CN1230552C - Kojic acid producing process - Google Patents
Kojic acid producing process Download PDFInfo
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- CN1230552C CN1230552C CN 01135102 CN01135102A CN1230552C CN 1230552 C CN1230552 C CN 1230552C CN 01135102 CN01135102 CN 01135102 CN 01135102 A CN01135102 A CN 01135102A CN 1230552 C CN1230552 C CN 1230552C
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- kojic acid
- starch
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- saccharification
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Abstract
The present invention provides a production technology of kojic acid, which belongs to the preparing field of oxygenous organic compounds. The technology comprises starch saccharification, inoculation and fermentation, and kojic acid refining. The technology is characterized in that starch is directly put into a fermentation tank for liquefaction and saccharification; after being disinfected, the saccharide liquid is used for culturing kojic acid by inoculating fermentation; after the product obtained by the multiple concentration and crystallization of kojic acid clear liquid is dissolved and decolorized, the finished product of kojic acid crystals can be obtained by cooling crystallization and purification. In the method, the starch is directly put into the fermentation tank with an adding quantity of 5 to 15 mu/g starch of alpha-amylase; the temperature is raised to 70 to 100 DEG C for liquefaction and saccharification; the disinfecting temperature of the saccharide liquid is from 110 to 130 DEG C; the disinfecting time is from 30 to 40 minutes; the temperature of inoculating fermentation is from 32 to 36 DEG C; during dissolution and decolorization, the concentration of the dissolving liquid of the product obtained by multiple concentration and crystallization is from 9 to 12 Be'; 0.1 to 5 wt% of activated carbon is used as a decoloring agent. The acidity of the produced kojic acid is larger than 6%, the purity of the obtained kojic acid is more than 98%, and the crystals are uniform.
Description
Technical field
The invention belongs to the preparation of oxygen-containing organic compound, relate in particular to the preparation of ketone organic compound.
Background technology
Kojic acid formal name used at school 5-hydroxyl-2-methylol-1, pyrokomane, English name Kojic acid (5-hydroxy-2-hydroxamethy-1,4-pyrone).High-purity kojic acid is white or beige needle-like crystal; Soluble in water, pure, acetone are slightly soluble in ether, ethyl acetate, chloroform and pyridine, are insoluble to benzene; Its molecular formula is C
6H
6O
4, molecular weight is 142.1, fusing point is 153～156 ℃.It is a kind of important organic compound, has important purposes in food, the crystalline substance of making up, medicine and other fields, and it can remove the intravital free radical of people, strengthens cell viability, is a kind of product safe in utilization, broad-spectrum.
Kojic acid is the organic acid that utilizes fermentative Production, aspect the kojic acid synthetic technology, is raw material with starch how, by extracting refining obtaining behind the microbial fermentation.China is from the eighties kojic acid production technology that begins one's study, and carried out tackling of key scientific and technical problems for many years, but in 1997 dependence on import always in the past." Chinese chemical industry newspaper " is at report Shaanxi Institute of Microbiology employing microorganism complex mutation breeding technique on September 27 calendar year 2001.The flavus mutant strain UCN7-17 fermentation and acid that seed selection obtains can reach 6.3%, for the bacterial screening of the kojic acid production of China with realize that industrialization lays a good foundation.At present, domestic production technology for kojic acid also is in research and pilot scale stage, and the production technique imperfection of kojic acid: saccharification and purifying technique in the kojic acid production process adopt conventional art more, the many and complicated operation of production unit, and energy consumption is big, and the purity of kojic acid is low.
Summary of the invention
The invention provides a kind of perfect technology, simple to operate, acid production rate is high, purity is high kojic acid producing process.
The invention discloses a kind of kojic acid producing process: this technology is made up of mashing, inoculation fermentation and refining three steps, and it is characterized in that: starch directly drops into fermentor tank, and the amylase of adding is α-Dian Fenmei, and add-on is 5～15u/g starch; Be warming up to 70～100 ℃ of liquefaction and saccharification; Liquid glucose is through 110～130 ℃ of temperature, and the time is inoculated bacterial classification aspergillus oryzae Aspergillus oryzae after being sterilization in 30-40 minute, and the temperature of inoculation fermentation is 32～36 ℃, carries out ventilating fermentation, cultivates kojic acid;
The repeatedly condensing crystal product of kojic acid clear liquid is through dissolving, decolouring, when dissolving, decolouring repeatedly the concentration of the lysate of condensing crystal product be 9-12Be ', decrease temperature crystalline is purified and is obtained the kojic acid crystal of finished product.
Above-mentioned kojic acid producing process, the discoloring agent that adopts when it is characterized in that decolouring is the gac of 0.1-5% weight.
The acid production rate of this production technique＞6%, the purity of finished product kojic acid reaches more than 98%: transmittance is more than 95%, and crystal is even.
The invention has the advantages that the technical scale kojic acid producing process that provides perfect, process operations is simple; Shortened the production cycle, and ferment effect is good, production unit is few, and production cost is low; The acid production rate height, the product purity height, transmittance is good, and the kojic acid crystal is even.
Embodiment
Drop into 6 tons of W-Gums in embodiment 1 fermentor tank, add water and size mixing, drop into 15 kilograms of α-Dian Fenmei, be warming up to 100 ℃ indirectly and liquefy and saccharification.Detect liquefaction and saccharification situation with iodine reagent, when saccharification is red-brown to the constant blueness of iodine reagent, add the magnesium chloride nutritive salt of reasonable amount, directly be warming up to 130 ℃, be incubated 30 minutes and carry out disinfection.Be cooled to 36 ℃, insert aspergillus oryzae (Aspergillus oryzae), the cultivation and fermentation of ventilating, acid production rate is 6.7%, the fermented liquid color is little yellow.Fermented liquid after plate-and-frame filter press filters, is obtained the kojic acid clear liquid, clear liquid through condensing crystal and centrifugation several times, is obtained repeatedly crystallization crystal of kojic acid.Repeatedly the crystallization crystal drops into molten brilliant jar, adds softening water dissolving crystal, is warming up to about 85 ℃, and regulating lysate concentration is 12Be ', adds 5% gac and 0.2% organosilicon food defoamer, and being incubated 30 minutes decolours, and filters then.Filtrate enters crystallizer, is cooled to 10～12 ℃, and the centrifugation xln is dried, the kojic acid finished product that obtains, and crystal color is little yellow, and transmittance is 95%, and purity is 98.2%, and crystal is more even.
Embodiment 2 drops into 6 tons of W-Gums in fermentor tank, add entry and size mixing, and drops into 45 kilograms of α-Dian Fenmei, is warming up to 70 ℃ indirectly and liquefies and saccharification.Detect the saccharification situation, when saccharification is red-brown to the constant blueness of iodine reagent, add the Repone K nutritive salt of reasonable amount, directly be warming up to 110 ℃, be incubated 30 minutes and carry out disinfection.Be cooled to 32 ℃, insert aspergillus oryzae (Aspergillus oryzae), the cultivation and fermentation of ventilating, acid production rate is 6.5%, the fermented liquid color is little yellow.Fermented liquid after plate-and-frame filter press filters, is obtained the kojic acid clear liquid, clear liquid through condensing crystal and centrifugation several times, is obtained repeatedly crystallization crystal of kojic acid.Repeatedly crystallization crystal input is molten brilliant jar, adds softening water dissolving crystal, is warming up to about 85 ℃, and regulator solution concentration is 9Be ', and the gac and the 0.2% organosilicon food defoamer of adding 0.1% decolour, and are incubated 30 minutes and filter.Filtrate enters crystallizer, is cooled to 10～12 ℃, and the centrifugation xln is dried.Promptly obtain the kojic acid finished product, crystal color is little yellow, and transmittance is 95%, and purity is 98.0%, and crystal is more even.
Embodiment 3 repeats embodiment 1 by described same steps as, but the α-Dian Fenmei that drops into is 30 kilograms, starch liquefacation and saccharification temperature are 85 ℃, the liquid glucose sterilisation temp is 120 ℃, disinfecting time 35 minutes, inoculation fermentation temperature are 34 ℃, and acid production rate is 6.7%, crystal purity is 98.2%, and crystal is even.
Embodiment 4 repeats embodiment 1 by described same steps as, but the α-Dian Fenmei that drops into is 45 kilograms, and acid production rate is 6.5%, and the purity that obtains the kojic acid finished product is 98.0%, and crystal is even.
Embodiment 5 repeats embodiment 1 by described same steps as, but the α-Dian Fenmei that drops into is 45 kilograms, and the liquid glucose sterilisation temp is 110 ℃, is incubated 40 minutes.The purity that obtains the kojic acid finished product is 98.0%, and crystal is even.
Embodiment 6 repeats embodiment 1 by described same steps as, but the inoculation culture leavening temperature is 32 ℃, and acid production rate is 6.5%, and the purity that obtains the kojic acid finished product is 98.0%: crystal is even.
Embodiment 7 repeats embodiment 1 by described same steps as, but the sterilisation temp of liquid glucose is 110 ℃, and disinfecting time is 40 minutes, and acid production rate is 6.7%, and the purity that obtains the kojic acid finished product is 98.0%, and crystal is even.
Embodiment 8 repeats embodiment by described same steps as and pricks, but the lysate concentration when purifying is 9Be ', and the consumption of active carbon for decolorization is 0.1%, and acid production rate is 6.7%, and the purity that obtains the kojic acid finished product is 98.7%, and crystal is even.
Embodiment 9 repeats embodiment 1 by described same steps as, but the α-Dian Fenmei that drops into is 45 kilograms, and the inoculation fermentation temperature is 32 ℃, and acid production rate is 6.7%, and the purity that obtains the kojic acid finished product is 98.0%, and crystal is even.
Embodiment 10 repeats embodiment 1 by described same steps as, but the α-Dian Fenmei that drops into is 45 kilograms, and starch liquefacation and saccharification temperature are 70 ℃, and acid production rate is 6.7%, and the purity that obtains the kojic acid finished product is 98.0%, and crystal is even.
Embodiment 11 repeats embodiment 1 by described same steps as, but starch liquefacation and saccharification temperature are 70 ℃, and the liquid glucose sterilisation temp is 110 ℃, and disinfecting time 40 minutes: acid production rate is 6.5%, and the purity that obtains the kojic acid finished product is 98.0%, and crystal is even.
Embodiment 12 repeats embodiment 1 by described same steps as, but the α-Dian Fenmei that drops into is 45 kilograms, and the liquefaction of starch and saccharification temperature are 70 ℃, and the liquid glucose sterilisation temp is 110 ℃, is incubated 40 minutes.Acid production rate is 6.5%, and the purity that obtains the kojic acid finished product is 98.0%, and crystal is even.
Embodiment 13 repeats embodiment 1 by described same steps as, but the inoculation fermentation temperature is 36 ℃, and acid production rate is 6.5%, and the purity that obtains the kojic acid finished product is 98.1%: crystal is even.
Embodiment 14 repeats embodiment 1 by described same steps as, but starch liquefacation and saccharification temperature are 70 ℃, and the lysate concentration during purification is 9Be ', and the consumption of active carbon for decolorization is 0.2%, acid production rate is 6.7%, and the purity that obtains the kojic acid finished product is 98.0%: crystal is even.
Embodiment 15 repeats embodiment 1 by described same steps as, but the α-Dian Fenmei that drops into is 45 kilograms, starch liquefacation and saccharification temperature are 70 ℃, lysate concentration during purification is 9Be ', the consumption of active carbon for decolorization is 0.1%, acid production rate is 6.5%, and the purity that obtains the kojic acid finished product is 98.0%: crystal is even.
Claims (2)
1, a kind of kojic acid producing process, this technology is made up of mashing, inoculation fermentation and refining three steps, it is characterized in that:
Starch directly drops into fermentor tank, and the amylase of adding is α-Dian Fenmei, and add-on is 5～15u/g starch; Be warming up to 70～100 ℃ of liquefaction and saccharification;
Liquid glucose is through 110～130 ℃ of temperature, and the time is inoculated bacterial classification aspergillus oryzae Aspergillus oryzae after being sterilization in 30-40 minute, and the temperature of inoculation fermentation is 32～36 ℃, carries out ventilating fermentation, cultivates kojic acid;
The repeatedly condensing crystal product of kojic acid clear liquid is through dissolving, decolouring, when dissolving, decolouring repeatedly the concentration of the lysate of condensing crystal product be 9-12Be ', decrease temperature crystalline is purified and is obtained the kojic acid crystal of finished product.
2, kojic acid producing process according to claim 1, the discoloring agent that adopts when it is characterized in that decolouring is the gac of 0.1-5% weight.
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| Application Number | Priority Date | Filing Date | Title |
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| CN 01135102 CN1230552C (en) | 2001-11-08 | 2001-11-08 | Kojic acid producing process |
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| CN 01135102 CN1230552C (en) | 2001-11-08 | 2001-11-08 | Kojic acid producing process |
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| CN1417341A CN1417341A (en) | 2003-05-14 |
| CN1230552C true CN1230552C (en) | 2005-12-07 |
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| CN 01135102 Expired - Fee Related CN1230552C (en) | 2001-11-08 | 2001-11-08 | Kojic acid producing process |
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102321700A (en) * | 2011-08-03 | 2012-01-18 | 无锡赛德生物工程有限公司 | Kojic acid fermentation technique and apparatus |
| CN105837543B (en) * | 2016-04-01 | 2018-11-09 | 江南大学 | A method of the Hydrolysis kinetics kojic acid from zymotic fluid |
| TWI735652B (en) * | 2016-09-29 | 2021-08-11 | 心悅生醫股份有限公司 | Co-crystal and/or eutectic crystal of kojic acid, compositions comprising the same, and uses thereof |
| CN107099451A (en) * | 2017-06-12 | 2017-08-29 | 成都金开生物工程有限公司 | A kind of kojic acid production system of energy-conserving and environment-protective |
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