CN106753796A - A kind of preparation method and applications of fruit aroma extract - Google Patents
A kind of preparation method and applications of fruit aroma extract Download PDFInfo
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- CN106753796A CN106753796A CN201611241263.7A CN201611241263A CN106753796A CN 106753796 A CN106753796 A CN 106753796A CN 201611241263 A CN201611241263 A CN 201611241263A CN 106753796 A CN106753796 A CN 106753796A
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B9/00—Essential oils; Perfumes
- C11B9/02—Recovery or refining of essential oils from raw materials
-
- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B3/00—Preparing tobacco in the factory
- A24B3/12—Steaming, curing, or flavouring tobacco
-
- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24D—CIGARS; CIGARETTES; TOBACCO SMOKE FILTERS; MOUTHPIECES FOR CIGARS OR CIGARETTES; MANUFACTURE OF TOBACCO SMOKE FILTERS OR MOUTHPIECES
- A24D3/00—Tobacco smoke filters, e.g. filter-tips, filtering inserts; Filters specially adapted for simulated smoking devices; Mouthpieces for cigars or cigarettes
- A24D3/06—Use of materials for tobacco smoke filters
- A24D3/14—Use of materials for tobacco smoke filters of organic materials as additive
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B9/00—Essential oils; Perfumes
- C11B9/02—Recovery or refining of essential oils from raw materials
- C11B9/022—Refining
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- Chemical Kinetics & Catalysis (AREA)
- Oil, Petroleum & Natural Gas (AREA)
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Abstract
The application is related to tobacco additive agent field, specifically, is related to a kind of preparation method and applications of fruit aroma extract.Preparation method is prepared and comprised the following steps：Fresh plant fruit is crushed, slurry is made；Add complex enzyme to be hydrolyzed in the slurry, protease and carbohydrate hydrolase are contained in complex enzyme, protease is 1 with the mass ratio of carbohydrate hydrolase：15~60, by hydrolysate is purified and concentration obtains final product fruit aroma extract.The application is digested by using specific biology enzyme to fresh fruit, effectively quick broken wall, promotion macromolecular substances are converted and dispel impurity, when the fruit aroma extract that the preparation method of the application is obtained is used for cigarette, cigarette perfume can be enriched, increase sweet sense, and cigarette sense organ humectation, comprehensive evaluation index is good.And the preparation process is simple of the application, has a wide range of application, it is suitable to large-scale promotion and uses.
Description
Technical field
The application is related to tobacco additive agent field, specifically, be related to a kind of preparation method of fruit aroma extract and its
Using.
Background technology
Cigarette flavouring humectation is the key technology for promoting Chinese-style cigarette sustainable and healthy development, for lifting Chinese-style cigarette product
Matter, enhance one's market competitiveness it is significant.Improve the aroma characteristic and comfortableness of cigarette product, highlight brand style, one
It has been striving direction that Cigarette Manufacturing Industry is seek assiduously since straight.In the research of report, most fruits extract is all preferable
Essence spice for cigarette perfume material, being added in cigarette shreds can play sweetened, flavouring effect, but can bring thorn
Swash, residual and the adverse effect such as flue gas is coarse.
Therefore, special propose the application.
The content of the invention
The primary goal of the invention of the application is to propose a kind of preparation method of fruit aroma extract.
Second goal of the invention of the application is to propose the application of the preparation method.
In order to complete the purpose of the application, the technical scheme for using for：
The present invention relates to a kind of preparation method of fruit aroma extract, comprise the following steps：
(1) fresh plant fruit is crushed, is made slurry；
(2) add complex enzyme to be hydrolyzed in the slurry, protease and carbohydrate hydrolase contained in the complex enzyme,
The carbohydrate hydrolase is selected from least one in cellulase, pectase, glucuroide, dextranase, protease and sugar
The mass ratio of class hydrolase is 1：15~60；
(3) by hydrolysate is purified and concentration obtains final product the fruit aroma extract.
Preferably, the fruit is selected from stone fruit or berry fruits；The preferred cherry of stone fruit, it is described
The preferred mulberries of berry fruits.
Preferably, protease, pectase and glucuroide are contained in the complex enzyme；Preferably, protease and pectin
The mass ratio of enzyme is 1：1~8.
Preferably, at least one in cellulase and dextranase is also contained in the complex enzyme, it is preferred that cellulose
The mass ratio of at least one and protease in enzyme and dextranase is 2.5~4：1.
Preferably, in step (1), the solid content of the slurry is 10~20%.
Preferably, in step (2), the addition of the complex enzyme is the 0.01%~1.0% of the stock quality.
Preferably, in step (2), the temperature during hydrolysis is 30~80 DEG C, and the time of the hydrolysis is 2~48 small
When.
Preferably, in step (3), the method for the purification is alcohol precipitation, it is preferred that the condition of alcohol precipitation is：After hydrolyzing
Product filter to get filtrate, add the ethanol of 1~6 times of the filtrate volume, 8~24 are then stood at a temperature of -10 DEG C~5 DEG C
Hour, wherein, the concentration of volume percent of ethanol is 10%~95%.
Preferably, in step (3), the method for the concentration is concentrated under reduced pressure, it is preferred that pressure concentrated under reduced pressure for-
0.1~-0.08MPa, temperature when being concentrated under reduced pressure is 38~47 DEG C.
The application further relates to the application of fruit aroma extract that the application preparation method prepares in cigarette, preferably
, the fruit aroma extract makes an addition at least one in pipe tobacco, cigarette paper, filter stick；It is furthermore preferred that the fruit aroma
Mass percentage content when extract is made an addition in pipe tobacco is 0.01%~0.5%, and the fruit aroma extract makes an addition to
Mass percentage content during cigarette paper is 0.05%~1.0%, and the fruit aroma extract makes an addition to quality during filter stick
Degree is 0.02%~2%.
The technical scheme of the application at least has following beneficial effect：
The application is digested by using specific biology enzyme to fresh fruit, gives full play to the single-minded, efficient of enzyme, temperature
With and the performance such as activity is adjustable, so as to can reach broken wall, upgrading and the effect of removal of impurities, effectively quick broken wall, promote big point
Sub- material is converted and dispels impurity, can improve the purity and yield of extract.
The fruit aroma extract that the preparation method of the application is prepared can enrich cigarette perfume, increase sweet sense, and cigarette sense
Official's humectation, uses《Sensory comfort of cigarette evaluation method》The method and index of (YC/T 496-2014) regulation are evaluated, and are adopted
After the fruit aroma extract prepared with the application preparation method makes an addition to cigarette, its comprehensive evaluation index is good.
And the preparation process is simple of the application, has a wide range of application, it is suitable to large-scale promotion and uses.
Specific embodiment
With reference to specific embodiment, the application is expanded on further.It should be understood that these embodiments are merely to illustrate the application
Rather than limitation scope of the present application.
The application is related to a kind of preparation method of fruit aroma extract, and specifically, the fruit in the application may be selected from core
Fruit fruit or berry fruits；Wherein, in stone fruit cherry rich in sugar, protein, crude fibre, vitamin, various
Amino acid and mineral matter etc., with middle regulating and qi tonifying, strengthening the spleen and stomach, wind-damp dispelling, the multi-efficiency such as beautifying face and moistering lotion, and because of its uniqueness
Aromatic odor and enjoy and like, therefore the preferred cherry of stone fruit in this application；And in berry fruits, the battalion of mulberry fruit
Support abundant, and the traditional Chinese medical science is additionally considered that mulberry fruit has and tonifies the liver and kidney, nourishing yin and nourishing blood, effect of ceases wind.Therefore berry in this application
The preferred mulberries of class fruit.
The preparation method of the application is prepared from fresh fruit, this is because, the direction class material in fresh fruit
Content highest, in fruit storage or drying course, aromatic substance volatilization is serious, causes the aroma extract matter of acquisition
Quality decreases.
The preparation method of the application is prepared and comprised the following steps：
First, fresh plant fruit is crushed, is made slurry；In order that hydrolase preferably plays a role, it is preferred that slurry
The solid content of material is 10~20%；If the moisture of fruit is relatively low, the regulation to this Shen that adds water in the slurry is needed
Please in the range of.
Secondly, complex enzyme is added to be hydrolyzed in the slurry；Hydrolyzed containing protease and carbohydrate in the complex enzyme of the application
Enzyme；Carbohydrate hydrolase in this application refers to the enzyme that disaccharide or polysaccharide are hydrolyzed；It is chosen in particular from cellulase, pectin
At least one in enzyme, glucuroide, dextranase；Under the conditions of the complex enzyme of the application, not only by the carbohydrate in fruit
, can also be hydrolyzed into for the protein component in fruit many by the material such as substance decomposition such as pectin, cellulose, hemicellulose, lignin
Peptide and Free Amino Acids, when fruit aroma extract is applied to cigarette, can increase the sweet sense of its fruit, reduce flue gas
Sense of anxiety and harsh feeling, reduce stimulate and remain.
In this application, protease and the mass ratio of carbohydrate hydrolase are 1：15~60；Preferably, protease and carbohydrate water
The mass ratio for solving enzyme is 1：25~55.The application pass through experimental studies have found that, the complex enzyme in the application proportion is in water
After solution fresh fruit, the fruit aroma extract for being obtained is applied to that after cigarette cigarette perfume can be enriched, increases sweet sense and moisture feeling, makes comprehensive
Close sensory experience optimal.
Improved as one kind of the application preparation method, protease, pectase and glucuroide are contained in complex enzyme.Its
In, glucuroide is the big fermentoid in glycoside hydrolase extended familys, and major function is hydrolyzation of glucose glycosidic bond, discharges Portugal
Grape sugar is used as product；Pectase refers to the enzyme for decomposing pectic substance.
Improved as one kind of the application preparation method, the mass ratio of protease and pectase is 1：1~8.
As the application preparation method one kind improve, in complex enzyme also containing in cellulase and dextranase at least
One kind, with the further effect for increasing enzymolysis.
Improved as one kind of the application preparation method, at least one and protease in cellulase and dextranase
Mass ratio is 2.5~4：1.Within the range, the glucide in fruit can be further fully hydrolyzed, and is retained to greatest extent
Its fragrance matter.
Improved as one kind of the application preparation method, the addition of complex enzyme is the 0.01%~1.0% of stock quality,
Preferably 0.1~1.0%.Enzyme digestion reaction can not be completed fully if addition is too small, waste multiple if addition is excessive
Synthase raw material, and quality can be influenceed due to overreaction.
Improved as one kind of the application preparation method, temperature during hydrolysis is 30~80 DEG C, preferably 40~60 DEG C；Water
The time of solution is 2~48 hours, preferably 4~24 hours.
Finally, the application is purified by hydrolysate and concentration obtains final product fruit aroma extract.
Improved as one kind of the application preparation method, the method for purification is alcohol precipitation.The application uses the method for alcohol precipitation not
It is only capable of reaching the purpose of purification, inactivation can also be played a part of to enzyme.Therefore, special setting is no longer needed to heat in the application
Go out enzyme the step of, so as to simplify production technology.
Improved as one kind of the application preparation method, the condition of alcohol precipitation is：Product after hydrolysis is filtered to get filtrate, plus
Enter the ethanol of 1~6 times of filtrate volume, then stand 8~24 hours at a temperature of -10 DEG C~5 DEG C, wherein, the volume hundred of ethanol
Specific concentration is divided to be 10%~95%.The yield of extract is can further improve using the alcohol precipitation condition in the range of this.
Improved as one kind of the application preparation method, the method for concentration is concentrated under reduced pressure.
Improved as one kind of the application preparation method, pressure concentrated under reduced pressure is -0.1~-0.08MPa, is concentrated under reduced pressure
When temperature be 38~47 DEG C.The application employs low-temperature reduced-pressure concentration, so as to the fragrance of extract can be retained to greatest extent.
The application further relates to application of the preparation-obtained fruit aroma extract of the preparation method in cigarette.
Preferably, fruit aroma extract makes an addition at least one in pipe tobacco, cigarette paper, filter stick；Fruit aroma is extracted
Thing is made an addition in pipe tobacco, is capable of achieving to the humectation of cigarette sense organ, abundant cigarette perfume, is increased sweet sense, and stimulation, residual is reduced to greatest extent
Stay, the negative effect such as flue gas is coarse.When fruit aroma extract is made an addition in cigarette paper and/or filter stick, due to without with pipe tobacco
Mixing, therefore the fruity during consumer can smell cigarette paper and/or filter stick before the unignited cigarette, so as to increased consumer
Sensory experience.
It is further preferred that mass percentage content when fruit aroma extract is made an addition in pipe tobacco for 0.01%~
0.5%, mass percentage content when making an addition to cigarette paper is 0.05%~1.0%, makes an addition to mass percent during filter stick
Content is 0.02%~2%.If addition is too low, the fragrant and sweet sense in humectation effect and cigarette is not enough, if addition mistake
Greatly, then certain influence can be brought on pipe tobacco taste in itself.
Below by taking fresh cherry and mulberries as an example, the technical scheme of the application is described in detail.
The application is related to a kind of preparation method of cherry extract, comprises the following steps：
First, fresh cherry is crushed, is made cherry slurry, the solid content of cherry slurry is 10~20%.
Secondly, complex enzyme is added to be hydrolyzed in cherry slurry；Contain protease and carbohydrate hydrolase in complex enzyme；Egg
White enzyme is 1 with the mass ratio of carbohydrate hydrolase：15~60；Preferably, protease and the mass ratio of carbohydrate hydrolase are 1：25~
55.The application pass through experimental studies have found that, complex enzyme in the application proportion after cherry is hydrolyzed, the cherry for being obtained
Extract is applied to that after cigarette cigarette perfume can be enriched, increases sweet sense and moisture feeling, integrated sensory is experienced optimal.
Preferably, protease, pectase and glucuroide are contained in complex enzyme.It is furthermore preferred that protease and pectase
Mass ratio be 1：1~8, and further preferred 1：2~6, more preferably 1：2.5~5.Wherein, the enzyme activity for using protease is 5
Ten thousand U/g, it is 50U/g to use pectase enzyme activity, and glucuroide enzyme activity is 100,000 U/ml.
Preferably, digested with further increase also containing at least one in cellulase and dextranase in complex enzyme
Effect.The enzyme activity of used cellulase is 1.5 ten thousand U/g, and the enzyme activity for using dextranase is 200U/g.
Preferably, the mass ratio of at least one and protease in cellulase and dextranase is 2.5~4：1, preferably 3
~4：1.
Preferably, the addition of complex enzyme is the 0.01%~1.0% of stock quality, preferably 0.1~1.0%.
Preferably, temperature during hydrolysis is 30~60 DEG C, preferably 40~50 DEG C；The time of hydrolysis is 4~24 hours, excellent
Elect as 4~6 hours.
Finally, the application is purified by hydrolysate and concentration obtains final product cherry extract.
Wherein, the method for purification is alcohol precipitation, and the condition of alcohol precipitation is：Product after hydrolysis is filtered to get filtrate, filtrate is added
The ethanol that 3~6 times of volume, then stands 12~24 hours at a temperature of -10 DEG C~5 DEG C, and the concentration of volume percent of ethanol is
90%~95%.
Wherein, the method for concentration is that pressure concentrated under reduced pressure, concentrated under reduced pressure is -0.1~-0.08MPa, when being concentrated under reduced pressure
Temperature is 38~47 DEG C.The yield of cherry extract is 2~5% of dry matter weight in cherry slurry.
The application further relates to a kind of preparation method of mulberries extract, comprises the following steps：
First, fresh mulberries are crushed, is made mulberries slurry, the solid content of mulberries slurry is 10~20%.
Secondly, complex enzyme is added to be hydrolyzed in mulberries slurry；Contain protease and carbohydrate hydrolase in complex enzyme；Egg
White enzyme is 1 with the mass ratio of carbohydrate hydrolase：15~60；Preferably, protease and the mass ratio of carbohydrate hydrolase are 1：30~
40.The application pass through experimental studies have found that, complex enzyme in the application proportion after mulberries are hydrolyzed, the mulberries for being obtained
Extract is applied to that after cigarette cigarette perfume can be enriched, increases sweet sense and moisture feeling, integrated sensory is experienced optimal.
Preferably, protease, pectase and glucuroide are contained in complex enzyme.It is furthermore preferred that protease and pectase
Mass ratio be 1：1~8, and further preferred 1：2~3.Wherein, the enzyme activity for using protease is 5 × 104U/g, is used
Pectase enzyme activity is 50U/g, and glucuroide enzyme activity is 105U/ml.Preferably, it is also poly- containing cellulase and Portugal in complex enzyme
At least one in carbohydrase, with the further effect for increasing enzymolysis.The enzyme activity of used cellulase is 1.5 × 104U/g, institute
The enzyme activity for using dextranase is 200U/g.
Preferably, the mass ratio of at least one and protease in cellulase and dextranase is 2.5~4：1, preferably
2.5~3：1.
Preferably, the addition of complex enzyme is the 0.01%~1.0% of stock quality, preferably 0.1~1.0%.
Preferably, temperature during hydrolysis is 30~60 DEG C, preferably 40~50 DEG C；The time of hydrolysis is 4~24 hours, excellent
Elect as 4~6 hours.
Finally, the application is purified by hydrolysate and concentration obtains final product mulberries extract.
Wherein, the method for purification is alcohol precipitation, and the condition of alcohol precipitation is：Product after hydrolysis is filtered to get filtrate, filtrate is added
The ethanol that 3~6 times of volume, then stands 12~24 hours at a temperature of -10 DEG C~5 DEG C, and the concentration of volume percent of ethanol is
90%~95%.
Wherein, the method for concentration is that pressure concentrated under reduced pressure, concentrated under reduced pressure is -0.1~-0.08MPa, when being concentrated under reduced pressure
Temperature is 38~47 DEG C.The yield of mulberries extract is 2~5% of dry matter weight in mulberries slurry.
In addition to cherry and mulberries disclosed above, the preparation technology of the application can be additionally used in the extraction of other fruit, be limited to
Length is repeated no more.
Embodiment 1
(1) cherry that will be clean crusher in crushing 10 minutes, weighs 100 grams of cherries slurries and is placed in 250mL round-bottomed flasks；
(2) after adding proteinase-10 .02g, pectase 0.05g, glucuroide 1.0g, cellulase 0.07g, by round bottom
Flask is placed in 40 ± 1C ° of water-bath, stirring reaction 6 hours；Wherein, the enzyme activity of protease is 5 × 104U/g, pectase enzyme activity
It is 50U/g, glucuroide enzyme activity is 100,000 U/ml, and the enzyme activity of cellulase is 1.5 × 104U/g；
(3) after cooling, filter, take filtrate；
(4) 60g95% ethanol, under the conditions of -5 DEG C, natural subsidence 12 hours are added；
(5) filter, take filtrate, by filtrate pressure be -0.09 ± 0.01MPa, temperature be 40 ± 2 DEG C under the conditions of subtracted
Pressure concentration, makes concentrate proportion reach 1.000 ± 0.005g/cm3, as enzyme digestion reaction type cigarette cherry extract A.Wherein,
The yield of cigarette cherry extract A is 4.5% of dry matter weight in cherry slurry.
Embodiment 2：
(1) cherry that will be clean crusher in crushing 10 minutes, weighs 100 grams of cherries slurries and is placed in 250mL round-bottomed flasks；
(2) after adding proteinase-10 .02g, pectase 0.1g, glucuroide 0.15g, dextranase 0.07g, by round bottom
Flask is placed in 50 ± 1C ° of water-bath, stirring reaction 4 hours；Wherein, the enzyme activity of protease is 5 × 104U/g, pectase enzyme activity
It is 50U/g, glucuroide enzyme activity is 105U/ml, the enzyme activity of dextranase is 200U/g；
(3) after cooling, filter, take filtrate；
(4) 80g90% ethanol, under the conditions of -5 DEG C, natural subsidence 24 hours are added；
(5) filter, take filtrate, by filtrate pressure be -0.10 ± 0.01MPa, temperature be 45 ± 2 DEG C under the conditions of subtracted
Pressure concentration, makes concentrate proportion reach 1.000 ± 0.005g/cm3, as enzyme digestion reaction type cigarette cherry extract B.Wherein,
The yield of cigarette cherry extract B is 4.6% of dry matter weight in cherry slurry.
Embodiment 3：
(1) fresh mulberry fruit that will be clean crusher in crushing 10 minutes, weighs 100 grams of mulberry fruits slurries and is placed in 250mL round bottoms burning
In bottle；
(2) after adding proteinase-10 .03g, pectase 0.08g, glucuroide 0.9g, cellulase 0.08g, by round bottom
Flask is placed in 45 ± 1C ° of water-bath, stirring reaction 5 hours；Wherein, the enzyme activity of protease is 5 × 104U/g, pectase enzyme activity
It is 50U/g, glucuroide enzyme activity is 105U/ml, the enzyme activity of cellulase is 1.5 ten thousand U/g；
(3) after cooling, filter, take filtrate；
(4) 60g95% ethanol, under the conditions of -5 DEG C, natural subsidence 12 hours are added；
(5) filter, take filtrate, by filtrate pressure be -0.09 ± 0.01MPa, temperature be 40 ± 2 DEG C under the conditions of subtracted
Pressure concentration, makes concentrate proportion reach 1.000 ± 0.005g/cm3, as enzyme digestion reaction type cigarette Fructus Mori extract C.Wherein,
The yield of cigarette Fructus Mori extract C is 4.7% of dry matter weight in mulberry fruit slurry.
Embodiment 4：
(1) fresh mulberry fruit that will be clean crusher in crushing 10 minutes, weighs 100 grams of mulberry fruits slurries and is placed in 250mL round bottoms burning
In bottle；
(2) after adding proteinase-10 .03g, pectase 0.06g, glucuroide 0.8g, dextranase 0.09g, by round bottom
Flask is placed in 45 ± 1C ° of water-bath, stirring reaction 6 hours；Wherein, the enzyme activity of protease is 5 × 104U/g, pectase enzyme activity
It is 50U/g, glucuroide enzyme activity is 105U/ml, the enzyme activity of dextranase is 200U/g；
(3) after cooling, filter, take filtrate；
(4) 80g90% ethanol, under the conditions of -5 DEG C, natural subsidence 24 hours are added；
(5) filter, take filtrate, by filtrate pressure be -0.09 ± 0.01MPa, temperature be 40 ± 2 DEG C under the conditions of subtracted
Pressure concentration, makes concentrate proportion reach 1.000 ± 0.005g/cm3, as enzyme digestion reaction type cigarette Fructus Mori extract D.Wherein,
The yield of cigarette Fructus Mori extract D is 4.8% of dry matter weight in mulberry fruit slurry.
Experimental example 1：
The cherry extract A and B that embodiment 1 and 2 is prepared, in being separately added into pipe tobacco, be applied on cigarette paper and
Be added in filter stick, adding proportion refers to table 1, blank be separately added into identical deal distilled water (numbering be respectively CK11,
CK22、CK33).By the cigarette sensory evaluation expert (9 people) with sensory evaluation qualification, according to the industry mark of cigarette sense organ humectation
Accurate (sensory comfort of cigarette evaluation method YC/T 496-2014) is smoked panel test.Smoking result is see table 2.
Table 1：Cherry extract use ratio and numbering
Table 2：Cherry extract sensory evaluation effect
In summary analysis understands that the cherry extract of the application is in terms of the humectation of cigarette sense organ, improving cigarette comfortableness
Effect is obvious.
Embodiment 2
The mulberries extract A and B that embodiment 3 and 4 is prepared, in being separately added into pipe tobacco, be applied on cigarette paper and
Be added in filter stick, adding proportion refers to table 4, blank be separately added into identical deal distilled water (numbering be respectively CK11,
CK22、CK33).By cigarette sensory evaluation expert Blind Test (9 people) with sensory evaluation qualification, according to the row of cigarette sense organ humectation
Industry standard (sensory comfort of cigarette evaluation method YC/T 496-2014) is smoked panel test.Smoking result is see table 4.
Table 3：Extract use ratio and numbering
Table 4：Sensory evaluation effect
In summary analysis understands that the mulberries extract of the application is in terms of the humectation of cigarette sense organ, improving cigarette comfortableness
Effect is obvious.
Comparative example 1：
Using with embodiment 1 in carry out following contrast experiment with a batch of fruit raw material：
Method according to embodiment 1 prepares cherry extract, differs only in the cherry for without protease, preparing
Extract A D1；
Method according to embodiment 1 prepares cherry extract, differs only in and only adds protease, the cherry for preparing
Extract A D2；
Method according to embodiment 1 prepares cherry extract, and the composition for differing only in complex enzyme is：Proteinase-10 .55g,
Pectase 0.55g；The cherry extract AD3 for preparing；
Method according to embodiment 1 prepares cherry extract, and the composition for differing only in complex enzyme is：Proteinase-10 .4g,
Pectase 0.4g, cellulase 0.4g；The cherry extract AD4 for preparing；
Method according to embodiment 1 prepares cherry extract, and the composition for differing only in complex enzyme is：Proteinase-10 .2g,
Pectase 0.6g, cellulase 0.4g；The cherry extract AD5 for preparing；
Method according to embodiment 1 prepares cherry extract, and the composition for differing only in complex enzyme is：Proteinase-10 .02g,
Pectase 0.2g, glucuroide 0.8g, the cherry extract AD6 for preparing.
Cherry extract AD1~AD6 that above-mentioned comparative example is prepared, is separately added into pipe tobacco, and adding proportion is with real
Example 1 is tested, blank is separately added into the distilled water of identical deal, and numbering is CK11.By the cigarette sense with sensory evaluation qualification
Official evaluates expert (9 people) Blind Test, according to professional standard (the sensory comfort of cigarette evaluation method YC/T of cigarette sense organ humectation
496-2014) smoked panel test.Smoking result and yield are shown in Table 5.
Table 5：
It can be seen from result is commented in above-mentioned suction, best results are commented in the cherry extract obtained under the application preparation technology, its suction,
And change the technological parameter of the application in comparative example, and comment effect to bring harmful effect suction, it is the humectation of influence cigarette sense organ, comfortable
Effect in terms of property.
Comparative example 2：
Using with embodiment 1 in carry out following contrast experiment with a batch of fruit raw material：
Method according to embodiment 3 prepares mulberries extract, differs only in the mulberries for without protease, preparing
Extract C D1；
Method according to embodiment 1 prepares mulberries extract, differs only in and only adds protease, the mulberries for preparing
Extract C D2；
Method according to embodiment 1 prepares mulberries extract, and the composition for differing only in complex enzyme is：Proteinase-10 .55g,
Pectase 0.55g；The mulberries extract CD3 for preparing；
Method according to embodiment 1 prepares mulberries extract, and the composition for differing only in complex enzyme is：Proteinase-10 .4g,
Pectase 0.4g, cellulase 0.4g；The mulberries extract CD4 for preparing；
Method according to embodiment 1 prepares mulberries extract, and the composition for differing only in complex enzyme is：Proteinase-10 .2g,
Pectase 0.6g, cellulase 0.4g；The mulberries extract CD5 for preparing；
Method according to embodiment 1 prepares mulberries extract, and the composition for differing only in complex enzyme is：Proteinase-10 .02g,
Pectase 0.2g, glucuroide 0.8g, the mulberries extract CD6 for preparing.
Mulberries extract CD1~CD6 that above-mentioned comparative example is prepared, is separately added into pipe tobacco, and adding proportion is with real
Example 1 is tested, blank is separately added into the distilled water of identical deal, and numbering is CK11.By the cigarette sense with sensory evaluation qualification
Official evaluates expert's Blind Test (9 people), according to professional standard (the sensory comfort of cigarette evaluation method YC/T of cigarette sense organ humectation
496-2014) smoked panel test.Smoking result and yield are see table 6.
Table 6：
It can be seen from result is commented in above-mentioned suction, best results are commented in the mulberries extract obtained under the application preparation technology, its suction,
And change the technological parameter of the application in comparative example, and comment effect to bring harmful effect suction, it is the humectation of influence cigarette sense organ, comfortable
Effect in terms of property.
Comparative example 3
Using with embodiment 1 in carry out following contrast experiment with a batch of fruit raw material：
Method according to embodiment 1 prepares cherry extract, differs only in：In step 2, enzymolysis time is 2 hours,
The cherry extract AD7 for preparing；
Method according to embodiment 1 prepares cherry extract, differs only in：In step 2, enzymolysis time is 24 small
When, the cherry extract AD8 for preparing；
Method according to embodiment 1 prepares cherry extract, differs only in：In step 2, hydrolysis temperature is 60 DEG C,
The cherry extract AD9 for preparing；
Method according to embodiment 1 prepares cherry extract, differs only in：In step 4, the concentration of ethanol is
50%, the cherry extract AD10 for preparing；
Method according to embodiment 1 prepares cherry extract, differs only in：In steps of 5, temperature concentrated under reduced pressure is
50 DEG C, the cherry extract AD11 for preparing.
Cherry extract AD7~AD11 that above-mentioned comparative example is prepared, is separately added into pipe tobacco, and adding proportion is with real
Example 1 is tested, blank is separately added into the distilled water of identical deal, and numbering is CK11.By the cigarette sense with sensory evaluation qualification
Official evaluates expert (9 people), according to professional standard (the sensory comfort of cigarette evaluation method YC/T 496- of cigarette sense organ humectation
2014) smoked panel test.Smoking result and yield are shown in Table 7.
Table 7：
It can be seen from result and yield are commented in above-mentioned suction, the cherry extract obtained under the application preparation technology is not only inhaled and is commented
Best results, it is also ensured that yield, so that enterprise profit is improved, with important commercial value.And change this Shen in comparative example
Technological parameter please, not only comments effect to bring harmful effect suction, also results in the reduction of yield.
It is not for limiting claim, any this area skill although the application is disclosed as above with preferred embodiment
Art personnel are not being departed from the premise of the application conceives, and can make some possible variations and modification, therefore the application
The scope that protection domain should be defined by the application claim is defined.
Claims (10)
1. a kind of preparation method of fruit aroma extract, it is characterised in that the preparation method is prepared and comprised the following steps：
(1) fresh plant fruit is crushed, is made slurry；
(2) add complex enzyme to be hydrolyzed in the slurry, protease and carbohydrate hydrolase are contained in the complex enzyme, it is described
Carbohydrate hydrolase is selected from least one in cellulase, pectase, glucuroide, dextranase, protease and carbohydrate water
The mass ratio for solving enzyme is 1：15~60；
(3) by hydrolysate is purified and concentration obtains final product the fruit aroma extract.
2. preparation method according to claim 1, it is characterised in that the fruit is selected from stone fruit or berries water
Really；The preferred cherry of stone fruit, the preferred mulberries of berry fruits.
3. preparation method according to claim 1, it is characterised in that in the complex enzyme containing protease, pectase and
Glucuroide；Preferably, the mass ratio of protease and pectase is 1：1~8.
4. preparation method according to claim 1, it is characterised in that also poly- containing cellulase and Portugal in the complex enzyme
At least one in carbohydrase, it is preferred that the mass ratio of at least one and protease in cellulase and dextranase be 2.5~
4：1.
5. preparation method according to claim 4, it is characterised in that in step (1), the solid content of the slurry
It is 10~20%.
6. preparation method according to claim 1, it is characterised in that in step (2), the addition of the complex enzyme is
The 0.01%~1.0% of the stock quality.
7. preparation method according to claim 1, it is characterised in that in step (2), the temperature during hydrolysis is 30
~80 DEG C, the time of the hydrolysis is 2~48 hours.
8. preparation method according to claim 1, it is characterised in that in step (3), the method for the purification is alcohol
It is heavy, it is preferred that the condition of alcohol precipitation is：Product after hydrolysis is filtered to get filtrate, the ethanol of 1~6 times of the filtrate volume is added,
Then 8~24 hours are stood at a temperature of -10 DEG C~5 DEG C, wherein, the concentration of volume percent of ethanol is 10%~95%.
9. preparation method according to claim 1, it is characterised in that in step (3), the method for the concentration is decompression
Concentration, it is preferred that pressure concentrated under reduced pressure is -0.1~-0.08MPa, temperature when being concentrated under reduced pressure is 38~47 DEG C.
10. the fruit aroma extract that the preparation method as described in claim 1~9 any claim is prepared is in cigarette
In application, it is preferred that the fruit aroma extract makes an addition at least one in pipe tobacco, cigarette paper, filter stick；More preferably
, the mass percentage content when fruit aroma extract is made an addition in pipe tobacco is 0.01%~0.5%, and the fruit is fragrant
Mass percentage content when taste extract makes an addition to cigarette paper is 0.05%~1.0%, the fruit aroma extract addition
Mass percentage content when filter stick is 0.02%~2%.
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| CN107881011A (en) * | 2017-12-12 | 2018-04-06 | 江西中烟工业有限责任公司 | A kind of preparation method of compound fruit medicinal extract |
| CN108077997A (en) * | 2017-12-12 | 2018-05-29 | 江西中烟工业有限责任公司 | A kind of preparation method and applications of cigarette filter combination |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101720957A (en) * | 2008-10-21 | 2010-06-09 | 北京市农林科学院 | Purple corn beverage and preparation method thereof |
| CN101768515A (en) * | 2010-01-13 | 2010-07-07 | 周煜航 | Preparation method and application of natural extractive |
-
2016
- 2016-12-29 CN CN201611241263.7A patent/CN106753796A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101720957A (en) * | 2008-10-21 | 2010-06-09 | 北京市农林科学院 | Purple corn beverage and preparation method thereof |
| CN101768515A (en) * | 2010-01-13 | 2010-07-07 | 周煜航 | Preparation method and application of natural extractive |
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| CN107881011A (en) * | 2017-12-12 | 2018-04-06 | 江西中烟工业有限责任公司 | A kind of preparation method of compound fruit medicinal extract |
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| CN107881011B (en) * | 2017-12-12 | 2021-02-26 | 江西中烟工业有限责任公司 | Preparation method of composite fruit extract |
| CN108142825A (en) * | 2017-12-22 | 2018-06-12 | 广西兴业百谷米业有限公司 | A kind of dietary fiber rice of high-content and preparation method thereof |
| CN108378412A (en) * | 2018-02-26 | 2018-08-10 | 陕西杨凌陕特农业发展有限公司 | A kind of tobacco processing procedure containing honey |
| CN109105858A (en) * | 2018-07-18 | 2019-01-01 | 中国热带农业科学院农产品加工研究所 | A kind of pineapple bonding state flavouring agent extracts and fragrance release process and its application |
| CN109123664A (en) * | 2018-07-18 | 2019-01-04 | 中国热带农业科学院农产品加工研究所 | A kind of passionflower bonding state flavouring agent extracts and fragrance release process and its application |
| CN109730366A (en) * | 2019-03-05 | 2019-05-10 | 铂德（深圳）科技有限公司 | Cherry extract solution, cherry electronics tobacco tar and preparation method thereof |
| CN114449904A (en) * | 2019-09-27 | 2022-05-06 | Cj第一制糖株式会社 | Evaporation module for producing a concentrate and liquid product manufacturing system comprising such an evaporation module |
| CN113712238A (en) * | 2021-09-03 | 2021-11-30 | 福建中烟工业有限责任公司 | Preparation of coral algae enzymolysis product and application of coral algae enzymolysis product in cigarettes |
| CN113712238B (en) * | 2021-09-03 | 2023-03-10 | 福建中烟工业有限责任公司 | Preparation of coral algae enzymolysis product and application of coral algae enzymolysis product in cigarettes |
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Application publication date: 20170531 |