CN101668771B - 单链抗体的基于序列的工程改造和最优化 - Google Patents

单链抗体的基于序列的工程改造和最优化 Download PDF

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CN101668771B
CN101668771B CN2008800079463A CN200880007946A CN101668771B CN 101668771 B CN101668771 B CN 101668771B CN 2008800079463 A CN2008800079463 A CN 2008800079463A CN 200880007946 A CN200880007946 A CN 200880007946A CN 101668771 B CN101668771 B CN 101668771B
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amino acid
scfv
database
site
residue
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CN101668771A (zh
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D·厄驰
L·博拉斯
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Novartis AG
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Asba Technology Ag
Esbatech An Alcon Biomedical Research Unit LLC
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/24—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
    • C07K16/241—Tumor Necrosis Factors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C07K2317/565—Complementarity determining region [CDR]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C07K2317/567—Framework region [FR]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
    • C07K2317/622—Single chain antibody (scFv)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/90—Immunoglobulins specific features characterized by (pharmaco)kinetic aspects or by stability of the immunoglobulin
    • C07K2317/94—Stability, e.g. half-life, pH, temperature or enzyme-resistance
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B20/00—ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
CN2008800079463A 2007-03-12 2008-03-12 单链抗体的基于序列的工程改造和最优化 Active CN101668771B (zh)

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US90636507P 2007-03-12 2007-03-12
US60/906,365 2007-03-12
PCT/EP2008/001958 WO2008110348A1 (en) 2007-03-12 2008-03-12 Sequence based engineering and optimization of single chain antibodies

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CN101668771B true CN101668771B (zh) 2013-08-21

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US (2) US8280711B2 (https=)
EP (2) EP3202786A3 (https=)
JP (1) JP5622397B2 (https=)
CN (2) CN101668771B (https=)
AU (1) AU2008226067B2 (https=)
CA (1) CA2680564C (https=)
IL (1) IL200710A (https=)
IN (1) IN2009DN05758A (https=)
NZ (1) NZ579594A (https=)
RU (2) RU2009137582A (https=)
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EP2164961B1 (en) 2007-06-25 2015-01-07 ESBATech, an Alcon Biomedical Research Unit LLC Sequence based engineering and optimization of single chain antibodies
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Also Published As

Publication number Publication date
WO2008110348A1 (en) 2008-09-18
US20130053259A1 (en) 2013-02-28
EP3202786A2 (en) 2017-08-09
CN103275216B (zh) 2015-05-06
HK1138602A1 (en) 2010-08-27
EP3202786A3 (en) 2017-10-11
RU2009137582A (ru) 2011-04-20
CA2680564C (en) 2018-05-01
EP2118138A1 (en) 2009-11-18
RU2014127308A (ru) 2016-02-10
IL200710A (en) 2014-07-31
AU2008226067B2 (en) 2012-11-08
AU2008226067A1 (en) 2008-09-18
US9938336B2 (en) 2018-04-10
US8280711B2 (en) 2012-10-02
IN2009DN05758A (https=) 2015-07-24
US20100137150A1 (en) 2010-06-03
CN103275216A (zh) 2013-09-04
JP2010523077A (ja) 2010-07-15
NZ579594A (en) 2012-03-30
IL200710A0 (en) 2010-05-17
CA2680564A1 (en) 2008-09-18
CN101668771A (zh) 2010-03-10
JP5622397B2 (ja) 2014-11-12

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