CN104211828B - A kind of Fructus Mori polysaccharide and preparation method thereof - Google Patents
A kind of Fructus Mori polysaccharide and preparation method thereof Download PDFInfo
- Publication number
- CN104211828B CN104211828B CN201410473365.6A CN201410473365A CN104211828B CN 104211828 B CN104211828 B CN 104211828B CN 201410473365 A CN201410473365 A CN 201410473365A CN 104211828 B CN104211828 B CN 104211828B
- Authority
- CN
- China
- Prior art keywords
- fructus mori
- polysaccharide
- preparation
- alkali
- rinse
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a kind of Fructus Mori polysaccharide and preparation method thereof.With commercially available Fructus Mori as raw material, use water to carry to put forward the mode combined with alkali and extract, water is the most first used to carry, obtain par-tial polysaccharide extracting solution, again filtering residue is carried out alkali to carry, obtain another part polysaccharide extraction liquid, above-mentioned two parts extracting solution is merged and is concentrated to give end product, farthest improve the yield of Fructus Mori polysaccharide.Meanwhile, the polysaccharide of the preparation method gained of Fructus Mori polysaccharide provided by the present invention can keep higher antioxidant activity.
Description
Technical field
The invention belongs to food processing technology field, relate to the preparation method of a kind of polysaccharide, particularly relate to one
The preparation method of the polysaccharide with Fructus Mori as raw material.
Background technology
Fructus Mori is the by-product of planting mulberry and breeding silkworm, as Chinese medicine material, has YIN nourishing of enriching blood, the effect of profit bath of promoting the production of body fluid.Wherein
Polysaccharide is one of maximally effective medicinal active ingredient of Fructus Mori.Research finds that Fructus Mori polysaccharide has antioxidation, blood sugar lowering, resists
Tumor, anti-inflammatory isoreactivity.It addition, Fructus Mori is as natural plants, it there is no negative interaction to human body.So,
The biological activity of exploitation Fructus Mori, and it is applied to health food, there is wide market.
There is the Study on Extraction Method report much about Fructus Mori polysaccharide in recent years.Hot water extraction is utilized to extract Fructus Mori polysaccharide,
Investigated various extracting conditions on the impact of Fructus Mori polysaccharide extract rate (Wei Zhaojun etc., the optimization of Extraction of Polysaccharide from Mulberry Fruit,
Food Science, 2007,28 (11): 261-264).Use water extraction to be aided with ultrasonic technology from Fructus Mori, extract polysaccharide
Method (Jiang Yan, Xinjiang black mulberry polysaccharide ultrasonic extraction process is preferred, and China brewages, 2009,3:138-140).
Utilize cellulase to extract Fructus Mori polysaccharide from Fructus Mori, used by experiment of single factor and L9 (34) orthogonal experiment studying enzyme
Amount, enzymolysis time, hydrolysis temperature on the impact of Fructus Mori polysaccharide extract rate (Liu Xiaolu etc., Enzymatic Extraction Fructus Mori polysaccharide
Technical study, Chinese Wild plant resources, 2012,31 (1): 30-32).Fructus Mori polysaccharide has radical scavenging activity
Power and effect (Zhang Wenna etc., Fructus Mori polysaccharide anti-oxidative activity research, the Agriculture of Anhui section of suppression Linoleic Acid Oxidation activity
Skill, 2012,40 (1): 156-157).The extraction of polysaccharide or preparation method affect its antioxidant activity (Wang Yanli etc.,
The ultrasound parameter impact on Rhizoma Atractylodis Macrocephalae polysaccharide anti-oxidative activity, biological processing [J], 2012,10 (1): 7-12；?
Beautiful rosy clouds etc., the Different Extraction Method impact on Fuscoporia obliqua polysaccharide antioxidant activity, Agriculture of Anhui science [J], 2012,
40(10):5870-5872)。
Therefore, those skilled in the art is devoted to develop the preparation method of a kind of efficient Fructus Mori polysaccharide,
While improving polysaccharide yield, keep the high antioxidant of polysaccharide.
Summary of the invention
Because the drawbacks described above of prior art, how the technical problem to be solved is at preparation Fructus Mori antioxygen
During changing polysaccharide, both improved the yield of polysaccharide, kept again the high antioxidant of polysaccharide.
For achieving the above object, the invention provides the preparation method of a kind of Fructus Mori polysaccharide, especially by following
Technical scheme realizes:
1) mixing: Fructus Mori remove impurity cleaned, adds distilled water, smashes at a high speed with polisher, obtains mixed liquor；
2) water carries: under conditions of heating-up temperature is 80-100 DEG C, to step 1) in mixed liquor carry out magnetic agitation
2-3 hour, then by mixed liquor through filter-cloth filtering, obtain filtrate and be Aqueous extracts, filtering residue taking-up is carried out alkali and carries；
3) alkali carries: in step 2) filtering residue in add NaOH solution, under conditions of heating-up temperature is 80-100 DEG C
Carry out magnetic agitation, through filter-cloth filtering after 2-3 hour, obtain filtrate and be alkali extract；
4) dialysis: to step 3) alkali extract in add hydrochloric acid, regulation solution ph is to 7, and then dialysis is 24 little
Time；
5) concentrate: combining step 2) in Aqueous extracts and step 4) in dialysis after alkali extract, in heating-up temperature be
Concentrate under the conditions of 80-100 DEG C, obtain concentrated solution；
6) precipitation: in step 5) concentrated solution in add ethanol, stand overnight, to remove protein；
7) rinse: by step 6) in stand overnight after concentrated solution be centrifuged, remove the supernatant, organic with low boiling
Solvent rinse precipitates；
8) it being dried: by step 7) precipitation after rinse dries up, and obtains Fructus Mori polysaccharide.
Preferably, step 1) in Fructus Mori be commercially available Fructus Mori, its water content by weight is 25%；Add distilled water
20-40 times that weight is Fructus Mori weight；Polisher used is household type or use for laboratory type.
Preferably, step 2) and step 3) in filter cloth used be 200 mesh or more than.
Preferably, step 3) the middle concentration adding NaOH solution is 0.5mol/L, and its weight added is filtering residue
20-40 times of weight.
Preferably, step 4) in the hydrochloric acid that adds be the dilute hydrochloric acid solution after concentrated hydrochloric acid dilution, its preferably concentration is
6mol/L；The molecular cut off that bag filter specification is 3500Da used in dialysis procedure.
Preferably, step 5) concentration process carry out on the constant temperature heating device of adjustable temperature, finally give is dense
Contracting liquid amasss 1/20 amassed into condensate precursor.
Preferably, step 6) in add the amount of ethanol be to make the final volume percentage ratio of ethanol in mixed solution dense
Degree is more than 80%.
Preferably, step 7) in centrifugal rotating speed be 4000rpm；Low boiling point organic solvent used by rinse be acetone,
One or more in ethanol and ether.
Additionally, present invention also offers the Fructus Mori polysaccharide being prepared in aforementioned manners.
Present invention employs above technical scheme, have such advantages as and beneficial effect:
1, the invention provides the preparation method of a kind of Fructus Mori polysaccharide, compared to existing customary preparation methods,
The polysaccharide of gained has higher yield.
2, the Fructus Mori polysaccharide of gained of the present invention, compared to existing customary preparation methods, non-oxidizability is higher.
Below with reference to accompanying drawing, the design of the present invention and the technique effect of generation are described further, to be fully understood from
The purpose of the present invention, feature and effect.
Accompanying drawing explanation
Fig. 1 is two preferred embodiments yield comparison diagram with comparative example gained polysaccharide of the present invention.
Fig. 2 is two preferred embodiments non-oxidizability comparison diagram with comparative example gained polysaccharide of the present invention, wherein TE
For trolox equivalent.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is described in further detail, but embodiments of the present invention are not limited to this.
Embodiment 1
1) mixing: commercially available Fructus Mori remove impurity cleaned, adds the distilled water of 20 times of weight, smashes at a high speed with polisher,
Obtain mixed liquor；
2) water carries: under the conditions of heating-up temperature is 100 DEG C, to step 1) in mixed liquor carry out magnetic agitation, water carries
2 hours, then by mixed liquor filter-cloth filtering, obtain filtrate and be Aqueous extracts, filtering residue taking-up is carried out alkali and carries；
3) alkali carries: in step 2) filtering residue in add 20 times of weight NaOH solution (0.5mol/L), heating
Temperature carries out magnetic agitation under the conditions of being 100 DEG C, and alkali uses filter-cloth filtering after carrying 2 hours, obtains filtrate and is alkali extract；
4) dialysis: to step 3) alkali extract in add hydrochloric acid (6mol/L), regulation solution ph to 7, then
Dialyse 24 hours；
5) concentrate: combining step 2) in Aqueous extracts and step 4) in dialysis after alkali extract, in heating-up temperature be
It is concentrated into the 1/20 of original volume under the conditions of 80 DEG C, obtains concentrated solution；
6) precipitation: in step 5) concentrated solution in add ethanol so that the volume basis that after mix homogeneously, ethanol is final
Specific concentration is more than 80%, stands overnight；
7) rinse: by step 6) in the concentrated solution that stands overnight centrifugal after remove the supernatant, in succession by acetone and second
Ether rinse precipitates；
8) it being dried: by step 7) precipitation after rinse dries up, weighs, and carry out antioxidant activity evaluation.
Embodiment 2
1) mixing: commercially available Fructus Mori remove impurity cleaned, adds the distilled water of 40 times of weight, smashes at a high speed with polisher,
Obtain mixed liquor；
2) water carries: under the conditions of heating-up temperature is 80 DEG C, to step 1) in mixed liquor carry out magnetic agitation, water carries 3
Hour, then by mixed liquor filter-cloth filtering, obtain filtrate and be Aqueous extracts, filtering residue taking-up is carried out alkali and carries；
3) alkali carries: in step 2) filtering residue in add 40 times of weight NaOH solution (0.5mol/L), heating
Temperature carries out magnetic agitation under conditions of being 80 DEG C, and alkali uses filter-cloth filtering after carrying 3 hours, obtains filtrate and is alkali extract；
4) dialysis: to step 3) alkali extract in add hydrochloric acid (6mol/L), regulation solution ph to 7, then
Dialyse 24 hours；
5) concentrate: combining step 2) in Aqueous extracts and step 4) in dialysis after alkali extract, in heating-up temperature be
It is concentrated into the 1/20 of original volume under the conditions of 100 DEG C, obtains concentrated solution；
6) precipitation: in step 5) concentrated solution in add ethanol so that the volume basis that after mix homogeneously, ethanol is final
Specific concentration is more than 80%, stands overnight；
7) rinse: by step 6) in the concentrated solution that stands overnight centrifugal after remove the supernatant, use dehydrated alcohol rinse
Precipitate twice；
8) it being dried: by step 7) precipitation after rinse dries up, weighs, and carry out antioxidant activity evaluation.
Comparative example
1) mixing: commercially available Fructus Mori remove impurity cleaned, adds the distilled water of 20 times of weight, smashes at a high speed with polisher,
Obtain mixed liquor；
2) water carries for the first time: under conditions of heating-up temperature is 100 DEG C, to step 1) in mixed liquor carry out magnetic force
Stirring, water carries 3 hours, then by mixed liquor filter-cloth filtering, retains, the Aqueous extracts obtained by further for filtering residue water
Carry；
3) water carries for the second time: in step 2) filtering residue in add 20 times of weight distilled water, be 100 in heating-up temperature
Carrying out magnetic agitation under conditions of DEG C, water carries 3 hours, then with filter-cloth filtering, is retained by the Aqueous extracts obtained；
4) concentrate: combining step 2) and step 3) in Aqueous extracts, be concentrated under the conditions of heating-up temperature is 80 DEG C
The 1/20 of original volume, obtains concentrated solution；
5) precipitation: in step 4) concentrated solution in add ethanol, and make the final volume percent concentration of ethanol be more than
80%, stand overnight；
6) rinse: by step 5) in stand overnight after the concentrated solution centrifugal segregation supernatant, in succession with acetone and
Ether rinse precipitates；
7) it being dried: by step 6) precipitation after rinse dries up, weighs, and carry out antioxidant activity evaluation.
Evaluation experimental
Embodiment 1, embodiment 2 and comparative example gained Fructus Mori polysaccharide are carried out antioxidation ORAC evaluation respectively.
Concrete evaluation methodology is as follows: add 300 μ l water at 96 hole ELISA Plate (black) most peripherals, in measured hole successively
Add 25 μ l samples, the 4 × 10 of 150 μ l-6MM Fluress.ELISA Plate is put in microplate reader, at 37 DEG C
After preheating 30min, add 25 μ l AAPH and start fluorescent quenching reaction.Its empty group replaces with 25 μ l buffer
Sample, matched group replaces sample with the Trolox solution of 25 μ l variable concentrations.Sample parallel measures three times.Result with
Trolox content represents ORAC value as equivalent.Microplate reader measuring condition is provided that excitation wavelength 485nm,
Launch wavelength 528nm；After adding AAPH, amplitude 1mm, shakes 8s.After reaction starts, measure cycle-index
Being 120 times, cycle period is 1min.
Result with compare
As it is shown in figure 1, the polysaccharide yield of embodiment 1 is 2.85% (w/w), the polysaccharide yield of embodiment 2 is 3.01%
(w/w), and comparative example is 1.73% (w/w).As can be seen here, the system of Fructus Mori polysaccharide provided by the present invention
Preparation Method, compared to existing customary preparation methods, can improve the yield of Fructus Mori polysaccharide.
As in figure 2 it is shown, the ORAC value of embodiment 1 gained polysaccharide is 54.6 μm ol TE/mg, embodiment 2 institute is much
The ORAC value of sugar is 73.8 μm ol TE/mg, and the ORAC value of comparative example gained polysaccharide is 11.4 μm ol TE/mg.
I.e. embodiment 2 gained polysaccharide anti-oxidative activity is the highest, is secondly the antioxidant activity of embodiment 1 gained polysaccharide, and right
The antioxidant activity of ratio gained polysaccharide is minimum.As can be seen here, the preparation of Fructus Mori polysaccharide provided by the present invention
Method, compared to existing customary preparation methods, can improve the antioxidant activity of Fructus Mori polysaccharide.
The preferred embodiment of the present invention described in detail above.Should be appreciated that those of ordinary skill in the art without
Need creative work just can make many modifications and variations according to the design of the present invention.Therefore, all in the art
Technical staff the most on the basis of existing technology can by logical analysis, reasoning, or a limited experiment
With the technical scheme obtained, all should be in the protection domain being defined in the patent claims.
Claims (6)
1. the preparation method of a Fructus Mori polysaccharide, it is characterised in that comprise the following steps:
1) mixing: Fructus Mori remove impurity cleaned, adds distilled water, smashes at a high speed with polisher, obtains mixed liquor；
2) water carries: under conditions of heating-up temperature is 80-100 DEG C, to step 1) in mixed liquor carry out magnetic agitation
2-3 hour, then by mixed liquor through filter-cloth filtering, obtain filtrate and be Aqueous extracts, filtering residue taking-up is carried out alkali and carries；
3) alkali carries: in step 2) filtering residue in add NaOH solution, under conditions of heating-up temperature is 80-100 DEG C
Carry out magnetic agitation, through filter-cloth filtering after 2-3 hour, obtain filtrate and be alkali extract；Wherein, the described NaOH of addition
The concentration of solution is 0.5mol/L, and its weight ratio with described filtering residue is 20-40:1；
4) dialysis: to step 3) alkali extract in add hydrochloric acid, regulation solution ph is to 7, and then dialysis is 24 little
Time；Wherein, the molecular cut off that bag filter specification is 3500Da that described dialysis uses；
5) concentrate: combining step 2) in Aqueous extracts and step 4) in dialysis after alkali extract, in heating-up temperature be
Concentrate under the conditions of 80-100 DEG C, obtain concentrated solution；Wherein, the concentrated solution that described concentration finally gives is that condensate precursor amasss
1/20；
6) precipitation: in step 5) concentrated solution in add ethanol, stand overnight；
7) rinse: by step 6) in stand overnight after concentrated solution be centrifuged, remove the supernatant, organic with low boiling
Solvent rinse precipitates；
8) it being dried: by step 7) precipitation after rinse dries up, and obtains Fructus Mori polysaccharide.
The preparation method of Fructus Mori polysaccharide the most according to claim 1, it is characterised in that step 1) in
Fructus Mori by weight, its water content is 25%, and the weight ratio adding distilled water and described Fructus Mori is 20-40:1.
The preparation method of Fructus Mori polysaccharide the most according to claim 1, it is characterised in that step 2) and
Step 3) described in filter cloth be 200 mesh or more than.
The preparation method of Fructus Mori polysaccharide the most according to claim 1, it is characterised in that step 6) in
The amount of described addition ethanol is that the final volume percent concentration making ethanol in mixed solution is more than 80%.
The preparation method of Fructus Mori polysaccharide the most according to claim 1, it is characterised in that step 7) in
Described centrifugal rotating speed is 4000rpm.
The preparation method of Fructus Mori polysaccharide the most according to claim 1, it is characterised in that step 7) in
The low boiling point organic solvent that described rinse is used is one or more in acetone, ethanol and ether.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410473365.6A CN104211828B (en) | 2014-09-17 | 2014-09-17 | A kind of Fructus Mori polysaccharide and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410473365.6A CN104211828B (en) | 2014-09-17 | 2014-09-17 | A kind of Fructus Mori polysaccharide and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104211828A CN104211828A (en) | 2014-12-17 |
| CN104211828B true CN104211828B (en) | 2016-08-24 |
Family
ID=52093760
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410473365.6A Active CN104211828B (en) | 2014-09-17 | 2014-09-17 | A kind of Fructus Mori polysaccharide and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104211828B (en) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105147718A (en) * | 2015-07-22 | 2015-12-16 | 贵州师范大学 | Application of mulberry polysaccharide extract in preparing medicine or health product |
| CN104987431B (en) * | 2015-07-28 | 2017-03-08 | 华南理工大学 | A kind of Fructus Mori active polysaccharide and its extracting method |
| CN105175259B (en) * | 2015-08-20 | 2017-03-22 | 深圳大学 | Mulberry monomer compounds, preparation method and application thereof |
| CN110016084B (en) * | 2018-01-08 | 2021-05-28 | 中国科学院上海药物研究所 | Mulberry proteoglycan, preparation method and application thereof |
| CN110734501A (en) * | 2019-11-04 | 2020-01-31 | 山东圣源绿色食品科技有限公司 | Preparation method of mulberry extracts |
| CN111643517B (en) * | 2020-04-26 | 2022-11-01 | 贵州师范大学 | Application of mulberry polysaccharide derivative S-MFP-30 in preparation of antitumor drugs |
| CN111481564B (en) * | 2020-04-26 | 2022-11-01 | 贵州师范大学 | Application of mulberry polysaccharide MFP-90-2 in preparation of antitumor drugs |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103848921B (en) * | 2012-12-05 | 2016-02-10 | 中国科学院大连化学物理研究所 | A kind of black fruit lyceum alkali-extracted polysaccharide and Synthesis and applications thereof |
| CN103044566B (en) * | 2013-01-04 | 2015-07-01 | 合肥工业大学 | Method for preparing antioxidant polysaccharide from dictyophora indusiata aqueous extract residues |
-
2014
- 2014-09-17 CN CN201410473365.6A patent/CN104211828B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN104211828A (en) | 2014-12-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104211828B (en) | A kind of Fructus Mori polysaccharide and preparation method thereof | |
| CN101899174B (en) | Bletilla polysaccharide compound serving as cosmetic raw material and preparation method thereof | |
| CN104231103B (en) | Flammulina velutipes polysaccharide as well as preparation method and application thereof | |
| CN101367881B (en) | High-efficiency preparation method for glossy ganoderma polysaccharide | |
| CN106132390A (en) | The Melanin inhibitor being made up of D pantothenylol and the skin-lightening cosmetic containing this Melanin inhibitor | |
| CN103445159B (en) | A kind of enzymatic-process preparation method of full constituent ginseng juice | |
| CN103073648B (en) | A kind of method improving flammulina velutipes anti-oxidant activity | |
| CN106866834B (en) | A kind of method and its application for preparing the efficiently fucoidin of customization molecular weight | |
| CN104140471B (en) | A kind of method preparing Rhizoma Dioscoreae high-purity polysaccharide from yam food processing fent | |
| CN103059162B (en) | A kind of novel method of high efficiency extraction lentinan | |
| CN104072627B (en) | A kind of preparation method of Herba Typhonii gigantei polyoses extract | |
| CN110464682A (en) | A kind of peach gum composition, preparation method and application | |
| CN102146142A (en) | Method for preparing astragalus polysaccharides | |
| CN103931982A (en) | Method for preparing pollen nutrient solution by ultrasonic and pulse electric field | |
| CN102512353B (en) | Dictyophora indusiata water extract and preparation method and application thereof | |
| CN104211829B (en) | Preparation and application of white fungus functional polysaccharide | |
| CN105646728B (en) | A kind of honey comb polyoses extract and preparation method thereof | |
| CN104322857A (en) | Modified peony seed meal protein preparation method | |
| CN103073913A (en) | Method for comprehensively extracting various functional components in color wheat bran | |
| CN104262507B (en) | A kind of ultrasonic synergistic CDA enzyme is prepared the method for lobster shell shitosan | |
| CN102940688A (en) | Extraction method for polysaccharides and total flavonoids in fig leaves | |
| CN101407559B (en) | Method for rapidly preparing yeast beta-1,3-dextran | |
| CN104193846B (en) | Extract method and the application of polysaccharide thereof of polysaccharide in matrimony vine | |
| CN104059159B (en) | The processing method of Mierocrystalline cellulose Enzymatic Extraction Herba Hedyotidis Diffusae polysaccharide | |
| CN105639657A (en) | Method for extracting mineral elements in boxthorn leaves through enzymolysis |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |