CN103073913A - Method for comprehensively extracting various functional components in color wheat bran - Google Patents
Method for comprehensively extracting various functional components in color wheat bran Download PDFInfo
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- CN103073913A CN103073913A CN2013100260127A CN201310026012A CN103073913A CN 103073913 A CN103073913 A CN 103073913A CN 2013100260127 A CN2013100260127 A CN 2013100260127A CN 201310026012 A CN201310026012 A CN 201310026012A CN 103073913 A CN103073913 A CN 103073913A
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Abstract
The invention relates to a method for comprehensively extracting various functional components in color wheat bran. The method comprises the steps of extracting pigment in bran through an acidified ethanol method, washing residue till the residue is neutral, collecting starch washed out, leaching the neutral residue with hot water, so as to obtain water-soluble pentosan, performing zymohydrolysis to residue with amylase, so as to remove residual starch and obtain coarse dietary fiber, adding alkali liquor to the dietary fiber to leach the dietary fiber, so as to obtain mixed leaching liquor of water-insoluble pentosan and protein, adding Sevage reagent to the mixed leaching liquor, performing vibration centrifugation to separate protein precipitation from pentosan solution, allowing pentosan solution to be subjected to decoloration by activated carbon, then performing concentration and ethanol extraction to the pentosan solution, and obtaining water-insoluble pentosan after drying.
Description
(1) technical field
The present invention relates to a kind of comprehensive extractive technique of the several functions compositions such as pigment, starch, water-soluble piperylene, food fibre, protein, water unextractable pentosan in the color grain Testa Tritici, belong to the fields such as light industry, agricultural, foodstuffs industry.
(2) background technology
The difference of color grain wheat and common wheat maximum is to contain abundant pigment, mainly concentrates on the wheat bran part behind the abrasive dust.Except containing abundant pigment, also contain the nutritive ingredients such as starch, piperylene, protein, food fibre in the color grain Testa Tritici.At present, people only limit to the extraction of certain single component or two kinds of compositions for the extraction of nutritive ingredient in the Testa Tritici, have extracted behind the target component remaining residue mostly as feed, cause the waste with resource of losing of multiple nutritional components.Therefore, seek a kind of technology much more as far as possible nutritive ingredient in the wheat bran is extracted, can realize taking full advantage of of wheat bran, can improve its economic worth again, have very high using value and practice significance.
(3) summary of the invention
The objective of the invention is to research and develop a kind of with comprehensive complete set technologys of extracting of several functions composition such as the pigment in the color grain Testa Tritici, starch, water-soluble piperylene, food fibre, protein, water unextractable pentosans.
Its method is, extracts pigment in the wheat bran with the acidifying Ethanol Method first, again with washing residue to neutral, collect the starch that washes out, the reusable heat flooding is washed to neutral residue, gets water-soluble piperylene.Residual residue is carried out enzymolysis with amylase and is removed wherein residual starch, get thick food fibre, in food fibre, add the alkali lye lixiviate, obtain the mixing vat liquor of water unextractable pentosan and protein, add Sevage reagent in the vat liquor to mixing, shake centrifugally, isolate protein precipitation and piperylene solution.Piperylene solution with activated carbon decolorizing after concentrated alcohol analyse, obtain water unextractable pentosan after the drying.
(4) description of drawings
The several functions composition comprehensively extracts flow process in the color grain of Fig. 1 Testa Tritici
1 is color grain Testa Tritici among Fig. 1, the 2nd, and dehydrated alcohol, the 3rd, concentrated hydrochloric acid, the 4th, deionized water, the 5th, acidifying ethanolic soln, the 6th, water-bath, the 7th, centrifugal, the 8th, pigment extract, the 9th, rotary evaporation, the 10th, freeze-drying, the 11st, pigment, the 12nd, residue behind the extraction pigment, the 13rd, washing, the 14th, starch, the 15th, drainage residue, the 16th, water-soluble piperylene extracting solution, the 17th, leave standstill, the 18th, water-soluble piperylene, the 19th, extract the residue behind the water-soluble piperylene, the 20th, amylase, the 21st, thick food fibre, the 22nd, sodium hydroxide lye, the 23rd, piperylene and protein mixed extract, the 24th, trichloromethane, the 25th, propyl carbinol, the 26th, Sevage reagent, the 27th, protein, the 28th, water unextractable pentosan solution, the 29th, gac, the 30th, decolouring, the 31st, filter, the 32nd, water unextractable pentosan
(5) embodiment
1. pigment extracts
Get color grain Testa Tritici (1), with dehydrated alcohol (2), 37% concentrated hydrochloric acid (3), deionized water (4) be mixed with that pH is 1～3, alcohol concn is 30%～60% acidifying ethanolic soln (5), join in the wheat bran by solid-liquid ratio 1:10～1:30,50～80 ℃ of lower water-bath (6) 40～70min, centrifugal (7) get supernatant liquor, repetitive operation 2 times, united extraction gained supernatant liquor (8), rotary evaporation (9) is concentrated, and freeze-drying (10) gets wheat bran pigment (11), and yield is 14.5%.
2. the extraction of starch and water-soluble piperylene
Get the residue (12) that extracts behind the pigment, washing (13) is to neutral, collect and the oven dry washing lotion in starch (14), yield is 5%.The deionized water (4) that adds 10～30 times in wheat bran in the residue (15) after the washing, in 60～90 ℃ of lower water-bath (6) 40～90min, centrifugal (7) get supernatant liquor, repeat again to extract 1 time, merge gained supernatant liquor (16), add the dehydrated alcohol (2) of 4 times of volumes, 4 ℃ leave standstill (17) and spend the night, centrifugal (7) collecting precipitation, dry (10) namely get water-soluble piperylene (18), and yield is 1.4%.
3. the preparation of thick food fibre
Get and extract residue (19) behind the water-soluble piperylene, add deionized water (4), transferring pH is 4～7, the α-amylase (20) that adds wheat bran quality 0.5%～1%, 40～70 ℃ of lower water-bath (6) 40～70min, centrifugal (7) get residue, and dry (10) obtain thick food fibre (21), and yield is 48%
4, the extraction of protein and water unextractable pentosan
The NaOH solution (22) of preparation 0.05%～0.1%, 10～30 times by wheat bran (1) quality join in the thick food fibre, 80～100 ℃ of water-bath (6) 50～90min, and centrifugal (7) get supernatant liquor, repeat to extract 2 times, merging supernatant liquor is piperylene and protein mixed extract (23); Chloroform (24) is mixed with Sevage reagent (26) with propyl carbinol (25) with volume ratio 4:1, Sevage reagent is by adding the gained supernatant liquor with sample liquid volume ratio 1:1～1:3, shake centrifugal (7), repetitive operation is 1 time again, merging is precipitated as protein (27), and supernatant liquor is water unextractable pentosan solution (28); In water unextractable pentosan solution (28), add 3%～6% gac (29), 40～80 ℃ of lower water-baths (6) decolouring (30) 40～70min, funnel filters (31), add 4 times of volume dehydrated alcohols (2), 4 ℃ leave standstill (15) and spend the night, centrifugal (7) collecting precipitation, dry (10) namely get water unextractable pentosan (33), and yield is 7.3%.
The several functions compositions such as the pigment that the application aforesaid method obtains, starch, water-soluble piperylene, food fibre, protein, water unextractable pentosan are good foodstuff additive, and wherein pigment is natural colorant, has good oxidation-resistance; Water-soluble piperylene makes an addition in the bread can increase loaf volume; Food fibre, water unextractable pentosan all have preferably, and logical intestines moisten just effect; Starch can provide energy as polysaccharide; Protein contains the multiple amino acids of needed by human, has better nutritivity and is worth.These functional ingredient can be widely used in food-processing, healthcare products, medicine, biotechnology field, and applicability is strong, applied range, and good market outlook are arranged.
Claims (1)
1. the combined extraction method of the several functions compositions such as pigment, starch, water-soluble piperylene, food fibre, protein, water unextractable pentosan in the color grain Testa Tritici, it is characterized in that: first compound concentration is 30%～60% ethanolic soln, transferring pH is 1～3, join in the wheat bran 50～80 ℃ of lower water-bath 40～70min, centrifuging and taking supernatant liquor by solid-liquid ratio 1:10～1:30, repetitive operation is 2 times again, united extraction gained supernatant liquor, rotary evaporation is concentrated, and freeze-drying gets the wheat bran pigment; Residue behind the extraction pigment, be washed to neutrality, collect and dry the starch in the washing lotion, add the deionized water of 10～30 times in wheat bran in the residue after the washing, in 60～90 ℃ of lower water-bath 40～90min, the centrifugal supernatant liquor that gets repeats to extract 1 time again, merges the gained supernatant liquor, the dehydrated alcohol that adds 4 times of volumes, 4 ℃ of standing over night, centrifugal collecting precipitation is drying to obtain water-soluble piperylene; Get and extract residue behind the water-soluble piperylene, add deionized water, transferring pH is 4～7, adds the α-amylase of wheat bran quality 0.5%～1%, 40～70 ℃ of lower water-bath 40～70min, and the centrifuging and taking residue, drying obtains thick food fibre; Prepare again 0.05%～0.1% NaOH solution, 10～30 times by the wheat bran quality join in the thick food fibre, 80～100 ℃ of water-bath 50～90min, the centrifugal supernatant liquor that gets, repeat to extract 2 times, merging supernatant liquor is water unextractable pentosan and protein mixed extract; Chloroform and propyl carbinol are mixed with Sevage reagent with volume ratio 4:1, Sevage reagent by and sample liquid volume ratio 1:1～1:3 add the gained supernatant liquor, shake centrifugally, repetitive operation is 1 time again, merging is precipitated as protein, supernatant liquor is water unextractable pentosan solution; Add 3%～6% gac in the water unextractable pentosan solution, 40～80 ℃ of lower water-bath decolouring 40～70min, funnel filters, and adds 4 times of volume dehydrated alcohols, 4 ℃ of standing over night, centrifugal collecting precipitation is drying to obtain water unextractable pentosan.
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Cited By (6)
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| CN103613220A (en) * | 2013-11-21 | 2014-03-05 | 中国农业科学院农产品加工研究所 | Method for extracting multiple functional components from wheat starch processing wastewater |
| CN104264262A (en) * | 2014-09-01 | 2015-01-07 | 郸城财鑫糖业有限责任公司 | Method for extracting araboxylan and protein fiber from wheat bran |
| CN105001284A (en) * | 2015-07-08 | 2015-10-28 | 山西省农业科学院小麦研究所 | Anthocyanin extraction method for purple spring wheat bran |
| CN105010953A (en) * | 2015-06-02 | 2015-11-04 | 郑州轻工业学院 | Wheat bran dietary fiber and wheat bran small peptide cogeneration process |
| CN107048423A (en) * | 2017-03-31 | 2017-08-18 | 重庆师范大学 | A kind of method of the beautiful agaric dietary fiber of alkalinity extraction |
| CN113632876A (en) * | 2021-08-11 | 2021-11-12 | 山西大学 | Production process for adding artificial meat into colored wheat bran |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103613220A (en) * | 2013-11-21 | 2014-03-05 | 中国农业科学院农产品加工研究所 | Method for extracting multiple functional components from wheat starch processing wastewater |
| CN103613220B (en) * | 2013-11-21 | 2016-05-04 | 中国农业科学院农产品加工研究所 | A kind of method of extracting several functions component from wheaten starch processing waste water |
| CN104264262A (en) * | 2014-09-01 | 2015-01-07 | 郸城财鑫糖业有限责任公司 | Method for extracting araboxylan and protein fiber from wheat bran |
| CN104264262B (en) * | 2014-09-01 | 2016-02-03 | 郸城财鑫糖业有限责任公司 | A kind of method extracting araboxylan and protein fibre from wheat bran |
| CN105010953A (en) * | 2015-06-02 | 2015-11-04 | 郑州轻工业学院 | Wheat bran dietary fiber and wheat bran small peptide cogeneration process |
| CN105001284A (en) * | 2015-07-08 | 2015-10-28 | 山西省农业科学院小麦研究所 | Anthocyanin extraction method for purple spring wheat bran |
| CN105001284B (en) * | 2015-07-08 | 2017-12-15 | 山西省农业科学院小麦研究所 | A kind of purple spring wheat wheat bran anthocyanin extracting method |
| CN107048423A (en) * | 2017-03-31 | 2017-08-18 | 重庆师范大学 | A kind of method of the beautiful agaric dietary fiber of alkalinity extraction |
| CN107048423B (en) * | 2017-03-31 | 2020-06-19 | 重庆师范大学 | Method for extracting edible fungus dietary fiber by alkaline process |
| CN113632876A (en) * | 2021-08-11 | 2021-11-12 | 山西大学 | Production process for adding artificial meat into colored wheat bran |
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Application publication date: 20130501 |