JP5445822B2 - Microorganisms exhibiting organic waste decomposing action, microbial composition, organic waste decomposing method, and compost producing method - Google Patents

Description

  The present invention relates to a microorganism exhibiting an organic waste decomposing action, a microbial composition, a method for decomposing organic waste, and a method for producing compost.

  Conventional microbial compositions exhibiting organic waste decomposition and organic waste decomposition methods include Bacillus subtilis, Bacillus sp.KB-02, Bacillus barismortis, Bacillus licheniformis, Bacillus pumilus There is a technique for decomposing organic waste at about 40 ° C. to about 65 ° C. using a microbial composition containing The purpose of this technology is to decompose organic waste in an extremely short time with little generation of malodor during and after decomposition.

Japanese Patent Laid-Open No. 2003-9848

  The present invention uses microorganisms different from the prior art, generates less malodor, can decompose organic waste in a very short time, and exhibits microorganisms, microorganism compositions, and organic wastes that exhibit organic waste decomposition action. It aims at providing the decomposition method and the manufacturing method of compost.

In order to achieve the above object, a microorganism exhibiting an organic waste decomposing action according to the present invention is characterized by comprising a Bacillus sp. THU3710 strain (FERM P-21616).
Another microorganism having an organic waste decomposing action according to the present invention is characterized by comprising a Bacillus ginsengihumi strain THU4501 (FERM P-21617).
Furthermore, another microorganism according to the present invention exhibiting an organic waste decomposing action is characterized by comprising a Bacillus sp. THU0021 strain (FERM P-21615).
The microorganism composition exhibiting the organic waste decomposing action according to the present invention includes Paenibacillus sp. THU0001 strain (FERM P-21614), Bacillus sp. THU3710 strain (FERM P-21616), Including Bacillus ginsengihumi strain THU4501 (FERM P-21617), Bacillus sp. THU0021 strain (FERM P-21615) and Brevibacterium sp. And
Each microorganism was entrusted to the Patent Organism Depositary, National Institute of Advanced Industrial Science and Technology, on July 23, 2008, and each deposit number was Paenibacillus sp. THU0001 strain FERM P-21614, Bacillus sp. (Bacillus sp.) THU3710 strain is FERM P-21616, Bacillus ginsengihumi THU4501 strain is FERM P-21617, and Bacillus sp. THU0021 strain is FERM P-21615.

In the method for decomposing organic waste according to the present invention, the organic waste is mixed with Bacillus sp. THU3710 strain (FERM P-21616) or the microbial composition, and kept at a temperature of 50 ° C to 75 ° C. It is characterized by doing.
In the method for producing compost according to the present invention, organic waste is mixed with Bacillus sp. THU3710 strain (FERM P-21616) or the microbial composition and kept at a temperature of 50 ° C to 75 ° C. It is characterized by.

  According to the present invention, by using a microbial composition different from the prior art, the generation of malodors and the ability to decompose organic waste in a very short time, microorganisms having an organic waste decomposition action, microbial compositions, A method for decomposing organic waste and a method for producing compost can be provided.

The microorganism exhibiting the organic waste decomposing action of the embodiment of the present invention is characterized by comprising a Bacillus sp. THU3710 strain (FERM P-21616). Another microorganism exhibiting the organic waste decomposing action of the embodiment of the present invention is characterized by comprising a Bacillus ginsengihumi strain THU4501 (FERM P-21617). Yet another microorganism exhibiting the organic waste decomposing action of the embodiment of the present invention is characterized by comprising a Bacillus sp. THU0021 strain (FERM P-21615).
The microorganism composition exhibiting the organic waste decomposing action of the embodiment of the present invention includes Paenibacillus sp. THU0001 strain (FERM P-21614) and Bacillus sp. THU3710 strain (FERM P- 21616), Bacillus ginsengihumi strain THU4501 (FERM P-21617), Bacillus sp. THU0021 strain (FERM P-21615) and Brevibacterium sp. It is characterized by that.

In the method for decomposing organic waste according to the embodiment of the present invention, the organic waste is mixed with Bacillus sp. THU3710 strain (FERM P-21616) or the microbial composition, and the mixture is mixed at 50 ° C to 75 ° C. It is characterized by being kept warm at temperature.
In the method for producing compost according to the embodiment of the present invention, an organic waste is mixed with a Bacillus sp. THU3710 strain (FERM P-21616) or the microbial composition, and a temperature of 50 ° C. to 75 ° C. It is characterized by keeping warm.

  In the method for decomposing organic waste and the method for producing compost according to the embodiment of the present invention, it is preferable to place the organic waste under acidic conditions of pH 4 or less. The heat retention temperature is preferably 50 ° C. to 75 ° C., particularly 55 ° C. to 65 ° C. The water content at the start of the treatment of the organic waste is preferably in the range of about 10% by weight to about 80% by weight, particularly preferably about 20% to about 70%. In the method for decomposing organic waste and the method for producing compost of the embodiment of the present invention, when the microorganism or the microorganism composition is mixed with the organic waste, the mixture is stirred and the microorganism and the organic waste are mixed well. It is preferable to supply oxygen.

(A) Mycological properties of each microorganism The mycological properties of each microorganism are as follows.
Based on the following mycological properties, each strain was searched by the 9th edition of the “Bergery's Mannual of Determinative Bacteriology” and identified as a new type of microorganism.

1) Paenibacillus sp. THU0001 (FERM P-21614)
Gram-positive bacilli and aerobic colony morphology (use medium: normal agar medium, culture temperature: 37 ° C., culture days: 2 days), diameter: 3 mm, color: cream, shape: round, raised state: half-lens, peripheral : Full edge, surface shape, etc .: Smooth, Transparency: Translucent, Viscosity: Butter-like Spore-forming ability.
There is no report on the utilization of carbohydrates.
CECAPO6 is 28 ° C, whereas 37 ° C-45 ° C is the optimal growth temperature.
CECAPO6 is positive, whereas the oxidase test is negative
16S rDNA sequence comparison is not identical to Paenibacillus rhizosphaerae CECAP06, which has the highest identity (99.9% homology: 1460/1462)
The homology with the 16S rDNA sequence of other strains is as follows.
Identity of Paenibacillus cineris strain LMG18439 with 16S rRNA 99.7%
Identity of Paenibacillus favisporus GMP03 strain with 16S rRNA 99.7%
Identity of Paenibacillus larvae subsp. Larvae DSM7030 strain with 16S rRNA 93.8%

The partial sequence (1462 bp) of the DNA encoding 16S ribosomal RNA of this strain is as follows (see Sequence Listing).

2) Bacillus sp. THU3710 (FERM P-21616)
Gram-positive bacilli / aerobic colony morphology (use medium: normal agar medium, culture temperature: 37 ° C., culture days: 2 days), diameter: 1 mm, color: cream, shape: punctate, raised state: flat, peripheral : Full edge, surface shape, etc .: rough, transparency: opaque, consistency: butter-like Spore-forming ability.
16S rDNA sequence comparison is not identical to Bacillus sp. B8W22, which has the highest identity (99.9% homology: 1473/1474)
The homology with the 16S rDNA sequence of other strains is as follows.
Identity of Bacillus megaterium MPF-906 strain with DNA encoding 16S ribosomal RNA 99.9%
Identity of Bacillaceae bacterium KVD-unk-56 strain with DNA encoding 16S ribosomal RNA 99.9%
Identity of Bacillus cereus ATCC14579 strain with DNA encoding 16S ribosomal RNA 94.3%
Identity of Bacillus anthracis ATCC 4229 strain with 16S rRNA 94.4%

The partial sequence (1473 bp) of the DNA encoding 16S ribosomal RNA of this strain is as follows (see Sequence Listing).

3) Bacillus ginsengihumi THU4501 (FERM P-21617)
Gram-positive bacilli and aerobic colony morphology (used medium: MRS, culture temperature: 37 ° C., culture days: 2 days), diameter: 3 mm, color: cream, shape: round, raised state: flat, peripheral edge: whole edge Surface shape, etc .: rough, transparency: opaque, consistency: butter-like Spore-forming ability.
This bacteria Reference bacteria Glucose assimilation ＋ −
Rhamnose + −
Mannitol + −
Sorbitol + −
Oxidase − +
Catalase + −
Same as Bacillus ginsengihumi Gsoil 114 (100% homology: 1464/1464) with the highest identity in 16S rDNA sequence comparison
The homology with the 16S rDNA sequence of other strains is as follows.
Identity of Bacillus sp. YX strain with DNA encoding 16S ribosomal RNA 99.9%
Identity of Bacillus sp. PA23 strain with DNA encoding 16S ribosomal RNA 99.9%
Identity of Bacillus cereus ATCC14579 strain with DNA encoding 16S ribosomal RNA 94.5%
Identity of Bacillus anthracis ATCC 4229 strain with 16S rRNA 94.6%

The partial sequence (1464 bp) of the DNA encoding 16S ribosomal RNA of this strain is as follows (see Sequence Listing).

4) Brevibacterium sp.
Gram-positive bacilli and aerobic spore-forming ability.

5) Bacillus sp. THU0021 (FERM P-21615)
Gram-positive bacilli / aerobic colony morphology (use medium: normal agar medium, culture temperature: 37 ° C., culture days: 2 days), diameter: 1 mm, color: cream, shape: punctate, raised state: flat, peripheral : Full edge, surface shape, etc .: rough, transparency: opaque, consistency: butter-like Spore-forming ability.
Same as Bacillaceae bacterium KVD-1982-07 (100% homology: 1480/1480) with the highest identity in comparison of 16S rDNA sequences
The homology with the 16S rDNA sequence of other strains is as follows.
Identity of Bacillus circulans N3 strain with 16S rRNA 99.9%
Identity of Bacillus circulans strain ATCC 4513 strain with 16S rRNA 99.5%
Identity of Bacillus cereus ATCC 14579 with 16S rRNA 94.5%
Identity of Bacillus anthracis ATCC 4229 strain with 16S rRNA 94.2%

The partial sequence (1480 bp) of the DNA encoding the 16S ribosomal RNA of this strain is as follows (see Sequence Listing).

(B) Isolation of each microorganism Each microorganism was isolated on June 22, 2007 from a composting apparatus in Aizawa Manufacturing Co., Ltd. 6-53 Sakae 2-chome, Tagajo City, Miyagi Prefecture. First, compost during fermentation was collected from an operating composting apparatus, suspended in sterilized water, applied to a medium as shown below, cultured at 37-50 ° C, and each was isolated.
1) Paenibacillus sp. THU0001
2) Bacillus sp. THU3710
5) Bacillus sp. THU0021
These three strains were isolated on Difco's Lactobacilli MRS medium with agar or solid medium with gellan gum.
The composition of the medium, the culture temperature, and the culture time are as follows.
Medium DIFCO Lactobacilli MRS medium (containing 1% agar or kelan gum)
Incubation temperature 37-50 ℃
Incubation time 1-2 days 3) Bacillus ginsengihumi THU4501
4) Brevibacterium sp. THU0002
These two strains were finally isolated on a normal agar medium or a normal medium (Nutrient broth) using gellan gum.
The composition of the medium, the culture temperature, and the culture time are as follows.
Medium Normal medium (contains 1% meat extract, 1% peptone, 0.3% NaCl, and 1.5% agar or kelan gum)
Incubation temperature 37-50 ℃
Incubation time 1-2 days

(C) Acid production of each microorganism 1) Paenibacillus sp. THU0001, 2) Bacillus sp. THU3710, 3) Bacillus ginsengihumi THU4501, 5) Bacillus sp. THU0021 was confirmed to produce acid.
In order to confirm the production of acid, 0.3% by weight of calcium carbonate was mixed in the agar medium shown in FIG. Each microorganism was inoculated on each agar medium and cultured. When the bacteria released acid, the calcium carbonate dissolved and the surroundings of the bacteria became transparent, confirming the production of acid.
As a result, the production of acid was confirmed in the above four strains.
In the decomposition of organic waste, the generation of acid is important, and it has the effect of lowering the pH of the organic waste and suppressing the rot odor. In the case where the microbial composition showing the organic waste decomposition action consists of 5 strains, 1) Paenibacillus sp. THU0001 occupies about 90% of the total number of bacteria in an example in which the rot odor is effectively suppressed and decomposed. The other 4 strains account for about 5-10% of the total number of cells. However, the occupation ratio of each bacterium is not limited to the range.

(D) Usefulness of microorganisms
A microbial composition having an organic waste decomposition action containing Paenibacillus sp. THU0001 (FERM P-21614) and Bacillus sp. THU3710 (FERM P-21616) is mixed with the organic waste, and the mixture is heated at a temperature of 50 ° C to 75 ° C. By keeping the temperature, it is possible to decompose organic waste while suppressing the rot odor such as ammonia. When Bacillus ginsengihumi THU4501 (FERM P-21617) is further included in the microbial composition, it can be decomposed more effectively while suppressing the rot odor. In the case where Brevibacterium sp. THU0002 and Bacillus sp. THU0021 (FERM P-21615) are further included in the microbial composition, it is possible to more effectively suppress the rot odor and stably decompose.

  In the microbial composition showing the organic waste decomposing action of the embodiment of the present invention, the organic waste decomposing method and the compost producing method, as organic waste suitable for decomposing, vegetable waste, fish waste, etc. Examples include, but are not limited to, food residues, fallen leaves, wood chips, and manure.

According to the embodiment of the present invention, the following actions and effects can be obtained.
1) Organic waste can be decomposed in a very short time. In addition, stable and continuous treatment is possible by supplying organic waste every predetermined time, for example, every 24 hours.
2) Since other microorganisms (so-called miscellaneous bacteria) do not grow because the pH is low, it does not easily rot and hardly causes malodor such as ammonia during processing. Even if the manufactured compost is left as it is, no odor such as ammonia is generated.

[Organic waste decomposition experiment]
(A) Organic waste decomposition experiment by each microorganism In order to investigate what kind of enzymes each of the five types of bacteria secrete and decompose organic waste, shake the filter medium or vegetables in the bacterial culture solution. Cultures were examined to see if degradation occurred.
[experimental method]
Paenibacillus sp. THU0001 (normal medium) (meat extract 1%, peptone 1%, NaCl 0.3%), Brevibacterium sp. THU0002 (MRS medium) (trade name Lactobacillus MRS broth, manufacturer DIFCO), Bacillus ginsengihumi THU4501 ( MRS medium) (trade name Lactobasiri MRS broth, manufacturer DIFCO), Bacillus sp. THU0021 (V-YEM medium) (vegetable juice 8%, K ₂ HPO ₄ 0.5%, MgSO ₄ 7H ₂ O 0.2%, NaCl 0.1% , Mannitol 5.0%, Na-gluconate 5.0%, yeast extract 0.5%), Bacillus sp. THU 3710 (ordinary medium) (meat extract 1%, peptone 1%, NaCl 0.3%) After culturing for 24 hours, each non-sterilized raw vegetable (daikon radish and carrot chopped one by one) was placed in 5 ml of each of the obtained culture solutions, and further cultured at 60 ° C with shaking. In addition, in order to see the synergistic effect of bacteria, 1 ml of the culture solution of each fungus was added to a total of 5 ml of liquid, and the same unsterilized vegetables were also cultured with shaking at 60 ° C and 45 ° C. . As a control for comparison, the same experiment as described above was performed using 5 ml of a total of 5 ml of a normal medium, 2 ml of MRS medium, and 1 ml of V-YEM medium.

[Experimental result]
Degradation of vegetables was clearly observed in Paenibacillus sp. THU0001 and Bacillus sp. THU 3710, which were added with 5 types of bacteria shaken at 60 ° C. In particular, the radish in Paenibacillus sp. Almost disappeared. From the addition of 5 types of bacteria shaken at 60 ° C, there was no ammonia odor or other bad odor. In addition, a spoiled odor was produced from the addition of 5 types of bacteria shaken at 45 ° C.
We confirmed that vegetables were decomposed in liquid cultures, and it was proved at the test tube level that the decomposition of vegetables occurred at 60 ° C when 5 types of cultures were mixed. At 60 ° C, there was no ammonia odor or other bad odor. At 45 ° C., a decaying odor occurred without decomposition. Probably due to contamination by bacteria.
In particular, Paenibacillus sp. THU0001 can decompose a vegetable to a liquid state alone at 60 ° C. even in an open system. That is, it can be decomposed without the influence of various bacteria. Bacillus sp. THU3710 is weak but has the same degrading power.

(B) Organic waste decomposition experiment with microbial composition using two types of microorganisms [Experimental method]
(1) Place 200 ml of compost powder (two times steam sterilized) into a beaker.
(2) Two sets of 35 g were prepared including carrot (with skin) 15 g, radish (with skin) 10 g, burdock (with skin) 10 g. About 30% of the root vegetable burdock was used because of its low moisture content and difficulty in decomposition.
(3) Bacillus sp. THU3710
Paenibacillus sp.THU0001
20 ml of each of the two types of cultures separately cultured in total (40 ml) and 10 ml of water were added to the specimen, and 40 ml of medium alone and 10 ml of water were added to the control.
(4) The sample was transferred to a stainless steel box (5 cm × 15 cm × 8 cm), and 10 ml of beaker washing water was added and stirred.
(5) It put into 60 degreeC thermostat simultaneously. In addition, for comparison purposes, only vegetables in a petri dish without using compost powder were placed in a 60 ° C. incubator at the same time, and the states were compared.
(6) The mixture was stirred once or twice per hour until the third hour.
Thereafter, the mixture was stirred in a timely manner at 60 ° C. until the second day while adjusting the water content (range of 20 to 40% by mass).
From the 3rd day to the 5th day, it was set as the stationary state.

[Experimental result]
Three days later, the remaining vegetables were taken out, washed with water, and then wiped with a paper. Table 1 shows the weights of the remaining vegetables measured and compared.

During the experiment, almost no odor was generated from the control. In the case of adding two kinds of bacteria, no ammonia odor or other bad odor was generated.
From this result, it can be seen that organic waste can be efficiently decomposed in a short time by a combination of Bacillus sp. THU3710 and Paenibacillus sp. THU0001.

(C) Organic waste decomposition experiment with microbial composition using three types of microorganisms [Experimental method]
(1) Place 200 ml of compost powder (two times steam sterilized) into a beaker.
(2) Two sets of 35 g were prepared including carrot (with skin) 15 g, radish (with skin) 10 g, burdock (with skin) 10 g. About 30% of the root vegetable burdock was used because of its low moisture content and difficulty in decomposition.
(3) Bacillus ginsengihumi THU4501
Bacillus sp.THU3710
Paenibacillus sp.THU0001
10 ml of each of the three types of culture media separately cultured in total (30 ml) and 30 ml of water were added to the specimen, and 30 ml of medium alone and 30 ml of water were added to the control.
(4) The sample was transferred to a stainless steel box (5 cm × 15 cm × 8 cm), and 10 ml of beaker washing water was added and stirred.
(5) It put into 60 degreeC thermostat simultaneously. In addition, for comparison purposes, only vegetables in a petri dish without using compost powder were placed in a 60 ° C. incubator at the same time, and the states were compared.
(6) The mixture was stirred once or twice per hour until the third hour.
Thereafter, the mixture was stirred in a timely manner at 60 ° C. until the second day while adjusting the water content (range of 20 to 40% by mass).
From the 3rd day to the 5th day, it was set as the stationary state.

[Experimental result]
Four days later, the remaining vegetables were taken out, washed with water, and then wiped with a paper.
Compared to the case where only vegetables were allowed to stand at 60 ° C for 4 days and the control mixed with only compost powder without the addition of bacteria, the sample containing 3 types of bacterial cultures clearly reduced the amount of vegetables. It was clear that composting was promoted.
Next, the weight of the extracted vegetables was measured and compared with each other.

During the experiment, almost no odor was generated from the control. In the case of adding three kinds of bacteria, no ammonia odor or other bad odor was generated.
This result shows that organic waste can be efficiently decomposed in a short time by a combination of Bacillus ginsengihumi THU4501, Bacillus sp. THU3710, and Paenibacillus sp. THU0001.

(D) Organic waste decomposition experiment with microbial composition using five types of microorganisms [Experimental method]
(1) Place 200 ml of compost powder (two times steam sterilized) into a beaker.
(2) Two sets of a total of 35 g of vegetables, 5 g of radish, 10 g of radish (edible part), 5 g of carrot skin, 10 g of carrot (edible part) and 5 g of mizuna were prepared.
(3) Paenibacillus sp. THU0001 (FERM P-21614), Bacillus sp. THU3710 (FERM P-21616), Bacillus ginsengihumi THU4501 (FERM P-21617), Brevibacterium sp. THU0002, Bacillus sp. THU0021 (FERM P -21615) were separately cultured, and 10 ml of each of the five types of culture solutions (50 ml in total) was added to the specimen. (In this case, Brevibacterium sp. THU0002 and Bacillus ginsengihumi THU 4501 were MRS medium, Bacillus sp. THU3710 and Paenibacillus sp. THU0001 were Neutrient broth, Bacillus sp. THU0021 was 1% mannitol, 8% V8, Each was cultured in 0.3% calcium carbonate medium.)
(4) The sample was transferred to a stainless steel box (5 cm × 15 cm × 8 cm), and 10 ml of beaker washing water was added and stirred.
(5) It put into 60 degreeC thermostat simultaneously.
(6) The mixture was stirred once or twice per hour until the third hour.
Thereafter, the mixture was stirred in a timely manner at 60 ° C. until the second day while adjusting the water content (range of 20 to 40% by mass).
From the 3rd day to the 5th day, it was set as the stationary state.

[Experimental result]
The remaining vegetables after 50 hours and 5 days were removed with tweezers and placed in a petri dish to compare the progress of composting.
The vegetables taken out after 50 hours and 5 days were washed with water, wiped with a paper, and then weighed. In addition, the vegetables after 5 days were obtained by returning the vegetables after 50 hours to the stainless steel box again and subsequently composting. The results are shown in Table 3.

  From the above results, it was confirmed that the addition of the cultures of the five types of strains, as compared with the control, clearly showed faster degradation and disappearance from both weight and appearance. In addition, when the change in weight is observed, the control also shows a considerable change. This is because the moisture content was reduced by drying as a result of continuing the treatment at 60 ° C., and the weight was reduced.

During the experiment, almost no odor was generated from the control. In the case of adding 5 kinds of bacteria, no ammonia odor or other bad odor was generated.
From this result, Paenibacillus sp. THU0001 (FERM P-21614), Bacillus sp. THU3710 (FERM P-21616), Bacillus ginsengihumi THU4501 (FERM P-21617), Brevibacterium sp. THU0002, Bacillus sp. THU0021 (FERM It can be seen that organic waste can be efficiently decomposed in a short time by combining with P-21615).

For 1) Paenibacillus sp. THU0001, 2) Bacillus sp. THU3710, 3) Bacillus ginsengihumi THU4501, 5) Bacillus sp. THU0021, the relationship between the degree of bacterial growth (OD660) and pH is shown in the graphs of FIGS. 3) As for Bacillus ginsengihumi THU4501, the relationship between the degree of growth (OD660) and pH when buffering capacity is given is also shown in the graph of FIG.
As shown in FIGS. 1 to 4, when any strain enters the logarithmic growth phase, it produces acid and lowers the pH. Since two strains, 1) Paenibacillus sp. THU0001 and 3) Bacillus ginsengihumi THU4501, can grow even when the pH drops, they can start to produce acid in the logarithmic growth phase and continue to grow. On the other hand, 5) Bacillus sp. THU0021 strain is considered to stop growing when it starts to produce acid by itself, starts to grow when pH exceeds 5, and resistant bacteria continue to grow. 2) If Bacillus sp. THU3710 is not buffered, its growth is inhibited by its own acid and cannot grow at all. In a medium having a buffering capacity, a decrease in pH is suppressed, so that growth is possible. However, growth is not completely restored by acid generation. Therefore, 2) Bacillus sp. THU3710 strain is considered to be more sensitive to acid than 5) Bacillus sp. THU0021 strain.
From the above, 1) Paenibacillus sp. THU0001, 2) Bacillus sp. THU3710, 3) Bacillus ginsengihumi THU4501, 5) Bacillus sp. THU0021, respectively, reduces the pH to about 4.5-5 in logarithmic growth. I understand.

It is a graph which shows the growth degree (OD660) and pH of Paenibacillus sp. THU0001 used in the Example of this invention. It is a graph which shows the growth degree (OD660) and pH of Bacillus sp. THU3710 used in the Example of this invention. It is a graph which shows the growth degree (OD660) and pH of Bacillus ginsengihumi THU4501 used in the Example of this invention. It is a graph which shows the growth degree (OD660) of Bacillus sp. THU0021 used in the Example of this invention, and pH. It is a graph which shows the growth degree (OD660) at the time of giving the buffer capacity of Bacillus sp. THU3710 used in the Example of this invention, and pH.

Claims (7)

Bacillus sp. THU3710 strain (FERM P-21616), which is a microorganism exhibiting an organic waste decomposing action. Paenibacillus sp. THU0001 strain (FERM P-21614), Bacillus sp. THU3710 strain (FERM P-21616) and Bacillus ginsengihumi THU4501 strain (FERM P-21617) And a Bacillus sp. THU0021 strain (FERM P-21615) and Brevibacterium sp. A method for decomposing organic waste, wherein the microorganism according to claim 1 is mixed with organic waste and kept at a temperature of 50 to 75 ° C. A method for decomposing organic waste, wherein the microorganism according to claim 1 or the microorganism composition according to claim 2 is mixed with the organic waste, and the mixture is kept at a temperature of 50 ° C to 75 ° C. A method for producing compost, wherein the microorganism according to claim 1 or the microorganism composition according to claim 2 is mixed with organic waste, and the mixture is kept at a temperature of 50 ° C to 75 ° C. Bacillus ginsengihumi THU4501 strain (FERM P-21617), which is an organic waste-decomposing microorganism. Bacillus sp. THU0021 strain (FERM P-21615), which is a microorganism exhibiting an organic waste decomposing action.

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