CN101715743B - Method for culturing low-salt resistant juvenile stichopus - Google Patents
Method for culturing low-salt resistant juvenile stichopus Download PDFInfo
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- CN101715743B CN101715743B CN2009102207547A CN200910220754A CN101715743B CN 101715743 B CN101715743 B CN 101715743B CN 2009102207547 A CN2009102207547 A CN 2009102207547A CN 200910220754 A CN200910220754 A CN 200910220754A CN 101715743 B CN101715743 B CN 101715743B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The invention discloses a method for culturing the low-salt resistant juvenile stichopus of stable character, which can enlarge the stichopus culturing range and improve the stichopus culturing yield. The method for culturing the low-salt resistant juvenile stichopus comprises the following steps: collecting stichopus which has the weight of at least 300g and is cultured in the culturing water with the salinity of 22-28 for at least 1.5 year as parent stichopus; temporally culturing and demonstrating the parent stichopus in a temporary culturing pond of low salinity for 25 days; promoting the parent stichopus to spawn and fertilize in the shade with running water, controlling the temperature of the fertilized egg hatching water at 20-23 DEG C, the salinity of the fertilized egg hatching water at 22 and the fertilized egg hatching density at 10 in per milliliter of fertilized egg hatching water and inflating a little air; when the stichopus embryos developing into the initial larvae, culturing the initial larvae in different culturing ponds; when the initial larvae developing into the doliolarias, feeding the attaching base and changing 1/2 water once every day until each juvenile stichopus having the weight of 1g.
Description
Technical field:
The present invention relates to a kind of breeding method of juvenile stichopus, the breeding method of the low-salt resistant juvenile stichopus of especially a kind ofly enlarge the apostichopus japonicus culture scope, improve apostichopus japonicus culture output, proterties is stable.
Background technology:
Stichopus japonicus just is subject to people's favor since ancient times because having higher edibility and medical value, along with the increase to the stichopus japonicus demand, adopts and catches also gradually increase of intensity, causes China's stichopus japonicus stock number sharply to descend.At present, the artificial breeding of stichopus japonicus and cultivation scale just constantly enlarge, but because stichopus japonicus is marine animal, existing juvenile stichopus is relatively poor to the tolerance of salinity fluctuation and less salt, the salinity of breeding water reduces suddenly or lasting Low-salinity (below 24), and surviving and growing of stichopus japonicus will be had a strong impact on.As be located in the coastal area of rivers entrance owing to be subjected to the impact of rivers runoff, in arriving rainy season, the pond seawater salinity is just relatively on the low side, therefore the survival rate of stichopus japonicus, output, meat and nutrient component etc. all are affected, and have just limited the coastal area large-scale promotion that apostichopus japonicus culture is located in the rivers entrance.
Summary of the invention:
The present invention is in order to solve the existing above-mentioned technical problem of prior art, the breeding method of the low-salt resistant juvenile stichopus of provide a kind of and enlarge the apostichopus japonicus culture scope, improve apostichopus japonicus culture output, proterties is stable.
Technical solution of the present invention is: a kind of breeding method of low-salt resistant juvenile stichopus is characterized in that carrying out as follows:
A. before mating season, be collected in salinity and be in 22～28 the cultivation waters cultivation at least 1.5 years and body weight and be at least the stichopus japonicus of 300g as parent participation;
B. parent participation is placed storage pond, holding pond to support 25 days temporarily, storage pond, holding pond water is that the well water through precipitation mixes with nature seawater, and the range of decrease is 0.5 to salinity every day from 30, remains unchanged after being down to 22, storage pond, holding pond water pH value 7.8～8.5, dissolved oxygen be 16 ℃ of 5mg/L, temperature at least, support temporarily 25/m of density
2, the Articial bait making of throwing something and feeding during supporting temporarily;
C. adopting dries in the shade impels parent participation to lay eggs with flowing water, and the water temperature of laying eggs is 20 ℃, salinity 22; Adopt the siphon method to wash ovum with 300 mesh sieve tulles, the concentration of sperm is on a microscopic field face of ovum at least 3～5, and per stirring half an hour once;
D. the hatching water temperature of fertilized egg is 20 ℃～23 ℃, salinity 22, inflation, and hatching density is 10/mL;
E. work as embryo development to the microtia initial stage, select method to carry out seed selection with dragging, and be divided into the density of 0.2～0.3/ml and respectively cultivate the pond, cultivate 22 ℃ of pond water temperatures, salinity 22, the unit cell algae food of throwing something and feeding, feeding volume is 10,000 cells/ml, the middle bait young 20,000 cells/ml, the large bait young 30,000 cells/ml, quantity of exchanged water is the little bait young 1/4, the middle bait young 1/3, the large bait young 1/2 enters doliolarias stage input adherance;
F. enter the young ginseng Articial bait making of throwing something and feeding after the stage, change water every day 1 time, change water 1/2 at every turn, cultivate to the stichopus japonicus specification and on average reach the 1g/ head.
Bait feeding in the described b step is front 20 days of culture period.
After the described f step with seed according to a conventional method in that described salinity numerical value subtracts in 1 the cultivation waters and cultivates than a step, repeat a～f step 3～4 times when cultivating 1.5 years, every salinity numerical value that repeats once in described a, b, c, d, the e step all subtracts 1.
The present invention selects less salt is had the stichopus japonicus of certain adaptive capacity as parent participation, utilize the method for colony's seed selection to select breeding, cultivate anti-less salt (19～20) cultivating condition, juvenile stichopus that proterties is stable, can enlarge the apostichopus japonicus culture scope, improve the cultured output of stichopus japonicus, satisfy people to the demand of stichopus japonicus.
Embodiment:
A. be collected in salinity and be in 22～28 the cultivation waters cultivation at least 1.5 years and body weight and be at least the stichopus japonicus of 300g as parent participation;
B. parent participation is placed storage pond, holding pond to support 25 days temporarily, storage pond, holding pond water is that the well water through precipitation mixes with nature seawater, and the range of decrease is 0.5 to salinity every day from 30, remains unchanged after being down to 22, storage pond, holding pond water pH value 7.8～8.5, dissolved oxygen be 16 ℃ of 5mg/L, temperature at least, support temporarily 25/m of density
2, the Articial bait making (comprise sargassum thunbergii powder, sargassum powder, ooze and dried yeast powder etc., be purchased from " foreign biotechnology development centre is good in Dalian ") of throwing something and feeding during supporting temporarily;
Be preferably in the front 20 days bait of throwing something and feeding of culture period, namely before laying eggs, stopped to throw something and feed in 5 days, produce a large amount of ight soil polluted waters when preventing from laying eggs.
C. after supporting cultivation temporarily through 25 days reinforcement, selecting the individuality energetic, that gonad development good, individuality is large, appearance character is good as parent participation, adopting dries in the shade impels parent participation to lay eggs with flowing water, and the water temperature of laying eggs is 20 ℃, salinity 22; Adopt the siphon method to wash ovum with 300 mesh sieve tulles, the concentration of sperm is on a microscopic field face of ovum at least 3～5, and stir once per half an hour, makes fertilized egg mixing in the pond, should not stay dead angle and can not be to a direction rotation during stirring, center, idiosome rotation direction pond be assembled avoiding;
D. the hatching water temperature of fertilized egg is 20 ℃～23 ℃, salinity 22, is inflated to the water surface and has bubble to get final product, and hatching density is 10/mL;
E. work as embryo development to the microtia initial stage, whether the young that microscopy is hatched is normal, healthy and strong, select method to carry out seed selection with dragging, and be divided into the density of 0.25/ml and respectively cultivate the pond, cultivate 22 ℃ of pond water temperatures, salinity 22, the unit cell algae food of throwing something and feeding, feeding volume is 10,000 cells/ml, the middle bait young 20,000 cells/ml, the large bait young 30,000 cells/ml, quantity of exchanged water is the little bait young 1/4, the middle bait young 1/3, the large bait young 1/2;
F. enter doliolarias stage input adherance, adherance is the transparent polyethylene corrugated plating, polyethylene corrugated plate gauge lattice are 35cm*40cm, conspire to create with rope and to hang, every corrugated plating spacing is 5-8cm, whenever hangs 10 of string corrugated platings, change water every day 1 time, change water 1/2, the Articial bait making of throwing something and feeding is cultivated to the stichopus japonicus specification and is on average reached the 1g/ head at every turn.
The juvenile stichopus of cultivating can be rendered to less salt pond (namely the salinity numerical value than parent participation waters of living in subtracts 1) and cultivate according to a conventional method.Cultivate through repeatedly repeating, every final salinity numerical value that repeats once to cultivate its reinforced cultivating subtracts 1, can cultivate can be in 19～20 left and right sides salinity environment normal growth, and growth rate is higher than the stichopus japonicus colony that cultivates in salinity 28～35 environment.
Experimental result:
Contrast groups is the stichopus japonicus colony that cultivates in salinity 28～35 environment.
1. the fertilization rate in embodiment of the invention floating larva stage, incubation rate, survival rate and control group contrast see Table 1.
Table 1
The embodiment of the invention and Normal group stichopus japonicus be fully fertilization all, fertilization rate reached to 95%; Incubation rate reaches 68.3% incubation rate 62.7% a little more than control group; Survival rate reaches 38.5%, also is higher than 30.2% of control group.
2. the contrast of embodiment of the invention larval growth body weight and control group sees Table 2.
The embodiment of the invention young and the growing state in one monthly age of control group, two monthly ages, three monthly ages, age in April, age in May, age in June, age in July, age in August and age in September are measured, and its growth data sees Table 2:
Table 2
As can be seen from Table 2, the body weight of the embodiment of the invention is identical in the control group body weight value of a monthly age and two monthly ages and the same period, since the body weight value in age in three monthly ages to September all greater than the control group body weight value of the same period.
3. the contrast of embodiment of the invention larva survive ratio and control group sees Table 3.
Table 3
As can be seen from Table 3, the seed survival rate of the embodiment of the invention all is higher than the control group survival rate of the same period.
Claims (2)
1. the breeding method of a low-salt resistant juvenile stichopus is characterized in that carrying out as follows:
A. before mating season, be collected in salinity and be in 22～28 the cultivation waters cultivation at least 1.5 years and body weight and be at least the stichopus japonicus of 300g as parent participation;
B. parent participation is placed storage pond, holding pond to support 25 days temporarily, storage pond, holding pond water is that the well water through precipitation mixes with nature seawater, and the range of decrease is 0.5 to salinity every day from 30, remains unchanged after being down to 22, storage pond, holding pond water pH value 7.8～8.5, dissolved oxygen be 16 ℃ of 5mg/L, temperature at least, support temporarily 25/m of density
2, the Articial bait making of throwing something and feeding during supporting temporarily;
C. adopting dries in the shade impels parent participation to lay eggs with flowing water, and the water temperature of laying eggs is 20 ℃, salinity 22; Adopt the siphon method to wash ovum with 300 mesh sieve tulles, the concentration of sperm is on a microscopic field face of ovum at least 3～5, and per stirring half an hour once;
D. the hatching water temperature of fertilized egg is 20 ℃～23 ℃, salinity 22, inflation, and hatching density is 10/mL;
E. work as embryo development to the microtia initial stage, select method to carry out seed selection with dragging, and be divided into the density of 0.2～0.3/ml and respectively cultivate the pond, cultivate 22 ℃ of pond water temperatures, salinity 22, the unit cell algae food of throwing something and feeding, feeding volume is 10,000 cells/ml, the middle bait young 20,000 cells/ml, the large bait young 30,000 cells/ml, quantity of exchanged water is the little bait young 1/4, the middle bait young 1/3, the large bait young 1/2 enters doliolarias stage input adherance;
F. enter the young ginseng Articial bait making of throwing something and feeding after the stage, change water every day 1 time, change water 1/2 at every turn, cultivate to the stichopus japonicus specification and on average reach the 1g/ head.
2. the breeding method of low-salt resistant juvenile stichopus according to claim 1, it is characterized in that: after the described f step with seed according to a conventional method in that described salinity numerical value subtracts in 1 the cultivation waters and cultivates than a step, repetition a～f step 3 when cultivating 1.5 years～4 times, every salinity numerical value that repeats once in described a, b, c, d, the e step all subtracts 1.
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| CN2009102207547A CN101715743B (en) | 2009-12-15 | 2009-12-15 | Method for culturing low-salt resistant juvenile stichopus |
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| CN101715743B true CN101715743B (en) | 2013-01-16 |
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Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102100196B (en) * | 2010-12-20 | 2014-03-26 | 山东海益宝水产股份有限公司 | Method for breading sea cucumber seedlings |
| CN104957055B (en) * | 2015-06-04 | 2017-07-07 | 山东省海洋生物研究院 | A kind of selection of low-salt resistant juvenile stichopus |
| CN106538432A (en) * | 2016-11-06 | 2017-03-29 | 大连宝发海珍品有限公司 | A kind of resistance to less salt Stichopus japonicuss breeding method |
| CN106688986A (en) * | 2017-03-24 | 2017-05-24 | 青岛农业大学 | Indoor domestication method of California sea cucumber |
| CN107006409B (en) * | 2017-04-14 | 2019-03-08 | 山东省海洋生物研究院 | A kind of selection of the degeneration-resistant stichopus japonicus new lines of fast-growing |
| CN114342843A (en) * | 2022-01-26 | 2022-04-15 | 广东天海参威科技开发有限公司 | Low-salt-resistant sea cucumber cultivation method |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101156557A (en) * | 2007-11-22 | 2008-04-09 | 李成林 | deep well booth holothurian culture method |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101156557A (en) * | 2007-11-22 | 2008-04-09 | 李成林 | deep well booth holothurian culture method |
Non-Patent Citations (3)
| Title |
|---|
| 吕伟志 等.刺参生存盐度下限的探讨.《齐鲁渔业》.2006,第23卷(第5期),16. * |
| 王国利 等.温度与盐度对刺参(Apostichopus japonicus)生长的影响.《山东科学》.2007,第20卷(第3期),6-9. * |
| 肖培华 等.刺参对较低盐度的适应试验.《齐鲁渔业》.2004,第21卷(第6期),20－21. * |
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Address after: Heishijiao street Shahekou Dalian District 116000 Liaoning province No. 52 Patentee after: Dalian Ocean University Address before: Heishijiao street Shahekou Dalian District 116000 Liaoning province No. 52 Patentee before: Dalian Fisheries University |
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