CN101589713B - Cryoprotectant applied to facility vegetables and preparation method thereof - Google Patents
Cryoprotectant applied to facility vegetables and preparation method thereof Download PDFInfo
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- CN101589713B CN101589713B CN2009100745583A CN200910074558A CN101589713B CN 101589713 B CN101589713 B CN 101589713B CN 2009100745583 A CN2009100745583 A CN 2009100745583A CN 200910074558 A CN200910074558 A CN 200910074558A CN 101589713 B CN101589713 B CN 101589713B
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Abstract
The invention discloses a cryoprotectant applied to facility vegetables and a preparation method thereof. The cryoprotectant consists of the following components in portion by weight: 1 to 2.5 portions of glucose, 0.03 to 2 portions of polyethylene glycol, and 95.5 to 98.97 portions of distilled water. The preparation method comprises the following steps: taking proper quantities of glucose powder, the polyethylene glycol and the distilled water in proportion; dissolving the glucose and the polyethylene glycol into 10 portions of the distilled water respectively for dilution first; after mixing together, adding the rest distilled water; and mixing the mixture evenly, and standing the product at a temperature of 25 DEG C for 1 hour for use. The vegetable cryoprotectant has the advantages of simple preparation, convenient use, labor and time conservation, low cost and obvious effect; and the raw material components not only are the cryoprotectant but also are nutrient substances for the vegetables, have no toxic hazard to the vegetables and no safety threat to human bodies, and are wide applicable to the production of the facility vegetables in north China. The cryoprotectant can prevent and slow down the loss of the vegetables caused by the low temperature and guarantee the production benefit and the stable income for vegetable growers.
Description
Technical field
The invention belongs to agricultural planting administrative skill field, particularly a kind of anti-cryogenic preparation that is applied to facilities vegetable and preparation method thereof.
Background technology
Low temperature is to influence one of critical limitation factor that China North China facilities vegetable winter-spring season grows.Facilities vegetable often because of cold wave invasion, temperature sharply descends and low temperature poor growth, the physiological activity imbalance of cloudy weather for several days running snow day, change melon, macerate root, even suffer serious damaging to plants caused by sudden drop in temperature and cause enormous economic loss.At present, in facilities vegetable production, take the measure of anti-low temperature to have: kind low temperature resistant, the low light level is adopted in (1); (2) take insulation in the facility, add and gentlely increase measure such as illumination and slow down the influence of low temperature the facilities vegetable growth; (3) adopt suitable field management measure to alleviate the influence of low temperature, as adopt method, the way that adopts many organic fertilizers that bilayer, multilayer cover and adopt drip irrigation technique to reduce and water the water yield at every turn to prevent to reduce measure such as ground temperature to facilities vegetable; (4) adopt the foliage spray growth regulator, change the physiological property of vegetables, strengthen low temperature resistivity.Above-mentioned anti-low temperature measure can be alleviated the harm of low temperature to vegetables to a certain extent, but still exist the cost of investment height, time-consumingly take a lot of work, problem such as effect instability, also lack simple, convenient and swift, obvious results technology for resisting low temperature through product.
Summary of the invention
Deficiency to above-mentioned prior art existence; The object of the invention provides anti-cryogenic preparation of a kind of facilities vegetable and preparation method thereof; Said preparation is sprayed onto the blade face before and after low temperature takes place; Can play prevention and slow down low temperature, solve the problem that the effect that exists in the existing anti-low temperature measure unstablely, time-consuming is taken a lot of work, cost of investment is high the damnous effect of vegetables.
For realizing above-mentioned task, the present invention adopts following technical solution:
A kind of anti-cryogenic preparation that is applied to facilities vegetable, the component of following weight content is formed:
1 part-2.5 parts of glucose
0.03 part-2 parts of polyethylene glycol (PEG400)
95.5 parts-98.97 parts of distilled water
A kind of preparation method who is applied to the anti-cryogenic preparation of facilities vegetable: preparation process is following:
(1) gets 1 part of-2.5 parts of glucose, 0.03 part of-2 parts of polyethylene glycol (PEG400);
(2) get 95.5 parts-98.97 parts distilled water;
(3) earlier with 1 part of-2.5 parts of glucose, 0.03 part of-2 parts of polyethylene glycol are dissolved in respectively in 10 parts the distilled water, stir, more remaining distilled water is added, and mixing, 25 ℃ are placed 1 hour is the cost preparation.
The glucose of one of component of the present invention when being applied to vegetable crop, can improve cell inclusion concentration, reduces freezing point, and the live effect of agent of profit is arranged, and it still be the nutriment of vegetables, is that vegetables prevent and slow down the main protection material of low temperature adverse circumstance.
Polyethylene glycol is applied to vegetable crop as Osmolyte regulator, in seed imbibition germination process, can improve energy-rich compound ATP content and then improve the seed vitality index; Can improve the seedling chlorophyll content, it is active to increase protective enzyme, improves crop cold resistance and drought resistance.Zhang Chunmei research is pointed out; Under the drought stress of Polyethylene Glycol Simulated; Abscisic acid (ABA) and the equal run-up of jasmonic (JA) content in 2 kind tomato seedling roots and the leaf; Free state spermidine (fSpd), spermine (fSpm), putrescine (fPut) content and PAO (PAO) activity all rise earlier, descend after reaching peak value during 12h, and the ability that accumulates ABA, JA and Spd, Spm in the kind seedling root that drought resistance is strong and the leaf obviously is better than the kind a little less than the drought resistance.The rising of ABA and JA content is induced in the accumulation of endogenous polyamines, thereby causes the drought resistance of tomato seedling to strengthen.(Zhang Chunmei.The variation of ABA, JA and polyamine content and polyamine oxidase enzymic activity in the tomato seedling under polyethylene glycol is coerced.Plant Physiology Communications, in August, 2008,689-692.)
The method for using of vegetable cryoprotective agent of the present invention is: foliage-spray is 1-2 time before and after seedling phase and low temperature arriving, and consumption is as the criterion to squirt the blade face.
Range of application: facilities vegetable such as tomato, cucumber, custard squash, capsicum, eggplant etc.
Result of the test shows, before seedling phase and low temperature season arriving, sprays, and can significantly improve the activity of POD enzyme, reduces the activity of SOD enzyme, effectively reduces the index that damages to plants caused by sudden drop in temperature of facilities vegetable.
Damage to plants caused by sudden drop in temperature index and be meant the injured leaf area size of different vegetable under low temperature stress is divided into 1,2,3,4 different stage, 1 grade is the lightest, and 4 grades are the heaviest, calculate with following formula:
Damage to plants caused by sudden drop in temperature index=(1 * 1 grade blade number+2 * 2 grade blade numbers+3 * 3 grade blade numbers+4 * 4 grade blade numbers)/(total strain number * every strain number of blade * 4) * 100 parts
Vegetable cryoprotective agent of the present invention, preparation is simple, and is easy to use, saving of work and time; With low cost, effect is obvious, raw material components be cryoprotective agent be again the vegetable nutrient material; Vegetables are not poisoned, human body is not had security threat, produce adaptable across the northern China facilities vegetable.The present invention can prevent and slow down the loss that low temperature causes vegetables, ensures vegetable grower's productivity effect and stable income.
Embodiment
Embodiment 1
Blue or green generation custard squash early, water seed soaking vernalization on July 14 in 2008,9cm * 9cm feeding block seedlings raising; One heart stage of three leaves, spray the mixed liquor of 2.50 parts of glucose, 0.03 part of polyethylene glycol and 97.47 parts of distilled water; Contrast is handled with clear water, and second day immigration climatic cabinate carries out low temperature stress to be handled, and temperature is 6 ℃; Temperature photoperiod 10h (daytime)/14h (night), handle measure seedling leaves behind the 3d damage to plants caused by sudden drop in temperature the exponential sum physical signs.The material of participating in the experiment in every processing is 8 strains, repeats 3 times, adopts the DPS statistical method that data are carried out statistical analysis, and result of the test shows that damaging to plants caused by sudden drop in temperature index is 21.38%, for contrasting 71.25%; The POD enzymic activity is 354.28U/gmin, is 158.59% of contrast, and the SOD enzymic activity is 416.86U/gmin, is 68.26% of contrast.
Embodiment 2
Blue or green generation custard squash early, water seed soaking vernalization on July 14 in 2008,9cm * 9cm feeding block seedlings raising; One heart stage of three leaves, spray the mixed liquor of 2.0 parts of glucose, 0.1 part of polyethylene glycol and 97.9 parts of distilled water; Contrast is handled with clear water, and second day immigration climatic cabinate carries out low temperature stress to be handled, and temperature is 6 ℃; Temperature photoperiod 10h (daytime)/14h (night) handles the index that damages to plants caused by sudden drop in temperature of measuring seedling leaves behind the 3d.The material of participating in the experiment in every processing is 8 strains, repeats 3 times, adopts the DPS statistical method that data are carried out statistical analysis, and result of the test shows that damaging to plants caused by sudden drop in temperature index is 22.61%, for contrasting 75.36%; The POD enzymic activity is 402.69U/gmin, is 180.26% of contrast, and the SOD enzymic activity is 463.15U/gmin, is 75.84% of contrast;
Embodiment 3
The anti-king's custard squash of heat, water seed soaking vernalization on July 14 in 2008,9cm * 9cm feeding block seedlings raising; One heart stage of three leaves, spray the mixed liquor of 1.5 parts of glucose, 0.5 part of polyethylene glycol and 98.0 parts of distilled water; Contrast is handled with clear water, and second day immigration climatic cabinate carries out low temperature stress to be handled, and temperature is 6 ℃; Temperature photoperiod 10h (daytime)/14h (night), handle measure seedling leaves behind the 3d damage to plants caused by sudden drop in temperature the exponential sum physical signs.The material of participating in the experiment in every processing is 8 strains, repeats 3 times, adopts the DPS statistical method that data are carried out statistical analysis, and result of the test shows that damaging to plants caused by sudden drop in temperature index is 19.39%, is 78.29% of contrast; The POD enzymic activity is 381.75U/gmin, is 144.24% of contrast, and the SOD enzymic activity is 396.83U/gmin, is 64.98% of contrast.
Embodiment 4
The anti-king's custard squash of heat, water seed soaking vernalization on July 14 in 2008,9cm * 9cm feeding block seedlings raising; One heart stage of three leaves, spray the mixed liquor of 1.0 parts of glucose, 1.5 parts of polyethylene glycol and 97.5 parts of distilled water; Contrast is handled with clear water, and second day immigration climatic cabinate carries out low temperature stress to be handled, and temperature is 6 ℃; Temperature photoperiod 10h (daytime)/14h (night) handles the index that damages to plants caused by sudden drop in temperature of measuring seedling leaves behind the 3d.The material of participating in the experiment in every processing is 8 strains, repeats 3 times, adopts the DPS statistical method that data are carried out statistical analysis, and result of the test shows that damaging to plants caused by sudden drop in temperature index is 18.29%, is 73.85% of contrast; The POD enzymic activity is 431.00U/gmin, is 162.85% of contrast, and the SOD enzymic activity is 442.02U/gmin, is 72.38% of contrast.
Embodiment 5
Shanxi tomato No. 4; On August 6th, 2008 point disk seedling growing, 1 heart stage of 4 leaves, spray the mixed liquor of 1.0 parts of glucose, 0.15 part of polyethylene glycol and 98.85 parts of distilled water, contrast is handled with clear water; Second day immigration climatic cabinate carries out low temperature stress to be handled; Temperature is 6 ℃, temperature photoperiod 10h (daytime)/14h (night), handle measure seedling leaves behind the 10d damage to plants caused by sudden drop in temperature the exponential sum physical signs.The material of participating in the experiment in every processing is 12 strains, repeats 3 times, adopts the DPS statistical method that data are carried out statistical analysis.Result of the test shows that damaging to plants caused by sudden drop in temperature index is 29.21%, is 80.97% of contrast; The POD enzymic activity is 589.20U/gmin, is 179.15% of contrast, and the SOD enzymic activity is 382.47U/gmin, is 68.28% of contrast.
Embodiment 6
With excellent No. 31 cucumber in Tianjin, on August 6th, 2008, presoaking and germinating was seeded in during 8cm * 8cm nutrition broadcasts; One heart stage of two leaves, spray the mixed liquor of 1.5 parts of glucose, 0.5 part of polyethylene glycol and 98.0 parts of distilled water; With the clear water is contrast, and second day immigration climatic cabinate carries out low temperature stress to be handled, and temperature is 6 ℃; Temperature is 10h (daytime)/14h (night) photoperiod, and that handles 5d " Invest, Then Investigate " seedling leaves damages to plants caused by sudden drop in temperature the exponential sum physical signs.The material of participating in the experiment in every processing is 8 strains, repeats 3 times, adopts the DPS statistical method that data are carried out statistical analysis.Result of the test shows that damaging to plants caused by sudden drop in temperature index is 18.29%, is 73.85% of contrast; The POD enzymic activity is 386.66U/gmin, is 147.59% of contrast, and the SOD enzymic activity is 428.88U/gmin, is 63.54% of contrast.
Embodiment 7
No. 1 capsicum of Shanxi point green pepper; On August 6th, 2008 point disk seedling growing, 1 heart stage of 4 leaves, spray the mixed liquor of 2.50 parts of glucose, 0.03 part of polyethylene glycol and 97.47 parts of distilled water, is contrast with the clear water; Second day immigration climatic cabinate carries out low temperature stress to be handled; Temperature is 6 ℃, temperature photoperiod 10h (daytime)/14h (night), and that handles 10d " Invest, Then Investigate " seedling leaves damages to plants caused by sudden drop in temperature the exponential sum physical signs.The material of participating in the experiment in every processing is 8 strains, repeats 3 times, adopts the DPS statistical method that data are carried out statistical analysis.Result of the test shows that damaging to plants caused by sudden drop in temperature index is 25.24%, is 81.59% of contrast.The POD enzymic activity is 372.28U/gmin, is 149.99% of contrast, and the SOD enzymic activity is 462.35U/gmin, is 77.86% of contrast.
Embodiment 8
The Shanxi purple long eggplant; On June 6th, 2008 feeding block seedlings raising, 1 heart stage of 3 leaves, spray the mixed liquor of 1.0 parts of glucose, 2.0 parts of polyethylene glycol and 97.0 parts of distilled water, is contrast with the clear water; Second day immigration climatic cabinate carries out low temperature stress to be handled; Temperature is 6 ℃, and temperature photoperiod 10h (daytime)/14h (night) handles the index that damages to plants caused by sudden drop in temperature of 7d " Invest, Then Investigate " seedling leaves.The material of participating in the experiment in every processing is 8 strains, repeats 3 times, adopts the DPS statistical method that data are carried out statistical analysis.Result of the test shows that damaging to plants caused by sudden drop in temperature index is 34.34%, is 75.68% of contrast.
Embodiment 9
Blue or green generation custard squash early, on September 24th, 2008 was seeded in the greenhouse, and the field planting seeding row spacing is 5cm * 35cm.If 1 processing, 1 contrast, 3 repetitions whenever are treated to 35 strains.Handle the mixed liquor that one heart stage of three leaves sprays 1.0 parts of glucose, 0.1 part of polyethylene glycol and 98.9 parts of distilled water for the 1st time, spray same cryoprotective agent the 2nd time after 7 days.Threw off the canopy film on October 28 and carry out the natural low temperature processing, damaging to plants caused by sudden drop in temperature appears in October 31, and lid upper greenhouse (temperature is-2 ℃-22 ℃ according to surveying and determination) is measured physical signs immediately, and November 2, index was damaged to plants caused by sudden drop in temperature in investigation.Result of the test shows that the POD enzymic activity is 382.59U/gmin, is 168.52% of contrast, and the SOD enzymic activity is 485.28U/gmin, is 78.29% of contrast; Damaging to plants caused by sudden drop in temperature index is 28.28%, is 69.52% of contrast.
Embodiment 10
Excellent No. 31 cucumber in Tianjin, on September 18th, 2008 presoaking and germinating, 8cm * 8cm feeding block seedlings raising, one heart stage of two leaves, be colonizated in the greenhouse, seeding row spacing is 5cm * 35cm.If 1 processing, 1 contrast, 3 repetitions whenever are treated to 35 strains.Post processing in 7 days sprays the mixed liquor of 1.5 parts of glucose, 1.0% polyethylene glycol and 97.5 parts of distilled water for the 1st time, sprays same cryoprotective agent the 2nd time after 14 days; Contrast spray equivalent clear water.Threw off the canopy film on October 28 and carry out the natural low temperature processing, damaging to plants caused by sudden drop in temperature appears in October 31, lid upper greenhouse (temperature is-2 ℃-22 ℃ according to surveying and determination).Measure physical signs immediately, November 2, index was damaged to plants caused by sudden drop in temperature in investigation.Result of the test shows that the POD enzymic activity is 369.52U/gmin, is 166.62% of contrast, and the SOD enzymic activity is 428.27U/gmin, is 67.35% of contrast, and damaging to plants caused by sudden drop in temperature index is 28.52%, is 73.29% of contrast.
Claims (2)
1. anti-cryogenic preparation that is applied to facilities vegetable, it is characterized in that: the component by following weight content is formed:
1 part-2.5 parts of glucose
0.03 part-2 parts of polyethylene glycol
95.5 parts-98.97 parts of distilled water
Wherein said polyethylene glycol is selected from PEG400.
2. according to the described a kind of preparation method who is applied to the anti-cryogenic preparation of facilities vegetable of claim 1, it is characterized in that: preparation process is following:
(1) gets 1 part of-2.5 parts of glucose, 0.03 part of-2 parts of polyethylene glycol;
(2) get 95.5 parts-98.97 parts distilled water;
(3) earlier with 1 part of-2.5 parts of glucose, 0.03 part of-2 parts of polyethylene glycol are dissolved in respectively in 10 parts the distilled water, mix, more remaining distilled water is added, and mixing, 25 ℃ are placed 1 hour is the cost preparation;
Wherein said polyethylene glycol is selected from PEG400.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2009100745583A CN101589713B (en) | 2009-06-24 | 2009-06-24 | Cryoprotectant applied to facility vegetables and preparation method thereof |
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| CN2009100745583A CN101589713B (en) | 2009-06-24 | 2009-06-24 | Cryoprotectant applied to facility vegetables and preparation method thereof |
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| CN101589713B true CN101589713B (en) | 2012-05-09 |
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101785476B (en) * | 2010-02-08 | 2012-08-01 | 山西省农业科学院蔬菜研究所 | Cryoprotective agent for protected vegetable and preparation method thereof |
| CN101779670B (en) * | 2010-02-08 | 2012-10-03 | 山西省农业科学院作物遗传研究所 | Low-temperature-resistance growth substance applied to corns and preparation method thereof |
| CN102845424A (en) * | 2012-08-28 | 2013-01-02 | 江苏徐淮地区淮阴农业科学研究所 | Low temperature resistant regulator for sheltered cucumber and preparation method thereof |
| CN104430642A (en) * | 2014-12-09 | 2015-03-25 | 镇江拜因诺生物科技有限公司 | Low-temperature protective agent for vegetables |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1090970A (en) * | 1993-02-17 | 1994-08-24 | 陕西师范大学 | plant cryoprotectant |
| CN1922995A (en) * | 2005-08-31 | 2007-03-07 | 青岛海联植物科技研究所 | Technique of improving maize cold resistant and moisture proof character by using cryoprotectant |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1090970A (en) * | 1993-02-17 | 1994-08-24 | 陕西师范大学 | plant cryoprotectant |
| CN1922995A (en) * | 2005-08-31 | 2007-03-07 | 青岛海联植物科技研究所 | Technique of improving maize cold resistant and moisture proof character by using cryoprotectant |
Non-Patent Citations (3)
| Title |
|---|
| JP特开平6-271897A 1994.09.27 |
| Kadon K. Hintz, et al..Phytoestrogenic Isoflavones Daidzein and Genistein Reduce Glucose-Toxicity-Induced Cardiac Contractile Dysfunction in Ventricular Myocytes.《ENDOCRINE RESEARCH》.2004,第30卷(第2期),第215-223页. * |
| 樊建军,等.棚室蔬菜低温障害的预防及补救.《甘肃农业科技》.2008,(第11期),第61-62页. * |
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