CN101691555B - 游离细胞或固定化细胞微生物转化生产l-鸟氨酸的方法 - Google Patents
游离细胞或固定化细胞微生物转化生产l-鸟氨酸的方法 Download PDFInfo
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- CN101691555B CN101691555B CN2009101764929A CN200910176492A CN101691555B CN 101691555 B CN101691555 B CN 101691555B CN 2009101764929 A CN2009101764929 A CN 2009101764929A CN 200910176492 A CN200910176492 A CN 200910176492A CN 101691555 B CN101691555 B CN 101691555B
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- ornithine
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- bacillus thuringiensis
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- AHLPHDHHMVZTML-BYPYZUCNSA-N L-Ornithine Chemical compound NCCC[C@H](N)C(O)=O AHLPHDHHMVZTML-BYPYZUCNSA-N 0.000 title claims abstract description 190
- AHLPHDHHMVZTML-UHFFFAOYSA-N Orn-delta-NH2 Natural products NCCCC(N)C(O)=O AHLPHDHHMVZTML-UHFFFAOYSA-N 0.000 title claims abstract description 95
- 229960003104 ornithine Drugs 0.000 title claims abstract description 95
- 210000001822 immobilized cell Anatomy 0.000 title claims abstract description 46
- 210000004027 cell Anatomy 0.000 title claims abstract description 35
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 25
- 230000001131 transforming effect Effects 0.000 title claims abstract description 7
- 244000005700 microbiome Species 0.000 title abstract description 6
- 241000193388 Bacillus thuringiensis Species 0.000 claims abstract description 74
- 229940097012 bacillus thuringiensis Drugs 0.000 claims abstract description 74
- 239000007788 liquid Substances 0.000 claims abstract description 30
- 238000000034 method Methods 0.000 claims abstract description 26
- 230000009466 transformation Effects 0.000 claims abstract description 26
- 238000006243 chemical reaction Methods 0.000 claims description 53
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 30
- 238000000855 fermentation Methods 0.000 claims description 28
- 230000004151 fermentation Effects 0.000 claims description 28
- 239000012530 fluid Substances 0.000 claims description 27
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims description 25
- 230000001580 bacterial effect Effects 0.000 claims description 24
- 238000000926 separation method Methods 0.000 claims description 24
- 238000002156 mixing Methods 0.000 claims description 21
- 238000012545 processing Methods 0.000 claims description 20
- 238000000746 purification Methods 0.000 claims description 20
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 19
- 229910019142 PO4 Inorganic materials 0.000 claims description 19
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 19
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 19
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 19
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 19
- 239000004202 carbamide Substances 0.000 claims description 19
- 235000005822 corn Nutrition 0.000 claims description 19
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical class [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 19
- 239000008103 glucose Substances 0.000 claims description 19
- 235000012204 lemonade/lime carbonate Nutrition 0.000 claims description 19
- 235000021317 phosphate Nutrition 0.000 claims description 19
- 150000003013 phosphoric acid derivatives Chemical class 0.000 claims description 19
- 229910052700 potassium Inorganic materials 0.000 claims description 19
- 239000011591 potassium Substances 0.000 claims description 19
- 238000002360 preparation method Methods 0.000 claims description 18
- 239000002054 inoculum Substances 0.000 claims description 16
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 claims description 15
- 239000000499 gel Substances 0.000 claims description 14
- 238000006555 catalytic reaction Methods 0.000 claims description 13
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- 229920001817 Agar Polymers 0.000 claims description 12
- 239000008272 agar Substances 0.000 claims description 12
- NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 claims description 11
- 238000011534 incubation Methods 0.000 claims description 11
- 238000011081 inoculation Methods 0.000 claims description 11
- 239000003456 ion exchange resin Substances 0.000 claims description 11
- 229920003303 ion-exchange polymer Polymers 0.000 claims description 11
- LSNNMFCWUKXFEE-UHFFFAOYSA-M Bisulfite Chemical compound OS([O-])=O LSNNMFCWUKXFEE-UHFFFAOYSA-M 0.000 claims description 10
- ODKSFYDXXFIFQN-BYPYZUCNSA-N L-arginine Chemical compound OC(=O)[C@@H](N)CCCN=C(N)N ODKSFYDXXFIFQN-BYPYZUCNSA-N 0.000 claims description 10
- 229930064664 L-arginine Natural products 0.000 claims description 10
- 235000014852 L-arginine Nutrition 0.000 claims description 10
- 239000011324 bead Substances 0.000 claims description 10
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- 238000002425 crystallisation Methods 0.000 claims description 10
- 230000008025 crystallization Effects 0.000 claims description 10
- 238000012258 culturing Methods 0.000 claims description 10
- 230000006837 decompression Effects 0.000 claims description 10
- 239000000839 emulsion Substances 0.000 claims description 10
- 239000011259 mixed solution Substances 0.000 claims description 10
- 230000002572 peristaltic effect Effects 0.000 claims description 10
- 239000000843 powder Substances 0.000 claims description 10
- 238000001291 vacuum drying Methods 0.000 claims description 10
- 238000009423 ventilation Methods 0.000 claims description 10
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 claims description 9
- 235000010413 sodium alginate Nutrition 0.000 claims description 9
- 239000000661 sodium alginate Substances 0.000 claims description 9
- 229940005550 sodium alginate Drugs 0.000 claims description 9
- 241000894006 Bacteria Species 0.000 claims description 8
- 239000011521 glass Substances 0.000 claims description 8
- 239000000758 substrate Substances 0.000 claims description 8
- 239000000725 suspension Substances 0.000 claims description 8
- BVKZGUZCCUSVTD-UHFFFAOYSA-N carbonic acid Chemical compound OC(O)=O BVKZGUZCCUSVTD-UHFFFAOYSA-N 0.000 claims description 7
- 239000012141 concentrate Substances 0.000 claims description 7
- 230000003245 working effect Effects 0.000 claims description 6
- 102000004190 Enzymes Human genes 0.000 claims description 5
- 108090000790 Enzymes Proteins 0.000 claims description 5
- 241000238631 Hexapoda Species 0.000 claims description 5
- 239000002253 acid Substances 0.000 claims description 5
- 238000013019 agitation Methods 0.000 claims description 5
- 238000004061 bleaching Methods 0.000 claims description 5
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 claims description 5
- 239000004327 boric acid Substances 0.000 claims description 5
- 239000003729 cation exchange resin Substances 0.000 claims description 5
- 229940023913 cation exchange resins Drugs 0.000 claims description 5
- 230000001413 cellular effect Effects 0.000 claims description 5
- 238000005119 centrifugation Methods 0.000 claims description 5
- 238000001816 cooling Methods 0.000 claims description 5
- 238000012856 packing Methods 0.000 claims description 5
- 239000000575 pesticide Substances 0.000 claims description 5
- 210000001082 somatic cell Anatomy 0.000 claims description 5
- 241000170793 Phalaris canariensis Species 0.000 claims description 4
- 230000000813 microbial effect Effects 0.000 claims description 4
- 239000004372 Polyvinyl alcohol Substances 0.000 claims description 3
- 231100000572 poisoning Toxicity 0.000 claims description 3
- 230000000607 poisoning effect Effects 0.000 claims description 3
- 229920002451 polyvinyl alcohol Polymers 0.000 claims description 3
- JOYRKODLDBILNP-UHFFFAOYSA-N Ethyl urethane Chemical compound CCOC(N)=O JOYRKODLDBILNP-UHFFFAOYSA-N 0.000 claims description 2
- 108010010803 Gelatin Proteins 0.000 claims description 2
- 229920002125 Sokalan® Polymers 0.000 claims description 2
- 229920000159 gelatin Polymers 0.000 claims description 2
- 239000008273 gelatin Substances 0.000 claims description 2
- 235000019322 gelatine Nutrition 0.000 claims description 2
- 235000011852 gelatine desserts Nutrition 0.000 claims description 2
- 229920003023 plastic Polymers 0.000 claims description 2
- 235000019422 polyvinyl alcohol Nutrition 0.000 claims description 2
- 239000011347 resin Substances 0.000 claims description 2
- 229920005989 resin Polymers 0.000 claims description 2
- 229910001220 stainless steel Inorganic materials 0.000 claims description 2
- 239000010935 stainless steel Substances 0.000 claims description 2
- 241000209149 Zea Species 0.000 claims 2
- 125000000635 L-ornithyl group Chemical class [H]N([H])[C@]([H])(C(=O)[*])C([H])([H])C([H])([H])C(N([H])[H])([H])[H] 0.000 claims 1
- 239000007864 aqueous solution Substances 0.000 claims 1
- 230000001954 sterilising effect Effects 0.000 claims 1
- 238000004659 sterilization and disinfection Methods 0.000 claims 1
- 239000012535 impurity Substances 0.000 abstract description 4
- 238000010924 continuous production Methods 0.000 abstract description 3
- 239000001963 growth medium Substances 0.000 abstract 1
- 240000008042 Zea mays Species 0.000 description 17
- 239000002609 medium Substances 0.000 description 13
- 239000004475 Arginine Substances 0.000 description 6
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 6
- 235000009697 arginine Nutrition 0.000 description 6
- 238000006460 hydrolysis reaction Methods 0.000 description 5
- 230000007062 hydrolysis Effects 0.000 description 4
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 description 3
- 241000500437 Plutella xylostella Species 0.000 description 3
- 230000003833 cell viability Effects 0.000 description 3
- 238000012216 screening Methods 0.000 description 3
- 239000006152 selective media Substances 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 2
- 230000002950 deficient Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000012262 fermentative production Methods 0.000 description 2
- 231100000219 mutagenic Toxicity 0.000 description 2
- 230000003505 mutagenic effect Effects 0.000 description 2
- 238000004321 preservation Methods 0.000 description 2
- 230000001018 virulence Effects 0.000 description 2
- 102000004452 Arginase Human genes 0.000 description 1
- 108700024123 Arginases Proteins 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 241000186146 Brevibacterium Species 0.000 description 1
- 239000004215 Carbon black (E152) Substances 0.000 description 1
- 241000186226 Corynebacterium glutamicum Species 0.000 description 1
- 241000218691 Cupressaceae Species 0.000 description 1
- 241000012249 Dendrolimus spectabilis Species 0.000 description 1
- KIWBPDUYBMNFTB-UHFFFAOYSA-N Ethyl hydrogen sulfate Chemical compound CCOS(O)(=O)=O KIWBPDUYBMNFTB-UHFFFAOYSA-N 0.000 description 1
- ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 description 1
- RHGKLRLOHDJJDR-BYPYZUCNSA-N L-citrulline Chemical compound NC(=O)NCCC[C@H]([NH3+])C([O-])=O RHGKLRLOHDJJDR-BYPYZUCNSA-N 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 240000001307 Myosotis scorpioides Species 0.000 description 1
- RHGKLRLOHDJJDR-UHFFFAOYSA-N Ndelta-carbamoyl-DL-ornithine Natural products OC(=O)C(N)CCCNC(N)=O RHGKLRLOHDJJDR-UHFFFAOYSA-N 0.000 description 1
- 241001147398 Ostrinia nubilalis Species 0.000 description 1
- VQXSOUPNOZTNAI-UHFFFAOYSA-N Pyrethrin I Natural products CC(=CC1CC1C(=O)OC2CC(=O)C(=C2C)CC=C/C=C)C VQXSOUPNOZTNAI-UHFFFAOYSA-N 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 230000007073 chemical hydrolysis Effects 0.000 description 1
- 235000013477 citrulline Nutrition 0.000 description 1
- 229960002173 citrulline Drugs 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- QPJDMGCKMHUXFD-UHFFFAOYSA-N cyanogen chloride Chemical compound ClC#N QPJDMGCKMHUXFD-UHFFFAOYSA-N 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 229930195733 hydrocarbon Natural products 0.000 description 1
- 150000002430 hydrocarbons Chemical class 0.000 description 1
- 239000002917 insecticide Substances 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
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- 231100000350 mutagenesis Toxicity 0.000 description 1
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- 239000003960 organic solvent Substances 0.000 description 1
- 230000010355 oscillation Effects 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- HYJYGLGUBUDSLJ-UHFFFAOYSA-N pyrethrin Natural products CCC(=O)OC1CC(=C)C2CC3OC3(C)C2C2OC(=O)C(=C)C12 HYJYGLGUBUDSLJ-UHFFFAOYSA-N 0.000 description 1
- VJFUPGQZSXIULQ-XIGJTORUSA-N pyrethrin II Chemical compound CC1(C)[C@H](/C=C(\C)C(=O)OC)[C@H]1C(=O)O[C@@H]1C(C)=C(C\C=C/C=C)C(=O)C1 VJFUPGQZSXIULQ-XIGJTORUSA-N 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
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Abstract
Description
Claims (10)
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CN2009101764929A CN101691555B (zh) | 2009-09-17 | 2009-09-17 | 游离细胞或固定化细胞微生物转化生产l-鸟氨酸的方法 |
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CN2009101764929A CN101691555B (zh) | 2009-09-17 | 2009-09-17 | 游离细胞或固定化细胞微生物转化生产l-鸟氨酸的方法 |
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CN101691555A CN101691555A (zh) | 2010-04-07 |
CN101691555B true CN101691555B (zh) | 2011-11-09 |
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CN105175275B (zh) * | 2015-07-01 | 2017-03-01 | 山东民强生物科技股份有限公司 | 一种l‑鸟氨酸的分离纯化方法 |
CN105368913B (zh) * | 2015-12-22 | 2019-11-08 | 滨海瀚鸿生化有限公司 | 用于工业化生产手性非天然氨基酸的双酶制备法 |
CN105886566A (zh) * | 2016-05-10 | 2016-08-24 | 宁波市镇海海德生化科技有限责任公司 | 一种微生物酶法转化生产l-鸟氨酸的方法 |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0393708A2 (en) * | 1989-04-20 | 1990-10-24 | Ajinomoto Co., Inc. | Process for producing L-ornithine by fermentation |
US5591613A (en) * | 1990-07-02 | 1997-01-07 | Degussa Aktiengesellschaft | Method for the preparation of D-arginine and L-ornithine |
-
2009
- 2009-09-17 CN CN2009101764929A patent/CN101691555B/zh not_active Expired - Fee Related
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0393708A2 (en) * | 1989-04-20 | 1990-10-24 | Ajinomoto Co., Inc. | Process for producing L-ornithine by fermentation |
US5591613A (en) * | 1990-07-02 | 1997-01-07 | Degussa Aktiengesellschaft | Method for the preparation of D-arginine and L-ornithine |
Non-Patent Citations (2)
Title |
---|
李环等.L-鸟氨酸的混合菌种发酵研究.《工业微生物》.2006,第36卷(第4期),37-42. * |
潘韵等.微生物发酵法生产L-鸟氨酸.《生物技术通讯》.2000,第11卷(第1期),61-64. * |
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