CN101812489A - Culture medium for producing griseofulvin by fermentation - Google Patents
Culture medium for producing griseofulvin by fermentation Download PDFInfo
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- CN101812489A CN101812489A CN 201010175051 CN201010175051A CN101812489A CN 101812489 A CN101812489 A CN 101812489A CN 201010175051 CN201010175051 CN 201010175051 CN 201010175051 A CN201010175051 A CN 201010175051A CN 101812489 A CN101812489 A CN 101812489A
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- maydis powder
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Abstract
The invention discloses a culture medium for producing fermentation by fermentation, which comprises a seed culture medium and a fermentation medium. In the process of fermentation culture, when total sugar reduces to 2.0-3.0%, a feed supplement culture medium is added; and corn is used as a main carbon source to prepare the fermentation culture medium. Preferentially, the seed culture medium and the feed supplement culture medium are also prepared by using the corn as the main carbon source. Since the price of corn flour is much lower than that of rice flour, the production cost is greatly reduced; in addition, the corn flour has sufficient supply and stable quality, thereby ensuring the stability of fermentation production.
Description
Technical field
The invention belongs to the microbial fermentation engineering technical field, relate to a kind of substratum of producing griseofulvin by fermentation specifically.
Background technology
Grisovin (griseoflvin) belongs to the microbiotic of aromatic derivant class, is a kind of antifungal antibiotic, mainly treats the infection that Pi Fu Xian Pseudomonas causes, is used for the plant epiphyte resisting disease on agricultural.The generation bacterium that the fermentative production of domestic grisovin is used all be by China from the wild Penicillumpatulum4541 bacterial strain of row filter through too much obtaining for mutagenesis and directive breeding, the bacterial classification that the present invention uses also is the offspring of this bacterial strain.Since 1880s grisovin industrial fermentation is produced, the main carbon source of long-grained nonglutinous rice powder (or claiming rice meal) conduct of always using the southern long-grained nonglutinous rice that abounds with to be processed into.Rice is produced grisovin as main carbon source, and the source of goods is inadequate, raw materials quality there is not guarantee, and the production cost height.
Summary of the invention
The objective of the invention is to overcome the shortcoming of prior art, a kind of sufficient raw is provided, the substratum of the producing griseofulvin by fermentation of low production cost.
The object of the present invention is achieved like this: a kind of substratum of producing griseofulvin by fermentation comprises seed culture medium, fermention medium and supplemented medium; It is characterized in that: described fermention medium is formed by the preparation of raw material of following weight ratio, and Semen Maydis powder 13～17%, lime carbonate 0.5～0.7%, potassium primary phosphate 0.6～1%, sodium-chlor 0.3～0.5%, Repone K 0.5～0.7%, sal epsom 0.06～0.14%, ferrous sulfate 0.06～0.14%, ammonium sulfate 0.06～0.14%, SODIUMNITRATE 0.06～0.14%, surplus are water.
As a further improvement on the present invention, described seed culture medium is formed by the preparation of raw material of following weight ratio, and Semen Maydis powder 8～12%, lime carbonate 0.3～0.5%, potassium primary phosphate 0.3～0.5%, sodium-chlor 0.15～0.25%, Repone K 0.15～0.25%, sal epsom 0.06～0.14%, ferrous sulfate 0.06～0.14%, ammonium sulfate 0.06～0.14%, SODIUMNITRATE 0.06～0.14%, surplus are water.
Further improve as of the present invention, described supplemented medium is formed by the preparation of raw material of following weight ratio, and Semen Maydis powder 15～40%, amylase 0.02～0.1%, foam killer 0.05～0.3%, surplus are water.
Described Semen Maydis powder is to select yellow gingival cyst of mucous gland in the newborn corn for use, and perhaps flour corn forms through the embryo of peeling, take off, pulverizing; Fineness 80 orders of Semen Maydis powder sieve more than 60%, and sugared content is more than 80%, and protein content is less than 9%, and moisture is less than 9%.
Described fermention medium, seed culture medium and supplemented medium, preparation method separately takes by weighing each raw material according to the above ratio, drops in the fermentor tank, stirs, and seals sterilization and makes.
The invention has the advantages that: the substratum of producing griseofulvin by fermentation of the present invention, use the abundant corn of produce to be processed into Semen Maydis powder as raw material, substitute long-term raw material with the rice meal producing griseofulvin by fermentation, produce grisovin by fermentation.Its advantage be following some: the Semen Maydis powder price is lower than rice meal, reduces the production cost of company greatly; The ample supply and prompt delivery of Semen Maydis powder, steady quality guarantees the stable of fermentative production; Purchasing department can purchase corn nearby, reduces purchase cost; Logistics department does not need large quantities of transportation by railroad rice, has alleviated transport pressure.
Embodiment
Embodiment 1: a kind of substratum of producing griseofulvin by fermentation, comprise seed culture medium, fermention medium and supplemented medium, described seed culture medium is formed by the preparation of raw material of following weight ratio, and Semen Maydis powder 10%, lime carbonate 0.4%, potassium primary phosphate 0.4%, sodium-chlor 0.2%, Repone K 0.2%, sal epsom 0.1%, ferrous sulfate 0.1%, ammonium sulfate 0.1%, SODIUMNITRATE 0.1%, surplus are water; Described fermention medium is formed by the preparation of raw material of following weight ratio, and Semen Maydis powder 15%, lime carbonate 0.6%, potassium primary phosphate 0.8%, sodium-chlor 0.4%, Repone K 0.6%, sal epsom 0.1%, ferrous sulfate 0.1%, ammonium sulfate 0.1%, SODIUMNITRATE 0.1%, surplus are water; Described supplemented medium is formed by the preparation of raw material of following weight ratio, and Semen Maydis powder 28%, amylase 0.05%, foam killer 0.15%, surplus are water.Wherein, described Semen Maydis powder is to select for use yellow gingival cyst of mucous gland in the newborn corn to form through the embryo of peeling, take off, pulverizing; Fineness 80 orders of Semen Maydis powder sieve more than 60%, and sugared content is more than 80%, and protein content is less than 9%, and moisture is less than 9%.
Embodiment 2: a kind of substratum of producing griseofulvin by fermentation, comprise seed culture medium, fermention medium and supplemented medium, described seed culture medium is formed by the preparation of raw material of following weight ratio, and Semen Maydis powder 9%, lime carbonate 0.35%, potassium primary phosphate 0.35%, sodium-chlor 0.16%, Repone K 0.17%, sal epsom 0.08%, ferrous sulfate 0.07%, ammonium sulfate 0.09%, SODIUMNITRATE 0.08%, surplus are water.Described fermention medium is formed by the preparation of raw material of following weight ratio, and Semen Maydis powder 13%, lime carbonate 0.55%, potassium primary phosphate 0.7%, sodium-chlor 0.35%, Repone K 0.56%, sal epsom 0.08%, ferrous sulfate 0.09%, ammonium sulfate 0.07%, SODIUMNITRATE 0.08%, surplus are water; Described supplemented medium is formed by the preparation of raw material of following weight ratio, and Semen Maydis powder 25%, amylase 0.03%, foam killer 0.1%, surplus are water.Wherein, described Semen Maydis powder is to select for use flour corn to form through the embryo of peeling, take off, pulverizing; Fineness 80 orders of Semen Maydis powder sieve more than 60%, and sugared content is more than 80%, and protein content is less than 9%, and moisture is less than 9%.
Embodiment 3: a kind of substratum of producing griseofulvin by fermentation, comprise seed culture medium, fermention medium and supplemented medium, described seed culture medium is formed by the preparation of raw material of following weight ratio, and Semen Maydis powder 11%, lime carbonate 0.42%, potassium primary phosphate 0.45%, sodium-chlor 0.22%, Repone K 0.21%, sal epsom 0.12%, ferrous sulfate 0.13%, ammonium sulfate 0.11%, SODIUMNITRATE 0.12%, surplus are water; Described fermention medium is formed by the preparation of raw material of following weight ratio, and Semen Maydis powder 17%, lime carbonate 0.65%, potassium primary phosphate 0.9%, sodium-chlor 0.45%, Repone K 0.65%, sal epsom 0.11%, ferrous sulfate 0.12%, ammonium sulfate 0.10%, SODIUMNITRATE 0.11%, surplus are water; Described supplemented medium is formed by the preparation of raw material of following weight ratio, and Semen Maydis powder 30%, amylase 0.08%, foam killer 0.2%, surplus are water.Wherein, described Semen Maydis powder is to select for use flour corn to form through the embryo of peeling, take off, pulverizing; Fineness 80 orders of Semen Maydis powder sieve more than 60%, and sugared content is more than 80%, and protein content is less than 9%, and moisture is less than 9%.
Claims (5)
1. the substratum of a producing griseofulvin by fermentation comprises seed culture medium, fermention medium and supplemented medium; It is characterized in that: described fermention medium is formed by the preparation of raw material of following weight ratio, and Semen Maydis powder 13～17%, lime carbonate 0.5～0.7%, potassium primary phosphate 0.6～1%, sodium-chlor 0.3～0.5%, Repone K 0.5～0.7%, sal epsom 0.06～0.14%, ferrous sulfate 0.06～0.14%, ammonium sulfate 0.06～0.14%, SODIUMNITRATE 0.06～0.14%, surplus are water.
2. according to the substratum of the described producing griseofulvin by fermentation of claim 1, it is characterized in that: described seed culture medium is formed by the preparation of raw material of following weight ratio, and Semen Maydis powder 8～12%, lime carbonate 0.3～0.5%, potassium primary phosphate 0.3～0.5%, sodium-chlor 0.15～0.25%, Repone K 0.15～0.25%, sal epsom 0.06～0.14%, ferrous sulfate 0.06～0.14%, ammonium sulfate 0.06～0.14%, SODIUMNITRATE 0.06～0.14%, surplus are water.
3. according to the substratum of claim 1 or 2 described producing griseofulvin by fermentation, it is characterized in that: described supplemented medium is formed by the preparation of raw material of following weight ratio, and Semen Maydis powder 15～40%, amylase 0.02～0.1%, foam killer 0.05～0.3%, surplus are water.
4. according to the substratum of claim 1 or 2 described producing griseofulvin by fermentation, it is characterized in that: described Semen Maydis powder is to select yellow gingival cyst of mucous gland in the newborn corn for use, and perhaps flour corn forms through the embryo of peeling, take off, pulverizing; Fineness 80 orders of Semen Maydis powder sieve more than 60%, and sugared content is more than 80%, and protein content is less than 9%, and moisture is less than 9%.
5. according to the substratum of the described producing griseofulvin by fermentation of claim 3, it is characterized in that: described Semen Maydis powder is to select yellow gingival cyst of mucous gland in the newborn corn for use, and perhaps flour corn forms through the embryo of peeling, take off, pulverizing; Fineness 80 orders of Semen Maydis powder sieve more than 60%, and sugared content is more than 80%, and protein content is less than 9%, and moisture is less than 9%.
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| CN 201010175051 CN101812489A (en) | 2010-04-29 | 2010-04-29 | Culture medium for producing griseofulvin by fermentation |
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| CN 201010175051 CN101812489A (en) | 2010-04-29 | 2010-04-29 | Culture medium for producing griseofulvin by fermentation |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103275047A (en) * | 2013-05-23 | 2013-09-04 | 内蒙古格林特制药有限责任公司 | Preparation method of griseofulvin |
| CN106480121A (en) * | 2016-09-27 | 2017-03-08 | 宁夏泰瑞制药股份有限公司 | A kind of culture medium of utilization penicillium patulum producing griseofulvin by fermentation and cultural method |
| CN108641969A (en) * | 2018-06-21 | 2018-10-12 | 赤峰制药股份有限公司 | A kind of griseofulvin production bacterium culture medium |
| CN110317841A (en) * | 2019-07-10 | 2019-10-11 | 内蒙古格林特制药有限责任公司 | A kind of griseofulvin fermentation production medium |
| CN110669678A (en) * | 2019-10-22 | 2020-01-10 | 赤峰制药股份有限公司 | Griseofulvin strain solid culture medium |
| CN112094874A (en) * | 2020-09-16 | 2020-12-18 | 内蒙古格林特制药有限责任公司 | Culture medium for producing griseofulvin through fermentation |
| CN115644062A (en) * | 2022-11-03 | 2023-01-31 | 天津科润农业科技股份有限公司 | Culture medium and method for improving embryo induction rate of Chinese cabbage with embryos difficult to produce |
-
2010
- 2010-04-29 CN CN 201010175051 patent/CN101812489A/en active Pending
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| 《亚热带植物通讯》 19941231 沈玉芬 等 灰黄霉素产生菌耐前体变株F-1012的选育及其发酵特性 19-25 1-5 第23卷, 第2期 2 * |
| 《药物生物技术》 19951231 林琳 等 灰黄霉素产生菌耐前体变株F-208的选育及其特性的研究 28-32 1-4 第2卷, 第4期 2 * |
| 《西南农业学报》 20051231 杨红玉 等 灰霉菌培养及其对拟南芥的侵染 431-434 1-5 第18卷, 第4期 2 * |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103275047A (en) * | 2013-05-23 | 2013-09-04 | 内蒙古格林特制药有限责任公司 | Preparation method of griseofulvin |
| CN103275047B (en) * | 2013-05-23 | 2014-09-03 | 内蒙古格林特制药有限责任公司 | Preparation method of griseofulvin |
| CN106480121A (en) * | 2016-09-27 | 2017-03-08 | 宁夏泰瑞制药股份有限公司 | A kind of culture medium of utilization penicillium patulum producing griseofulvin by fermentation and cultural method |
| CN108641969A (en) * | 2018-06-21 | 2018-10-12 | 赤峰制药股份有限公司 | A kind of griseofulvin production bacterium culture medium |
| CN110317841A (en) * | 2019-07-10 | 2019-10-11 | 内蒙古格林特制药有限责任公司 | A kind of griseofulvin fermentation production medium |
| CN110317841B (en) * | 2019-07-10 | 2022-11-22 | 内蒙古格林特制药有限责任公司 | Fermentation production culture medium for griseofulvin |
| CN110669678A (en) * | 2019-10-22 | 2020-01-10 | 赤峰制药股份有限公司 | Griseofulvin strain solid culture medium |
| CN112094874A (en) * | 2020-09-16 | 2020-12-18 | 内蒙古格林特制药有限责任公司 | Culture medium for producing griseofulvin through fermentation |
| CN115644062A (en) * | 2022-11-03 | 2023-01-31 | 天津科润农业科技股份有限公司 | Culture medium and method for improving embryo induction rate of Chinese cabbage with embryos difficult to produce |
| CN115644062B (en) * | 2022-11-03 | 2023-11-17 | 天津科润农业科技股份有限公司 | Culture medium and method for improving induction rate of embryo of difficult-to-embryo Chinese cabbage |
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Application publication date: 20100825 |