CN110317841B - Fermentation production culture medium for griseofulvin - Google Patents
Fermentation production culture medium for griseofulvin Download PDFInfo
- Publication number
- CN110317841B CN110317841B CN201910648868.5A CN201910648868A CN110317841B CN 110317841 B CN110317841 B CN 110317841B CN 201910648868 A CN201910648868 A CN 201910648868A CN 110317841 B CN110317841 B CN 110317841B
- Authority
- CN
- China
- Prior art keywords
- griseofulvin
- fermentation
- culture medium
- flour
- reduced
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- DDUHZTYCFQRHIY-RBHXEPJQSA-N griseofulvin Chemical compound COC1=CC(=O)C[C@@H](C)[C@@]11C(=O)C(C(OC)=CC(OC)=C2Cl)=C2O1 DDUHZTYCFQRHIY-RBHXEPJQSA-N 0.000 title claims abstract description 38
- DDUHZTYCFQRHIY-UHFFFAOYSA-N Negwer: 6874 Natural products COC1=CC(=O)CC(C)C11C(=O)C(C(OC)=CC(OC)=C2Cl)=C2O1 DDUHZTYCFQRHIY-UHFFFAOYSA-N 0.000 title claims abstract description 34
- 238000000855 fermentation Methods 0.000 title claims abstract description 34
- 230000004151 fermentation Effects 0.000 title claims abstract description 34
- IIUZTXTZRGLYTI-UHFFFAOYSA-N Dihydrogriseofulvin Natural products COC1CC(=O)CC(C)C11C(=O)C(C(OC)=CC(OC)=C2Cl)=C2O1 IIUZTXTZRGLYTI-UHFFFAOYSA-N 0.000 title claims abstract description 33
- UXWOXTQWVMFRSE-UHFFFAOYSA-N Griseoviridin Natural products O=C1OC(C)CC=C(C(NCC=CC=CC(O)CC(O)C2)=O)SCC1NC(=O)C1=COC2=N1 UXWOXTQWVMFRSE-UHFFFAOYSA-N 0.000 title claims abstract description 33
- 229960002867 griseofulvin Drugs 0.000 title claims abstract description 33
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 13
- 239000001963 growth medium Substances 0.000 title claims abstract description 11
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims abstract description 14
- 235000013312 flour Nutrition 0.000 claims abstract description 13
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims abstract description 12
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims abstract description 12
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract description 12
- VWDWKYIASSYTQR-UHFFFAOYSA-N sodium nitrate Chemical compound [Na+].[O-][N+]([O-])=O VWDWKYIASSYTQR-UHFFFAOYSA-N 0.000 claims abstract description 12
- 239000002994 raw material Substances 0.000 claims abstract description 8
- 229910000019 calcium carbonate Inorganic materials 0.000 claims abstract description 7
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims abstract description 7
- 235000019796 monopotassium phosphate Nutrition 0.000 claims abstract description 7
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims abstract description 7
- 244000017020 Ipomoea batatas Species 0.000 claims abstract description 6
- 235000002678 Ipomoea batatas Nutrition 0.000 claims abstract description 6
- 240000008042 Zea mays Species 0.000 claims abstract description 6
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims abstract description 6
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims abstract description 6
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims abstract description 6
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims abstract description 6
- 235000011130 ammonium sulphate Nutrition 0.000 claims abstract description 6
- 235000005822 corn Nutrition 0.000 claims abstract description 6
- 239000011790 ferrous sulphate Substances 0.000 claims abstract description 6
- 235000003891 ferrous sulphate Nutrition 0.000 claims abstract description 6
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims abstract description 6
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims abstract description 6
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims abstract description 6
- 235000019341 magnesium sulphate Nutrition 0.000 claims abstract description 6
- 239000001103 potassium chloride Substances 0.000 claims abstract description 6
- 235000011164 potassium chloride Nutrition 0.000 claims abstract description 6
- 229920001592 potato starch Polymers 0.000 claims abstract description 6
- 239000011780 sodium chloride Substances 0.000 claims abstract description 6
- 235000010344 sodium nitrate Nutrition 0.000 claims abstract description 6
- 239000004317 sodium nitrate Substances 0.000 claims abstract description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 6
- RWSXRVCMGQZWBV-WDSKDSINSA-N glutathione Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-WDSKDSINSA-N 0.000 claims description 8
- 108010024636 Glutathione Proteins 0.000 claims description 4
- 235000007164 Oryza sativa Nutrition 0.000 claims description 4
- 229960003180 glutathione Drugs 0.000 claims description 4
- 235000009566 rice Nutrition 0.000 claims description 4
- 240000007594 Oryza sativa Species 0.000 claims 1
- 240000002582 Oryza sativa Indica Group Species 0.000 abstract description 3
- 238000005265 energy consumption Methods 0.000 abstract description 3
- MBBZMMPHUWSWHV-BDVNFPICSA-N N-methylglucamine Chemical compound CNC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO MBBZMMPHUWSWHV-BDVNFPICSA-N 0.000 abstract description 2
- 229960003194 meglumine Drugs 0.000 abstract description 2
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 22
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 10
- 230000000052 comparative effect Effects 0.000 description 5
- 238000001035 drying Methods 0.000 description 5
- 238000005406 washing Methods 0.000 description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 description 4
- 230000008901 benefit Effects 0.000 description 4
- 239000012043 crude product Substances 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 238000001953 recrystallisation Methods 0.000 description 4
- 241000209094 Oryza Species 0.000 description 3
- 238000012258 culturing Methods 0.000 description 3
- 238000001914 filtration Methods 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 239000002609 medium Substances 0.000 description 3
- 208000002474 Tinea Diseases 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 238000009472 formulation Methods 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 230000000415 inactivating effect Effects 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 206010005913 Body tinea Diseases 0.000 description 1
- 241001480035 Epidermophyton Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 241001480037 Microsporum Species 0.000 description 1
- 208000010195 Onychomycosis Diseases 0.000 description 1
- 241000130764 Tinea Species 0.000 description 1
- 206010043866 Tinea capitis Diseases 0.000 description 1
- 201000010618 Tinea cruris Diseases 0.000 description 1
- 241000223238 Trichophyton Species 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- BRPQOXSCLDDYGP-UHFFFAOYSA-N calcium oxide Chemical compound [O-2].[Ca+2] BRPQOXSCLDDYGP-UHFFFAOYSA-N 0.000 description 1
- 239000000292 calcium oxide Substances 0.000 description 1
- ODINCKMPIJJUCX-UHFFFAOYSA-N calcium oxide Inorganic materials [Ca]=O ODINCKMPIJJUCX-UHFFFAOYSA-N 0.000 description 1
- 239000012531 culture fluid Substances 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000012065 filter cake Substances 0.000 description 1
- 239000006260 foam Substances 0.000 description 1
- 238000005469 granulation Methods 0.000 description 1
- 230000003179 granulation Effects 0.000 description 1
- 229960000480 griseofulvin microsize Drugs 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000011218 seed culture Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
- 201000009642 tinea barbae Diseases 0.000 description 1
- 201000003875 tinea corporis Diseases 0.000 description 1
- 201000004647 tinea pedis Diseases 0.000 description 1
- 201000005882 tinea unguium Diseases 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
- C12P17/02—Oxygen as only ring hetero atoms
- C12P17/04—Oxygen as only ring hetero atoms containing a five-membered hetero ring, e.g. griseofulvin, vitamin C
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Mycology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Botany (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Biomedical Technology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention discloses a culture medium for fermentation production of griseofulvin, which is prepared from the following raw materials in percentage by weight: 6% of indica rice flour, 5% of corn flour, 3% of sweet potato flour, 0.6% of potassium dihydrogen phosphate, 0.4% of calcium carbonate, 0.1% of sodium nitrate, 0.1% of ammonium sulfate, 0.1% of ferrous sulfate, 0.8% of sodium chloride, 0.7% of potassium chloride, 0.1% of magnesium sulfate, 0.05% of a GPE (meglumine) type, and the balance of water. The viscosity of the fermentation liquor is reduced, the fermentation unit is stabilized at 28000-30000 mu g/ml, the fermentation period is shortened by about 45 hours, the fermentation energy consumption is reduced, the cost is reduced, and the yield is high.
Description
The invention relates to a preparation method of bulk drugs, in particular to a culture medium for fermentation production of griseofulvin.
Griseofulvin is an antibiotic raw material drug with the English name Griseofulvin, the Chinese name Griseofulvin, the microcrystalline Griseofulvin, the English name Fulcin, fulcine, fulvicin, fungivin, grifukulvin, grisivin, griseofulvin, [ CAS ] 126-07-8, and the product is white or white-like fine powder; the product has good antibacterial effect on superficial fungi such as trichophyton, microsporum, epidermophyton, etc.
Griseofulvin is suitable for treating various tinea diseases including tinea capitis, tinea barbae, tinea corporis, tinea cruris, tinea pedis and tinea unguium.
In the existing method for preparing griseofulvin, a, fermentation culture of griseofulvin is carried out for a certain period of heating inactivation, solid-liquid separation of a plate frame, granulation and drying of filter cake mycelia. b. Extracting with acetone to obtain griseofulvin acetone extractive solution; c. decoloring the griseofulvin acetone extract by using activated carbon, and concentrating and crystallizing the decolored solution to obtain a griseofulvin crude product; d. dissolving the griseofulvin crude product by using acetone, decoloring the griseofulvin acetone dissolved solution by using activated carbon, and concentrating and crystallizing the decolored solution to obtain a griseofulvin recrystallization; e. washing griseofulvin recrystallization with butanol, washing with ethanol, drying, and micronizing to obtain griseofulvin raw material.
The invention aims to provide a griseofulvin fermentation production culture medium, which has the advantages of low energy consumption, cost reduction, short fermentation period, fermentation unit improvement and production efficiency improvement in the production process.
The technical scheme adopted by the invention is as follows:
a culture medium for fermentation production of griseofulvin is prepared from the following raw materials in percentage by weight: 6% of long-shaped rice flour, 5% of corn flour, 3% of sweet potato flour, 0.6% of potassium dihydrogen phosphate, 0.4% of calcium carbonate, 0.1% of sodium nitrate, 0.1% of ammonium sulfate, 0.1% of ferrous sulfate, 0.8% of sodium chloride, 0.7% of potassium chloride, 0.1% of magnesium sulfate, 0.05% of kyoto GPE (glutathione) type, and the balance of water.
The invention has the advantages that: 1. the carbon source components and the mixture ratio in the fermentation medium are adjusted, and the production process monitoring data show that the mixture ratio of the carbon source is 6% of long-shaped rice flour, 5% of corn flour and 3% of sweet potato flour, so that the viscosity of the fermentation liquid can be reduced, the fermentation unit is stabilized at 28000-30000 mu g/ml, the fermentation period is shortened by about 45 hours, the fermentation energy consumption is reduced, and the cost is reduced;
2. compared with a comparison formula, the potassium dihydrogen phosphate is adjusted to be 0.6 percent, the calcium carbonate is adjusted to be 0.4 percent, the pH value of the fermentation liquor is reduced from 6.8 to 5.8, and the growth speed of the bacterial strain in the fermentation liquor is obviously increased compared with that of the bacterial strain in the comparison test formula by sampling and observing under a microscope;
3. the addition of a proper amount of GPE (glutathione E) type foam can improve the gas-liquid mixing effect in the fermentation tank, increase the oxygen rate in the fermentation tank, and facilitate the growth of strains and increase the yield;
4. the test formula is applied to industrial mass production, and the yield is about 16 percent higher than that of a reference test formula.
The present invention will be further described below.
A culture medium for fermentation production of griseofulvin is prepared from the following raw materials in percentage by weight: 6% of long-shaped rice flour, 5% of corn flour, 3% of sweet potato flour, 0.6% of potassium dihydrogen phosphate, 0.4% of calcium carbonate, 0.1% of sodium nitrate, 0.1% of ammonium sulfate, 0.1% of ferrous sulfate, 0.8% of sodium chloride, 0.7% of potassium chloride, 0.1% of magnesium sulfate, 0.05% of kyoto GPE (glutathione) type, and the balance of water.
The method for producing griseofulvin by using the culture medium comprises the following steps:
a. the culture medium is configured and then is put into a fermentation tank at 121-126 ℃, the sterilization is maintained for 30 minutes, when the temperature of the tank is reduced to 30 ℃, the inoculation and the fermentation are carried out, and the culture conditions are as follows: initial medium pH =5.8, culture temperature: 29 ℃. + -. 1 ℃, air flow: 2500-3600 m 3 H, pot pressure: 0.01-0.05 MPa, supplementing material in the middle stage of fermentation, culturing for 340 h, heating and inactivating, and filtering the strain with plate-and-frame filter pressSeparating mycelium from fermentation broth, adding calcium oxide into mycelium, granulating, drying, and extracting with acetone;
b. extracting with acetone to obtain griseofulvin acetone extractive solution;
c. decoloring the griseofulvin acetone extract by using activated carbon, and concentrating and crystallizing the decolored solution to obtain a griseofulvin crude product;
d. dissolving the griseofulvin crude product with acetone, decoloring the griseofulvin acetone solution with activated carbon, and concentrating and crystallizing the decolored solution to obtain griseofulvin recrystallization;
e. washing griseofulvin recrystallization with butanol, washing with ethanol, drying, and micronizing to obtain griseofulvin raw material.
Comparative experiment:
test formulations (culture Medium used in the invention)
6% of indica rice flour, 5% of corn flour, 3% of sweet potato flour, 0.6% of potassium dihydrogen phosphate, 0.4% of calcium carbonate, 0.1% of sodium nitrate, 0.1% of ammonium sulfate, 0.1% of ferrous sulfate, 0.8% of sodium chloride, 0.7% of potassium chloride, 0.1% of magnesium sulfate, 0.05% of a GPE (meglumine) type, and the balance of water.
Comparative formulation (Medium previously used by this company)
15% of indica rice flour, 0.4% of potassium dihydrogen phosphate, 0.6% of calcium carbonate, 0.1% of sodium nitrate, 0.1% of ammonium sulfate, 0.1% of ferrous sulfate, 0.8% of sodium chloride, 0.7% of potassium chloride, 0.1% of magnesium sulfate and the balance of water.
Mixing the above two formulas, sterilizing, culturing seed culture fluid in a fermentation tank, culturing after middle-stage feeding, inactivating, separating mycelium from fermentation broth, granulating, extracting, decolorizing, crystallizing, washing, drying, and micronizing to obtain griseofulvin raw material. The results are compared by three tests as shown in the table:
table 1 comparative test table
And (4) test conclusion: compared with a comparative formula, the experimental formula has the advantages that the filtration rate of the fermentation liquor in the experimental formula is obviously improved, the filtration yield is about 16 percent higher than that of the comparative formula, the pH value after the fermentation is reduced from 6.8 to 5.8, the fermentation titer is stabilized at 28000-30000 mu g/ml, the fermentation period is shortened by about 45 hours, 150 ten thousand yuan is saved every year, the efficiency and the yield of an enterprise are improved, and the economic benefit is about 1000 ten thousand.
Claims (1)
1. The fermentation production culture medium for griseofulvin is characterized by being prepared from the following raw materials in percentage by weight: 6% of long-shaped rice flour, 5% of corn flour, 3% of sweet potato flour, 0.6% of potassium dihydrogen phosphate, 0.4% of calcium carbonate, 0.1% of sodium nitrate, 0.1% of ammonium sulfate, 0.1% of ferrous sulfate, 0.8% of sodium chloride, 0.7% of potassium chloride, 0.1% of magnesium sulfate, 0.05% of kyoto GPE (glutathione) type, and the balance of water.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201910648868.5A CN110317841B (en) | 2019-07-10 | 2019-07-10 | Fermentation production culture medium for griseofulvin |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201910648868.5A CN110317841B (en) | 2019-07-10 | 2019-07-10 | Fermentation production culture medium for griseofulvin |
Publications (2)
Publication Number | Publication Date |
---|---|
CN110317841A CN110317841A (en) | 2019-10-11 |
CN110317841B true CN110317841B (en) | 2022-11-22 |
Family
ID=68123919
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201910648868.5A Active CN110317841B (en) | 2019-07-10 | 2019-07-10 | Fermentation production culture medium for griseofulvin |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN110317841B (en) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN112094874A (en) * | 2020-09-16 | 2020-12-18 | 内蒙古格林特制药有限责任公司 | Culture medium for producing griseofulvin through fermentation |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101812489A (en) * | 2010-04-29 | 2010-08-25 | 赤峰制药股份有限公司 | Culture medium for producing griseofulvin by fermentation |
CN103275047A (en) * | 2013-05-23 | 2013-09-04 | 内蒙古格林特制药有限责任公司 | Preparation method of griseofulvin |
CN106480121A (en) * | 2016-09-27 | 2017-03-08 | 宁夏泰瑞制药股份有限公司 | A kind of culture medium of utilization penicillium patulum producing griseofulvin by fermentation and cultural method |
CN108641969A (en) * | 2018-06-21 | 2018-10-12 | 赤峰制药股份有限公司 | A kind of griseofulvin production bacterium culture medium |
-
2019
- 2019-07-10 CN CN201910648868.5A patent/CN110317841B/en active Active
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101812489A (en) * | 2010-04-29 | 2010-08-25 | 赤峰制药股份有限公司 | Culture medium for producing griseofulvin by fermentation |
CN103275047A (en) * | 2013-05-23 | 2013-09-04 | 内蒙古格林特制药有限责任公司 | Preparation method of griseofulvin |
CN106480121A (en) * | 2016-09-27 | 2017-03-08 | 宁夏泰瑞制药股份有限公司 | A kind of culture medium of utilization penicillium patulum producing griseofulvin by fermentation and cultural method |
CN108641969A (en) * | 2018-06-21 | 2018-10-12 | 赤峰制药股份有限公司 | A kind of griseofulvin production bacterium culture medium |
Also Published As
Publication number | Publication date |
---|---|
CN110317841A (en) | 2019-10-11 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US9290787B2 (en) | Method of producing natural β-carotene by fermentation and use thereof | |
CN110317841B (en) | Fermentation production culture medium for griseofulvin | |
AU2015374763A1 (en) | Method for preparing Sofosbuvir crystal form-6 | |
US4339536A (en) | Process for the preparation of long-chain dicarboxylic acids by fermentation | |
CN107058410A (en) | A kind of preparation method of S abscisic acids | |
CN102643255A (en) | Andrographolide compound | |
CN112778150A (en) | Novel crystal form of gamma-aminobutyric acid and preparation method thereof | |
CN109266578B (en) | Escherichia coli ACThr1032 and application thereof in fermentation production of L-threonine | |
CN106609288B (en) | Method for improving industrial abamectin B by optimizing fermentation medium1aMethod of production of | |
US20030138920A1 (en) | Process for the isolation of polyhydroxy cyclic carboxylic acids | |
CN106591401B (en) | Fermentation promoter for increasing yield of gentamicin C1a and addition method thereof | |
CN115505622A (en) | Method for preparing UDCA isomer of 3 alpha, 7 beta-dihydroxy-5 alpha-H | |
CN115637281A (en) | Method for preparing UDCA isomer of 3 beta, 7 beta-dihydroxy-5 alpha-H | |
CN109096129A (en) | A kind of preparation method of L-carnitine-L-tartrate | |
CN115247193A (en) | Industrial production method of uridine | |
CN114621892A (en) | Escherichia coli with high polysialic acid yield and application thereof | |
CN106883286B (en) | Extraction and purification method of tyrosine derivative | |
TW200303363A (en) | Process to prepare and isolate geldanamycin | |
CN108794502B (en) | Trichothecene compound and preparation method and application thereof | |
WO2020263519A1 (en) | Method for the production of pullulan soft capsules | |
JP2873894B2 (en) | Cyclic depsipeptide and method for producing the same | |
CN1230552C (en) | Kojic acid producing process | |
CN107988305B (en) | Preparation method of gibberellic acid | |
CN113929615B (en) | Method for purifying nojirimycin | |
CN109206310A (en) | A method of extracting D-ALPHA-Hydroxypropionic acid from D-ALPHA-Hydroxypropionic acid calcium fermentation liquid |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant | ||
PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: A culture medium for the fermentation production of griseofulvin Granted publication date: 20221122 Pledgee: Huaxia Bank Co.,Ltd. Chifeng Branch Pledgor: INNER MONGOLIA GLINT PHARMACEUTICAL CO.,LTD. Registration number: Y2024150000049 |