The method of hybridization of south thelenota ananas and north stichopus japonicus
Technical field
The present invention relates to aquaculture field, a kind of specifically southern plum blossom sea cucumber and the hybridization of northern apostichopus japonicus selenka, the method for breeding the new varieties sea cucumber.
Background technology
At present, the sea cucumber of China is divided into the plum blossom ginseng in southern marine site and the stichopus japonicus two big kinds in northern marine site from the place of production, plum blossom ginseng build is big, output is high, but meat is poor, the content of various elements and nutritive value are lower than stichopus japonicus, stichopus japonicus is of high nutritive value, resistance against diseases is strong, but small, yield poorly, they are in breed is bred, all adopt the high sea ginseng of same breed and female sea cucumber mating, from the conventional method of numerous self-fertile, promptly, the plum blossom ginseng is only bred the plum blossom ginseng, and stichopus japonicus only breeds stichopus japonicus, can not produce the new varieties sea cucumber that has plum blossom ginseng, two kinds of sea cucumber advantages of stichopus japonicus concurrently.
Summary of the invention
The present invention is for solving the traditional mating system of present sea cucumber from numerous self-fertile, can not produce the problem of the new varieties sea cucumber that has southern marine site plum blossom ginseng and two kinds of sea cucumber advantages of northern marine site stichopus japonicus concurrently, invent the southern marine site of a kind of usefulness plum blossom ginseng and do male parent, stichopus japonicus is cooked female parent, the method for crossbreed new varieties sea cucumber to marine site, the north.
The present invention is for solving the problems of the technologies described above, and the technical scheme of being taked is, a kind of method of hybridization of south thelenota ananas and north stichopus japonicus, and its special character is to comprise following process:
1, seed selection
Gather the plum blossom ginseng from southern marine site and plant, gather the stichopus japonicus kind from northern marine site;
2, regulate water temperature
The water temperature that adjusting plum blossom ginseng is planted was stored up 40-60 days at 15 ℃-17 ℃; The water temperature that the water temperature of adjusting stichopus japonicus kind and plum blossom ginseng are planted is synchronous, and the undulating value of pH value after intensive culture a period of time, improves 0.5 ℃ of water temperature less than 0.5 every day, reaches 20 ℃ up to water temperature;
3, combo is gone into the pond
When the gonadal maturation of plum blossom ginseng and stichopus japonicus, to select public plum blossom and join and female stichopus japonicus, combo is put into the hatchery;
4, hatching
The temperature difference of control hatchery water is less than 0.5 ℃, the PH undulating value is less than 1, the variation of ammonia nitrogen is less than 0.15, in time stir water body after parent participation is laid eggs, prevent that ovum from sinking at the bottom of the pond, replenish the needed nutriments of embryonic development such as diatom, salt algae, Chaetoceros, ocean rhodotorula, spirulina, strengthen resistance against diseases, in time wash ovum, guarantee the cleaning of ovum grain, the density of the strict control of after fertilization fertilized egg is about 5/milliliter;
5, hatching back management
After the hatching, fed two defective materials, and carried out water quality monitoring, change water in one day.
Technique effect of the present invention is, adopt above-mentioned technical scheme, can realize a kind of method of utilizing south thelenota ananas and north stichopus japonicus crossbreed new varieties sea cucumber, the sea cucumber that is bred had both had the southern plum blossom ginseng advantage that build is big, output is high, had the advantage that north stichopus japonicus is of high nutritive value, resistance against diseases is strong again.
Embodiment
1, seed selection
Gather the plum blossom ginseng from the marine site, Hainan Island and plant, gather the stichopus japonicus kind from marine site, marine site, Qingdao;
2, regulate water temperature
The plum blossom ginseng of gathering from the marine site, Hainan Island kind at first will be controlled water temperature well, regulates water temperature at 15 ℃-17 ℃, stores up about 50 days; From the stichopus japonicus kind that the Qingdao Huanghai Sea is gathered, water temperature is low, and the water temperature that adjusting water temperature and plum blossom ginseng are planted is synchronous, and the undulating value of pH value after intensive culture a period of time, improves 0.5 ℃ of water temperature less than 0.5 every day, reaches 20 ℃ up to water temperature;
3, combo is gone into the pond
When the gonadal maturation of plum blossom ginseng and stichopus japonicus, to select public plum blossom and join and female stichopus japonicus, combo is put into the hatchery;
4, hatching
Parent participation is very high to water quality requirement before and after laying eggs, the temperature difference of control Chi Shui is less than 0.5 ℃, the PH undulating value is less than 1, the variation of ammonia nitrogen is less than 0.15, keep sufficient oxygen, parent participation is laid eggs and was stirred water body in back 20 minutes, prevents that ovum from sinking at the bottom of the pond, because of the death of cancerating of bottom, and additional diatom, the salt algae, Chaetoceros, ocean rhodotorula, the needed nutriment of embryonic developments such as spirulina, strengthen embryo's resistance against diseases, in time wash ovum, guarantee the cleaning of ovum grain, can in time be fertilized, the density of the strict control of after fertilization fertilized egg is suitable with 5/milliliter, hatches as the excessive pond of dividing of density;
5, hatching back management
To conscientiously strengthen management after the hatching, feed two defective materials, and carried out water quality monitoring, change water in one day.
Above process can realize a kind of new varieties sea cucumber, and this sea cucumber had both had the Hainan Island plum blossom ginseng advantage that build is big, output is high, had the advantage that north stichopus japonicus is of high nutritive value, resistance against diseases is strong again.