CN101785476A - Cryoprotective agent for protected vegetable and preparation method thereof - Google Patents
Cryoprotective agent for protected vegetable and preparation method thereof Download PDFInfo
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- CN101785476A CN101785476A CN201010110224A CN201010110224A CN101785476A CN 101785476 A CN101785476 A CN 101785476A CN 201010110224 A CN201010110224 A CN 201010110224A CN 201010110224 A CN201010110224 A CN 201010110224A CN 101785476 A CN101785476 A CN 101785476A
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Abstract
The invention discloses a cryoprotective agent for protected vegetable and a preparation method thereof. The cryoprotective agent comprises the following components according to parts by weight: 1.00-2.50 parts of glucose, 0.03-0.20 part of polyethylene glycol (PEG 400), 0.01-0.03 part of streptomycin, 0.10-0.30 part of calcium chloride and 96.97-98.86 parts of distilled water. The preparation method comprises the following steps of respectively dissolving the 1.00-2.50 parts of glucose, 0.03-0.20 part of polyethylene glycol, 0.01-0.03 part of streptomycin and 0.10-0.30 part of calcium chloride in 10 parts of the distilled water firstly and uniformly mixing, and then adding the residual distilled water, uniformly mixing and placing for 0.5 hour under 25 DEG C to obtain the preparation. The cryoprotective agent for the vegetable has evident effect, convenient use and low cost and can be widely used for production of protected vegetable of North China. The cryoprotective agent can prevent and slow the loss of the vegetable by the low temperature and guarantees the production efficiency and stable income of the vegetable grower.
Description
Technical field
The invention belongs to agricultural planting administrative skill field, particularly a kind of cryoprotective agent that is applied to facilities vegetable and preparation method thereof.
Background technology
Low temperature is one of critical limitation factor that influences the high-quality and efficient production of China North China facilities vegetable winter-spring season.In recent years, when global climate warms gradually, because extreme climate change frequency increase, uneven in temperature causes the low temperature disaster to take place frequently; Simultaneously, development along with Chinese national economy and society, the pattern of farming of facilities vegetable and kind layout constantly change (plantation adapts to the more vegetables or the kind of warm climate condition under the condition that warms), the risk of its freeze injury of catching a cold is strengthened, and also is that one of frequent day by day major reason takes place China's low temperature disaster.Often because of there's a sudden fall in temperature and continuous low temperature poor growth, the physiological activity in cloudy weather for several days running snow sky are lacked of proper care, change melon, macerated root, the serious underproduction, even total crop failure cause enormous economic loss to facilities vegetable.At present, take the measure of anti-low temperature to have in facilities vegetable production: (1) adopts kind low temperature resistant, the low light level; (2) take to be incubated in the facility, to heat; (3) adopt suitable field management measure, as measures such as many organic fertilizers, low temperature hardening and employing drip irrigation techniques; (4) adopt the foliage spray growth regulator.Above-mentioned anti-low temperature measure can be alleviated the harm of low temperature to vegetables to a certain extent, but still exist the cost of investment height, time-consumingly take a lot of work, problem such as effect instability.
Summary of the invention
Deficiency at above-mentioned prior art existence, the object of the invention provides anti-cryogenic preparation of a kind of facilities vegetable and preparation method thereof, said preparation uses before and after low temperature takes place, can prevent and slow down the loss that low temperature causes vegetables, the problem that solve the effect instability that exists in the existing anti-low temperature measure, time-consumingly take a lot of work, cost of investment is high.
For realizing above-mentioned task, the present invention adopts following technical scheme:
A kind of anti-cryogenic preparation that is applied to facilities vegetable, form by the component of following weight content:
1.00 parts-2.50 parts of glucose
0.03 part-0.20 part of polyethylene glycol (PEG400)
0.01 part-0.03 part of streptomycin
0.10 part-0.30 part in calcium chloride
96.97 parts-98.86 parts of distilled water
A kind of preparation method who is applied to the anti-cryogenic preparation of facilities vegetable: preparation process is as follows:
(1) gets 1.00 parts of-2.50 parts of glucose, 0.03 part of-0.20 part of polyethylene glycol (PEG400), 0.01 part of-0.03 part of streptomycin, 0.10 part of-0.30 part of calcium chloride;
(2) get 96.97 parts-98.86 parts distilled water;
(3) earlier with 1.00 parts of-2.50 parts of glucose, 0.03 part of-0.20 part of polyethylene glycol, 0.01 part of-0.03 part of streptomycin, 0.10 part of-0.30 part calcium chloride is dissolved in respectively in 10 parts the distilled water, several medicine mixings add remaining distilled water again, mixing, 25 ℃ are placed 0.5 hour is the cost preparation.
The glucose of one of component of the present invention when being applied to vegetable crop, can improve cell inclusion concentration, and the reduction freezing point is prevention and slow down the main protection material of low temperature adverse circumstance.
Polyethylene glycol is applied to vegetable crop as Osmolyte regulator, can improve the seedling chlorophyll content, increases the protective enzyme activity, improves crop cold resistance and drought resistance.Zhang Chunmei research is pointed out, under the drought stress of Polyethylene Glycol Simulated, abscisic acid (ABA) and the equal run-up of jasmonic (JA) content in 2 kind tomato seedling roots and the leaf, free state spermidine (fSpd), spermine (fSpm), putrescine (fPut) content and PAO (PAO) activity all rise earlier and afterwards descend.The rising of ABA and JA content is induced in the accumulation of endogenous polyamines, thereby causes the drought resistance of tomato seedling to strengthen.(Zhang Chunmei.The variation of ABA, JA and polyamine content and polyamine oxidase enzymic activity in the tomato seedling under polyethylene glycol is coerced.Plant Physiology Communications, in August, 2008,689-692 page or leaf.)
Streptomycin is having important function as one of treatment main medicine lungy aspect the anti-low temperature of vegetables, and it can kill the ice-nucleus bacterium in vegetable surface and the body, and reduces the vegetables freezing point, plays the effect that improves vegetables cold resistance.
Ca
2+Be second envoy of conduction plant cold-information.Ca
2+Having two aspect effects aspect the freeze proof power of raising plant: the one, direct effect; promptly by stabilized cell wall, membrane structure and raising protective enzyme activity anti-cold power (Boss WF with the enhancing plant; Mott RL.Effects of divalentcations and Polyethylene glycol on the membrane Fluidity ofProtoPlasts.Plant Physiol; 1980; 66:835-837) two is indirectly-actings; as the second messenger; promptly by transmitting cold-information, the expression of inducing anti-freeze gene is to improve the freeze proof power (Jiao Yansheng of plant.Calcium is coerced down the influence of cucumber seedling active oxygen and polyamines metabolism to the rhizosphere hypoxemia.Agricultural University Of Nanjing, thesis for the doctorate.2007)
The using method of vegetable cryoprotective agent of the present invention is: foliage-spray is 1-2 time before and after low temperature arrives, and consumption is as the criterion to squirt the blade face.
Range of application: facilities vegetable such as tomato, cucumber, custard squash, capsicum etc.
Result of the test shows, sprays before and after low temperature season arrives, and can significantly improve the activity of vegetables POD enzyme, reduces the activity of SOD enzyme, effectively reduces the index that damages to plants caused by sudden drop in temperature of facilities vegetable, improves output, and anti-cryogenic effect is better.Its antifreeze mechanism is: when temperature is reduced to critical low temperature, can kill the ice-nucleus bacterium of vegetable surface and improve cell sap concentration, reduce freezing point; And can form layer protecting film at vegetable surface, and make crop close pore naturally, effectively suppress distributing of plant nature heat and moisture, thus damaging to plants caused by sudden drop in temperature of preventing that low temperature from causing; Contained physiological activator can be regulated the physiological activity of vegetables, thereby reaches antifreeze freeze proof, promotes growth, protects flower, fruit retention, improves multi-efficiencies such as percentage of fertile fruit.
Damage to plants caused by sudden drop in temperature index and be meant the injured leaf area size of different vegetable under low temperature stress is divided into 1,2,3,4 different stage, 1 grade is the lightest, and 4 grades are the heaviest, calculate with following formula:
Damage to plants caused by sudden drop in temperature index=(1 * 1 grade blade number+2 * 2 grade blade numbers+3 * 3 grade blade numbers+4 * 4 grade blade numbers)/(total strain number * every strain number of blade * 4) * 100
Index * 100 are damaged to plants caused by sudden drop in temperature in anti-cryogenic effect %=(contrast is damaged to plants caused by sudden drop in temperature index-processing and damaged to plants caused by sudden drop in temperature index)/contrast
Vegetable cryoprotective agent effect of the present invention is remarkable, easy to use, and raw material components is that cryoprotective agent is the vegetable nutrient material again, and is nontoxic to vegetables, harmless, is widely used in the production of northern China facilities vegetable.The present invention can prevent and slow down the loss that low temperature causes vegetables, ensures vegetable grower's productivity effect and stable income.
Embodiment
Embodiment 1
Get 1.00 gram glucose, 0.1 gram polyethylene glycol, 0.01 gram streptomycin, 0.20 gram calcium chloride, 98.69 gram distilled water earlier, 1.00 gram glucose, 0.1 gram polyethylene glycol, 0.01 gram streptomycin, 0.20 gram calcium chloride, 98.69 gram distilled water are dissolved in respectively in the distilled water of 10 grams, several medicines mix, again remaining distilled water is added, left standstill under the mixing, normal temperature 0.5 hour.Preparation of the present invention is applied to blue or green generation custard squash early, has obtained good result.Water seed soaking vernalization on June 12 in 2009,9cm * 9cm feeding block seedlings raising, one heart stage of three leaves, spray preparation of the present invention, contrast is handled with clear water, second day immigration climatic cabinate carries out low temperature stress to be handled, temperature is 6 ℃, temperature photoperiod 10h (daytime)/14h (night), handle measure seedling leaves behind the 3d damage to plants caused by sudden drop in temperature the exponential sum physical signs.The material of participating in the experiment in every processing is 8 strains, repeats 3 times, adopts the DPS statistical method that data are carried out statistical analysis, and result of the test shows that it is 23.23 that index is damaged to plants caused by sudden drop in temperature in processing, and contrast is 32.63, and preventive effect is 28.81%; Handling the POD enzymic activity is 348.24U/gmin, contrasts to be 302.85U/gmin, is 114.99% of contrast; Treatment S OD enzymic activity is 468.80U/gmin, contrasts to be 582.03U/gmin, is 80.55% of contrast.
Embodiment 2
Get 1.00 gram glucose, 0.1 gram polyethylene glycol, 0.02 gram streptomycin, 0.20 gram calcium chloride, 98.68 gram distilled water earlier, with 1.00 gram glucose, 0.1 gram polyethylene glycol, 0.02 gram streptomycin, 0.20 gram calcium chloride, 98.68 gram distilled water, be dissolved in respectively in the distilled water of 10 grams, several medicines mix, again remaining distilled water is added, left standstill under the mixing, normal temperature 0.5 hour.Early blue or green generation custard squash test: sowing on August 7th, 2009, establish 1 processing, 1 contrast, three repetitions.The material of participating in the experiment in every processing is 8 strains.Sub-district area 3m
2Sprayed preparation of the present invention on October 10, contrast is handled with clear water; Sprayed said preparation for the second time on October 17.Record output.Result of the test shows that processing output is 2.6kg, contrasts to be 1.5kg, and comparison is according to volume increase 73.33%.
Embodiment 3
Get 1.50 gram glucose, 0.15 gram polyethylene glycol, 0.02 gram streptomycin, 0.25 gram calcium chloride, 98.08 gram distilled water earlier, 1.50 gram glucose, 0.15 gram polyethylene glycol, 0.02 gram streptomycin, 0.25 gram calcium chloride, 98.08 gram distilled water are dissolved in respectively in the distilled water of 10 grams, several medicines mix, again remaining distilled water is added, left standstill under the mixing, normal temperature 0.5 hour.The anti-king's custard squash test of heat: water seed soaking vernalization on July 4 in 2009,9cm * 9cm feeding block seedlings raising, one heart stage of three leaves, spray said preparation, contrast is handled with clear water, second day immigration climatic cabinate carries out low temperature stress to be handled, temperature is 6 ℃, and temperature photoperiod 10h (daytime)/14h (night) handles the index that damages to plants caused by sudden drop in temperature of measuring seedling leaves behind the 3d.The material of participating in the experiment in every processing is 8 strains, repeats 3 times, adopts the DPS statistical method that data are carried out statistical analysis, and result of the test shows that it is 35.86 that index is damaged to plants caused by sudden drop in temperature in processing, and contrast is 52.39, and preventive effect is 31.55%; Handling the POD enzymic activity is 438.65U/gmin, contrasts to be 322.58U/gmin, is 135.98% of contrast; Treatment S OD enzymic activity is 486.80U/gmin, contrasts to be 652.23U/gmin, is 74.64% of contrast.
Embodiment 4
Get 2.50 gram glucose, 0.15 gram polyethylene glycol, 0.03 gram streptomycin, 0.20 gram calcium chloride, 97.12 gram distilled water earlier, 2.50 gram glucose, 0.15 gram polyethylene glycol, 0.03 gram streptomycin, 0.20 gram calcium chloride, 97.12 gram distilled water are dissolved in respectively in the distilled water of 10 grams, several medicines mix, again remaining distilled water is added, left standstill under the mixing, normal temperature 0.5 hour.In Shanxi tomato No. 4 test: on June 8th, 2009 point disk seedling growing, 1 heart stage of 4 leaves, spray preparation of the present invention, contrast is handled with clear water, second day immigration climatic cabinate carries out low temperature stress to be handled, temperature is 5 ℃, temperature photoperiod 10h (daytime)/14h (night), handle measure seedling leaves behind the 10d damage to plants caused by sudden drop in temperature the exponential sum physical signs.The material of participating in the experiment in every processing is 12 strains, repeats 3 times, adopts the DPS statistical method that data are carried out statistical analysis.Result of the test shows that it is 28.20 that index is damaged to plants caused by sudden drop in temperature in processing, and contrast is 39.42, and preventive effect is 28.46%; Handling the POD enzymic activity is 589.03U/gmin, contrasts to be 395.38U/gmin, is 148.98% of contrast; Treatment S OD enzymic activity is 436.40U/gmin, contrasts to be 585.43U/gmin, is 74.54% of contrast.
Embodiment 5
Get 2.50 gram glucose, 0.05 gram polyethylene glycol, 0.03 gram streptomycin, 0.10 gram calcium chloride, 97.32 gram distilled water earlier, in 2.50 gram glucose, 0.05 gram polyethylene glycol, 0.03 gram streptomycin, 0.10 gram calcium chloride, 97.32 gram distilled water, several medicines mix, again remaining distilled water is added, left standstill under the mixing, normal temperature 0.5 hour.No. 1 capsicum test of Shanxi point green pepper: on August 5th, 2009 point disk seedling growing, 1 heart stage of 4 leaves, spray preparation of the present invention, with the clear water is contrast, second day immigration climatic cabinate carries out low temperature stress to be handled, temperature is 6 ℃, temperature photoperiod 10h (daytime)/14h (night), that handles 10d " Invest, Then Investigate " seedling leaves damages to plants caused by sudden drop in temperature the exponential sum physical signs.The material of participating in the experiment in every processing is 8 strains, repeats 3 times, adopts the DPS statistical method that data are carried out statistical analysis.Result of the test shows that it is 46.39 that index is damaged to plants caused by sudden drop in temperature in processing, and contrast is 68.38, and preventive effect is 32.16%; Handling the POD enzymic activity is 555.33U/gmin, contrasts to be 383.64U/gmin, is 144.75% of contrast; Treatment S OD enzymic activity is 464.21U/gmin, contrasts to be 687.51U/gmin, is 67.52% of contrast.
Embodiment 6
Get 1.50 gram glucose, 0.20 gram polyethylene glycol, 0.02 gram streptomycin, 0.10 gram calcium chloride, 98.18 gram distilled water earlier, 1.50 gram glucose, 0.20 gram polyethylene glycol, 0.02 gram streptomycin, 0.10 gram calcium chloride, 98.18 gram distilled water are dissolved in respectively in the distilled water of 10 grams, several medicines mix, again remaining distilled water is added, left standstill under the mixing, normal temperature 0.5 hour.Excellent No. 31 cucumber tests in Tianjin: on September 7th, 2009 presoaking and germinating, 8cm * 8cm feeding block seedlings raising, one heart stage of two leaves, spray said preparation, contrast spray equivalent clear water.Second day immigration climatic cabinate low temperature treatment, temperature is 6 ℃, temperature photoperiod 10h (daytime)/14h (night), that handles 5d " Invest, Then Investigate " seedling leaves damages to plants caused by sudden drop in temperature the exponential sum physical signs.Result of the test shows that it is 32.69 that index is damaged to plants caused by sudden drop in temperature in processing, and contrast is 50.38, and preventive effect is 35.11%; Handling the POD enzymic activity is 489.62U/gmin, contrasts to be 357.75U/gmin, is 136.86% of contrast; Treatment S OD enzymic activity is 382.66U/gmin, contrasts to be 507.78U/gmin, is 75.36% of contrast.
Embodiment 7
Get 1.00 gram glucose, 0.10 gram polyethylene glycol, 0.02 gram streptomycin, 0.20 gram calcium chloride, 98.68 gram distilled water earlier, 1.00 gram glucose, 0.10 gram polyethylene glycol, 0.02 gram streptomycin, 0.20 gram calcium chloride, 98.68 gram distilled water are dissolved in respectively in the distilled water of 10 grams, several medicines mix, again remaining distilled water is added, leaving standstill 0.5 hour under the mixing, normal temperature is the cost preparation.Blue or green generation custard squash early, on August 7th, 2009 grew seedlings, and established 1 processing, and 1 contrast repeats for three times.The material of participating in the experiment in every processing is 8 strains.One heart stage of three leaves, spray preparation of the present invention, and contrast is handled with clear water, second day immigration climatic cabinate low temperature treatment, and temperature is 6 ℃, temperature photoperiod 10h (daytime)/14h (night) handles the index that damages to plants caused by sudden drop in temperature of 5d " Invest, Then Investigate " seedling leaves; Field trial is carried out in field planting subsequently, 1 processing, 1 contrast, three repetitions.The material of participating in the experiment in every processing is 8 strains, sub-district area 3m
2Sprayed October 10 and sprayed said preparation for the second time on October 17.Record output.Result of the test shows that it is 30.97 that index is damaged to plants caused by sudden drop in temperature in processing, and contrast is 43.25, and preventive effect is 28.39%; Processing output is 6.8kg, contrasts to be 4.4kg, and comparison is according to volume increase 54.54%.
Claims (2)
1. cryoprotective agent that is applied to facilities vegetable, it is characterized in that: the component by following weight content is formed:
1.00 parts-2.50 parts of glucose
0.03 part-0.20 part of polyethylene glycol (PEG400)
0.01 part-0.03 part of streptomycin
0.10 part-0.30 part in calcium chloride
96.97 parts-98.86 parts of distilled water
2. according to the described a kind of preparation method who is applied to the cryoprotective agent of facilities vegetable of claim 1, it is characterized in that: preparation process is as follows:
(1) gets 1.00 parts of-2.50 parts of glucose, 0.03 part of-0.20 part of polyethylene glycol (PEG400), 0.01 part of-0.03 part of streptomycin, 0.10 part of-0.30 part of calcium chloride;
(2) get 96.97 parts-98.86 parts distilled water;
(3) earlier with 1 part of-2.5 parts of glucose, 0.03 part of-0.2 part of polyethylene glycol, 0.01 part of-0.03 part of streptomycin, 0.10 part of-0.30 part calcium chloride is dissolved in respectively in 10 parts the distilled water, several medicine mixings add remaining distilled water again, mixing, 25 ℃ are placed 0.5 hour is the cost preparation.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010110224XA CN101785476B (en) | 2010-02-08 | 2010-02-08 | Cryoprotective agent for protected vegetable and preparation method thereof |
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| CN201010110224XA CN101785476B (en) | 2010-02-08 | 2010-02-08 | Cryoprotective agent for protected vegetable and preparation method thereof |
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| CN101785476A true CN101785476A (en) | 2010-07-28 |
| CN101785476B CN101785476B (en) | 2012-08-01 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111011394A (en) * | 2019-12-12 | 2020-04-17 | 山东省农作物种质资源中心 | Wheat cold-resistant inducer and application method thereof |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101589713B (en) * | 2009-06-24 | 2012-05-09 | 山西省农业科学院蔬菜研究所 | Cryoprotectant applied to facility vegetables and preparation method thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111011394A (en) * | 2019-12-12 | 2020-04-17 | 山东省农作物种质资源中心 | Wheat cold-resistant inducer and application method thereof |
| CN111011394B (en) * | 2019-12-12 | 2021-02-02 | 山东省农作物种质资源中心 | Wheat cold-resistant inducer and application method thereof |
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