Summary of the invention
Technical problem to be solved by this invention provides a kind of seed germination rate, the total shape elecampane vegetable drug that is produced on a large scale and artificial cultivation method of tiller root thereof of improving.
For addressing the above problem, the artificial cultivation method of a kind of total shape elecampane vegetable drug of the present invention, form by artificial raise seedling method and seedling cultivation method two parts, it is characterized in that: described artificial raise seedling method is meant that using organic base fertilizer after level land (1) ploughs, and carries out the whole ground of box whole ground or little furrow again; (2) total shape elecampane seed of wild or artificial production is removed fine hair,, reject immature seed by wind filter or the seed selection of hydraulic flotation method; (3) seed is put into Gibberellins solution immersion back and eluriated, again vernalization 6~8d under 15~25 ℃ of temperature with clear water; (4) seeds treated is carried out sowing in the field; After described seedling cultivation method is meant that the annual seedling that 1. will adopt described artificial raise seedling method to breed is gathered, select the rotten change of nothing, no mildew, undamaged seedling to grow up according to root and carry out classification in 25cm, 15~25cm, the following Three Estate of 15cm; 2. soak with liquor potassic permanganate or carbendazim solution, use flushing with clean water again; 3. seedling graduation the carrying out field-transplanting after will handling.
The box precipitation that refers in the described step (1) less than the 500mm area wholely, and employing 1~5m is wide, the dark 10~15cm of case carries out deeply.
Little furrow in the described step (1) refer to precipitation greater than the 550mm area wholely, adopt that 1~5m is wide to carry out.
Gibberellins solution concentration in the described step (3) is 100~150ppm.
The liquor potassic permanganate concentration of described step in 2. is 3~5 ‰, and carbendazim solution is with the solution after 500~800 times of the carbendazim solution dilutions of concentration 50%.
A kind of artificial cultivation method of total shape elecampane plant tiller root, it is characterized in that: after this method is at first gathered total shape elecampane tiller root of wild or artificial production, select the rotten change of nothing, no mildew, undamaged rhizome undercut to be slit into several according to keeping 1~2 of terminal bud; Soak with liquor potassic permanganate or carbendazim solution then, use flushing with clean water again, and be tiled in the container according to layer of fine sand one deck tiller root; Tiller root after will handling at last carries out field-transplanting; Wherein liquor potassic permanganate concentration is 3~5 ‰, and carbendazim solution is with the solution after 500~800 times of the carbendazim solution of concentration 50% dilutions.
The present invention compared with prior art has the following advantages:
1, the present invention handles total shape elecampane seed with the method that chemistry combines by physics, and seed germination rate is brought up to more than 95%; Mode by the seedling raising and transplanting field planting, can produce the total shape elecampane of the high-quality vegetable drug seedling that meets national traditional Chinese medicine quality of production management regulation (being called for short GAP) and Pharmacopoeia of the People's Republic of China requirement, and the experiment proved that total alkaloid content is similar in wild and tame total shape elecampane.
Different regions wild with the total shape elecampane of artificial cultivation in alantolactone content
Sample source |
The place of production |
Alantolactone content (mg/g) |
Isoalantolactone average content (mg/g) |
Wild |
Qinghai is controlled for a long time |
??27.53 |
??28.36 |
Wild |
Nagqu |
??27.81 |
??26.92 |
Wild |
The Maqu, Gansu |
??27.95 |
??28.05 |
Artificial cultivation |
Qinghai is logical greatly |
??28.13 |
??28.08 |
2, when adopting the artificial cultivation method of total shape elecampane plant tiller root disclosed in this invention; can produce stable yield, quality controllable total shape elecampane plant seed fast; produce total shape elecampane vegetable drug product for scale, large tracts of land high quality seed is provided, can satisfy foundation and meet the total shape elecampane medicinal material production demonstration base of national GAP standard and the requirement of high quality seed production base.
3, the present invention is easy to implement; can be applied in the total shape elecampane of height above sea level 1000~2500m medicinal material original producton location; when recovering total shape elecampane vegetable drug resource, also can realize protecting the purpose of highlands ecotope and the total shape elecampane endangered plants herb resource of rescue.
Embodiment
The artificial cultivation method of 1 one kinds of total shape elecampane vegetable drugs of embodiment is made up of artificial raise seedling method and seedling cultivation method two parts.Wherein:
Artificial raise seedling method is meant: after level land (1), use the ferment fertilizer that Qinghai bounties agrotechnique Co., Ltd produces by 3000kg/ mu, this organic base fertilizer is that specialized factory utilizes ox, animal wastes such as sheep become thoroughly decomposed by fermentation and add the fertilizer that biological rhzomorph is produced, its content of organic matter 〉=30%, quick-acting nitrogen, phosphorus, potassium content is respectively greater than 28%, 27% and 18%, and carry out 30cm and plough with interior shallow-layer, during fertilizer placed into the soil, it was wide to carry out 1~5m again, the box whole ground (precipitation is less than the 500mm area) that the dark 10~15cm of case is dark or to carry out 1~5m wide, the whole ground of little furrow (precipitation is greater than the 550mm area) that length is decided on landform; (2) with total shape elecampane seed of wild or artificial production by rubbing removal fine hair, utilize the seed selection of wind filter or hydraulic flotation method again, reject immature seed; (3) seed is immersed in the 100ppm Gibberellins solution (be called for short GA solution) behind the 24h, eluriates three times with clear water; Seeds treated is placed on 10~25 ℃ of indoor vernalization 8d; (4) the total shape elecampane plant seed after will handling carries out sowing in the field in mid-April in spring, and seeding method adopts the 50cm line-spacing to carry out bar and is sowed at height above sea level 1000~2500m irrigated land, and seeding quantity is 500~600g/ mu, sowing depth 5~8cm.
The seedling cultivation method is meant: 1. the annual seedling that artificial raise seedling method is bred selects the rotten change of nothing, no mildew, undamaged seedling to grow up according to root and carry out classification in 25cm, 15~25cm, the following Three Estate of 15cm after gathering at the beginning of 4 months next year; 2. soak with 3 ‰ liquor potassic permanganates, use flushing with clean water again; 3. the seedling graduation after will handling is according to 50~60 * 50~60cm seeding row spacing, and seed level 5~8cm carries out field-transplanting.
The artificial cultivation method of 2 one kinds of total shape elecampane vegetable drugs of embodiment is made up of artificial raise seedling method and seedling cultivation method two parts.Wherein:
Artificial raise seedling method is meant: after level land (1), use the ferment fertilizer that Qinghai bounties agrotechnique Co., Ltd produces by 3000kg/ mu, and carry out 30cm and plough with interior shallow-layer, during fertilizer placed into the soil, carry out again that 5m is wide, the box whole ground (precipitation is less than the 500mm area) of the dark 10~15cm of case or carry out the whole ground of little furrow (precipitation is greater than the 550mm area) that 1~5m is wide, length is decided on landform; (2) total shape elecampane seed of wild or artificial production is removed fine hair,, reject immature seed by wind filter or the seed selection of hydraulic flotation method; (3) seed is immersed in the 150ppm GA solution behind the 24h, eluriates three times with clear water; Seeds treated is placed on 15~25 ℃ of indoor or incubator vernalization 7~8d, seed germination rate is reached more than 95%; (4) the total shape elecampane plant seed after will handling carries out mid or late October in autumn or mid-April spring in next year sowing in the field then, seeding method adopts the 50cm line-spacing to carry out bar and is sowed at height above sea level 1000~2500m dry farming arable land (mean annual precipitation is greater than area more than the 500mm), seeding quantity is 500~600g/ mu, sowing depth 5~8cm after planting keeps soil moisture.
The seedling cultivation method is with embodiment 1.Wherein seedling is soaked with 4 ‰ liquor potassic permanganates.
The artificial cultivation method of 3 one kinds of total shape elecampane vegetable drugs of embodiment is made up of artificial raise seedling method and seedling cultivation method two parts.Wherein:
Artificial raise seedling method is meant: after level land (1), use the ferment fertilizer that Qinghai bounties agrotechnique Co., Ltd produces by 3000kg/ mu, and carry out 30cm and plough with interior shallow-layer, during fertilizer placed into the soil, carry out again that 5m is wide, the box whole ground (precipitation is less than the 500mm area) of the dark 10~15cm of case or carry out the whole ground of little furrow (precipitation is greater than the 550mm area) that 1~5m is wide, length is decided on landform; (2) with total shape elecampane seed of wild or artificial production by rubbing removal fine hair, utilize the seed selection of wind filter or hydraulic flotation method again, reject immature seed; (3) seed is immersed in the 130ppm GA solution behind the 24h, eluriates three times with clear water; Seeds treated is placed on 15~25 ℃ of indoor vernalization 7~8d, seed germination rate is reached more than 95%; (4) the total shape elecampane plant seed after will handling is in that mid or late October in autumn is carried out sowing in the field then, seeding method adopts the 50cm line-spacing to carry out bar and is sowed at height above sea level 1000~2500m irrigated land, seeding quantity is 500~600g/ mu, sowing depth 5~8cm, after planting the straw mat with straw or wheat straw establishment is tiled in ground, remove to hide straw mat at the beginning of 4 months coming year, can improve seed germination rate and promote the sprouting in advance of seed, increase total shape elecampane cycle 7~10d that grows.
The seedling cultivation method is with embodiment 1.Wherein seedling is soaked with 5 ‰ liquor potassic permanganates.
The artificial cultivation method of 4 one kinds of total shape elecampane vegetable drugs of embodiment is made up of artificial raise seedling method and seedling cultivation method two parts.Wherein:
Artificial raise seedling method is meant: after level land (1), use the ferment fertilizer that Qinghai bounties agrotechnique Co., Ltd produces by 3000kg/ mu, and carry out 30cm and plough with interior shallow-layer, during fertilizer placed into the soil, carry out again that 5m is wide, the box whole ground (precipitation is less than the 500mm area) of the dark 10~15cm of case or carry out the whole ground of little furrow (precipitation is greater than the 550mm area) that 1~5m is wide, length is decided on landform; (2) total shape elecampane seed of wild or artificial production is removed fine hair,, reject immature seed by wind filter or the seed selection of hydraulic flotation method; (3) seed is immersed in the 150ppm GA solution behind the 24h, eluriates three times with clear water; Seeds treated is placed on 10~25 ℃ of indoor storage 7~8d of cryopreservation, seed germination rate is reached more than 95%; (4) the total shape elecampane plant seed after will handling carries out mid or late October in autumn or mid-April spring in next year sowing in the field then, seeding method adopts the 50cm line-spacing to carry out bar and is sowed at height above sea level 1000~2500m dry farming arable land (mean annual precipitation is greater than area more than the 500mm), seeding quantity is 500~600g/ mu, sowing depth 5~8cm after planting keeps soil moisture.
The seedling cultivation method is with embodiment 1.Wherein use the carbendazim solution after the carbendazim solution of concentration 50% dilutes 500 times to soak to seedling.
The artificial cultivation method of 5 one kinds of total shape elecampane vegetable drugs of embodiment is made up of artificial raise seedling method and seedling cultivation method two parts.Wherein:
Artificial raise seedling method is meant: after level land (1), use the ferment fertilizer that Qinghai bounties agrotechnique Co., Ltd produces by 3000kg/ mu, and carry out 30cm and plough with interior shallow-layer, during fertilizer placed into the soil, carry out again that 5m is wide, the box whole ground (precipitation is less than the 500mm area) of the dark 10~15cm of case or carry out the whole ground of little furrow (precipitation is greater than the 550mm area) that 1~5m is wide, length is decided on landform; (2) total shape elecampane seed of wild or artificial production is removed fine hair,, reject immature seed by wind filter or the seed selection of hydraulic flotation method; (3) seed is immersed in the 150ppm GA solution behind the 24h, eluriates three times with clear water; Seeds treated is placed on 15~25 ℃ of indoor or incubator vernalization 7~8d, seed germination rate is reached more than 95%; (4) the total shape elecampane plant seed after will handling carries out mid or late October in autumn or mid-April spring in next year sowing in the field then, seeding method adopts the 50cm line-spacing to carry out bar and is sowed at height above sea level 1000~2500m dry farming arable land (mean annual precipitation is greater than area more than the 500mm), seeding quantity is 500~600g/ mu, sowing depth 5~8cm after planting keeps soil moisture.
The seedling cultivation method is with embodiment 1.Wherein use the carbendazim solution after the carbendazim solution of concentration 50% dilutes 700 times to soak to seedling.
The artificial cultivation method of 6 one kinds of total shape elecampane vegetable drugs of embodiment is made up of artificial raise seedling method and seedling cultivation method two parts.Wherein:
Artificial raise seedling method is meant: after level land (1), use the ferment fertilizer that Qinghai bounties agrotechnique Co., Ltd produces by 3000kg/ mu, and carry out 30cm and plough with interior shallow-layer, during fertilizer placed into the soil, carry out again that 5m is wide, the box whole ground (precipitation is less than the 500mm area) of the dark 10~15cm of case or carry out the whole ground of little furrow (precipitation is greater than the 550mm area) that 1~5m is wide, length is decided on landform; (2) with total shape elecampane seed of wild or artificial production by rubbing removal fine hair, utilize the seed selection of wind filter or hydraulic flotation method again, reject immature seed; (3) seed is immersed in the 130ppm GA solution behind the 24h, eluriates three times with clear water; Seeds treated is placed on 15~25 ℃ of indoor or incubator vernalization 7~8d, seed germination rate is reached more than 95%; (4) the total shape elecampane plant seed after will handling is in that mid or late October in autumn is carried out sowing in the field then, seeding method adopts the 50cm line-spacing to carry out bar and is sowed at height above sea level 1000~2500m irrigated land, seeding quantity is 500~600g/ mu, sowing depth 5~8cm, after planting the straw mat with straw or wheat straw establishment is tiled in ground, remove to hide straw mat at the beginning of 4 months coming year, can improve seed germination rate and promote the sprouting in advance of seed, increase total shape elecampane cycle 7~10d that grows.
The seedling cultivation method is with embodiment 1.Wherein use the carbendazim solution after the carbendazim solution of concentration 50% dilutes 800 times to soak to seedling.
The artificial cultivation method of 7 one kinds of total shape elecampane plant tiller roots of embodiment is meant: after at first total shape elecampane tiller root of wild or artificial production being gathered, select not have rotten change, no mildew, undamaged rhizome according to the principle that keeps 1~2 of terminal bud, undercut is slit into several; Soak 3~5min with 3 ‰ liquor potassic permanganates then, use flushing with clean water again 3 times, be placed on, and be tiled in the container and keep moistening, prevent the death of tiller root dehydration root system according to layer of fine sand one deck tiller root through in the good container of sterilization treatment, permeability; Tiller root after will handling at last is placed into storage in the low temperature darkroom of 0 ℃ of temperature or the cellar, mid or late October in autumn is carried out field-transplanting then, total shape elecampane plant tiller root after be about to handling is opened zanjon (ditch depth 50cm) according to 50~60 * 50~60cm seeding row spacing and is put and be sowed at height above sea level 1000~2500m irrigated land, seeding quantity 150~200kg/ mu, sowing depth 5~8cm (terminal bud earthing), after planting add a cover wheat straw or straw mat, remove hiding straw mat at the beginning of 4 months coming year can improve and cut tiller root survival rate of seedlings, tiller root survival rate of seedlings is reached more than 95%, increase total shape elecampane cycle 10~15d that grows simultaneously.
The artificial cultivation method of 8 one kinds of total shape elecampane plant tiller roots of embodiment is meant: after at first total shape elecampane tiller root of wild or artificial production being gathered, select not have rotten change, no mildew, undamaged rhizome according to the principle that keeps 1~2 of terminal bud, undercut is slit into several; Soak 3~5min with 5 ‰ liquor potassic permanganates then, use flushing with clean water again 3 times, be placed on, and be tiled in the container and keep moistening, prevent the death of tiller root dehydration root system according to layer of fine sand one deck tiller root through in the good container of sterilization treatment, permeability; Tiller root after will handling at last is placed into storage in the low temperature darkroom of 0 ℃ of temperature or the cellar, mid or late October in autumn is carried out field-transplanting then, total shape elecampane plant tiller root after be about to handling is opened zanjon (ditch depth 50cm) according to 50~60 * 50~60cm seeding row spacing and put and be sowed at height above sea level 1000~2500m dry farming plough (mean annual precipitation is greater than regional more than the 500mm), seeding quantity 150~200kg/ mu, sowing depth 5~8cm (terminal bud earthing), after planting add a cover wheat straw or straw mat, remove hiding straw mat at the beginning of 4 months coming year can improve and cut tiller root survival rate of seedlings, tiller root survival rate of seedlings is reached more than 95%, increase total shape elecampane cycle 10~15d that grows simultaneously.
The artificial cultivation method of 9 one kinds of total shape elecampane plant tiller roots of embodiment is meant: after at first total shape elecampane tiller root of wild or artificial production being gathered, select not have rotten change, no mildew, undamaged rhizome according to the principle that keeps 1~2 of terminal bud, undercut is slit into several; 500 times of liquid of carbendazim solution dilution with concentration 50% are used for soaking total shape elecampane tiller root then, use flushing with clean water 3 times behind 30~45min, be placed on through in the good container of sterilization treatment, permeability, and be tiled in the container and keep moistening according to layer of fine sand one deck tiller root, prevent the death of tiller root dehydration root system; Tiller root after will handling at last is placed into storage in the low temperature darkroom of 0 ℃ of temperature or the cellar, mid or late October in autumn is carried out field-transplanting then, total shape elecampane plant tiller root after be about to handling is opened zanjon (ditch depth 50cm) according to 50~60 * 50~60cm seeding row spacing and is put and be sowed at height above sea level 1000m~2500m water and water the arable land, seeding quantity 150~200kg/ mu, sowing depth 5~8cm (terminal bud earthing), after planting add a cover wheat straw or straw mat, remove hiding straw mat at the beginning of 4 months coming year can improve and cut tiller root survival rate of seedlings, tiller root survival rate of seedlings is reached more than 95%, increase total shape elecampane cycle 10~15d that grows simultaneously.
The artificial cultivation method of 10 1 kinds of total shape elecampane plant tiller roots of embodiment is meant: after at first total shape elecampane tiller root of wild or artificial production being gathered, select not have rotten change, no mildew, undamaged rhizome according to the principle that keeps 1~2 of terminal bud, undercut is slit into several; 800 times of liquid of carbendazim solution dilution with concentration 50% are used for soaking total shape elecampane tiller root then, use flushing with clean water 3 times behind 30~45min, be placed on through in the good container of sterilization treatment, permeability, and be tiled in the container and keep moistening according to layer of fine sand one deck tiller root, prevent the death of tiller root dehydration root system; Tiller root after will handling at last is placed into storage in the low temperature darkroom of 0 ℃ of temperature or the cellar, mid or late October in autumn is carried out field-transplanting then, total shape elecampane plant tiller root after be about to handling is opened zanjon (ditch depth 50cm) according to 50~60 * 50~60cm seeding row spacing and put and be sowed at height above sea level 1000~2500m dry farming plough (mean annual precipitation is greater than regional more than the 500mm), seeding quantity 150~200kg/ mu, sowing depth 5~8cm (terminal bud earthing), after planting add a cover wheat straw or straw mat, remove hiding straw mat at the beginning of 4 months coming year can improve and cut tiller root survival rate of seedlings, tiller root survival rate of seedlings is reached more than 95%, increase total shape elecampane cycle 10~15d that grows simultaneously.