CN101768515B - Preparation method and application of natural extractive - Google Patents
Preparation method and application of natural extractive Download PDFInfo
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- CN101768515B CN101768515B CN2010100301547A CN201010030154A CN101768515B CN 101768515 B CN101768515 B CN 101768515B CN 2010100301547 A CN2010100301547 A CN 2010100301547A CN 201010030154 A CN201010030154 A CN 201010030154A CN 101768515 B CN101768515 B CN 101768515B
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Abstract
The invention discloses a preparation and an application of a natural extractive, characterized in that the extractive is prepared with the following method that mixed raw materials are crushed, sterilized by steam, enzyme-hydrolyzed and fermented, extracted by ethanol, filtered and condensed to become the extractive, wherein, the mixed raw materials include strawberry, cherry, apricot, mulberry, medlar, ebony, Cherry tomato and dogberry. If applied to the cigarette cut tobacco, the extractive of the invention can endow the cigarette with brewed sour-sweet fragrance, obviously improve mouth feel and aftertaste and reduce stimulation and impure smell. The application of the technology does not change the existing processing technology of cigarettes and can improve the internal quality of cigarettes. The invention has the beneficial effects that the raw materials come from plentiful sources; the zymohydrolysis and fermentation method is simple and convenient and the extraction method and technology is simple; the extraction with ethanol is right after the zymohydrolysis and fermentation, so the enzyme killing step is omitted; the ethanol solution is recyclable, not causing solution pollution in the product preparation process.
Description
Technical field
The present invention relates to the extraction and the application of biotechnology and natural perfume; Be specifically related to a kind of extraction using alcohol through the mixing dry fruit extract of enzymatic hydrolysis and fermentation and the application in perfuming cigarette thereof, the extract that utilizes the present invention to obtain has good action effect in perfuming cigarette.
Background technology
Essence and flavoring agent is widely used in daily chemical products, food, tobacco, feed and many Industrial products, and natural perfume is because its composition is complicated, fragrance is abundant, fragrance remaining time is the indispensable raw material of preparing essence lastingly.
The dry fruit of strawberry, cherry, apricot, mulberries, matrimony vine, dark plum, holy girl fruit, skunk bush all is sour-sweet fruit of taste or Chinese medicine, and they are nutritious, and are very popular, is eaten in many ways or is used as Chinese medicine.These materials contain the active chemical components of anti-oxidant antifatigue mostly, also can reduce the harm that cigarette smoke produces.
Summary of the invention
The object of the invention just is being based on above-mentioned condition and the preparation method and the application thereof of special a kind of natural extract of developing; Promptly, again this extract is applied on the cigarette shreds, can gives the sour-sweet fragrance of brewageing of cigarette grace through the mixing dry fruit extract of extraction using alcohol behind enzymatic hydrolysis and fermentation; Significantly improve the mouthfeel pleasant impression; Reduce to stimulate, assorted gas, and this The Application of Technology do not change the existing complete processing of cigarette, can improve the interior quality of cigarette.
The objective of the invention is to realize: the preparation method of natural extract of the present invention through following scheme; May further comprise the steps: mixing raw material → pulverizing → wet sterilization → enzymatic hydrolysis and fermentation → extraction using alcohol → filtration → concentrate → extract, said mixing raw material is made up of following materials of weight proportions:
Strawberry 2-8 part
Cherry 2-6 part
Apricot 2-5 part
Mulberries 1-4 part
Matrimony vine 1-5 part
Dark plum 2-5 part
Holy girl fruit 1-4 part
Skunk bush 1-3 part.
In the present invention, after the raw material pulverizing with 100 ℃ of wet sterilizations.Contain deleterious bacterial classifications such as mould in the raw material, kill deleterious bacterial classification such as mould through high temperature and prevent destruction that follow-up enzymatic hydrolysis and fermentation is produced.
Cross the Cheng Qian at enzymatic hydrolysis and fermentation; Adding water adjustment pulverizing back raw material water cut is 30-60%; The cellulase, the hemicellulase of 0.1-0.3%, 0.1-0.3% polygalacturonase, 0.01-0.05% AMS, the beta-glucosidase of 0.01-0.05%, the proteolytic enzyme of 0.01-0.05%, the 0.01-0.2% aroma-producing yeast bacterium that add 0.1-0.3% by raw material weight respectively; Above-mentioned enzyme and aroma-producing yeast bacterium water wiring solution-forming evenly spray are added on the raw material enzymatic hydrolysis and fermentation 2-48h under 40-70 ℃ of condition.After enzyme is handled; Be able to be degraded into small-molecule substance with influencing the macromolecular substance such as Mierocrystalline cellulose, semicellulose, pectin, starch and protein that flue gas causes occurring after the result of combustion of tobacco sticking with paste peculiar smell such as burnt gas, xylon gas, albumen empyreuma gas; Change inherent the composition, produced more micromolecular carbohydrate and amino acid.After the fragrant saccharomycetes to make fermentation of multiparity is handled, produce a lot of new aroma components.Because macromolecular substance such as pectin, starch are degraded to small-molecule substance, be easier to extract and dissolving.
In the extraction using alcohol process, alcohol concn is 60-95%, and raw material and ethanol weight ratio are 1: 6-1: 20, and extraction time is 2-24h, extraction temperature is 25-80 ℃.
The application of natural extract of the present invention is that extract is added in the cigarette shreds after with 95% dissolve with ethanol, can give cigarette graceful brewage sour-sweet fragrance, improve the mouthfeel pleasant impression, reduce stimulate, assorted gas.The concrete consumption of extract is the 0.01-0.3% of pipe tobacco weight.
Effect of the present invention and benefit are: raw materials used source is abundant, and enzymolysis, fermentation process is easy, process for extracting technology is simple, easy and simple to handle, and enzymatic hydrolysis and fermentation finishes and carries out extraction using alcohol immediately, has reduced the enzyme step of going out.Etoh solvent can recycle and reuse, and there is not solvent contamination in product.Extract fragrance exquisiteness of the present invention, the sour-sweet fragrance of brewageing with grace, the perfuming effect is remarkable in the tobacco, for tobacco provides novel, a respond well natural perfume.
Embodiment
Through embodiment method afford of the present invention is further explained below, but do not limited the present invention.
Embodiment 1
With electronic balance weighing strawberry 50g, cherry 40g, apricot 30g, mulberries 15g, structure Qi 15g, dark plum 20g, holy girl fruit 15g, skunk bush 15g; Pulverize; With 100 ℃ of steam heating sterilizations; Adding water adjustment raw material water cut then is 60%; Be added on raw material, enzymatic hydrolysis and fermentation 48h under 40 ℃ of conditions to the even spray of proteolytic enzyme, 0.2% (being 0.4g) the aroma-producing yeast bacterium wiring solution-forming of the beta-glucosidase, 0.05% (being 0.1g) of the hemicellulase, 0.3% of the cellulase, 0.3% of raw material weight 0.3% (being 0.6g) (being 0.6g) (being 0.6g) polygalacturonase, 0.05% (being 0.1g) AMS, 0.05% (being 0.1g).Adding 95% ethanol 4000g then, is to soak 24h under 25 ℃ of conditions in temperature, filters then, concentrates and to obtain extract 12g.
Take by weighing extract by 0.01% of pipe tobacco weight, after the dilution of 95% dissolve with ethanol, spray is added in the flue-cured tobacco pipe tobacco and rolls into cigarette; Think through smokeing panel test: this product can be coordinated with the cigarette cigarette is fragrant; Give the sour-sweet fragrance of brewageing of cigarette grace, the mouthfeel pleasant impression improves bigger, and the gas that stimulates, mixes reduces.
Embodiment 2
With electronic balance weighing strawberry 20g, cherry 15g, apricot 12g, mulberries 13g, matrimony vine 10g, dark plum 15g, holy girl fruit 10g, skunk bush 5g; Pulverize; With 100 ℃ of steam heating sterilizations; Adding water adjustment raw material water cut then is 30%; Be added on raw material, enzymatic hydrolysis and fermentation 2h under 70 ℃ of conditions to the even spray of proteolytic enzyme, 0.01% (being 0.01g) the aroma-producing yeast bacterium wiring solution-forming of the beta-glucosidase, 0.01% (being 0.01g) of the AMS, 0.01% (being 0.01g) of the hemicellulase, 0.1% of the cellulase, 0.1% of raw material weight 0.1% (being 0.1g) (being 0.1g) (being 0.1g) polygalacturonase, 0.01% (being 0.01g).Adding concentration then is 60% ethanol 600g, is 80 ℃ in temperature, and lixiviate 2h filters then, distills, concentrates and obtain extract 8g.
Take by weighing extract by 0.3% of pipe tobacco weight, after the dilution of 95% dissolve with ethanol, spray is added in the flue-cured tobacco pipe tobacco and rolls into cigarette; Think through smokeing panel test: this product and cigarette cigarette are fragrant to be coordinated; Give the sour-sweet fragrance of brewageing of cigarette grace, the mouthfeel pleasant impression improves, and the gas that stimulates, mixes reduces.
Claims (3)
1. the preparation method of a natural extract; It is characterized in that: this preparation method may further comprise the steps: mixing raw material → pulverizing → wet sterilization → enzymatic hydrolysis and fermentation → extraction using alcohol → filtration → concentrate → extract, and said mixing raw material is made up of following materials of weight proportions:
Strawberry 2-8 part,
Cherry 2-6 part,
Apricot 2-5 part,
Mulberries 1-4 part,
Matrimony vine 1-5 part,
Dark plum 2-5 part,
Holy girl fruit 1-4 part,
Skunk bush 1-3 part;
In the enzymatic hydrolysis and fermentation process; Adding water adjustment pulverizing back raw material water cut is 30-60%; The cellulase, the hemicellulase of 0.1-0.3%, 0.1-0.3% polygalacturonase, the AMS of 0.01-0.05%, the beta-glucosidase of 0.01-0.05%, the proteolytic enzyme of 0.01-0.05%, the 0.01-0.2% aroma-producing yeast bacterium that add 0.1-0.3% by raw material weight respectively; Above-mentioned enzyme and fungus matter water wiring solution-forming evenly spray are added on the raw material enzymatic hydrolysis and fermentation 2-48h under 40-70 ℃ of condition;
In the extraction using alcohol process, alcohol concn is 60-95%, and raw material and ethanol weight ratio are 1: 6-1: 20, and extraction time is 2-24h, extraction temperature is 25-80 ℃.
2. the preparation method of a kind of natural extract according to claim 1 is characterized in that: after the raw material pulverizing with 100 ℃ of wet sterilizations.
3. the application of the described natural extract of claim 1; It is characterized in that: extract is added in the cigarette shreds after with 95% dissolve with ethanol; Can give the sour-sweet fragrance of brewageing of cigarette grace; Improve the mouthfeel pleasant impression, reduce the gas that stimulates, mixes, the concrete consumption of extract is the 0.01-0.3% of pipe tobacco weight.
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| CN101768515B true CN101768515B (en) | 2012-08-22 |
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