CN101773081B - Method for selecting and identifying paddlefish fertilized egg and dedicated egg pipette thereof - Google Patents
Method for selecting and identifying paddlefish fertilized egg and dedicated egg pipette thereof Download PDFInfo
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- CN101773081B CN101773081B CN2010101005644A CN201010100564A CN101773081B CN 101773081 B CN101773081 B CN 101773081B CN 2010101005644 A CN2010101005644 A CN 2010101005644A CN 201010100564 A CN201010100564 A CN 201010100564A CN 101773081 B CN101773081 B CN 101773081B
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- egg
- paddlefish
- fertilized egg
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention belongs to the technical field of artificial fish breeding, particularly relates to a technical method for selecting and identifying paddlefish fertilized egg by using an egg pipette and a dedicated egg pipette thereof. The method for selecting and identifying the paddlefish fertilized egg comprises the following steps: 1) selecting the fertilized egg from the paddlefish embryonic development to the nerviduct closing stage by using a straight pipe glass egg pipette; 2) rejecting abnormal developmental bad eggs; and 3) putting normal developmental fertilized eggs in a hatcher to hatch fish fry. The dedicated egg pipette is the straight pipe glass egg pipette, comprises a rubber bulb and a glass pipe, the diameter of the rubber bulb is 4-5cm, the glass pipe is round, the length of the glass pipe is 30cm, the inner diameter thereof is 0.5cm, and the mouth of the glass pipe is a plain end. The method and the device of the invention have simple operation, low production cost and good application prospect in artificial propagation of the paddlefish.
Description
Technical field
The invention belongs to the artificial propagation of fish technology, relate in particular to a kind of method and dedicated suction oviduct of selecting and differentiating paddlefish fertilized egg.
Background technology
China's inland waters aboundresources, the gross area have 1702.53 ten thousand hectares, and wherein the pond area is 192.16 ten thousand hectares, 752.43 ten thousand hectares of area of lake, 230.16 ten thousand hectares of reservoir areas, 527.78 ten thousand hectares of rivers areas.But the fish of putting in a suitable place to breed in all kinds of water bodys are more single, and not only the solid space of water body and food resource do not obtain reasonable use, and fish quality and culture benefit are also lower.Therefore, increasing the inland waters new quality variety puts in a suitable place to breed China lake, reservoir and pond fish stocking structural adjustment, improves that to put economic benefit in a suitable place to breed be vital.
Paddlefish is a kind of large-scale economic freshwater fish,, growth strong to environmental suitability rapidly, zooplankton mainly ingests.Fish-egg, the flesh of fish and fish-skin all have very high economic worth, are a kind of new quality varieties that China's inland waters are put in a suitable place to breed that is very suitable for.Paddlefish nineteen ninety is introduced China from the U.S., and after this annual China also needs to buy a large amount of fertilized eggs from the U.S., not only consumes a large amount of manpowers and foreign exchange, and its fertilized egg quality also can not get guaranteeing.The subject matter that the artificial propagation of present domestic paddlefish exists is that fertilized egg and unfertilized egg are not easily separated; And the last parasitism of unfertilized egg of the closed phase of refreshing spermaduct of embryonic development goes out a large amount of water mo(u)lds; Badly influence the fertilized egg of normal development; Cause the hatchability of fertile eggs of paddlefish normal development low, the quantity of emerging is few, and fry, fingerling can not satisfy the needs in the production far away.
Common way before the present invention proposes is; After the paddlefish fertilized egg unsticking, put into incubator and hatch, in hatching process; The ovum of unfertilized ovum and stasi can consume a large amount of oxygen; And be easy to generate water mo(u)ld, cause the ovum of normal development to stick together agglomerating, final downright bad at short notice.So, should in the time can distinguishing fertilized egg and unfertilized egg, the fertilized egg of normal development be chosen, continue hatching, could guarantee incubation rate and emergence rate.
Summary of the invention
The objective of the invention is to overcome existing paddlefish artificial propagation techniques and have technological deficiency; According to the closed phase characteristic of paddlefish embryonic development to refreshing spermaduct; Develop a kind of method and dedicated suction oviduct thereof of selecting and differentiating paddlefish fertilized egg; Method of the present invention and dedicated suction oviduct can be differentiated paddlefish fertilized egg apace, and fertilized egg picking out carried out artificial incubation, have solved the low and low problem of emergence rate of paddlefish hatchability of fertile eggs.
The present invention realizes through following technical scheme:
A kind of method of selecting and differentiating paddlefish fertilized egg, according to following steps:
1) inhales oviduct with straight pipe type glass, pick out paddlefish embryonic development to the refreshing spermaduct fertilized egg of closed phase;
2) the bad ovum of the improper growth of rejecting;
3) place incubator to hatch fry the normal development fertilized egg of selecting.
Described straight pipe type glass is inhaled oviduct can once draw paddlefish fertilized egg 25-30 grain.
The applicant has designed a kind of straight pipe type glass and has inhaled oviduct, and this suction oviduct is that a kind of straight pipe type glass is inhaled oviduct, and it comprises rubber bulb and glass tube two parts; Wherein said rubber bulb diameter is 4.5cm, and glass tube is circular, and it is long to be 30cm; Internal diameter is 0.5cm, and the mouth of pipe is flat mouthful.
Method of the present invention and dedicated suction oviduct are easy and simple to handle, and production cost is low, in the artificial propagation of paddlefish, have good application prospects.
Description of drawings
Fig. 1: the dorsal view that is the closed phase of refreshing spermaduct of paddlefish embryonic development.When 17 ℃～22 ℃ of water temperatures, paddlefish fertilized egg was grown to the closed phase of refreshing spermaduct through 47～48 hours, and neural fold is closed into nerve channel fully.
Fig. 2: the closed phase tail norma lateralis of refreshing spermaduct that is the paddlefish embryonic development.When 17 ℃～22 ℃ of water temperatures, fertilized egg was grown to the closed phase of refreshing spermaduct through 47～48 hours, and neural fold is closed into nerve channel fully.
Fig. 3: the dedicated suction oviduct that is the paddlefish fertilized egg discrimination method made of the present invention.Comprise rubber bulb and glass straight tube two minutes, wherein the rubber bulb diameter is 4.5cm, and the long 30cm of glass straight tube, spout diameter are 0.6cm.
Embodiment
Embodiment 1
Concrete steps of the present invention are: earlier paddlefish fertilized egg is hatched under 17 ℃～22 ℃ conditions of water temperature; Through hatching in 47～48 hours; The paddlefish embryo can grow to the closed phase of refreshing spermaduct of biological property (can see tangible distinguishing mark; Referring to illustrated in figures 1 and 2), be to distinguish the paddlefish feritilization of ovum and unfertilized sign phase and optimal viewing to differentiate period at this moment.Paddlefish unfertilized egg in this period then can not grow the closed phase of above-mentioned refreshing spermaduct.
More detailed method of operating is following: at first the paddlefish fish-egg in the incubator is taken out some and be placed in the white dissecting pan; Add clear water; Be advisable just to cover fish-egg, inhale oviduct with in the fertilized egg suction tube with the special-purpose straight pipe type glass of the present invention design again, with closed phase of refreshing spermaduct of fertilized egg be sign; Through the air pressure of control in the rubber bulb reject can not the normal development paddlefish bad ovum, stay the fertilized egg of normal development.The special-purpose straight pipe type glass that the present invention makes is inhaled oviduct can once suck paddlefish fertilized egg 25-30 grain, and everyone per hour can differentiate and select paddlefish fertilized egg 500-600 grain, so just can soon fertilized egg be picked out accurately.Then the fertilized egg of the normal development of picking out is put into another incubator (incubator is the cylinder that fills running water or the container of common usefulness) and hatch, this has just improved paddlefish fertilized egg hatching rate and emergence rate greatly.
The discriminating that the present invention makes and select the dedicated suction oviduct of paddlefish fertilized egg; Inhale oviduct for a kind of straight pipe type glass, this pipe comprises rubber bulb and glass tube two parts, and wherein said rubber bulb diameter is 4.5cm; Described straight pipe type glass tube is circular; Length is 30cm, and bore is 0.5cm in the glass tube, and the mouth of pipe is flat mouthful.
Straight pipe type glass of the present invention is inhaled the fertilized egg with paddlefish that oviduct can be efficient, easy and is chosen.Its outstanding advantage is: straight pipe type glass is inhaled the caliber suitable size of oviduct, and the fertilized egg of the paddlefish that can fully guarantee to suck is not overlapping, do not push, and can the ovum grain of paddlefish normal fertilization ovum and unfertilized egg clearly be separated; Have amplification voluntarily after the dedicated suction oviduct suction simultaneously of the present invention, can identify the characteristic of fertilized egg more clearly.The air pressure size of rubber bulb can be controlled the quantity of drawing paddlefish fertilized egg, guarantees that in selecting the process of fertilized egg, the tube wall of dedicated suction oviduct is smooth, can not cause the damage to paddlefish fertilized egg.
Claims (1)
1. method of selecting and differentiating paddlefish fertilized egg, according to following steps:
1) inhales oviduct with straight pipe type glass, pick out paddlefish embryonic development to the refreshing spermaduct fertilized egg of closed phase;
2) the bad ovum of the improper growth of rejecting;
3) place incubator to hatch fry the normal development fertilized egg of selecting;
Wherein: the described suction oviduct of step 1) comprises rubber bulb and glass tube two parts, and wherein the rubber bulb diameter is 4.5cm, and glass tube is circular, and it is long to be 30cm, and internal diameter is 0.5cm, and the mouth of pipe is flat mouthful.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010101005644A CN101773081B (en) | 2010-01-22 | 2010-01-22 | Method for selecting and identifying paddlefish fertilized egg and dedicated egg pipette thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010101005644A CN101773081B (en) | 2010-01-22 | 2010-01-22 | Method for selecting and identifying paddlefish fertilized egg and dedicated egg pipette thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101773081A CN101773081A (en) | 2010-07-14 |
| CN101773081B true CN101773081B (en) | 2012-06-27 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010101005644A Expired - Fee Related CN101773081B (en) | 2010-01-22 | 2010-01-22 | Method for selecting and identifying paddlefish fertilized egg and dedicated egg pipette thereof |
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| Country | Link |
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| CN (1) | CN101773081B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103081841B (en) * | 2013-03-05 | 2016-08-03 | 中国水产科学研究院黑龙江水产研究所 | Salmon fishes selects ovum method automatically |
| CN104823894A (en) * | 2015-06-03 | 2015-08-12 | 铜陵县国斌水产综合养殖有限责任公司 | Paddlefish breeding method |
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2010
- 2010-01-22 CN CN2010101005644A patent/CN101773081B/en not_active Expired - Fee Related
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| Publication number | Publication date |
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| CN101773081A (en) | 2010-07-14 |
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| C14 | Grant of patent or utility model | ||
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| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20120627 Termination date: 20130122 |