JP5524838B2 - オリゴヌクレオチド、及び５’−３’エキソヌクレアーゼ活性を著しく欠如するポリメラーゼを使用する、増幅の抑制 - Google Patents

オリゴヌクレオチド、及び５’−３’エキソヌクレアーゼ活性を著しく欠如するポリメラーゼを使用する、増幅の抑制 Download PDF

Info

Publication number
JP5524838B2
JP5524838B2 JP2010519376A JP2010519376A JP5524838B2 JP 5524838 B2 JP5524838 B2 JP 5524838B2 JP 2010519376 A JP2010519376 A JP 2010519376A JP 2010519376 A JP2010519376 A JP 2010519376A JP 5524838 B2 JP5524838 B2 JP 5524838B2
Authority
JP
Japan
Prior art keywords
sequence
blocker oligonucleotide
target
target sequence
polymerase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
JP2010519376A
Other languages
English (en)
Japanese (ja)
Other versions
JP2010535480A (ja
JP2010535480A5 (enExample
Inventor
ニュートン，ニコラス
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Original Assignee
F Hoffmann La Roche AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by F Hoffmann La Roche AG filed Critical F Hoffmann La Roche AG
Publication of JP2010535480A publication Critical patent/JP2010535480A/ja
Publication of JP2010535480A5 publication Critical patent/JP2010535480A5/ja
Application granted granted Critical
Publication of JP5524838B2 publication Critical patent/JP5524838B2/ja
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
JP2010519376A 2007-08-08 2008-08-07 オリゴヌクレオチド、及び５’−３’エキソヌクレアーゼ活性を著しく欠如するポリメラーゼを使用する、増幅の抑制 Expired - Fee Related JP5524838B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US96408907P 2007-08-08 2007-08-08
US60/964,089 2007-08-08
PCT/EP2008/006476 WO2009019008A1 (en) 2007-08-08 2008-08-07 Suppression of amplification using an oligonucleotide and a polymerase significantly lacking 5'-3' exonuclease activity

Publications (3)

Publication Number Publication Date
JP2010535480A JP2010535480A (ja) 2010-11-25
JP2010535480A5 JP2010535480A5 (enExample) 2011-06-23
JP5524838B2 true JP5524838B2 (ja) 2014-06-18

Family

ID=39942715

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2010519376A Expired - Fee Related JP5524838B2 (ja) 2007-08-08 2008-08-07 オリゴヌクレオチド、及び５’−３’エキソヌクレアーゼ活性を著しく欠如するポリメラーゼを使用する、増幅の抑制

Country Status (7)

Country Link
US (1) US8071338B2 (enExample)
EP (1) EP2173910B1 (enExample)
JP (1) JP5524838B2 (enExample)
CN (1) CN101778952B (enExample)
CA (1) CA2693384C (enExample)
ES (1) ES2562789T3 (enExample)
WO (1) WO2009019008A1 (enExample)

Families Citing this family (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PT2183379E (pt) 2007-08-01 2015-09-25 Dana Farber Cancer Inst Inc Enriquecimento de uma sequência alvo
US8206929B2 (en) * 2009-01-07 2012-06-26 Roche Molecular Systems, Inc. Nucleic acid amplification with allele-specific suppression of sequence variants
US20110129832A1 (en) 2009-10-27 2011-06-02 Swift Biosciences, Inc. Polynucleotide Primers and Probes
AU2011224534B2 (en) 2010-03-08 2014-10-09 Dana-Farber Cancer Institute, Inc. Full COLD-PCR enrichment with reference blocking sequence
KR101851382B1 (ko) * 2010-09-10 2018-04-23 가부시키가이샤 엘에스아이 메디엔스 광을 이용한 핵산의 증폭 억제 방법 및 고감도의 선택적 핵산 증폭 방법
DK2633071T3 (en) 2010-10-27 2017-01-30 Harvard College COMPOSITIONS OF "MAINTENANCE" PRIMER DUPLEXES AND METHODS OF USE
US20120225421A1 (en) * 2011-02-28 2012-09-06 Transgenomic, Inc. Kit and method for sequencing a target dna in a mixed population
DK2691541T3 (en) * 2011-03-31 2018-01-22 Dana Farber Cancer Inst Inc PROCEDURE FOR ENRICHMENT OF SINGLE DRAWED MUTANTS SEQUENCES FROM A MIXTURE OF WILD TYPE AND MUTANTS
WO2012151328A2 (en) 2011-05-02 2012-11-08 President And Fellows Of Harvard College Spatial sequestration of dynamic nucleic acid circuits
KR102252447B1 (ko) 2011-05-04 2021-05-17 바이오셉트 인코포레이티드 핵산 서열 변이체를 검출하는 방법
US9518292B2 (en) 2011-05-26 2016-12-13 Brandeis University Methods for suppression PCR
US9617392B2 (en) 2011-07-10 2017-04-11 President And Fellows Of Harvard College Compositions and methods for self-assembly of polymers with complementary macroscopic and microscopic scale units
EP2570487A1 (en) 2011-09-16 2013-03-20 Lexogen GmbH Nucleic acid transcription method
EP3263718A1 (en) 2011-09-16 2018-01-03 Lexogen GmbH Nucleic acid transcription method
US9260714B2 (en) 2011-12-02 2016-02-16 Roche Molecular Systems, Inc. Suppression of non-specific amplification with high-homology oligonucleotides
US9133490B2 (en) 2012-05-16 2015-09-15 Transgenomic, Inc. Step-up method for COLD-PCR enrichment
WO2014177540A1 (en) * 2013-04-29 2014-11-06 Qiagen Marseille A method for dna amplification with a blocking oligonucleotide
CA2926722A1 (en) * 2013-10-19 2015-04-23 Trovagene, Inc. Detecting mutations in disease over time
EP4512908A3 (en) * 2014-05-19 2025-03-26 William Marsh Rice University Allele-specific amplification using a composition of overlapping non-allele-specific primer and allele-specific blocker oligonucleotides
PL3198026T3 (pl) 2014-08-07 2020-05-18 Pharmassist Ltd Metody określania mutacji genu pik3ca w próbce
US12391985B2 (en) 2014-08-07 2025-08-19 Pharmassist Ltd Method of determining PIK3CA mutational status in a sample
US20160090633A1 (en) 2014-09-26 2016-03-31 Janssen Pharmaceutica Nv Use of fgfr mutant gene panels in identifying cancer patients that will be responsive to treatment with an fgfr inhibitor
US9909169B2 (en) * 2014-12-17 2018-03-06 Roche Molecular Systems, Inc. Allele-specific amplification of nucleic acids using blocking oligonucleotides for wild type suppression
JP5813263B1 (ja) * 2015-03-31 2015-11-17 日鉄住金環境株式会社 遺伝子変異の検出方法及びそれに用いる蛍光標識オリゴヌクレオチド
US9657353B2 (en) 2015-06-26 2017-05-23 Great Basin Scientific, Inc. Specific detection of organisms derived from a sample
WO2017193025A1 (en) 2016-05-06 2017-11-09 William Marsh Rice University Stoichiometric nucleic acid purification using randomer capture probe libraries
EP3464596B1 (en) * 2016-06-07 2025-07-30 Cepheid Thermostable polymerase inhibitor compositions and methods
WO2018111835A1 (en) 2016-12-12 2018-06-21 Dana-Farber Cancer Institute, Inc. Compositions and methods for molecular barcoding of dna molecules prior to mutation enrichment and/or mutation detection
US11174511B2 (en) 2017-07-24 2021-11-16 Dana-Farber Cancer Institute, Inc. Methods and compositions for selecting and amplifying DNA targets in a single reaction mixture
WO2019099420A1 (en) * 2017-11-15 2019-05-23 Yan Wang A method for detecting multiple dna mutations and copy number variations
CN111683958A (zh) 2018-02-20 2020-09-18 威廉马歇莱思大学 使用多重抑制探针置换扩增进行等位基因富集的系统和方法
EP3889273A1 (en) 2018-07-19 2021-10-06 Biofidelity Ltd Improved polynucleotide sequence detection method
AU2019411267B2 (en) * 2018-12-17 2025-07-17 Illumina Cambridge Limited Primer oligonucleotide for sequencing
GB201919186D0 (en) 2019-12-23 2020-02-05 Biofidelity Ltd Simplified polynucleotide sequence detection method
WO2023039228A2 (en) * 2021-09-13 2023-03-16 Nexgen Cancer Detection Llc Methods for detecting a target polynucleotide in a sample comprising a mixture of the target polynucleotide and a non-target polynucleotide

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2163393C (en) 1994-11-30 2003-04-22 Colleen Marie Nycz Amplification and detection of mycobacteria nucleic acids
US5801155A (en) * 1995-04-03 1998-09-01 Epoch Pharmaceuticals, Inc. Covalently linked oligonucleotide minor grove binder conjugates
US5849497A (en) 1997-04-03 1998-12-15 The Research Foundation Of State University Of New York Specific inhibition of the polymerase chain reaction using a non-extendable oligonucleotide blocker
FR2779154B1 (fr) 1998-05-27 2002-07-12 Bio Merieux Procede d'amplification d'au moins une sequence nucleotidique particuliere et amorces de mise en oeuvre
CA2356697C (en) * 1999-01-06 2010-06-22 Cornell Research Foundation, Inc. Accelerating identification of single nucleotide polymorphisms and alignment of clones in genomic sequencing
EP1253205A1 (en) 2001-04-24 2002-10-30 LION Bioscience AG Method of blocking amplification of selected sequences
GB0406863D0 (en) 2004-03-26 2004-04-28 Qiagen As Nucleic acid sequencing
WO2006026828A1 (en) 2004-09-10 2006-03-16 Human Genetic Signatures Pty Ltd Amplification blocker comprising intercalating nucleic acids (tna) containing intercalating pseudonucleotides (ipn)

Also Published As

Publication number Publication date
HK1146094A1 (zh) 2011-05-13
EP2173910A1 (en) 2010-04-14
EP2173910B1 (en) 2015-12-09
JP2010535480A (ja) 2010-11-25
US20090053720A1 (en) 2009-02-26
CA2693384A1 (en) 2009-02-12
ES2562789T3 (es) 2016-03-08
CN101778952B (zh) 2016-05-25
CA2693384C (en) 2015-04-14
WO2009019008A1 (en) 2009-02-12
CN101778952A (zh) 2010-07-14
US8071338B2 (en) 2011-12-06

Similar Documents

Publication Publication Date Title
JP5524838B2 (ja) オリゴヌクレオチド、及び５’−３’エキソヌクレアーゼ活性を著しく欠如するポリメラーゼを使用する、増幅の抑制
US7989168B2 (en) Proofreading primer extension
CA2770241C (en) Method and probes to detect nucleic acid differences
ES2530273T3 (es) Amplificación de ácidos nucleicos específica de alelo
CN116064747A (zh) 用于变体检测的方法
US20160369330A1 (en) Compositions for improved allele-specific pcr
CN114686573B (zh) 一种检测靶核酸拷贝数重复的方法和试剂盒
US20160348158A1 (en) Format of Probes to Detect Nucleic Acid Differences
HK1146094B (en) Suppression of amplification using an oligonucleotide and a polymerase significantly lacking 5'-3' exonuclease activity
HK1159192B (en) Proofreading primer extension
JP2017163889A (ja) Ｋ−ｒａｓ遺伝子増幅用フォワードプライマーセット、Ｋ−ｒａｓ遺伝子増幅用キット、Ｋ−ｒａｓ遺伝子増幅方法、多型解析方法、及び薬効判定方法

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20110421

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20110421

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20130521

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20130821

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20131112

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20140212

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20140311

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20140410

R150 Certificate of patent or registration of utility model

Ref document number: 5524838

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

LAPS Cancellation because of no payment of annual fees