CN101778952B - 使用寡核苷酸和显著缺乏5’-3’核酸外切酶活性的聚合酶抑制扩增 - Google Patents

使用寡核苷酸和显著缺乏5’-3’核酸外切酶活性的聚合酶抑制扩增 Download PDF

Info

Publication number
CN101778952B
CN101778952B CN200880102467.XA CN200880102467A CN101778952B CN 101778952 B CN101778952 B CN 101778952B CN 200880102467 A CN200880102467 A CN 200880102467A CN 101778952 B CN101778952 B CN 101778952B
Authority
CN
China
Prior art keywords
sequence
blocking agent
target
oligonucleotides
agent oligonucleotides
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN200880102467.XA
Other languages
English (en)
Chinese (zh)
Other versions
CN101778952A (zh
Inventor
N·牛顿
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Original Assignee
F Hoffmann La Roche AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by F Hoffmann La Roche AG filed Critical F Hoffmann La Roche AG
Publication of CN101778952A publication Critical patent/CN101778952A/zh
Application granted granted Critical
Publication of CN101778952B publication Critical patent/CN101778952B/zh
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CN200880102467.XA 2007-08-08 2008-08-07 使用寡核苷酸和显著缺乏5’-3’核酸外切酶活性的聚合酶抑制扩增 Expired - Fee Related CN101778952B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US96408907P 2007-08-08 2007-08-08
US60/964089 2007-08-08
PCT/EP2008/006476 WO2009019008A1 (en) 2007-08-08 2008-08-07 Suppression of amplification using an oligonucleotide and a polymerase significantly lacking 5'-3' exonuclease activity

Publications (2)

Publication Number Publication Date
CN101778952A CN101778952A (zh) 2010-07-14
CN101778952B true CN101778952B (zh) 2016-05-25

Family

ID=39942715

Family Applications (1)

Application Number Title Priority Date Filing Date
CN200880102467.XA Expired - Fee Related CN101778952B (zh) 2007-08-08 2008-08-07 使用寡核苷酸和显著缺乏5’-3’核酸外切酶活性的聚合酶抑制扩增

Country Status (7)

Country Link
US (1) US8071338B2 (enExample)
EP (1) EP2173910B1 (enExample)
JP (1) JP5524838B2 (enExample)
CN (1) CN101778952B (enExample)
CA (1) CA2693384C (enExample)
ES (1) ES2562789T3 (enExample)
WO (1) WO2009019008A1 (enExample)

Families Citing this family (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2547053T3 (es) 2007-08-01 2015-10-01 Dana Farber Cancer Institute Enriquecimiento de una secuencia diana
US8206929B2 (en) * 2009-01-07 2012-06-26 Roche Molecular Systems, Inc. Nucleic acid amplification with allele-specific suppression of sequence variants
JP6147502B2 (ja) 2009-10-27 2017-06-14 スウィフト バイオサイエンシーズ， インコーポレイテッド ポリヌクレオチドプライマー及びプローブ
AU2011224534B2 (en) * 2010-03-08 2014-10-09 Dana-Farber Cancer Institute, Inc. Full COLD-PCR enrichment with reference blocking sequence
EP2615171B1 (en) * 2010-09-10 2015-11-04 LSI Medience Corporation Method for inhibiting nucleic acid amplification using light and highly sensitive method for selective nucleic acid amplification
EP3144396B1 (en) 2010-10-27 2020-01-01 President and Fellows of Harvard College Methods of use of toehold hairpin primer
JP2014507950A (ja) * 2011-02-28 2014-04-03 トランスゲノミック，インク． 混合個体群における標的ｄｎａを配列決定するためのキットおよび方法
EP2691541B1 (en) * 2011-03-31 2017-10-18 Dana-Farber Cancer Institute, Inc. Method for enriching in single-stranded mutant sequences from mixture of wild-type and mutant sequences
WO2012151328A2 (en) 2011-05-02 2012-11-08 President And Fellows Of Harvard College Spatial sequestration of dynamic nucleic acid circuits
KR102010601B1 (ko) * 2011-05-04 2019-08-13 바이오셉트 인코포레이티드 핵산 서열 변이체를 검출하는 방법
US9518292B2 (en) 2011-05-26 2016-12-13 Brandeis University Methods for suppression PCR
US9617392B2 (en) 2011-07-10 2017-04-11 President And Fellows Of Harvard College Compositions and methods for self-assembly of polymers with complementary macroscopic and microscopic scale units
LT2756098T (lt) 2011-09-16 2018-09-10 Lexogen Gmbh Nukleorūgščių molekulių bibliotekos sudarymo būdas
EP2570487A1 (en) 2011-09-16 2013-03-20 Lexogen GmbH Nucleic acid transcription method
US9260714B2 (en) 2011-12-02 2016-02-16 Roche Molecular Systems, Inc. Suppression of non-specific amplification with high-homology oligonucleotides
US9133490B2 (en) 2012-05-16 2015-09-15 Transgenomic, Inc. Step-up method for COLD-PCR enrichment
WO2014177540A1 (en) * 2013-04-29 2014-11-06 Qiagen Marseille A method for dna amplification with a blocking oligonucleotide
JP2016536013A (ja) * 2013-10-19 2016-11-24 トローバジーン インコーポレイテッド 疾患における変異の経時的な検出
KR102343605B1 (ko) * 2014-05-19 2021-12-27 윌리엄 마쉬 라이스 유니버시티 비대립 유전자-특이적 프라이머 및 대립 유전자-특이적 블로커 올리고뉴클레오티드를 중첩하는 조성물을 사용한 대립 유전자-특이적 증폭
EP3198026B1 (en) 2014-08-07 2019-10-09 Pharmassist Ltd Method of determining pik3ca mutational status in a sample
US12391985B2 (en) 2014-08-07 2025-08-19 Pharmassist Ltd Method of determining PIK3CA mutational status in a sample
CN107002141B (zh) * 2014-09-26 2021-11-02 詹森药业有限公司 使用fgfr突变基因组鉴定将对用fgfr抑制剂进行治疗有反应的癌症患者
US9909169B2 (en) * 2014-12-17 2018-03-06 Roche Molecular Systems, Inc. Allele-specific amplification of nucleic acids using blocking oligonucleotides for wild type suppression
JP5813263B1 (ja) * 2015-03-31 2015-11-17 日鉄住金環境株式会社 遺伝子変異の検出方法及びそれに用いる蛍光標識オリゴヌクレオチド
US9657353B2 (en) 2015-06-26 2017-05-23 Great Basin Scientific, Inc. Specific detection of organisms derived from a sample
US11414686B2 (en) 2016-05-06 2022-08-16 William Marsh Rice University Stoichiometric nucleic acid purification using randomer capture probe libraries
WO2017214202A1 (en) * 2016-06-07 2017-12-14 Cepheid Thermostable polymerase inhibitor compositions and methods
CA3046953A1 (en) 2016-12-12 2018-06-21 Dana Farber Cancer Institute, Inc. Compositions and methods for molecular barcoding of dna molecules prior to mutation enrichment and/or mutation detection
US11174511B2 (en) 2017-07-24 2021-11-16 Dana-Farber Cancer Institute, Inc. Methods and compositions for selecting and amplifying DNA targets in a single reaction mixture
WO2019099420A1 (en) * 2017-11-15 2019-05-23 Yan Wang A method for detecting multiple dna mutations and copy number variations
US12331350B2 (en) 2018-02-20 2025-06-17 William Marsh Rice University Systems and methods for allele enrichment using multiplexed blocker displacement amplification
JP6947464B2 (ja) 2018-07-19 2021-10-13 バイオフィデリティ・リミテッド 改善されたポリヌクレオチド配列検出法
CA3103750A1 (en) * 2018-12-17 2020-06-25 Illumina Cambridge Limited Primer oligonucleotide for sequencing
GB201919186D0 (en) 2019-12-23 2020-02-05 Biofidelity Ltd Simplified polynucleotide sequence detection method
JP2024531713A (ja) * 2021-09-13 2024-08-29 ネクスジェン キャンサー ディテクション エルエルシー 試料中の標的ポリヌクレオチドを検出するための方法

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5849497A (en) * 1997-04-03 1998-12-15 The Research Foundation Of State University Of New York Specific inhibition of the polymerase chain reaction using a non-extendable oligonucleotide blocker
WO1999061661A1 (fr) * 1998-05-27 1999-12-02 Bio Merieux Procede d'amplification d'au moins une sequence nucleotidique particuliere et amorces de mise en oeuvre
WO2002086155A2 (en) * 2001-04-24 2002-10-31 Lion Bioscience Ag Method for the preferential nucleic acid amplification of selected nucleic acid regions

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2163393C (en) 1994-11-30 2003-04-22 Colleen Marie Nycz Amplification and detection of mycobacteria nucleic acids
US5801155A (en) * 1995-04-03 1998-09-01 Epoch Pharmaceuticals, Inc. Covalently linked oligonucleotide minor grove binder conjugates
AU780119B2 (en) * 1999-01-06 2005-03-03 Cornell Research Foundation Inc. Accelerating identification of single nucleotide polymorphisms and alignment of clones in genomic sequencing
GB0406863D0 (en) 2004-03-26 2004-04-28 Qiagen As Nucleic acid sequencing
US7803580B2 (en) 2004-09-10 2010-09-28 Human Genetic Signatures Pty. Ltd. Amplification blocker comprising intercalating nucleic acids (INA) containing intercalating pseudonucleotides (IPN)

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5849497A (en) * 1997-04-03 1998-12-15 The Research Foundation Of State University Of New York Specific inhibition of the polymerase chain reaction using a non-extendable oligonucleotide blocker
WO1999061661A1 (fr) * 1998-05-27 1999-12-02 Bio Merieux Procede d'amplification d'au moins une sequence nucleotidique particuliere et amorces de mise en oeuvre
WO2002086155A2 (en) * 2001-04-24 2002-10-31 Lion Bioscience Ag Method for the preferential nucleic acid amplification of selected nucleic acid regions

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
Luo JD et al..Detection of rare mutant k-ras DNA in a single-tube reaction using peptide nucleic acid as both PCR clamp and sensor probe..《Nucleic Acids Research》.2006,第34卷(第2期), *
Patrick L Dominguez and Michael S Kolodney.Wild-type blocking polymerase chain reaction for detection of single nucleotide minority mutations from clinical specimens.《Oncogene》.2005,6830-6834. *
Seyama T et al..A novel blocker-PCR method for detection of rare mutant alleles in the presence of an excess amount of normal DNA..《Nucleic Acids Research》.1992,第20卷(第10期),2493-2496. *

Also Published As

Publication number Publication date
EP2173910B1 (en) 2015-12-09
US8071338B2 (en) 2011-12-06
JP5524838B2 (ja) 2014-06-18
CA2693384C (en) 2015-04-14
ES2562789T3 (es) 2016-03-08
JP2010535480A (ja) 2010-11-25
EP2173910A1 (en) 2010-04-14
CA2693384A1 (en) 2009-02-12
CN101778952A (zh) 2010-07-14
HK1146094A1 (zh) 2011-05-13
US20090053720A1 (en) 2009-02-26
WO2009019008A1 (en) 2009-02-12

Similar Documents

Publication Publication Date Title
CN101778952B (zh) 使用寡核苷酸和显著缺乏5’-3’核酸外切酶活性的聚合酶抑制扩增
CN107254540B (zh) 检测核酸差异的探针形式
ES2530273T3 (es) Amplificación de ácidos nucleicos específica de alelo
CN102119226B (zh) 校对引物延伸
KR102295290B1 (ko) Dna 증폭 기술
US20160369330A1 (en) Compositions for improved allele-specific pcr
JPWO2003102178A1 (ja) 遺伝子多型のタイピング方法
HK1146094B (en) Suppression of amplification using an oligonucleotide and a polymerase significantly lacking 5'-3' exonuclease activity
WO2024141476A1 (en) Digital pcr assay designs for multiple hepatitis b virus gene targets and non-extendable blocker oligonucleotides therefor
HK1159192B (en) Proofreading primer extension

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
REG Reference to a national code

Ref country code: HK

Ref legal event code: DE

Ref document number: 1146094

Country of ref document: HK

C14 Grant of patent or utility model
GR01 Patent grant
REG Reference to a national code

Ref country code: HK

Ref legal event code: GR

Ref document number: 1146094

Country of ref document: HK

CF01 Termination of patent right due to non-payment of annual fee
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20160525

Termination date: 20190807