CN101778952B - 使用寡核苷酸和显著缺乏5’-3’核酸外切酶活性的聚合酶抑制扩增 - Google Patents

使用寡核苷酸和显著缺乏5’-3’核酸外切酶活性的聚合酶抑制扩增 Download PDF

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Publication number
CN101778952B
CN101778952B CN200880102467.XA CN200880102467A CN101778952B CN 101778952 B CN101778952 B CN 101778952B CN 200880102467 A CN200880102467 A CN 200880102467A CN 101778952 B CN101778952 B CN 101778952B
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sequence
blocking agent
target
oligonucleotides
agent oligonucleotides
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CN101778952A (zh
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N·牛顿
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F Hoffmann La Roche AG
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F Hoffmann La Roche AG
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CN200880102467.XA 2007-08-08 2008-08-07 使用寡核苷酸和显著缺乏5’-3’核酸外切酶活性的聚合酶抑制扩增 Expired - Fee Related CN101778952B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US96408907P 2007-08-08 2007-08-08
US60/964089 2007-08-08
PCT/EP2008/006476 WO2009019008A1 (en) 2007-08-08 2008-08-07 Suppression of amplification using an oligonucleotide and a polymerase significantly lacking 5'-3' exonuclease activity

Publications (2)

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CN101778952A CN101778952A (zh) 2010-07-14
CN101778952B true CN101778952B (zh) 2016-05-25

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CN200880102467.XA Expired - Fee Related CN101778952B (zh) 2007-08-08 2008-08-07 使用寡核苷酸和显著缺乏5’-3’核酸外切酶活性的聚合酶抑制扩增

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US (1) US8071338B2 (enExample)
EP (1) EP2173910B1 (enExample)
JP (1) JP5524838B2 (enExample)
CN (1) CN101778952B (enExample)
CA (1) CA2693384C (enExample)
ES (1) ES2562789T3 (enExample)
WO (1) WO2009019008A1 (enExample)

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KR101550489B1 (ko) 2010-03-08 2015-09-07 다나-파버 캔서 인스티튜트 인크. 기준 차단 서열을 이용한 완전 ｃｏｌｄ－ｐｃｒ 풍부화
JP5795317B2 (ja) * 2010-09-10 2015-10-14 株式会社Ｌｓｉメディエンス 光を用いた核酸の増幅抑制方法および高感度な選択的核酸増幅方法
CN103328654B (zh) 2010-10-27 2017-07-11 哈佛学院院长等 立足点引物双链体的组合物及其使用方法
CN103517993A (zh) * 2011-02-28 2014-01-15 环球基因有限公司 用于对混合群体中的靶dna进行测序的试剂盒和方法
EP3333269B1 (en) * 2011-03-31 2021-05-05 Dana-Farber Cancer Institute, Inc. Methods to enable multiplex cold-pcr
US9796748B2 (en) 2011-05-02 2017-10-24 President And Fellows Of Harvard College Spatial sequestration of dynamic nucleic acid circuits
KR20210056458A (ko) 2011-05-04 2021-05-18 바이오셉트 인코포레이티드 핵산 서열 변이체를 검출하는 방법
US9518292B2 (en) 2011-05-26 2016-12-13 Brandeis University Methods for suppression PCR
EP2758458A4 (en) 2011-07-10 2015-10-21 Harvard College COMPOSITIONS AND METHODS FOR SELF-ASSEMBLING POLYMERS WITH COMPLEMENTARY MACROSCOPIC AND MICROSCOPIC SCALE UNITS
WO2013038010A2 (en) 2011-09-16 2013-03-21 Lexogen Gmbh Nucleic acid transcription method
EP2570487A1 (en) 2011-09-16 2013-03-20 Lexogen GmbH Nucleic acid transcription method
US9260714B2 (en) * 2011-12-02 2016-02-16 Roche Molecular Systems, Inc. Suppression of non-specific amplification with high-homology oligonucleotides
US9133490B2 (en) 2012-05-16 2015-09-15 Transgenomic, Inc. Step-up method for COLD-PCR enrichment
EP2992110B1 (en) * 2013-04-29 2017-10-25 QIAGEN Marseille S.A.S. A method for dna amplification with a blocking oligonucleotide
WO2015058176A1 (en) * 2013-10-19 2015-04-23 Trovagene, Inc. Detecting mutations in disease over time
CN114032234A (zh) * 2014-05-19 2022-02-11 威廉马歇莱思大学 使用重叠非等位基因特异性引物和等位基因特异性阻断剂寡核苷酸进行等位基因特异性扩增
US12391985B2 (en) 2014-08-07 2025-08-19 Pharmassist Ltd Method of determining PIK3CA mutational status in a sample
SI3198026T1 (sl) * 2014-08-07 2020-02-28 Pharmassist Ltd Metoda za analiziranje DNA PIK3CA mutantnega statusa v vzorcu
SG11201702381QA (en) 2014-09-26 2017-04-27 Janssen Pharmaceutica Nv Use of fgfr mutant gene panels in identifying cancer patients that will be responsive to treatment with an fgfr inhibitor
US9909169B2 (en) * 2014-12-17 2018-03-06 Roche Molecular Systems, Inc. Allele-specific amplification of nucleic acids using blocking oligonucleotides for wild type suppression
JP5813263B1 (ja) * 2015-03-31 2015-11-17 日鉄住金環境株式会社 遺伝子変異の検出方法及びそれに用いる蛍光標識オリゴヌクレオチド
US9657353B2 (en) 2015-06-26 2017-05-23 Great Basin Scientific, Inc. Specific detection of organisms derived from a sample
WO2017193025A1 (en) 2016-05-06 2017-11-09 William Marsh Rice University Stoichiometric nucleic acid purification using randomer capture probe libraries
EP3464596B1 (en) * 2016-06-07 2025-07-30 Cepheid Thermostable polymerase inhibitor compositions and methods
WO2018111835A1 (en) 2016-12-12 2018-06-21 Dana-Farber Cancer Institute, Inc. Compositions and methods for molecular barcoding of dna molecules prior to mutation enrichment and/or mutation detection
US11174511B2 (en) 2017-07-24 2021-11-16 Dana-Farber Cancer Institute, Inc. Methods and compositions for selecting and amplifying DNA targets in a single reaction mixture
WO2019099420A1 (en) * 2017-11-15 2019-05-23 Yan Wang A method for detecting multiple dna mutations and copy number variations
US12331350B2 (en) 2018-02-20 2025-06-17 William Marsh Rice University Systems and methods for allele enrichment using multiplexed blocker displacement amplification
IL280222B2 (en) 2018-07-19 2025-08-01 Biofidelity Ltd Improved polynucleotide sequence detection method
EP3899036A1 (en) * 2018-12-17 2021-10-27 Illumina Cambridge Limited Primer oligonucleotide for sequencing
GB201919186D0 (en) 2019-12-23 2020-02-05 Biofidelity Ltd Simplified polynucleotide sequence detection method
EP4402283A4 (en) * 2021-09-13 2025-11-12 Nexgen Cancer Detection Llc METHODS FOR DETECTING A TARGET POLYNUCLEOTIDE IN A SAMPLE

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US5849497A (en) * 1997-04-03 1998-12-15 The Research Foundation Of State University Of New York Specific inhibition of the polymerase chain reaction using a non-extendable oligonucleotide blocker
WO1999061661A1 (fr) * 1998-05-27 1999-12-02 Bio Merieux Procede d'amplification d'au moins une sequence nucleotidique particuliere et amorces de mise en oeuvre
WO2002086155A2 (en) * 2001-04-24 2002-10-31 Lion Bioscience Ag Method for the preferential nucleic acid amplification of selected nucleic acid regions

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US5801155A (en) * 1995-04-03 1998-09-01 Epoch Pharmaceuticals, Inc. Covalently linked oligonucleotide minor grove binder conjugates
CA2356697C (en) * 1999-01-06 2010-06-22 Cornell Research Foundation, Inc. Accelerating identification of single nucleotide polymorphisms and alignment of clones in genomic sequencing
GB0406863D0 (en) 2004-03-26 2004-04-28 Qiagen As Nucleic acid sequencing
WO2006026828A1 (en) 2004-09-10 2006-03-16 Human Genetic Signatures Pty Ltd Amplification blocker comprising intercalating nucleic acids (tna) containing intercalating pseudonucleotides (ipn)

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5849497A (en) * 1997-04-03 1998-12-15 The Research Foundation Of State University Of New York Specific inhibition of the polymerase chain reaction using a non-extendable oligonucleotide blocker
WO1999061661A1 (fr) * 1998-05-27 1999-12-02 Bio Merieux Procede d'amplification d'au moins une sequence nucleotidique particuliere et amorces de mise en oeuvre
WO2002086155A2 (en) * 2001-04-24 2002-10-31 Lion Bioscience Ag Method for the preferential nucleic acid amplification of selected nucleic acid regions

Non-Patent Citations (3)

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Title
Luo JD et al..Detection of rare mutant k-ras DNA in a single-tube reaction using peptide nucleic acid as both PCR clamp and sensor probe..《Nucleic Acids Research》.2006,第34卷(第2期), *
Patrick L Dominguez and Michael S Kolodney.Wild-type blocking polymerase chain reaction for detection of single nucleotide minority mutations from clinical specimens.《Oncogene》.2005,6830-6834. *
Seyama T et al..A novel blocker-PCR method for detection of rare mutant alleles in the presence of an excess amount of normal DNA..《Nucleic Acids Research》.1992,第20卷(第10期),2493-2496. *

Also Published As

Publication number Publication date
US8071338B2 (en) 2011-12-06
JP5524838B2 (ja) 2014-06-18
EP2173910A1 (en) 2010-04-14
CA2693384A1 (en) 2009-02-12
CA2693384C (en) 2015-04-14
ES2562789T3 (es) 2016-03-08
WO2009019008A1 (en) 2009-02-12
JP2010535480A (ja) 2010-11-25
CN101778952A (zh) 2010-07-14
HK1146094A1 (zh) 2011-05-13
US20090053720A1 (en) 2009-02-26
EP2173910B1 (en) 2015-12-09

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