CN101664007B - Sinonouacula hybridization breeding method - Google Patents
Sinonouacula hybridization breeding method Download PDFInfo
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- CN101664007B CN101664007B CN2009101529257A CN200910152925A CN101664007B CN 101664007 B CN101664007 B CN 101664007B CN 2009101529257 A CN2009101529257 A CN 2009101529257A CN 200910152925 A CN200910152925 A CN 200910152925A CN 101664007 B CN101664007 B CN 101664007B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The invention discloses a sinonouacula hybridization breeding method which comprises the following steps: selecting parent shellfish, promoting maturity, hastening parturition, inseminating, incubating, culturing larva and breeding to obtain hybridized sinonouacula which has high metabolism, higher life vitality and relatively high environmental adaption ability. Therefore, compared with Ninghai sinonouacula and Leging sinonouacula, the hybridized sinonouacula is a cultured species with bigger entity, quicker growth speed and higher economic efficiency. In the invention, boiled egg yolk is used for promoting maturity, low temperature and drying in shade are used for hastening parturition, larva is cultured under the conditions of reasonable culturing density, proper water temperature, salinity, pH value, lighting and the like, and the linear heredity breeding technology is used; the method in the invention is reasonable and scientific, the hybridized sinonouacula has relatively high ATP synthetase after testing, and conforms to the characteristics of hybridized species different from the Ninghai sinonouacula and Leging Ninghai sinonouacula.
Description
Technical field
The razor clam breeding that the present invention relates to hang is specifically related to a kind of razor clam cross breeding method of hanging.
Background technology
The razor clam (Sinonovacula constricta Lamark) of hanging, be commonly called as razor clam, dragonfly, extra large razor clam, belong to Mollusca (Mollusca), lamellibranchiata (LamellibranChia), different tooth subclass (Heterodonta), curtain clam order (Veneroida), razor clam section (Solcnidoe), the razor clam of hanging belong to (Sinonovacula).It is distributed in the coastal China in Western Pacific and Japanese, from China Liaoning to Guangdong Coastal distribution is arranged all, is called as one of four big cultivated shellfishes.The razor clam of hanging is nutritious, and delicious flavour is one of high protein, low fat, ocean health food low in calories.Present research for the razor clam of hanging mainly concentrates on several aspects such as ecological habit, nutrient component, cultural technique, genetic structure and biochemical hereditary feature analysis and carries out.And utilize the selection cross breeding not appear in the newspapers.
Summary of the invention
Technical problem to be solved by this invention provides a kind of razor clam cross breeding method of hanging, and the hybridization that obtains with this method is hung, and to have metabolism vigorous for razor clam, and vitality is higher, the relative advantage of higher of the ability that conforms.
The present invention solves the problems of the technologies described above the technical scheme that is adopted: a kind of razor clam cross breeding method of hanging comprises the steps:
1) close shellfish is selected: in October, the Ninghai of choosing for 2 ages razor clam and the Leqing razor clam of hanging of hanging is a hybrid strain, and described hybrid strain be individual healthy and strong, and water inlet pipe and water outlet pipe is flexible strong rapid, the individuality that gonad is full;
2) accelerating: after the hybrid strain washing and sterilizing, above-mentioned hybrid strain is gone into storage pond, holding pond support temporarily, the temporarily foster density of hybrid strain is 3500～4500/m
3Water body is is respectively thrown something and fed once with chrysophyceae and ripe yolk at storage pond, holding pond every day sooner or later, and every day, the feeding volume of chrysophyceae was 5～100,000/m
3Water body, every day, the feeding volume of ripe yolk was 5～10g/m
3Water body is supported the gonad maturity to hybrid strain temporarily; Utilize ripe yolk can allow hybrid strain do sth. in advance and the basic synchronization gonad maturity, fertilization rate is improved;
3) hasten parturition: with the hybrid strain of above-mentioned gonad maturity is that low temperature was hastened parturition 2～5 hours under 2～5 ℃ of conditions in temperature earlier, dries in the shade under normal temperature condition and hastens parturition 8 hours, hastens parturition with the flowing water flushing then, obtains sperm and egg cell;
4) fertilization and hatching: above-mentioned sperm and egg cell are mixed fertilization in 2: 1 number ratio, allow fertilized egg in the hatchery, inflate and hatch to D shape larva;
5) larvae cultivation: above-mentioned D shape larva pulled out to move on to 400 eye mesh screens cultivate in the pond inflation and cultivate to grow up to D shape larva and be juvenile mollusk, the cultivation density of cultivating D shape larva in the pond is 40～600,000/m
3The sea water specific gravity of cultivating in the pond is 1.01～1.03, pH value is 8.0～8.5, water temperature is 18～23 ℃, illumination is 1800～2300LmX, and change water once every day in the cultivation pond, and every day, quantity of exchanged water was 30～100%, sooner or later respectively throw something and feed once with chrysophyceae and flat algae every day in the cultivation pond, and every day, the feeding volume of chrysophyceae was 3～80,000/m
3Water body, every day, the feeding volume of flat algae was 0.5～20,000/m
3Water body;
6) breeding: choose individual big above-mentioned juvenile mollusk and carry out genetic breeding as lineal crossbreeding kind, the individual big filial generation of this crossbreeding kind is hybridizes the razor clam of hanging.Get hybridization razor clam, Ninghai the hang segment water pipe of razor clam of razor clam and Leqing of hanging of hanging, extract RNA, cDNA is synthesized in reverse transcription, carrying out PCR with kit and following primer detects: ATP synzyme primers F: 5 '-CAGGCTGCCA GATTTATTGG-3 ', R:5 '-GCTGCTTCAG GGATGGGTTT-3, beta-actin primers F: 5 '-AGTTGCCGCT CTTGTCGTGG-3 ', R:5 '-TGCTCTGGGCTTCATCTCCG-3, kit is provided by the precious biotechnology Co., Ltd of giving birth in Dalian, and primer is given birth to worker's biotechnology Co., Ltd by Shanghai and provided; Testing result, the hybridization razor clam of hanging is all higher more than 2 times than Ninghai the hang ATP synzyme of razor clam of razor clam, Leqing of hanging, illustrate that this kind is different from Ninghai razor clam, the Leqing razor clam of hanging of hanging,, show heterosis, hybrid vigor for the hybridization razor clam of hanging, this hybridization razor clam metabolism of hanging is vigorous, vitality is higher, and the ability that conforms is higher relatively, so fast growth, individual big, the business efficiency height of breed.
In the larvae cultivation step, when described D shape larvae cultivation after 5～8 days, in described cultivation pond, drop into soft husky mud, the injected volume of described soft husky mud is 2～3Kg/m
3Water body was changed described soft husky mud once every 3～5 days later on; Soft husky mud is better as the attaching substratum effect, and changing soft husky mud is in order to prevent that germ from growing.
Described soft husky mud is that the dried mud water of sea area beach soaks homogenate, and through 160～300 purpose silk cover filterings, the filtrate scalding is gone into the glar of pond precipitation after 24 hours, and filtered soft husky shale ground is good.
In the accelerating step, replace described ripe yolk with ripe soya-bean milk, every day, the feeding volume of ripe soya-bean milk was the ripe soya-bean milk/m of 5～10ml
3Water body.
Compared with prior art, the invention has the advantages that a kind of razor clam cross breeding method of hanging, by close shellfish selection, accelerating, hasten parturition, be fertilized and hatching, larvae cultivation and breeding, obtained the hybridization razor clam of hanging, this hybridization razor clam metabolism of hanging is vigorous, and vitality is higher, the ability that conforms is higher relatively, therefore its individuality is all bigger than Ninghai razor clam and the Leqing razor clam of hanging of hanging, and growth rate is also fast, is the breed variety of the higher business efficiency of a kind of tool.Breeding method of the present invention is with ripe yolk accelerating, hasten parturition with drying in the shade with low temperature, larvae cultivation with rational cultivation density, suitable conditions such as water temperature, salinity, pH value and illumination, lineal genetic breeding technology, method of the present invention is reasonable, science, it is higher to obtain hybridizing the hang relative quantity of its ATP synzyme after measured of razor clam, different with Ninghai razor clam and the Leqing razor clam of hanging of hanging, and meets the Hybrid feature.
Embodiment
Below in conjunction with embodiment the present invention is described in further detail.
Embodiment
A kind of razor clam cross breeding method of hanging at first in October, is chosen individually healthy and strong, and water inlet pipe and water outlet pipe is flexible rapidly strong, and the Ninghai in 2 ages that gonad is full razor clam and the Leqing razor clam of hanging of hanging is a hybrid strain; After the hybrid strain washing and sterilizing of choosing, drop into storage pond, holding pond and support every cubic metre of water body (m temporarily
3Water body) the temporarily foster density of hybrid strain is 3500～4500 in, sooner or later respectively throw something and feed once with chrysophyceae and ripe yolk every day at storage pond, holding pond, in every cubic metre of water body every day chrysophyceae feeding volume be 5～100,000, every day, the feeding volume of ripe yolk was 5～10g, supported the sexual gland basic synchronization maturation to hybrid strain temporarily; The hybrid strain of choosing gonad maturity is put into cryogenic box low temperature under 2～5 ℃ of (being generally 4 ℃) conditions earlier and hastened parturition 2～5 hours, when hastening parturition, low temperature to note observing, prevent that hybrid strain from freezing to death, under normal temperature condition, dry in the shade again after low temperature is hastened parturition and hasten parturition 8 hours, then hybrid strain is put into flowing water, allow flowing water wash, after the sexual gland of hybrid strain is subjected to stimulation by running water, sperm or egg cell have flowed out from sexual gland, collect sperm and egg cell, sperm and egg cell mix fertilization in 2: 1 number ratio, allow fertilized egg inflate in the hatchery and hatch to D shape larva; The D shape larva of hatchery pulled out to move on to 400 eye mesh screens cultivate in the pond inflation and cultivate to grow up to and be juvenile mollusk to D shape larva, the cultivation density of D shape larva is 40～600,000 in every cubic metre of water body in cultivation pond, the sea water specific gravity of cultivating in the pond is 1.01～1.03, pH value is 8.0～8.5, water temperature is 18～23 ℃, illumination is 1800～2300LmX, change water once every day in the cultivation pond, every day, quantity of exchanged water was 30～100%, sooner or later respectively throw something and feed once with chrysophyceae and flat algae every day in the cultivation pond, and every day, the feeding volume of chrysophyceae was 3～80,000/m
3Water body, every day, the feeding volume of flat algae was 0.5～20,000/m
3Water body when D shape larvae cultivation after 5～8 days, drops into soft husky mud through screening and high-temperature sterilization as substrate in cultivating the pond, the injected volume of soft husky mud is 2～3Kg/m
3Water body, moved later on every 3～5 days the pond change cultivate the pond substrate once, and seedling washed; After juvenile mollusk goes out the pond, transport storage pond, holding pond fast to, storage pond, holding pond changed water once in per 2～3 days, changed water 1/3～1/2 at every turn, and is thin according to pond liquid manure, the grouts supernatant etc. of in good time throwing something and feeding; Clear and Bright to Grain in Ear juvenile mollusk is evenly play razor clam farm in culture; Choose again wherein individual big, stalwartness, the razor clam of hanging in 2 ages that gonad is full is that lineal crossbreeding kind is carried out genetic breeding, also chooses wherein individual greatly in obtaining filial generation, healthy and strong juvenile mollusk is cultured with nature or artificial propagation and all can for the hybridization razor clam parent that hangs.
Ripe yolk also can replace with ripe soya-bean milk in the foregoing description, and every day, the feeding volume of ripe soya-bean milk was the ripe soya-bean milk/m of 5～10ml
3Water body.
Claims (4)
1. the razor clam cross breeding method of hanging is characterized in that comprising the steps:
1) close shellfish is selected: in October, the Ninghai of choosing for 2 ages razor clam and the Leqing razor clam of hanging of hanging is a hybrid strain, and described hybrid strain be individual healthy and strong, and water inlet pipe and water outlet pipe is flexible strong rapid, the individuality that gonad is full;
2) accelerating: after the hybrid strain washing and sterilizing, above-mentioned hybrid strain is gone into storage pond, holding pond support temporarily, the temporarily foster density of hybrid strain is 3500～4500/m
3Water body is is respectively thrown something and fed once with chrysophyceae and ripe yolk at storage pond, holding pond every day sooner or later, and every day, the feeding volume of chrysophyceae was 5～100,000/m
3Water body, every day, the feeding volume of ripe yolk was 5～10g/m
3Water body is supported the gonad maturity to hybrid strain temporarily;
3) hasten parturition: with the hybrid strain of above-mentioned gonad maturity is that low temperature was hastened parturition 2～5 hours under 2～5 ℃ of conditions in temperature earlier, dries in the shade under normal temperature condition and hastens parturition 8 hours, hastens parturition with the flowing water flushing then, obtains sperm and egg cell;
4) fertilization and hatching: above-mentioned sperm and egg cell are mixed fertilization in 2: 1 ratio, allow fertilized egg in the hatchery, inflate and hatch to D shape larva;
5) larvae cultivation: above-mentioned D shape larva pulled out to move on to 400 eye mesh screens cultivate in the pond inflation and cultivate to grow up to D shape larva and be juvenile mollusk, the cultivation density of cultivating D shape larva in the pond is 40～600,000/m
3Water body, the sea water specific gravity of cultivating in the pond is 1.01～1.03, pH value is 8.0～8.5, water temperature is 18～23 ℃, illumination 1800～2300LmX, change water once every day in the cultivation pond, and every day, quantity of exchanged water was 30～100%, sooner or later respectively throw something and feed once with chrysophyceae and flat algae every day in the cultivation pond, and every day, the feeding volume of chrysophyceae was 3～80,000/m
3Water body, every day, the feeding volume of flat algae was 0.5～20,000/m
3Water body;
6) breeding: choose individual big above-mentioned juvenile mollusk and carry out genetic breeding as the crossbreeding kind, the individual big filial generation of this crossbreeding kind is hybridizes the razor clam of hanging.
2. a kind of razor clam cross breeding method of hanging as claimed in claim 1 is characterized in that in the larvae cultivation step, when described D shape larvae cultivation after 5～8 days, drops into soft husky mud in described cultivation pond, and the injected volume of described soft husky mud is 2～3Kg/m
3Water body was changed described soft husky mud once every 3～5 days later on.
3. a kind of razor clam cross breeding method of hanging as claimed in claim 2 is characterized in that described soft husky mud is that the dried mud water of sea area beach soaks homogenate, and through 160～300 purpose silk cover filterings, the filtrate scalding is gone into the glar of pond precipitation after 24 hours.
4. a kind of razor clam cross breeding method of hanging as claimed in claim 1 is characterized in that in the accelerating step, replaces described ripe yolk with ripe soya-bean milk, and every day, the feeding volume of ripe soya-bean milk was 5～10ml/m
3Water body.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009101529257A CN101664007B (en) | 2009-09-21 | 2009-09-21 | Sinonouacula hybridization breeding method |
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| CN2009101529257A CN101664007B (en) | 2009-09-21 | 2009-09-21 | Sinonouacula hybridization breeding method |
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| CN101664007B true CN101664007B (en) | 2011-07-06 |
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101984795A (en) * | 2010-11-10 | 2011-03-16 | 谢绍河 | High-efficiency seawater pearl culture method |
| CN102100194A (en) * | 2010-12-02 | 2011-06-22 | 象山红升水产养殖有限公司 | Fry rearing method for sinonovacula constricta |
| CN103548726B (en) * | 2013-10-23 | 2015-07-22 | 上海海洋大学 | Establishment and stock breeding method for sinonovacula constricta families |
| CN111838035A (en) * | 2019-09-30 | 2020-10-30 | 浙江万里学院 | Method for screening sinonovacula constricta with strong salt tolerance by using osmotic pressure |
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2009
- 2009-09-21 CN CN2009101529257A patent/CN101664007B/en not_active Expired - Fee Related
Non-Patent Citations (4)
| Title |
|---|
| 侯和要等.大竹蛏人工繁殖技术研究.《齐鲁渔业》.2004,第21卷(第06期), * |
| 柴雪良等.小荚蛏人工育苗试验.《海洋科学》.2007,第31卷(第09期), * |
| 欧俊新.缢蛏室内育苗亲贝的选择和催产方法.《齐鲁渔业》.2002,第19卷(第12期), * |
| 郑汉丰等.三角帆蚌与池蝶蚌杂交繁育初试.《渔业现代化》.2007,(第02期), * |
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