CN101828516A - Method for realizing mechanical production of crop hybridization seeds by using fluorescent protein gene markers - Google Patents
Method for realizing mechanical production of crop hybridization seeds by using fluorescent protein gene markers Download PDFInfo
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- CN101828516A CN101828516A CN200910103336A CN200910103336A CN101828516A CN 101828516 A CN101828516 A CN 101828516A CN 200910103336 A CN200910103336 A CN 200910103336A CN 200910103336 A CN200910103336 A CN 200910103336A CN 101828516 A CN101828516 A CN 101828516A
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Abstract
The invention relates to a method for realizing mechanical production of crop hybridization seeds by using fluorescent protein gene markers. Fluorescent protein gene used as a marking trait is introduced into a hybridized combination parent through a transgenosis and hybridization mode, plants the seeds of which have enough identification expression of the fluorescent protein are selected to reproduce so as to breed a parent with the fluorescent protein gene. When the first generation seeds of the hybridization seeds are produced, the parent seeds are mixed according to a needed proportion and sowed. After the hybridization seeds are ripe, a mechanical mixing method is adopted for harvest, and the first-filial generation seeds are separated and collected by using a color-selection machine with fluorescent identification. By adopting the production method of the hybridization seeds, the whole-course mechanization of the hybridization seed production can be truly realized, the production cost of the hybridization seeds can be greatly reduced, the production efficiency of the hybridization seeds is greatly improved, the purity of the hybridization seeds can be ensured, and the hybridization seeds with great amount, high purity and low cost are provided for crop producers.
Description
Technical field
The present invention does the method that the seed identification marking realizes the production of crop hybridization seeds tool for a kind of fluorescence protein gene that adopts.
Background technology
World today's crop hybrid superiority utilization has been the important channel of improving crop yield, the production of hybrid seed is the heterosis utilization important step, and reducing the production cost of hybrid seed and improving hybrid seed purity is to realize the crucial key element of heterosis utilization.Extensively promoted hybrid seed at China's paddy rice corn, but, plantation can not carry out mechanization production because producing, the production cost height, it is higher that the peasant uses hybrid seed to pay: though crops hybrid seed productions such as wheat, soybean success causes scientific and technological achievement can't carry out large scale application because of production cost is too high.Realize the mechanization production of hybrid seed, reduce the production cost of hybrid seed, become the active demand that seed production is already enhanced competitiveness.
Summary of the invention
The present invention does the method that the seed identification marking realizes the production of crop hybridization seeds tool for a kind of fluorescence protein gene that adopts.Concrete grammar is: fluorescence protein gene serves as a mark among the parent of proterties by the combination of transgenosis and Crossing system introductive crossing, selects that fluorescin has the plant of enough recognition expression amounts to breed in the seed, cultivates the parent with fluorescence protein gene.When producing hybrid seed generation kind, with this Parent desired proportions mixed seeding (florescence asynchronous must differ from the phase mixed seeding).Carry out the mechanical mixture harvesting after the hybrid seed maturation, with the color selector tool of fluorescence identification light source first-filial generation seed separated and collected being processed.Adopt this hybrid seed production method, can really realize the entire mechanization that hybrid seed is produced, can significantly reduce the hybrid seed production cost, significantly improve hybrid seed production efficiency again, can also guarantee the purity of hybrid seed, the high low price hybrid seed of the big purity of crop production person's amount of providing is provided.
Embodiment
Adopt fluorescence protein gene to do the method that the seed identification marking realizes the production of crop hybridization seeds tool, to change hybrid seed production needs in a large number manually, and productivity ratio is low, the conventional approach that cost is high, can significantly improve hybrid seed production efficiency, reduce the cost of producing hybrid seed.
Embodiment one, can in seed, there be the fluorescence protein gene of enough expressions to change in the sterile line with a kind of, adopt not the male parent with fluorescence protein gene, because maternal male-sterility, the solid seed of maternal institute is the hybrid seed with fluorescence protein gene entirely, employing is processed first-filial generation seed separated and collected with the color selector tool of fluorescence identification light source, just can obtain the very high hybrid seed of purity.
Embodiment two, can in seed, there be the fluorescence protein gene of enough expressions to change in the sterile line with a kind of, employing is with the male parent of another kind of fluorescence protein gene, the solid seed of maternal institute is the hybrid seed of band female parent and two kinds of fluorescence protein genes of male parent entirely, adopting band can identify the color selector tool of variety classes fluorescence with the seed separating treatment of different fluorescence, is exactly required hybrid seed with the seed of maternal fluorescence.
Embodiment three, can in seed, there be the fluorescence protein gene of certain expression to change in the sterile line with a kind of, employing is with fluorescence protein gene of the same race but expression and maternal discrepant male parent, maternal solid hybrid seed has different luciferase expression amounts from the male parent seed, adopt the color selector tool that to identify fluorescence variable expression of the same race with first-filial generation seed separating treatment, just can obtain the very high hybrid seed of purity.
Embodiment four, selected a kind of not with the sterile line of fluorescence protein gene, adopt band fluorescence protein gene male parent, maternal solid hybrid seed has different expressions from the male parent seed, adopt and be with the color selector tool that to identify different luciferase expression amounts with first-filial generation seed separating treatment, just can obtain the very high hybrid seed of purity.
Claims (2)
1. adopt fluorescence protein gene to do the method that the seed identification marking realizes the production of crop hybridization seeds tool.Its specific features is: fluorescence protein gene is served as a mark among the parent of proterties by the combination of transgenosis and Crossing system introductive crossing, be chosen in that fluorescin has the plant of enough recognition expression amounts to breed in the seed, cultivate the parent with fluorescence protein gene; When producing hybrid seed generation kind, with this Parent desired proportions mixed seeding, carry out the mechanical mixture harvesting after the hybrid seed maturation, use the color selector tool of identifying with fluorescence with first-filial generation seed separated and collected.
2. according to claim 1, it is characterized in that: affiliated technology is distinguished hybrid seed and parental seed with fluorescent characteristic and expression, and color selector is equipped with the corresponding fluorescence light source of seed being discerned separation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910103336A CN101828516A (en) | 2009-03-09 | 2009-03-09 | Method for realizing mechanical production of crop hybridization seeds by using fluorescent protein gene markers |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910103336A CN101828516A (en) | 2009-03-09 | 2009-03-09 | Method for realizing mechanical production of crop hybridization seeds by using fluorescent protein gene markers |
Publications (1)
| Publication Number | Publication Date |
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| CN101828516A true CN101828516A (en) | 2010-09-15 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
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| CN200910103336A Pending CN101828516A (en) | 2009-03-09 | 2009-03-09 | Method for realizing mechanical production of crop hybridization seeds by using fluorescent protein gene markers |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2014154141A1 (en) * | 2013-03-29 | 2014-10-02 | 湖南杂交水稻研究中心 | Mechanized seed production method using female-sterile hybrid rice plants |
-
2009
- 2009-03-09 CN CN200910103336A patent/CN101828516A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2014154141A1 (en) * | 2013-03-29 | 2014-10-02 | 湖南杂交水稻研究中心 | Mechanized seed production method using female-sterile hybrid rice plants |
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Application publication date: 20100915 |