℃, filter, remove supernatant, precipitate is washed till pH value to 4.0 with ethanol, drying under reduced pressure, pulverize, cross 80 mesh sieves, promptly get Radix Scutellariae extract;
(3) Radix Scutellariae extract adds 8 times of water gaging dissolvings of Radix Scutellariae extract weight, it with mass concentration 8% sodium hydroxide solution adjust pH to 8.0, filter, filtrate and Flos Lonicerae extract merge, get mixed liquor, with mass concentration is the pH value to 7.2 that 8% sodium hydroxide solution is transferred mixed liquor, boiled 1 hour, filter, add 25% simple syrup of above-mentioned mixed liquor weight, add water to 96% of full dose weight, stir evenly, with mass concentration is 8% sodium hydroxide solution adjust pH to 7.2, gets the mixed liquor of Flos Lonicerae extract and Radix Scutellariae extract;
(4) in the mixed liquor of Flos Lonicerae extract and Radix Scutellariae extract, add water to full dose, stir evenly after high speed centrifuge with 20000rpm/min separate clear liquid, it is that 60000 ultrafilter membrane carries out ultrafiltration that clear liquid is selected molecular cut off for use, 28 ℃ of ultrafiltration temperature, operating pressure is 0.2Mpa, gets filtrate, embedding, sterilization, promptly.
Concentrated Yinhuang oral liquid with preparation 50L is an example, and its step is as follows:
Embodiment 4:
(1), extracting honeysuckle 35kg, add mass concentration and be 15% alcohol reflux secondary, measured alcohol reflux 1 hour for 10 times that add for the first time Flos Lonicerae weight, measured alcohol reflux 1 hour for 8 times that add for the second time Flos Lonicerae weight, merge extracted twice liquid, being evaporated to 60 ℃, to survey relative densities be 1.16 clear paste, add ethanol and make and contain alcohol amount and reach 65%, left standstill 24 hours, get supernatant, being evaporated to 60 ℃, to survey relative densities be 1.22 cream, add water to 75kg, airtight, cold preservation is more than 24 hours, get supernatant, promptly get Flos Lonicerae extract;
(2) get Radix Scutellariae 57kg, decoct with water secondary, 8 times of water gagings that add Radix Scutellariae weight for the first time decocted 1.5 hours, 6 times of water gagings that add for the second time Radix Scutellariae weight decocted 1.5 hours, filtered merging filtrate, the concentrated 60 ℃ of survey relative densities of filtrate are 1.18 cream, with molar concentration is the hydrochloric acid solution adjusting pH value to 1.9 of 2mol/L, and 60 ℃ are incubated 30 minutes, are cooled to 22 ℃, placed 12 hours, filter, remove supernatant, precipitate is washed till pH value to 4.0 with ethanol, 10 times of water gagings that add weight of precipitate, stirring, is 20% sodium hydroxide solution adjust pH to 7.0 with mass concentration, and the ethanol that adds after the dissolving with weight of precipitate equivalent stirs evenly, placed 12 hours, filter, filtrate is regulated pH value to 1.9 with molar concentration for the 2mol/L hydrochloric acid solution, and 80 ℃ are incubated 30 minutes, be cooled to 22 ℃, filter, remove supernatant, precipitate is washed till pH value to 4.0 with ethanol, drying under reduced pressure, pulverize, cross 100 mesh sieves, promptly get Radix Scutellariae extract;
(3) get Radix Scutellariae extract 2.4kg, Flos Lonicerae extract 0.24kg, at first, in Radix Scutellariae extract, add 8 times of water gaging dissolvings of Radix Scutellariae extract weight, it with mass concentration 8% sodium hydroxide solution adjust pH to 8.0, filter, filtrate and Flos Lonicerae extract 0.24kg merge, mixed liquor, be the pH value to 7.2 of 8% sodium hydroxide solution accent mixed liquor with mass concentration, boiled 1 hour, filter, add 25% simple syrup of above-mentioned mixed liquor weight, add water to 97% of full dose weight, stir evenly, with mass concentration is 8% sodium hydroxide solution adjust pH to 7.2, gets the mixed liquor of Flos Lonicerae extract and Radix Scutellariae extract;
(4) in the mixed liquor of Flos Lonicerae extract and Radix Scutellariae extract, add water to 50L, stir evenly after high speed centrifuge with 20000rpm/min separate clear liquid, it is that 60000 ultrafilter membrane carries out ultrafiltration that clear liquid is selected molecular cut off for use, 27 ℃ of ultrafiltration temperature, operating pressure is 0.2Mpa, gets filtrate, embedding, sterilization promptly gets the 50L concentrated Yinhuang oral liquid.
Concentrated Yinhuang oral liquid of the present invention is to develop on the basis of YINHUANG KOUFUYE, and both compare has following difference:
| The top order | The plain edition YINHUANG KOUFUYE | Concentrated Yinhuang oral liquid |
| Character | This product is the supernatant liquid of rufous; Sweet, little hardship of distinguishing the flavor of. | This product is the supernatant liquid of rufous; Sweet, little hardship of distinguishing the flavor of. |
| Content | Contain chlorogenic acid 〉=1.7mg/ml, contain baicalin 〉=18.0mg/ml. | Contain chlorogenic acid 〉=3.8mg/ml, contain baicalin 〉=40.0mg/ml. |
| Specification | 10ml * 10/box | 10ml * 10/box |
| Usage and dosage | A 10～20ml, 3 times on the one; One box available 3 days at most. | A 5～10ml, 3 times on the one; One box available 6 days at most. |
By as can be seen from the above table, concentrated Yinhuang oral liquid of the present invention is easy to use, and curative effect is outstanding, can eliminate symptom better, and it is about 40% that day medication amount of money has also reduced, and plays less the effect of spending, early curing.
Concentrated Yinhuang oral liquid of the present invention is compared with existing oral liquid, and its quality and effect all increase significantly, and relevant clinical data is as follows:
One, case is selected
Every at outpatient service or inpatient, be diagnosed as affection due to external wind and heat, exuberant lung-stomach heat patient, all as the object of selecting case.Totally 77 people, test group 43 people, matched group 34 people.
Two, diagnostic criteria
Primary symptom: heating, halitosis xerostomia, have sore throat, the expectoration Huang is thick, rhinorrhea, yellowish urine constipation.
Inferior disease: micro evil wind is cold, face erythra inflammation, thin white fur of tongue or little Huang, floating and rapid pulse.
Possess 2 of above primary symptoms, 3 of inferior diseases can be diagnosed sick for this reason.
Three, exclusion standard:
1, hypertension, heart disease, hepatopathy, diabetes, nephropathy chronic diseases severe patient.
2, the Deficiency and coldness of spleen and stomach person of having loose bowels.
3, sneeze, stuffy nose with watery discharge, aversion to cold does not generate heat or generates heat not very, and is lossless, malasia, cough expectorant white matter is rare, floating and tense pulse.
4, anemia of pregnant woman, women breast-feeding their children.
5, to this product allergy sufferers, allergic constitution person.
Four, therapeutic scheme
Adopt the method for random packet, double blind control.
Test group (A): oral concentrated Yinhuang oral liquid of the present invention, obey 5ml at every turn, obey every day 3 times.
Matched group (B): oral common YINHUANG KOUFUYE, obey 10ml at every turn, obey every day 3 times.
Before taking medicine and take medicine after a week, check symptom, Chest X-rays or X-ray film, blood, urine, just routine etc. respectively.
Five, treatment criterion
Clinical every observation index calculates the score value of treatment front and back all by " new Chinese medicine clinical research guideline " rank scores standard, calculates after the treatment again and the preceding ratio of treatment, does and judges evaluation.
Recovery from illness: ratio≤0.2;
Produce effects: 0.2＜ratio≤0.5;
Effectively: 0.5＜ratio≤0.8;
Invalid: ratio＞0.8.
Six, clinical observation situation
Concentrated type (A) and plain edition (B) antivirus oral liquid clinical observation situation:
Seven, conclusion
From the above, concentrated Yinhuang oral liquid total effective rate of the present invention is than plain edition YINHUANG KOUFUYE height, and the cure rate of concentrated type of the present invention and obvious effective rate all are higher than plain edition, illustrate through behind the impurity removal and purification, the medicine inherent quality improves, the quality and the curative effect of the concentrated anti-virus oral administration liquid of the present invention's preparation all are better than the oral liquid that former preparation technology prepares, and are the innovations greatly on the preparation oral liquid method, and huge economic and social benefit is arranged.
Claims (5)
1. the preparation method of a concentrated Yinhuang oral liquid is characterized in that, by by weight percentage: Flos Lonicerae extract 5-15%, Radix Scutellariae extract 85-95%, total amount 100% is done medicinal raw material, make according to the following steps:
(1), extracting honeysuckle, add mass concentration and be 15% alcohol reflux secondary, measured alcohol reflux 1 hour for 10 times that add for the first time Flos Lonicerae weight, measured alcohol reflux 1 hour for 8 times that add for the second time Flos Lonicerae weight, merge extracted twice liquid, being evaporated to 60 ℃, to survey relative densities be 1.15～1.18 clear paste, add ethanol and make and contain alcohol amount and reach 65%, left standstill 24 hours, get supernatant, being evaporated to 60 ℃, to survey relative densities be 1.20～1.24 cream, the 2-2.5 that adds water to Flos Lonicerae weight doubly, airtight, cold preservation is more than 24 hours, get supernatant, promptly get Flos Lonicerae extract;
(2) get Radix Scutellariae, decoct with water secondary, 8 times of water gagings that add Radix Scutellariae weight for the first time decocted 1.5 hours, 6 times of water gagings that add for the second time Radix Scutellariae weight decocted 1.5 hours, filtered merging filtrate, filtrate is concentrated into 60 ℃, and to survey relative densities be 1.1～1.3 cream, with molar concentration is that the hydrochloric acid solution of 2mol/L is regulated pH value to 1.8～2.0,60 ℃ insulation 30 minutes, is cooled to 18-25 ℃, placed 12 hours, filter, remove supernatant, precipitate is washed till pH value to 4.0 with ethanol, 10 times of water gagings that add weight of precipitate, stirring, is 20% sodium hydroxide solution adjust pH to 7.0 with mass concentration, and the ethanol that adds after the dissolving with weight of precipitate equivalent stirs evenly, placed 12 hours, filter, filtrate is regulated pH value to 1.8～2.0,80 ℃ insulation 30 minutes with molar concentration for the 2mol/L hydrochloric acid solution, be cooled to 18-25 ℃, filter, remove supernatant, precipitate is washed till pH value to 4.0 with ethanol, drying under reduced pressure, pulverize, cross 80 orders or 100 mesh sieves, promptly get Radix Scutellariae extract;
(3) Radix Scutellariae extract adds 8 times of water gaging dissolvings of Radix Scutellariae extract weight, it with mass concentration 8% sodium hydroxide solution adjust pH to 8.0, filter, filtrate and Flos Lonicerae extract merge, get mixed liquor, with mass concentration is the pH value to 7.2 that 8% sodium hydroxide solution is transferred mixed liquor, boiled 1 hour, filter, the simple syrup that adds the 20%-30% of above-mentioned mixed liquor weight adds water to the 95%-98% of full dose weight, stirs evenly, with mass concentration is 8% sodium hydroxide solution adjust pH to 7.2, gets the mixed liquor of Flos Lonicerae extract and Radix Scutellariae extract;
(4) in the mixed liquor of Flos Lonicerae extract and Radix Scutellariae extract, add water to full dose, stir evenly after high speed centrifuge with 10000rpm/min-30000rpm/min separate clear liquid, it is that the ultrafilter membrane of 40000-80000 carries out ultrafiltration that clear liquid is selected molecular cut off for use, 10-45 ℃ of ultrafiltration temperature, operating pressure is 0.1-0.3Mpa, gets filtrate, embedding, sterilization, promptly.
2. the preparation method of concentrated Yinhuang oral liquid according to claim 1 is characterized in that, by by weight percentage: Flos Lonicerae extract 5%, Radix Scutellariae extract 95%, total amount 100% is done medicinal raw material, make according to the following steps:
(1), extracting honeysuckle, add mass concentration and be 15% alcohol reflux secondary, measured alcohol reflux 1 hour for 10 times that add for the first time Flos Lonicerae weight, measured alcohol reflux 1 hour for 8 times that add for the second time Flos Lonicerae weight, merge extracted twice liquid, being evaporated to 60 ℃, to survey relative densities be 1.15 clear paste, add ethanol and make and contain alcohol amount and reach 65%, left standstill 24 hours, get supernatant, being evaporated to 60 ℃, to survey relative densities be 1.20 cream, add water to 2 times of Flos Lonicerae weight, airtight, cold preservation is more than 24 hours, get supernatant, promptly get Flos Lonicerae extract;
(2) get Radix Scutellariae, decoct with water secondary, 8 times of water gagings that add Radix Scutellariae weight for the first time decocted 1.5 hours, 6 times of water gagings that add for the second time Radix Scutellariae weight decocted 1.5 hours, filtered merging filtrate, filtrate is concentrated into 60 ℃, and to survey relative densities be 1.1 cream, with molar concentration is the hydrochloric acid solution adjusting pH value to 1.8 of 2mol/L, and 60 ℃ are incubated 30 minutes, are cooled to 18 ℃, placed 12 hours, filter, remove supernatant, precipitate is washed till pH value to 4.0 with ethanol, 10 times of water gagings that add weight of precipitate, stirring, is 20% sodium hydroxide solution adjust pH to 7.0 with mass concentration, and the ethanol that adds after the dissolving with weight of precipitate equivalent stirs evenly, placed 12 hours, filter, filtrate is regulated pH value to 1.8 with molar concentration for the 2mol/L hydrochloric acid solution, and 80 ℃ are incubated 30 minutes, be cooled to 18 ℃, filter, remove supernatant, precipitate is washed till pH value to 4.0 with ethanol, drying under reduced pressure, pulverize, cross 80 mesh sieves, promptly get Radix Scutellariae extract;
(3) Radix Scutellariae extract adds 8 times of water gaging dissolvings of Radix Scutellariae extract weight, it with mass concentration 8% sodium hydroxide solution adjust pH to 8.0, filter, filtrate and Flos Lonicerae extract merge, get mixed liquor, with mass concentration is the pH value to 7.2 that 8% sodium hydroxide solution is transferred mixed liquor, boiled 1 hour, filter, add 20% simple syrup of above-mentioned mixed liquor weight, add water to 98% of full dose weight, stir evenly, with mass concentration is 8% sodium hydroxide solution adjust pH to 7.2, gets the mixed liquor of Flos Lonicerae extract and Radix Scutellariae extract;
(4) in the mixed liquor of Flos Lonicerae extract and Radix Scutellariae extract, add water to full dose, stir evenly after high speed centrifuge with 10000rpm/min separate clear liquid, it is that 40000 ultrafilter membrane carries out ultrafiltration that clear liquid is selected molecular cut off for use, 10 ℃ of ultrafiltration temperature, operating pressure is 0.3Mpa, gets filtrate, embedding, sterilization, promptly.
3. the preparation method of concentrated Yinhuang oral liquid according to claim 1 is characterized in that, by by weight percentage: Flos Lonicerae extract 15%, Radix Scutellariae extract 85%, total amount 100% is done medicinal raw material, make according to the following steps:
(1), extracting honeysuckle, add mass concentration and be 15% alcohol reflux secondary, measured alcohol reflux 1 hour for 10 times that add for the first time Flos Lonicerae weight, measured alcohol reflux 1 hour for 8 times that add for the second time Flos Lonicerae weight, merge extracted twice liquid, being evaporated to 60 ℃, to survey relative densities be 1.18 clear paste, add ethanol and make and contain alcohol amount and reach 65%, left standstill 24 hours, get supernatant, being evaporated to 60 ℃, to survey relative densities be 1.24 cream, add water to 2.5 times of Flos Lonicerae weight, airtight, cold preservation is more than 24 hours, get supernatant, promptly get Flos Lonicerae extract;
(2) get Radix Scutellariae, decoct with water secondary, 8 times of water gagings that add Radix Scutellariae weight for the first time decocted 1.5 hours, 6 times of water gagings that add for the second time Radix Scutellariae weight decocted 1.5 hours, filtered merging filtrate, filtrate is concentrated into 60 ℃, and to survey relative densities be 1.3 cream, with molar concentration is the hydrochloric acid solution adjusting pH value to 2.0 of 2mol/L, and 60 ℃ are incubated 30 minutes, are cooled to 25 ℃, placed 12 hours, filter, remove supernatant, precipitate is washed till pH value to 4.0 with ethanol, 10 times of water gagings that add weight of precipitate, stirring, is 20% sodium hydroxide solution adjust pH to 7.0 with mass concentration, and the ethanol that adds after the dissolving with weight of precipitate equivalent stirs evenly, placed 12 hours, filter, filtrate is regulated pH value to 2.0 with molar concentration for the 2mol/L hydrochloric acid solution, and 80 ℃ are incubated 30 minutes, be cooled to 25 ℃, filter, remove supernatant, precipitate is washed till pH value to 4.0 with ethanol, drying under reduced pressure, pulverize, cross 100 mesh sieves, promptly get Radix Scutellariae extract;
(3) Radix Scutellariae extract adds 8 times of water gaging dissolvings of Radix Scutellariae extract weight, it with mass concentration 8% sodium hydroxide solution adjust pH to 8.0, filter, filtrate and Flos Lonicerae extract merge, get mixed liquor, with mass concentration is the pH value to 7.2 that 8% sodium hydroxide solution is transferred mixed liquor, boiled 1 hour, filter, add 30% simple syrup of above-mentioned mixed liquor weight, add water to 95% of full dose weight, stir evenly, with mass concentration is 8% sodium hydroxide solution adjust pH to 7.2, gets the mixed liquor of Flos Lonicerae extract and Radix Scutellariae extract;
(4) in the mixed liquor of Flos Lonicerae extract and Radix Scutellariae extract, add water to full dose, stir evenly after high speed centrifuge with 30000rpm/min separate clear liquid, it is that 80000 ultrafilter membrane carries out ultrafiltration that clear liquid is selected molecular cut off for use, 45 ℃ of ultrafiltration temperature, operating pressure is 0.1Mpa, gets filtrate, embedding, sterilization, promptly.
4. the preparation method of concentrated Yinhuang oral liquid according to claim 1 is characterized in that, by by weight percentage: Flos Lonicerae extract 10%, Radix Scutellariae extract 90%, total amount 100% is done medicinal raw material, make according to the following steps:
(1), extracting honeysuckle, add mass concentration and be 15% alcohol reflux secondary, measured alcohol reflux 1 hour for 10 times that add for the first time Flos Lonicerae weight, measured alcohol reflux 1 hour for 8 times that add for the second time Flos Lonicerae weight, merge extracted twice liquid, being evaporated to 60 ℃, to survey relative densities be 1.17 clear paste, add ethanol and make and contain alcohol amount and reach 65%, left standstill 24 hours, get supernatant, being evaporated to 60 ℃, to survey relative densities be 1.23 cream, add water to 2.2 times of Flos Lonicerae weight, airtight, cold preservation is more than 24 hours, get supernatant, promptly get Flos Lonicerae extract;
(2) get Radix Scutellariae, decoct with water secondary, 8 times of water gagings that add Radix Scutellariae weight for the first time decocted 1.5 hours, 6 times of water gagings that add for the second time Radix Scutellariae weight decocted 1.5 hours, filtered merging filtrate, filtrate is concentrated into 60 ℃, and to survey relative densities be 1.2 cream, with molar concentration is the hydrochloric acid solution adjusting pH value to 1.9 of 2mol/L, and 60 ℃ are incubated 30 minutes, are cooled to 21 ℃, placed 12 hours, filter, remove supernatant, precipitate is washed till pH value to 4.0 with ethanol, 10 times of water gagings that add weight of precipitate, stirring, is 20% sodium hydroxide solution adjust pH to 7.0 with mass concentration, and the ethanol that adds after the dissolving with weight of precipitate equivalent stirs evenly, placed 12 hours, filter, filtrate is regulated pH value to 1.9 with molar concentration for the 2mol/L hydrochloric acid solution, and 80 ℃ are incubated 30 minutes, be cooled to 21 ℃, filter, remove supernatant, precipitate is washed till pH value to 4.0 with ethanol, drying under reduced pressure, pulverize, cross 80 mesh sieves, promptly get Radix Scutellariae extract;
(3) Radix Scutellariae extract adds 8 times of water gaging dissolvings of Radix Scutellariae extract weight, it with mass concentration 8% sodium hydroxide solution adjust pH to 8.0, filter, filtrate and Flos Lonicerae extract merge, get mixed liquor, with mass concentration is the pH value to 7.2 that 8% sodium hydroxide solution is transferred mixed liquor, boiled 1 hour, filter, add 25% simple syrup of above-mentioned mixed liquor weight, add water to 96% of full dose weight, stir evenly, with mass concentration is 8% sodium hydroxide solution adjust pH to 7.2, gets the mixed liquor of Flos Lonicerae extract and Radix Scutellariae extract;
(4) in the mixed liquor of Flos Lonicerae extract and Radix Scutellariae extract, add water to full dose, stir evenly after high speed centrifuge with 20000rpm/min separate clear liquid, it is that 60000 ultrafilter membrane carries out ultrafiltration that clear liquid is selected molecular cut off for use, 28 ℃ of ultrafiltration temperature, operating pressure is 0.2Mpa, gets filtrate, embedding, sterilization, promptly.
5. the preparation method of concentrated Yinhuang oral liquid according to claim 1 is characterized in that, said concentrated Yinhuang oral liquid 50L, and its step is as follows:
(1), extracting honeysuckle 35kg, add mass concentration and be 15% alcohol reflux secondary, measured alcohol reflux 1 hour for 10 times that add for the first time Flos Lonicerae weight, measured alcohol reflux 1 hour for 8 times that add for the second time Flos Lonicerae weight, merge extracted twice liquid, being evaporated to 60 ℃, to survey relative densities be 1.16 clear paste, add ethanol and make and contain alcohol amount and reach 65%, left standstill 24 hours, get supernatant, being evaporated to 60 ℃, to survey relative densities be 1.22 cream, add water to 75kg, airtight, cold preservation is more than 24 hours, get supernatant, promptly get Flos Lonicerae extract;
(2) get Radix Scutellariae 57kg, decoct with water secondary, 8 times of water gagings that add Radix Scutellariae weight for the first time decocted 1.5 hours, 6 times of water gagings that add for the second time Radix Scutellariae weight decocted 1.5 hours, filtered merging filtrate, the concentrated 60 ℃ of survey relative densities of filtrate are 1.18 cream, with molar concentration is the hydrochloric acid solution adjusting pH value to 1.9 of 2mol/L, and 60 ℃ are incubated 30 minutes, are cooled to 22 ℃, placed 12 hours, filter, remove supernatant, precipitate is washed till pH value to 4.0 with ethanol, 10 times of water gagings that add weight of precipitate, stirring, is 20% sodium hydroxide solution adjust pH to 7.0 with mass concentration, and the ethanol that adds after the dissolving with weight of precipitate equivalent stirs evenly, placed 12 hours, filter, filtrate is regulated pH value to 1.9 with molar concentration for the 2mol/L hydrochloric acid solution, and 80 ℃ are incubated 30 minutes, be cooled to 22 ℃, filter, remove supernatant, precipitate is washed till pH value to 4.0 with ethanol, drying under reduced pressure, pulverize, cross 100 mesh sieves, promptly get Radix Scutellariae extract;
(3) get Radix Scutellariae extract 2.4kg, Flos Lonicerae extract 0.24kg, at first, in Radix Scutellariae extract, add 8 times of water gaging dissolvings of Radix Scutellariae extract weight, it with mass concentration 8% sodium hydroxide solution adjust pH to 8.0, filter, filtrate and Flos Lonicerae extract 0.24kg merge, mixed liquor, be the pH value to 7.2 of 8% sodium hydroxide solution accent mixed liquor with mass concentration, boiled 1 hour, filter, add 25% simple syrup of above-mentioned mixed liquor weight, add water to 97% of full dose weight, stir evenly, with mass concentration is 8% sodium hydroxide solution adjust pH to 7.2, gets the mixed liquor of Flos Lonicerae extract and Radix Scutellariae extract;
(4) in the mixed liquor of Flos Lonicerae extract and Radix Scutellariae extract, add water to 50L, stir evenly after high speed centrifuge with 20000rpm/min separate clear liquid, it is that 60000 ultrafilter membrane carries out ultrafiltration that clear liquid is selected molecular cut off for use, 27 ℃ of ultrafiltration temperature, operating pressure is 0.2Mpa, gets filtrate, embedding, sterilization promptly gets the 50L concentrated Yinhuang oral liquid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010101884868A CN101837047B (en) | 2010-06-01 | 2010-06-01 | Method for preparing concentrated Yinhuang oral liquid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010101884868A CN101837047B (en) | 2010-06-01 | 2010-06-01 | Method for preparing concentrated Yinhuang oral liquid |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101837047A CN101837047A (en) | 2010-09-22 |
| CN101837047B true CN101837047B (en) | 2011-08-03 |
Family
ID=42740883
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010101884868A Active CN101837047B (en) | 2010-06-01 | 2010-06-01 | Method for preparing concentrated Yinhuang oral liquid |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101837047B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106237093A (en) * | 2016-08-29 | 2016-12-21 | 四川省欧邦动物药业有限公司 | A kind of silver yellow extract injection and preparation method thereof |
| CN107308209A (en) * | 2017-06-27 | 2017-11-03 | 齐全农牧集团股份有限公司 | A kind of silver yellow extract and preparation method thereof |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101032528A (en) * | 2006-03-09 | 2007-09-12 | 孔焕宇 | Discrete pharmaceutical technique for prescription of Chinese medicine film |
| CN101380385A (en) * | 2008-10-20 | 2009-03-11 | 曹长城 | Yinqin oral liquid preparation method |
-
2010
- 2010-06-01 CN CN2010101884868A patent/CN101837047B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN101837047A (en) | 2010-09-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN1899550A (en) | External plaster for treating child cold type cough and preparing method | |
| CN103893252A (en) | Composition capable of clearing heat from throat and relieving sore throat and preparation method and application thereof | |
| CN101543594B (en) | Mulberry-chrysanthemum oral liquid | |
| CN101982193A (en) | Traditional Chinese medicine composition for treating pulmonary tuberculosis, preparation method and application thereof | |
| CN101837047B (en) | Method for preparing concentrated Yinhuang oral liquid | |
| CN102579632A (en) | Preparation method of concentrated heat-clearing and cough-relieving oral solution for children | |
| CN101411493B (en) | Cold tea for preventing and treating fatigue type sub-health and preparation method thereof | |
| CN105288134B (en) | Preparation method of pear paste for moistening lung | |
| WO2011095095A1 (en) | Medicinal composition comprising alcohol-soluble and water-insoluble licorice extract, pharmaceutical preparation, pharmaceutical application, therapeutic method, and preparative method thereof | |
| CN106974981A (en) | A kind of external used medicine of analgesic | |
| CN108030887A (en) | A kind of Chinese materia medica syrup for being used to treat cough variant asthma in childhood: prednison comparing | |
| CN101129739A (en) | Oral liquid of carapax trionycis and asparagus | |
| CN102631486B (en) | Health care composition | |
| CN104940428B (en) | Hypoglycemic composition containing wolfberry fruits and application thereof | |
| CN108837045A (en) | A kind of preparation method for paediatric acute tonsillitis, the Chinese medicine composition of acpuei pharyngitis | |
| CN105250919B (en) | It is a kind of for preventing and/or the Chinese medicine composition for treating liver disease and preparation method thereof | |
| CN102973657A (en) | Scutellaria baicalensis, manchurian lilac bark and dahurian rhododendron leaf cough-arresting mixture and preparation method thereof | |
| CN101934026B (en) | Chinese medicinal composition for treating cough and asthma and preparation method thereof | |
| CN102813806A (en) | Traditional Chinese medicine for treating lung heat and body fluid impairment type diabetes | |
| CN102836315A (en) | Chinese medicinal composition for preventing and conditioning prognosis tumors and preparation method thereof | |
| CN101991721B (en) | Shiqi expathy traditional Chinese medicine sugar-free granule | |
| CN109091633B (en) | Composition for treating recurrent respiratory tract infection of children and preparation method and application thereof | |
| CN101167762B (en) | Method for preparing health-care capsule with mutation-resisting and immunoregulating function | |
| CN113288969A (en) | Medicine for treating bronchitis and preparation method thereof | |
| CN106108009A (en) | Herba Cirsii extracting solution, health product containing Herba Cirsii active component and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |