CN101778952B - 使用寡核苷酸和显著缺乏5’-3’核酸外切酶活性的聚合酶抑制扩增 - Google Patents

使用寡核苷酸和显著缺乏5’-3’核酸外切酶活性的聚合酶抑制扩增 Download PDF

Info

Publication number
CN101778952B
CN101778952B CN200880102467.XA CN200880102467A CN101778952B CN 101778952 B CN101778952 B CN 101778952B CN 200880102467 A CN200880102467 A CN 200880102467A CN 101778952 B CN101778952 B CN 101778952B
Authority
CN
China
Prior art keywords
sequence
blocking agent
target
oligonucleotides
agent oligonucleotides
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN200880102467.XA
Other languages
English (en)
Chinese (zh)
Other versions
CN101778952A (zh
Inventor
N·牛顿
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Original Assignee
F Hoffmann La Roche AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by F Hoffmann La Roche AG filed Critical F Hoffmann La Roche AG
Publication of CN101778952A publication Critical patent/CN101778952A/zh
Application granted granted Critical
Publication of CN101778952B publication Critical patent/CN101778952B/zh
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CN200880102467.XA 2007-08-08 2008-08-07 使用寡核苷酸和显著缺乏5’-3’核酸外切酶活性的聚合酶抑制扩增 Expired - Fee Related CN101778952B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US96408907P 2007-08-08 2007-08-08
US60/964089 2007-08-08
PCT/EP2008/006476 WO2009019008A1 (en) 2007-08-08 2008-08-07 Suppression of amplification using an oligonucleotide and a polymerase significantly lacking 5'-3' exonuclease activity

Publications (2)

Publication Number Publication Date
CN101778952A CN101778952A (zh) 2010-07-14
CN101778952B true CN101778952B (zh) 2016-05-25

Family

ID=39942715

Family Applications (1)

Application Number Title Priority Date Filing Date
CN200880102467.XA Expired - Fee Related CN101778952B (zh) 2007-08-08 2008-08-07 使用寡核苷酸和显著缺乏5’-3’核酸外切酶活性的聚合酶抑制扩增

Country Status (7)

Country Link
US (1) US8071338B2 (enExample)
EP (1) EP2173910B1 (enExample)
JP (1) JP5524838B2 (enExample)
CN (1) CN101778952B (enExample)
CA (1) CA2693384C (enExample)
ES (1) ES2562789T3 (enExample)
WO (1) WO2009019008A1 (enExample)

Families Citing this family (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2183379B1 (en) 2007-08-01 2015-04-15 Dana Farber Cancer Institute Enrichment of a target sequence
US8206929B2 (en) * 2009-01-07 2012-06-26 Roche Molecular Systems, Inc. Nucleic acid amplification with allele-specific suppression of sequence variants
CA2778532A1 (en) 2009-10-27 2011-05-12 Swift Biosciences, Inc. Polynucleotide primers and probes
ES2665500T3 (es) * 2010-03-08 2018-04-26 Dana-Farber Cancer Institute, Inc. Enriquecimiento de una PCR por COLD completa con secuencia de bloqueo de referencia
US9944979B2 (en) * 2010-09-10 2018-04-17 Lsi Medience Corporation Inhibition method of nucleic acid amplification by photoirradiation and method of selective nucleic acid amplification with high sensitivity
DK2633071T3 (en) 2010-10-27 2017-01-30 Harvard College COMPOSITIONS OF "MAINTENANCE" PRIMER DUPLEXES AND METHODS OF USE
KR20140010093A (ko) * 2011-02-28 2014-01-23 트랜스제노믹, 인크. 혼합 집단 중 표적 dna의 서열분석을 위한 키트 및 방법
US11130992B2 (en) 2011-03-31 2021-09-28 Dana-Farber Cancer Institute, Inc. Methods and compositions to enable multiplex COLD-PCR
WO2012151328A2 (en) 2011-05-02 2012-11-08 President And Fellows Of Harvard College Spatial sequestration of dynamic nucleic acid circuits
JP6404118B2 (ja) 2011-05-04 2018-10-10 バイオセプト インコーポレイティッド 核酸配列変種を検出するための方法
WO2012162621A1 (en) * 2011-05-26 2012-11-29 Brandeis University Methods for suppression pcr
EP2758458A4 (en) 2011-07-10 2015-10-21 Harvard College COMPOSITIONS AND METHODS FOR SELF-ASSEMBLING POLYMERS WITH COMPLEMENTARY MACROSCOPIC AND MICROSCOPIC SCALE UNITS
EP2570487A1 (en) 2011-09-16 2013-03-20 Lexogen GmbH Nucleic acid transcription method
CA2848240C (en) 2011-09-16 2020-09-29 Lexogen Gmbh Nucleic acid transcription method
US9260714B2 (en) 2011-12-02 2016-02-16 Roche Molecular Systems, Inc. Suppression of non-specific amplification with high-homology oligonucleotides
US9133490B2 (en) 2012-05-16 2015-09-15 Transgenomic, Inc. Step-up method for COLD-PCR enrichment
JP6652693B2 (ja) * 2013-04-29 2020-02-26 キアゲン ゲーエムベーハー ブロッキングオリゴヌクレオチドを用いたｄｎａ増幅の方法
EP3058099A4 (en) * 2013-10-19 2017-06-28 TrovaGene, Inc. Detecting mutations in disease over time
KR102343605B1 (ko) * 2014-05-19 2021-12-27 윌리엄 마쉬 라이스 유니버시티 비대립 유전자-특이적 프라이머 및 대립 유전자-특이적 블로커 올리고뉴클레오티드를 중첩하는 조성물을 사용한 대립 유전자-특이적 증폭
CA2957396C (en) * 2014-08-07 2021-03-09 Evrykleia LIANIDOU Method of determining pik3ca mutational status in a sample
US12391985B2 (en) 2014-08-07 2025-08-19 Pharmassist Ltd Method of determining PIK3CA mutational status in a sample
JP6766037B2 (ja) * 2014-09-26 2020-10-07 ヤンセン ファーマシューティカ エヌ．ベー． Ｆｇｆｒ阻害剤による治療に応答性であるがん患者を同定する際の、ｆｇｆｒ変異遺伝子パネルの使用
US9909169B2 (en) * 2014-12-17 2018-03-06 Roche Molecular Systems, Inc. Allele-specific amplification of nucleic acids using blocking oligonucleotides for wild type suppression
JP5813263B1 (ja) * 2015-03-31 2015-11-17 日鉄住金環境株式会社 遺伝子変異の検出方法及びそれに用いる蛍光標識オリゴヌクレオチド
US9657353B2 (en) 2015-06-26 2017-05-23 Great Basin Scientific, Inc. Specific detection of organisms derived from a sample
WO2017193025A1 (en) 2016-05-06 2017-11-09 William Marsh Rice University Stoichiometric nucleic acid purification using randomer capture probe libraries
WO2017214202A1 (en) * 2016-06-07 2017-12-14 Cepheid Thermostable polymerase inhibitor compositions and methods
WO2018111835A1 (en) 2016-12-12 2018-06-21 Dana-Farber Cancer Institute, Inc. Compositions and methods for molecular barcoding of dna molecules prior to mutation enrichment and/or mutation detection
WO2019023243A1 (en) 2017-07-24 2019-01-31 Dana-Farber Cancer Institute, Inc. METHODS AND COMPOSITIONS FOR SELECTING AND AMPLIFYING DNA TARGETS IN A SINGLE REACTION MIXTURE
WO2019099420A1 (en) * 2017-11-15 2019-05-23 Yan Wang A method for detecting multiple dna mutations and copy number variations
US12331350B2 (en) 2018-02-20 2025-06-17 William Marsh Rice University Systems and methods for allele enrichment using multiplexed blocker displacement amplification
WO2020016590A1 (en) 2018-07-19 2020-01-23 Biofidelity Ltd Improved polynucleotide sequence detection method
WO2020126595A1 (en) * 2018-12-17 2020-06-25 Illumina Cambridge Limited Primer oligonucleotide for sequencing
GB201919186D0 (en) 2019-12-23 2020-02-05 Biofidelity Ltd Simplified polynucleotide sequence detection method
WO2023039228A2 (en) * 2021-09-13 2023-03-16 Nexgen Cancer Detection Llc Methods for detecting a target polynucleotide in a sample comprising a mixture of the target polynucleotide and a non-target polynucleotide

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5849497A (en) * 1997-04-03 1998-12-15 The Research Foundation Of State University Of New York Specific inhibition of the polymerase chain reaction using a non-extendable oligonucleotide blocker
WO1999061661A1 (fr) * 1998-05-27 1999-12-02 Bio Merieux Procede d'amplification d'au moins une sequence nucleotidique particuliere et amorces de mise en oeuvre
WO2002086155A2 (en) * 2001-04-24 2002-10-31 Lion Bioscience Ag Method for the preferential nucleic acid amplification of selected nucleic acid regions

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2163393C (en) 1994-11-30 2003-04-22 Colleen Marie Nycz Amplification and detection of mycobacteria nucleic acids
US5801155A (en) * 1995-04-03 1998-09-01 Epoch Pharmaceuticals, Inc. Covalently linked oligonucleotide minor grove binder conjugates
EP1141384A2 (en) * 1999-01-06 2001-10-10 Cornell Research Foundation, Inc. Method for accelerating identification of single nucleotide polymorphisms and alignment of clones in genomic sequencing
GB0406863D0 (en) 2004-03-26 2004-04-28 Qiagen As Nucleic acid sequencing
DE602005022740D1 (de) 2004-09-10 2010-09-16 Human Genetic Signatures Pty Amplifikationsblocker umfassend interkalierende nukleinsäuren (ina) enthaltend interkalierende pseudonukleotide (ipn)

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5849497A (en) * 1997-04-03 1998-12-15 The Research Foundation Of State University Of New York Specific inhibition of the polymerase chain reaction using a non-extendable oligonucleotide blocker
WO1999061661A1 (fr) * 1998-05-27 1999-12-02 Bio Merieux Procede d'amplification d'au moins une sequence nucleotidique particuliere et amorces de mise en oeuvre
WO2002086155A2 (en) * 2001-04-24 2002-10-31 Lion Bioscience Ag Method for the preferential nucleic acid amplification of selected nucleic acid regions

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
Luo JD et al..Detection of rare mutant k-ras DNA in a single-tube reaction using peptide nucleic acid as both PCR clamp and sensor probe..《Nucleic Acids Research》.2006,第34卷(第2期), *
Patrick L Dominguez and Michael S Kolodney.Wild-type blocking polymerase chain reaction for detection of single nucleotide minority mutations from clinical specimens.《Oncogene》.2005,6830-6834. *
Seyama T et al..A novel blocker-PCR method for detection of rare mutant alleles in the presence of an excess amount of normal DNA..《Nucleic Acids Research》.1992,第20卷(第10期),2493-2496. *

Also Published As

Publication number Publication date
CA2693384C (en) 2015-04-14
HK1146094A1 (zh) 2011-05-13
JP5524838B2 (ja) 2014-06-18
EP2173910A1 (en) 2010-04-14
EP2173910B1 (en) 2015-12-09
CN101778952A (zh) 2010-07-14
US20090053720A1 (en) 2009-02-26
WO2009019008A1 (en) 2009-02-12
CA2693384A1 (en) 2009-02-12
ES2562789T3 (es) 2016-03-08
JP2010535480A (ja) 2010-11-25
US8071338B2 (en) 2011-12-06

Similar Documents

Publication Publication Date Title
CN101778952B (zh) 使用寡核苷酸和显著缺乏5’-3’核酸外切酶活性的聚合酶抑制扩增
CN107254540B (zh) 检测核酸差异的探针形式
ES2530273T3 (es) Amplificación de ácidos nucleicos específica de alelo
CN102119226B (zh) 校对引物延伸
KR102295290B1 (ko) Dna 증폭 기술
US20160369330A1 (en) Compositions for improved allele-specific pcr
WO2013093432A1 (en) Method for enriching and detection of variant target nucleic acids
JPWO2003102178A1 (ja) 遺伝子多型のタイピング方法
HK1146094B (en) Suppression of amplification using an oligonucleotide and a polymerase significantly lacking 5'-3' exonuclease activity
EP2347012A1 (en) Detection of target variants using a fluorescent labeland and a soluble quencher
WO2024141476A1 (en) Digital pcr assay designs for multiple hepatitis b virus gene targets and non-extendable blocker oligonucleotides therefor
HK1159192B (en) Proofreading primer extension

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
REG Reference to a national code

Ref country code: HK

Ref legal event code: DE

Ref document number: 1146094

Country of ref document: HK

C14 Grant of patent or utility model
GR01 Patent grant
REG Reference to a national code

Ref country code: HK

Ref legal event code: GR

Ref document number: 1146094

Country of ref document: HK

CF01 Termination of patent right due to non-payment of annual fee
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20160525

Termination date: 20190807