CN101779670B - Low-temperature-resistance growth substance applied to corns and preparation method thereof - Google Patents
Low-temperature-resistance growth substance applied to corns and preparation method thereof Download PDFInfo
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- CN101779670B CN101779670B CN201010110221A CN201010110221A CN101779670B CN 101779670 B CN101779670 B CN 101779670B CN 201010110221 A CN201010110221 A CN 201010110221A CN 201010110221 A CN201010110221 A CN 201010110221A CN 101779670 B CN101779670 B CN 101779670B
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Abstract
The invention discloses a low-temperature-resistance growth substance applied to corns and a preparation method thereof. The preparation is prepared from the following ingredients in percentage by weight: 0.05 part to 2.00 parts of glucose, 0.05 part to 0.15 part of polyethyleneglycol (PEG400), 1.50 parts to 3.00 parts of choline chloride, 0.20 part to 0.40 part of calcium chloride and 94.45 parts to 97.75 parts of distilled water. The preparation method comprises the following steps: firstly, respectively dissolving 0.05 part to 2.00 parts of glucose, 0.05 part to 0.15 part of polyethyleneglycol (PEG400), 1.50 parts to 3.00 parts of choline chloride, and 0.20 part to 0.40 part of calcium chloride into 10 parts of distilled water; uniformly mixing several kinds of medicine; then, adding the rest distilled water; uniformly mixing the materials; and obtaining the preparation after still standing for 0.5 hour at the normal temperature. The low-temperature-resistance growth substance of the invention has the advantages of obvious low-temperature-resistance effect, convenient use and low cost. When being used during the coming of the low temperature in the corn seedling period, the low-temperature-resistance growth substance can obtain good low-temperature-resistance effect, and the loss caused by temporary temperature reduction can be greatly reduced.
Description
Technical field
The invention belongs to agricultural planting administrative skill field, particularly a kind of low-temperature-resistance growth substance that is applied to corn and preparation method thereof.
Background technology
Corn is one of main cereal crops of China, and having a strong impact on the growing of corn, even causing the significantly underproduction because the influence of late frost and cold in the late spring often suffers low temperature seedling stage.In recent years, because extreme climate change frequency increase, uneven in temperature, the low temperature disaster takes place frequently, and corn is also increased by the frequency of low temperature disaster thereupon, becomes one of critical limitation factor of corn high-efficiency high-quality production.The measure of the anti-low temperature of in Maize Production, taking at present, has: low temperature resistant new varieties are adopted in (1); (2) take cultivation step, improve ground temperature like measures such as plastic mulching, increasing application phosphorus potassium fertilizer, seedling raising and transplanting; (3) adopt the foliage-spray growth regulator, the physiological activity like adjusting corns such as paclobutrazol, ancymidols reaches low temperature resistant effect.Above-mentioned anti-low temperature measure can be alleviated the influence of low temperature to corn to a certain extent, but still exist time-consumingly take a lot of work, problem such as effect instability, also lack efficiently and easily corn technology for resisting low temperature through product.
Summary of the invention
Deficiency to above-mentioned prior art existence; The object of the invention provides a kind of corn low-temperature-resistance growth substance and preparation method thereof; Said preparation sprays before and after low temperature takes place; Can prevent and slow down the loss that low temperature causes corn, solve the problem that the effect that exists in the existing anti-low temperature measure unstablely, time-consuming is taken a lot of work, cost of investment is high.
For realizing above-mentioned task, the present invention adopts following technical solution:
A kind of low-temperature-resistance growth substance that is applied to corn, form by following components in weight percentage:
A kind of preparation method who is applied to the low-temperature-resistance growth substance of corn: preparation process is following:
(1) gets 0.05 part of-2.00 parts of glucose, 0.05 part of-0.15 part of polyethylene glycol, 1.50 parts of-3.00 parts of Choline Chlorides, 0.20 part of-0.40 part of calcium chloride;
(2) get 94.45 parts-97.75 parts distilled water;
(3) earlier with 0.05 part of-2.00 parts of glucose, 0.05 part of-0.15 part of polyethylene glycol, 1.50 parts of-3.00 parts of Choline Chlorides; 0.20 part of-0.40 part calcium chloride is dissolved in respectively in 10 parts the distilled water, several kinds of medicine mixings add remaining distilled water again; Mixing, 25 ℃ are placed 0.5 hour is the cost preparation.
The glucose of one of component of the present invention can improve crop cell inclusion concentration, reduces freezing point, prevents the crop frostbite, is prevention and slows down one of main protection material of low temperature adverse circumstance, at present existing application the aspect the anti-low temperature of plant.
Polyethylene glycol is a kind of high molecular polymer, and as Osmolyte regulator, water imbibition is extremely strong, and penetration is strong, can form diaphragm at plant surface, prevents moisture loss, plays the cold resistant effect of enhancement of plant.
Ca
2+, when plant experience chilling injury is coerced, have the effect of conduction born of the same parents external information to born of the same parents' internal information, and then cause a series of biochemical reactions as the second messenger, it is movable to regulate plant physiology, improves the interior environment of born of the same parents, the effect that improves cold resistance of plant property.Ca in addition
2+Can also increase stomatal conductance, improve CO through the protection plasma structure
2Supply improves the active and carboxylation efficient of blade RuBP-Case simultaneously, and (Su Changhong, clock is beautiful, kingly way light, Li Maosong for the photosynthetic capacity of promotion leaf plant mesophyll cell.Several kinds of chemical substances are to tomato seedling tolerance to low temperature and weak light Research on ability.The natural calamity journal, 2006,15 (6): 312-316).
The Choline Chloride of one of composition of the present invention can be regulated plant stomata and open and close, and under low temperature stress, can make plant close pore, prevents scattering and disappearing of plant intimate heat and moisture, improves the plant frigostabile performance; Can improve anti-oxidant type of enzymic activity of plant, remove harmful free radical in the body, the cell membrane lipid of seedling such as protection paddy rice and corn is not oxidized or alleviate it by the peroxidating degree, is bringing into play important effect at aspects such as improving the plant frigostabile ability.(Liang Yuzhou, He Ruotian.Choline Chloride is to the protective effect of the rice seedling under the low temperature stress.The rice in China science, 1999,13 (1): 31-35.He Bing, Xu Hongyuan, He Ruotian.Choline Chloride is to the research of corn seedling blade membrane structure defense mechanism under the drought stress.Guangxi agro-ecology science, 1999,18 (4): 253-257.)
The method for using of corn low-temperature-resistance growth substance of the present invention is: foliage-spray is 1-2 time before and after low temperature arrives, and consumption is as the criterion to squirt the blade face.
Range of application: corn.
Result of the test shows, before and after low temperature season arrives, sprays, and can significantly improve maize leaf proline and soluble sugar content, helps growing of corn seedling under the cryogenic conditions, alleviates and damages to plants caused by sudden drop in temperature, and anti-cryogenic effect is better.Its antifreeze mechanism is: when temperature was reduced to critical low temperature, the physiological activity of active principle scalable seedling improved cell sap concentration, reduces freezing point, prevents to endure cold; And can form layer protecting film at vegetable surface, and make crop close pore naturally, effectively suppress distributing of plant intimate heat and moisture, thus damaging to plants caused by sudden drop in temperature of preventing that low temperature from causing.
Corn low-temperature-resistance growth substance of the present invention, anti-cryogenic effect is remarkable, and is easy to use, and raw material components is that cryoprotective agent is the vegetable nutrient material again, and nontoxic, result of use was better when met with microthermal climate the seedling stage of corn.The present invention can prevent and slow down low temperature to the loss that corn seedling causes, and reduces risks, and ensures the economic benefit of peasant planting corn.
Embodiment
Embodiment 1
Get 1.00 gram glucose, 0.15 gram polyethylene glycol, 1.50 gram Choline Chlorides, 0.40 gram calcium chloride, 96.95 gram distilled water earlier; 1.00 gram glucose, 0.15 gram polyethylene glycol, 1.50 gram Choline Chlorides, 0.40 gram calcium chloride are dissolved in respectively in the distilled water of 10 grams; Several kinds of medicines mix; Remaining distilled water is added, leaving standstill 0.5 hour under the mixing, normal temperature is the cost preparation again.Preparation of the present invention is applied to beautiful 335 corns of elder generation obtains good result.On June 12nd, 2008 changed on the culture dish that is lined with moistening filter paper behind the water seed soaking 16h, put in 25 ℃ of climatic cabinates and germinateed, and kept seed fully moistening.When treating seedling length to 2 leaves, 1 heart, spray preparation of the present invention, contrast is handled with clear water, carries out 5 ℃ of low temperature stress and handles in second day.The temperature photoperiod is 10h (daytime)/14h (night), measures the proline and the soluble sugar content of seedling leaves behind the processing 4d.The material of participating in the experiment in every processing is 12 strains, repeats 3 times, adopts the DPS statistical method that data are carried out statistical analysis.Result of the test shows that handling proline content is 0.36%, and contrast is 0.15%, is treated to 2.4 times of contrast; Processing soluble sugar content is 1.42%, and contrast is 0.62%, is treated to 2.29 times of contrast.Handling back proline and soluble sugar content significantly increases, and has obviously strengthened the anti-low temperature ability of corn.
Embodiment 2
Get 0.5 gram glucose, 0.05 gram polyethylene glycol, 2.50 gram Choline Chlorides, 0.30 gram calcium chloride, 96.60 gram distilled water earlier; 0.5 gram glucose, 0.05 gram polyethylene glycol, 2.50 gram Choline Chlorides, 0.30 gram calcium chloride are dissolved in respectively in the distilled water of 10 grams; Several kinds of medicines mix; Remaining distilled water is added, leaving standstill 0.5 hour under the mixing, normal temperature is the cost preparation again.Preparation of the present invention is applied to No. 26 corns of Da Feng.On June 20th, 2008 changed on the culture dish that is lined with moistening filter paper behind the water seed soaking 16h, put in 25 ℃ of climatic cabinates and germinateed, and kept seed fully moistening.When treating seedling length to 2 leaves, 1 heart, spray preparation of the present invention, contrast is handled with clear water, carries out 5 ℃ of low temperature stress processing in second day, and the temperature photoperiod is 10h (daytime)/14h (night), measures the proline and the soluble sugar content of seedling leaves behind the processing 4d.The material of participating in the experiment in every processing is 12 strains, repeats 3 times, adopts the DPS statistical method that data are carried out statistical analysis.Result of the test shows that handling proline content is 0.47%, and contrast is 0.17%, is treated to 2.76 times of contrast; Processing soluble sugar content is 2.04%, and contrast is 0.91%, is treated to 2.24 times of contrast.Said preparation has strengthened the anti-low temperature ability of corn seedling.
Embodiment 3
Get 2.00 gram glucose, 0.10 gram polyethylene glycol, 3.00 gram Choline Chlorides, 0.20 gram calcium chloride, 94.70 gram distilled water earlier; 2.00 gram glucose, 0.10 gram polyethylene glycol, 3.00 gram Choline Chlorides, 0.20 gram calcium chloride are dissolved in respectively in the distilled water of 10 grams; Several kinds of medicines mix; Remaining distilled water is added, leaving standstill 0.5 hour under the mixing, normal temperature is the cost preparation again.Preparation of the present invention is applied to Zheng Dan 958 and Jin Yu 811 corns, and anti-cryogenic effect is good.Be seeded in field (1 day-10 May in 2009, daily temperature was lower) on April 20th, 2009.April 28 emerged, and 1 heart stage of 1 leaf sprays calcium preparation, and contrast is handled with clear water, and every processing 30 strains repeat sub-district floor space 10.05m 3 times
2, 15 days " Invest, Then Investigate " seedling dry weight and fresh weight of plant seedlings and strong sprout index.The result is following:
Corn dry weight and fresh weight of plant seedlings behind table 1 spraying preparation
Maize leaf proline and soluble sugar content behind table 2 spraying preparation
Learn by table 1 and table 2, spray said preparation after, the proline of corn and soluble sugar content increase, dry weight and fresh weight of plant seedlings increases, and shows that this cryogenic preparation has facilitation at hypothermic phase to the growths of 2 corn variety seedling phases.
Claims (2)
1. low-temperature-resistance growth substance that is applied to corn is characterized in that: be made up of following components in weight percentage:
2. according to the described a kind of preparation method who is applied to the low-temperature-resistance growth substance of corn of claim 1, it is characterized in that: preparation process is following:
(1) gets 0.05 part of-2.00 parts of glucose, 0.05 part of-0.15 part of polyethylene glycol, 1.50 parts of-3.00 parts of Choline Chlorides, 0.20 part of-0.40 part of calcium chloride;
(2) get 94.45 parts-97.75 parts distilled water;
(3) earlier with 0.05 part of-2.00 parts of glucose; 0.05 part-0.15 part of polyethylene glycol, 1.50 parts of-3.00 parts of Choline Chlorides, 0.20 part of-0.40 part of calcium chloride are dissolved in respectively in 10 parts the distilled water; Several kinds of medicines mix; Remaining distilled water is added, leaving standstill 0.5 hour under the mixing, normal temperature is the cost preparation again.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201010110221A CN101779670B (en) | 2010-02-08 | 2010-02-08 | Low-temperature-resistance growth substance applied to corns and preparation method thereof |
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| CN201010110221A CN101779670B (en) | 2010-02-08 | 2010-02-08 | Low-temperature-resistance growth substance applied to corns and preparation method thereof |
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| CN101779670A CN101779670A (en) | 2010-07-21 |
| CN101779670B true CN101779670B (en) | 2012-10-03 |
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| CN102845424A (en) * | 2012-08-28 | 2013-01-02 | 江苏徐淮地区淮阴农业科学研究所 | Low temperature resistant regulator for sheltered cucumber and preparation method thereof |
| CN103202315A (en) * | 2013-04-07 | 2013-07-17 | 江苏徐淮地区淮阴农业科学研究所 | Low-temperature-resistant modifier of wheat and preparation method of modifier |
| CN106818865A (en) * | 2016-12-30 | 2017-06-13 | 新昌县派特普科技有限公司 | Low-temperature-resistance growth substance for corn and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101589713A (en) * | 2009-06-24 | 2009-12-02 | 山西省农业科学院蔬菜研究所 | A kind of anti-cryogenic preparation that is applied to facilities vegetable and preparation method thereof |
| CN101595873A (en) * | 2009-06-29 | 2009-12-09 | 山西省农业科学院蔬菜研究所 | A kind of facility vegetable cryoprotective agent |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101589713A (en) * | 2009-06-24 | 2009-12-02 | 山西省农业科学院蔬菜研究所 | A kind of anti-cryogenic preparation that is applied to facilities vegetable and preparation method thereof |
| CN101595873A (en) * | 2009-06-29 | 2009-12-09 | 山西省农业科学院蔬菜研究所 | A kind of facility vegetable cryoprotective agent |
Non-Patent Citations (1)
| Title |
|---|
| 康国章,等.几种外源物质提高植物抗冷力的生理机制.《植物生理学通讯》.2001,第38卷(第2期),第193-197页. * |
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