CN101643589A - Extraction method of natural sunflower seed hull melanin - Google Patents
Extraction method of natural sunflower seed hull melanin Download PDFInfo
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- CN101643589A CN101643589A CN200810150679A CN200810150679A CN101643589A CN 101643589 A CN101643589 A CN 101643589A CN 200810150679 A CN200810150679 A CN 200810150679A CN 200810150679 A CN200810150679 A CN 200810150679A CN 101643589 A CN101643589 A CN 101643589A
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Abstract
The invention relates to an extraction method of natural sunflower seed hull melanin. The black solid powder sunflower seed hull melanin is extracted by the following steps: (1) crushing and screeningsunflower seed hulls; (2) processing with alkali; (3) filtering and deslagging; (4) processing filtrate with acid; (5) centrifuging; (6) repeating the steps (2), (3) and (4); (7) precipitating and washing; (8) centrifuging; and (9) drying. The method analyzes the concentration, the extraction temperature, the feed liquid ratio and the extraction time of an extraction solvent of melanin extractedfrom sunflower seed hulls by single factors and orthogonal experiments and confirms that the sunflower seed hull melanin is suitable for being extracted with 0.5 mol/L of NaOH at 25 DEG C for 1 hour until the feed liquid ratio is 1:25. A Folin-Ciocalteu (FC) colorimetric method is adopted to indirectly measure the content of the melanin in a crude extracting solution of the sunflower seed hull melanin, and the total polyphenol content of the sunflower seed hull melanin is about 0.48 percent (measured by gallic acid).
Description
Technical field
The invention belongs to agricultural byproducts comprehensive utilization field, relate to the melanic extraction of sunflower seeds processing byproduct sunflower seed skin.
Background technology
Sunflower has another name called Sunflower Receptacle, is feverfew, and all there is cultivation China various places.Sunflower Receptacle is medicine all over the body, and its seed, floral disc, cauline leaf, stem marrow, root, flower etc. all can be used as medicine, and secondly also has edible-type, oil type and dual-purpose type 3 classes.Oil with the sunflower of type after squeezing oil, remaining a large amount of seed Renhe seed skin in the grouts, the nutritive substance of the seed benevolence that remnants are a large amount of is processing and utilization further, contains a large amount of natural black pigment materials in the seed skin.
Melanochrome (Melanin) is prevalent in organic sphere; be the general name of complicated and diversified phenols of a class formation or indoles biomacromolecule pigment, can be organism structural strength or the biological injury of avoiding environment-stress such as ultraviolet ray, ionizing rays, heavy metal contamination, low temperature and high temperature of protection are provided.Melanochrome is not only a class bright protective agent, antiradiation agent, sequestrant, biological anti-oxidant and immunopotentiating agent, but also is a class bio-semiconductor material.Research in recent years also find melanochrome have antisnake venom, anticancer, suppress virus of AIDS and duplicate, treat effects such as parkinsonism.Therefore, melanochrome has a wide range of applications in fields such as medicine, makeup, food, electronics.At present, melanochrome both can carry out chemosynthesis by the oxidation of tyrosine or derivatives thereof, also can extract in the driven vegetable material, can also utilize microorganism synthetic.Comparatively speaking, the security of plant melanochrome is higher, and range of application is wider, but still lacks the raw material that melanin content is high and have value of exploiting and utilizing in activity in production.
Summary of the invention
The purpose of this invention is to provide a kind of extraction method of natural sunflower seed hull melanin.It is raw material that the oil that the Minqin, Gansu Province is originated in present method utilization extracts the cot by product that obtains behind the grease with sunflower seeds, a kind of Powdered natural black pigment that makes through operations such as aqueous solution vat liquor, acid treatment, oven dry.Determine that according to full wavelength scanner figure the pigment pyrocatechol type melanochrome in the sunflower seed skin is that polyphenols is converted into hydroxyquinone under the effect of polyphenoloxidase and phenolic hydroxyl group enzyme, and then polymerization formation.Sunflower seed skin melanochrome is soluble in alkaline solution, and water insoluble and common organic solvent when pH precipitates less than 3 the time, therefore adopts alkaline solution to extract melanochrome, handles making melanin deposition again with acid solution, to reach the purpose of extraction.
The melanic extracting method of a kind of sunflower seed skin the steps include: (1) sunflower seed skin crushing screening; (2) alkaline purification; (3) filter cleaner; (4) filtrate acid treatment; (5) centrifugal; (6) repeat (2), (3), (4); (7) washing of precipitate; (8) centrifugal; (9) drying.
Extract concentration that melanochrome in the sunflower seed skin extracts solvent, extract temperature, solid-liquid ratio and extraction time by single factor and orthogonal experiment analysis, determine that melanochrome is suitable for using 0.5mol/LNaOH at 25 ℃ of following ultrasonic extraction 1h in the sunflower seed skin, solid-liquid ratio extracts at 1: 25.Adopt melanic content in Folin-Ciocalteu (FC) the colorimetry indirect measurement sunflower seed skin melanochrome crude extract, sunflower seed skin melanochrome total polyphenols content is approximately about 0.48% (in gallic acid).
Sunflower seed skin is crossed 40 mesh sieves through pulverizing, and weighing is the sodium hydroxide solution of 0.5～1mol/L by solid-liquid ratio 1: 15～25 adding concentration, and is centrifugal at 25 ℃ of ultrasonic extraction 1h, gets supernatant liquor, adds the hydrochloric acid soln of isopyknic 2mol/L, leaves standstill, centrifugal after.Precipitation is used the 1mol/L dissolution of sodium hydroxide again, uses the 2mol/L salt Acid precipitation of equal volume again.This operation is 3～5 times repeatedly, and precipitation is washed 3～5 times with distillation more at last, and is centrifugal, is drying to obtain black solid powdered sunflower seed skin melanochrome.
Sunflower seed skin melanochrome is insoluble to acidic solution, is slightly soluble in water, is insoluble to common organic solvent, dissolves in alkaline solution; Its aqueous solution has maximum absorption band about 210nm under ultraviolet wavelength, increase with wavelength, and its absorption value descends.
Advantage of the present invention:
The melanochrome safety non-toxic that the present invention makes is done the colouring drinks agent, strong coloring force, good stability; Secondly melanochrome is rich in the potassium of amino acid and human body needs, phosphorus, and iron, selenium and other trace elements also contains protein, fat, carbohydrate, nicotinic acid, vitamin-E, vitamin A 5, carotene can be applicable to industries such as medicine, protective foods, makeup.
Present method adopts orthogonal experimental design, adopts ultrasonic extracting method, has determined the melanic optimum extraction process parameter of sunflower seed skin; Reflect melanin content indirectly in total phenol content (with gallic acid) in Folin-Ciocalteu (FC) the method mensuration melanochrome, method is simple.
Embodiment
Below, by specific embodiment the present invention is further described again:
Taking by weighing the raw material that 300g pulverizes, is the dissolution of sodium hydroxide of 1.0mol/L with 4500ml concentration, is that 100W, temperature are 25 ℃ of following ultrasonic extraction 1h at power; Centrifugal, get supernatant liquor and add isopyknic 2mol/L hydrochloric acid, leave standstill, centrifugal; Precipitation is used 1mol/L dissolution of sodium hydroxide, the 2mol/L salt Acid precipitation of equal volume again; This operation is 3～5 times repeatedly, and last precipitation is again with distillation washing 3～5 times, and is centrifugal, is drying to obtain sunflower melanochrome powder.
Claims (1)
1. extraction method of natural sunflower seed hull melanin.Its step is after the sunflower seed skin process is pulverized, to add sodium hydroxide solution, lixiviate or supersound extraction in water bath with thermostatic control, centrifuging adds isopyknic hydrochloric acid soln in the filtrate, leave standstill, after centrifugal, precipitation is used dissolution of sodium hydroxide again, isopyknic hydrochloric acid soln precipitation; Repeatable operation 3-5 time, precipitation is with distillation washing 3-5 time, centrifugal more at last, gets solid and is drying to obtain black solid powdered sunflower seed skin melanochrome at normal temperatures.
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| Application Number | Priority Date | Filing Date | Title |
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| CN200810150679A CN101643589A (en) | 2008-08-09 | 2008-08-09 | Extraction method of natural sunflower seed hull melanin |
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| CN200810150679A CN101643589A (en) | 2008-08-09 | 2008-08-09 | Extraction method of natural sunflower seed hull melanin |
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| CN101643589A true CN101643589A (en) | 2010-02-10 |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102229754A (en) * | 2011-05-12 | 2011-11-02 | 华中农业大学 | Method for extracting melanin from waste tremella cultivating material |
| EP3002043A1 (en) * | 2014-10-02 | 2016-04-06 | Biocyte | A method for preparing an olive extract with a melanin content greater than 5% |
| CN105017798B (en) * | 2014-07-08 | 2017-04-12 | 盐城师范学院 | Water-insoluble natural melanin extraction method |
| RU2637646C1 (en) * | 2017-01-09 | 2017-12-05 | Федеральное государственное бюджетное образовательное учреждение высшего образования "Волгоградский государственный технический университет" (ВолгГТУ) | Method for melanin production from sunflower husks |
| RU2643932C1 (en) * | 2017-06-06 | 2018-02-06 | Федеральное государственное бюджетное образовательное учреждение высшего образования "Волгоградский государственный технический университет" (ВолгГТУ) | Process for the preparation of melanin from sunflower pellets |
| RU2657499C1 (en) * | 2017-06-06 | 2018-06-14 | Федеральное государственное бюджетное образовательное учреждение высшего образования "Волгоградский государственный технический университет" (ВолгГТУ) | Process for obtaining melanin from sunflower husks |
| US20190150371A1 (en) * | 2016-06-13 | 2019-05-23 | Arturo Solis Herrera | Method of using melanin to fertilize soil |
| CN111778291A (en) * | 2020-06-23 | 2020-10-16 | 南京师范大学 | Method for increasing melanin production by fermenting or converting sunflower meal and sunflower shells by using microorganisms or enzymes |
| RU2780731C1 (en) * | 2021-06-02 | 2022-09-29 | федеральное государственное бюджетное образовательное учреждение высшего образования "Алтайский государственный технический университет им. И.И. Ползунова" (АлтГТУ) | Method for isolating melanin from buckwheat husks |
-
2008
- 2008-08-09 CN CN200810150679A patent/CN101643589A/en active Pending
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102229754A (en) * | 2011-05-12 | 2011-11-02 | 华中农业大学 | Method for extracting melanin from waste tremella cultivating material |
| CN105017798B (en) * | 2014-07-08 | 2017-04-12 | 盐城师范学院 | Water-insoluble natural melanin extraction method |
| EP3002043A1 (en) * | 2014-10-02 | 2016-04-06 | Biocyte | A method for preparing an olive extract with a melanin content greater than 5% |
| FR3026642A1 (en) * | 2014-10-02 | 2016-04-08 | Biocyte | PROCESS FOR THE PREPARATION OF AN EXTRACT OF OLIVE WITH A MELANINE CONTENT EXCEEDING 5% |
| US20190150371A1 (en) * | 2016-06-13 | 2019-05-23 | Arturo Solis Herrera | Method of using melanin to fertilize soil |
| RU2637646C1 (en) * | 2017-01-09 | 2017-12-05 | Федеральное государственное бюджетное образовательное учреждение высшего образования "Волгоградский государственный технический университет" (ВолгГТУ) | Method for melanin production from sunflower husks |
| RU2643932C1 (en) * | 2017-06-06 | 2018-02-06 | Федеральное государственное бюджетное образовательное учреждение высшего образования "Волгоградский государственный технический университет" (ВолгГТУ) | Process for the preparation of melanin from sunflower pellets |
| RU2657499C1 (en) * | 2017-06-06 | 2018-06-14 | Федеральное государственное бюджетное образовательное учреждение высшего образования "Волгоградский государственный технический университет" (ВолгГТУ) | Process for obtaining melanin from sunflower husks |
| CN111778291A (en) * | 2020-06-23 | 2020-10-16 | 南京师范大学 | Method for increasing melanin production by fermenting or converting sunflower meal and sunflower shells by using microorganisms or enzymes |
| RU2780731C1 (en) * | 2021-06-02 | 2022-09-29 | федеральное государственное бюджетное образовательное учреждение высшего образования "Алтайский государственный технический университет им. И.И. Ползунова" (АлтГТУ) | Method for isolating melanin from buckwheat husks |
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Application publication date: 20100210 |