CN101643589A - Extraction method of natural sunflower seed hull melanin - Google Patents
Extraction method of natural sunflower seed hull melanin Download PDFInfo
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- CN101643589A CN101643589A CN200810150679A CN200810150679A CN101643589A CN 101643589 A CN101643589 A CN 101643589A CN 200810150679 A CN200810150679 A CN 200810150679A CN 200810150679 A CN200810150679 A CN 200810150679A CN 101643589 A CN101643589 A CN 101643589A
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- sunflower seed
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- hull melanin
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- 235000020238 sunflower seed Nutrition 0.000 title claims abstract description 27
- XUMBMVFBXHLACL-UHFFFAOYSA-N Melanin Chemical compound O=C1C(=O)C(C2=CNC3=C(C(C(=O)C4=C32)=O)C)=C2C4=CNC2=C1C XUMBMVFBXHLACL-UHFFFAOYSA-N 0.000 title claims abstract description 26
- 238000000605 extraction Methods 0.000 title claims abstract description 13
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims abstract description 21
- 238000000034 method Methods 0.000 claims abstract description 8
- 238000001035 drying Methods 0.000 claims abstract description 5
- 239000007787 solid Substances 0.000 claims abstract description 4
- 238000005406 washing Methods 0.000 claims abstract description 4
- 239000000706 filtrate Substances 0.000 claims abstract description 3
- HVGQWHMSVYODLJ-GFCCVEGCSA-N melanochrome Natural products CC1(C)Oc2cc3OC(=CC(=O)c3c(O)c2C[C@H]1O)CO HVGQWHMSVYODLJ-GFCCVEGCSA-N 0.000 claims description 20
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 8
- 238000001556 precipitation Methods 0.000 claims description 7
- 238000004090 dissolution Methods 0.000 claims description 4
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- LNTHITQWFMADLM-UHFFFAOYSA-N gallic acid Chemical compound OC(=O)C1=CC(O)=C(O)C(O)=C1 LNTHITQWFMADLM-UHFFFAOYSA-N 0.000 abstract description 6
- 239000007788 liquid Substances 0.000 abstract description 5
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- 238000012545 processing Methods 0.000 abstract description 4
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- 239000006227 byproduct Substances 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 239000002994 raw material Substances 0.000 description 3
- 238000002137 ultrasound extraction Methods 0.000 description 3
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 238000003916 acid precipitation Methods 0.000 description 2
- 238000010306 acid treatment Methods 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
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- YCIMNLLNPGFGHC-UHFFFAOYSA-N catechol Chemical compound OC1=CC=CC=C1O YCIMNLLNPGFGHC-UHFFFAOYSA-N 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 238000004040 coloring Methods 0.000 description 2
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- 239000003960 organic solvent Substances 0.000 description 2
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- FPIPGXGPPPQFEQ-UHFFFAOYSA-N 13-cis retinol Natural products OCC=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-UHFFFAOYSA-N 0.000 description 1
- GPLIMIJPIZGPIF-UHFFFAOYSA-N 2-hydroxy-1,4-benzoquinone Chemical compound OC1=CC(=O)C=CC1=O GPLIMIJPIZGPIF-UHFFFAOYSA-N 0.000 description 1
- 208000030507 AIDS Diseases 0.000 description 1
- 102000030523 Catechol oxidase Human genes 0.000 description 1
- 108010031396 Catechol oxidase Proteins 0.000 description 1
- 235000008495 Chrysanthemum leucanthemum Nutrition 0.000 description 1
- 244000192528 Chrysanthemum parthenium Species 0.000 description 1
- 235000000604 Chrysanthemum parthenium Nutrition 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 1
- UPYKUZBSLRQECL-UKMVMLAPSA-N Lycopene Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1C(=C)CCCC1(C)C)C=CC=C(/C)C=CC2C(=C)CCCC2(C)C UPYKUZBSLRQECL-UKMVMLAPSA-N 0.000 description 1
- 208000027089 Parkinsonian disease Diseases 0.000 description 1
- 206010034010 Parkinsonism Diseases 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- FPIPGXGPPPQFEQ-BOOMUCAASA-N Vitamin A Natural products OC/C=C(/C)\C=C\C=C(\C)/C=C/C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-BOOMUCAASA-N 0.000 description 1
- 229930003427 Vitamin E Natural products 0.000 description 1
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- FPIPGXGPPPQFEQ-OVSJKPMPSA-N all-trans-retinol Chemical compound OC\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-OVSJKPMPSA-N 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
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- 150000001720 carbohydrates Chemical class 0.000 description 1
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- 150000001746 carotenes Chemical class 0.000 description 1
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- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 description 1
- 239000004519 grease Substances 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 230000002434 immunopotentiative effect Effects 0.000 description 1
- 150000002475 indoles Chemical class 0.000 description 1
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- 229910052742 iron Inorganic materials 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
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- 229960003512 nicotinic acid Drugs 0.000 description 1
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- 150000002989 phenols Chemical class 0.000 description 1
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- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1
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- NCYCYZXNIZJOKI-UHFFFAOYSA-N vitamin A aldehyde Natural products O=CC=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C NCYCYZXNIZJOKI-UHFFFAOYSA-N 0.000 description 1
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- Cosmetics (AREA)
Abstract
The invention relates to an extraction method of natural sunflower seed hull melanin. The black solid powder sunflower seed hull melanin is extracted by the following steps: (1) crushing and screeningsunflower seed hulls; (2) processing with alkali; (3) filtering and deslagging; (4) processing filtrate with acid; (5) centrifuging; (6) repeating the steps (2), (3) and (4); (7) precipitating and washing; (8) centrifuging; and (9) drying. The method analyzes the concentration, the extraction temperature, the feed liquid ratio and the extraction time of an extraction solvent of melanin extractedfrom sunflower seed hulls by single factors and orthogonal experiments and confirms that the sunflower seed hull melanin is suitable for being extracted with 0.5 mol/L of NaOH at 25 DEG C for 1 hour until the feed liquid ratio is 1:25. A Folin-Ciocalteu (FC) colorimetric method is adopted to indirectly measure the content of the melanin in a crude extracting solution of the sunflower seed hull melanin, and the total polyphenol content of the sunflower seed hull melanin is about 0.48 percent (measured by gallic acid).
Description
Technical field
The invention belongs to agricultural byproducts comprehensive utilization field, relate to the melanic extraction of sunflower seeds processing byproduct sunflower seed skin.
Background technology
Sunflower has another name called Sunflower Receptacle, is feverfew, and all there is cultivation China various places.Sunflower Receptacle is medicine all over the body, and its seed, floral disc, cauline leaf, stem marrow, root, flower etc. all can be used as medicine, and secondly also has edible-type, oil type and dual-purpose type 3 classes.Oil with the sunflower of type after squeezing oil, remaining a large amount of seed Renhe seed skin in the grouts, the nutritive substance of the seed benevolence that remnants are a large amount of is processing and utilization further, contains a large amount of natural black pigment materials in the seed skin.
Melanochrome (Melanin) is prevalent in organic sphere; be the general name of complicated and diversified phenols of a class formation or indoles biomacromolecule pigment, can be organism structural strength or the biological injury of avoiding environment-stress such as ultraviolet ray, ionizing rays, heavy metal contamination, low temperature and high temperature of protection are provided.Melanochrome is not only a class bright protective agent, antiradiation agent, sequestrant, biological anti-oxidant and immunopotentiating agent, but also is a class bio-semiconductor material.Research in recent years also find melanochrome have antisnake venom, anticancer, suppress virus of AIDS and duplicate, treat effects such as parkinsonism.Therefore, melanochrome has a wide range of applications in fields such as medicine, makeup, food, electronics.At present, melanochrome both can carry out chemosynthesis by the oxidation of tyrosine or derivatives thereof, also can extract in the driven vegetable material, can also utilize microorganism synthetic.Comparatively speaking, the security of plant melanochrome is higher, and range of application is wider, but still lacks the raw material that melanin content is high and have value of exploiting and utilizing in activity in production.
Summary of the invention
The purpose of this invention is to provide a kind of extraction method of natural sunflower seed hull melanin.It is raw material that the oil that the Minqin, Gansu Province is originated in present method utilization extracts the cot by product that obtains behind the grease with sunflower seeds, a kind of Powdered natural black pigment that makes through operations such as aqueous solution vat liquor, acid treatment, oven dry.Determine that according to full wavelength scanner figure the pigment pyrocatechol type melanochrome in the sunflower seed skin is that polyphenols is converted into hydroxyquinone under the effect of polyphenoloxidase and phenolic hydroxyl group enzyme, and then polymerization formation.Sunflower seed skin melanochrome is soluble in alkaline solution, and water insoluble and common organic solvent when pH precipitates less than 3 the time, therefore adopts alkaline solution to extract melanochrome, handles making melanin deposition again with acid solution, to reach the purpose of extraction.
The melanic extracting method of a kind of sunflower seed skin the steps include: (1) sunflower seed skin crushing screening; (2) alkaline purification; (3) filter cleaner; (4) filtrate acid treatment; (5) centrifugal; (6) repeat (2), (3), (4); (7) washing of precipitate; (8) centrifugal; (9) drying.
Extract concentration that melanochrome in the sunflower seed skin extracts solvent, extract temperature, solid-liquid ratio and extraction time by single factor and orthogonal experiment analysis, determine that melanochrome is suitable for using 0.5mol/LNaOH at 25 ℃ of following ultrasonic extraction 1h in the sunflower seed skin, solid-liquid ratio extracts at 1: 25.Adopt melanic content in Folin-Ciocalteu (FC) the colorimetry indirect measurement sunflower seed skin melanochrome crude extract, sunflower seed skin melanochrome total polyphenols content is approximately about 0.48% (in gallic acid).
Sunflower seed skin is crossed 40 mesh sieves through pulverizing, and weighing is the sodium hydroxide solution of 0.5～1mol/L by solid-liquid ratio 1: 15～25 adding concentration, and is centrifugal at 25 ℃ of ultrasonic extraction 1h, gets supernatant liquor, adds the hydrochloric acid soln of isopyknic 2mol/L, leaves standstill, centrifugal after.Precipitation is used the 1mol/L dissolution of sodium hydroxide again, uses the 2mol/L salt Acid precipitation of equal volume again.This operation is 3～5 times repeatedly, and precipitation is washed 3～5 times with distillation more at last, and is centrifugal, is drying to obtain black solid powdered sunflower seed skin melanochrome.
Sunflower seed skin melanochrome is insoluble to acidic solution, is slightly soluble in water, is insoluble to common organic solvent, dissolves in alkaline solution; Its aqueous solution has maximum absorption band about 210nm under ultraviolet wavelength, increase with wavelength, and its absorption value descends.
Advantage of the present invention:
The melanochrome safety non-toxic that the present invention makes is done the colouring drinks agent, strong coloring force, good stability; Secondly melanochrome is rich in the potassium of amino acid and human body needs, phosphorus, and iron, selenium and other trace elements also contains protein, fat, carbohydrate, nicotinic acid, vitamin-E, vitamin A 5, carotene can be applicable to industries such as medicine, protective foods, makeup.
Present method adopts orthogonal experimental design, adopts ultrasonic extracting method, has determined the melanic optimum extraction process parameter of sunflower seed skin; Reflect melanin content indirectly in total phenol content (with gallic acid) in Folin-Ciocalteu (FC) the method mensuration melanochrome, method is simple.
Embodiment
Below, by specific embodiment the present invention is further described again:
Taking by weighing the raw material that 300g pulverizes, is the dissolution of sodium hydroxide of 1.0mol/L with 4500ml concentration, is that 100W, temperature are 25 ℃ of following ultrasonic extraction 1h at power; Centrifugal, get supernatant liquor and add isopyknic 2mol/L hydrochloric acid, leave standstill, centrifugal; Precipitation is used 1mol/L dissolution of sodium hydroxide, the 2mol/L salt Acid precipitation of equal volume again; This operation is 3～5 times repeatedly, and last precipitation is again with distillation washing 3～5 times, and is centrifugal, is drying to obtain sunflower melanochrome powder.
Claims (1)
1. extraction method of natural sunflower seed hull melanin.Its step is after the sunflower seed skin process is pulverized, to add sodium hydroxide solution, lixiviate or supersound extraction in water bath with thermostatic control, centrifuging adds isopyknic hydrochloric acid soln in the filtrate, leave standstill, after centrifugal, precipitation is used dissolution of sodium hydroxide again, isopyknic hydrochloric acid soln precipitation; Repeatable operation 3-5 time, precipitation is with distillation washing 3-5 time, centrifugal more at last, gets solid and is drying to obtain black solid powdered sunflower seed skin melanochrome at normal temperatures.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200810150679A CN101643589A (en) | 2008-08-09 | 2008-08-09 | Extraction method of natural sunflower seed hull melanin |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200810150679A CN101643589A (en) | 2008-08-09 | 2008-08-09 | Extraction method of natural sunflower seed hull melanin |
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| Publication Number | Publication Date |
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| CN101643589A true CN101643589A (en) | 2010-02-10 |
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| CN200810150679A Pending CN101643589A (en) | 2008-08-09 | 2008-08-09 | Extraction method of natural sunflower seed hull melanin |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102229754A (en) * | 2011-05-12 | 2011-11-02 | 华中农业大学 | Method for extracting melanin from waste tremella cultivating material |
| EP3002043A1 (en) * | 2014-10-02 | 2016-04-06 | Biocyte | A method for preparing an olive extract with a melanin content greater than 5% |
| CN105017798B (en) * | 2014-07-08 | 2017-04-12 | 盐城师范学院 | Water-insoluble natural melanin extraction method |
| RU2637646C1 (en) * | 2017-01-09 | 2017-12-05 | Федеральное государственное бюджетное образовательное учреждение высшего образования "Волгоградский государственный технический университет" (ВолгГТУ) | Method for melanin production from sunflower husks |
| RU2643932C1 (en) * | 2017-06-06 | 2018-02-06 | Федеральное государственное бюджетное образовательное учреждение высшего образования "Волгоградский государственный технический университет" (ВолгГТУ) | Process for the preparation of melanin from sunflower pellets |
| RU2657499C1 (en) * | 2017-06-06 | 2018-06-14 | Федеральное государственное бюджетное образовательное учреждение высшего образования "Волгоградский государственный технический университет" (ВолгГТУ) | Process for obtaining melanin from sunflower husks |
| US20190150371A1 (en) * | 2016-06-13 | 2019-05-23 | Arturo Solis Herrera | Method of using melanin to fertilize soil |
| CN111778291A (en) * | 2020-06-23 | 2020-10-16 | 南京师范大学 | A kind of method that utilizes microorganism or enzyme to ferment or transform sunflower meal and sunflower shell to increase the production of melanin |
| RU2780731C1 (en) * | 2021-06-02 | 2022-09-29 | федеральное государственное бюджетное образовательное учреждение высшего образования "Алтайский государственный технический университет им. И.И. Ползунова" (АлтГТУ) | Method for isolating melanin from buckwheat husks |
-
2008
- 2008-08-09 CN CN200810150679A patent/CN101643589A/en active Pending
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102229754A (en) * | 2011-05-12 | 2011-11-02 | 华中农业大学 | Method for extracting melanin from waste tremella cultivating material |
| CN105017798B (en) * | 2014-07-08 | 2017-04-12 | 盐城师范学院 | Water-insoluble natural melanin extraction method |
| EP3002043A1 (en) * | 2014-10-02 | 2016-04-06 | Biocyte | A method for preparing an olive extract with a melanin content greater than 5% |
| FR3026642A1 (en) * | 2014-10-02 | 2016-04-08 | Biocyte | PROCESS FOR THE PREPARATION OF AN EXTRACT OF OLIVE WITH A MELANINE CONTENT EXCEEDING 5% |
| US20190150371A1 (en) * | 2016-06-13 | 2019-05-23 | Arturo Solis Herrera | Method of using melanin to fertilize soil |
| RU2637646C1 (en) * | 2017-01-09 | 2017-12-05 | Федеральное государственное бюджетное образовательное учреждение высшего образования "Волгоградский государственный технический университет" (ВолгГТУ) | Method for melanin production from sunflower husks |
| RU2643932C1 (en) * | 2017-06-06 | 2018-02-06 | Федеральное государственное бюджетное образовательное учреждение высшего образования "Волгоградский государственный технический университет" (ВолгГТУ) | Process for the preparation of melanin from sunflower pellets |
| RU2657499C1 (en) * | 2017-06-06 | 2018-06-14 | Федеральное государственное бюджетное образовательное учреждение высшего образования "Волгоградский государственный технический университет" (ВолгГТУ) | Process for obtaining melanin from sunflower husks |
| CN111778291A (en) * | 2020-06-23 | 2020-10-16 | 南京师范大学 | A kind of method that utilizes microorganism or enzyme to ferment or transform sunflower meal and sunflower shell to increase the production of melanin |
| RU2780731C1 (en) * | 2021-06-02 | 2022-09-29 | федеральное государственное бюджетное образовательное учреждение высшего образования "Алтайский государственный технический университет им. И.И. Ползунова" (АлтГТУ) | Method for isolating melanin from buckwheat husks |
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Application publication date: 20100210 |