CN101715698A

CN101715698A - Automatic production method of cordyceps mushroom capable of preventing cell carcinogenesis

Info

Publication number: CN101715698A
Application number: CN200910253716A
Authority: CN
Inventors: 曾树生
Original assignee: Individual
Current assignee: Individual
Priority date: 2009-12-09
Filing date: 2009-12-09
Publication date: 2010-06-02

Abstract

The invention belongs to the bioengineering field and has advancement and creativity embodied on the automatic production and a new product prepared by a new formulation. The automatic production mode has great yield and easily realizes production in large scale, furthermore, only a large scale production can satisfy the social requirements. The cordyceps mushroom produced by the new formulation is upgraded to an immune functional breed from an original nutrient product which is mainly based on rice, corn, starch, peptone, and the like; the culture medium of the invention comprises the following main materials: ribbonfish meal, mutton paste, Se-enriched yeast, almond powder, multi-dimensional glucose, flour weevil, fresh milk, and the like which are rich in abundant elements of selenium and germanium and anti-cancer ingredients of polysaccharides and vitamin B17 so as to greatly improve the anti-cancer effect. The invention product belongs to a new cordyceps breed with food and medical purposes, particular the food purpose, belongs to a rare product in the world, looks like a mushroom, is a new product in the field of edible mushrooms and can advance the domestic development of edible mushrooms. The invention has an important development value, thereby being an ideal project for business students and village stuff and having good development prospects.

Description

The automatic production method of cordyceps mushroom of anti-system cell carcinogenesis

Technical field

The invention belongs to bioengineering field

Technical background

The phenomenon that has occurred the counterfeit Chinese caterpillar fungus mushroom of many families in recent years in the society, these counterfeiters' prescription also rests on the basis of the living Chinese caterpillar fungus mushroom of cultivating in one's early years of original people Ceng Shu, does not have new creation, still be major ingredient preparation nutrition base with rice, corn, peptone, do not have new progress and invention.And can prevent the product of the present invention of system cell carcinogenesis having risen to truly immune class health products from nutrition type.Because the composition and the element of anti-system cancerations such as the contained polysaccharide of product of the present invention, ucleosides, selenium, element ge, all surpassed the content, particularly amygdalin composition of former Chinese caterpillar fungus mushroom.Amygdalin also claims laetrile, this is that natural cs and artificial other Chinese caterpillar funguses all do not have, the appearance of laetrile and new prescription of the present invention have direct relation, product of the present invention can be prevented the system cell carcinogenesis effectively, this with more than the existence of the active ingredient that contains and selenium, element ge relevant, it will contribute for anti-system cancer and the life that prolongs the cancer patient.

Advance of the present invention: advance is embodied in two aspects: the one, and new prescription, the 2nd, automated production.

The raw material that the novel nourishing based formulas is used, no longer based on rice, corn, but based on hairtail powder, mutton cream, fresh milk, Se-enriched yeast, almond powder, insect body fluid.This novel nourishing base is main points of the present invention, belongs to domestic initiation.

Automated production, with high content of technology, the percentage of A-class goods is improved greatly, and output is big, the mushroom sheet is abundant, and quality is good, and product look like, and help opening up markets.

Goal of the invention: be exactly to be the enhancing human immunological competence, reduce the generation and the lethality of cancer, especially lung cancer, liver cancer are prevented making with better.Simultaneously in order to fill up blank in the edible mushroom field,, and provide a short-term that DEVELOPMENT PROSPECT the arranged project of getting rich for the university students that seek the foundation project and the village officials that revitalize the agriculture-countryside-farmer for favourable impetus is played in the development of China edible mushroom.

Summary of the invention: at first test tube strains being spread cultivation is liquid spawn, change training with the 2000ml triangular flask earlier, and then spread cultivation with 100 liters of stainless steel seeding tanks, the bacterium liquid that spreads cultivation is injected large-scale nutrition base fermentation tank in proportion, advanced line space disappeared when seeding tank and nutrition base fermentation tank used, real again disappearing after the injection nutrient solution.After having cultivated nutrient solution, be transported to the auto-filling workshop again and carry out can.Produce used blake bottle with cover: high 120mm diameter 70mm volume 400ml.Before the can, all necessary strict sterilization of automation racking room and equipment and blake bottle, racking room must be a sterilized space, computer is handled.The loading amount control of nutrient solution is at 150ml in the blake bottle, and the blake bottle that can is good is checked whether good seal of bottle cap, is transported to and cultivates the workshop, is emitted on the culturing rack, cultivates.Temperature in the culturing room is controlled between 18-22 ℃, and 50 flying worm straw mushrooms just can generate.

Concrete implementing process content: at first by the test tube strains liquid spawn that spreads cultivation, use the triangular flask of 2000ml volume earlier, pour into the 1000ml fresh milk after cleaning, add 30 gram dusty yeasts again, 50 gram peptones, glucose 20 grams, seal with the high-temperature resistance plastice film, sterilize, sterilising temp 120-121 ℃, 40 minutes effective sterilizing time, afterwards, when treating that temperature is reduced to 15-18 ℃, under aseptic condition with invisible spectro mycelia picking 2-3 fritter, be placed on gently on the liquid nutritional base in the triangular flask, quiet training three days when treating that the bacterial classification edge bears new mycelia, just is placed on triangular flask on the bottle swingging machine, carry out shaken cultivation, frequency of oscillation per minute 50-60 time is after 7 days, after the bacterium ball in seeing triangular flask is full of nutrient solution, the failure of oscillations, and pour into immediately in the seeding tank of 100 liter capacities, before pouring into, seeding tank will carry out the high temperature sky earlier and disappear, sky disappears temperature 130-135 ℃, 60 minutes.After 60 minutes, when treating jar temperature drop to 60-70 ℃, pour into the nutrient solution for preparing, and carry out reality and disappear, the effective temperature that disappears in fact is controlled at 120-121 ℃, disinfecting time 40 minutes.Afterwards, when treating that a jar interior temperature is reduced to 20-25 ℃, again the bacterium liquid in the seeding tank is injected fermentation tank, the injection ratio is that 10%, 100 kilogram of nutrient solution injects 10 kilograms of bacterium liquid.Inject the nutrient solution prefermentor and also will carry out the real work that disappears of sky slake, sky disappears, the real temperature and time that disappears is identical with seeding tank.Select for use fermentation tank with 5000 liter capacities for well, the nutrient solution injection rate is 4000 liters, accounts for 80% of volume.Ratio in 5 ‰ in the nutrient solution adds defoamer, and the pH value nature is pressed 10% of nutrient solution then and added liquid spawn, afterwards, just starts agitator and air compressor machine, and agitator Revolution Per Minute 150-160 changes, and the air inflow of air compressor machine is 1: 0.5 cube.Check the bacterium pearl in the nutrient solution after 7 days, as can wash out 80-100 bacterium ball among every ml the time, just stop stirring, and can immediately nutrient input be delivered to the auto-filling workshop, carry out can.The amount of pouring into control is checked after the can whether the sealing situation of cultivating bottle cap is good, and then is transported to the cultivation workshop at 150ml in each blake bottle, is emitted on the culturing rack, cultivates.Culturing room's temperature remains between 18-22 ℃, humidity 60-65%.Incubation time 50 days just can be gathered to after date, refrigerates then and dries, and packing is sold again.

Chinese caterpillar fungus Pseudomonas ascus class subphylum used in the present invention, spherical shell ridge, meat seat order, Clavicipitaceae, Cordyceps, middle name Cordyceps militaris Verticillium dahliae, latin name: verticillium stat cordyceps militaris (L.) link.

Be used to produce in batches the nutrient solution compound method of Chinese caterpillar fungus mushroom: with 100 kilograms of fresh milks is the preparation standard, wherein add 10 kilograms of mutton cream, 10 kilograms of almond powder, 5 kilograms of Se-enriched yeast powder, 20 gram VB1 powder, 10 kilograms of fresh and alive yellow mealworm body fluid, 50 gram potassium dihydrogen phosphates, 500 gram multivita-glucoses, 5 kilograms of hairtail powder (hairtail decaptitate tail, intestines tripe, thin squama, clean and be dried into powder).Be miscible in together with fresh milk then, pour into fermentation tank.The consumption size of Chinese caterpillar fungus mushroom nutrient solution, proportional quantity according to 100 kilograms of fresh milks increases and reduces, the amount of pouring into accounts for 80% of fermentation tank volume, the addition of bacterium liquid accounts for 10% of nutrient solution, as to adopt 5000 liters of fermentation tanks to pour into nutrient solution be 4000 liters, add 400 liters of liquid spawns in 4000 liters of nutrient solutions, ratio is 10%.Ratio in 5 ‰ in the nutrient solution adds defoamer, the pH value nature, and the adding prefermentor will carry out sky and disappear, sky disappears temperature 130-135 ℃, and the perfusion nutrient solution carried out reality and disappears when jar temperature drop was to 60-70 ℃ after 60 minutes after pouring into, disappear temperature 120-121 ℃ in fact, 40 minutes, afterwards, when treating jar temperature drop to 20-25 ℃, add bacterium liquid in proportion, afterwards, just start agitator and air compressor machine, mixing speed per minute 150-160 changes, 1: 0.5 cube of air compressor air intake amount, after 7 days, when every ml can wash out 80-100 Cordyceps Militaris ball in the culture fluid, stop to cultivate, again nutrient input is delivered to the auto-filling workshop, start transfer matic, carry out can, each blake bottle can amount 150ml in aseptic condition down, whether the close inspection bottle cap seals after the can, and then deliver between cultivation and cultivate, the temperature control is at 18-22 ℃ between cultivation, and 50 flying worm straw mushrooms just can grow up to.

More than be the whole technology contents of the present invention.

Claims

1. characteristics of the present invention are following whole technology content

A, the making of liquid spawn: at first by the test tube strains liquid spawn that spreads cultivation, use the triangular flask of 2000ml volume earlier, pour into the 1000ml fresh milk after cleaning, add 30 gram dusty yeasts again, 50 gram peptones, glucose 20 grams seal with the high-temperature resistance plastice film, sterilize, sterilising temp 120-121 ℃, 40 minutes effective sterilizing time, afterwards, when treating that temperature is reduced to 15-18 ℃, under aseptic condition with invisible spectro mycelia picking 2-3 fritter, be placed on gently on the liquid nutritional base in the triangular flask, quiet training three days is when treating that the bacterial classification edge bears new mycelia, just triangular flask is placed on the bottle swingging machine, carry out shaken cultivation, frequency of oscillation per minute 50-60 time is after 7 days, after bacterium ball in seeing triangular flask is full of nutrient solution, the failure of oscillations, and pour into immediately in the seeding tank of 100 liter capacities, before pouring into, seeding tank will carry out the high temperature sky earlier and disappear, sky disappears temperature 130-135 ℃, and 60 minutes, after 60 minutes, when treating jar temperature drop to 60-70 ℃, pour into the nutrient solution for preparing, and carry out reality and disappear, the effective temperature that disappears in fact is controlled at 120-121 ℃, disinfecting time 40 minutes, afterwards, when treating that a jar interior temperature is reduced to 20-25 ℃, again the bacterium liquid in the seeding tank is injected fermentation tank;

B, produce the making of Chinese caterpillar fungus mushroom nutrient solution in batches: with 100 kilograms of fresh milks is the preparation standard, wherein add 10 kilograms of mutton cream, 10 kilograms of almond powders, 5 kilograms of Se-enriched yeast powder, 20 gram VB1 powder, 10 kilograms of fresh and alive yellow mealworm body fluid, 50 gram potassium dihydrogen phosphates, 500 gram multivita-glucoses, 5 kilograms of hairtail powder (hairtail tail of decaptitating, the intestines tripe, thin squama, clean and be dried into powder), be miscible in together with fresh milk then, pour into fermentation tank, the consumption size of Chinese caterpillar fungus mushroom nutrient solution, proportional quantity according to 100 kilograms of fresh milks increases and reduces, the amount of pouring into accounts for 80% of fermentation tank volume, the addition of bacterium liquid accounts for 10% of nutrient solution, as to adopt 5000 liters of fermentation tanks to pour into nutrient solution be 4000 liters, add 400 liters of liquid spawns in 4000 liters of nutrient solutions, ratio is 10%, ratio in 5 ‰ in the nutrient solution adds defoamer, the pH value nature, the adding prefermentor will carry out sky and disappear, sky disappears temperature 130-135 ℃, perfusion nutrient solution when jar temperature drop is to 60-70 ℃ after 60 minutes, carrying out reality after pouring into disappears, disappear temperature 120-121 ℃ in fact, 40 minutes, afterwards, when treating jar temperature drop to 20-25 ℃, add bacterium liquid in proportion, just start agitator and air compressor machine afterwards, mixing speed per minute 150-160 changes, 1: 0.5 cube of air compressor air intake amount, after 7 days, when every ml can wash out 80-100 Cordyceps Militaris ball in the culture fluid, nutrient solution culture was made merit, just can pour into blake bottle and produce the Chinese caterpillar fungus mushroom;

C, this sends out Chinese caterpillar fungus Pseudomonas ascus class subphylum used in the present invention, spherical shell ridge, meat seat order, Clavicipitaceae, Cordyceps, middle name Cordyceps militaris Verticillium dahliae, latin name: verticillium stat cordyceps militaris (L.) link, the Chinese caterpillar fungus mushroom meat of producing with this kind bacterium is thick tender, and product look like.