CN102864671B - The reuse method of Rhizomatic traditional Chinese medicine slag and application - Google Patents
The reuse method of Rhizomatic traditional Chinese medicine slag and application Download PDFInfo
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- 239000002893 slag Substances 0.000 title claims abstract description 49
- 238000000034 method Methods 0.000 title claims abstract description 34
- 238000005422 blasting Methods 0.000 claims abstract description 72
- 239000000463 material Substances 0.000 claims abstract description 61
- 238000000855 fermentation Methods 0.000 claims abstract description 26
- 230000004151 fermentation Effects 0.000 claims abstract description 25
- 239000012736 aqueous medium Substances 0.000 claims abstract description 12
- 239000002245 particle Substances 0.000 claims abstract description 12
- 239000007787 solid Substances 0.000 claims description 26
- 239000002609 medium Substances 0.000 claims description 21
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- 239000000203 mixture Substances 0.000 claims description 16
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- 235000019345 sodium thiosulphate Nutrition 0.000 description 2
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- Fodder In General (AREA)
Abstract
The invention belongs to traditional Chinese medicine waste biology to extract and fermentation arts, be specifically related to the preprocess method of Rhizomatic traditional Chinese medicine slag, step: after Rhizomatic traditional Chinese medicine slag is dried, pulverize as particle diameter is not less than 20 orders, add the aqueous medium being equivalent to Rhizomatic traditional Chinese medicine slag volume 0.6-1.5 times fully to soak, and carry out steam blasting preliminary treatment, obtain steam blasting preliminary treatment material; The pretreated pressure of described steam blasting is not less than 1.0MPa, and steam blasting pretreatment time is not less than 2 minutes; The steam blasting preliminary treatment material that the preprocess method that the method obtains obtains, after enzymolysis, ferments, can be used as biological feedstuff or feed addictive; Environmental protection of the present invention, turns waste into wealth Chinese medicine slag, becomes symphysis unit biological feedstuff; Preliminary treatment material also may be used for the extraction of lignin, cellulose and hemicellulose.
Description
Technical field
The invention belongs to fermentation and the field of biological extraction of traditional Chinese medicine waste, particularly the reuse method of Chinese medicine slag.
Background technology
Chinese medicine slag is Chinese medicine remaining residue after certain solvent or other modes extract.At present these Chinese medicine slags are many by as rubbish, and this is not only the significant wastage of resource, simultaneously also can to environment.Along with the development of animal husbandry and aquaculture, traditional feed resource (all kinds of grain, wheat bran, dregs of beans etc.) can not meet the swift and violent feed market increased, and there is the drawback of striving grain with people.Except containing except the nutritional labelings such as protein, cellulose, reducing sugar, amino acid in Chinese medicine slag, also containing multiple efficacies composition.Therefore, be that fermenting raw materials produces functional feed with Chinese medicine slag, both environmental pollution can be alleviated, day by day urgent feed shortage problem can be solved again, this novel fodder has immunity, the adjustment metabolism of enhancing animal simultaneously, growth promoting effects, improving the health-care effecies such as meat, is a new technology with development potentiality.Multiple bacteria compound fermentation can produce multiply anchor-pile (C1 enzyme and Cx enzyme, cellobiase, amylase and lactase etc. as cellulase system), except having good degradation capability to cellulose substances, the functional secondary metabolites such as compound sugar can also be produced.
Summary of the invention
An object of the present invention is the preprocess method and the intermediate products that provide a kind of Rhizomatic traditional Chinese medicine slag, by the preliminary treatment material of the method process gained, can make raw material and extract lignin, cellulose or hemicellulose further.
For achieving the above object, technical scheme of the present invention is:
The preprocess method of Rhizomatic traditional Chinese medicine slag, specifically comprises the following steps: after being dried by Rhizomatic traditional Chinese medicine slag, pulverize as particle diameter is not less than 20 orders, add aqueous medium and fully soak, and carry out steam blasting preliminary treatment, obtain steam blasting preliminary treatment material; The pretreated pressure of described steam blasting is not less than 1.0MPa, and steam blasting pretreatment time is not less than 2 minutes.During immersion, add and be equivalent to Rhizomatic traditional Chinese medicine slag volume 0.6-1.5 aqueous medium doubly, soak time is no less than 8 hours.It is large that immersion process aligns demolition effect impact.0.6-1.5 aqueous medium doubly can promote hemicellulose stripping in neat explosion, improves the rate of recovery of hemicellulose.
Above-mentioned Rhizomatic traditional Chinese medicine comprises the Radix Astragali, Radix Glycyrrhizae, lily, RADIX ACONITILATERALIS PREPARATA, the bighead atractylodes rhizome, the root of herbaceous peony, Radix Isatidis, radix bupleuri, Ligusticum wallichii, ginseng, pseudo-ginseng, , the root of kudzu vine, glutinous rehmannia, rhizoma arisaematis, the wind-weed, Radix Angelicae Sinensis, the coptis, the root of large-flowered skullcap, Bulbus Fritillariae Cirrhosae, the root of Dahurain angelica, the root of bidentate achyranthes, rhizoma Gastrodiae, asparagus fern, rheum officinale, the banksia rose, the tuber of pinellia, balloonflower root, corydalis tuber, rhizoma atractylodis, sealwort, the fleece-flower root, smilax, Radix zanthoxyli, the red sage root, Radix Codonopsis, the bulb of fritillary, other Rhizomatic traditional Chinese medicine mentioned in the root of Dahurain angelica and the tuber of dwarf lilyturf and " Chinese medicine voluminous dictionary ".
Preferred by experiment, the pretreated condition of Rhizomatic traditional Chinese medicine is preferably: the pretreated pressure of steam blasting is 1.5MPa, and steam blasting pretreatment time is 5 minutes.
As preferably, be the citric acid solution of 2-5% containing mass fraction in described aqueous medium.Adopt aqueous medium as soaking solution, except economical, easy, aqueous medium is cellulosic sweller.But as preferred, the aqueous medium containing diluted acid, can impel the degraded of hemicellulose in steam blasting process and the fracture of lignin ester bond.
The steam blasting preliminary treatment material of the preprocess method acquisition of described Rhizomatic traditional Chinese medicine slag, it contains lignin, cellulose and hemicellulose.The yield that this steam blasting preliminary treatment material reclaims lignin, cellulose and hemicellulose is higher than traditional handicraft.As follow-up in this intermediate product as animal feed, for promoting mouthfeel, need remove lignin further, the removal of lignin can use prior art.
The product that two of object of the present invention is to provide a kind of reuse method to waste residue of Chinese herbs and the method to prepare and application thereof, its environmental protection, technical maturity, cost is low, the product using the method to prepare can make animal feedstuff, and this feedstuff is rich in probio.
For achieving the above object, technical scheme of the present invention is:
Based on the reuse method of described steam blasting preliminary treatment material, by described steam blasting preliminary treatment material cellulase and hemicellulase enzymolysis processing, obtain enzymolysis product, described cellulase is any one or more in endoglucanase, 1,4-BETA-D-glucancellobio-hydrolase and cellobiase and beta-glucosidase, described hemicellulase is any one or two kinds of in zytase and mannase, and the quality of described cellulase and hemicellulase is equivalent to the 1-4 ‰ of described steam blasting preliminary treatment material.
Further, described enzymolysis product is made solid medium, add probio and carry out solid fermentation, obtain fermented feed.When the raw material of steam blasting preliminary treatment material as fermented feed, before enzymolysis by conventional method except delignification, the paper " lignin separation progress " that minimizing technology is delivered see people such as Wang Hua, Chen Mingqiang, or other conventional method.
Further, described zymotechnique is specially: probio is the mixture of bacillus subtilis, lactic acid bacillus, lactobacillus acidophilus and candida utili bacterium, described bacillus subtilis: lactic acid bacillus: lactobacillus acidophilus: the weight ratio of candida utili bacterium is 2: 1: 2: 1, it is 2-6% that probio bacterial classification always inoculates weight, the aqueous medium being equivalent to solid culture and quality 50% is added with in solid medium, fermentation temperature is 35 DEG C, original ph is 6-7, fermentation time is 6 days, and when fermentation stops, thalline content is 6.5 × 10
8cfu/g.
As preferably, add the auxiliary material being equivalent to solid medium quality 10-30% in described solid medium, described auxiliary material (additive) is composed of the following components by weight: wheat bran 5-10 part, dregs of beans 5-10 part, sodium chloride 0.2-0.5 part.
The application of product in biological feedstuff prepared by described reuse method.
Three of object of the present invention is to provide a kind of biological feedstuff, and this biological feedstuff utilizes Rhizomatic traditional Chinese medicine slag for raw material, is prepared from by bio-fermentation process, and this feed nutrition enriches, and mouthfeel is good.
For achieving the above object, technical scheme of the present invention is:
Biological feedstuff, specifically composed of the following components by weight: described fermented feed 80-100 part, menthol 0.8-1.2 part, ethyl maltol 2-5 part.Wherein, adding the menthol of formula ratio and ethyl maltol, is to be in harmonious proportion the mouthfeel of Chinese medicine slag.
More beneficial effect, refers to detailed description of the invention.
Detailed description of the invention
In embodiment, the detection mode of content of cellulose is Fan Shi content of cellulose detection method.
Hemicellulose level:
1 principle: with boiling quality volume fraction be that 80% calcium nitrate solution makes starch dissolution, other water-soluble carbohydrate of interference measurement hemicellulose is removed simultaneously.After precipitation distilled water flushing, with the hydrochloric acid of higher concentration, greatly shorten the hydrolysis time of hemicellulose, be hydrolyzed the sugar juice obtained, be diluted to certain volume, with sodium hydroxide solution neutralization, the copper iodine method of total reducing sugar amount wherein measures.
Solution needed for the mensuration of 2 hemicellulose levels
(1) quality volume fraction is 80% calcium nitrate solution (2) 2mol/l hydrochloric acid (3) phenolphthalein indicator (4) 2mol/l sodium hydroxide solution (5) alkaline copper reagent (collocation method: take anhydrous Na
2cO
340g, is dissolved in 100ml distilled water, can be hot a little if molten rear tartarize 7.5g is not soluble, moves in the volumetric flask of 1000ml after cooling.Separately get in the molten 200ml distilled water of pure crystallization CuSO44.5g, again by the above-mentioned volumetric flask of this solution impouring after molten, adding distil water, to 1000ml scale, is placed for subsequent use) (6) oxalic acid-sulfuric acid mixture liquid (7) quality volume fraction be 0.5% starch solution (8) 0.1mol/l hypo solution.
3 experimental procedures (3 parallel)
(1) take the powdered biomass 0.1-0.2g of natural air drying, numerical value is n;
(2) load in 10ml centrifuge tube, add the calcium nitrate solution of 10mL80%, building and be heated to boiling, heating 5min when slowly seething with excitement;
(3) substep is centrifugal, precipitates 3 times respectively, add 5ml acetone afterwards and wash 3 times again with 10mL hot wash;
(4) in precipitation, add the hydrochloric acid of 10mL2mol/l, stir evenly, micro-45min that boils under not stopping stirring state in boiling water bath;
(5) centrifugal, residue uses 10mL distilled water flushing three times respectively, and the aqueous solution after flushing is incorporated in centrifugate.(noting: centrifugate must not have been outwelled, and be poured in triangular flask);
(6) add 1 phenolphthalein, neutralize aobvious orange red (or becoming rose-colored) with 2mol/l sodium hydroxide solution;
(7) proceed to the volumetric flask of 100mL, be diluted to scale;
(8) (note: abandon a small amount of filtrate leached at first) in dry beaker is filled into dry filter paper
(9) pipette, extract 10mL filtrate loads in Boiling tube, adds 10mL alkaline copper reagent, builds and boil 15min in boiling water;
(10) cool, add 5mL oxalic acid-sulfuric acid mixture liquid (must add when constantly stirring when acid adding mixing), then add 0.5mL0.5% starch, disappear to blue by 0.1mol/l sodium thiosulfate solution titrated, spend bmL;
(11) get 10mL alkaline copper reagent, add 5mL oxalic acid-sulfuric acid mixture liquid, then add 10mL filtrate, add the starch of 0.5mL0.5%, 0.01N sodium thiosulfate solution titrated disappears to blue, spends amL (doing blank)
(12) the cubage formula of hemicellulose in living beings:
Cellulosic percentage composition is=0.9 × 100 × [248-(a-b)] (a-b)/(1000 × n)
Embodiment 1
Rhizomatic traditional Chinese medicine in the present embodiment, for Radix Astragali slag.Radix Astragali slag take distilled water as Chinese medicine slag after solvent has fully extracted active drug composition.
After Radix Astragali slag is dried, pulverize as particle diameter 20 object powder, add the distilled water being equivalent to Chinese medicine slag volume 0.6 times and fully soak, and carry out steam blasting preliminary treatment, obtain steam blasting preliminary treatment material; The pretreated pressure of described steam blasting is 1.0MPa, and steam blasting pretreatment time is 2 minutes.
Comparative example: after Radix Astragali slag is dried, pulverize as particle diameter 20 object powder, add the distilled water being equivalent to Chinese medicine slag volume 0.6 times and fully soak, obtain preliminary treatment material; The pretreated pressure of described steam blasting is 1.0MPa, and steam blasting pretreatment time is 2 minutes.
The content of the cellulose in steam blasting preliminary treatment material and hemicellulose is detected as stated above, the content of cellulose extracted in steam blasting preliminary treatment material in the preliminary treatment material that cellulosic content mentions than comparative example improves 18%, and the hemicellulose level in the preliminary treatment material that the content extracting hemicellulose in steam blasting preliminary treatment material is mentioned than comparative example improves 13%.
Embodiment 2
Rhizomatic traditional Chinese medicine in the present embodiment, for glycyrrhiza residue.Glycyrrhiza residue take distilled water as Chinese medicine slag after solvent has fully extracted active drug composition.
After glycyrrhiza residue is dried, pulverize as particle diameter 20 object powder, add the distilled water being equivalent to Chinese medicine slag volume 1.5 times and fully soak, and carry out steam blasting preliminary treatment, obtain steam blasting preliminary treatment material; The pretreated pressure of described steam blasting is 1.0MPa, and steam blasting pretreatment time is 2 minutes.
Comparative example: after glycyrrhiza residue is dried, pulverize as particle diameter 20 object powder, add the distilled water being equivalent to Chinese medicine slag volume 1.5 times and fully soak, obtain preliminary treatment material.
The content of the cellulose in steam blasting preliminary treatment material and hemicellulose is detected as stated above, the content of cellulose extracted in steam blasting preliminary treatment material in the preliminary treatment material that cellulosic content mentions than comparative example improves 22%, and the hemicellulose level in the preliminary treatment material that the content extracting hemicellulose in steam blasting preliminary treatment material is mentioned than comparative example improves 19%.
Embodiment 3
Rhizomatic traditional Chinese medicine in the present embodiment, for glycyrrhiza residue.Glycyrrhiza residue take distilled water as Chinese medicine slag after solvent has fully extracted active drug composition.
After glycyrrhiza residue is dried, pulverize as particle diameter 30 object powder, add the distilled water being equivalent to Chinese medicine slag volume 0.8 times and fully soak (8 hours), and carry out steam blasting preliminary treatment, obtain steam blasting preliminary treatment material; The pretreated pressure of described steam blasting is 1.5MPa, and steam blasting pretreatment time is 5 minutes.
Comparative example: after glycyrrhiza residue is dried, pulverize as particle diameter 30 object powder, add the distilled water being equivalent to Chinese medicine slag volume 0.8 times and fully soak (8 hours), obtain preliminary treatment material.
The content of the cellulose in steam blasting preliminary treatment material and hemicellulose is detected as stated above, the content of cellulose extracted in steam blasting preliminary treatment material in the preliminary treatment material that cellulosic content mentions than comparative example improves 16%, and the hemicellulose level in the preliminary treatment material that the content extracting hemicellulose in steam blasting preliminary treatment material is mentioned than comparative example improves 15%.
Embodiment 4
Rhizomatic traditional Chinese medicine in the present embodiment, for glycyrrhiza residue.Glycyrrhiza residue take distilled water as Chinese medicine slag after solvent has fully extracted active drug composition.
After glycyrrhiza residue is dried, pulverize as particle diameter 30 object powder, add be equivalent to Chinese medicine slag volume 0.8 times of quality volume fraction be 2% aqueous citric acid solution fully soak (12 hours), and carry out steam blasting preliminary treatment, obtain steam blasting preliminary treatment material; The pretreated pressure of described steam blasting is 1.5MPa, and steam blasting pretreatment time is 5 minutes.
Comparative example: after glycyrrhiza residue is dried, pulverize as particle diameter 30 object powder, add and be equivalent to Chinese medicine slag volume 0.8 times of distilled water and fully soak (12 hours), obtain preliminary treatment material.
The content of the cellulose in steam blasting preliminary treatment material and hemicellulose is detected as stated above, the content of cellulose extracted in steam blasting preliminary treatment material in the preliminary treatment material that cellulosic content mentions than comparative example improves 32%, and the hemicellulose level in the preliminary treatment material that the content extracting hemicellulose in steam blasting preliminary treatment material is mentioned than comparative example improves 28%.
Embodiment 5
Respectively to extract the Radix Astragali after active drug composition, Radix Glycyrrhizae, lily, RADIX ACONITILATERALIS PREPARATA, the bighead atractylodes rhizome, the root of herbaceous peony, Radix Isatidis, radix bupleuri, Ligusticum wallichii, ginseng, pseudo-ginseng, , the root of kudzu vine, glutinous rehmannia, rhizoma arisaematis, the wind-weed, Radix Angelicae Sinensis, the coptis, the root of large-flowered skullcap, Bulbus Fritillariae Cirrhosae, the root of Dahurain angelica, the root of bidentate achyranthes, rhizoma Gastrodiae, asparagus fern, rheum officinale, the banksia rose, the tuber of pinellia, balloonflower root, corydalis tuber, rhizoma atractylodis, sealwort, the fleece-flower root, smilax, Radix zanthoxyli, the red sage root, Radix Codonopsis, the bulb of fritillary, the Chinese medicine slag of the root of Dahurain angelica and the tuber of dwarf lilyturf is raw material, pulverize as particle diameter 30 object powder, add be equivalent to Chinese medicine slag volume 0.8 times of quality volume fraction be 5% aqueous citric acid solution fully soak (12 hours), and carry out steam blasting preliminary treatment, obtain steam blasting preliminary treatment material, the pretreated pressure of described steam blasting is 1.5MPa, and steam blasting pretreatment time is 5 minutes.Obtain different steam blasting preliminary treatment material respectively.
Embodiment 6 effect observation
By to the extraction of the cellulose in embodiment 5, hemicellulose and assay, steam blasting preliminary treatment material prepared by above-mentioned various Chinese medicine slag is compared with the Chinese medicine slag without Steam explosion treatment, cellulosic yield on average improves 15%, and the yield of hemicellulose on average improves 20%.
Compared by SEM figure, without the Chinese medicine slag raw material surface smoothing of Steam explosion treatment, dense regular, the extraction of this kind of regular structure subsequent fiber element and hemicellulose; There is a large amount of holes in steam blasting preliminary treatment material surface, strip structure disappears, and material presents random structure completely, illustrates that its ionic liquid process has destroyed its crystalline texture.After Steam explosion treatment, the random structure of material makes its specific area greatly increase.
Embodiment 7
In (Radix Astragali slag) steam blasting preliminary treatment material cellulase degradation process prepared by embodiment 1 method, obtain enzymolysis product, described cellulase is the mixture of endoglucanase and 1,4-BETA-D-glucancellobio-hydrolase, the enzyme activity 300IU/100mL of this mixture, technological parameter is: under 45 DEG C of conditions, 30 revs/min, and enzymolysis time is 48 hours, the quality of described cellulase is equivalent to the one thousandth of described steam blasting preliminary treatment material, obtains solid medium after enzymolysis.
Embodiment 8
In (glycyrrhiza residue) steam blasting preliminary treatment material cellulase degradation process prepared by embodiment 4 method, obtain enzymolysis product, described cellulase is endoglucanase, the mixture of 1,4-BETA-D-glucancellobio-hydrolase and cellobiase and beta-glucosidase, its enzyme activity 300IU/100mL, described hemicellulase is the mixture of zytase and mannase, its enzyme activity 400IU/100mL, the quality of described cellulase is equivalent to 4/1000ths of described steam blasting preliminary treatment material, the quality of described hemicellulase is equivalent to the one thousandth of described steam blasting preliminary treatment material, enzymolysis process parameter is: under 45 DEG C of conditions, 30 revs/min, and enzymolysis time is that 48 hours described enzymolysis products obtain solid medium.
Embodiment 9
In order to improve mouthfeel, the neat explosion pretreatment fluid of embodiment 8 and 9, before enzymolysis, after being removed by lignin, then carries out enzymolysis, the removal technique of lignin is specially: the paper " lignin separation progress " that minimizing technology is delivered see people such as Wang Hua, Chen Mingqiang, or other conventional method.
Embodiment 10
The two kinds of solid mediums (being called for short Radix Glycyrrhizae slag charge solid medium and Radix Astragali slag charge solid medium) prepared with embodiment 9 are respectively fermentation nutriment, ferment, zymotechnique is: probio is bacillus subtilis, lactic acid bacillus, lactobacillus acidophilus, the Mixed Microbes of candida utili bacterium, mixed bacteria is bacillus subtilis by weight calculating probio, lactic acid bacillus, lactobacillus acidophilus, the ratio 2: 1: 2: 1 of candida utili bacterium, the mass ratio of moisture and solid medium is 1: 2, fermentation temperature is 35 DEG C, original ph is 6-7, fermentation time is 6 days, when fermentation stops, thalline content is 6.5 × 10
8cfu/g.After fermentation, obtain two kinds of fermented feeds.
Embodiment 11
The Radix Glycyrrhizae slag charge solid medium prepared with embodiment 9 is fermentation nutriment, ferments.Add the additive being equivalent to solid medium quality 10% in solid medium, described additive is composed of the following components by weight: 10 parts, wheat bran, dregs of beans 5 parts, 1 part, sodium chloride.Zymotechnique is: probio is bacillus subtilis: lactic acid bacillus: lactobacillus acidophilus: candida utili bacterium is 2: 1: 2: 1, the mass ratio of moisture and solid medium is 1: 2, and fermentation temperature is 35 DEG C, and original ph is 6-7, fermentation time is 6 days, and thalline content is 1.5 × 10
8cfu/g.After fermentation, fermented feed.
Embodiment 12
The Radix Astragali slag charge solid medium prepared with embodiment 9 is fermentation nutriment, ferments.Add the additive being equivalent to solid medium quality 30% in solid medium, described additive is composed of the following components by weight: 5 parts, wheat bran, dregs of beans 10 parts, 0.5 part, sodium chloride.Zymotechnique is: probio is the Mixed Microbes of bacillus subtilis, lactic acid bacillus, lactobacillus acidophilus, candida utili bacterium, mixed bacteria by weight calculate probio be bacillus subtilis, lactic acid bacillus, lactobacillus acidophilus, candida utili bacterium ratio be 2: 1: 2: 1, the mass ratio of moisture and solid medium is 1: 2, fermentation temperature is 35 DEG C, original ph is 6-7, fermentation time is 6 days, and thalline content is 1.5 × 10
8cfu/g.After fermentation, obtain fermented feed.
Embodiment 12
Feed based on the described roughage of preparation in embodiment 11, preparation biological feedstuff, specifically composed of the following components by weight: described roughage 100 parts, menthol 0.8 part, ethyl maltol 2 parts.
Embodiment 13
Feed based on the described fermented feed of preparation in embodiment 12, preparation biological feedstuff, specifically composed of the following components by weight: 80 parts, described fermented feed, menthol 1.2 parts, ethyl maltol 5 parts.Biological feedstuff prepared by embodiment 13, after testing, the indices parameters such as probio are identical with described fermented feed.Carry out sensory test with 100 empty stomach pigs, the pig of 90% selects edible described biological feedstuff.
Feed prepared by embodiment 10-13 method, safe in whole production process, smooth and easy.Semi-finished product and each Interventions Requested of finished product all reach on the indices of " microbial forage additive (enterprise) QUALITY STANDARD "; sufficient proof this Additive Production process stabilizing; the additive steady quality formulated according to " microbial forage additive production specification " (draft), safe and effective, can large-scale production be carried out.
What finally illustrate is, above embodiment is only in order to illustrate technical scheme of the present invention and unrestricted, although with reference to preferred embodiment to invention has been detailed description, those of ordinary skill in the art is to be understood that, can modify to technical scheme of the present invention or equivalent replacement, and not departing from aim and the scope of technical solution of the present invention, it all should be encompassed in the middle of right of the present invention.
Claims (8)
1. the preprocess method of Rhizomatic traditional Chinese medicine slag, it is characterized in that, specifically comprise the following steps: after Rhizomatic traditional Chinese medicine slag is dried, pulverize as particle diameter is not less than 20 orders, add the aqueous medium being equivalent to Rhizomatic traditional Chinese medicine slag volume 0.6-1.5 times fully to soak, and carry out steam blasting preliminary treatment, obtain steam blasting preliminary treatment material; Containing quality volume fraction in described aqueous medium is the citric acid of 2-5%; The pretreated pressure of described steam blasting is not less than 1.0MPa, and steam blasting pretreatment time is not less than 2 minutes.
2. the preprocess method of Rhizomatic traditional Chinese medicine slag according to claim 1, is characterized in that: the pretreated pressure of described steam blasting is 1.5MPa, and steam blasting pretreatment time is 5 minutes.
3. the steam blasting preliminary treatment material of the preprocess method acquisition of Rhizomatic traditional Chinese medicine slag according to claim 1.
4. based on the reuse method of steam blasting preliminary treatment material according to claim 3, it is characterized in that, by described steam blasting preliminary treatment material cellulase and hemicellulase enzymolysis processing, obtain enzymolysis product, described cellulase is any one or more in endoglucanase, 1,4-BETA-D-glucancellobio-hydrolase and cellobiase and beta-glucosidase, described hemicellulase is any one or two kinds of in zytase and mannase, and the quality of described cellulase and/or hemicellulase is equivalent to the 1-4 ‰ of described steam blasting preliminary treatment material;
Described enzymolysis product is made solid medium, adds probio and carry out solid fermentation, obtain fermented feed.
5. the reuse method of steam blasting preliminary treatment material according to claim 4, it is characterized in that, described zymotechnique is specially: probio is bacillus subtilis, lactic acid bacillus, the mixture of lactobacillus acidophilus and candida utili bacterium, described bacillus subtilis: lactic acid bacillus: lactobacillus acidophilus: the weight ratio of candida utili bacterium is 2: 1: 2: 1, it is 2-6% that probio bacterial classification always inoculates weight, the aqueous medium being equivalent to solid culture and quality 50% is added with in solid medium, fermentation temperature is 35 DEG C, original ph is 6-7, fermentation time is 6 days, when fermentation stops, thalline content is 6.5 × 10
8cfu/g.
6. the reuse method of steam blasting preliminary treatment material according to claim 5, it is characterized in that, the auxiliary material being equivalent to solid medium quality 10-30% is added in described solid medium, described auxiliary material is composed of the following components by weight: wheat bran 5-10 part, dregs of beans 5-10 part, sodium chloride 0.2-0.5 part.
7. the application of product in biological feedstuff prepared of reuse method according to claim 4.
8. a biological fermentation feed, is characterized in that, specifically composed of the following components by weight: fermented feed 80-100 part that the reuse method of claim 4 is obtained, menthol 0.8-1.2 part, ethyl maltol 2-5 part.
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