CN109206310A - A method of extracting D-ALPHA-Hydroxypropionic acid from D-ALPHA-Hydroxypropionic acid calcium fermentation liquid - Google Patents
A method of extracting D-ALPHA-Hydroxypropionic acid from D-ALPHA-Hydroxypropionic acid calcium fermentation liquid Download PDFInfo
- Publication number
- CN109206310A CN109206310A CN201710534417.XA CN201710534417A CN109206310A CN 109206310 A CN109206310 A CN 109206310A CN 201710534417 A CN201710534417 A CN 201710534417A CN 109206310 A CN109206310 A CN 109206310A
- Authority
- CN
- China
- Prior art keywords
- alpha
- hydroxypropionic acid
- fermentation
- liquid
- calcium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C51/00—Preparation of carboxylic acids or their salts, halides or anhydrides
- C07C51/02—Preparation of carboxylic acids or their salts, halides or anhydrides from salts of carboxylic acids
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C51/00—Preparation of carboxylic acids or their salts, halides or anhydrides
- C07C51/42—Separation; Purification; Stabilisation; Use of additives
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C51/00—Preparation of carboxylic acids or their salts, halides or anhydrides
- C07C51/42—Separation; Purification; Stabilisation; Use of additives
- C07C51/47—Separation; Purification; Stabilisation; Use of additives by solid-liquid treatment; by chemisorption
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
- C12P7/56—Lactic acid
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07B—GENERAL METHODS OF ORGANIC CHEMISTRY; APPARATUS THEREFOR
- C07B2200/00—Indexing scheme relating to specific properties of organic compounds
- C07B2200/07—Optical isomers
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biotechnology (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The method that the invention discloses a kind of to extract D-ALPHA-Hydroxypropionic acid from D-ALPHA-Hydroxypropionic acid calcium fermentation liquid, including the following steps being connected in order: 1) carrying out being separated by solid-liquid separation removal thallus for D-ALPHA-Hydroxypropionic acid calcium fermentation liquid, obtain clarified broth；2) concentrated sulfuric acid is added in clarified broth obtained in step 1) and carries out acidolysis, then remove byproduct calcium sulfate；3) the D-ALPHA-Hydroxypropionic acid clear liquid of removal calcium sulfate obtained in step 2) activated-charcoal column is crossed to decolourize；4) D-ALPHA-Hydroxypropionic acid destainer obtained in step 3) is subjected to ion exchange；5) feed liquid obtained in step 4) is subjected to nanofiltration decoloration；6) fermentation liquid after nanofiltration obtained in step 5) is subjected to concentration to obtain D-ALPHA-Hydroxypropionic acid.The present invention produces bacterium by efficient D-ALPHA-Hydroxypropionic acid, obtains the D-ALPHA-Hydroxypropionic acid calcium of high concentration and high-optical-purity, and the fermentation medium components used are relatively simple, are easy to the separation and Extraction in downstream；It is decolourized using nanofiltration operation to fermentation liquid, ensure that the coloration of D-ALPHA-Hydroxypropionic acid product.
Description
Technical field
The method that the present invention relates to a kind of to extract D-ALPHA-Hydroxypropionic acid from D-ALPHA-Hydroxypropionic acid calcium fermentation liquid, belongs to bioengineering field.
Background technique
Lactic acid is one of big organic acid in the world three, it can be widely used in the fields such as food, medicine, chemical industry and agricultural.
Special D-ALPHA-Hydroxypropionic acid can be widely used in medicine, chemical industry and agriculture field.The polylactic acid synthesized using D-ALPHA-Hydroxypropionic acid as monomer with
Its good biodegradable properties and other excellent use characteristics (such as transparency, thermoplasticity, Product safety) have become
For the green material that 21 century is most with prospects.Furthermore the biological pesticide " galloping horse " that is manufactured with high-purity D-ALPHA-Hydroxypropionic acid and
Herbicides such as " prestige despots ", as novel hypotoxicity pesticide, can effective herbicide pesticide it is both economical and reliable.In the market, together
5~10 times more expensive than Pfansteihl of the D-ALPHA-Hydroxypropionic acid price of grade.Therefore the production and purifying of D-ALPHA-Hydroxypropionic acid are gradually taken seriously.
The preparation method of lactic acid has fermentation method, chemical synthesis and enzyme process.Wherein, fermentation method and chemical synthesis have obtained
Industrial application, and China mainly uses fermentation method.
Traditional lactic acid extraction process is calcium lactate crystallization-acidolysis process, and the technical maturity is easily controllable, but there are works
The disadvantages of skill route is long, and unit operation is more, and waste liquor contamination is serious, and energy consumption is higher, and entire separation process the degree of automation is lower, it is newborn
Acid recovering rate is also generally between 40~50%, and the product quality obtained is lower.
Summary of the invention
In order to solve to prepare the defects of complicated, product yield is lower, second-rate in the prior art, the present invention provides one kind
The method of D-ALPHA-Hydroxypropionic acid is extracted from D-ALPHA-Hydroxypropionic acid calcium fermentation liquid.
In order to solve the above technical problems, the technical solution adopted in the present invention is as follows:
A method of extracting D-ALPHA-Hydroxypropionic acid from D-ALPHA-Hydroxypropionic acid calcium fermentation liquid, including the following steps being connected in order:
1) D-ALPHA-Hydroxypropionic acid calcium fermentation liquid is carried out being separated by solid-liquid separation removal thallus, obtains clarified broth；
2) concentrated sulfuric acid is added in clarified broth obtained in step 1) and carries out acidolysis, then remove by-product sulfuric acid
Calcium；
3) the D-ALPHA-Hydroxypropionic acid clear liquid of removal calcium sulfate obtained in step 2) activated-charcoal column is crossed to decolourize；
4) D-ALPHA-Hydroxypropionic acid destainer obtained in step 3) is subjected to ion exchange；
5) feed liquid obtained in step 4) is subjected to nanofiltration decoloration；
6) fermentation liquid after nanofiltration obtained in step 5) is subjected to concentration to obtain D-ALPHA-Hydroxypropionic acid.
The concentrated sulfuric acid in the application refers to that mass concentration is 98% sulfuric acid.
In order to further increase product quality, in step 1), the separation of solid and liquid of fermentation liquid is using centrifugation or plate-frame filtering
Mode.
In order to simplify operation, while guaranteeing product quality, in step 2), 55~75 DEG C of acidolysis temperature, the acidolysis time 1~
4h, calcium sulfate removal is by the way of centrifugation or plate-frame filtering.
The concentrated sulfuric acid in the application refers to that mass concentration is 98% sulfuric acid.
In order to further increase product quality, in step 3), active carbon pillar uses granular activated carbon, operation temperature 50
~70 DEG C.
In order to further increase product quality, in step 4), ion exchange uses resin cation and resin anion (R.A.) phase
In conjunction with method carry out.
In order to further increase product quality, in step 4), fermentation liquid first crosses cation seperation column after anion column, cation
Exchanger resin is 732 resin cations, and resin anion (R.A.) is 717 resin anion (R.A.)s.
In order to further increase product quality, in step 5), decolourized using nanofiltration membrane, the molecular cut off of nanofiltration membrane
For 200~500D.
In order to further increase product quality, in step 1), D-ALPHA-Hydroxypropionic acid calcium fermentation liquid is obtained using microbe fermentation method.
Fermenting microbe of the present invention is that the D-ALPHA-Hydroxypropionic acid of a height growth rate of producing acid produces bacterium, in December, 2012
It is preserved within 6th China typical culture collection center (China, Wuhan, Wuhan University), classification naming is lactobacillus
(Sporolactobacillussp.) BS1-5, preservation registration number are CCTCC NO.M2012516, are more specifically classified as synanthrin
Lactobacillus (Sporolactobacillussp.inulinus).
In step 1), the preparation method of D-ALPHA-Hydroxypropionic acid calcium fermentation liquid includes the following steps being connected in order:
(1) plate culture: being seeded to plating medium Anaerobic culturel for bacillus BS1-5, and 30~45 DEG C of cultivation temperature,
20~48h of incubation time；
(2) seed culture: being seeded to seed culture medium Anaerobic culturel for the bacillus through step (1) plate culture, training
Feeding 30~45 DEG C of temperature, incubation time 12~for 24 hours；
(3) fermentation and acid: the seed culture fluid that step (2) obtain being seeded in fermentation medium and carries out fermentation and acid,
Inoculum concentration is 3%~15%, 30~45 DEG C of fermentation temperature, is passed through the environment that nitrogen keeps its anaerobism, will be fermented using neutralizer
The pH of system is controlled 6.0~7.0.
In order to further increase product recovery rate, plating medium ingredient is (g/L): glucose 10~30, yeast extract 1~
3, anhydrous sodium acetate 1~4, anhydrous magnesium sulfate 0.1~0.4, potassium dihydrogen phosphate 1~3, agar 15~25；Seed culture based component
For (g/L): glucose 10~40, yeast extract 1~3, peptone 1~3, anhydrous magnesium sulfate 0.1~0.4, calcium carbonate 10~30；Hair
Ferment medium component is (g/L): glucose 150~180, yeast extract 8~12, Dried Corn Steep Liquor Powder 4~6, magnesium sulfate 0.4~0.6.
The unmentioned technology of the present invention is referring to the prior art.
The present invention has the beneficial effect that:
(1) present invention produces bacterium by efficient D-ALPHA-Hydroxypropionic acid, obtains the D-ALPHA-Hydroxypropionic acid calcium of high concentration and high-optical-purity, and
The fermentation medium components of use are relatively simple, are easy to the separation and Extraction in downstream；
(2) present invention decolourizes to fermentation liquid using nanofiltration operation, ensure that the coloration of D-ALPHA-Hydroxypropionic acid product；
(3) the entire process flow of the present invention has accomplished operation most on the basis of the D-ALPHA-Hydroxypropionic acid for being able to achieve high-quality separates
Optimization reduces the expense isolated and purified in fermentation method production D-ALPHA-Hydroxypropionic acid, and separation yield is up to 85%.
Specific embodiment
For a better understanding of the present invention, below with reference to the embodiment content that the present invention is furture elucidated, but it is of the invention
Content is not limited solely to the following examples.
Embodiment 1:
(1) plate culture: bacillus BS1-5 is seeded to plating medium Anaerobic culturel, 30 DEG C of cultivation temperature, is cultivated
Time 48h；
(2) seed culture: being seeded to seed culture medium Anaerobic culturel for the bacillus through step (1) plate culture, training
30 DEG C of temperature is supported, incubation time is for 24 hours；
(3) fermentation and acid: the seed culture fluid that step (2) obtain being seeded in fermentation medium and carries out fermentation and acid,
Inoculum concentration is 15%, 30 DEG C of fermentation temperature, is passed through the environment that nitrogen keeps its anaerobism, is controlled the pH of fermentation system using neutralizer
System is 6.0.
Above-mentioned plating medium ingredient be (g/L): glucose 10, yeast extract 1, anhydrous sodium acetate 1, anhydrous magnesium sulfate 0.1,
Potassium dihydrogen phosphate 1, agar 15.
Above-mentioned seed culture based component is (g/L): glucose 10, yeast extract 1, peptone 1, anhydrous magnesium sulfate 0.1, carbonic acid
Calcium 10.
Above-mentioned fermentation medium components are (g/L): glucose 150, yeast extract 8, Dried Corn Steep Liquor Powder 4, magnesium sulfate 0.4.
The fermentation and acid stage is fermented using calcium carbonate as pH adjusting agent with the D-ALPHA-Hydroxypropionic acid calcium of Bacillus production strain
Liquid, wherein D-ALPHA-Hydroxypropionic acid concentration 115g/L, no residual sugar.D-ALPHA-Hydroxypropionic acid calcium fermentation liquid after fermentation is taken to carry out solid-liquid point through sheet frame
From by the addition concentrated sulfuric acid progress acidolysis of filtered clear liquid, acidolysis temperature is controlled at 55 DEG C, acidolysis time 1h, then uses plate
Frame filtering removal byproduct calcium sulfate；The clear liquid of acquisition is sent into activated-charcoal column and carries out decolorization operations；By the fermentation liquid after decoloration
Ion exchange is carried out, ion exchange selects 732 resin cations and 717 anion exchange resin, first crosses cation seperation column after yin
Ion column；Fermentation liquid after ion exchange is subjected to nanofiltration decoloration, the molecular cut off of nanofiltration membrane is 500D, is finally sent into multiple-effect
Evaporator, which is evaporated under reduced pressure, obtains high-quality D-ALPHA-Hydroxypropionic acid, and obtained D-ALPHA-Hydroxypropionic acid content is 89.5%, and optical purity is
99.5%.
Embodiment 2:
(1) plate culture: bacillus BS1-5 is seeded to plating medium Anaerobic culturel, 45 DEG C of cultivation temperature, is cultivated
Time 20h；
(2) seed culture: being seeded to seed culture medium Anaerobic culturel for the bacillus through step (1) plate culture, training
Support temperature 45 C, incubation time 12h；
(3) fermentation and acid: the seed culture fluid that step (2) obtain being seeded in fermentation medium and carries out fermentation and acid,
Inoculum concentration is 3%, 45 DEG C of fermentation temperature, is passed through the environment that nitrogen keeps its anaerobism, is controlled the pH of fermentation system using neutralizer
System is 7.0.
Above-mentioned plating medium ingredient be (g/L): glucose 30, yeast extract 3, anhydrous sodium acetate 4, anhydrous magnesium sulfate 0.4,
Potassium dihydrogen phosphate 3, agar 25.
Above-mentioned seed culture based component is (g/L): glucose 40, yeast extract 3, peptone 3, anhydrous magnesium sulfate 0.4, carbonic acid
Calcium 30.
Above-mentioned fermentation medium components are (g/L): glucose 180, yeast extract 12, Dried Corn Steep Liquor Powder 6, magnesium sulfate 0.6.
The fermentation and acid stage is fermented using calcium hydroxide as pH adjusting agent with the D-ALPHA-Hydroxypropionic acid calcium of Bacillus production strain
Liquid, wherein D-ALPHA-Hydroxypropionic acid concentration 130g/L, no residual sugar.D-ALPHA-Hydroxypropionic acid calcium fermentation liquid after fermentation is taken to carry out solid-liquid point through sheet frame
From by the addition concentrated sulfuric acid progress acidolysis of filtered clear liquid, acidolysis temperature is controlled at 75 DEG C, acidolysis time 4h, then uses plate
Frame filtering removal byproduct calcium sulfate；The clear liquid of acquisition is sent into activated-charcoal column and carries out decolorization operations；By the fermentation liquid after decoloration
Ion exchange is carried out, ion exchange selects 732 resin cations and 717 anion exchange resin, first crosses cation seperation column after yin
Ion column；Fermentation liquid after ion exchange is subjected to nanofiltration decoloration, the molecular cut off of nanofiltration membrane is 200D, is finally sent into multiple-effect
Evaporator, which is evaporated under reduced pressure, obtains high-quality D-ALPHA-Hydroxypropionic acid, and obtained D-ALPHA-Hydroxypropionic acid content is 89%, optical purity 99.5%.
Embodiment 3:
(1) plate culture: bacillus BS1-5 is seeded to plating medium Anaerobic culturel, 37 DEG C of cultivation temperature, is cultivated
Time is for 24 hours；
(2) seed culture: being seeded to seed culture medium Anaerobic culturel for the bacillus through step (1) plate culture,
37 DEG C of cultivation temperature, incubation time 20h；
(3) fermentation and acid: the seed culture fluid that step (2) obtain being seeded in fermentation medium and carries out fermentation and acid,
Inoculum concentration is 10%, 37 DEG C of fermentation temperature, is passed through the environment that nitrogen keeps its anaerobism, is controlled the pH of fermentation system using neutralizer
System is 6.5.
Above-mentioned plating medium ingredient be (g/L): glucose 20, yeast extract 2, anhydrous sodium acetate 2, anhydrous magnesium sulfate 0.3,
Potassium dihydrogen phosphate 2, agar 20.
Above-mentioned seed culture based component is (g/L): glucose 20, yeast extract 2, peptone 2, anhydrous magnesium sulfate 0.3, carbonic acid
Calcium 20.
Above-mentioned fermentation medium components are (g/L): glucose 160, yeast extract 10, Dried Corn Steep Liquor Powder 5, magnesium sulfate 0.5.
The fermentation and acid stage is fermented using calcium carbonate as pH adjusting agent with the D-ALPHA-Hydroxypropionic acid calcium of Bacillus production strain
Liquid, wherein D-ALPHA-Hydroxypropionic acid concentration 144g/L, no residual sugar.D-ALPHA-Hydroxypropionic acid calcium fermentation liquid after fermentation is taken to carry out solid-liquid point through sheet frame
From by the addition concentrated sulfuric acid progress acidolysis of filtered clear liquid, acidolysis temperature is controlled at 65 DEG C, acidolysis time 2h, then uses plate
Frame filtering removal byproduct calcium sulfate；The clear liquid of acquisition is sent into activated-charcoal column and carries out decolorization operations；By the fermentation liquid after decoloration
Ion exchange is carried out, ion exchange selects 732 resin cations and 717 anion exchange resin, first crosses cation seperation column after yin
Ion column；Fermentation liquid after ion exchange is subjected to nanofiltration decoloration, the molecular cut off of nanofiltration membrane is 300, is finally sent into multiple-effect
Evaporator, which is evaporated under reduced pressure, obtains high-quality D-ALPHA-Hydroxypropionic acid, and obtained D-ALPHA-Hydroxypropionic acid content is 91%, optical purity 99.7%.
Embodiment 4:
(1) plate culture: bacillus BS1-5 is seeded to plating medium Anaerobic culturel, 37 DEG C of cultivation temperature, is cultivated
Time is for 24 hours；
(2) seed culture: being seeded to seed culture medium Anaerobic culturel for the bacillus through step (1) plate culture,
37 DEG C of cultivation temperature, incubation time 20h；
(3) fermentation and acid: the seed culture fluid that step (2) obtain being seeded in fermentation medium and carries out fermentation and acid,
Inoculum concentration is 10%, 37 DEG C of fermentation temperature, is passed through the environment that nitrogen keeps its anaerobism, is controlled the pH of fermentation system using neutralizer
System is 6.5.
Above-mentioned plating medium ingredient be (g/L): glucose 20, yeast extract 2, anhydrous sodium acetate 2, anhydrous magnesium sulfate 0.3,
Potassium dihydrogen phosphate 2, agar 20.
Above-mentioned seed culture based component is (g/L): glucose 20, yeast extract 2, peptone 2, anhydrous magnesium sulfate 0.3, carbonic acid
Calcium 20.
Above-mentioned fermentation medium components are (g/L): glucose 150, yeast extract 10, Dried Corn Steep Liquor Powder 5, magnesium sulfate 0.5, bran
Skin 10.
The fermentation and acid stage is fermented using calcium carbonate as pH adjusting agent with the D-ALPHA-Hydroxypropionic acid calcium of Bacillus production strain
Liquid, wherein D-ALPHA-Hydroxypropionic acid concentration 135g/L, no residual sugar.With 5L fermentation liquid, concentration 135g/L, there are about the D-ALPHA-Hydroxypropionic acids of 675g mass.
5L is taken, the fermentation liquid of the D-ALPHA-Hydroxypropionic acid containing 675g is separated by solid-liquid separation through sheet frame, and D-ALPHA-Hydroxypropionic acid yield is 98%, sheet frame mistake
The quality that D-ALPHA-Hydroxypropionic acid is obtained after filter is 661g, and the concentrated sulfuric acid is added in filtered clear liquid and carries out acidolysis.Acidolysis temperature is controlled 65
DEG C, acidolysis time 2h then removes byproduct calcium sulfate using plate-frame filtering, obtains 490g byproduct calcium sulfate altogether；It will obtain
Clear liquid be sent into activated-charcoal column carry out decolorization operations；Fermentation liquid after decoloration is subjected to ion exchange, ion exchange selects 732 sun
Ion exchange resin and 717 anion exchange resin first cross cation seperation column after anion column；Fermentation liquid after ion exchange is carried out
Nanofiltration decoloration, the molecular cut off of nanofiltration membrane are 200D, and finally feeding multi-effect evaporator, which is evaporated under reduced pressure, obtains high-quality
D-ALPHA-Hydroxypropionic acid, obtained D-ALPHA-Hydroxypropionic acid content are 90%, and optical purity 99.7%, obtaining D-ALPHA-Hydroxypropionic acid product quality altogether is 573g.
Finally the total recovery of separation and Extraction D-ALPHA-Hydroxypropionic acid product is 85% from containing D-ALPHA-Hydroxypropionic acid calcium fermentation liquid.
Claims (10)
1. a kind of method for extracting D-ALPHA-Hydroxypropionic acid from D-ALPHA-Hydroxypropionic acid calcium fermentation liquid, it is characterised in that: including the following step being connected in order
It is rapid:
1) D-ALPHA-Hydroxypropionic acid calcium fermentation liquid is carried out being separated by solid-liquid separation removal thallus, obtains clarified broth；
2) concentrated sulfuric acid is added in clarified broth obtained in step 1) and carries out acidolysis, then remove byproduct calcium sulfate；
3) the D-ALPHA-Hydroxypropionic acid clear liquid of removal calcium sulfate obtained in step 2) activated-charcoal column is crossed to decolourize；
4) D-ALPHA-Hydroxypropionic acid destainer obtained in step 3) is subjected to ion exchange；
5) feed liquid obtained in step 4) is subjected to nanofiltration decoloration；
6) fermentation liquid after nanofiltration obtained in step 5) is subjected to concentration to obtain D-ALPHA-Hydroxypropionic acid.
2. the method as described in claim 1, it is characterised in that: in step 1), the separation of solid and liquid of fermentation liquid using centrifugation or
The mode of plate-frame filtering.
3. the method as described in claim 1, it is characterised in that: in step 2), 55~75 DEG C of acidolysis temperature, the acidolysis time 1~
4h, calcium sulfate removal is by the way of centrifugation or plate-frame filtering.
4. the method as described in claim 1, it is characterised in that: in step 3), active carbon pillar uses granular activated carbon, operation
Temperature is 50~70 DEG C.
5. the method as described in claim 1, it is characterised in that: in step 4), ion exchange using resin cation and yin from
The method that subtree lipid phase combines carries out.
6. method as claimed in claim 5, it is characterised in that: in step 4), fermentation liquid first crosses cation seperation column after anion
Column, cation exchange resin are 732 resin cations, and resin anion (R.A.) is 717 resin anion (R.A.)s.
7. the method as described in claim 1, it is characterised in that: in step 5), decolourized using nanofiltration membrane, nanofiltration membrane is cut
Staying molecular weight is 200~500D.
8. the method as described in claim 1, it is characterised in that: in step 1), D-ALPHA-Hydroxypropionic acid calcium fermentation liquid uses microbial fermentation
Method obtains.
9. method according to claim 8, it is characterised in that: in step 1), the preparation method of D-ALPHA-Hydroxypropionic acid calcium fermentation liquid includes
The following steps being connected in order:
(1) plate culture: bacillus BS1-5 is seeded to plating medium Anaerobic culturel, 30~45 DEG C of cultivation temperature, is cultivated
20~48h of time；
(2) bacillus through step (1) plate culture seed culture: is seeded to seed culture medium Anaerobic culturel, culture temperature
30~45 DEG C of degree, incubation time 12~for 24 hours；
(3) fermentation and acid: the seed culture fluid that step (2) obtain being seeded in fermentation medium and carries out fermentation and acid, is inoculated with
Amount is 3%~15%, 30~45 DEG C of fermentation temperature, is passed through the environment that nitrogen keeps its anaerobism, using neutralizer by fermentation system
PH control 6.0~7.0.
10. method as claimed in claim 9, it is characterised in that: plating medium ingredient is (g/L): glucose 10~30, ferment
Female cream 1~3, anhydrous sodium acetate 1~4, anhydrous magnesium sulfate 0.1~0.4, potassium dihydrogen phosphate 1~3, agar 15~25；Seed culture
Based component is (g/L): glucose 10~40, yeast extract 1~3, peptone 1~3, anhydrous magnesium sulfate 0.1~0.4, calcium carbonate 10
~30；Fermentation medium components are (g/L): glucose 150~180, yeast extract 8~12, Dried Corn Steep Liquor Powder 4~6, magnesium sulfate
0.4~0.6.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710534417.XA CN109206310A (en) | 2017-07-03 | 2017-07-03 | A method of extracting D-ALPHA-Hydroxypropionic acid from D-ALPHA-Hydroxypropionic acid calcium fermentation liquid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710534417.XA CN109206310A (en) | 2017-07-03 | 2017-07-03 | A method of extracting D-ALPHA-Hydroxypropionic acid from D-ALPHA-Hydroxypropionic acid calcium fermentation liquid |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109206310A true CN109206310A (en) | 2019-01-15 |
Family
ID=64992470
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710534417.XA Pending CN109206310A (en) | 2017-07-03 | 2017-07-03 | A method of extracting D-ALPHA-Hydroxypropionic acid from D-ALPHA-Hydroxypropionic acid calcium fermentation liquid |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109206310A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111269107A (en) * | 2020-04-09 | 2020-06-12 | 安徽固德生物工程有限公司 | L-lactic acid purification and refining method |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20020004610A1 (en) * | 2000-05-23 | 2002-01-10 | Eric Dubois | Process for the preparation of lactic acid by evaporative crystallisation |
| CN1840675A (en) * | 2006-02-08 | 2006-10-04 | 沈阳农业大学 | Process for producing high activity soybean oligosaccharide by lactic acid bacteria fermentation method |
| CN101306993A (en) * | 2007-05-14 | 2008-11-19 | 张家港三源生物工程有限公司 | Refine process of L-lactic acid of polymerization grade |
| CN101736042A (en) * | 2010-01-08 | 2010-06-16 | 合肥工业大学 | Method for producing L-lactic acid |
| CN101967091A (en) * | 2009-07-28 | 2011-02-09 | 凯发知识产权资源私人有限公司 | Method or purifying organic acid |
| US20130245320A1 (en) * | 2010-08-19 | 2013-09-19 | Companhia Refinadora Da Amazonia | Method for obtaining lactic acid with a high degree of purity from fermentative liquor |
| CN104080833A (en) * | 2011-11-04 | 2014-10-01 | 蒂森克虏伯工业解决方案股份公司 | Quality test for polymerizable lactic acid and method for producing same |
| CN104419657A (en) * | 2013-09-11 | 2015-03-18 | 中国石油化工股份有限公司 | D-lactic acid producing strain with high growth rate and acid producing velocity and application thereof |
| CN104974032A (en) * | 2014-04-14 | 2015-10-14 | 中国石化扬子石油化工有限公司 | Method of separation and extraction of D-lactic acid from sodium D-lactate fermentation liquid |
| CN106316832A (en) * | 2015-07-02 | 2017-01-11 | 中国石化扬子石油化工有限公司 | Method for obtaining high-purity lactic acid by separating non-calcium salt lactic acid fermentation broth |
-
2017
- 2017-07-03 CN CN201710534417.XA patent/CN109206310A/en active Pending
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20020004610A1 (en) * | 2000-05-23 | 2002-01-10 | Eric Dubois | Process for the preparation of lactic acid by evaporative crystallisation |
| CN1840675A (en) * | 2006-02-08 | 2006-10-04 | 沈阳农业大学 | Process for producing high activity soybean oligosaccharide by lactic acid bacteria fermentation method |
| CN101306993A (en) * | 2007-05-14 | 2008-11-19 | 张家港三源生物工程有限公司 | Refine process of L-lactic acid of polymerization grade |
| CN101967091A (en) * | 2009-07-28 | 2011-02-09 | 凯发知识产权资源私人有限公司 | Method or purifying organic acid |
| CN101736042A (en) * | 2010-01-08 | 2010-06-16 | 合肥工业大学 | Method for producing L-lactic acid |
| US20130245320A1 (en) * | 2010-08-19 | 2013-09-19 | Companhia Refinadora Da Amazonia | Method for obtaining lactic acid with a high degree of purity from fermentative liquor |
| CN104080833A (en) * | 2011-11-04 | 2014-10-01 | 蒂森克虏伯工业解决方案股份公司 | Quality test for polymerizable lactic acid and method for producing same |
| CN104419657A (en) * | 2013-09-11 | 2015-03-18 | 中国石油化工股份有限公司 | D-lactic acid producing strain with high growth rate and acid producing velocity and application thereof |
| CN104974032A (en) * | 2014-04-14 | 2015-10-14 | 中国石化扬子石油化工有限公司 | Method of separation and extraction of D-lactic acid from sodium D-lactate fermentation liquid |
| CN106316832A (en) * | 2015-07-02 | 2017-01-11 | 中国石化扬子石油化工有限公司 | Method for obtaining high-purity lactic acid by separating non-calcium salt lactic acid fermentation broth |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111269107A (en) * | 2020-04-09 | 2020-06-12 | 安徽固德生物工程有限公司 | L-lactic acid purification and refining method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Berovic et al. | Citric acid production | |
| CN110035991A (en) | One-step fermentation prepares (R) -3-hydroxybutyrate or its salt | |
| CN107058416B (en) | Fermentation process for refining glutamic acid | |
| CN109161492A (en) | A kind of gamma-polyglutamic acid generating bacterium and the method for efficiently synthesizing gamma-polyglutamic acid | |
| CN107418995B (en) | A kind of ellagic acid and preparation method thereof of granatanine liquid state fermentation preparation | |
| CN102399837B (en) | Method for synthesizing acarbose through microbial fermentation | |
| CN107083406A (en) | (R) production method of 3 hydroxybutyric acids | |
| CN109486876A (en) | A method of threonine is extracted and is purified in fermentation | |
| EP3332016A1 (en) | Improved production of vanillin by fermentation | |
| CN107201384A (en) | A kind of method of separation and Extraction D-ALPHA-Hydroxypropionic acid in sodium zymotic fluid from D-ALPHA-Hydroxypropionic acid | |
| CN105886573B (en) | Method for preparing trehalose by continuous extracellular enzyme biological method | |
| CN113321580B (en) | Method for producing malic acid | |
| CN104531810B (en) | A kind of method that high-effective microorganism conversion prepares maltobionic acid | |
| CN108285913B (en) | Process for preparing and extracting L-glutamine | |
| CN102703334B (en) | Strain producing erythritol and method for producing erythritol by using strain | |
| CN110862314A (en) | Method for separating and extracting D-lactic acid from D-sodium lactate fermentation broth | |
| CN102703525B (en) | Method for increasing yield of erythritol by adjusting osmotic pressure of fermentation liquor | |
| CN101475972B (en) | Method for producing L-arabinose from corncob | |
| CN108251476A (en) | The method that vitamin B12 is extracted from enzyme preparation waste water | |
| CN109206310A (en) | A method of extracting D-ALPHA-Hydroxypropionic acid from D-ALPHA-Hydroxypropionic acid calcium fermentation liquid | |
| CN105001303B (en) | A method of using cryogenic freezing environment from antibacterial lipopeptid solution desalination | |
| CN101857886B (en) | Method for preparing xylitol and co-producing L-arabinose | |
| CN109206312A (en) | A method of D-ALPHA-Hydroxypropionic acid is isolated and purified from D-ALPHA-Hydroxypropionic acid ammonium fermentation liquid | |
| CN113862179A (en) | Rhodopseudomonas palustris, application and method for preparing 5-ALA by using rhodopseudomonas palustris | |
| CN102321683B (en) | Process for preparing fumaric acid fermentation liquid by fermentation method and for separating and extracting pure fumaric acid from fumaric acid fermentation liquid |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190115 |
|
| RJ01 | Rejection of invention patent application after publication |