CN101766657B - Compound for activating immune cell and preparation method thereof - Google Patents
Compound for activating immune cell and preparation method thereof Download PDFInfo
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- CN101766657B CN101766657B CN2008101877443A CN200810187744A CN101766657B CN 101766657 B CN101766657 B CN 101766657B CN 2008101877443 A CN2008101877443 A CN 2008101877443A CN 200810187744 A CN200810187744 A CN 200810187744A CN 101766657 B CN101766657 B CN 101766657B
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Abstract
The invention relates to a compound for activating immune cell and a preparation method thereof. Particularly, the invention provides a compound for activating immune cell activity in animal body, which comprises mushroom component, beneficial bacterium component and excipient. The invention also provides a preparation method of the compound, which comprises: taking mushroom mycelium for liquid culture to obtain mushroom component, culturing beneficial bacterium in appropriate culture medium to obtain beneficial bacterium component, and mixing the obtained mushroom component, the beneficial bacterium component and the excipient to obtain the compound.
Description
Technical field
The present invention relates to activate the composition and method of making the same of immunologic cellular activity in the animal body.
Background technology
There are in recent years many researchs to find to contain considerable physiologically active ingredient in mushroom mycelium or the sporophore, comprise polysaccharide body, triterpenes, protein, nucleic acid, steroid etc., mentioned component has the effects such as antitumor, enhancing immunity, reduction hypertension, blood sugar lowering, inhibition platelet aggregation, antiviral.
Known polysaccharide body has the antineoplastic effect, and its physiologically active can roughly be divided into three classes according to the molecular weight of polysaccharide body: (A) molecular weight is 3,000～5, about 000, have the function of blood sugar lowering, (B) molecular weight is 10,000～100, between 000, the effect of tool antiinflammatory, (C) molecular weight is approximately more than 30,000, then have antitumor action, and the larger effectiveness of molecular weight is better.
Fungus polysaccharide extracted mostly from sporophore in the past, but the sporophore source is limited; Recently research is known approximately to have 50% mushroom class to produce the extracellular polysaccharide body in liquid state is cultivated, so desirable its culture fluid carries out polysaccharide body purification; Cultivate liquid the cultivation comparatively rapidly and simply than sporophore.
The product of the present extract of pestering on the market is numerous, particularly contains the health food of the extract of pestering.Therefore, the related needs such as the prescription of increase mushroom extract nutritive value, the new application of mushroom extract or new product still have its necessity.
In the field of health food, probiotic bacteria also is one of research emphasis.The bacterium phase that distributes in the intestinal of human body has the antibacterial that surpasses more than 100 kinds to exist according to estimates, wherein is made of 99% bacterium amount 30～40 kinds of antibacterials, and anaerobe is preponderated approximately many 100 to 10,000 times than aerobic bacteria.The digestive tract zones of different exists different strains and different distributed number.These intestinal floras form the relation of symbiosis, antagonism each other, and keep the balance of flora ecology in the individuality of health.Wherein, the small part antibacterial is harmful, for example: bacillus perfringens and staphylococcus aureus, can wait for an opportunity hypertrophy because host immune power descends, cause disease; Yet, the antibacterial that inhabits the healthy human body intestinal is large absolutely a plurality of to the human body beneficial, be commonly referred to as probiotics (beneficialbacteria), wherein be widely known by the people most with lactobacillus (Lactic acid bacteria) or bacillus bifidus (Bifidobacterium).
Existing many research reports are pointed out, probiotics has the multiple function that promotes health, comprise: (1) keeps the normal microbial of intestinal: the acetic acid and the lactic acid that produce by the probiotics metabolism, make intestinal effectively maintain lower pH value, make pathogenicity and corruptibility antibacterial be difficult for growth; (2) activated immune system: probiotics has stimulating immune system, increases the activity of antibody or macrophage, its cell wall contains some antigenic component, can bring out host immune system activation, suppress tumor, or make the host produce antibody to be adsorbed on the intestinal mucosa surface to suppress pathogen; (3) improve nutritive value: take bacillus bifidus as example, it has acidization and proteolysis is active, can change nitrogen-containing compound composition in the food and improve protein digestibility, and can promote the absorption of calcium and other nutrition, and then prevention of osteoporosis disease, even some bacterial strain can synthesize multivitamin and folic acid; (4) promote lactic acid metabolism; (5) improve the diarrhoea constipation: the organic acid that probiotics produces can be promoted intestinal peristalsis promoting, improves the moisture in the feces, to reduce the intestinal retardance; (6) reduce blood cholesterol: although this function is still disputed, but the result learns by the part in vitro tests, some probiotics can make cholesterol discharge human body with going the co-precipitation of conjunction type cholate with feces, also has animal test results to show, edible lactobacillus can reduce the concentration of blood cholesterol; (7) suppress hypertension: some metabolite of probiotics can suppress the angiotonin conversion and make Blood pressure drop.
Scientist studies the mechanism of action of probiotics and finds probiotics meeting production probiotics prebiotics (Prebiotics), and promotion or the maintenance of health had the best effect.
Therefore, the present invention is according to above-mentioned concept and in response to the market demand, and the new compositions of health-care effect is provided in exploitation.
Summary of the invention
One object of the present invention is providing a kind of compositions that activates immunologic cellular activity in the animal body.
For reaching above-mentioned and other purpose, compositions of the present invention comprises: mushroom constituent, probiotics composition and excipient.
According to the present invention, mushroom constituent comprises mushroom mycelium.The mushroom constituent of the present composition comprises fungus polysaccharide, particularly beta glucan.Mushroom constituent of the present invention also can comprise the mushroom metabolite except fungus polysaccharide, such as: the materials such as protein, polypeptide class, triterpenes, several amino acids, alkaloid, esters and organic acid.Compositions of the present invention can comprise the mixture of a kind of mushroom constituent or multiple mushroom constituent.
According to the present invention, described mushroom is selected from Schizophyllum commune Franch． (Schizophyllum commue); Brazilian mushroom (Agarics blaze); Cordyceps (Cordyceps sinensis); Ganoderma (Ganoderma lucidum); Coriolous Dersicolor (Fr.) Quel (Coriolus versicolor); Antrodia camphorata (Anthodiacamphorate); Phellinus igniarius (L. ex Fr.) Quel. (Phellinus linteus); Mushroom Corals (Pleurotuscitrinopileatus); Lentinus Edodes (Lentinula edodes); Liu Songgu (Agrocybe aegerita); Hericium erinaceus (Bull. Ex Fr.) Pers. (Hericium erinaceus); Pleurotus eryngii (Pleurotus eryngiig); petal fine and soft (Sparrasis crispa); Auricularia (Auricularia auricula); the combination of JINZHENGU (Flammulinavelutipes) or above-mentioned mushroom.
According to the present invention, the probiotics composition comprises the probiotics thalline, and this probiotics is selected from lactobacillus acidophilus (Lactobacillus acidophilus), bacillus bifidus (Bifidobacteriumlongum), Lactobacillus paracasei (Lactobacillus paracasei), enterococcus (Enterococcusfaecium) or its combination.
According to the present invention, the probiotics composition comprises the probiotics prebiotics that the probiotics strain produces in incubation.
According to the present invention, the probiotics composition is probiotics thalline, probiotics prebiotics or its mixture.
According to the present invention, excipient is, for example and unrestricted, and Semen Coicis, lactose, white sugar, PEARLITOL 25C, starch, corn starch, cellulose crystals or anhydrous silicic acid; In a preferred embodiment, excipient is lactose; In another preferred embodiment, excipient is Semen Coicis.
Compositions of the present invention further comprises additive, and this additive can be pharmaceutically, on the food or the upper acceptable additive of beauty treatment.
In an embodiment of the present invention, additive is food; In a preferred embodiment, additive is selected from rice, wheat and barley, broomcorn millet class, beans, Semen Sesami, Semen Ricini or its mixture; In more preferred embodiment, additive is brown rice, Semen Maydis or both mixture.
Another object of the present invention provides the compositions that comprises one or more mushroom constituents, one or more probiotics compositions and excipient.In a preferred embodiment, mushroom is selected from Schizophyllum commune Franch．, Brazilian mushroom, Cordyceps, Ganoderma, Coriolous Dersicolor (Fr.) Quel, Antrodia camphorata, Phellinus igniarius (L. ex Fr.) Quel., Mushroom Corals, Lentinus Edodes, Liu Songgu, Hericium erinaceus (Bull. Ex Fr.) Pers., Pleurotus eryngii, petal young pilose antler, Auricularia, JINZHENGU or its combination; Probiotics is selected from lactobacillus acidophilus, bacillus bifidus, Lactobacillus paracasei, enterococcus or its combination; And this excipient is lactose or Semen Coicis.
According to the present invention, compositions can be for directly, or optionally mixed carrier, medicine, food, cosmetics or other compositions for follow-up use.
Another object of the present invention provides the compositions that comprises one or more mushroom constituents, one or more probiotics compositions and additive.In a preferred embodiment, mushroom is selected from Schizophyllum commune Franch．, Brazilian mushroom, Cordyceps, Ganoderma, Coriolous Dersicolor (Fr.) Quel, Antrodia camphorata, Phellinus igniarius (L. ex Fr.) Quel., Mushroom Corals, Lentinus Edodes, Liu Songgu, Hericium erinaceus (Bull. Ex Fr.) Pers., Pleurotus eryngii, petal young pilose antler, Auricularia, JINZHENGU or its combination; Probiotics is selected from lactobacillus acidophilus, bacillus bifidus, Lactobacillus paracasei, enterococcus or its combination; Additive is selected from rice, wheat and barley, broomcorn millet class, beans, Semen Sesami, Semen Ricini or its mixture.In more preferred embodiment, additive is brown rice, Semen Maydis or both mixture.
According to the present invention, compositions can be for directly, or optionally mixed carrier, medicine, food, cosmetics or other compositions for follow-up use.
Another object of the present invention provides a kind of method for preparing the compositions of immunologic cellular activity in the activation animal body, comprises the following steps: to get mushroom mycelium and carries out the liquid state cultivation, to obtain mushroom constituent; Cultivate probiotics with appropriate culture medium, to obtain the probiotics composition; And mix mushroom constituent, probiotics composition and excipient, to obtain compositions.
According to the present invention, mushroom is selected from Schizophyllum commune Franch．, Brazilian mushroom, Cordyceps, Ganoderma, Coriolous Dersicolor (Fr.) Quel, Antrodia camphorata, Phellinus igniarius (L. ex Fr.) Quel., Mushroom Corals, Lentinus Edodes, Liu Songgu, Hericium erinaceus (Bull. Ex Fr.) Pers., Pleurotus eryngii, petal young pilose antler, Auricularia, JINZHENGU or its combination.
According to the present invention, the liquid mushroom constituent of cultivating gained comprises mushroom mycelium and/or fungus polysaccharide.
According to the present invention, probiotics is selected from lactobacillus acidophilus, bacillus bifidus, Lactobacillus paracasei, enterococcus or its combination.
According to the present invention, the probiotics composition that obtains through appropriate culture medium comprises probiotics thalline and/or probiotics prebiotics.
According to the present invention, excipient is Semen Coicis, lactose, white sugar, PEARLITOL 25C, starch, corn starch, cellulose crystals or anhydrous silicic acid; In a preferred embodiment, excipient is lactose; In another preferred embodiment, this excipient is Semen Coicis.
Method of the present invention further comprises and adds additive to form compositions, and wherein additive is selected from rice, wheat and barley, broomcorn millet class, beans, Semen Sesami, Semen Ricini or its mixture.
Description of drawings
Fig. 1 is mushroom of the present invention and probiotics compositions preparation flow.
Fig. 2 is bar chart, and it is according to embodiments of the invention, shows that mushroom of the present invention and probiotics compositions are for the impact of natural killer cell activity in the rat body; Wherein Control is that matched group, A and B are that feeding probiotic group, C and D are feeding present composition group.
The specific embodiment
Below be that those skilled in the art can understand other advantages of the present invention and effect by content disclosed in the present specification by particular specific embodiment explanation embodiments of the present invention.
1. mushroom is cultivated
The spendable mushroom mycopremna of the present invention such as Schizophyllum commune Franch．, Brazilian mushroom, Cordyceps, Ganoderma, Coriolous Dersicolor (Fr.) Quel, Antrodia camphorata, Phellinus igniarius (L. ex Fr.) Quel., Mushroom Corals, Lentinus Edodes, Agrocybe such as Liu Songgu, Hericium erinaceus (Bull. Ex Fr.) Pers., Pleurotus eryngii, petal young pilose antler, Auricularia, JINZHENGU etc., but be not limited to above-mentioned bacterial strains.Select one to cultivate.
With bacterial classification inoculation on YM agar culture medium (the YM agar culture medium comprises 0.3% (w/w) yeast extract, 0.3% Fructus Hordei Germinatus extract, 0.5% peptone, 1.0% dextrose, 1.5% agar etc.), place 28 ℃ of cultivations, wherein different according to strain, mycelium covered with the required incubation time of solid medium approximately 7 days～20 days; For example: Schizophyllum commune Franch． is approximately cultivated rear 2-3 days visible mycelium, approximately can cover with solid medium in 5-7 days.
Above-mentioned solid-state cultured mycelia is cut a fritter implant 50 milliliters of YM culture fluid, in the T-shaped animal cell culture box of 80T, cultivate a week at 28 ℃, as the strain of the preposition fermentation of liquid state.
In the wide-mouth serum bottle, prepare 800 milliliters YM culture fluid, and sterilize after putting into stirrer, to cultivate the mushroom bacteria liquid of finishing at T-shaped plastic culture box is inoculated in the serum bottle, inoculative proportion is that per 10 milliliters of fresh mediums are inoculated into 1 milliliter of bacterium liquid, then serum bottle is placed on the Magnetitum blender (rotating speed 300rpm), under room temperature, stir, to carry out the preposition fermentation first time, cultivate after 72 to 96 hours, the extracellular polysaccharide bulk concentration reaches the highest.
With same steps as, in 10 liters of serum bottle preparation approximately 8 liters of culture fluid (contain 10 gram peptones, 10 gram beef extracts, 5 gram yeast extracts, 20 gram dextroses, 1 gram Tween 80,2 gram ammonium citrates, 5 gram CH in 1 liter of culture fluid
3COONa, 0.1 gram MgSO
4C.7H
2O, 0.05 gram MnSO
4C.H
2O and 2 gram K
2HPO
4And with pH-value furnishing pH 6.2-6.5), the bacterium liquid that for the first time preposition fermentation of inoculation is finished carries out the preposition fermentation second time.
Certainly, get the preposition fermentation of optionally carrying out repeatedly.Then, the fermentation liquid of preposition fermentation being finished carries out bulk fermentation.
In 20 liters of polycarbonate resin (Polycarbonate, PC) the productive culture base (comprising 4% glucose and 0.5% yeast extract) of 13 liters of optimizations of preparation in the culture vessel, after stirring, cover silica gel plug, with autoclave sterilization, wherein sterilising conditions is 121 ℃, 15 minutes, cools to room temperature.Then, the 2-3 litre bacterium liquid that preposition fermentation is finished the above-mentioned second time is inoculated in the polycarbonate resin culture vessel, be positioned on the wobbler, the setting rotating speed is 80rpm, under room temperature (approximately 25 ℃), ferment to the resting stage (stationaryphase) of growth curve, mycelial cell number and the polysaccharide bulk concentration in this period are kept homeostasis, namely stop fermentation.Gained is cultivated liquid and is comprised the materials such as fungus polysaccharide, protein, polypeptide class, triterpenes, several amino acids, alkaloid, esters and organic acid.
2. probiotics cultivation and probiotics metabolite preparation
Carry out the probiotics spawn culture, wherein this probiotics is lactobacillus acidophilus, bacillus bifidus, Lactobacillus paracasei, or enterococcus.
Bacterial classification inoculation (is contained 10 gram peptones, 10 gram beef extracts, 5 gram yeast extracts, 20 gram dextroses, 1 gram Tween 80,2 gram ammonium citrates, 5 gram CH in the planar solid culture medium in 1 liter of culture fluid
3COONa, 0.1 gram MgSO
4C.7H
2O, 0.05 gram MnSO
4C.H2O, 2 gram K
2HPO
4With 15 gram agar, and with pH-value furnishing pH 6.2-6.5), place 37 ℃ of cultivations.Different according to strain, can on culture medium, grow bacterium colony in approximately 1 day～3 days.
Choose single bacterium colony and carry out the liquid cultivation of a small amount of, then cultivate in a large number, utilize different existing grown cultures based components can obtain different probiotics metabolite and probiotics prebiotics.Culture fluid can be directly used in subsequent group compound preparation, also thalline and culture fluid can be separated with centrifugation, and only with thalline for follow-up.
3. the preparation of the present composition
(embodiment 1)
Will be by the mushroom fermentation liquid of said method gained with after the probiotics culture fluid mixes, utilize high-shear homogenizer processed 10 minutes with 12,000rpm and homogeneous solution.Then add the lactose excipient, wherein the ratio of homogeneous solution and excipient is 1: 1, behind the mix homogeneously, after freezing, process with low-temperature vacuum drying (lyophilization), wherein the operating condition of low-temperature vacuum drying is-40 ℃, 0.3MPa, uses mixture is concentrated into to form moisture content and be lower than 5% powder.
As required lactose is replaced with other excipient, for example: Semen Coicis, white sugar, PEARLITOL 25C, starch, corn starch, cellulose crystals or anhydrous silicic acid.
(embodiment 2)
To be mixed with the probiotics culture fluid by the mushroom fermentation liquid of said method gained, via high-shear homogenizer with 12,000rpm processed 10 minutes and got homogeneous solution, added the brown rice granule (fermentation liquid: the ratio of brown rice is 2: 1) that grinds in advance again, carried out lyophilization after evenly mixing and processed.Under 10 ℃, utilize high-shear homogenizer to clay into power in the mixture after the lyophilization, to obtain to contain the composition powder of mushroom constituent and probiotics composition.
As required brown rice is replaced with other frumentums, for example: rice, wheat and barley, broomcorn millet class, beans, Semen Sesami, Semen Ricini and composition thereof.
4. the present composition is used for the effect of activating immune cell activity
Zootype:
Select five the week ages male Wistar whitewash mouse (source: Taiwan Univ.'s animal center), weight be 380～450 the gram.Before the experiment rat is raised separately respectively, room temperature is controlled in 21 ± 2 ℃, humidity 65 ± 5%, and the dark circulation time of light is 12D:12L, begins to test after adapting to fortnight.
With present composition feeding rat three days, 0.2 milliliter of every day.Use LIVE/DEAD Cell-Mediated Cytotoxicity test kit (Molecular Probes) to measure natural killer cell activity in the blood after three days, experimental procedure is as follows:
With YAC-1 cell concentration furnishing 1 * 10
6Cells/ml.Add to scale DIOC
18(10 μ LDIOC
18/ milliliter YAC-1 cell suspending liquid) also evenly mixed.Cultivated 20 minutes in 37 ℃.With cell concentration furnishing 1 * 10
6The cell/ milliliter.
With RPMI 1640 culture medium (Hyclone) with lymphocytes furnishing cell concentration 2 * 10
6The cell/ milliliter.In spleen lymph corpuscle suspension 200 microlitres that YAC-1 cell 20 microlitres after the dyeing are added, and add PI solution (40 mcg/ml) dyeing of 220 microlitres,, cultivated 2 hours in 37 ℃ again after centrifugal 30 seconds with 1000 * g.Measure lymph corpuscle with flow cytometer active.
The result compares with matched group as shown in Fig. 2, and the present composition can promote the activity of natural killer cell really.
But compositions of the present invention has more advantage and the effect of the following stated except the activating immune cell activity:
Compositions of the present invention also has other health-care effecies, grows, supplements the nutrients, adsorbs noxious substance, improves skin quality etc. as suppressing noxious bacteria, therefore can promote health, the infringement of minimizing disease of animal body or human body.
The probiotics prebiotics that compositions of the present invention comprises comprises organic acid (such as lactic acid or acetic acid) and antimicrobial factors, can suppress the growth to animal body or human body harmful antibacterial.
Compositions of the present invention can provide the abundant nutrition composition, being mainly mushroom and probiotics generates in incubation, such as: fungus polysaccharide, protein, polypeptide class, triterpenes, several amino acids, alkaloid, esters, organic acid, vitamin B group and ferment etc., can be added into food or feedstuff, for the nutrition of animal body or needed by human body, use as adding.
In addition, compositions of the present invention can neutralize and absorbing toxin, therefore can lower human body the absorption of nitramine and phenol is reduced carcinogenic risk.But compositions of the present invention also the cholesterol reducing absorbance, strengthen the application of mono-and di-saccharides and reduce its body internal absorption factor, can be applicable to diet control and the nutritional supplementation of diabetics.
Compositions water soluble of the present invention is got its aqueous solution and is applied in skin, can be absorbed by the skin rapidly, and the immunocyte in the activation skin histology (such as the fibroblast of phagocyte and manufacturing collagen protein), can improve skin quality and prevent dermatosis.
Above-described embodiment is illustrative compositions of the present invention and preparation method only, but not is used for restriction the present invention.Any those skilled in the art all can be under spirit of the present invention and category, and above-described embodiment is modified and changed.Therefore, the scope of the present invention as claimed in claim.
Claims (18)
1. compositions that activates immunologic cellular activity in the animal body comprises:
Mushroom constituent;
The probiotics composition; And
Excipient, wherein said compositions are that the homogeneous solution of being mixed with the probiotics culture fluid that contains this probiotics composition by the mushroom fermentation liquid that contains this mushroom constituent and the ratio of excipient are 1: 1 prepared person.
2. compositions according to claim 1, wherein mushroom constituent comprises mushroom mycelium.
3. compositions according to claim 1, wherein mushroom constituent comprises fungus polysaccharide and/or mushroom metabolite.
4. compositions according to claim 1, wherein the probiotics composition comprises the probiotics thalline.
5. compositions according to claim 1, wherein the probiotics composition comprises the probiotics prebiotics.
6. compositions according to claim 1, wherein mushroom is selected from the combination of Schizophyllum commune Franch．, Brazilian mushroom, Cordyceps, Ganoderma, Coriolous Dersicolor (Fr.) Quel, Antrodia camphorata, Phellinus igniarius (L. ex Fr.) Quel., Mushroom Corals, Lentinus Edodes, Liu Songgu, Hericium erinaceus (Bull. Ex Fr.) Pers., Pleurotus eryngii, petal young pilose antler, Auricularia, JINZHENGU or above-mentioned mushroom.
7. compositions according to claim 1, wherein probiotics is selected from lactobacillus acidophilus, bacillus bifidus, Lactobacillus paracasei, enterococcus or its combination.
8. compositions according to claim 1, wherein excipient is lactose, Semen Coicis, white sugar, PEARLITOL 25C, starch, corn starch, cellulose crystals or anhydrous silicic acid.
9. compositions according to claim 1 further comprises frumentum.
10. compositions according to claim 8, wherein frumentum is selected from rice, wheat and barley, broomcorn millet class, beans, Semen Sesami, Semen Ricini or its mixture.
11. a method for preparing the compositions of immunologic cellular activity in the activation animal body comprises the following steps:
Get mushroom mycelium and carry out the liquid state cultivation, to obtain to contain the mushroom fermentation liquid of mushroom constituent;
Cultivate probiotics with appropriate culture medium, to obtain to contain the probiotics culture fluid of probiotics composition; And
Mixed as 1: 1 with the ratio of excipient take the homogeneous solution of being mixed with this probiotics culture fluid by this mushroom fermentation liquid, to obtain compositions.
12. method according to claim 11; wherein, mushroom is selected from the combination of Schizophyllum commune Franch．, Brazilian mushroom, Cordyceps, Ganoderma, Coriolous Dersicolor (Fr.) Quel, Antrodia camphorata, Phellinus igniarius (L. ex Fr.) Quel., Mushroom Corals, Lentinus Edodes, Liu Songgu, Hericium erinaceus (Bull. Ex Fr.) Pers., Pleurotus eryngii, petal young pilose antler, Auricularia, JINZHENGU or above-mentioned mushroom.
13. method according to claim 11, wherein, probiotics is selected from lactobacillus acidophilus, bacillus bifidus, Lactobacillus paracasei, enterococcus or above-mentioned combination.
14. method according to claim 11, wherein, excipient is lactose or Semen Coicis.
15. method according to claim 11 further comprises the adding frumentum.
16. method according to claim 15, wherein, frumentum is selected from rice, wheat and barley, broomcorn millet class, beans, Semen Sesami, Semen Ricini or its mixture.
17. method according to claim 11, wherein mushroom constituent comprises mushroom mycelium and/or fungus polysaccharide.
18. method according to claim 11, wherein the probiotics composition comprises probiotics thalline and/or probiotics prebiotics.
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| CN102485234A (en) * | 2010-12-06 | 2012-06-06 | 乔本生医股份有限公司 | Composition used for preventing and/or treating liver damage caused by acute hepatitis |
| CN103027196A (en) * | 2013-01-18 | 2013-04-10 | 吉林农业大学 | Feed additive |
| CN104187554B (en) * | 2014-08-08 | 2015-11-25 | 江苏神华药业有限公司 | A kind of nutrition health care condiment containing phellinus igniarius mycelium powder and preparation method thereof |
| CN108077933A (en) * | 2017-12-20 | 2018-05-29 | 江苏天美健大自然生物工程有限公司 | A kind of health products of the auxiliary protection chemical damage containing Liu Songgu |
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Effective date of registration: 20220805 Address after: 109 Holland Road 5, Irvine, California, USA Patentee after: Meishang Tangrui Co., Ltd. Address before: Taiwan, Taipei, China Patentee before: Chen Xiunan |