CN108998399A - Wastewater treatment microbial microbial inoculum and preparation method thereof - Google Patents
Wastewater treatment microbial microbial inoculum and preparation method thereof Download PDFInfo
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C02F3/00—Biological treatment of water, waste water, or sewage
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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- C12N1/18—Baker's yeast; Brewer's yeast
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Abstract
The present invention relates to the technical field of wastewater treatment, especially a kind of wastewater treatment microbial microbial inoculum and preparation method thereof, microbial bacterial agent is made of the component of following parts by weight: 20~50 parts of microbial inoculum；18~30 parts of talcum powder；18~30 parts of yeast powder；25~55 parts of earthworm powder；15~28 parts of leech powder；16~27 parts of hydrolyzed pearl powder；26~34 parts of charcoal powder；Strain being combined by one or more of Lactococcus lactis, Eubacterium dolichum, lactobacillus plantarum, bacillus coagulans, brewer's yeast, saccharomyces cerevisiae, streptococcus faecalis, mucor, unconcerned extra large prestige bacillus, Cellvibrio and denitrifying bacterium in the microbial inoculum.The wastewater treatment microbial microbial inoculum and preparation method thereof can completely remove the organic pollutant in water.
Description
Technical field
The present invention relates to the technical field of wastewater treatment, especially a kind of wastewater treatment microbial microbial inoculum and its preparation side
Method.
Background technique
Wastewater treatment is handled waste water using the method for physics, chemistry and biology, makes purification of waste water, reduces pollution,
So that reaching Sewage treatment, multiplexing, water resource is made full use of.Waste water includes sanitary wastewater and industrial wastewater, at present the place of waste water
Reason method has: physical treatment process, method of chemical treatment and biological treatment.Biological treatment therein using more and more extensive,
But the effect of most of biological treatment is less desirable, water quality that treated remain unchanged can exist it is many it is toxic at
Part, seriously affect the material life of people.
Summary of the invention
The technical problem to be solved by the present invention is being provided a kind of useless to solve the problems, such as above-mentioned background technique
Water treatment micro-organism microbial inoculum and preparation method thereof can completely remove the organic pollutant in water.
The technical solution adopted by the present invention to solve the technical problems is: a kind of wastewater treatment microbial microbial inoculum, described
Microbial bacterial agent is made of the component of following parts by weight:
20~50 parts of microbial inoculum；18~30 parts of talcum powder；18~30 parts of yeast powder；25~55 parts of earthworm powder；15~28 parts of leech powder；
16~27 parts of hydrolyzed pearl powder；26~34 parts of charcoal powder；Strain is by Lactococcus lactis, Eubacterium dolichum, plant in the microbial inoculum
Object lactobacillus, bacillus coagulans, brewer's yeast, saccharomyces cerevisiae, streptococcus faecalis, mucor, unconcerned extra large prestige bacillus, fiber arc
One or more of bacterium and denitrifying bacterium are combined into.
A kind of preparation method for preparing wastewater treatment microbial microbial inoculum, comprising the following steps:
Step 1: by Lactococcus lactis, Eubacterium dolichum, lactobacillus plantarum, bacillus coagulans, brewer's yeast, saccharomyces cerevisiae,
One or more of streptococcus faecalis, mucor, unconcerned extra large prestige bacillus, Cellvibrio and denitrifying bacterium are inoculated into solid
It is cultivated on culture medium, obtains activated spawn；
Step 2: activated spawn is inoculated in liquid medium and is cultivated, draws suitable fluid nutrient medium using aseptic straw,
It is added dropwise in container, gently vibrates, so that suspension is presented in fluid nutrient medium, take suspension；
Step 3: talcum powder, yeast powder, earthworm powder, leech powder, hydrolyzed pearl powder, charcoal powder are uniformly mixed with suspension, and
Sterile water is added, shaken cultivation obtains mixed liquor；
Step 4: mixed liquor being at the uniform velocity added to crosslinking curing in barium hydroxide and hydrochloric acid mixed solution and obtains gel ball, use is sterile
It is added in solution of chlorogenic acid after water washing, dry after mixing evenly, freezing obtains use in waste water treatment microbial bacterial agent.
Further specifically, the preparation process of the solid medium is in above-mentioned technical proposal: by mutton cream,
Peptone and sodium chloride are all dissolved into distilled water, using sodium hydroxide solution by pH value adjustment to 7~7.6, in the liquid
It is middle clastic agar is added and seals sterilized in merging disinfection equipment, obtain solid medium after its cooling.
Further specifically, the preparation process of the liquid medium is in above-mentioned technical proposal: high-temperature digestion fine jade
The hydraulic seal is placed in disinfection equipment and sterilizes, obtain using sodium hydroxide solution by pH value adjustment to 7.2~7.5 by rouge
To liquid medium.
Further specifically, in above-mentioned technical proposal, the weight ratio of the activated spawn and liquid medium
For 1.2~1.5:1.
Further specifically, in above-mentioned technical proposal, the weight ratio of the suspension and mixed liquor is 1.5~
2:1。
Further specifically, in above-mentioned technical proposal, the mixed liquor and barium hydroxide and hydrochloric acid mixed solution
Weight ratio is 2~3:1.
The beneficial effects of the present invention are: a kind of wastewater treatment microbial microbial inoculum provided by the invention and preparation method thereof, gives up
Water treatment efficiency significantly improves, and accelerates water body decomposition velocity of organic substances, can improve water quality.
Specific embodiment
In order to which the technical problems, technical solutions and beneficial effects solved by the present invention is more clearly understood, below in conjunction with
Embodiment, the present invention will be described in further detail.It should be appreciated that specific embodiment described herein is only used to explain
The present invention is not intended to limit the present invention.
Embodiment 1
A kind of wastewater treatment microbial microbial inoculum, microbial bacterial agent are made of the component of following parts by weight:
20 parts of microbial inoculum；18 parts of talcum powder；18 parts of yeast powder；25 parts of earthworm powder；15 parts of leech powder；16 parts of hydrolyzed pearl powder；Charcoal
26 parts of powder；8 parts of strain is combined by Lactococcus lactis, 8 parts of Eubacterium dolichum, 4 parts of lactobacillus plantarum in microbial inoculum.
A kind of preparation method for preparing wastewater treatment microbial microbial inoculum, comprising the following steps:
Step 1: 8 parts of Lactococcus lactis, 8 parts of Eubacterium dolichum, 4 parts of lactobacillus plantarum are inoculated into solid culture respectively
It is cultivated on base, obtains activated spawn；
Step 2: activated spawn is inoculated in liquid medium and is cultivated, draws suitable fluid nutrient medium using aseptic straw,
It is added dropwise in container, gently vibrates, so that suspension is presented in fluid nutrient medium, take suspension；Activated spawn and liquid medium
Weight ratio be 1.2:1.
Step 3: talcum powder, yeast powder, earthworm powder, leech powder, hydrolyzed pearl powder, charcoal powder are mixed with suspension
It is even, and sterile water is added, shaken cultivation obtains mixed liquor；The weight ratio of suspension and mixed liquor is 1.5:1；
Step 4: mixed liquor being at the uniform velocity added to crosslinking curing in barium hydroxide and hydrochloric acid mixed solution and obtains gel ball, use is sterile
It is added in solution of chlorogenic acid after water washing, dry after mixing evenly, freezing obtains use in waste water treatment microbial bacterial agent；Mixed liquor
It is 2:1 with the weight ratio of barium hydroxide and hydrochloric acid mixed solution.
Wherein, the preparation process of solid medium is: mutton cream, peptone and sodium chloride are all dissolved into distilled water
In, using sodium hydroxide solution by pH value adjustment to 7, clastic agar is added in the liquid and seals merging disinfection equipment
In sterilized, after its cooling after obtain solid medium.
The preparation process of liquid medium is: high-temperature digestion agar, using sodium hydroxide solution by pH value adjustment to 7.2,
The hydraulic seal is placed in disinfection equipment and is sterilized, liquid medium is obtained.
A kind of wastewater treatment microbial microbial inoculum provided by the invention and preparation method thereof, water treatment effect significantly improves,
Accelerate water body decomposition velocity of organic substances, water quality can be improved.
Embodiment 2
A kind of wastewater treatment microbial microbial inoculum, microbial bacterial agent are made of the component of following parts by weight:
30 parts of microbial inoculum；20 parts of talcum powder；20 parts of yeast powder；30 parts of earthworm powder；20 parts of leech powder；20 parts of hydrolyzed pearl powder；Charcoal
30 parts of powder；Strain is combined by 10 parts of bacillus coagulans, 10 parts of brewer's yeast, 10 parts of saccharomyces cerevisiae in microbial inoculum.
A kind of preparation method for preparing wastewater treatment microbial microbial inoculum, comprising the following steps:
Step 1: 10 parts of bacillus coagulans, 10 parts of brewer's yeast, 10 parts of saccharomyces cerevisiae are inoculated into solid training respectively
It supports and is cultivated on base, obtain activated spawn；
Step 2: activated spawn is inoculated in liquid medium and is cultivated, draws suitable fluid nutrient medium using aseptic straw,
It is added dropwise in container, gently vibrates, so that suspension is presented in fluid nutrient medium, take suspension；Activated spawn and liquid medium
Weight ratio be 1.3:1.
Step 3: talcum powder, yeast powder, earthworm powder, leech powder, hydrolyzed pearl powder, charcoal powder are mixed with suspension
It is even, and sterile water is added, shaken cultivation obtains mixed liquor；The weight ratio of suspension and mixed liquor is 1.6:1；
Step 4: mixed liquor being at the uniform velocity added to crosslinking curing in barium hydroxide and hydrochloric acid mixed solution and obtains gel ball, use is sterile
It is added in solution of chlorogenic acid after water washing, dry after mixing evenly, freezing obtains use in waste water treatment microbial bacterial agent；Mixed liquor
It is 2.2:1 with the weight ratio of barium hydroxide and hydrochloric acid mixed solution.
Wherein, the preparation process of solid medium is: mutton cream, peptone and sodium chloride are all dissolved into distilled water
In, using sodium hydroxide solution by pH value adjustment to 7.2, clastic agar is added in the liquid and seals merging sterilization sets
It is sterilized in standby, obtains solid medium after its cooling.
The preparation process of liquid medium is: high-temperature digestion agar, using sodium hydroxide solution by pH value adjustment to 7.3,
The hydraulic seal is placed in disinfection equipment and is sterilized, liquid medium is obtained.
A kind of wastewater treatment microbial microbial inoculum provided by the invention and preparation method thereof, water treatment effect significantly improves,
Accelerate water body decomposition velocity of organic substances, water quality can be improved.
Embodiment 3
A kind of wastewater treatment microbial microbial inoculum, microbial bacterial agent are made of the component of following parts by weight:
40 parts of microbial inoculum；25 parts of talcum powder；25 parts of yeast powder；40 parts of earthworm powder；25 parts of leech powder；25 parts of hydrolyzed pearl powder；Charcoal
32 parts of powder；Strain is combined by 20 parts of streptococcus faecalis, 10 parts of mucor, the extra large prestige bacillus of 10 parts of desert in microbial inoculum.
A kind of preparation method for preparing wastewater treatment microbial microbial inoculum, comprising the following steps:
Step 1: 20 parts of streptococcus faecalis, 10 parts of mucor, the extra large prestige bacillus of 10 parts of desert are inoculated into solid training respectively
It supports and is cultivated on base, obtain activated spawn；
Step 2: activated spawn is inoculated in liquid medium and is cultivated, draws suitable fluid nutrient medium using aseptic straw,
It is added dropwise in container, gently vibrates, so that suspension is presented in fluid nutrient medium, take suspension；Activated spawn and liquid medium
Weight ratio be 1.4:1.
Step 3: talcum powder, yeast powder, earthworm powder, leech powder, hydrolyzed pearl powder, charcoal powder are mixed with suspension
It is even, and sterile water is added, shaken cultivation obtains mixed liquor；The weight ratio of suspension and mixed liquor is 1.8:1.
Step 4: mixed liquor being at the uniform velocity added to crosslinking curing in barium hydroxide and hydrochloric acid mixed solution and obtains gel ball, is used
It is added in solution of chlorogenic acid after sterile water washing, dry after mixing evenly, freezing obtains use in waste water treatment microbial bacterial agent.It is mixed
The weight ratio for closing liquid and barium hydroxide and hydrochloric acid mixed solution is 2.5:1.
Wherein, the preparation process of solid medium is: mutton cream, peptone and sodium chloride are all dissolved into distilled water
In, using sodium hydroxide solution by pH value adjustment to 7.3, clastic agar is added in the liquid and seals merging sterilization sets
It is sterilized in standby, obtains solid medium after its cooling.
The preparation process of liquid medium is: high-temperature digestion agar, using sodium hydroxide solution by pH value adjustment to 7.4,
The hydraulic seal is placed in disinfection equipment and is sterilized, liquid medium is obtained.
A kind of wastewater treatment microbial microbial inoculum provided by the invention and preparation method thereof, water treatment effect significantly improves,
Accelerate water body decomposition velocity of organic substances, water quality can be improved.
Embodiment 4
A kind of wastewater treatment microbial microbial inoculum, microbial bacterial agent are made of the component of following parts by weight:
50 parts of microbial inoculum；30 parts of talcum powder；30 parts of yeast powder；55 parts of earthworm powder；28 parts of leech powder；27 parts of hydrolyzed pearl powder；Charcoal
34 parts of powder；In microbial inoculum strain by 25 parts the extra large prestige bacillus of desert, 10 parts of Cellvibrio and 15 parts of denitrifying bacterium in conjunction with and
At.
A kind of preparation method for preparing wastewater treatment microbial microbial inoculum, comprising the following steps:
Step 1: the extra large prestige bacillus of 25 parts of desert, 10 parts of Cellvibrio and 15 parts of denitrifying bacterium are inoculated into solid respectively
It is cultivated on culture medium, obtains activated spawn；
Step 2: activated spawn is inoculated in liquid medium and is cultivated, draws suitable fluid nutrient medium using aseptic straw,
It is added dropwise in container, gently vibrates, so that suspension is presented in fluid nutrient medium, take suspension；Activated spawn and liquid medium
Weight ratio be 1.5:1.
Step 3: talcum powder, yeast powder, earthworm powder, leech powder, hydrolyzed pearl powder, charcoal powder are mixed with suspension
It is even, and sterile water is added, shaken cultivation obtains mixed liquor；The weight ratio of suspension and mixed liquor is 2:1.
Step 4: mixed liquor being at the uniform velocity added to crosslinking curing in barium hydroxide and hydrochloric acid mixed solution and obtains gel ball, is used
It is added in solution of chlorogenic acid after sterile water washing, dry after mixing evenly, freezing obtains use in waste water treatment microbial bacterial agent.It is mixed
The weight ratio for closing liquid and barium hydroxide and hydrochloric acid mixed solution is 3:1.
Wherein, the preparation process of solid medium is: mutton cream, peptone and sodium chloride are all dissolved into distilled water
In, using sodium hydroxide solution by pH value adjustment to 7.6, clastic agar is added in the liquid and seals merging sterilization sets
It is sterilized in standby, obtains solid medium after its cooling.
The preparation process of liquid medium is: high-temperature digestion agar, using sodium hydroxide solution by pH value adjustment to 7.5,
The hydraulic seal is placed in disinfection equipment and is sterilized, liquid medium is obtained.
A kind of wastewater treatment microbial microbial inoculum provided by the invention and preparation method thereof, water treatment effect significantly improves,
Accelerate water body decomposition velocity of organic substances, water quality can be improved.
More than, it is merely preferred embodiments of the present invention, but scope of protection of the present invention is not limited thereto, it is any
Those familiar with the art in the technical scope disclosed by the present invention, according to the technique and scheme of the present invention and its invents
Design is subject to equivalent substitution or change, should be covered by the protection scope of the present invention.
Claims (7)
1. a kind of wastewater treatment microbial microbial inoculum, it is characterised in that: the microbial bacterial agent by following parts by weight component group
At:
20~50 parts of microbial inoculum；18~30 parts of talcum powder；18~30 parts of yeast powder；25~55 parts of earthworm powder；15~28 parts of leech powder；
16~27 parts of hydrolyzed pearl powder；26~34 parts of charcoal powder；Strain is by Lactococcus lactis, Eubacterium dolichum, plant in the microbial inoculum
Object lactobacillus, bacillus coagulans, brewer's yeast, saccharomyces cerevisiae, streptococcus faecalis, mucor, unconcerned extra large prestige bacillus, fiber arc
One or more of bacterium and denitrifying bacterium are combined into.
2. a kind of preparation method for preparing wastewater treatment microbial microbial inoculum as described in claim 1, it is characterised in that: packet
Include following steps:
Step 1: by Lactococcus lactis, Eubacterium dolichum, lactobacillus plantarum, bacillus coagulans, brewer's yeast, saccharomyces cerevisiae,
One or more of streptococcus faecalis, mucor, unconcerned extra large prestige bacillus, Cellvibrio and denitrifying bacterium are inoculated into solid
It is cultivated on culture medium, obtains activated spawn；
Step 2: activated spawn is inoculated in liquid medium and is cultivated, draws suitable fluid nutrient medium using aseptic straw,
It is added dropwise in container, gently vibrates, so that suspension is presented in fluid nutrient medium, take suspension；
Step 3: talcum powder, yeast powder, earthworm powder, leech powder, hydrolyzed pearl powder, charcoal powder are uniformly mixed with suspension, and
Sterile water is added, shaken cultivation obtains mixed liquor；
Step 4: mixed liquor being at the uniform velocity added to crosslinking curing in barium hydroxide and hydrochloric acid mixed solution and obtains gel ball, use is sterile
It is added in solution of chlorogenic acid after water washing, dry after mixing evenly, freezing obtains use in waste water treatment microbial bacterial agent.
3. preparation method according to claim 2, it is characterised in that: the preparation process of the solid medium is: will
Mutton cream, peptone and sodium chloride are all dissolved into distilled water, using sodium hydroxide solution by pH value adjustment to 7~7.6,
Clastic agar is added in the liquid and seals and is sterilized in merging disinfection equipment, obtains solid culture after its cooling
Base.
4. preparation method according to claim 2, it is characterised in that: the preparation process of the liquid medium is: high
Temperature dissolution agar, using sodium hydroxide solution by pH value adjustment to 7.2~7.5, by the hydraulic seal be placed in disinfection equipment into
Row sterilization, obtains liquid medium.
5. preparation method according to claim 2, it is characterised in that: the weight of the activated spawn and liquid medium
Part ratio is 1.2~1.5:1.
6. preparation method according to claim 2, it is characterised in that: the weight ratio of the suspension and mixed liquor
For 1.5~2:1.
7. preparation method according to claim 2, it is characterised in that: the mixed liquor and barium hydroxide and mixed in hydrochloric acid
The weight ratio of solution is 2~3:1.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101715698A (en) * | 2009-12-09 | 2010-06-02 | 曾树生 | Automatic production method of cordyceps mushroom capable of preventing cell carcinogenesis |
| CN106947722A (en) * | 2017-04-28 | 2017-07-14 | 连云港中新污水处理有限公司 | Microbial bacterial agent and preparation method thereof and the application in sewage disposal |
| CN108101228A (en) * | 2017-12-13 | 2018-06-01 | 浙江海洋大学 | A kind of wastewater treatment microbial microbial inoculum |
-
2018
- 2018-09-07 CN CN201811042373.XA patent/CN108998399A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101715698A (en) * | 2009-12-09 | 2010-06-02 | 曾树生 | Automatic production method of cordyceps mushroom capable of preventing cell carcinogenesis |
| CN106947722A (en) * | 2017-04-28 | 2017-07-14 | 连云港中新污水处理有限公司 | Microbial bacterial agent and preparation method thereof and the application in sewage disposal |
| CN108101228A (en) * | 2017-12-13 | 2018-06-01 | 浙江海洋大学 | A kind of wastewater treatment microbial microbial inoculum |
Non-Patent Citations (2)
| Title |
|---|
| SIVA SABARATNAM ET AL.: "Formulation of a Streptomyces Biocontrol Agent for the Suppression of Rhizoctonia Damping-off in Tomato Transplants", 《BIOLOGICAL CONTROL》 * |
| 阳桂菊: "固定化复合芽孢杆菌微生态制剂产品研制", 《中国优秀硕士学位论文全文数据库 工程科技Ⅰ辑》 * |
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