CN101732669B - Traditional Chinese medicine used for treating gynecologic inflammation, preparation method and application thereof - Google Patents
Traditional Chinese medicine used for treating gynecologic inflammation, preparation method and application thereof Download PDFInfo
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Abstract
The invention provides a traditional Chinese medicine composition aiming at the technical defect. By reducing fever, eliminating dampness, decreasing swelling, lessening the virulence of any pathogenic organism, relieving the stasis of blood, eliminating carbuncles, promoting the circulation of qi and alleviating pain, the aim of treating gynecologic inflammation is achieved. In order to achieve this aim, the invention adopts the following technical scheme: the active components contained in a traditional Chinese medicine used for treating the gynecologic inflammation are prepared from the following raw materials in parts by weight: 10-50 parts of herba patriniae, 10-50 parts of sargentodoxa cuneata, 5-25 parts of rhizoma zedoariae and 5-25 parts of herba artemisiae. The traditional Chinese medicine preparation has the efficacies of reducing fever, eliminating dampness, decreasing swelling, lessening the virulence of any pathogenic organism, relieving the stasis of blood, eliminating carbuncles, promoting the circulation of qi and alleviating pain.
Description
Technical field
The present invention relates to the Chinese medicine preparation technical field, be specifically related to a kind of Chinese medicine and method for making and application that is used to treat gynecological inflammation.
Background technology
Gynecological inflammation is meant that female genital system infects caused disease, is adult female's common frdquently encountered disease, the adult female in the whole world about 87% infection gynecological inflammation in various degree.The gynecological inflammation clinical manifestation is also varied, and the cause of disease is complicated and often with multiple severe complication.Common clinically have vaginitis, cervicitis, pelvic inflammatory disease, an endometritis etc., often prolongedly do not heal.Gynecological inflammation has become puzzlement China women's a big disease, and prevalence is high, and the relapse rate height is the main feature of gynaecological inflammation disease, reflects that simultaneously the demand in gynecological inflammation medicine market is huge.Chinese patent medicine occupies leading position in gynecological inflammation medication market, because the Chinese patent medicine side effect is little, determined curative effect is applicable to long-term prescription, has adapted to the characteristics of gynecological inflammation easy relapse, and clinical practice is very extensive.
Though there is the Chinese patent medicine of a lot of treatment gynecological inflammations in market; But most of curative effects are undesirable, and these medicines are many because of there are defective in prescription and preparation technology, in light of this situation; According to the gynecological inflammation etiology and pathogenesis; Establish rational prescription, improve preparation technology, become urgent problem to improve the content of effective ingredient.Like employing depressed liver-energy dispersing and QI regulating blood-activating analgetic method (square medicine is formed: Rhizoma Cyperi, Radix Bupleuri, Fructus Toosendan, Rhizoma Corydalis, Radix Angelicae Sinensis, Rhizoma Chuanxiong, Radix Paeoniae Rubra, the Radix Paeoniae Alba, Radix Salviae Miltiorrhizae, Semen Persicae, Fructus Liquidambaris, Poria, Radix Glycyrrhizae etc.) treatment chronic pelvic inflammatory diseases such as Wang Shi, (Wang Meifang etc.Chronic pelvic inflammatory disease 48 examples are treated in the ruling by law of depressed liver-energy dispersing and QI regulating blood-activating analgetic; Shandong journal of Chinese medicine; 1997; 16 (7) 306-307), Young adopts regulating qi to disperse stagnation method (square medicine is formed: Radix Angelicae Sinensis, Flos Carthami, Radix Salviae Miltiorrhizae, triangular, Rhizoma Curcumae, Fructus Aurantii, Rhizoma Cyperi (processed), Rhizoma Corydalis, Rhizoma Alismatis, Ramulus Cinnamomi, Caulis Sargentodoxae, Herba Patriniae) treatment chronic pelvic inflammatory disease (the Jiangsu traditional Chinese medical science 1997,18 (8) 18) all can not obtain good result.
This subject study; Can actively prevent and treat the generation and the development of gynecological inflammation effectively, and can prevent canceration, this for the existence that improves numerous women and quality of life, protect numerous women's health; And then the raising productivity, significant and higher utility.Because the primary disease sickness rate is high, estimate to use once marketing, will produce good social benefit and economic benefit.
We derive from inventor's gynecological clinic experience of three more than ten years, and the prescription of being correlated with is at all times being carried out on the basis of system's further investigation, form through long-term clinical optimization screening.
Summary of the invention
Goal of the invention: the present invention is directed to above-mentioned technological deficiency, a kind of Chinese medicine composition is provided, through clearing heat and expelling damp; Subduing swelling and detoxicating, the blood stasis dispelling eliminating carbuncle, promoting the circulation of QI to relieve pain and reach the treatment gynecological inflammation purpose; In order to reach this order ground, adopt following technical scheme:
Technical scheme: a kind of Chinese medicine that is used to treat gynecological inflammation, its contained active component is prepared from the following raw materials in part by weight proportioning: Herba Patriniae 10-50 part, Caulis Sargentodoxae 10-50 part, Rhizoma Curcumae 5-25 part, Herba Artemisiae Anomalae 5-25 part.
A method for the treatment of gynecological inflammation medicine, it contains the active ingredient by the following weight ratio of raw materials prepared from: Patrina 10-50 copies cuneata 10-50 parts, Curcuma 5-25 parts per HU 5-25, 4-18 to Bie insect parts, TPG 10-50 parts, Dan 5-25, 5-25 Siphonostegia.
A method for the treatment of gynecological inflammation medicine, it contains the active ingredient by the following weight ratio of raw materials prepared from: Patrinia 30 copies, 30 copies cuneata, Curcuma 15 copies, 15 copies Yuan Hu, ground Bie 10 parts of insects, red peony 30 parts, Dan 15 parts, 15 parts of Salvia.
The above-mentioned Chinese medicine that is used to treat gynecological inflammation, dosage form are granule, tablet, oral liquid, capsule, washing liquid or suppository.
Preparation is used to treat the Chinese medicine preparation of gynecological inflammation, and method for preparing is following:
A. get the recipe quantity of Rhizoma Corydalis, put into 90%wt, each reflux, extract, 1h of 70%wt ethanol of being respectively 8～10 times of prescription weight successively, filter, merging filtrate reclaims ethanol, and medicinal residues are subsequent use;
B. get the recipe quantity of Cortex Moutan, add the water of 10～12 times of weight of prescription, soak 2～3h, adopting altogether, hydrodistillation extracts paeonol; Collect 9 times of amounts of paeonol and Cortex Moutan medical material amount saturated aqueous solution, and add the NaCl of 3% Cortex Moutan medical material amount, cold preservation 24h, sucking filtration to it; Get paeonol, the device preservation is subsequent use in addition for medicinal residues, utilizes ultrasonic method to prepare the paeonol clathrate, behind 24 times of paeonol weight dissolve with ethanol paeonol; Add 8～10 times of amount beta-schardinger dextrin-s (β-CD), supersound process 40～60min, cold preservation 24h; Sucking filtration, filtering residue are used the petroleum ether after drying, and be subsequent use;
C. get the recipe quantity of Rhizoma Curcumae, add the water of 8～10 times of prescription weight, soak 0.5～1h, the utilization steam distillation extracts volatile oil 5～6h, leaves standstill 24h, cold preservation, and it is subsequent use to keep medicinal residues;
d. taken cuneata, ground Bie insects, Patrinia, TPG, Siphonostegia prescription volume, and a, b, c Step spare dregs, add boiling water three times, respectively, followed by adding the total weight of herbs 10 times, 8 times, 8 times the amount of water, each refluxed 1h, concentrated extract to a relative density of 1.10 to 1.25, plus 60% wt ~ 70% wt of ethanol and allowed to stand refrigerated for more than 24h, filtered, the recovery of ethanol, the filtrate and the The combined solution was concentrated to continue the relative density of 1.05 to 1.20 of the extract, dried and crushed into a dry extract powder;
E. with the dry extract of d step gained and the paeonol beta-CD inclusion of above-mentioned b step gained, c step gained Rhizoma Curcumae volatile oil uniform mixing adds adjuvant again, processes required preparation.
The application of described Chinese medicine in preparation treatment gynecological inflammation medicine.
Beneficial effect: existing through looking into new qualification result prompting: aspect the rule of treatment, seeing has depressed liver-energy dispersing and QI regulating, blood-activating analgetic, regulating qi to disperse stagnation, blood circulation promoting and blood stasis dispelling; Hard masses softening and resolving; Heat-clearing and toxic substances removing etc. are not seen the report that " clearing heat and expelling damp, subduing swelling and detoxicating; blood stasis dispelling eliminating carbuncle, promoting the circulation of QI to relieve pain " therapeutic alliance gynecological inflammation is arranged, and used side's medicine also with detect document in used side's medicine different or incomplete same.To the pharmacodynamic study of treatment gynecological inflammation, see that having with inflammation, microcirculation, the threshold of pain, uterine smooth muscle contraction and hemorheology of rat is that index is studied, but drug prescription is incomplete same with this problem.
See from theory of Chinese medical science, Herba Patriniae among the we, bitter cold has heat-clearing and toxic substances removing, promoting blood circulation and breaking stagnation, the detumescence and apocenosis effect is controlled the acute appendicitis dysentery, leucorrhea with red and white discharge, the stasis of blood stagnated and suffered from abdominal pain effects such as carbuncle scabies puerperal." not Lu ": " remove carbuncle, edema, stagnation of pathogenic heat, migratory arthralgia is not enough, puerperal disease "." property of medicine opinion ": " control malicious wind insensitive impediment, main broken blood stasis for many years, can suppurate is water ".Caulis Sargentodoxae, bitter flat nontoxic, go into liver, large intestine channel, have the eliminating carbuncle of relieving internal heat, promoting blood circulation to remove obstruction in the collateral, the effect of wind dispelling insecticide, " Bencao Tujing ": " attack blood, control clot ", " Shaanxi Chinese herbal medicine ": " anti-inflammation, dispersing swelling and dissipating binds, vital energy regualting and blood circulation-promoting, wind dispelling insecticide; Modern study finds that this medicine all has bacteriostasis for staphylococcus aureus, escherichia coli.Herba Patriniae, Caulis Sargentodoxae are monarch drug altogether, and Rhizoma Curcumae toil temperature is nontoxic, go into liver, spleen channel, have the circulation of qi promoting removing blood stasis, and the effect of removing food stagnancy pain relieving is controlled trusted subordinate's distending pain, lumps in the chest and abdomen, women's blood stasis amenorrhea." the article remittance is smart to be wanted ": " removing mass gathers "." the SHENGCAO property of medicine is wanted fully ": " applying the skin ulcer detumescence, eliminating stasis to stop pain ".Modern study find this medicine can antitumor, antiearly pregnancy, antiinflammatory, antibiotic, effect such as enzyme falls; Rhizoma Corydalis has blood-activating and qi-promoting, the analgesic effect; Eupolyphaga Seu Steleophaga, salty-cold slightly poisonous, go into Liver Channel, removing blood stasis is arranged, reuniting the fractured tendons and bones, the effect of the pain relieving of curing the wound cures mainly the long-pending piece of blood stasis amenorrhea ， mass in the abdomen." property of medicine opinion ": " Zhi Yueshui is obstructed, and broken stagnant blood gathers.", " book on Chinese herbal medicine is new again ": " consumer edema relieves internal heat." modern study finds that this liquid medicine extract can significant prolongation bleeding time and recalcification time; obvious anticoagulant rate; shorten erythrocyte electrophoretic time, WBV, plasma viscosity, celloglobulin content are not all had obvious influence, more than three medicines be ministerial drug altogether; The liver spleen channel is gone in the bitter cold of Radix Paeoniae Rubra, the pain relieving of ability clots absorbing, removing heat from blood detumescence.Control the stagnant amenorrhea of the stasis of blood, the hernia abdominal mass is gathered, stomachache hypochondriac pain, dysentery, carbuncle." the southern regions of the Yunnan Province book on Chinese herbal medicine ": " sending down the abnormal ascending QI, promoting the circulation of blood, removing blood stasis with potent drugs, the clot that looses ends stomachache, moves back heat in blood, attacks the carbuncle skin ulcer.", " not Lu ": " clear and coherent blood vessels, slow in, dispelling malignant blood, expelling extravasated blood, the gas that anhydrates, subduing inflammation." pharmacological research prompting its have effects such as antibiotic, analgesic, calmness, spasmolytic, antiinflammatory, antiulcer; Cortex Moutan is arduous cool, goes into the conscience kidney channel, clearing away heat and cooling blood and blood repercussive.Control the pathogenic heat attacking blood system in febrile diseases, the amenorrhea lump in the abdomen, carbuncle and ulcer is pounced on damage." the southern regions of the Yunnan Province book on Chinese herbal medicine " tool: " removing blood stasis, promoting the circulation of blood, the disease of the mass in the abdomen that disappears, the heat of dehematize branch." modern study finds that this medicine has effects such as antibiotic, antiinflammatory, analgesia, spasmolytic, and can reduce vascular permeability.The bitter temperature of Herba Artemisiae Anomalae is nontoxic, goes into the heart, spleen, liver, kidney channel, and removing blood stasis stimulates the menstrual flow, healing up sore and subduing swelling, hemostasis analgesic therapy.Arrogate to oneself and control amenorrhea and mass in the abdomen, chest and abdomen swelling and pain, puerperal blood stasis, traumatic injury, incised wound is hemorrhage, Yong Du burning swelling.The Tang Materia Medica cloud: main " main removing blood stasis expands down.", " Japan hanako materia medica " carries " controlling pain in the chest and abdomen, therapeutic method to keep the adverse qi flowing downward water swelling, vim and vigour, logical married woman's passages through which vital energy circulates crux ", the swollen carbuncle poison of " book on Chinese herbal medicine covers bamboo fish trap ": “ Xiao inflammation, the burning hot pain of the soup that goes out ".Be adjuvant drug altogether.The all medicines in full side are harmonious, and play clearing heat and expelling damp altogether, removing toxicity for eliminating carbuncles, regulating qi to disperse stagnation, the merit of reducing swelling and alleviating pain.
The project innovative point:
1, theory innovation thinks that primary disease belongs to the leukorrhagia category.Its pathogenesis essence is outer the invading of damp and hot evil poison, damp-heat accumulation, and the damp-stagnancy blood stasis, the retardance vessels of the uterus, blood loses due to the meat corruption.
2, method of treatment innovation: according to primary disease pathogenesis center of gravity is with damp-heat accumulation, and it is characteristic that damp-stagnancy blood stasis, retardance vessels of the uterus, blood lose meat rotten; Similar basically with the pathogenesis of boil, so adopt the Therapeutic Method of boil, clearing heat and expelling damp; Removing toxicity for eliminating carbuncles, regulating qi to disperse stagnation, reducing swelling and alleviating pain.
The specific embodiment
In conjunction with the specific embodiment, the present invention is further specified as follows:
1. listed weight proportion takes by weighing raw material required for the present invention in the according to the form below, unit: kg
<tables num="0001"> <table > <tgroup cols="5"> <colspec colname = "c001" colwidth = "17 % " /> <colspec colname="c002" colwidth="19%" /> <colspec colname="c003" colwidth="22%" /> <colspec colname =" c004 "colwidth =" 17% " /> <colspec colname="c005" colwidth="25%" /> <tbody > <row > <entry morerows="1"> </entry> <entry morerows="1"> cuneata </ entry> <entry morerows = "1 "> ground Bie insects </entry> <entry morerows="1"> Curcuma </entry> <entry morerows="1"> Siphonostegia </entry> </row> <row > <entry morerows="1"> Example 1 </entry> <entry morerows="1"> 50 </entry> <entry morerows="1"> 4 </entry> <entry morerows="1"> 5 </entry> <entry morerows = "1"> 25 </entry> </row> <row > <entry morerows="1"> Example 2 </entry> <entry morerows = "1"> 40 </entry> <entry morerows="1"> 7 </entry> <entry morerows = "1 "> 10 </entry> <entry morerows="1"> 20 </entry> </row> <row > <entry morerows = "1"> Example 3 </entry> <entry morerows="1"> 30 </entry> <entry morerows="1"> 10 </entry> <entry morerows="1"> 15 </entry> <entry morerows="1"> 15 </entry> </row> <row > <entry morerows="1"> Example 4 </entry> <entry morerows="1"> 20 </entry> <entry morerows =" 1 "> 13 </entry> <entry morerows="1"> 20 </entry> <entry morerows="1"> 10 </entry> </row> <row > <entry morerows="1"> Example 5 </entry> <entry morerows = "1" > 10 </entry> <entry morerows="1"> 18 </entry> <entry morerows="1"> 25 </entry> <entry morerows = "1"> 5 </entry> </row> </tbody> </tgroup> </table> </tables>
<tables num="0002"> <table > <tgroup cols="9"> <colspec colname = "c001" colwidth = "14 % " /> <colspec colname="c002" colwidth="10%" /> <colspec colname="c003" colwidth="11%" /> <colspec colname =" c004 "colwidth =" 8% " /> <colspec colname="c005" colwidth="9%" /> <colspec colname =" c006 "colwidth =" 11 % " /> <colspec colname="c007" colwidth="8%" /> <colspec colname="c008" colwidth="14%" /> <colspec colname =" c009 "colwidth =" 14% " /> <tbody > <row > <entry morerows="1"> </entry> <entry morerows = "1"> Patrinia </entry> <entry morerows="1"> cuneata </ entry> <entry morerows = "1"> Curcuma </entry> <entry morerows="1"> Yuan Hu </entry> <entry morerows="1"> ground Bie insects </entry> <entry morerows="1"> TPG </entry> <entry morerows="1"> moutan </entry> <entry morerows = "1"> Siphonostegia </entry> </row> <row > <entry morerows="1"> Example 6 </entry> <entry morerows="1"> 10 </entry> <entry morerows="1"> 50 </entry> <entry morerows = "1"> 5 </entry> <entry morerows="1"> 25 </entry> <entry morerows="1"> 4 </entry> <entry morerows="1"> 50 </entry> <entry morerows="1"> 5 </entry> <entry morerows = "1"> 25 </entry> </row> <row > <entry morerows="1"> Example 7 </entry> <entry morerows = "1"> 20 </entry> <entry morerows="1"> 40 </entry> <entry morerows="1"> 10 </entry> <entry morerows="1"> 20 </entry> <entry morerows="1"> 7 </entry> <entry morerows = "1"> 40 </entry> <entry morerows="1"> 10 </entry> <entry morerows="1"> 20 </entry> </row> <row > <entry morerows="1"> Example 8 </entry> <entry morerows = " 1 "> 30 </entry> <entry morerows="1"> 30 </entry> <entry morerows="1"> 15 </entry> <entry morerows="1"> 15 </entry> <entry morerows="1"> 10 </entry> <entry morerows = " 1 "> 30 </entry> <entry morerows="1"> 15 </entry> <entry morerows="1"> 15 </entry> </row> <row > <entry morerows="1"> Example 9 </entry> <entry morerows="1"> 40 </entry> <entry morerows="1"> 20 </entry> <entry morerows="1"> 20 </entry> <entry morerows =" 1 "> 10 </entry> <entry morerows="1"> 13 </entry> <entry morerows="1"> 20 </entry> <entry morerows="1"> 20 </entry> <entry morerows="1"> 10 </entry> </row> <row > <entry morerows="1"> Example 10 </entry> <entry morerows="1"> 50 </entry> <entry morerows = "1"> 10 </entry> <entry morerows="1"> 25 </entry> <entry morerows = "1" > 5 </entry> <entry morerows="1"> 18 </entry> <entry morerows="1"> 10 </entry> <entry morerows = "1"> 25 </entry> <entry morerows="1"> 5 </entry> </row> </tbody> </tgroup> </table> </tables>
Among the present invention, Herba Patriniae is all herbal medicine of Valerianaceae plant Patrinia scabiosaefolia Fisch (Patrinia scabiosaefolia Fisch.) or patrima villosa P.villosa (Thunb.) Juss; Caulis Sargentodoxae is the dry rattan of Lardizabalaceae plant Caulis Sargentodoxae Sargentodoxa cuneata (Oliv.) Rehd.et Wils..Rhizoma Curcumae is the dry rhizome of zingiberaceous plant Rhizoma Curcumae (Curcuma phaeocaulis Val.), Guangxi zedoary (Curcuma kwangsiensis S.G.Leeet C.F.Liang) or RADIX CURCUMAE (Curcuma wenyujin Y.H.Chen et C.Ling); The act like a bully tuber of papaveraceae plant corydalis Corydalis yanhusuo W.T.Wang of unit; Eupolyphaga Seu Steleophaga is the female worm dry body of Arthropoda Insecta Corydiidae (Corydi-idae) insecticide eupolyphoge sinensis (Eupolyphaga sinensis Walker) or Ji eupolyphoge sinensis (Steleophagaplancyi Boleny); Radix Paeoniae Rubra is the dry root of ranunculaceae plant Radix Paeoniae (Paeonia lactiflora Pall.) or river Radix Paeoniae Rubra (P.veitchiiLynch); Cortex Moutan is the root bark of cohosh Paeonia suffruticosa (Paeonia SuffruticosaAndrews); Herba Artemisiae Anomalae is the dry aerial parts of Compositae (Compositae) plant artemisia anomalas Artemisia anomala S.moore.
2. preparation embodiment
By proportioning raw materials among each embodiment in the last table, may further comprise the steps:
A. get the recipe quantity of Rhizoma Corydalis, put into 90%wt, each reflux, extract, 1h of 70%wt ethanol of being respectively 8～10 times of prescription weight successively, filter, merging filtrate reclaims ethanol, and medicinal residues are subsequent use;
B. get the recipe quantity of Cortex Moutan, add the water of 10～12 times of weight of prescription, soak 2～3h, adopting altogether, hydrodistillation extracts paeonol; Collect 9 times of amounts of paeonol and Cortex Moutan medical material amount saturated aqueous solution, and add the NaCl of 3% Cortex Moutan medical material amount, cold preservation 24h, sucking filtration to it; Get paeonol, the device preservation is subsequent use in addition for medicinal residues, utilizes ultrasonic method to prepare the paeonol clathrate, behind 24 times of paeonol weight dissolve with ethanol paeonol; Add 8～10 times of amount beta-schardinger dextrin-s (β-CD), supersound process 40～60min, cold preservation 24h; Sucking filtration, filtering residue are used the petroleum ether after drying, and be subsequent use;
C. get the recipe quantity of Rhizoma Curcumae, add the water of 8～10 times of prescription weight, soak 0.5～1h, the utilization steam distillation extracts volatile oil 5～6h, leaves standstill 24h, cold preservation, and it is subsequent use to keep medicinal residues;
d. taken cuneata, ground Bie insects, Patrinia, TPG, Siphonostegia prescription volume, and a, b, c Step spare dregs, add boiling water three times, respectively, followed by adding the total weight of herbs 10 times, 8 times, 8 times the amount of water, each refluxed 1h, concentrated extract to a relative density of 1.10 to 1.25, plus 60% wt ~ 70% wt of ethanol and allowed to stand refrigerated for more than 24h, filtered, the recovery of ethanol, the filtrate and the The combined solution was concentrated to continue the relative density of 1.05 to 1.20 of the extract, dried and crushed into a dry extract powder;
E. with the dry extract of d step gained and the paeonol beta-CD inclusion of above-mentioned b step gained, c step gained Rhizoma Curcumae volatile oil uniform mixing adds adjuvant again, processes required preparation.
3, preparation preferred embodiment
A: get the recipe quantity of Rhizoma Curcumae, add 8～10 times of water gagings, soak 0.5～1h, the utilization steam distillation extracts volatile oil 5～6h, leaves standstill 24h, cold preservation, and it is subsequent use to keep medicinal residues.
b: Take cuneata, ground Bie insects, Siphonostegia prescription volume, as well as a step back for the dregs, herbs were added to the amount of 10 times, 8 times and 8 times the amount of water each reflux extraction 1h, extract was concentrated to the relative density of 1.10 ~ 1.25 (60 ℃), plus 60% wt ~ 70% wt of ethanol and allowed to stand refrigerated for more than 24h, filtered, the recovery of ethanol, the filtrate was concentrated to a relative density of 1.05 to 1.20, extract, dried and crushed into a dry extract powder.
C. with the dry extract of b step gained, add adjuvant, process required preparation.
4. the preparation of various dosage forms
The granule preparation:
A. get the recipe quantity of Rhizoma Corydalis, put into 90%wt, each reflux, extract, 1h of 70%wt ethanol of being respectively 8～10 times of prescription weight successively, filter, merging filtrate reclaims ethanol, and medicinal residues are subsequent use;
B. get the recipe quantity of Cortex Moutan, add the water of 10～12 times of weight of prescription, soak 2～3h, adopting altogether, hydrodistillation extracts paeonol; Collect 9 times of amounts of paeonol and Cortex Moutan medical material amount saturated aqueous solution, and add the NaCl of 3% Cortex Moutan medical material amount, cold preservation 24h, sucking filtration to it; Get paeonol, the device preservation is subsequent use in addition for medicinal residues, utilizes ultrasonic method to prepare the paeonol clathrate, behind 24 times of paeonol weight dissolve with ethanol paeonol; Add 8～10 times of amount beta-schardinger dextrin-s (β-CD), supersound process 40～60min, cold preservation 24h; Sucking filtration, filtering residue are used the petroleum ether after drying, and be subsequent use;
C. get the recipe quantity of Rhizoma Curcumae, add the water of 8～10 times of prescription weight, soak 0.5～1h, the utilization steam distillation extracts volatile oil 5～6h, leaves standstill 24h, cold preservation, and it is subsequent use to keep medicinal residues;
d. taken cuneata, ground Bie insects, Patrinia, TPG, Siphonostegia prescription volume, and a, b, c Step spare dregs, add boiling water three times, respectively, followed by adding the total weight of herbs 10 times, 8 times, 8 times the amount of water, each refluxed 1h, concentrated extract to a relative density of 1.10 to 1.25, plus 60% wt ~ 70% wt of ethanol and allowed to stand refrigerated for more than 24h, filtered, the recovery of ethanol, the filtrate and the the combined solution was concentrated to continue the relative density of 1.05 to 1.20 of the extract, dried and crushed into a dry extract powder;
E. with the dry extract of d step gained and the paeonol beta-CD inclusion of above-mentioned b step gained; C step gained Rhizoma Curcumae volatile oil uniform mixing adds suitable adjuvant again, dextrin, soluble starch, lactose, sucrose, mannitol etc. wherein one or more; With an amount of 80% ethanol moistening; The system soft material is crossed 14 mesh sieves and is granulated 50-80.C is dry, and 60 order granulate get granule.
The preparation of tablet:
A. get the recipe quantity of Rhizoma Corydalis, put into 90%wt, each reflux, extract, 1h of 70%wt ethanol of being respectively 8～10 times of prescription weight successively, filter, merging filtrate reclaims ethanol, and medicinal residues are subsequent use;
B. get the recipe quantity of Cortex Moutan, add the water of 10～12 times of weight of prescription, soak 2～3h, adopting altogether, hydrodistillation extracts paeonol; Collect 9 times of amounts of paeonol and Cortex Moutan medical material amount saturated aqueous solution, and add the NaCl of 3% Cortex Moutan medical material amount, cold preservation 24h, sucking filtration to it; Get paeonol, the device preservation is subsequent use in addition for medicinal residues, utilizes ultrasonic method to prepare the paeonol clathrate, behind 24 times of paeonol weight dissolve with ethanol paeonol; Add 8～10 times of amount beta-schardinger dextrin-s (β-CD), supersound process 40～60min, cold preservation 24h; Sucking filtration, filtering residue are used the petroleum ether after drying, and be subsequent use;
C. get the recipe quantity of Rhizoma Curcumae, add the water of 8～10 times of prescription weight, soak 0.5～1h, the utilization steam distillation extracts volatile oil 5～6h, leaves standstill 24h, cold preservation, and it is subsequent use to keep medicinal residues;
d. taken cuneata, ground Bie insects, Patrinia, TPG, Siphonostegia prescription volume, and a, b, c Step spare dregs, add boiling water three times, respectively, followed by adding the total weight of herbs 10 times, 8 times, 8 times the amount of water, each refluxed 1h, concentrated extract to a relative density of 1.10 to 1.25, plus 60% wt ~ 70% wt of ethanol and allowed to stand refrigerated for more than 24h, filtered, the recovery of ethanol, the filtrate and the The combined solution was concentrated to continue the relative density of 1.05 to 1.20 of the extract, dried and crushed into a dry extract powder;
E. with the dry extract of d step gained and the paeonol beta-CD inclusion of above-mentioned b step gained; C step gained Rhizoma Curcumae volatile oil uniform mixing adds one or more adjuvants wherein such as starch, dextrin, Icing Sugar, lactose, microcrystalline Cellulose, mix homogeneously again; With an amount of 80% ethanol moistening; The system soft material is crossed 30 mesh sieves and is granulated, in 70～80.C is dry, with 60 mesh sieve granulate, and tabletting, sugar coating, packing, outer package, it is qualified to inspect by ready samples, gets finished product.
The preparation of oral liquid:
A. get the recipe quantity of Rhizoma Corydalis, put into 90%wt, each reflux, extract, 1h of 70%wt ethanol of being respectively 8～10 times of prescription weight successively, filter, merging filtrate reclaims ethanol, and medicinal residues are subsequent use;
B. get the recipe quantity of Cortex Moutan, add the water of 10～12 times of weight of prescription, soak 2～3h, adopting altogether, hydrodistillation extracts paeonol; Collect 9 times of amounts of paeonol and Cortex Moutan medical material amount saturated aqueous solution, and add the NaCl of 3% Cortex Moutan medical material amount, cold preservation 24h, sucking filtration to it; Get paeonol, the device preservation is subsequent use in addition for medicinal residues, utilizes ultrasonic method to prepare the paeonol clathrate, behind 24 times of paeonol weight dissolve with ethanol paeonol; Add 8～10 times of amount beta-schardinger dextrin-s (β-CD), supersound process 40～60min, cold preservation 24h; Sucking filtration, filtering residue are used the petroleum ether after drying, and be subsequent use;
C. get the recipe quantity of Rhizoma Curcumae, add the water of 8～10 times of prescription weight, soak 0.5～1h, the utilization steam distillation extracts volatile oil 5～6h, leaves standstill 24h, cold preservation, and it is subsequent use to keep medicinal residues;
d. taken cuneata, ground Bie insects, Patrinia, TPG, Siphonostegia prescription volume, and a, b, c Step spare dregs, add boiling water three times, respectively, followed by adding the total weight of herbs 10 times, 8 times, 8 times the amount of water, each refluxed 1h, concentrated extract to a relative density of 1.10 to 1.25, plus 60% wt ~ 70% wt of ethanol and allowed to stand refrigerated for more than 24h, filtered, the recovery of ethanol, the filtrate and the The combined solution was concentrated to continue the relative density of 1.18 to 1.20, with the Paeonol β-CD inclusion complex, volatile oil and mix ingredients by weight calculated: 0.15 ratio at 1:0.05 clear paste were weighed and sucrose will be made of a single sugar syrup after mixing with clear paste, adding propionic acid 0.1% or 0.1% sodium benzoate as a preservative such as 0.25%, was oral products.
The preparation of capsule:
A. get the recipe quantity of Rhizoma Corydalis, put into 90%wt, each reflux, extract, 1h of 70%wt ethanol of being respectively 8～10 times of prescription weight successively, filter, merging filtrate reclaims ethanol, and medicinal residues are subsequent use;
B. get the recipe quantity of Cortex Moutan, add the water of 10～12 times of weight of prescription, soak 2～3h, adopting altogether, hydrodistillation extracts paeonol; Collect 9 times of amounts of paeonol and Cortex Moutan medical material amount saturated aqueous solution, and add the NaCl of 3% Cortex Moutan medical material amount, cold preservation 24h, sucking filtration to it; Get paeonol, the device preservation is subsequent use in addition for medicinal residues, utilizes ultrasonic method to prepare the paeonol clathrate, behind 24 times of paeonol weight dissolve with ethanol paeonol; Add 8～10 times of amount beta-schardinger dextrin-s (β-CD), supersound process 40～60min, cold preservation 24h; Sucking filtration, filtering residue are used the petroleum ether after drying, and be subsequent use;
C. get the recipe quantity of Rhizoma Curcumae, add the water of 8～10 times of prescription weight, soak 0.5～1h, the utilization steam distillation extracts volatile oil 5～6h, leaves standstill 24h, cold preservation, and it is subsequent use to keep medicinal residues;
d. taken cuneata, ground Bie insects, Patrinia, TPG, Siphonostegia prescription volume, and a, b, c Step spare dregs, add boiling water three times, respectively, followed by adding the total weight of herbs 10 times, 8 times, 8 times the amount of water, each refluxed 1h, concentrated extract to a relative density of 1.10 to 1.25, plus 60% wt ~ 70% wt of ethanol and allowed to stand refrigerated for more than 24h, filtered, the recovery of ethanol, the filtrate and the the combined solution was concentrated to continue the relative density of 1.05 to 1.20 of the extract, dried and crushed into a dry extract powder;
E. with the dry extract of d step gained and the paeonol beta-CD inclusion of above-mentioned b step gained, c step gained Rhizoma Curcumae volatile oil uniform mixing adds moisture absorption adjuvants such as starch again; Powdered extract; Get dry extract,, cross sieve No. 6 with paeonol beta-CD inclusion mix homogeneously; Again Rhizoma Curcumae volatile oil is evenly sprayed on it, reinstall No. 1 capsule get final product finished product.
The preparation of washing liquid:
A. get the recipe quantity of Rhizoma Corydalis, put into 90%wt, each reflux, extract, 1h of 70%wt ethanol of being respectively 8～10 times of prescription weight successively, filter, merging filtrate reclaims ethanol, and medicinal residues are subsequent use;
B. get the recipe quantity of Cortex Moutan, add the water of 10～12 times of weight of prescription, soak 2～3h, adopting altogether, hydrodistillation extracts paeonol; Collect 9 times of amounts of paeonol and Cortex Moutan medical material amount saturated aqueous solution, and add the NaCl of 3% Cortex Moutan medical material amount, cold preservation 24h, sucking filtration to it; Get paeonol, the device preservation is subsequent use in addition for medicinal residues, utilizes ultrasonic method to prepare the paeonol clathrate, behind 24 times of paeonol weight dissolve with ethanol paeonol; Add 8～10 times of amount beta-schardinger dextrin-s (β-CD), supersound process 40～60min, cold preservation 24h; Sucking filtration, filtering residue are used the petroleum ether after drying, and be subsequent use;
C. get the recipe quantity of Rhizoma Curcumae, add the water of 8～10 times of prescription weight, soak 0.5～1h, the utilization steam distillation extracts volatile oil 5～6h, leaves standstill 24h, cold preservation, and it is subsequent use to keep medicinal residues;
d. taken cuneata, ground Bie insects, Patrinia, TPG, Siphonostegia prescription volume, and a, b, c Step spare dregs, add boiling water three times, respectively, followed by adding the total weight of herbs 10 times, 8 times, 8 times the amount of water, each refluxed 1h, concentrated extract to a relative density of 1.10 to 1.25, plus 60% wt ~ 70% wt of ethanol and allowed to stand refrigerated for more than 24h, filtered, the recovery of ethanol, the filtrate and the The combined solution was concentrated to continue the relative density of 1.05 to 1.20 of the extract, dried and crushed into a dry extract powder;
E. with the dry extract of d step gained and the paeonol beta-CD inclusion of above-mentioned b step gained, c step gained Rhizoma Curcumae volatile oil uniform mixing, Rhizoma Curcumae volatile oil and Tween 80 mixing; And then adding sodium benzoate; And use distilled water diluting, and regulate PH with citric acid afterwards, leave standstill; Filter, get the finished product medicinal liquid.Dilute with water is 5～10 times during use, washout, and seat is washed, and all can wash, and every day 1～2 time, a week is a course of treatment.
The preparation of suppository:
A. get the recipe quantity of Rhizoma Corydalis, put into 90%wt, each reflux, extract, 1h of 70%wt ethanol of being respectively 8～10 times of prescription weight successively, filter, merging filtrate reclaims ethanol, and medicinal residues are subsequent use;
B. get the recipe quantity of Cortex Moutan, add the water of 10～12 times of weight of prescription, soak 2～3h, adopting altogether, hydrodistillation extracts paeonol; Collect 9 times of amounts of paeonol and Cortex Moutan medical material amount saturated aqueous solution, and add the NaCl of 3% Cortex Moutan medical material amount, cold preservation 24h, sucking filtration to it; Get paeonol, the device preservation is subsequent use in addition for medicinal residues, utilizes ultrasonic method to prepare the paeonol clathrate, behind 24 times of paeonol weight dissolve with ethanol paeonol; Add 8～10 times of amount beta-schardinger dextrin-s (β-CD), supersound process 40～60min, cold preservation 24h; Sucking filtration, filtering residue are used the petroleum ether after drying, and be subsequent use;
C. get the recipe quantity of Rhizoma Curcumae, add the water of 8～10 times of prescription weight, soak 0.5～1h, the utilization steam distillation extracts volatile oil 5～6h, leaves standstill 24h, cold preservation, and it is subsequent use to keep medicinal residues;
d. taken cuneata, ground Bie insects, Patrinia, TPG, Siphonostegia prescription volume, and a, b, c Step spare dregs, add boiling water three times, respectively, followed by adding the total weight of herbs 10 times, 8 times, 8 times the amount of water, each refluxed 1h, concentrated extract to a relative density of 1.10 to 1.25, plus 60% wt ~ 70% wt of ethanol and allowed to stand refrigerated for more than 24h, filtered, the recovery of ethanol, the filtrate and the The combined solution was concentrated to continue the relative density of 1.05 to 1.20 of the extract, dried and crushed into a dry extract powder, sieve through VI, with the Paeonol β-CD inclusion complex mix will Volatile Oil and polysorbate esters -80 mix.Other gets glycerol, gelatin prepares glycerol gelatin matrix, and when molten condition, above-mentioned fine powder is joined in the extractum by the equivalent incremental method, after fully stirring, pours into suppository mould while hot, and the cooling back is taken out and got final product.
4. this preparation granules pharmacodynamic study
4.1 this preparation is to the experimentation of Mice Auricle microcirculation
4.1.2 experiment purpose: observing this preparation granules influences microcirculation of mouse auricle
4.1.2.1 experiment material
4.1.2.1.1 animal: healthy ICR mice, ♀, body weight 18-22g is provided production licence by Nanjing University of Traditional Chinese Medicine's Experimental Animal Center: SCXK (Soviet Union) 2002-0053.
4.1.2.1.2 medicine and reagent
This preparation granules (high low dose group is provided by preparation portion of Nanjing University of Traditional Chinese Medicine)
FULE KELI; The grand Chinese medicinal material industry Co., Ltd in Xiangfan specification: 18g/ bag lot number: 081202.
4.1.2.1.3 receive reagent agent amount
This preparation granules high dose is equivalent to: 0.9g/kg crude drug amount
This preparation granules low dosage is equivalent to: 0.225g/kg crude drug amount
FULE KELI is equivalent to: the former dose of 0.54g/kg
4.1.2.1.4 instrument
WT-6 type microcirculation appearance, Xuzhou optical instrument head factory is produced
4.1.2.1.5 statistical analysis
Utilize SPSS 11.0 softwares.Data are carried out one factor analysis of variance, and there is the significance meaning P＜0.05 for difference.Utilization multiple comparisons LSD method.
4.1.2.2 experimental result and method
4.1.2.2.1 method
A, be divided into four groups of this preparation granules (high low dose group), FULE KELI, blank group.One day three times for oral administration of this preparation granules, FULE KELI, a continuous week;
B, with administration one week the back by every mice 1% sodium pentobarbital 0.01mL/g intraperitoneal injection of anesthesia after,, mice ventrad is fixed on the mice observation platform a little liquid paraffin of the surperficial dropping of auricle down with medical rubber patch QUMAO on auricle.Observe administration group and the same position of blank control group auricular microcirculation blood capillary bore, blood flow speed.
4.1.2.2.2 result
This preparation granules is to microcirculation of mouse auricle influence (n=10; X ± SD, %)
Annotate: compare with blank control group: * P＜0.05, * * P＜0.01
The result shows: this preparation granules high dose group can make venule vein blood vessel caliber obviously expand (with blank control group than P＜0.05); Wherein to arteriole blood vessels caliber expansion have extremely remarkable result (with blank control group than P＜0.01), but be not clearly in the influence of arteriovenous flow velocity; This preparation granules low dose group is not obvious to the influence of Mice Auricle microcirculation; FULE KELI positive drug group Mice Auricle blood vessels caliber and flow velocity and blank control group relatively have significant difference (P＜0.05).
4.1.2.3 conclusion: this preparation granules high dose group has the effect of expanding the microcirculatory vascular caliber preferably, thereby plays the effect of microcirculation improvement.
4.2 this preparation granules is to the therapeutical effect of animal colpitis mycotica model
Colpitis mycotica is common gynecological disease, frequently-occurring disease.In colpitis mycotica, about 85-90% is caused by candida albicans infection.This preparation granules is that clinical plan is used to treat colpitis mycotica by the Liu Xuehua of the Nanjing University of Traditional Chinese Medicine professor clinical treatment vaginitis granule that is made into through proved recipe for many years.This test has been carried out pharmacodynamic study in the animal body to it, and the result shows that this preparation granules has the obvious treatment effect to the rabbit colpitis mycotica.
4.2.1 material. material and method
4.2.1.1. medicine: this preparation granules provides (water extract 2g crude drug/mL) by preparation portion of Nanjing University of Traditional Chinese Medicine; The grand Chinese medicinal material industry Co., Ltd in FULE KELI Xiangfan specification: 18g/ bag lot number: 081202; Normal saline 100mL;
4.2.1.2. white beads Seedling: provide by Microbiological Lab of Nanjing University of Traditional Chinese Medicine, be the clinical isolates strain.
4.2.1.3. animal: Japanese white big ear rabbit, female, health, body weight 2～5kg is provided by Nanjing University of Traditional Chinese Medicine's animal center.
4.2.1.4. at first set up rabbit colpitis mycotica model, the administration of dividing into groups again.Get 18 of the big ear rabbit of Japan, stretch into rabbit vagina, inoculation Candida albicans, continuous two days with scalp acupuncture leading portion flexible pipe.The 3rd heaven-made vaginal smear after inoculation for the second time, gram staining liquid dyeing, the Candida albicans microscopy is positive as a result, confirms the modeling success.Above-mentioned rabbit is divided into three groups at random.Be this preparation granules group, model group, FULE KELI group; Other gets 6 rabbit and is blank (normally) matched group.This preparation granules group and FULE KELI group are given respectively and 10mL/, and model group is refused administration.Get vaginal secretions and make plate coating checking in administration the 7th day, fortnight, 21 days inspection record vagina local patholoic change wells, the 21 day execution animal after administration.Get vagina tissue and make the pathology tissue examination.
4.2.2 result and discussion
4.2.2.1. general situation is observed: it is smooth that each organizes rabbit hair color in process of the test, feed is normal, and body weight all increases to some extent.
4.2.2.2. vagina local patholoic change perusal: rabbit vagina contrafluxion after inoculation white is read bacterium, edema, secretions increase.Pathological changes such as vagina contrafluxion, edema take a turn for the better gradually after the administration.Especially the most obvious with this preparation granules group.Obviously alleviate in treating back each administration group vagina local patholoic change on the 7th, the most recovery normally compared no significant difference with blank during to 14 days.The result sees table 1.
This preparation granules of table 1. is to the influence (n=6) of colpitis mycotica
Annotate :-: no change; +: mild hyperaemia, a small amount of secretions; ++: the moderate congestion and edema, secretions is more; +++: severe is congested, edema, and secretions is extremely many.
4.2.2.3. vaginal smear examination result sees table 2, the 7th day each the administration group smear in treatment back all looked into and seen Candida albicans.In back 14 days smears of treatment not again microscopy to Candida albicans, zero difference between group.
This preparation granules of table 2. vaginal smear result
4.3 this preparation granules extracorporeal bacteria inhibitor test
4.3.1. test material
4.3.1.1 medicine and reagent
This preparation: specification: 2g crude drug/mL, lot number:
FULE KELI; The grand Chinese medicinal material industry Co., Ltd in Xiangfan specification: 18g/ bag lot number: 081202.
The woman rather fastens: Europe, Harbin is for pharmaceutcal corporation, Ltd, specification: 1.6g/ grain lot number: 20090404
Nysfungin: Zhejiang Zhenyuan Pharmaceutical Co., Ltd, specification: 500,000 units/sheet lot number: 081005
Clotrimazole cream: Shanghai General Pharmaceutical Co., ltd., specification: 10g/ props up lot number: 090203
Penicillin sodium: Huabei Pharmaceutic Co., Ltd's specification: 800,000 units/bottle lot number: Y0805124
Norfloxacin capsule: Jiangsu Qingjiang Pharmaceutical Co., Ltd., specification: 0.1g/ grain lot number: 080802
Sodium chloride, Shanghai hundred million chemical reagent company limiteies of a specified duration, lot number: 00060120.
Nutrient broth, CAS Shanghai Insect Bio Technological Development Co., Ltd., lot number: 080223.
Nutrient agar, microorganism reagent factory of Jiangsu Prov. Disease Preventing and Controlling Center, lot number: 080026.
Calf serum, Hangzhou Sijiqing Biological Engineering Material Co., Ltd., lot number: 080508;
Take off fine sheep blood, available from sheep sheep blood supply station, Nanjing, lot number: 090802
4.3.1.2 bacterial strain
The clinical isolates strain: staphylococcus aureus, escherichia coli, B-mode micrococcus scarlatinae, Candida albicans are provided by Jiangsu Prov. People's Hospital microbial room isolation identification.
4.3.1.3 instrument and equipment
Electronic balance, Beijing Sai Duolisi balance company limited is produced;
The full-automatic high-pressure autoclave, Japanese SANYO;
Superclean bench, Suzhou Decontamination Equipment Plant;
The water isolation type constant incubator, Shanghai one permanent Science and Technology Ltd.
4.3.2. experimental technique
4.3.2.1 increasing bacterium cultivates
Get clinical isolates strain staphylococcus aureus, escherichia coli, B-mode micrococcus scarlatinae, albicans strain a little, be inoculated in respectively in the broth bouillon, cultivate 18h for 37 ℃.(adding 20% calf serum in the B-mode micrococcus scarlatinae broth bouillon) got each bacterial strain nutrient broth culture that 18h cultivates, and does the 10-3 dilution with nutrient broth and is used for experiment.
4.3.2.2 doubling dilution
[1](test tube method)
Get 11 in sterilization test tube, the 1st pipe adds nutrient broth fluid medium 1.6mL, and all the other every pipe 1mL get this preparation respectively and add in first pipe, get 1mL to the 2 pipes behind the mixing, dilution successively, and the 10th pipe sucking-off 1mL discards, and the 11st pipe does not add medicinal liquid as contrast.Every pipe adds dilution bacterium liquid 0.1mL, cultivates 24h for 37 ℃, takes out the bacterial growth situation of observing.Muddy like drug liquid tube, promptly represent bacterial growth, supply the reagent thing not have bacteriostasis; Represent then that as drug liquid tube is limpid bacterial growth is suppressed, the medicinal liquid of the greatest dilution that it can bacteria growing inhibiting is the minimal inhibitory concentration (MIC) of this medicine.It is dull and stereotyped that each pipe of limpid medicinal liquid meat soup is changeed kind of bouillon agar, cultivates 24h and observe, and the minimum drug level that does not still have bacterial growth is the bacteriocidal concentration of this medicine.
Getting FULE KELI (being made into 0.6g/mL), woman rather fastens (being made into 0.16g/mL), Nysfungin and (is made into 250,000 units/mL), penicillin sodium and (is made into 160,000 units/mL), norfloxacin capsule (being made into 0.02g/mL), as stated above; Dilution successively; Cultivate, make positive control.Clotrimazole cream is got 10mg and is made positive control.
4.3.3 result
Experimental result shows: this preparation all has stronger bacteriostasis to clinical isolates staphylococcus aureus, escherichia coli, B-mode micrococcus scarlatinae, Candida albicans; MIC shows that 5mg～100mg/mL (pressing the crude drug amount calculates) this preparation has vitro antibacterial activity.See table 1.
This preparation of table 1 antibacterial activity in vitro (n=2)
List of references
[1] Zhou Bangjing, the antibacterial action of conventional Chinese medicine and assay method thereof, scientific and technical literature publishing house Chongqing branch, 1986:298
4.4 experiment conclusion
4.4.1 this preparation granules high dose group has the effect of expanding the microcirculatory vascular caliber preferably, thereby plays the effect of microcirculation improvement
4.4.2 rabbit vagina contrafluxion after inoculation white is read bacterium, edema, secretions increase.Pathological changes such as vagina contrafluxion, edema take a turn for the better gradually after the administration.Especially the most obvious with this preparation granules group.Obviously alleviate in treating back each administration group vagina local patholoic change on the 7th, the most recovery normally compared no significant difference with blank during to 14 days.
4.4.3 this preparation all has stronger bacteriostasis to clinical isolates staphylococcus aureus, escherichia coli, B-mode micrococcus scarlatinae, Candida albicans, MIC shows that 5mg～100mg/mL (pressing the crude drug amount calculates) this preparation has vitro antibacterial activity.
Claims (1)
1. Chinese medicine that is used to treat gynecological inflammation; It is characterized in that its contained active component is prepared from the following raw materials in part by weight proportioning: Herba Patriniae 10-50 part, Caulis Sargentodoxae 10-50 part, Rhizoma Curcumae 5-25 part, Rhizoma Corydalis 5-25 part, Eupolyphaga Seu Steleophaga 4-18 part, Radix Paeoniae Rubra 10-50 part, Cortex Moutan 5-25 part, Herba Artemisiae Anomalae 5-25 part, dosage form is a granule.
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| CN102302746B (en) * | 2011-09-20 | 2014-01-29 | 浙江大学 | Traditional Chinese medicine composition for treating pelvic inflammatory disease (PID) and application thereof |
| CN103861120B (en) * | 2012-08-16 | 2015-12-09 | 湖北天圣康迪制药有限公司 | A kind of preparation method of enclose medicament |
| CN103110722B (en) * | 2013-02-25 | 2014-04-23 | 青岛大学医学院附属医院 | Traditional Chinese medicine for treating chronic pelvic inflammatory disease and preparation method thereof |
| CN103585467B (en) * | 2013-11-20 | 2015-07-08 | 艾广凤 | Medicament for treating chronic pelvic inflammation |
| CN104000954A (en) * | 2014-06-06 | 2014-08-27 | 济南科纳信息科技有限公司 | Traditional Chinese medicine (TCM) composition for treating endometritis caused by obstruction of heat toxins |
| CN104645221A (en) * | 2015-01-23 | 2015-05-27 | 王士金 | Medicine for treating obstetric infection and preparation method of medicine |
| CN105288483A (en) * | 2015-11-20 | 2016-02-03 | 吴斌 | Drug for treatment of endometritis |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1165668A (en) * | 1996-05-17 | 1997-11-26 | 贾俊英 | Medicine for treating pelvic infection |
| CN1237451A (en) * | 1999-04-01 | 1999-12-08 | 魏秀珍 | Enema liquor for woman pelvic inflammation |
| CN101152413A (en) * | 2006-09-14 | 2008-04-02 | 黄振华 | Pharmaceutical composition for gynecology inflammation and method for preparing the same |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1165668A (en) * | 1996-05-17 | 1997-11-26 | 贾俊英 | Medicine for treating pelvic infection |
| CN1237451A (en) * | 1999-04-01 | 1999-12-08 | 魏秀珍 | Enema liquor for woman pelvic inflammation |
| CN101152413A (en) * | 2006-09-14 | 2008-04-02 | 黄振华 | Pharmaceutical composition for gynecology inflammation and method for preparing the same |
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