CA2693384C - Suppression of amplification using an oligonucleotide and a polymerase significantly lacking 5'-3' exonuclease activity - Google Patents

Suppression of amplification using an oligonucleotide and a polymerase significantly lacking 5'-3' exonuclease activity Download PDF

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Publication number
CA2693384C
CA2693384C CA2693384A CA2693384A CA2693384C CA 2693384 C CA2693384 C CA 2693384C CA 2693384 A CA2693384 A CA 2693384A CA 2693384 A CA2693384 A CA 2693384A CA 2693384 C CA2693384 C CA 2693384C
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CA
Canada
Prior art keywords
sequence
target
blocker oligonucleotide
nucleotide
oligonucleotide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CA2693384A
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English (en)
French (fr)
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CA2693384A1 (en
Inventor
Nicolas Newton
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F Hoffmann La Roche AG
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F Hoffmann La Roche AG
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Publication of CA2693384A1 publication Critical patent/CA2693384A1/en
Application granted granted Critical
Publication of CA2693384C publication Critical patent/CA2693384C/en
Expired - Fee Related legal-status Critical Current
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CA2693384A 2007-08-08 2008-08-07 Suppression of amplification using an oligonucleotide and a polymerase significantly lacking 5'-3' exonuclease activity Expired - Fee Related CA2693384C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US96408907P 2007-08-08 2007-08-08
US60/964,089 2007-08-08
PCT/EP2008/006476 WO2009019008A1 (en) 2007-08-08 2008-08-07 Suppression of amplification using an oligonucleotide and a polymerase significantly lacking 5'-3' exonuclease activity

Publications (2)

Publication Number Publication Date
CA2693384A1 CA2693384A1 (en) 2009-02-12
CA2693384C true CA2693384C (en) 2015-04-14

Family

ID=39942715

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2693384A Expired - Fee Related CA2693384C (en) 2007-08-08 2008-08-07 Suppression of amplification using an oligonucleotide and a polymerase significantly lacking 5'-3' exonuclease activity

Country Status (7)

Country Link
US (1) US8071338B2 (cg-RX-API-DMAC7.html)
EP (1) EP2173910B1 (cg-RX-API-DMAC7.html)
JP (1) JP5524838B2 (cg-RX-API-DMAC7.html)
CN (1) CN101778952B (cg-RX-API-DMAC7.html)
CA (1) CA2693384C (cg-RX-API-DMAC7.html)
ES (1) ES2562789T3 (cg-RX-API-DMAC7.html)
WO (1) WO2009019008A1 (cg-RX-API-DMAC7.html)

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CA2695264C (en) 2007-08-01 2015-01-27 Dana-Farber Cancer Institute Enrichment of a target sequence
US8206929B2 (en) * 2009-01-07 2012-06-26 Roche Molecular Systems, Inc. Nucleic acid amplification with allele-specific suppression of sequence variants
US20110129832A1 (en) 2009-10-27 2011-06-02 Swift Biosciences, Inc. Polynucleotide Primers and Probes
EP2545189B1 (en) * 2010-03-08 2018-01-10 Dana-Farber Cancer Institute, Inc. Full cold-pcr enrichment with reference blocking sequence
CN103080310A (zh) * 2010-09-10 2013-05-01 三菱化学美迪恩斯株式会社 使用光的核酸的扩增抑制方法和高灵敏度的选择性核酸扩增方法
EP3144396B1 (en) 2010-10-27 2020-01-01 President and Fellows of Harvard College Methods of use of toehold hairpin primer
JP2014507950A (ja) * 2011-02-28 2014-04-03 トランスゲノミック，インク． 混合個体群における標的ｄｎａを配列決定するためのキットおよび方法
US11130992B2 (en) 2011-03-31 2021-09-28 Dana-Farber Cancer Institute, Inc. Methods and compositions to enable multiplex COLD-PCR
WO2012151328A2 (en) 2011-05-02 2012-11-08 President And Fellows Of Harvard College Spatial sequestration of dynamic nucleic acid circuits
CN103635593B (zh) * 2011-05-04 2018-03-20 生物概念股份有限公司 用于检测核酸序列变体的方法
US9518292B2 (en) 2011-05-26 2016-12-13 Brandeis University Methods for suppression PCR
US9617392B2 (en) 2011-07-10 2017-04-11 President And Fellows Of Harvard College Compositions and methods for self-assembly of polymers with complementary macroscopic and microscopic scale units
KR102087062B1 (ko) 2011-09-16 2020-03-11 렉소겐 게엠베하 핵산 전사 방법
EP2570487A1 (en) 2011-09-16 2013-03-20 Lexogen GmbH Nucleic acid transcription method
US9260714B2 (en) * 2011-12-02 2016-02-16 Roche Molecular Systems, Inc. Suppression of non-specific amplification with high-homology oligonucleotides
US9133490B2 (en) 2012-05-16 2015-09-15 Transgenomic, Inc. Step-up method for COLD-PCR enrichment
EP2992110B1 (en) * 2013-04-29 2017-10-25 QIAGEN Marseille S.A.S. A method for dna amplification with a blocking oligonucleotide
CA2926722A1 (en) * 2013-10-19 2015-04-23 Trovagene, Inc. Detecting mutations in disease over time
ES2861353T3 (es) * 2014-05-19 2021-10-06 Univ Rice William M Amplificación específica de alelo utilizando una composición de oligonucleótidos cebadores no específicos de alelo y bloqueantes específicos de alelo solapantes
US12391985B2 (en) 2014-08-07 2025-08-19 Pharmassist Ltd Method of determining PIK3CA mutational status in a sample
PT3198026T (pt) 2014-08-07 2020-01-16 Pharmassist Ltd Método de determinação do estado mutacional de pik3ca numa amostra
HUE058219T2 (hu) 2014-09-26 2022-07-28 Janssen Pharmaceutica Nv FGFR-mutánsgénpanel alkalmazása olyan rákbetegek azonosításában, akik FGFR-inhibitorral történõ kezelésre reszponzívak lesznek
US9909169B2 (en) * 2014-12-17 2018-03-06 Roche Molecular Systems, Inc. Allele-specific amplification of nucleic acids using blocking oligonucleotides for wild type suppression
JP5813263B1 (ja) * 2015-03-31 2015-11-17 日鉄住金環境株式会社 遺伝子変異の検出方法及びそれに用いる蛍光標識オリゴヌクレオチド
US9657353B2 (en) 2015-06-26 2017-05-23 Great Basin Scientific, Inc. Specific detection of organisms derived from a sample
WO2017193025A1 (en) 2016-05-06 2017-11-09 William Marsh Rice University Stoichiometric nucleic acid purification using randomer capture probe libraries
JP7106461B2 (ja) * 2016-06-07 2022-07-26 セファイド 熱安定性ポリメラーゼ阻害剤組成物及び方法
WO2018111835A1 (en) 2016-12-12 2018-06-21 Dana-Farber Cancer Institute, Inc. Compositions and methods for molecular barcoding of dna molecules prior to mutation enrichment and/or mutation detection
WO2019023243A1 (en) 2017-07-24 2019-01-31 Dana-Farber Cancer Institute, Inc. METHODS AND COMPOSITIONS FOR SELECTING AND AMPLIFYING DNA TARGETS IN A SINGLE REACTION MIXTURE
WO2019099420A1 (en) * 2017-11-15 2019-05-23 Yan Wang A method for detecting multiple dna mutations and copy number variations
US12331350B2 (en) 2018-02-20 2025-06-17 William Marsh Rice University Systems and methods for allele enrichment using multiplexed blocker displacement amplification
KR102589502B1 (ko) 2018-07-19 2023-10-13 바이오피델리티 엘티디 개선된 폴리뉴클레오티드 서열 탐지 방법
EP3899036A1 (en) * 2018-12-17 2021-10-27 Illumina Cambridge Limited Primer oligonucleotide for sequencing
GB201919186D0 (en) 2019-12-23 2020-02-05 Biofidelity Ltd Simplified polynucleotide sequence detection method
JP2024531713A (ja) * 2021-09-13 2024-08-29 ネクスジェン キャンサー ディテクション エルエルシー 試料中の標的ポリヌクレオチドを検出するための方法

Family Cites Families (8)

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Publication number Priority date Publication date Assignee Title
CA2163393C (en) 1994-11-30 2003-04-22 Colleen Marie Nycz Amplification and detection of mycobacteria nucleic acids
US5801155A (en) * 1995-04-03 1998-09-01 Epoch Pharmaceuticals, Inc. Covalently linked oligonucleotide minor grove binder conjugates
US5849497A (en) * 1997-04-03 1998-12-15 The Research Foundation Of State University Of New York Specific inhibition of the polymerase chain reaction using a non-extendable oligonucleotide blocker
FR2779154B1 (fr) 1998-05-27 2002-07-12 Bio Merieux Procede d'amplification d'au moins une sequence nucleotidique particuliere et amorces de mise en oeuvre
JP2002534098A (ja) * 1999-01-06 2002-10-15 コーネル リサーチ ファンデーション インク． ゲノムシークエンシングにおける単一ヌクレオチドの多型の加速度的な同定およびクローンの整列化
EP1253205A1 (en) 2001-04-24 2002-10-30 LION Bioscience AG Method of blocking amplification of selected sequences
GB0406863D0 (en) 2004-03-26 2004-04-28 Qiagen As Nucleic acid sequencing
DK1794173T3 (da) 2004-09-10 2010-10-25 Human Genetic Signatures Pty Amplifikationsblokker indeholdende interkalaterende nukleinsyrer (INA) indeholdende interkalaterende pseudonukleotider (IPN)

Also Published As

Publication number Publication date
EP2173910A1 (en) 2010-04-14
US8071338B2 (en) 2011-12-06
CA2693384A1 (en) 2009-02-12
HK1146094A1 (zh) 2011-05-13
EP2173910B1 (en) 2015-12-09
JP2010535480A (ja) 2010-11-25
WO2009019008A1 (en) 2009-02-12
CN101778952B (zh) 2016-05-25
JP5524838B2 (ja) 2014-06-18
US20090053720A1 (en) 2009-02-26
CN101778952A (zh) 2010-07-14
ES2562789T3 (es) 2016-03-08

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