CN101636505B - 在酶反应中在样品中合成cDNA的方法 - Google Patents

在酶反应中在样品中合成cDNA的方法 Download PDF

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CN101636505B
CN101636505B CN2007800303214A CN200780030321A CN101636505B CN 101636505 B CN101636505 B CN 101636505B CN 2007800303214 A CN2007800303214 A CN 2007800303214A CN 200780030321 A CN200780030321 A CN 200780030321A CN 101636505 B CN101636505 B CN 101636505B
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enzyme
reaction
oligonucleotide
reverse transcriptase
rna
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CN101636505A (zh
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H·恩格尔
S·耶拉米利
M·科鲁兹
D·洛弗特
C·科菲哈格
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Qiagen GmbH
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Qiagen GmbH
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN2007800303214A 2006-08-14 2007-08-13 在酶反应中在样品中合成cDNA的方法 Active CN101636505B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE102006038113.0 2006-08-14
DE102006038113A DE102006038113A1 (de) 2006-08-14 2006-08-14 Verfahren zur Synthese einer cDNA in einer Probe in einer enzymatischen Reaktion und gleichzeitigen Bereitstellung eines ersten Enzyms mit Polyadenylierungsaktivität und eines zweiten Enzyms mit reverser Transkriptaseaktivität
PCT/EP2007/058369 WO2008020008A1 (de) 2006-08-14 2007-08-13 VERFAHREN ZUR SYNTHESE EINER cDNA IN EINER PROBE IN EINER ENZYMATISCHEN REAKTION

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CN101636505A CN101636505A (zh) 2010-01-27
CN101636505B true CN101636505B (zh) 2012-09-05

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CN2007800303214A Active CN101636505B (zh) 2006-08-14 2007-08-13 在酶反应中在样品中合成cDNA的方法
CN2008801034578A Active CN101802222B (zh) 2006-08-14 2008-02-13 利用酶促反应合成样品中的cDNA的方法

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US (3) US9217173B2 (https=)
EP (1) EP2052086B1 (https=)
JP (1) JP5409360B2 (https=)
CN (2) CN101636505B (https=)
AU (1) AU2007286300B2 (https=)
DE (1) DE102006038113A1 (https=)
WO (1) WO2008020008A1 (https=)

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DE102006038113A1 (de) * 2006-08-14 2008-02-21 Qiagen Gmbh Verfahren zur Synthese einer cDNA in einer Probe in einer enzymatischen Reaktion und gleichzeitigen Bereitstellung eines ersten Enzyms mit Polyadenylierungsaktivität und eines zweiten Enzyms mit reverser Transkriptaseaktivität
AU2008286254B2 (en) * 2007-08-13 2014-01-30 Qiagen Gmbh Method for synthesizing a cDNA in a sample in an enzymatic reaction
WO2010006814A1 (en) 2008-07-18 2010-01-21 Qiagen Gmbh Method for determining the origin of a sample
AU2010207826B2 (en) 2009-02-02 2013-10-03 Qiagen Gmbh Method for quantification of small RNA species
EP2280080A1 (en) * 2009-07-31 2011-02-02 Qiagen GmbH Method of normalized quantification of nucleic acids using anchor oligonucleotides and adapter oligonucleotides
EP2280079A1 (en) * 2009-07-31 2011-02-02 Qiagen GmbH Ligation-based method of normalized quantification of nucleic acids
EP2280081A1 (en) * 2009-07-31 2011-02-02 Qiagen GmbH Method of normalized quantification of RNA
KR101818126B1 (ko) * 2011-02-09 2018-01-15 (주)바이오니아 열안정성이 증가된 역전사효소
WO2013111800A1 (ja) 2012-01-25 2013-08-01 独立行政法人科学技術振興機構 Ｈｉｖ検出用オリゴヌクレオチド、ｈｉｖ検出キット、及びｈｉｖ検出方法
EP2912197B1 (en) 2012-10-24 2019-08-07 Takara Bio USA, Inc. Template switch-based methods for producing a product nucleic acid
WO2014071322A1 (en) 2012-11-02 2014-05-08 Life Technologies Corporation Small RNA Capture, Detection and Quantification
EP4389888A3 (en) 2013-10-17 2024-09-04 Takara Bio USA, Inc. Methods for adding adapters to nucleic acids and compositions for practicing the same
WO2015094861A1 (en) 2013-12-17 2015-06-25 Clontech Laboratories, Inc. Methods for adding adapters to nucleic acids and compositions for practicing the same
WO2016092062A1 (en) * 2014-12-11 2016-06-16 Qiagen Gmbh Method for improving the efficiency of cdna generation
US10563250B2 (en) 2015-03-13 2020-02-18 Life Technologies Corporation Methods, compositions and kits for small RNA capture, detection and quantification
CN107541507A (zh) * 2016-06-29 2018-01-05 厦门大学 一种rna反转录扩增方法
US11993800B2 (en) * 2017-05-26 2024-05-28 Nuclera Ltd. Use of terminal transferase enzyme in nucleic acid synthesis
WO2020127620A1 (en) * 2018-12-20 2020-06-25 F. Hoffmann-La Roche Ag Detection of target nucleic acid by solid-phase molography
WO2020227736A2 (en) * 2019-05-06 2020-11-12 The Procter & Gamble Company Detection of microbial endotoxins in oral samples using aptamers
CA3178255A1 (en) * 2020-05-15 2021-11-18 Krishna KANNAN On demand synthesis of polynucleotide sequences
US20220056510A1 (en) * 2020-08-20 2022-02-24 Detect, Inc. Enzymatic tablet and uses thereof
EP4301499A4 (en) 2021-03-05 2025-01-22 Enumerix, Inc. SYSTEMS AND METHODS FOR GENERATING DROPLETS AND PERFORMING DIGITAL ANALYSES
WO2023025259A1 (zh) * 2021-08-25 2023-03-02 卓越精准医疗有限公司 检测微小rna的方法和试剂盒

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1763223A (zh) * 2004-10-19 2006-04-26 中国人民解放军军事医学科学院放射医学研究所 miRNA检测方法及试剂盒

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Publication number Priority date Publication date Assignee Title
WO1999043650A2 (en) 1998-02-25 1999-09-02 Smithkline Beecham Plc Process(es) for the preparation of 6-trifluoromethyl-indoline derivatives
DE69900592T2 (de) * 1998-02-27 2002-08-22 Pamgene B.V., Den Bosch Verfahren zur unspezifischen amplifizierung einer nukleinsäure
US6300069B1 (en) * 1999-05-03 2001-10-09 Qiagen Gmbh Generation and amplification of nucleic acids from ribonucleic acids
US7141372B2 (en) * 2002-01-18 2006-11-28 Health Research Incorporated Universal RT-coupled PCR method for the specific amplification of mRNA
CA2501983A1 (en) * 2002-10-30 2004-05-27 Pamgene B.V. Improved methods for generating multiple rna copies
CA2551267C (en) * 2003-12-23 2012-05-01 Genomic Health, Inc. Universal amplification of fragmented rna
DE102006038113A1 (de) * 2006-08-14 2008-02-21 Qiagen Gmbh Verfahren zur Synthese einer cDNA in einer Probe in einer enzymatischen Reaktion und gleichzeitigen Bereitstellung eines ersten Enzyms mit Polyadenylierungsaktivität und eines zweiten Enzyms mit reverser Transkriptaseaktivität
US8314220B2 (en) * 2007-01-26 2012-11-20 Agilent Technologies, Inc. Methods compositions, and kits for detection of microRNA

Patent Citations (1)

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Publication number Priority date Publication date Assignee Title
CN1763223A (zh) * 2004-10-19 2006-04-26 中国人民解放军军事医学科学院放射医学研究所 miRNA检测方法及试剂盒

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Publication number Publication date
AU2007286300A1 (en) 2008-02-21
AU2007286300B2 (en) 2012-11-22
CN101802222A (zh) 2010-08-11
US20120202198A1 (en) 2012-08-09
CN101802222B (zh) 2013-07-03
CN101636505A (zh) 2010-01-27
EP2052086A1 (de) 2009-04-29
JP2010500044A (ja) 2010-01-07
JP5409360B2 (ja) 2014-02-05
US9611506B2 (en) 2017-04-04
WO2008020008A1 (de) 2008-02-21
US9217173B2 (en) 2015-12-22
DE102006038113A1 (de) 2008-02-21
EP2052086B1 (de) 2013-03-13
US20110124050A1 (en) 2011-05-26
US20160130634A1 (en) 2016-05-12

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