CN1090970A - plant cryoprotectant - Google Patents
plant cryoprotectant Download PDFInfo
- Publication number
- CN1090970A CN1090970A CN 93101482 CN93101482A CN1090970A CN 1090970 A CN1090970 A CN 1090970A CN 93101482 CN93101482 CN 93101482 CN 93101482 A CN93101482 A CN 93101482A CN 1090970 A CN1090970 A CN 1090970A
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- China
- Prior art keywords
- cyclodextrin
- plant
- beta
- low temperature
- gamma
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 239000002577 cryoprotective agent Substances 0.000 title abstract 2
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 claims abstract description 26
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims abstract description 15
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims abstract description 14
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 claims abstract description 14
- 239000002202 Polyethylene glycol Substances 0.000 claims abstract description 9
- 229920001223 polyethylene glycol Polymers 0.000 claims abstract description 9
- 239000005648 plant growth regulator Substances 0.000 claims abstract description 8
- 229920001817 Agar Polymers 0.000 claims abstract description 7
- 108010010803 Gelatin Proteins 0.000 claims abstract description 7
- 229930182555 Penicillin Natural products 0.000 claims abstract description 7
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 claims abstract description 7
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims abstract description 7
- 229930006000 Sucrose Natural products 0.000 claims abstract description 7
- 235000010419 agar Nutrition 0.000 claims abstract description 7
- DNJIEGIFACGWOD-UHFFFAOYSA-N ethyl mercaptane Natural products CCS DNJIEGIFACGWOD-UHFFFAOYSA-N 0.000 claims abstract description 7
- 239000008273 gelatin Substances 0.000 claims abstract description 7
- 229920000159 gelatin Polymers 0.000 claims abstract description 7
- 235000019322 gelatine Nutrition 0.000 claims abstract description 7
- 235000011852 gelatine desserts Nutrition 0.000 claims abstract description 7
- 235000011187 glycerol Nutrition 0.000 claims abstract description 7
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims abstract description 7
- 235000019341 magnesium sulphate Nutrition 0.000 claims abstract description 7
- 235000019796 monopotassium phosphate Nutrition 0.000 claims abstract description 7
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims abstract description 7
- 229940049954 penicillin Drugs 0.000 claims abstract description 7
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims abstract description 7
- 239000003375 plant hormone Substances 0.000 claims abstract description 7
- 235000010333 potassium nitrate Nutrition 0.000 claims abstract description 7
- 239000004323 potassium nitrate Substances 0.000 claims abstract description 7
- 239000005720 sucrose Substances 0.000 claims abstract description 7
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 claims abstract description 7
- 239000004375 Dextrin Substances 0.000 claims abstract description 6
- 229920001353 Dextrin Polymers 0.000 claims abstract description 6
- 235000019425 dextrin Nutrition 0.000 claims abstract description 6
- JLIDBLDQVAYHNE-YKALOCIXSA-N (+)-Abscisic acid Chemical compound OC(=O)/C=C(/C)\C=C\[C@@]1(O)C(C)=CC(=O)CC1(C)C JLIDBLDQVAYHNE-YKALOCIXSA-N 0.000 claims description 16
- 229920001450 Alpha-Cyclodextrin Polymers 0.000 claims description 13
- 229920000858 Cyclodextrin Polymers 0.000 claims description 13
- HFHDHCJBZVLPGP-RWMJIURBSA-N alpha-cyclodextrin Chemical compound OC[C@H]([C@H]([C@@H]([C@H]1O)O)O[C@H]2O[C@@H]([C@@H](O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O3)[C@H](O)[C@H]2O)CO)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@@H]3O[C@@H]1CO HFHDHCJBZVLPGP-RWMJIURBSA-N 0.000 claims description 13
- 229940043377 alpha-cyclodextrin Drugs 0.000 claims description 13
- GDSRMADSINPKSL-HSEONFRVSA-N gamma-cyclodextrin Chemical compound OC[C@H]([C@H]([C@@H]([C@H]1O)O)O[C@H]2O[C@@H]([C@@H](O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O3)[C@H](O)[C@H]2O)CO)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@@H]3O[C@@H]1CO GDSRMADSINPKSL-HSEONFRVSA-N 0.000 claims description 13
- 229940080345 gamma-cyclodextrin Drugs 0.000 claims description 13
- IXVMHGVQKLDRKH-YEJCTVDLSA-N (22s,23s)-epibrassinolide Chemical compound C1OC(=O)[C@H]2C[C@H](O)[C@H](O)C[C@]2(C)[C@H]2CC[C@]3(C)[C@@H]([C@H](C)[C@H](O)[C@@H](O)[C@H](C)C(C)C)CC[C@H]3[C@@H]21 IXVMHGVQKLDRKH-YEJCTVDLSA-N 0.000 claims description 10
- IXVMHGVQKLDRKH-VRESXRICSA-N Brassinolide Natural products O=C1OC[C@@H]2[C@@H]3[C@@](C)([C@H]([C@@H]([C@@H](O)[C@H](O)[C@H](C(C)C)C)C)CC3)CC[C@@H]2[C@]2(C)[C@@H]1C[C@H](O)[C@H](O)C2 IXVMHGVQKLDRKH-VRESXRICSA-N 0.000 claims description 10
- UDPGUMQDCGORJQ-UHFFFAOYSA-N (2-chloroethyl)phosphonic acid Chemical group OP(O)(=O)CCCl UDPGUMQDCGORJQ-UHFFFAOYSA-N 0.000 claims description 9
- 239000000203 mixture Substances 0.000 claims description 9
- -1 steroid lactone Chemical class 0.000 claims description 9
- FCRACOPGPMPSHN-UHFFFAOYSA-N desoxyabscisic acid Natural products OC(=O)C=C(C)C=CC1C(C)=CC(=O)CC1(C)C FCRACOPGPMPSHN-UHFFFAOYSA-N 0.000 claims description 8
- RMOGWMIKYWRTKW-UONOGXRCSA-N (S,S)-paclobutrazol Chemical compound C([C@@H]([C@@H](O)C(C)(C)C)N1N=CN=C1)C1=CC=C(Cl)C=C1 RMOGWMIKYWRTKW-UONOGXRCSA-N 0.000 claims description 7
- 239000005985 Paclobutrazol Substances 0.000 claims description 7
- 239000005974 Chlormequat Substances 0.000 claims description 6
- 235000008733 Citrus aurantifolia Nutrition 0.000 claims description 6
- 229910002651 NO3 Inorganic materials 0.000 claims description 6
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 claims description 6
- 235000011941 Tilia x europaea Nutrition 0.000 claims description 6
- JUZXDNPBRPUIOR-UHFFFAOYSA-N chlormequat Chemical compound C[N+](C)(C)CCCl JUZXDNPBRPUIOR-UHFFFAOYSA-N 0.000 claims description 6
- 239000004571 lime Substances 0.000 claims description 6
- 241000206672 Gelidium Species 0.000 claims description 4
- 239000002253 acid Substances 0.000 claims 1
- 241000196324 Embryophyta Species 0.000 abstract description 14
- 235000007164 Oryza sativa Nutrition 0.000 abstract description 10
- 235000009566 rice Nutrition 0.000 abstract description 10
- 239000003795 chemical substances by application Substances 0.000 abstract description 6
- 244000241235 Citrullus lanatus Species 0.000 abstract description 3
- 235000012828 Citrullus lanatus var citroides Nutrition 0.000 abstract description 3
- 244000061176 Nicotiana tabacum Species 0.000 abstract description 3
- 235000002637 Nicotiana tabacum Nutrition 0.000 abstract description 3
- 239000008272 agar Substances 0.000 abstract description 3
- 235000013311 vegetables Nutrition 0.000 abstract description 3
- 229920000742 Cotton Polymers 0.000 abstract description 2
- 238000002156 mixing Methods 0.000 abstract description 2
- ZCCIPPOKBCJFDN-UHFFFAOYSA-N calcium nitrate Chemical compound [Ca+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O ZCCIPPOKBCJFDN-UHFFFAOYSA-N 0.000 abstract 2
- PWVXXGRKLHYWKM-UHFFFAOYSA-N 5-[2-(benzenesulfonyl)ethyl]-3-[(1-methylpyrrolidin-2-yl)methyl]-1h-indole Chemical compound CN1CCCC1CC(C1=C2)=CNC1=CC=C2CCS(=O)(=O)C1=CC=CC=C1 PWVXXGRKLHYWKM-UHFFFAOYSA-N 0.000 abstract 1
- 240000007594 Oryza sativa Species 0.000 abstract 1
- 241000209094 Oryza Species 0.000 description 9
- 239000000243 solution Substances 0.000 description 8
- 235000011837 pasties Nutrition 0.000 description 5
- 239000007864 aqueous solution Substances 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 238000005303 weighing Methods 0.000 description 4
- 238000002791 soaking Methods 0.000 description 3
- 238000001035 drying Methods 0.000 description 2
- 239000007921 spray Substances 0.000 description 2
- 238000005507 spraying Methods 0.000 description 2
- 229910001220 stainless steel Inorganic materials 0.000 description 2
- 239000010935 stainless steel Substances 0.000 description 2
- 238000010998 test method Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 206010013786 Dry skin Diseases 0.000 description 1
- 244000037666 field crops Species 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 150000002596 lactones Chemical class 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
A plant cryoprotectant is prepared from calcium nitrate, potassium nitrate, magnesium sulfate, potassium dihydrogen phosphate, glycerol, agar, sucrose, polyethylene glycol with molecular weight of 6000, chlormequat chloride, penicillin, dimethyl sulfoxide, mercaptoethanol, gelatin, dextrin, plant hormone and plant growth regulator by mixing. The low-temperature cold-resistant agent can resist the low temperature of-2 to-5 ℃ for 10 hours, and is suitable for seedling culture of crops such as cotton, rape, vegetables, rice, flue-cured tobacco, watermelon and the like.
Description
The invention belongs to the plant growth regulator technical field, relate in particular to the plant low temperature protectant of forming by mixing such as dextrin, plant hormone, plant growth regulator and mineral salt, polysaccharide.
The rice seed soaking cold-resistance agent of Xiao Shouing in the market, territory of use is on the south the Changjiang river, be mainly used in and soak rice paddy seed when rice seedling is cultivated, the rice paddy seed that this cold-resistance agent of process soaked, can only resist the low temperature more than 0 ℃, can not resist the low temperature below 0 ℃, this cold-resistance agent can not use in the seedling cultivation process of crops such as vegetables, watermelon, tobacco with the area, north temperate zone in the Changjiang river.
The objective of the invention is to overcome the shortcoming of above-mentioned rice seed soaking cold-resistance agent, a kind of plant low temperature protectant that the region is big, purposes is wide that uses is provided.
For achieving the above object, the scheme that the present invention adopts is: with nitrate of lime, potassium nitrate, magnesium sulfate, potassium dihydrogen phosphate, glycerine, agar, sucrose, molecular weight is that 6000 polyethylene glycol, chlormequat, penicillin, dimethyl sulfoxide (DMSO), mercaptoethanol, gelatin, dextrin, plant hormone and plant growth regulator are mixed and made into.
Dextrin in the mixed composition of the present invention is alpha-cyclodextrin or beta-schardinger dextrin-or gamma-cyclodextrin, and plant hormone is abscisic acid, brassinosteroid or courage steroid lactone, and plant growth regulator is ethrel or paclobutrazol.
In mixed composition of the present invention, the weight ratio of alpha-cyclodextrin or beta-schardinger dextrin-or gamma-cyclodextrin and abscisic acid is 1: 0.000264～0.00264, the weight ratio of alpha-cyclodextrin or beta-schardinger dextrin-or gamma-cyclodextrin and brassinosteroid or courage steroid lactone is 1: 0.000001～0.00001, and the weight ratio of alpha-cyclodextrin or beta-schardinger dextrin-or gamma-cyclodextrin and ethrel or paclobutrazol is 1: 0.006～0.01.In mixed composition of the present invention, the weight ratio of alpha-cyclodextrin or beta-schardinger dextrin-or gamma-cyclodextrin and other component of the present invention is: with the weight ratio of nitrate of lime be 1: 0.08, be respectively 1: 0.02 with the weight ratio of potassium nitrate, magnesium sulfate, potassium dihydrogen phosphate, with glycerine, sucrose, agar, molecular weight be that the weight ratio of 6000 polyethylene glycol, mercaptoethanol, dimethyl sulfoxide (DMSO), gelatin was respectively 1: 0.01, with the weight ratio of chlormequat be 1: 0.003, with the weight ratio of penicillin (800,000 unit) be 1: 0.1.
For making this plant low temperature protectant, can adopt following method.
1, takes by weighing
Take by weighing alpha-cyclodextrin or beta-schardinger dextrin-or gamma-cyclodextrin, abscisic acid, brassinosteroid or courage steroid lactone, ethrel or paclobutrazol, nitrate of lime, potassium nitrate, magnesium sulfate, potassium dihydrogen phosphate, glycerine, sucrose, agar-agar, molecular weight in proportion and be 6000 polyethylene glycol, chlormequat, penicillin, dimethyl sulfoxide (DMSO), mercaptoethanol, gelatin.
2, mix
The alpha-cyclodextrin that taken by weighing or beta-schardinger dextrin-or gamma-cyclodextrin are poured in the molten device, and the water that adds 2 times stirs at normal temperatures and pressures with the stainless steel agitator, makes it become pasty state.Be that 6000 polyethylene glycol, abscisic acid, brassinosteroid or courage steroid lactone are put into three molten devices respectively again with molecular weight, add ethanolic solution then respectively till the dissolving fully, be mixed with molecular weight and be 6000 polyethylene glycol ethanolic solution, abscisic acid ethanolic solution, brassinosteroid or courage steroid lactone ethanolic solution.
With the nitrate of lime, potassium nitrate, magnesium sulfate, potassium dihydrogen phosphate, glycerine, agar-agar, sucrose, penicillin (800,000 unit), dimethyl sulfoxide (DMSO), mercaptoethanol, gelatin, chlormequat, ethrel or the paclobutrazol that have taken by weighing and the molecular weight that has prepared 6000 polyethylene glycol ethanolic solution, abscisic acid ethanolic solution, brassinosteroid or courage steroid lactone ethanolic solution, pour alpha-cyclodextrin or the beta-schardinger dextrin-or the gamma-cyclodextrin of the pasty state that has prepared respectively into, stirred 2 hours with the stainless steel agitator, it is fully mixed.Make the plant low temperature protectant of pasty state.
3, drying
With the plant low temperature protectant of Manufactured pasty state under the low temperature situation dry or with warm air drier at 30 ℃～40 ℃ low temperature dryings.The plant low temperature protectant of pasty state just condenses into bulk.
4, pulverize
Become powder with making block plant low temperature protectant with grinder or ball mill grinding.
The present invention is through laboratory and small size field trial, and result of the test shows: the present invention compares with the rice seed soaking cold-resistance agent, has advantages such as usable floor area is big, applicable crops is many, cold-resistant temperature is low, can promote the use of with the area, north temperate zone in the Changjiang river.
The inventor has provided a most preferred embodiment of the present invention.
In mixed composition of the present invention, alpha-cyclodextrin or beta-schardinger dextrin-or gamma-cyclodextrin select for use the rape lactone of beta-schardinger dextrin-, plant hormone or courage steroid lactone to select brassinosteroid for use, and the ethrel or the paclobutrazol of plant growth regulator are selected ethrel for use.Present embodiment mixes in following ratio.
The weight ratio of beta-schardinger dextrin-and abscisic acid is 1: 0.000264, and the weight ratio of beta-schardinger dextrin-and brassinosteroid is 1: 0.000001, and the weight ratio of beta-schardinger dextrin-and ethrel is 1: 0.006.In mixed composition, beta-schardinger dextrin-and other component of the present invention, nitrate of lime, potassium nitrate, magnesium sulfate, potassium dihydrogen phosphate, glycerine, sucrose, agar-agar, molecular weight be 6000 polyethylene glycol, mercaptoethanol, dimethyl sulfoxide (DMSO), gelatin, chlormequat, penicillin weight ratio as hereinbefore.
The plant low temperature protectant that the inventor adopts the mixed proportion of present embodiment to make is tested in laboratory and field.
1, the preparation of plant low temperature protectant solution
Manufactured plant low temperature protectant is put into molten device, and the water that adds 98.75: 1 temperature and be 50 ℃ dissolves it fully, is mixed with the plant low temperature protectant aqueous solution.
2, test method
The plant low temperature protectant aqueous solution that has prepared with the sprayer spraying at the beds of growing seedlings of crops such as cotton, rape, vegetables, paddy rice, flue-cured tobacco, watermelon at late frost in spring previous month (concrete time can determining) sooner or later according to the climatic variation of locality, late frost; sprayed once every ten days; each use metering is 2 kilograms; the aqueous solution of the present invention can spray 1 mu of rice shoot; spray altogether three times, sprayed after 10 days rice shoot for the third time and can be transplanted to the land for growing field crops.
3, result of the test
Behind the aqueous solution of thermophilous through spraying three the present invention, its cold tolerance is brought up to-5 ℃ from-2 ℃, lasts 10 hours.
Using method of the present invention is identical with test method with the use metering.
Claims (3)
1, a kind of plant low temperature protectant; include in its mixed composition: nitrate of lime, potassium nitrate, magnesium sulfate, potassium dihydrogen phosphate, glycerine, agar-agar, sucrose, molecular weight are 6000 polyethylene glycol, chlormequat, penicillin, dimethyl sulfoxide (DMSO), mercaptoethanol, gelatin, it is characterized in that also including in its mixed composition: dextrin, plant hormone and plant growth regulator.
2, according to the described plant low temperature protectant of claim 1; it is characterized in that: said dextrin is alpha-cyclodextrin or beta-schardinger dextrin-or gamma-cyclodextrin; said plant hormone is the acid that falls, brassinosteroid or courage steroid lactone, and said plant growth regulator is ethrel or paclobutrazol.
3, according to claim 1 or 2 described plant low temperature protectants; it is characterized in that: in its mixed composition; the weight ratio of alpha-cyclodextrin or beta-schardinger dextrin-or gamma-cyclodextrin and abscisic acid is 1: 0.000264～0.00264; the weight ratio of alpha-cyclodextrin or beta-schardinger dextrin-or gamma-cyclodextrin and brassinosteroid or courage steroid lactone is 1: 0.000001～0.00001, and the weight ratio of alpha-cyclodextrin or beta-schardinger dextrin-or gamma-cyclodextrin and ethrel paclobutrazol is 1: 0.006～0.01.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN93101482A CN1044846C (en) | 1993-02-17 | 1993-02-17 | plant cryoprotectant |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN93101482A CN1044846C (en) | 1993-02-17 | 1993-02-17 | plant cryoprotectant |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1090970A true CN1090970A (en) | 1994-08-24 |
| CN1044846C CN1044846C (en) | 1999-09-01 |
Family
ID=4983612
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN93101482A Expired - Fee Related CN1044846C (en) | 1993-02-17 | 1993-02-17 | plant cryoprotectant |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1044846C (en) |
Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6455468B1 (en) | 2000-04-28 | 2002-09-24 | Regents Of The University Of Minnesota | Seed treatment composition |
| CN1100736C (en) * | 1999-10-29 | 2003-02-05 | 杨凌光泰果树高新技术开发有限公司 | Pear tree nutrient injection and preparation method thereof |
| CN101589713B (en) * | 2009-06-24 | 2012-05-09 | 山西省农业科学院蔬菜研究所 | Cryoprotectant applied to facility vegetables and preparation method thereof |
| CN102515948A (en) * | 2011-12-09 | 2012-06-27 | 大千生态景观股份有限公司 | Plant antifreezer and its preparation method |
| CN102630703A (en) * | 2011-02-11 | 2012-08-15 | 谭汉卿 | Fruit tree disease and freeze preventing agent |
| CN103570442A (en) * | 2012-08-03 | 2014-02-12 | 天津春之韵园艺工程技术发展有限公司 | Formula of antifreezing agent for vertical greening plants and preparation method of antifreezing agent |
| CN103621514A (en) * | 2013-12-06 | 2014-03-12 | 中国农业科学院茶叶研究所 | Tea tree anti-freezing agent and preparation method thereof |
| CN104430642A (en) * | 2014-12-09 | 2015-03-25 | 镇江拜因诺生物科技有限公司 | Low-temperature protective agent for vegetables |
| CN104798788A (en) * | 2015-03-16 | 2015-07-29 | 甘肃农业大学 | Cold-resistant agent for brassica rapa L. seedlings and application method of cold-resistant agent |
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-
1993
- 1993-02-17 CN CN93101482A patent/CN1044846C/en not_active Expired - Fee Related
Cited By (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1100736C (en) * | 1999-10-29 | 2003-02-05 | 杨凌光泰果树高新技术开发有限公司 | Pear tree nutrient injection and preparation method thereof |
| US6455468B1 (en) | 2000-04-28 | 2002-09-24 | Regents Of The University Of Minnesota | Seed treatment composition |
| CN101589713B (en) * | 2009-06-24 | 2012-05-09 | 山西省农业科学院蔬菜研究所 | Cryoprotectant applied to facility vegetables and preparation method thereof |
| CN102630703A (en) * | 2011-02-11 | 2012-08-15 | 谭汉卿 | Fruit tree disease and freeze preventing agent |
| CN102515948A (en) * | 2011-12-09 | 2012-06-27 | 大千生态景观股份有限公司 | Plant antifreezer and its preparation method |
| CN102515948B (en) * | 2011-12-09 | 2013-12-11 | 大千生态景观股份有限公司 | Plant antifreezer and its preparation method |
| CN103570442A (en) * | 2012-08-03 | 2014-02-12 | 天津春之韵园艺工程技术发展有限公司 | Formula of antifreezing agent for vertical greening plants and preparation method of antifreezing agent |
| CN103621514A (en) * | 2013-12-06 | 2014-03-12 | 中国农业科学院茶叶研究所 | Tea tree anti-freezing agent and preparation method thereof |
| CN104430642A (en) * | 2014-12-09 | 2015-03-25 | 镇江拜因诺生物科技有限公司 | Low-temperature protective agent for vegetables |
| CN104798788A (en) * | 2015-03-16 | 2015-07-29 | 甘肃农业大学 | Cold-resistant agent for brassica rapa L. seedlings and application method of cold-resistant agent |
| CN104798788B (en) * | 2015-03-16 | 2017-02-22 | 甘肃农业大学 | Cold-resistant agent for brassica rapa L. seedlings and application method of cold-resistant agent |
| CN105272612A (en) * | 2015-10-27 | 2016-01-27 | 丹阳市香逸林果专业合作社 | Anti-freezing nutrient solution for crape myrtle |
| CN105794842A (en) * | 2016-03-31 | 2016-07-27 | 郑龙 | Anti-freezing agent for crops |
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| CN105766936B (en) * | 2016-04-14 | 2018-06-26 | 江苏徐淮地区淮阴农业科学研究所 | Cold-resistant agent for improving low-temperature resistance of hot pepper |
| CN106172517A (en) * | 2016-07-29 | 2016-12-07 | 安徽徽王农业有限公司 | Freeze proof harmful medicament for blue berry and its preparation method and application |
| CN109090135A (en) * | 2018-08-16 | 2018-12-28 | 江苏农林职业技术学院 | Exogenous Silicon cold-resistance agent and the preparation method and application thereof |
| CN110050784A (en) * | 2019-05-23 | 2019-07-26 | 南京林业大学 | Anti- pine nematode masson pine embryo callus cryopreservation method |
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