CN101778952B - 使用寡核苷酸和显著缺乏5’-3’核酸外切酶活性的聚合酶抑制扩增 - Google Patents

使用寡核苷酸和显著缺乏5’-3’核酸外切酶活性的聚合酶抑制扩增 Download PDF

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CN101778952B
CN101778952B CN200880102467.XA CN200880102467A CN101778952B CN 101778952 B CN101778952 B CN 101778952B CN 200880102467 A CN200880102467 A CN 200880102467A CN 101778952 B CN101778952 B CN 101778952B
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sequence
blocking agent
target
oligonucleotides
agent oligonucleotides
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CN101778952A (zh
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N·牛顿
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F Hoffmann La Roche AG
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F Hoffmann La Roche AG
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
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  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CN200880102467.XA 2007-08-08 2008-08-07 使用寡核苷酸和显著缺乏5’-3’核酸外切酶活性的聚合酶抑制扩增 Expired - Fee Related CN101778952B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US96408907P 2007-08-08 2007-08-08
US60/964089 2007-08-08
PCT/EP2008/006476 WO2009019008A1 (en) 2007-08-08 2008-08-07 Suppression of amplification using an oligonucleotide and a polymerase significantly lacking 5'-3' exonuclease activity

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CN101778952A CN101778952A (zh) 2010-07-14
CN101778952B true CN101778952B (zh) 2016-05-25

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CN200880102467.XA Expired - Fee Related CN101778952B (zh) 2007-08-08 2008-08-07 使用寡核苷酸和显著缺乏5’-3’核酸外切酶活性的聚合酶抑制扩增

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US (1) US8071338B2 (cg-RX-API-DMAC7.html)
EP (1) EP2173910B1 (cg-RX-API-DMAC7.html)
JP (1) JP5524838B2 (cg-RX-API-DMAC7.html)
CN (1) CN101778952B (cg-RX-API-DMAC7.html)
CA (1) CA2693384C (cg-RX-API-DMAC7.html)
ES (1) ES2562789T3 (cg-RX-API-DMAC7.html)
WO (1) WO2009019008A1 (cg-RX-API-DMAC7.html)

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WO2012151328A2 (en) 2011-05-02 2012-11-08 President And Fellows Of Harvard College Spatial sequestration of dynamic nucleic acid circuits
CN103635593B (zh) * 2011-05-04 2018-03-20 生物概念股份有限公司 用于检测核酸序列变体的方法
US9518292B2 (en) 2011-05-26 2016-12-13 Brandeis University Methods for suppression PCR
US9617392B2 (en) 2011-07-10 2017-04-11 President And Fellows Of Harvard College Compositions and methods for self-assembly of polymers with complementary macroscopic and microscopic scale units
KR102087062B1 (ko) 2011-09-16 2020-03-11 렉소겐 게엠베하 핵산 전사 방법
EP2570487A1 (en) 2011-09-16 2013-03-20 Lexogen GmbH Nucleic acid transcription method
US9260714B2 (en) * 2011-12-02 2016-02-16 Roche Molecular Systems, Inc. Suppression of non-specific amplification with high-homology oligonucleotides
US9133490B2 (en) 2012-05-16 2015-09-15 Transgenomic, Inc. Step-up method for COLD-PCR enrichment
EP2992110B1 (en) * 2013-04-29 2017-10-25 QIAGEN Marseille S.A.S. A method for dna amplification with a blocking oligonucleotide
CA2926722A1 (en) * 2013-10-19 2015-04-23 Trovagene, Inc. Detecting mutations in disease over time
ES2861353T3 (es) * 2014-05-19 2021-10-06 Univ Rice William M Amplificación específica de alelo utilizando una composición de oligonucleótidos cebadores no específicos de alelo y bloqueantes específicos de alelo solapantes
US12391985B2 (en) 2014-08-07 2025-08-19 Pharmassist Ltd Method of determining PIK3CA mutational status in a sample
PT3198026T (pt) 2014-08-07 2020-01-16 Pharmassist Ltd Método de determinação do estado mutacional de pik3ca numa amostra
HUE058219T2 (hu) 2014-09-26 2022-07-28 Janssen Pharmaceutica Nv FGFR-mutánsgénpanel alkalmazása olyan rákbetegek azonosításában, akik FGFR-inhibitorral történõ kezelésre reszponzívak lesznek
US9909169B2 (en) * 2014-12-17 2018-03-06 Roche Molecular Systems, Inc. Allele-specific amplification of nucleic acids using blocking oligonucleotides for wild type suppression
JP5813263B1 (ja) * 2015-03-31 2015-11-17 日鉄住金環境株式会社 遺伝子変異の検出方法及びそれに用いる蛍光標識オリゴヌクレオチド
US9657353B2 (en) 2015-06-26 2017-05-23 Great Basin Scientific, Inc. Specific detection of organisms derived from a sample
WO2017193025A1 (en) 2016-05-06 2017-11-09 William Marsh Rice University Stoichiometric nucleic acid purification using randomer capture probe libraries
JP7106461B2 (ja) * 2016-06-07 2022-07-26 セファイド 熱安定性ポリメラーゼ阻害剤組成物及び方法
WO2018111835A1 (en) 2016-12-12 2018-06-21 Dana-Farber Cancer Institute, Inc. Compositions and methods for molecular barcoding of dna molecules prior to mutation enrichment and/or mutation detection
WO2019023243A1 (en) 2017-07-24 2019-01-31 Dana-Farber Cancer Institute, Inc. METHODS AND COMPOSITIONS FOR SELECTING AND AMPLIFYING DNA TARGETS IN A SINGLE REACTION MIXTURE
WO2019099420A1 (en) * 2017-11-15 2019-05-23 Yan Wang A method for detecting multiple dna mutations and copy number variations
US12331350B2 (en) 2018-02-20 2025-06-17 William Marsh Rice University Systems and methods for allele enrichment using multiplexed blocker displacement amplification
KR102589502B1 (ko) 2018-07-19 2023-10-13 바이오피델리티 엘티디 개선된 폴리뉴클레오티드 서열 탐지 방법
EP3899036A1 (en) * 2018-12-17 2021-10-27 Illumina Cambridge Limited Primer oligonucleotide for sequencing
GB201919186D0 (en) 2019-12-23 2020-02-05 Biofidelity Ltd Simplified polynucleotide sequence detection method
JP2024531713A (ja) * 2021-09-13 2024-08-29 ネクスジェン キャンサー ディテクション エルエルシー 試料中の標的ポリヌクレオチドを検出するための方法

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WO1999061661A1 (fr) * 1998-05-27 1999-12-02 Bio Merieux Procede d'amplification d'au moins une sequence nucleotidique particuliere et amorces de mise en oeuvre
WO2002086155A2 (en) * 2001-04-24 2002-10-31 Lion Bioscience Ag Method for the preferential nucleic acid amplification of selected nucleic acid regions

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GB0406863D0 (en) 2004-03-26 2004-04-28 Qiagen As Nucleic acid sequencing
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Patent Citations (3)

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US5849497A (en) * 1997-04-03 1998-12-15 The Research Foundation Of State University Of New York Specific inhibition of the polymerase chain reaction using a non-extendable oligonucleotide blocker
WO1999061661A1 (fr) * 1998-05-27 1999-12-02 Bio Merieux Procede d'amplification d'au moins une sequence nucleotidique particuliere et amorces de mise en oeuvre
WO2002086155A2 (en) * 2001-04-24 2002-10-31 Lion Bioscience Ag Method for the preferential nucleic acid amplification of selected nucleic acid regions

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Publication number Publication date
EP2173910A1 (en) 2010-04-14
US8071338B2 (en) 2011-12-06
CA2693384C (en) 2015-04-14
CA2693384A1 (en) 2009-02-12
HK1146094A1 (zh) 2011-05-13
EP2173910B1 (en) 2015-12-09
JP2010535480A (ja) 2010-11-25
WO2009019008A1 (en) 2009-02-12
JP5524838B2 (ja) 2014-06-18
US20090053720A1 (en) 2009-02-26
CN101778952A (zh) 2010-07-14
ES2562789T3 (es) 2016-03-08

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