CN108641969A - A kind of griseofulvin production bacterium culture medium - Google Patents
A kind of griseofulvin production bacterium culture medium Download PDFInfo
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- CN108641969A CN108641969A CN201810641746.9A CN201810641746A CN108641969A CN 108641969 A CN108641969 A CN 108641969A CN 201810641746 A CN201810641746 A CN 201810641746A CN 108641969 A CN108641969 A CN 108641969A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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Abstract
The invention discloses a kind of griseofulvin to produce bacterium culture medium, it is formulated by the raw material of following weight parts ratio：700~900 parts of water, 35~70 parts of corn flour, 3~4 parts of potassium dihydrogen phosphate, 1~2 part of sodium chloride, 1~1.5 part of potassium chloride, 1~1.5 part of ammonium sulfate, 1~2 part of sodium nitrate, 1~2 part of ferrous sulfate, 3~5 parts of calcium carbonate.With it, production griseofulvin production strain quality is stable, fertility is strong, of low cost, remarkable in economical benefits.
Description
Technical field
The invention belongs to bacterium culture medium technical field, especially a kind of griseofulvin produces bacterium culture medium.
Background technology
Griseofulvin (griseofulvin) is aromatic derivant class antibiotic, it is a kind of antifungal antibiotic,
Clinically it is widely used in infection caused by treating skin shallow-layer filamentous fungi, it is significant in efficacy.Agriculturally also it is being used for anti-plant
Object nosomycosis.
Strain used in country's producing griseofulvin by fermentation is the wild type penicillium patulum that China is voluntarily screened at present
4541 bacterial strains of Penicillium patulum, the offspring N15-3-40 of the D-756 strains come through induced mutations.From fermentation side
Since method produces griseofulvin, primary carbon source raw material of the long-grained nonglutinous rice as bacterium culture medium has been used always.Long-grained nonglutinous rice raw material fecund is in south
Side, cost are higher.
Invention content
The purpose of the present invention is providing a kind of griseofulvin production bacterium culture medium, and producing griseofulvin with it produces strain
Stable quality, production capacity is strong, of low cost, remarkable in economical benefits.
The technical proposal of the invention is realized in this way：A kind of griseofulvin production bacterium culture medium, it is by following weight
The raw material of part ratio is formulated：700~900 parts of water, 35~70 parts of corn flour, 3~4 parts of potassium dihydrogen phosphate, sodium chloride 1~2
Part, 1~1.5 part of potassium chloride, 1~1.5 part of ammonium sulfate, 1~2 part of sodium nitrate, 1~2 part of ferrous sulfate, 3~5 parts of calcium carbonate.
The technical solution advanced optimized, the griseofulvin produce bacterium culture medium, it is by following weight parts ratio
Raw material be formulated：850 parts of water, 52 parts of corn flour, 3.5 parts of potassium dihydrogen phosphate, 1.5 parts of sodium chloride, 1.2 parts of potassium chloride, sulphur
1.2 parts of sour ammonium, 1.5 parts of sodium nitrate, 1.5 parts of ferrous sulfate, 4 parts of calcium carbonate.
The present invention compared to the prior art the advantages of be：
1) present invention is done process metabolism research, is passed through inspection using the carbon source long rice flour substituted with corn flour in seed formula
Surveying thalline solid content can the convenient and efficient increase ratio for compareing bacterium amount；Micro- sem observation can be with the growth step of intuitive judgment bacterium
Section.Seed solid content corn flour test group is higher than control group by 41.63% within 46 hours, 54 hours seed solid content corn flour test groups
It is higher than control group by 20.25%.The speed of growth of bacterial strain can be significantly improved.
2) culture medium of the invention can shorten 8 hours seed culture periods or more, save power.
3) present invention uses corn flour to replace primary carbon source raw material of the long rice flour as culture medium, can reduce cost of material
20~30%.
Specific implementation mode
To keep technical scheme of the present invention clearer, embodiment of the present invention is made into one below by specific embodiment
Step ground detailed description.
Embodiment 1：A kind of griseofulvin production bacterium culture medium, it is formulated by the raw material of following weight parts ratio：
850 parts of water, 52 parts of corn flour, 3.5 parts of potassium dihydrogen phosphate, 1.5 parts of sodium chloride, 1.2 parts of potassium chloride, 1.2 parts of ammonium sulfate, sodium nitrate
1.5 parts, 1.5 parts of ferrous sulfate, 4 parts of calcium carbonate.
Embodiment 2：A kind of griseofulvin production bacterium culture medium, it is formulated by the raw material of following weight parts ratio：
720 parts of water, 35 parts of corn flour, 3 parts of potassium dihydrogen phosphate, 1 part of sodium chloride, 1.5 parts of potassium chloride, 1.5 parts of ammonium sulfate, 2 parts of sodium nitrate,
2 parts of ferrous sulfate, 3 parts of calcium carbonate.
Contrast test：
Testing program：It is formulated as follows the test recipe and control formula of ratio；
Test recipe：A kind of griseofulvin production bacterium culture medium, it is formulated by the raw material of following weight parts ratio：
900 parts of water, 70 parts of corn flour, 4 parts of potassium dihydrogen phosphate, 2 parts of sodium chloride, 1 part of potassium chloride, 1 part of ammonium sulfate, 1 part of sodium nitrate, sulfuric acid
Ferrous 1 part, 4 parts of calcium carbonate.
Control formula：A kind of griseofulvin production bacterium culture medium, it is formulated by the raw material of following weight parts ratio：
900 parts of water, 70 parts of long rice flour, 4 parts of calcium carbonate, 4 parts of potassium dihydrogen phosphate, 2 parts of sodium chloride, 1 part of potassium chloride, 1 part of ammonium sulfate, nitric acid
1 part of sodium, 1 part of ferrous sulfate.
The rice spore of strain N15-3-40 is aseptically respectively connected to the seed equipped with above two culture medium
In shaking flask, cultivated under conditions of temperature is 30 DEG C, humidity is 30~50%, rotating speed is 180rpm；Six batches are done to having a competition
It tests, detects within 46 and 54 hours the content of solid content in culture solution in culture respectively, contrast test testing result is as shown in table 1.
1 test recipe of table is compared with control formula solid content
Conclusion (of pressure testing)：
From 1 data of table：The average content of solid content is in culture solution in 46 hours test recipe culture mediums of culture
22.42%, the content than solid content in culture solution in control formula culture medium is high by 6.59%；Cultivate test recipe culture in 54 hours
The content of solid content is average 23.75% in culture solution in base, than the content of solid content in culture solution in control formula culture medium
It is high by 4%；
Analysis the above results can illustrate：Replace long rice flour main as griseofulvin production bacterium culture medium using corn flour
Carbon source raw material, griseofulvin production culture propagation is fast, high-quality, and yield is high.
Claims (2)
1. a kind of griseofulvin produces bacterium culture medium, it is formulated by the raw material of following weight parts ratio：Water 700~900
Part, 35~70 parts of corn flour, 3~4 parts of potassium dihydrogen phosphate, 1~2 part of sodium chloride, 1~1.5 part of potassium chloride, ammonium sulfate 1~1.5
Part, 1~2 part of sodium nitrate, 1~2 part of ferrous sulfate, 3~5 parts of calcium carbonate.
2. griseofulvin according to claim 1 produces bacterium culture medium, it is characterised in that：It is by following weight parts ratio
Raw material be formulated：850 parts of water, 52 parts of corn flour, 3.5 parts of potassium dihydrogen phosphate, 1.5 parts of sodium chloride, 1.2 parts of potassium chloride, sulphur
1.2 parts of sour ammonium, 1.5 parts of sodium nitrate, 1.5 parts of ferrous sulfate, 4 parts of calcium carbonate.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810641746.9A CN108641969A (en) | 2018-06-21 | 2018-06-21 | A kind of griseofulvin production bacterium culture medium |
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| CN201810641746.9A CN108641969A (en) | 2018-06-21 | 2018-06-21 | A kind of griseofulvin production bacterium culture medium |
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| CN108641969A true CN108641969A (en) | 2018-10-12 |
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| CN201810641746.9A Pending CN108641969A (en) | 2018-06-21 | 2018-06-21 | A kind of griseofulvin production bacterium culture medium |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110317841A (en) * | 2019-07-10 | 2019-10-11 | 内蒙古格林特制药有限责任公司 | A kind of griseofulvin fermentation production medium |
| CN112094874A (en) * | 2020-09-16 | 2020-12-18 | 内蒙古格林特制药有限责任公司 | Culture medium for producing griseofulvin through fermentation |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006081536A (en) * | 2004-08-20 | 2006-03-30 | Denka Seiken Co Ltd | Medium for lactobacillus |
| CN101812489A (en) * | 2010-04-29 | 2010-08-25 | 赤峰制药股份有限公司 | Culture medium for producing griseofulvin by fermentation |
-
2018
- 2018-06-21 CN CN201810641746.9A patent/CN108641969A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006081536A (en) * | 2004-08-20 | 2006-03-30 | Denka Seiken Co Ltd | Medium for lactobacillus |
| CN101812489A (en) * | 2010-04-29 | 2010-08-25 | 赤峰制药股份有限公司 | Culture medium for producing griseofulvin by fermentation |
Non-Patent Citations (1)
| Title |
|---|
| 俞文和: "《新编抗生素工艺学》", 30 September 1996, 中国建材工业出版社 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110317841A (en) * | 2019-07-10 | 2019-10-11 | 内蒙古格林特制药有限责任公司 | A kind of griseofulvin fermentation production medium |
| CN110317841B (en) * | 2019-07-10 | 2022-11-22 | 内蒙古格林特制药有限责任公司 | Fermentation production culture medium for griseofulvin |
| CN112094874A (en) * | 2020-09-16 | 2020-12-18 | 内蒙古格林特制药有限责任公司 | Culture medium for producing griseofulvin through fermentation |
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