CN101631876A - 2型糖尿病的遗传易感性变体 - Google Patents
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Abstract
关联性分析显示出某些遗传变体是2型糖尿病的易感性变体。本发明涉及这些易感性变体的诊断应用,包括在个体中确定对2型糖尿病的增加的易感性的方法,以及确定对2型糖尿病降低的易感性的方法。本发明还涉及了在本文描述的变体的基础上用于测定对2型糖尿病的易感性的试剂盒。
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发明背景
糖尿病是一种代谢疾病,其中碳水化合物的利用被降低,脂类和蛋白质的利用被增加,由胰岛素的绝对或相对缺乏所引起。在更严重的病例中,糖尿病的特征为慢性高血糖、糖尿、水和电解质损失、酮酸中毒和昏迷。长期并发症包括发展出神经病、视网膜病、肾病、大小血管中的普遍退行性变化以及对感染的增加的易感性。最常见的糖尿病形式是II型、非胰岛素依赖性糖尿病,其特征为由于胰岛素分泌受损和靶组织中的胰岛素抗性所引起的高血糖。遗传和环境因素都对这种疾病有贡献。例如,肥胖在这种疾病的发生中扮演了重要角色。2型糖尿病通常是一种逐渐发作的温和形式的糖尿病。
2型糖尿病与健康的关联是巨大的。1995年,在世界范围内有1.35亿成年人患有糖尿病。据估计,到2025年,将有接近3亿人患上糖尿病(King,H.等,Diabetes Care,21(9):1414-1431(1998))。在冰岛,在成年人群体中2型糖尿病的发病率为2.5%(Vilbergsson,S.等,DiabetMed.,14(6):491-498(1997)),其中包含了大约5000个34岁以上患有疾病的人。
2型糖尿病的特征为高血糖,这可以通过例如胰岛素分泌受损、外周组织中的胰岛素抗性、以及肝脏的增加的葡萄糖输出等机制来发生。大多数2型糖尿病患者经受了慢性高血糖的严重并发症,包括肾病、神经病、视网膜病和心血管疾病的加速发展。在世界范围内2型糖尿病的发病率目前为6%,但是预计在下一个十年中将上升(Amos,A.F.,McCarty,D.J.,Zimmet,P.,Diabet Med 14 Suppl 5,Sl(1997))。这种2型糖尿病发病率的增加归因于人群年龄的增加和肥胖症的升高。
有证据表明在2型糖尿病的风险中有遗传的成分,包括在各种不同种族的组之间发病率的差异(Zimmet,P.等,Am J Epidemiol 118,673(1983);Knowler,W.C,Pettitt,D.J.,Saad,M.F.,Bennett,P.H.,DiabetesMetab Rev 6,1(1990)),同卵双胞胎中与异卵双胞胎相比更高的一致率(Newman,B.等,Diabetologia 30,763(1987);Barnett,A.H.,Eff,C,Leslie,R.D.,Pyke,D.A.,Diabetologia 20,87(1981)),以及在欧洲人群体中2型糖尿病的大约3.5的兄弟姐妹间相对风险(λs)(Gloyn,A.L.,Ageing Res Rev 2,111(2003))。
迄今为止,已经使用了两种方法来搜索与2型糖尿病相关的基因。已经测试了候选基因中的单核苷酸多态性(SNPs)的关联性,总的来说,它们没有被重复出来,或仅仅赋予了2型糖尿病的不大的风险——其中最广泛报道的是过氧化物酶体增殖物激活受体γ基因(PPARG2)中的保护性Pro12Ala多态性(Altshuler,D.等,Nat Genet 26,76(2000))和钾内向整流通道亚家族J的11号成员基因(KIR6.2)中的风险性多态性(Gloyn A.L.等,Diabetes 52,568(2003))。
在患有常见形式的2型糖尿病的家族中进行的基因组范围内的连锁扫描得到了几个位点,国际研究团体的主要焦点是在许多人群中观察到的位于1、12和20号染色体上的位点(Gloyn,A.L.,Ageing Res Rev2,111(2003))。这些位点中的基因还没有被揭示。但是,在墨西哥裔美国人中,从染色体2q上的位点分离出了钙蛋白酶(calpain)10(CAPN10)基因(Horikawa,Y.等,Nat Genet 26,163(2000))。对于罕见的孟德尔形式的2型糖尿病、即青年人中的成年发病型糖尿病(MODY)来说,通过定位克隆获得了6个基因(Gloyn,A.L.,AgeingRes Rev 2,111(2003))。
在冰岛人群中,报道了2型糖尿病与染色体5q的基因组范围内的显著连锁(Reynisdottir,I.等,Am J Hum Genet 73,323(2003));在同一项研究中,还报道了与10q和12q连锁的提示性的证据。在墨西哥裔美国人中,已经观察到了与10q区域的连锁(Duggirala,R.等,Am JHum Genet 64,1127(1999))。
类转录因子7的基因2(TCF7L2;以前称为TCF4)已经与2型糖尿病相关联(P=2.1x10(-9))(Grant,S.F.等,Nat Genet 38,320(2006))。在冰岛群体中与基因的内含子3中的微卫星标记物DG10S478的关联性(P=2.1x10(-9))的原始发现在丹麦人群体(P=4.8x10(-3))和美国人群体(P=3.3x10(-9))中得到了重复。与非携带者相比,风险等位基因的杂合和纯合携带者(分别为群体的38%和7%)的相对风险为1.45和2.41。这相当于群体可归因风险为21%。现在,TCF7L2变体的关联性已经在10项独立研究中得到了重复,在研究的不同群体中发现了相似的相对风险。TCF7L2基因产物是含有高迁移率组盒的转录因子,以前被暗示与血糖体内平衡有关。它被认为通过Wnt信号转导途径在肠内分泌细胞中调节高血糖素原基因的表达而起作用。
尽管在了解2型糖尿病的遗传学方面取得了进展,但疾病的高发病率以及不断增加的患病人群显示出,对于定义其它与2型糖尿病有关的遗传风险、以便更精确地定义相关的风险因子,在医学上仍有尚未得到满足的需求。具有受损的空腹葡萄糖水平或受损的葡萄糖耐受性的人是无症状的,但是有发生2型糖尿病的高风险。目前,在这个高风险组中,对于将生活方式干预可能是疾病预防的最佳选择的个体、与预防性医学治疗可能更适合的个体区分开来,只有非常少的信息。对易感基因的鉴定将允许对疾病的病理生理学有更好的理解,并对患者具有直接的益处,它将有助于用于诊断和治疗的更好的方法的产生。用于预防2型糖尿病的治疗药剂也是需要的。
发明简述
本发明涉及了通过对已发现与2型糖尿病的增加的或减少的易感性有关联的某些标记物或单倍型进行评估,来诊断对2型糖尿病的增加的易感性的方法,以及诊断对2型糖尿病的减少的易感性或诊断针对2型糖尿病的保护的方法。
第一种情况下,本发明涉及在人类个体中确定对2型糖尿病的易感性的方法,包括在从个体获得的核酸样品中确定至少一个多态性标记物的至少一个等位基因的存在或不存在,其中至少一个多态性标记物选自表10-12中显示的标记物、以及与其连锁不平衡的标记物,其中确定至少一个等位基因的存在或不存在是对2型糖尿病易感性的指示。在一个实施方案中,至少一个多态性标记物选自表10-12和14中显示的标记物。在可选的情况下,确定对2型糖尿病的易感性的方法,是诊断对2型糖尿病的易感性的方法。
在一个实施方案中,至少一个多态性标记物显示在SEQ ID NO:1、SEQ ID NO:2或SEQ ID NO:3中。在另一个实施方案中,至少一个多态性标记物包括选自下列的至少一个标记物:rs2497304(SEQ ID NO:16)、rs947591(SEQ ID NO:30)、rs10882091(SEQ ID NO:4)、rs7914814(SEQ ID NO:24)、rs6583830(SEQ ID NO:20)、rs2421943(SEQ IDNO:15)、rs6583826(SEQ ID NO:19)、rs7752906(SEQ ID NO:32)、rs1569699(SEQ ID NO:6)、rs7756992(SEQ ID NO:21)、rs9350271(SEQ ID NO:33)、rs9356744(SEQ ID NO:34)、rs9368222(SEQ IDNO:35)、rs10440833(SEQ ID NO:36)、rs6931514(SEQ ID NO:37)、rs1860316(SEQ ID NO:10)、rs1981647(SEQ ID NO:11)、rs1843622(SEQ ID NO:9)、rs2191113(SEQ ID NO:13)、rs9890889(SEQ IDNO:31),以及与其连锁不平衡的标记物。在另一个实施方案中,至少一个多态性标记物包括与选自表22、表23和表24中显示的标记物中的一个或多个标记物强烈连锁不平衡的至少一个标记物,强烈连锁不平衡被定义为数值|D′|大于0.8和/或r2大于0.2。在一个优选实施方案中,至少一个多态性标记物选自标记物rs7752906(SEQ ID NO:32)、rs1569699(SEQ ID NO:6)、rs7756992(SEQ ID NO:21)、rs9350271(SEQ ID NO:33)、rs9356744(SEQ ID NO:34)、rs9368222(SEQ IDNO:35)、rs10440833(SEQ ID NO:36)、rs6931514(SEQ ID NO:37),以及与其连锁不平衡的标记物。在另一个优选实施方案中,至少一个多态性标记物选自标记物rs7752906(SEQ ID NO:32)、rs1569699(SEQID NO:6)、rs7756992(SEQ ID NO:21)、rs9350271(SEQ ID NO:33)、rs9356744(SEQ ID NO:34)、rs9368222(SEQ ID NO:35)、rs10440833(SEQ ID NO:36)和rs6931514(SEQ ID NO:37)。在一个实施方案中,至少一个标记物选自标记物rs7756992(SEQ ID NO:21)以及与其连锁不平衡的标记物。在另一个实施方案中,至少一个标记物选自表22中显示的标记物。在另一个实施方案中,至少一个标记物选自标记物rs10882091(SEQ ID NO:4)以及与其连锁不平衡的标记物。在另一个实施方案中,至少一个标记物选自表23中显示的标记物。在另一个实施方案中,至少一个标记物选自标记物rs2191113(SEQ ID NO:13)以及与其连锁不平衡的标记物。在另一个实施方案中,至少一个标记物选自表24中显示的标记物。
在一个实施方案中,测定2型糖尿病的易感性、或诊断2型糖尿病的易感性的方法,还包括评估个体中至少一种单倍型的频率。在一个这样的实施方案中,至少一种单倍型选自含有表1-6中显示的至少一个多态性标记物以及与其连锁不平衡的多态性标记物的单倍型。在另一个实施方案中,至少一种单倍型选自含有至少一个多态性标记物的单倍型,该至少一个多态性标记物选自下列标记物中的至少一个标记物:rs2497304(SEQ ID NO:16)、rs947591(SEQ ID NO:30)、rs10882091(SEQ ID NO:4)、rs7914814(SEQ ID NO:24)、rs6583830(SEQ IDNO:20)、rs2421943(SEQ ID NO:15)、rs6583826(SEQ ID NO:19)、rs7752906(SEQ ID NO:32)、rs1569699(SEQ ID NO:6)、rs7756992(SEQ ID NO:21)、rs9350271(SEQ ID NO:33)、rs9356744(SEQ IDNO:34)、rs9368222(SEQ ID NO:35)、rs10440833(SEQ ID NO:36)、rs6931514(SEQ ID NO:37)、rs1860316(SEQ ID NO:10)、rs 1981647(SEQ ID NO:11)、rs1843622(SEQ ID NO:9)、rs2191113(SEQ IDNO:13)、rs9890889(SEQ ID NO:31),以及与其连锁不平衡的标记物。在另一个实施方案中,至少一种单倍型选自表1-6和14中显示的单倍型。
第二种情况下,本发明涉及在人类个体中确定对2型糖尿病的易感性的方法,包括确定至少一个多态性标记物中的至少一个风险等位基因是否存在于个体的基因型数据组中,其中至少一个多态性标记物选自表10-12中显示的标记物以及与其连锁不平衡的标记物,其中确定了至少一个风险等位基因的存在是个体中对2型糖尿病的增加的易感性的指示。在一个实施方案中,基因型数据组包含关于标记物身份以及个体的等位基因状态的信息,即关于个体携带的两个等位基因的标记物身份的信息。基因型数据组可以包含关于一个或多个标记物、包括两个或两个以上标记物、三个或三个以上标记物、五个或五个以上标记物、一百个或以上标记物等的等位基因信息。在某些实施方案中,基因型数据组包含来自个体的全基因组评估的含有成百上千个标记物、或甚至一百万个或以上标记物的基因型信息。
在一个实施方案中,至少一个多态性标记物显示在SEQ ID NO:1、SEQ ID NO:2或SEQ ID NO:3中。在另一个实施方案中,至少一个多态性标记物包括选自下列的至少一个标记物:rs2497304(SEQ ID NO:16)、rs947591(SEQ ID NO:30)、rs10882091(SEQ ID NO:4)、rs7914814(SEQ ID NO:24)、rs6583830(SEQ ID NO:20)、rs2421943(SEQ IDNO:15)、rs6583826(SEQ ID NO:19)、rs7752906(SEQ ID NO:32)、rs1569699(SEQ ID NO:6)、rs7756992(SEQ ID NO:21)、rs9350271(SEQ ID NO:33)、rs9356744(SEQ ID NO:34)、rs9368222(SEQ IDNO:35)、rs10440833(SEQ ID NO:36)、rs6931514(SEQ ID NO:37)、rs1860316(SEQ ID NO:10)、rs1981647(SEQ ID NO:11)、rs1843622(SEQ ID NO:9)、rs2191113(SEQ ID NO:13)、rs9890889(SEQ IDNO:31),以及与其连锁不平衡的标记物。在另一个实施方案中,至少一个多态性标记物包括与选自表22、表23和表24中显示的标记物中的一个或多个标记物强烈连锁不平衡的至少一个标记物,强烈连锁不平衡被定义为数值|D′|大于0.8和/或r2大于0.2。在一个优选实施方案中,至少一个多态性标记物选自标记物rs7752906(SEQ ID NO:32)、rs1569699(SEQ ID NO:6)、rs7756992(SEQ ID NO:21)、rs9350271(SEQ ID NO:33)、rs9356744(SEQ ID NO:34)、rs9368222(SEQ IDNO:35)、rs10440833(SEQ ID NO:36)、rs6931514(SEQ ID NO:37),以及与其连锁不平衡的标记物。在另一个优选实施方案中,至少一个多态性标记物选自标记物rs7752906(SEQ ID NO:32)、rs1569699(SEQID NO:6)、rs7756992(SEQ ID NO:21)、rs9350271(SEQ ID NO:33)、rs9356744(SEQ ID NO:34)、rs9368222(SEQ ID NO:35)、rs10440833(SEQ ID NO:36)和rs6931514(SEQ ID NO:37)。在一个实施方案中,至少一个标记物选自标记物rs7756992(SEQ ID NO:21)以及与其连锁不平衡的标记物。在另一个实施方案中,至少一个标记物选自表22中显示的标记物。在另一个实施方案中,至少一个标记物选自标记物rs10882091(SEQ ID NO:4)以及与其连锁不平衡的标记物。在另一个实施方案中,至少一个标记物选自表23中显示的标记物。在另一个实施方案中,至少一个标记物选自标记物rs2191113(SEQ ID NO:13)以及与其连锁不平衡的标记物。在另一个实施方案中,至少一个标记物选自表24中显示的标记物。在另一个实施方案中,至少一个标记物选自与8号染色体上118,032,398和118,258,134位(人类基因组装配图NCBI Build 36)之间的SLC30A基因连锁不平衡的标记物。在一个这样的实施方案中,至少一个标记物位于SLC30A基因中。
在一个实施方案中,测定2型糖尿病的易感性、或诊断2型糖尿病的易感性的方法,还包括评估个体中至少一种单倍型的频率。在一个这样的实施方案中,至少一种单倍型选自含有表1-6中显示的至少一个多态性标记物以及与其连锁不平衡的多态性标记物的单倍型。在另一个实施方案中,至少一种单倍型选自含有至少一个多态性标记物的单倍型,该至少一个多态性标记物选自下列标记物中的至少一个标记物:rs2497304(SEQ ID NO:16)、rs947591(SEQ ID NO:30)、rs10882091(SEQ ID NO:4)、rs7914814(SEQ ID NO:24)、rs6583830(SEQ IDNO:20)、rs2421943(SEQ ID NO:15)、rs6583826(SEQ ID NO:19)、rs7752906(SEQ ID NO:32)、rs1569699(SEQ ID NO:6)、rs7756992(SEQ ID NO:21)、rs9350271(SEQ ID NO:33)、rs9356744(SEQ IDNO:34)、rs9368222(SEQ ID NO:35)、rs10440833(SEQ ID NO:36)、rs6931514(SEQ ID NO:37)、rs1860316(SEQ ID NO:10)、rs1981647(SEQ ID NO:11)、rs1843622(SEQ ID NO:9)、rs2191113(SEQ IDNO:13)、rs9890889(SEQ ID NO:31),以及与其连锁不平衡的标记物。在另一个实施方案中,至少一种单倍型选自表1-6和14中显示的单倍型。
在本发明的某些实施方案中,在来自个体的核酸样品中至少一个多态性标记物的至少一个风险等位基因的存在的确定,表明了对2型糖尿病的增加的易感性。在一个实施方案中,增加的易感性的特征为相对风险(RR)或比值比(OR)为至少1.15。在另一个实施方案中,增加的易感性的特征为相对风险(RR)或比值比(OR)为至少1.20。
在某些实施方案中,rs2497304等位基因A、rs947591等位基因A、rs10882091等位基因C、rs7914814等位基因T、rs6583830等位基因A、rs2421943等位基因G、rs6583826等位基因G、rs7752906等位基因A、rs1569699等位基因C、rs7756992等位基因G、rs9350271等位基因A、rs9356744等位基因C、rs9368222等位基因A、rs10440833等位基因A、rs6931514等位基因G、rs1860316等位基因A、rs1981647等位基因C、rs1843622等位基因T、rs2191113等位基因A和/或rs9890889等位基因A的存在,表明了2型糖尿病的增加的易感性。
在具体的实施方案中,在来自个体的核酸样品中至少一个保护性等位基因的存在,指示了对2型糖尿病的降低的易感性。在另一个实施方案中,来自个体的核酸样品中至少一个风险等位基因的不存在,指示了对2型糖尿病的降低的易感性。
本发明的方法的具体实施方案涉及与个体中胰岛素响应和/或受损的葡萄糖耐受性进一步相关的至少一个标记物或单倍型。
在其它实施方案中,风险标记物的至少一个等位基因或单倍型的存在或确定,指示了对2型糖尿病的增加的易感性,其中至少一个等位基因或单倍型还指示了降低的胰岛素响应和/或受损的葡萄糖耐受性。
在本发明的某些实施方案中,连锁不平衡的特征为数值|D′|大于0.8和/或r2大于0.2。但是,在其它实施方案中,其它的r2和|D′|的值也是可能的,正如在本文中进一步详细描述的,这样的实施方案也在本发明要求保护的范围内。
本发明的另一方面涉及了在人类个体中评估对2型糖尿病的易感性的方法,包括在来自个体的核酸中筛选SEQ ID NO:1、SEQ ID NO:2或SEQ ID NO:3中与人类群体中2型糖尿病的增加的发病率相关的至少一个多态性标记物或单倍型,其中在核酸中至少一个多态性的风险标记物等位基因或风险单倍型的存在,使个体被鉴定为具有对糖尿病的升高的易感性,其中在核酸中至少一个风险标记物等位基因或风险单倍型的不存在,使个体被鉴定为不具有升高的易感性。
在一个实施方案中,多态性或单倍型选自rs2497304(SEQ ID NO:16)、rs947591(SEQ ID NO:30)、rs10882091(SEQ ID NO:4)、rs7914814(SEQ ID NO:24)、rs6583830(SEQ ID NO:20)、rs2421943(SEQ IDNO:15)、rs6583826(SEQ ID NO:19)、rs7752906(SEQ ID NO:32)、rs1569699(SEQ ID NO:6)、rs7756992(SEQ ID NO:21)、rs9350271(SEQ ID NO:33)、rs9356744(SEQ ID NO:34)、rs9368222(SEQ IDNO:35)、rs10440833(SEQ ID NO:36)、rs6931514(SEQ ID NO:37)、rs1860316(SEQ ID NO:10)、rs1981647(SEQ ID NO:11)、rs1843622(SEQ ID NO:9)、rs2191113(SEQ ID NO:13)、rs9890889(SEQ IDNO:31),以及与其连锁不平衡的标记物,其中连锁不平衡的特征为数值|D′|大于0.8和/或r2大于0.2。
本发明的某些实施方案还包括在核酸中筛选与LD区块C06(SEQID NO:1)、LD区块C10(SEQ ID NO:2)和LD区块C17(SEQ ID NO:3)不相关的2型糖尿病的至少一个风险遗传变体的存在的步骤。在具体的实施方案中,这种另外的遗传变体可以包括任何已经被鉴定为2型糖尿病的易感性或风险变体的变体,包括在本文中描述的其它变体。在一个实施方案中,该步骤包括筛选核酸中TCF7L2基因中的2型糖尿病的至少一个风险变体的至少一个风险等位基因的存在或不存在,其中至少一个风险等位基因的存在的确定,指示了2型糖尿病的增加的易感性。在另一个实施方案中,TCF7L2基因中的至少一个风险变体选自标记物DG10S478、rs12255372、rs7895340、rs11196205、rs7901695、rs7903146、rs12243326和rs4506565,以及与其连锁不平衡的标记物。
在本发明的另一方面,被发现与2型糖尿病相关、并可用于确定对2型糖尿病的易感性的标记物或单倍型的存在,指示了对象对2型糖尿病的具体治疗模式的不同的响应速度。
另一方面,本发明涉及了用于在人类个体中评估对2型糖尿病的易感性的标记物的鉴定方法,该方法包括:
鉴定SEQ ID NO:1、SEQ ID NO:2或SEQ ID NO:3中的至少一个多态性标记物,或与其连锁不平衡的至少一个多态性标记物;
确定被诊断患有2型糖尿病或具有2型糖尿病易感性的个体的样品的基因型状态;以及
确定对照个体的样品的基因型状态;
其中在被诊断患有2型糖尿病或具有2型糖尿病易感性的个体中至少一个多态性的至少一个等位基因的频率,与对照样品中至少一个等位基因的频率相比的显著差异,表明了至少一个多态性可用于评估对2型糖尿病的易感性。
在一个实施方案中,“显著的”是通过统计学方法确定的,例如,差异是统计学显著的。在一个这样的实施方案中,统计学显著性的特征为P-值小于0.05。在其它实施方案中,统计学显著性的特征为P-值小于0.01、小于0.001、小于0.0001、小于0.00001、小于0.000001、小于0.0000001、小于0.0000000001、或小于0.00000001。
在一个实施方案中,至少一个多态性标记物与选自下列标记物的至少一个标记物连锁不平衡,其中连锁不平衡的特征为数值r2大于0.2和/或|D′|大于0.8:rs2497304(SEQ ID NO:16)、rs947591(SEQ IDNO:30)、rs10882091(SEQ ID NO:4)、rs7914814(SEQ ID NO:24)、rs6583830(SEQ ID NO:20)、rs2421943(SEQ ID NO:15)、rs6583826(SEQ ID NO:19)、rs7752906(SEQ ID NO:32)、rs1569699(SEQ IDNO:6)、rs7756992(SEQ ID NO:21)、rs9350271(SEQ ID NO:33)、rs9356744(SEQ ID NO:34)、rs9368222(SEQ ID NO:35)、rs10440833(SEQ ID NO:36)、rs6931514(SEQ ID NO:37)、rs1860316(SEQ IDNO:10)、rs1981647(SEQ ID NO:11)、rs1843622(SEQ ID NO:9)、rs2191113(SEQ ID NO:13)、rs9890889(SEQ ID NO:31)。
在一个实施方案中,在被诊断患有2型糖尿病或具有2型糖尿病易感性的个体中至少一个多态性的至少一个等位基因的频率,与对照样品中至少一个等位基因的频率相比的增加,表明了至少一个多态性可用于评估对2型糖尿病的增加的易感性。在另一个实施方案中,在被诊断患有2型糖尿病或具有2型糖尿病易感性的个体中至少一个多态性的至少一个等位基因的频率,与对照样品中至少一个等位基因的频率相比的降低,表明了至少一个多态性可用于评估对2型糖尿病的降低的易感性,或针对2型糖尿病的保护。
另一方面,本发明涉及对从人类个体获得的核酸样品进行基因分型的方法,包括确定样品中至少一个多态性标记物的至少一个等位基因的存在或不存在,其中至少一个标记物选自rs2497304(SEQ ID NO:16)、rs947591(SEQ ID NO:30)、rs10882091(SEQ ID NO:4)、rs7914814(SEQ ID NO:24)、rs6583830(SEQ ID NO:20)、rs2421943(SEQ IDNO:15)、rs6583826(SEQ ID NO:19)、rs7752906(SEQ ID NO:32)、rs1569699(SEQ ID NO:6)、rs7756992(SEQ ID NO:21)、rs9350271(SEQ ID NO:33)、rs9356744(SEQ ID NO:34)、rs9368222(SEQ IDNO:35)、rs10440833(SEQ ID NO:36)、rs6931514(SEQ ID NO:37)、rs1860316(SEQ ID NO:10)、rs1981647(SEQ ID NO:11)、rs1843622(SEQ ID NO:9)、rs2191113(SEQ ID NO:13)、rs9890889(SEQ IDNO:31),以及与其连锁不平衡的标记物,其中至少一个多态性标记物的至少一个等位基因的存在或不存在的确定,可以预测对2型糖尿病的易感性。
在一个实施方案中,基因分型包括使用位于至少一个多态性标记物两侧的核苷酸引物对,通过聚合酶链反应(PCR)扩增含有至少一个多态性标记物的核酸的区段。在另一个实施方案中,基因分型使用选自等位基因特异性探针杂交、等位基因特异性引物延伸、等位基因特异性扩增、核酸测序、5’-外切核酸酶消化、分子信标分析、寡核苷酸连接分析、大小分析以及单链构象分析的方法来进行。在一个具体的实施方案中,方法包括等位基因特异性探针杂交。在另一个实施方案中,方法包括DNA测序。在一个优选实施方案中,方法包括:
1)将核酸的拷贝与检测寡核苷酸探针和增强子寡核苷酸探针在允许寡核苷酸探针与核酸特异性杂交的条件下相接触;
其中
a)检测寡核苷酸探针长度为5-100个核苷酸,并与含有至少一个多态性位点、其核苷酸序列在SEQ ID NO:1、SEQ ID NO:2或SEQ ID NO:3中给出的核酸的第一个片段特异性杂交;
b)检测寡核苷酸探针在其3’末端含有可检测标记,在其5’末端含有淬灭基团;
c)增强子寡核苷酸长度为5-100个核苷酸,并与相对于寡核苷酸探针5’方向的核苷酸序列的第二个区段互补,以便当两个寡核苷酸都与核酸杂交时,增强子寡核苷酸位于检测寡核苷酸探针的3’方向;以及
d)在第一个区段和第二个区段之间存在单个碱基缺口,使得当寡核苷酸探针和增强子寡核苷酸探针都与核酸杂交时,在寡核苷酸之间存在单个碱基缺口;
2)使用当检测探针与核酸杂交时将从检测探针的3’末端裂解可检测标记以释放游离的可检测标记的内切核酸酶来处理核酸;以及
3)测量游离的可检测标记,其中游离的可检测标记的存在表明检测探针与核酸的第一个区段特异性杂交,并表明多态性位点的序列与检测探针互补。
在具体的实施方案中,核酸的拷贝通过聚合酶链反应(PCR)扩增来提供。在另一个实施方案中,被测定的易感性是增加的易感性。在另一个实施方案中,被测定的易感性是降低的易感性。
本发明的另一方面涉及用于评估个体对预防和/或缓解与2型糖尿病相关的症状的治疗药剂的反应的可能性的方法,包括:在从个体获得的核酸样品中确定至少一个多态性标记物的至少一个等位基因的存在或不存在,其中至少一个多态性标记物选自rs2497304(SEQ ID NO:16)、rs947591(SEQ ID NO:30)、rs10882091(SEQ ID NO:4)、rs7914814(SEQ ID NO:24)、rs6583830(SEQ ID NO:20)、rs2421943(SEQ IDNO:15)、rs6583826(SEQ ID NO:19)、rs7752906(SEQ ID NO:32)、rs1569699(SEQ ID NO:6)、rs7756992(SEQ ID NO:21)、rs9350271(SEQ ID NO:33)、rs9356744(SEQ ID NO:34)、rs9368222(SEQ IDNO:35)、rs10440833(SEQ ID NO:36)、rs6931514(SEQ ID NO:37)、rs1860316(SEQ ID NO:10)、rs1981647(SEQ ID NO:11)、rs1843622(SEQ ID NO:9)、rs2191113(SEQ ID NO:13)、rs9890889(SEQ IDNO:31),以及与其连锁不平衡的标记物,其中至少一个标记物的至少一个等位基因的存在的确定,表明了对2型糖尿病治疗药剂发生阳性反应的可能性。在一个实施方案中,2型糖尿病治疗药剂选自药剂表1和药剂表2中显示的药剂。
本发明的另一方面涉及预测被诊断患有2型糖尿病的个体的预后的方法,该方法包括确定从个体获得的核酸样品中至少一个多态性标记物的至少一个等位基因的存在或不存在,其中至少一个多态性标记物选自rs2497304(SEQ ID NO:16)、rs947591(SEQ ID NO:30)、rs10882091(SEQ ID NO:4)、rs7914814(SEQ ID NO:24)、rs6583830(SEQ ID NO:20)、rs2421943(SEQ ID NO:15)、rs6583826(SEQ IDNO:19)、rs7752906(SEQ ID NO:32)、rs1569699(SEQ ID NO:6)、rs7756992(SEQ ID NO:21)、rs9350271(SEQ ID NO:33)、rs9356744(SEQ ID NO:34)、rs9368222(SEQ ID NO:35)、rs10440833(SEQ IDNO:36)、rs6931514(SEQ ID NO:37)、rs 1860316(SEQ ID NO:10)、rs1981647(SEQ ID NO:11)、rs1843622(SEQ ID NO:9)、rs2191113(SEQ ID NO:13)、rs9890889(SEQ ID NO:31),以及与其连锁不平衡的标记物,其中至少一个等位基因的存在的确定,表明了个体中2型糖尿病的更糟的预后。
本发明的另一方面涉及监测经历2型糖尿病治疗的个体的治疗进展的方法,方法包括确定从个体获得的核酸样品中至少一个多态性标记物的至少一个等位基因的存在或不存在,其中至少一个多态性标记物选自rs2497304(SEQ ID NO:16)、rs947591(SEQ ID NO:30)、rs10882091(SEQ ID NO:4)、rs7914814(SEQ ID NO:24)、rs6583830(SEQ ID NO:20)、rs2421943(SEQ ID NO:15)、rs6583826(SEQ IDNO:19)、rs7752906(SEQ ID NO:32)、rs1569699(SEQ ID NO:6)、rs7756992(SEQ ID NO:21)、rs9350271(SEQ ID NO:33)、rs9356744(SEQ ID NO:34)、rs9368222(SEQ ID NO:35)、rs10440833(SEQ IDNO:36)、rs6931514(SEQ ID NO:37)、rs1860316(SEQ ID NO:10)、rs1981647(SEQ ID NO:11)、rs1843622(SEQ ID NO:9)、rs2191113(SEQ ID NO:13)、rs9890889(SEQ ID NO:31),以及与其连锁不平衡的标记物,其中至少一个等位基因的存在的确定,表明了个体的治疗结果。
在一个实施方案中,方法还包括评估个体的样品中的至少一种生物标记物。在另一个实施方案中,方法还包括分析个体的非遗传信息以进行风险评估、诊断或预后。在一个实施方案中,非遗传信息选自年龄、性别、种族、社会经济状态、以前的疾病诊断、对象的医学史、2型糖尿病的家族史、生物化学测量和临床测量。在特别优选的实施方案中,还使用了包括计算总风险的另一个步骤。
本发明还涉及用于在人类个体中评估2型糖尿病易感性的试剂盒,试剂盒含有选择性检测个体的基因组中至少一个多态性标记物的至少一个等位基因的存在或不存在的试剂,其中多态性标记物选自其序列显示在SEQ IN NO:1、SEQ IN NO:2和SEQ IN NO:3中的核酸区段中的多态性标记物,以及与其连锁不平衡的标记物,其中至少一个等位基因的存在表明了对2型糖尿病的易感性。
在一个实施方案中,至少一个多态性标记物选自表10-12中显示的标记物,以及与其连锁不平衡的标记物。在另一个实施方案中,至少一个多态性标记物选自表10-12和表14中显示的标记物,以及与其连锁不平衡的标记物。在另一个实施方案中,至少一个多态性标记物选自rs2497304(SEQ ID NO:16)、rs947591(SEQ ID NO:30)、rs10882091(SEQ ID NO:4)、rs7914814(SEQ ID NO:24)、rs6583830(SEQ IDNO:20)、rs2421943(SEQ ID NO:15)、rs6583826(SEQ ID NO:19)、rs7752906(SEQ ID NO:32)、rs1569699(SEQ ID NO:6)、rs7756992(SEQ ID NO:21)、rs9350271(SEQ ID NO:33)、rs9356744(SEQ IDNO:34)、rs9368222(SEQ ID NO:35)、rs10440833(SEQ ID NO:36)、rs6931514(SEQ ID NO:37)、rs1860316(SEQ ID NO:10)、rs1981647(SEQ ID NO:11)、rs1843622(SEQ ID NO:9)、rs2191113(SEQ IDNO:13)、rs9890889(SEQ ID NO:31),以及与其连锁不平衡的标记物。在另一个实施方案中,至少一个多态性标记物选自rs2497304(SEQID NO:16)、rs947591(SEQ ID NO:30)、rs10882091(SEQ ID NO:4)、rs7914814(SEQ ID NO:24)、rs6583830(SEQ ID NO:20)、rs2421943(SEQ ID NO:15)、rs6583826(SEQ ID NO:19)、rs7752906(SEQ IDNO:32)、rs1569699(SEQ ID NO:6)、rs7756992(SEQ ID NO:21)、rs9350271(SEQ ID NO:33)、rs9356744(SEQ ID NO:34)、rs9368222(SEQ ID NO:35)、rs10440833(SEQ ID NO:36)、rs6931514(SEQID NO:37)、rs1860316(SEQ ID NO:10)、rs1981647(SEQ ID NO:11)、rs1843622(SEQ ID NO:9)、rs2191113(SEQ ID NO:13)、rs9890889(SEQ ID NO:31)。
在一个实施方案中,试剂含有至少一个与个体的基因组的含有至少一个多态性标记物的区段杂交的连续的寡核苷酸、缓冲液和可检测标记。在一个实施方案中,试剂含有至少一对与从对象获得的基因组核酸区段的反向链杂交的寡核苷酸,其中每个寡核苷酸引物对被设计成选择性扩增个体的基因组中包含一个多态性标记物的区段,其中区段的大小为至少30个碱基对。在优选实施方案中,至少一个寡核苷酸与个体的基因组完全互补。在另一个实施方案中,至少一个寡核苷酸可以包含至少一个与个体的基因组的误配。在一个实施方案中,寡核苷酸的长度为大约18到大约50个核苷酸。在另一个实施方案中,寡核苷酸的长度为20-30个核苷酸。
在一个优选实施方案中,试剂盒含有:
长度为5-100个核苷酸的检测寡核苷酸探针;长度为5-100个核苷酸的增强子寡核苷酸探针;以及内切核酸酶;
其中检测寡核苷酸探针与其核苷酸序列在SEQ ID NO:1、SEQ IDNO:2或SEQ ID NO:3中给出核酸的含有至少一个多态性位点的第一个区段特异性杂交;并且其中检测寡核苷酸探针在其3’末端含有可检测标记,在其5’末端含有淬灭基团;其中增强子寡核苷酸长度为5-100个核苷酸,并与相对于寡核苷酸探针5’方向的核苷酸序列的第二个区段互补,以便当两个寡核苷酸都与核酸杂交时,增强子寡核苷酸位于检测寡核苷酸探针的3’方向;其中在第一个区段和第二个片段区段存在单个碱基缺口,使得当寡核苷酸探针和增强子寡核苷酸探针都与核酸杂交时,在寡核苷酸之间存在单个碱基缺口;并且其中当检测探针与核酸杂交时,使用内切核酸酶处理核酸将从检测探针的3’末端裂解可检测标记,以释放游离的可检测标记。
本发明的另一方面涉及寡核苷酸探针在制造用于在人类个体中诊断和/或评估2型糖尿病易感性的诊断试剂中的应用,其中探针与其核苷酸序列在SEQ ID NO:1、SEQ ID NO:2或SEQ ID NO:3中给出核酸的含有至少一个多态性位点的区段杂交,其中片段的长度为15-500个核苷酸。在一个实施方案中,多态性位点选自多态性标记物rs2497304(SEQ ID NO:16)、rs947591(SEQ ID NO:30)、rs10882091(SEQ IDNO:4)、rs7914814(SEQ ID NO:24)、rs6583830(SEQ ID NO:20)、rs2421943(SEQ ID NO:15)、rs6583826(SEQ ID NO:19)、rs7752906(SEQ ID NO:32)、rs1569699(SEQ ID NO:6)、rs7756992(SEQ IDNO:21)、rs9350271(SEQ ID NO:33)、rs9356744(SEQ ID NO:34)、rs9368222(SEQ ID NO:35)、rs10440833(SEQ ID NO:36)、rs6931514(SEQ ID NO:37)、rs1860316(SEQ ID NO:10)、rs 1981647(SEQ IDNO:11)、rs1843622(SEQ ID NO:9)、rs2191113(SEQ ID NO:13)、rs9890889(SEQ ID NO:31),以及与其连锁不平衡的多态性。
本发明的另一方面涉及计算机可读介质,其中储存有:至少一个多态性标记物的识别符;该至少一个多态性标记物的至少一个等位基因在多个被诊断患有2型糖尿病的个体中的频率的指示符;以及该至少一个多态性标记物的至少一个等位基因在多个参比个体中的频率的指示符;其中至少一个多态性标记物选自多态性标记物rs2497304(SEQID NO:16)、rs947591(SEQ ID NO:30)、rs10882091(SEQ ID NO:4)、rs7914814(SEQ ID NO:24)、rs6583830(SEQ ID NO:20)、rs2421943(SEQ ID NO:15)、rs6583826(SEQ ID NO:19)、rs7752906(SEQ IDNO:32)、rs1569699(SEQ ID NO:6)、rs7756992(SEQ ID NO:21)、rs9350271(SEQ ID NO:33)、rs9356744(SEQ ID NO:34)、rs9368222(SEQ ID NO:35)、rs10440833(SEQ ID NO:36)、rs6931514(SEQID NO:37)、rs1860316(SEQ ID NO:10)、rs1981647(SEQ ID NO:11)、rs1843622(SEQ ID NO:9)、rs2191113(SEQ ID NO:13)、rs9890889(SEQ ID NO:31),以及与其连锁不平衡的多态性。在一个实施方案中,连锁不平衡被定义为数值r2为至少0.2和/或|D′|为至少0.8。
在一个实施方案中,包含了关于多个个体的血统的信息。在另一个实施方案中,被诊断患有2型糖尿病的多个个体和多个参比个体具有特定的血统。
另一方面涉及了用于在人类个体中测定2型糖尿病的遗传指示符的装置,包含:计算机可读存储器:以及储存在计算机可读存储器中的例行程序;其中例行程序适用于在处理器上执行,以分析至少一个人类个体的至少一个多态性标记物的标记物和/或单倍型信息,其中该至少一个多态性标记物选自标记物rs2497304(SEQ ID NO:16)、rs947591(SEQ ID NO:30)、rs10882091(SEQ ID NO:4)、rs7914814(SEQ ID NO:24)、rs6583830(SEQ ID NO:20)、rs2421943(SEQ IDNO:15)、rs6583826(SEQ ID NO:19)、rs7752906(SEQ ID NO:32)、rs1569699(SEQ ID NO:6)、rs7756992(SEQ ID NO:21)、rs9350271(SEQ ID NO:33)、rs9356744(SEQ ID NO:34)、rs9368222(SEQ IDNO:35)、rs 10440833(SEQ ID NO:36)、rs6931514(SEQ ID NO:37)、rs 1860316(SEQ ID NO:10)、rs 1981647(SEQ ID NO:11)、rs1843622(SEQ ID NO:9)、rs2191113(SEQ ID NO:13)、rs9890889(SEQ IDNO:31),以及与其连锁不平衡的标记物,该连锁不平衡被定义为数值r2为至少0.2和/或|D′|的值为至少0.8,并根据标记物或单倍型信息产生输出,其中输出包括作为人类个体的2型糖尿病的遗传指示符的至少一个标记物或单倍型的风险测量。
在一个实施方案中,例行程序还包含多个被诊断患有2型糖尿病的个体中至少一个多态性标记物的至少一个等位基因或至少一个单倍型的频率的指示符,以及多个参比个体中至少一个多态性标记物的至少一个等位基因或至少一个单倍型的频率的指示符,其中风险测量是基于人类个体的至少一个标记物和/或单倍型状态与多个被诊断患有2型糖尿病的个体的至少一个标记物和/或单倍型信息的频率指示符的比较。
在本发明的方法、应用、装置或试剂盒的某些实施方案中,连锁不平衡的特征为数值|D′|大于0.8和/或r2大于0.2。但是,在其它实施方案中,其它的r2和|D′|的测量值也是可能的,正如在本文中进一步详细描述的,这样的实施方案也在本发明要求保护的范围内。
在本发明的方法、应用、装置或试剂盒的某些其它实施方案中,个体具有特定的人类血统。在一个实施方案中,血统选自非洲黑人血统、高加索人血统和中国人血统。在另一个实施方案中,血统是非洲黑人血统。在另一个实施方案中,血统是欧洲人血统。在另一个实施方案中,血统是高加索人血统。在某些实施方案中,血统是经历遗传分析或基因分型的个体自己报告的。在其它实施方案中,血统是通过包括在个体的核酸样品中检测至少一个多态性标记物的至少一个等位基因的遗传测定来确定的,其中等位基因的存在或不存在是个体血统的指示。
在本发明的方法、应用、装置或试剂盒的其它具体实施方案中,个体是肥胖的。在其它实施方案中,个体是不肥胖的。在一个实施方案中,肥胖通过BMI(身体质量指数)值大于25来确定。在另一个实施方案中,肥胖通过BMI值大于30来确定。BMI的其它整数或分数截止值也是可能的,并在本发明的范围内,包括但不限于BMI大于23、24、25.5、26、26.5、27、27.5等。在一个实施方案中,不肥胖的个体被定义为所有不符合肥胖的BMI标准的个体。在其它实施方案中,不肥胖的个体是具有特定BMI截止值的个体,例如BMI小于25、小于24、小于23、小于22、小于21或小于20。非整数的BMI截止值也可用于定义不肥胖的个体。一般来说,肥胖和不肥胖的组在它们的BMI值方面不重叠。在某些实施方案中,用于定义组的截止值是相同的,例如BMI大于或小于25。在其它实施方案中,使用不同的截止值,例如肥胖个体使用大于27,不肥胖个体使用小于23。所有相关的适合于定义肥胖和不肥胖个体的BMI的范围也是可能的,并在本发明的范围内。
附图说明
从下面对本发明的优选实施方案的更具体的描述,本发明的上述以及其它的目标、特点和优点将变得明显。
图1显示了在含有与2型糖尿病相关联的标记物的染色体6p22.3区域中的连锁不平衡模式图,(a)X轴显示了相对于NCBI Build 35基因组装配图(与Build 36相同)的位置,Y轴显示了区域中的连锁不平衡测量值。CDKAL1基因的跨度用箭头指示,外显子的位置用垂直于对角线的黑色条指示。SNP标记物被等距离作图,而不是按照它们的物理位置作图。图显示了连锁不平衡的r2测量值,其中阴影部分与标记物之间的r2的两两值成比例。(b)CDKAL基因的5′末端的特写,显示了LD区块C06区域(SEQ ID NO:1),在其中已经发现了几个与2型糖尿病相关联的标记物。几个有关联的SNP标记物的位置在图上标明了。
图2显示了在含有与2型糖尿病相关联的标记物的染色体10q23.33区域中的连锁不平衡。X轴显示了相对于NCBI Build 35基因组装配图的位置,Y轴显示了区域中的连锁不平衡测量值。标出了四个有关联的SNP标记物rs2497304、rs947591、rs10882091和rs7914814的位置,以及在LD区块中的三个基因IDE、KIF11和HHEX的跨度和外显子。本图显示了连锁不平衡的r2测量值,其中阴影部分与标记物之间的r2的两两值成比例。
图3显示了在不肥胖的和所有患者中在含有与糖尿病相关联的标记物的染色体17q24.3区域中的连锁不平衡。标出了5个SNP标记物rs 1860316、rs1981647、rs1843622、rs2191113和rs9890889的位置。本图显示了连锁不平衡的r2测量值,其中阴影部分与标记物之间的r2的两两值成比例。
图4显示了来自单个SNPs和双标记物单倍型的分析的653,025个调整的x2统计学数据(圆圈)的Q-Q作图。出于参比的目的在图中包含了等角线(黑线)。水平的虚线表示假设对测试的653,025个SNPs/单倍型和三个基因型进行Bonferroni校正时基因组范围的显著性的阈值。
图5显示了T2D与6p22.3关联的示意图。a)6号染色体上在1Mb的间距内(20.5-21.5Mb,NCBI Build 34)的两两关联结构。上方的图包含了来自于HapMap release 19的CEU人群的1047个常见SNPs(MAF>5%)的两两的D′,而下方的图包含了同样的SNPs组的两两的r2值。b)根据HapMap数据组(Nature 437,1299-1320(2005年10月27日)),在该间距中重组热点的位置。c)作图于该间距中的两个基因E2F3和CDKAL1的外显子的位置(垂直的条)。d)分别为所有T2D病例(黑点)、不肥胖的T2D病例(空心圆圈)和肥胖的T2D病例(空心三角形)在该间距中的基因组范围内的关联性结果的示意图。标绘的是-logP,其中P是针对标记物的染色体位置调整过的P值。所有四张图都使用同样的水平Mb标尺,显示在图d)的底部。
图6显示了来自INS-1细胞的CDKAL1 cDNA。第1和第2道分别含有从外显子2到8和外显子7到13扩增的CDKAL1 cDNA,给出的条带的大小分别为596bp和738bp。第3道中的β-肌动蛋白用作阳性对照(837bp),第4道是没有引物的阴性对照反应。大小的标准在左边给出。
图7显示了rs7756992和rs13266634与胰岛素分泌的关联性。对于两个SNPs——rs7756992和rs13266634的三种不同的基因型,通过校正的胰岛素反应(参见方法)估算了平均的对数变形的胰岛素分泌水平。显示了来自进行了口服葡萄糖耐受测试的丹麦Inter99研究的3982个个体(231例T2D病例和3751个对照)的结果。个体的数量包含在每个柱下方,标准误差(s.e.m.)用水平的条来表示。包含的P值来自对数变形的胰岛素分泌水平对基因型状态的回归,根据年龄、性别和患病状态进行了调整,假设了相加模型(Padd)或回归模型(Prec)。
图8进一步显示了rs7756992和rs13266634与胰岛素分泌的关联性。a)平均的对数变形的胰岛素分泌水平,对于SNP rs7756992的三种不同的基因型通过校正的胰岛素反应(CIR)来估算。对来自进行了OGTT的丹麦Inter99群体(223例T2D病例和3715个对照)的3938个个体的组进行了胰岛素分泌水平的估算。结果为所有个体(最左边的条)、男性(中间的条)和女性(最右边的条)进行了分别的显示。每个估算后面的个体的数量与相应的基因型一起显示在柱下方的括号中。平均值的标准误差用每个柱顶部上的条显示。b)对3926个个体(228例T2D病例和3698个对照)的SNP rs13266634的不同基因型进行的相应的估算。
发明详述
下面描述了本发明的优选实施方案。
本发明公开了已经被发现与2型糖尿病相关联的多态性标记物和单倍型。在某些多态性SNP标记物上的特定等位基因以及含有这些等位基因的单倍型已经被发现与2型糖尿病有关。正如在本文中进一步详细描述的,这样的标记物和单倍型可用于评估对2型糖尿病的易感性。本发明的其它应用包括利用本发明的多态性标记物评估对2型糖尿病治疗药剂的反应的方法,以及用于评估个体对2型糖尿病的易感性的试剂盒。
定义
在本发明的文本中,下面的术语将具有以下所指的含义:
本文描述的“多态性标记物”、有时被称为“标记物”,是指遗传多态性位点。每种多态性标记物具有至少两个序列变体,其特征为多态性位点上的特定等位基因。因此,与多态性标记物的遗传关联表明存在与该具体多态性标记物的至少一个特定等位基因的关联。标记物可以含有在基因组中发现的任何变体类型的任何等位基因,包括SNPs、微卫星、插入、缺失、重复和易位。
“等位基因”是指染色体上给定位点(位置)的核苷酸序列。因此,多态性标记物等位基因是指染色体上标记物的组成(即序列)。来自个体的基因组DNA对于任何给定的多态性标记物来说含有两个等位基因,代表了每条染色体上标记物的每个拷贝。在本文中使用的核苷酸的序列编码是A=1、C=2、G=3、T=4。
本文描述的序列的共核苷酸多义性(conucleotide ambiguity)由IUPAC-IUB提出。这些编码与EMBL、GenBank和PIR数据库使用的编码相一致。
IUB | 含义 |
A | 腺嘌呤 |
C | 胞嘧啶 |
G | 鸟嘌呤 |
T | 胸腺嘧啶 |
R | G或A |
Y | T或C |
K | G或T |
M | A或C |
S | G或C |
W | A或T |
B | C、G或T |
D | A、G或T |
H | A、C或T |
V | A、C或G |
N | A、C、G或T(任一碱基) |
在本文中,在群体中(自然群体或合成的群体,例如合成分子的文库)其上可能存在一种以上序列的核苷酸位置被称为“多态性位点”。
“单核苷酸多态性”或“SNP”是当基因组中特定位置上的单个核苷酸在物种的成员之间或一个个体中成对的染色体之间不同时发生的DNA序列变异。大多数SNP多态性具有两个等位基因。在这种情况下,每个个体或者是多态性的一个等位基因的纯合子(即个体的两个染色体拷贝在SNP位置处具有同样的核苷酸),或者个体是杂合子(即个体的两条姊妹染色体含有不同的核苷酸)。在本文中报告的SNP的命名是指官方的参考SNP(rs)ID身份标签,是由国家生物技术信息中心(NCBI)为每个独特的SNP指定的。
本文描述的“变体”是指与参比DNA不同的DNA区段。“标记物”或“多态性标记物”按照本文的定义,是变体。与参比不同的等位基因被称为“变体”等位基因。
“微卫星”是在特定位点具有多个小的长度为2-8个核苷酸的碱基重复(例如CA重复)的多态性标记物,其中重复长度的数量在总群体中是变化的。“indel”是常见的多态性形式,含有一般只有几个核苷酸长的小的插入或缺失。
本文描述的“单倍型”是指基因组DNA的一个区段,其特征为沿着区段排列有等位基因的特定组合。对于二倍体生物例如人类来说,单倍型含有每个多态性标记物或位点的等位基因对的一个成员。在某些实施方案中,单倍型可以含有两个或两个以上等位基因、三个或三个以上等位基因、四个或四个以上等位基因、或五个或五个以上等位基因。在本文中,单倍型被描述在标记物名称和该单倍型中标记物的等位基因的上下文中,因为单倍型例如“3rs7758851”是指单倍型中存在标记物rs7758851的等位基因3,等价于“rs7758851等位基因3”。此外,单倍型中的等位基因编码与单个标记物使用的相同,即1=A、2=C、3=G和4=T。
本文描述的术语“易感性”包含了增加的易感性和降低的易感性。因此,本发明的多态性标记物的特定等位基因和/或单倍型的特征可以是2型糖尿病的增加的易感性(即增加的风险),被表征为对于特定等位基因或单倍型而言,相对风险(RR)或比值比(OR)大于1。或者,本发明的标记物和/或单倍型的特征可以是2型糖尿病的降低的易感性(即降低的风险),被表征为相对风险小于1。
“核酸样品”是从个体获得的含有核酸的样品。在某些实施方案、即在特定多态性标记物和/或单倍型的检测中,核酸样品包含基因组DNA。这样的核酸样品可以从任何含有基因组DNA的来源获得,包括例如血液样品、羊水样品、脑脊液样品,或来自皮肤、肌肉、口腔或结膜粘膜、胎盘、胃肠道或其它器官的组织样品。
术语“2型糖尿病治疗药剂”是指可用于缓解或预防与2型糖尿病相关的症状的药剂。
本文中描述的术语“与2型糖尿病相关的核酸”是指已经被发现与2型糖尿病相关的核酸。这包括但不限于本文描述的标记物和单倍型,以及与其强烈连锁不平衡(LD)的标记物和单倍型。在一个实施方案中,与2型糖尿病相关的核酸是指通过LD区块中的至少一个多态性标记物被发现与2型糖尿病相关的LD区块。
本文描述的术语“不肥胖的”是指个体具有低于预定阈值的计算出的身体质量指标(BMI),例如阈值为30或以下。正如在本文中更详细描述的,其它可用于定义术语的阈值也是可能的。计算BMI的公式为[体重(单位为公斤)]/[身高(单位为米)]2。术语“肥胖的”是指个体的BMI高于预定的阈值,例如阈值为30。
本文描述的术语“LD区块C06”是指6号染色体上位于标记物rs4429936和rs6908425之间的连锁不平衡(LD)区块,对应于NCBI(国立生物技术信息中心)Build 35中的20,634,996-20,836,710的位置(SEQ ID NO:1)。
本文描述的术语“LD区块C10”是指10号染色体上位于标记物rs2798253和rs11187152之间的连锁不平衡(LD)区块,对应于NCBI(国立生物技术信息中心)Build 35中的94,192,885-94,490,091的位置(SEQ ID NO:2)。
本文描述的术语“LD区块C17”是指17号染色体上位于标记物rs11077501和rs4793497之间的连锁不平衡(LD)区块,对应于NCBI(国立生物技术信息中心)Build 35中的66,037,656-66,163,076的位置(SEQ ID NO:3)。
本文描述的术语“CDKAL1”是指类CDK5调节亚基相关蛋白1的基因1,它在人类基因组的NCBI Build 35中跨越20,642,736-21,340,611的位置。
本文描述的术语“SLC30A8”是指溶质载体家族30的8号成员的基因。该基因位于8号染色体上,其最长的同种型在人类基因组装配图NCBI Build 36中118,032,398到118,258,134之间的位置,跨度长达225kb,NCBI Build 36中118,032,398到118,258,134分别对应于在NCBI Build 34中117,919,805和118,145,541的位置。在这两种版本中,该基因都跨越225,736bp的基因组序列。
通过使用可用于在基因组中同时测量超过300,000个SNPs的Illumina 330K芯片对冰岛2型糖尿病患者和群体对照个体进行基因分型,本发明鉴定到了大量与2型糖尿病有关联的变体。使用单个SNPs、双标记物单倍型和延伸的连锁不平衡(LD区块)区域中的延长的单倍型,在基因组范围内进行了关联性分析。在根据亲缘性校正了p-值后,最初在21个位点上(即基因组中的遗传易感性位置)鉴定了49个单标记物和双标记物单倍型,它们的p-值小于5x10-5(表1)。此外,通过同样的标准选择了位于8个其它位点上的10个延长的单倍型(表2)。在患者组中,700个个体是不肥胖的(BMK<30),它们的关联性被单独测试。在根据亲缘性校正了p-值后,在20个位点上鉴定了36个单标记物和双标记物单倍型,它们的p-值小于5x10-5(表3)。这些位点中有3个在对整个组进行分析时也被鉴定到了。此外,通过同样的标准选择了位于4个其它位点上的6个延长的单倍型(表4)。531位患者的肥胖组(BMI>30)的关联性也被单独分析。在根据亲缘性校正了p-值后,在16个位点上鉴定了38个单标记物和双标记物单倍型,它们的p-值小于5x10-5(表5)。这些位点中有1个在对整个组进行分析时也被鉴定到了,但是使用该标准,在不肥胖组和肥胖组之间没有发现重叠。此外,位于7个其它位点上的10个延长的单倍型在肥胖型糖尿病的关联性分析中具有小于5x10-5的p-值(表6)。
这些单标记物关联性和双标记物和延长的单倍型关联性的结果,为2型糖尿病的多个易感性变体提供了证据。应该指出,对于本文显示的单标记物SNP分析来说,易感性变体可以表现为增加的风险,其中与对照相比,一个等位基因在患者组中出现过多。或者,易感性变体可以表现为正在讨论的SNP的另一个等位基因,对于该等位基因来说,与对照组相比,预计在患者中将出现较少。这是对含有两个等位基因的遗传元件进行关联性分析的自然结果。对于多标记物单倍型或含有一个以上标记物的多态性标记物来说,可以观察到与一个(或多个)风险等位基因或单倍型的风险关联性。依赖于正在讨论的遗传区域中的遗传成分和单倍型结构,也可以观察到与一个(或多个)保护性变体或单倍型(RR值小于整体)相关联的形式的保护性变体。
通过用两个单标记物(rs1569699和rs7756992)和三个双标记物单倍型对染色体6p22.3作图(3 rs7758851 2 rs1569699,1 rs47125273rs7756992,1 rs7756992 3 rs9295478;参见表3),鉴定到了最显著的关联性信号之一。这些标记物位于6号染色体的20634996和20836710位置之间的延伸的LD(LD区块)区域中(NCBI Build 35;SEQ ID NO:1),在标记物rs4429936和rs6908425之间(图1)。该区域含有类CDK5调节亚基相关蛋白1的基因1(NM_017774)的5′末端,包含外显子1-5。在两个其它的2型糖尿病群体中,这些标记物的关联性得到了证实(参见表7)。
对rs7756992等位基因G与2型糖尿病的增加的风险的关联性的跟踪研究,在欧洲人血统的个体中(等位基因特异性比值比(OR)=1.16;P=3.9x10-11),以及来自香港的汉族中国人血统的个体中(OR=1.25;P=0.00018)建立起了标记物与2型糖尿病的关联性(参见表14、15和17)。其它在欧洲人和中国人群体中已经被显示与2型糖尿病相关的、在LD的LD区块C06(SEQ ID NO:1)中与rs7756992连锁不平衡的变体包括rs1569699、rs7752906、rs9350271、rs9356744、rs9368222、rs10440833和rs6931514(表18)。rs77566992等位基因G变体的基因型比值比支持了遗传的近似隐性的状态(表20)。具体来说,在欧洲人和香港组中,纯合子的OR分别为1.45和1.55。已经发现rs77566992等位基因G风险变体与携带者中降低的胰岛素反应有关联(表21,图7和8)。已经发现变体的纯合携带者与杂合子或非携带者相比胰岛素反应估计降低了24%,表明该变体通过降低胰岛素的分泌而赋予了T2D的风险。因此,rs7756992标记物以及与其连锁不平衡的标记物(包括但不限于rs1569699、rs7752906、rs9350271、rs9356744、rs9368222、rs10440833和rs6931514),可用于在个体中评估对2型糖尿病的增加的易感性。
CDKAL1基因产物的功能是未知的。但是,正如基因的名字所暗示的,蛋白产物与另一种蛋白CDK5调节亚基相关蛋白1(CDK5RAP1)类似。CDK5RAP1在神经元组织中表达,在那里它通过结合CDK5调节亚基p35抑制周期蛋白依赖性激酶5(CDK5)的活性(Ching,Y.P.,Pang,A.S.,Lam,W.H.,Qi,R.Z.&Wang,J.H.J Biol Chem 277,15237-40(2002))。在胰腺β细胞中,已经显示出在糖毒性条件下CDK5在β细胞功能的丧失中扮演了重要角色(Wei,F.Y.等,Nat Med 11,1104-8(2005))。此外,对CDK5/p35复合物的抑制阻止了由糖毒性产生的胰岛素基因表达的降低(Ubeda,M.,Rukstalis,J.M.&Habener,J.F.J Biol Chem 281,28858-64(2006))。CDKAL1在胰腺β细胞中CDK5/p35的抑制中发挥的作用可能与CDK5RAP1在神经元组织中的作用相似。因此,CDKAL1表达的降低或抑制功能的降低可能导致对糖毒性反应的受损。提出的数据显示出CDKAL1在大鼠的胰腺β细胞株INS-1中得到了表达(图6)。
根据CDKAL1的预测的功能和SLC30A8的已知的功能,我们可以预计rs7756992和rs13266634都能影响胰岛素分泌。为了评估两个SNPs对胰岛素分泌的影响,我们在经历了口服葡萄糖耐受测试(OGTT)的来自Inter99研究(部分丹麦人B)的一组个体中分析了基因型状态对校正的胰岛素反应(CIR)的影响。对于rs7756992来说,我们证明了风险等位基因的纯合携带者具有比杂合携带者或非携带者低大约24%的CIR(P<0.00001,图7)。这个观察与变体的疾病风险的近似隐性状态的遗传相符。此外,在男性和女性(图8)以及T2D患者和对照中都显示了对CIR的影响,并且对BMI状态的调整不影响结果(表21)。rs13266634对胰岛素反应的影响较小但是显著,对于该风险变体来说,CIR的降低符合加合效应。对于每种变体来说,没有观察到对胰岛素灵敏度的影响(表21)。
将CDKAL1鉴定为T2D的易感性基因,为遗传因素如何能够造成对T2D的易感的拼图增加了新的一片。尽管该基因的功能仍有待阐明,但我们已经显示出它在胰腺β细胞中表达,并且基因中的变异与胰岛素分泌相关。与CDK5RAP1的相似性进一步表明,CDKAL1在糖毒性条件下可以通过与CDK5相互作用促进胰岛素的产生。总之,我们在CDKAL1基因中鉴定到了以近似隐性的方式钝化胰岛素反应并造成对T2D易感的变体。
本发明在染色体10q23.33区域中鉴定到了与2型糖尿病相关联的7个单标记物和7个双标记物单倍型(表1)。当肥胖患者被单独分析时,大多数这些标记物也与糖尿病相关,具有升高的RR值(表5)。这些标记物位于94192885和94490091位置(NCBI Build 35)之间的一个LD区块中,对应于标记物rs2798253和rs11187152之间的基因组区段(图2)。该LD区块含有三个基因:胰岛素降解酶(IDE)(NM_004969)、驱动蛋白家族11号成员(KIF11)(NM_004523)和造血表达同源异形盒(HHEX)(NM_002729)。
IDE可能属于负责细胞内肽信号转导的蛋白酶家族。尽管它在胰岛素的细胞加工中的作用还没有被确定,但胰岛素降解酶被认为参与了胰岛素反应的终止(Fakhrai-Rad等,Human Molecular Genetics9:2149-2158,2000)。糖尿病GK大鼠的遗传分析已经发现,在GK等位基因中,在IDE基因中的两个氨基酸取代(H18R和A890V),使转染的细胞中胰岛素降解活性降低了31%。但是,当对H18R和A890V变体分别进行研究时,没有观察到影响,表明两个变体对胰岛素降解具有协同效应。在细胞裂解液中没有观察到对胰岛素降解的影响,表明影响可能与胰岛素的受体介导的内化作用相偶联。带有IDE GK等位基因的同类系大鼠显示出了餐后高血糖,脂肪细胞中的低脂肪生成,钝化的胰岛素刺激的葡萄糖跨膜摄取,以及在分离的肌肉中降低的胰岛素降解。对其它大鼠株系的分析证实了功能障碍的IDE等位基因是GK大鼠所独有的。作者得出结论,在GK大鼠中,IDE在糖尿病表现型中扮演了重要角色。IDE作为2型糖尿病的候选基因在人类中的研究得到了不一致的结果。最近,两项大的研究,通过突变筛选和使用标签SNPs在基因上进行的单倍型分析,分析了IDE与2型糖尿病的关联性(Groves等,Diabetes 52:1300-1305,2003;Florez等,Diabetes55:128-135,2006)。两项研究均得出结论,常见的IDE变体不可能赋予对2型糖尿病的显著的风险。但是,这些研究没有包含在图2中定义的全部LD区块,至少某些在我们的研究中被鉴定为与2型糖尿病相关的标记物,位于上述那些研究所分析的区域之外。基于这里报告的结果,与IDE连锁不平衡的标记物与2型糖尿病有关联,为IDE在2型糖尿病的病因学中的作用提供了遗传证据。
KIF11编码了属于驱动蛋白(kinesin)类蛋白家族的马达蛋白。该蛋白家族的成员已知参与了各种不同种类的纺锤体动力学。该基因产物的功能包括细胞有丝分裂过程中的染色体定位、中心体分离和双极纺锤体的建立。该基因不是糖尿病的好的功能候选物,但是由于其位于相关的LD区块中,不得不被当做位置候选物。
HHEX编码转录因子的同源异形盒家族的成员,它们中的许多参与了发育过程。在特定造血株系中的表达,表明该蛋白可能在造血分化中发挥作用。HHEX对于胰腺发育是必需的;在HHEX阴性小鼠胚胎中,腹侧胰腺的特化完全失败(Bort等,Development 131,797-806,2004)。其它参与胰腺发育的转录因子包括了MODY基因以及其它已被暗示与晚期发作的糖尿病有关的因子。HHEX也是用于心脏诱导的Wnt拮抗剂的基本效应器(Foley和Mercola,GENES & DEVELOPMENT19:387-396,2005)。这将HHEX置于与最近建立的2型糖尿病基因TCF7L2同样的途径中,这些数据合在一起,使HHEX成为2型糖尿病的功能和位置候选物。
在丹麦2型糖尿病病例-对照组中以及来自PENN CATH研究的美国高加索人2型糖尿病组中,rs2497304、rs947591、rs10882091和rs7914814与2型糖尿病的关联性得到了证实(表8)。当将两个组合并时,rs947591的关联性达到了显著性为0.05的水平,在合并的组中风险为1.1。当所有的组合并起来时,对于rs947591标记物来说风险是1.15。这些结果表明,染色体10上的含有IDE和HHEX的LD区块中的变体是2型糖尿病的易感性变体。
此外,在不肥胖患者中,在染色体17q24.3区域中发现了5个单标记物和双标记物单倍型与2型糖尿病有关联(表3)。这些标记物中的某些显示出了表3中报告的最强的关联性,在对所有糖尿病患者进行的分析中也观察到了与该区域的关联性(表1)。这些标记物位于17号染色体上66037656和66163076位置(NCBI Build 35)之间的两个邻近的LD区块中,在标记物rs11077501和rs4793497之间(图3)。在全基因组水平上,这种关联性是显著的。在这些LD区块中没有已知的基因。但是,有可能该区域中的变体影响了邻近区域中的基因,包括KCNJ2和KCNJ16。或者,这些变体可能影响了这些LD区块中的未知基因。
从其它的关联性研究,获得了rs7756992和染色体6p22.3上含有CDKAL1基因(NM_017774)的5′末端、包括外显子1-5的LD区块C06中的相关标记物与2型糖尿病的关联性。在三项大的研究中,与rs7756992高度关联(r2 0.68;D′0.95)的两个相当的标记物rs7754840和rs10946398显示出了与II型糖尿病的显著关联(Saxena,R等,Science2007;316:1331-6;Zeggini,E等,Science 2007;316:1336-41;Scott,U等,Science 2007;316:1341-5)。因此,这些研究进一步支持CDKAL基因与2型糖尿病相关。
rs10882091和染色体10q23.33上的相关标记物与II型糖尿病的关联性也得到了最近的出版物的支持。在四项大的研究中,发现了高度相关的标记物rs1111875(r20.51;D′=1)与II型糖尿病显著关联(Sladek,R等,Nature 2007;445:828-30;Saxena,R等,Science 2007;316:1331-6;Zeggini,E等,Science 2007;316:1336-41;Scott,LJ等,Science 2007;316:1341-5)。因此,最近的研究为本发明人的发现、即本文描述的LD区块C10区域中的标记物是2型糖尿病的风险因子提供了附加的支持。
当在个体间进行比较时,种群中的基因组序列是不相同的。相反,个体间在基因组中的许多位置上表现出了基因组序列变化性。这种序列的变化通常被称为多态性,在每个基因组中有许多这样的位点。例如,人类基因组表现出在平均每500个碱基对就发生序列变异。最常见的序列变异包括基因组中单一碱基位置的碱基变化,这种序列变异或多态性,通常被称为单核苷酸多态性(“SNPs”)。这些SNPs据信是在单个突变事件中发生的,因此通常可能在每个SNP位点存在两个可能的等位基因;原始的等位基因和突变的等位基因。由于自然遗传漂移并可能也由于选择压力,最初的突变产生了多态性,其特征为在任何给定的群体中其等位基因的特定频率。在人类基因组中发现了许多其它类型的序列变异,包括微卫星、插入、缺失、倒置和拷贝数变化。多态性微卫星在特定位点含有多个小的碱基重复(例如CA重复,互补链上的TG重复),重复的长度的数量在总群体中是变化的。概括地说,多态性位点的每个版本的序列都代表了多态性位点的特定等位基因。这些序列变体都可以被称为多态性,它们发生在特定多态性位点,表明了所述序列变体的特征。概括地说,多态性可以包含任何数量的特定等位基因。因此,在本发明的一个实施方案中,多态性的特征为在任何给定群体中存在两种或两种以上等位基因。在另一个实施方案中,多态性的特征为存在三种或三种以上等位基因。在其它实施方案中,多态性的特征为四种或四种以上等位基因、五种或五种以上等位基因、六种或六种以上等位基因、七种或七种以上等位基因、九种或九种以上等位基因或十种或十种以上等位基因。所有这样的多态性都可用于本发明的方法和试剂盒中,因此在本发明的范围内。
在某些情况下,对多态性位点处不同等位基因进行参比而不选择参比等位基因。或者,对于特定多态性位点可以指定参比序列。参比等位基因有时被称为“野生型”等位基因,它通常被选为第一个被测序的等位基因或来自“未受影响的”个体(例如没有显示出性状或疾病表型的个体)的等位基因。
本文指称的SNP标记物的等位基因,根据在使用的SNP分析中出现在多态性位点上的碱基A、C、G或T进行指称。本文使用的SNPs的等位基因编码如下:1=A,2=C,3=G,4=T。但是,本技术领域的专业人员将会认识到,在每种情况下,通过分析或读取相反的DNA链,可以测量互补的等位基因。因此,对于其特征为A/G多态性的多态性位点(多态性标记物)来说,使用的分析方法可以被设计成特异性检测两种可能的碱基即A和G中的一种或两种的存在。或者,通过设计分析方法,使得它被设计成检测DNA模板上的相反链,可以测量互补碱基T和C的存在。从定量来说(例如根据相对风险),从任一条DNA链(+链或-链)测量获得的结果是相同的。
典型情况下,对特定序列指定参比序列。与参比不同的等位基因有时被称为“变体”等位基因。本文使用的变体序列是指与参比序列不同但是基本上相似的序列。本文描述的多态性遗传标记物处的等位基因是变体。其它的变体可以包含影响到多肽的变化。当与参比核苷酸序列进行比较时,序列差异可以包括单个核苷酸或一个以上核苷酸的插入或缺失,从而导致阅读框移码;至少一个核苷酸的变化,导致了所编码的氨基酸的变化;至少一个核苷酸的变化,导致产生了未成熟的终止密码子;几个核苷酸的缺失,导致了核苷酸编码的一个或多个氨基酸的缺失;一个或几个核苷酸的插入,例如通过不对称重组或基因转换,导致了阅读框编码序列的中断;序列的全部或一部分的复制;易位;或核苷酸序列的重排。这样的序列变化可以改变核酸编码的多肽。例如,如果核酸序列中的变化导致了阅读框移码,阅读框移码可以导致编码的氨基酸的变化,和/或导致产生了未成熟的终止密码子,导致产生了截短的多肽。或者,与疾病或性状有关的多态性可以是一个或多个核苷酸中的同义变化(即变化不导致氨基酸序列的变化)。这样的多态性可能例如改变剪接位点、影响mRNA的稳定性或运输,或影响转录或编码的多肽的翻译。也可以改变DNA,以增加在体细胞水平上发生结构变化、例如扩增或缺失的可能性。参比核苷酸序列编码的多肽是具有特定的参比氨基酸序列的“参比”多肽,由变体等位基因编码的多肽被称为具有变异的氨基酸序列的“变体”多肽。
单倍型是指DNA的区段,其特征为沿着区段排列有等位基因的特定组合。对于二倍体生物例如人类来说,单倍型含有每个多态性标记物或位点的一对等位基因中的一个成员。在某些实施方案中,单倍型可以含有两个或两个以上等位基因、三个或三个以上等位基因、四个或四个以上等位基因、或五个或五个以上等位基因,每个等位基因对应于区段上的特定多态性标记物。单倍型可以含有在多态性位点上具有特定等位基因的各种多态性标记物、例如SNPs和微卫星的组合。因此,单倍型含有各种不同遗传标记物等位基因的组合。
特定多态性标记物和/或单倍型的检测可以通过本技术领域已知的检测多态性位点的序列的方法来完成。例如,可以使用用于基因分型的标准技术检测SNPs和/或微卫星标记物的存在,例如基于荧光的技术(Chen,X.等,Genome Res.9(5):492-98(1999)),利用PCR、LCR、巢式PCR以及其它用于核酸扩增的技术。可用于SNP基因分型的具体的方法包括但不限于TaqMan基因分型分析方法和SNPlex平台(Applied Biosystems)、质谱(例如来自Sequenom的MassARRAY系统)、微型测序方法、实时PCR、Bio-Plex系统(BioRad)、CEQ和SNPstream系统(Beckman)、分子倒置探针阵列技术(例如AffymetrixGeneChip)、BeadArray技术(例如Illumina GoldenGate和Infinium分析方法)以及Centaurus分析方法(Nanogen)。通过这些以及其它本技术领域的专业人员可用的方法,可以鉴定出多态性标记物包括微卫星、SNPs或其它类型的多态性标记物的一个或多个等位基因。
在本文描述的某些方法中,对2型糖尿病具有增加的易感性(即增加的风险)的个体,是在其中鉴定到了赋予对2型糖尿病的增加的易感性的一个或多个多态性标记物的至少一个特定等位基因或单倍型(即风险标记物等位基因或单倍型)的个体。在一种情况下,风险标记物或单倍型是赋予了对2型糖尿病的显著增加的风险(或易感性)的标记物或单倍型。在一个实施方案中,与标记物或单倍型相关的显著性通过相对风险(RR)来测量。在另一个实施方案中,与标记物或单倍型相关的显著性通过比值比(OR)来测量。在另一个实施方案中,显著性通过百分比来度量。在一个实施方案中,显著增加的风险被测量为风险(相对风险和/或比值比)为至少1.2,包括但不限于:至少1.2、至少1.3、至少1.4、至少1.5、至少1.6、至少1.7、至少1.8、至少1.9、至少2.0、至少2.5、至少3.0、至少4.0和至少5.0。在具体的实施方案中,至少1.2的风险(相对风险和/或比值比)是显著的。在另一个具体实施方案中,至少1.3的风险是显著的。在另一个实施方案中,至少1.4的风险是显著的。在另一个实施方案中,至少大约1.5的相对风险是显著的。在另一个实施方案中,至少大约1.7的风险的显著增加是显著的。但是,也考虑到了其它的截止值,例如至少1.15、1.25、1.35等,这些截止值也在本发明的范围内。在其它实施方案中,风险的显著增加是至少大约20%,包括但不限于大约25%、30%、35%、40%、45%、50%、55%、60%、65%、70%、75%、80%、85%、90%、95%、100%、150%、200%、300%和500%。在一个具体的实施方案中,风险的显著增加是至少20%。在其它实施方案中,风险的显著增加是至少30%、至少40%、至少50%、至少60%、至少70%、至少80%、至少90%和至少100%。但是,也考虑到了本技术领域的专业技术人员认为适合于表征本发明的其它截止值或范围,它们也在本发明的范围内。
本发明的风险多态性标记物或单倍型,是在具有疾病或性状(受影响的)风险的个体中至少一个标记物的至少一个等位基因或单倍型的出现频率,与它在对比组(对照)中的出现频率相比更高的标记物或单倍型,其中标记物或单倍型的存在表明了对疾病或性状的易感性。在一个实施方案中,对照组可以是群体样品,即来自总群体的随机样品。在另一个实施方案中,对照组由一组没有患病的个体代表。在一个实施方案中,这些没有患病的对照的特征可以是不存在一种或多种与特定疾病相关的症状。在另一个实施方案中,没有患病的对照组的特征为没有一种或多种疾病特异性风险因子。在一个实施方案中,这样的风险因子是至少一种环境风险因子。代表性的环境因子是已知影响或被考虑到影响发生特定疾病或性状的风险的天然产物、矿物质或其它化学物质。其它环境风险因子是与生活方式有关的风险因子,包括但不限于饮食习惯、主要居住地的地理位置和职业性风险因子。在另一个实施方案中,风险因子是至少一种遗传风险因子。
简单的相关性检验的例子是在2x2表格上进行的Fisher-精确检验。给定一组染色体,使用含有两种标记物或单倍型、含有一种标记物或单倍型而不含另一种、以及不含有标记物或单倍型的染色体的数量,构建出2x2表格。
在本发明的另一个实施方案中,对2型糖尿病具有降低的易感性(即降低的风险)的个体,是在其中鉴定到了赋予对2型糖尿病的降低的易感性的一个或多个多态性标记物的至少一个特定等位基因或单倍型的个体。赋予降低的风险的标记物等位基因和/或单倍型也被说成是保护性的。在一种情况下,保护性标记物或单倍型是赋予了对疾病或性状的明显降低的风险(或易感性)的标记物或单倍型。在另一个实施方案中,在来自个体的核酸样品中不存在风险等位基因,也表明了由于缺少风险等位基因而造成的对疾病的保护性。在一个实施方案中,显著降低的风险被测量为相对风险小于0.9,包括但不限于小于0.9、小于0.8、小于0.7、小于0.6、小于0.5、小于0.4、小于0.3、小于0.2和小于0.1。在一个具体的实施方案中,显著降低的风险是小于0.7。在另一个实施方案中,显著降低的风险是小于0.5。在另一个实施方案中,显著降低的风险是小于0.3。在另一个实施方案中,风险(或易感性)的降低是至少20%,包括但不限于至少25%、至少30%、至少35%、至少40%、至少45%、至少50%、至少55%、至少60%、至少65%、至少70%、至少75%、至少80%、至少85%、至少90%、至少95%和至少98%。在一个具体实施方案中,风险的显著降低是至少大约30%。在另一个实施方案中,风险的显著降低是至少大约50%。在另一个实施方案中,风险的显著降低是至少大约70%。但是,也考虑到了本技术领域的专业技术人员认为适合于表征本发明的其它截止值或范围,它们也在本发明的范围内。
本技术领域的专业人员将会认识到,在被研究的群体中存在标记物的两个等位基因(例如SNPs),其中一个等位基因被发现在群体中具有性状或疾病的个体组中的频率高于对照组,而标记物的另一个等位基因被发现在具有性状或疾病的个体组中的频率低于对照组。在这种情况下,标记物的一个等位基因(被发现在具有性状或疾病的个体中频率增加的等位基因)将是有风险等位基因,而另一个等位基因将是保护性等位基因。
连锁不平衡
重组这种自然现象,对于每对染色体来说在每次减数分裂事件过程中平均发生一次,代表了自然界为序列(因此也为生物学功能)提供变异的一种方式。已经发现,基因组中的重组不是随机发生的;相反,在重组率的频率上存在很大的变化,产生了具有高重组频率的小区域(也被称为重组热点)和具有低重组频率的较大的区域,这通常被称为连锁不平衡(LD)区块(Myers,S.等,Biochem Soc Trans34:526-530(2006);Jeffreys,A.J.,等,Nature Genet 29:217-222(2001);May,C.A.等,Nature Genet 31:272-275(2002))。
连锁不平衡(LD)是指两个遗传元件的非随机的搭配。例如,如果特定遗传元件(例如多态性标记物的等位基因,或单倍型)在群体中出现的频率为0.50(50%),另一个元件出现的频率为0.50(50%),假设元件随机分配,那么一个个体具有两个元件的预计的发生率为0.25(25%)。但是,如果发现两个元件一起出现的频率高于0.25,那么元件被说成是连锁不平衡的,因为它们倾向于以比根据它们独立的出现频率(例如等位基因或单倍型频率)预测的比例更高的比例一起遗传。粗略地说,LD一般与两个元件之间的重组事件的频率相关。群体中等位基因或单倍型的频率可以通过对群体中的个体进行基因分型并确定群体中每个等位基因或单倍型出现的频率来测定。对于二倍体群体例如人类群体来说,个体一般对每个遗传元件(例如标记物、单倍型或基因)具有两个等位基因。
为了评估连锁不平衡(LD)的强度已经提出了许多不同的测量方法。大多数捕获成对的双等位基因位点之间关联的强度。LD的两个重要的成对度量是r2(有时称为Δ2)和|D’|。两种度量的范围都是从0(没有不平衡)到1(“完全”不平衡),但是它们的解释稍微有些不同。|D’|被定义为如果仅存在两个或三个可能的单倍型则等于1,如果所有四个可能的单倍型都存在则小于1。因此,小于1的|D’|值表明在两个位点之间可能在历史上已经发生过重组(回复突变也能导致|D’|小于1,但是对于单核苷酸多态性(SNPs)来说,通常认为这种情况发生的可能性比重组低)。度量r2表示两个位点之间的统计学相关性,如果只出现两个单倍型则取值为1。
r2度量大概是相关性作图的最具相关性的度量,因为在r2和检测易感性位点与SNPs之间的关联性所需的样品大小之间存在简单的反比关系。这些度量是针对成对位点定义的,但是对某些应用来说,可能需要确定在含有许多多态性位点的整个区域上LD有多强(例如测试在位点之间或群体范围内LD的强度是否有显著的差别,或者区域中的LD比在特定模型下预测的更高还是更低)。对整个区域的LD的测量不是直接进行的,而是使用一种方法测量r,这种方法是在群体遗传学中开发的。简单来说,r测量了在特定的群体模型下为了产生在数据中观察到的LD将需要多少重组。这种类型的方法也可能可以为确定LD数据是否为重组热点的存在提供了证据的难题提供统计学上严格的方法。对于本文描述的方法、试剂盒、步骤、介质和装置来说,显著的r2值可以是至少0.05,例如至少0.1、0.15、0.2、0.25、0.3、0.35、0.4、0.45、0.5、0.55、0.6、0.65、0.7、0.75、0.8、0.85、0.9、0.91、0.92、0.93、0.94、0.95、0.96、0.97、0.98、0.99或1.0。在一个优选实施方案中,显著的r2值可以是至少0.2。或者,本文描述的连锁不平衡是指其特征为|D’|的值为至少0.2,例如0.3、0.4、0.5、0.6、0.7、0.8、0.85、0.9、0.95、0.96、0.97、0.98、0.99的连锁不平衡。因此,连锁不平衡表示了不同标记物的等位基因之间的相关性。它由相关性系数或|D’|来度量(r2最高为1.0,|D’|最高为1.0)。在某些实施方案中,连锁不平衡根据r2和|D’|二者的测量值来定义。在一个这样的实施方案中,显著的连锁不平衡被定义为r2>0.1和|D’|>0.8。在另一个实施方案中,显著的连锁不平衡被定义为r2>0.2和|D’|>0.9。用于确定连锁不平衡的r2和|D’|值的其它组合和排列也是可能的,并在本发明的范围内。连锁不平衡可以如本文描述在单一人类群体中测定,或者可以在含有来自一个以上人类群体的个体的样品集合中测定。在本发明的一个实施方案中,LD在来自一个或多个HapMap群体(高加索人,非洲人,日本人,中国人)的样品中,按照(http://www.hapmap.org)的定义来测定。在一个这样的实施方案中,LD在HapMap样品的CEU群体中测定。在另一个实施方案中,LD在YRI群体中测定。在另一个实施方案中,LD在来自冰岛人群体的样品中测定。
如果在群体水平上基因组中的所有多态性是一致的,那么它们中每个单独的多态性将需要在关联性研究中进行研究。但是,由于多态性之间的连锁不平衡,紧密连锁的多态性是强烈相关的,这减少了在关联性研究中为了观察到显著相关性所需调查的多态性的数量。LD的另一个结果是,许多多态性由于它们是强烈相关的,可能给出关联性信号。
已经在基因组范围内产生了基因组LD谱图,这样的LD谱图已经被建议用作框架对疾病基因进行作图(Risch,N. & Merkiangas,K,Science 273:1516-1517(1996);Maniatis,N.等,Proc Natl Acad Sci USA99:2228-2233(2002);Reich,DE等,Nature 411:199-204(2001))。
现在已经确定,人类基因组的许多部分可以被分成一系列不连续的含有几个共同的单倍型的单倍型区块;对于这些区块来说,连锁不平衡数据几乎没有提供表明重组的证据(参见例如Wall.,J.D.和Pritchard,J.K.,Nature Reviews Genetics 4:587-597(2003);Daly,M.等,Nature Genet.29:229-232(2001);Gabriel,S.B.等,Science 296:2225-2229(2002);Patil,N.等,Science 294:1719-1723(2001);Dawson,E.等,Nature418:544-548(2002);Phillips,M.S.等,Nature Genet.33:382-387(2003))。
有两种主要的方法用于定义这些单倍型区块:区块可以被定义为具有有限的单倍型多样性的DNA区域(参见例如Daly,M.等,NatureGenet.29:229-232(2001);Patil,N.等,Science 294:1719-1723(2001);Dawson,E.等,Nature 418:544-548(2002);Zhang,K.等,Proc.Natl.Acad.Sci.USA 99:7335-7339(2002)),或者定义为使用连锁不平衡鉴定的具有广泛的历史上重组的过渡区之间的区域(参见例如Gabriel,S.B.等,Science 296:2225-2229(2002);Phillips,M.S.等,Nature Genet.33:382-387(2003);Wang,N.等,Am.J. Hum.Genet.71:1227-1234(2002);Stumpf,M.P.,和Goldstein,D.B.,Curr.Biol.13:1-8(2003))。更近些时候,在人类基因组范围上精细规模的重组率和相应的热点的图谱已经被产生(Myers,S.等,Science 310:321-32324(2005);Myers,S.等,Biochem Soc Trans 34:526530(2006))。图谱显示出在基因组范围内的重组中存在大量变化,在热点处重组率高达10-60cM/Mb,而在中间区域中接近于0,因此代表了有限单倍型多样性和高LD的区域。因此,谱图可用于将单倍型区块/LD区块定义为重组热点侧翼的区域。在本文中使用的术语“单倍型区块”或“LD区块”包含由任何上述的特征、或本技术领域的专业人员用于定义这种区域所用的其它可选方法定义的区块。
使用单个标记物或含有多个标记物的单倍型,单倍型区块可用于对表型和单倍型状态之间的相关性进行作图。可以在每个单倍型区块中鉴定主要的单倍型,然后可以鉴定一组“标签”SNPs或标记物(在单倍型中进行辨别所需的最小组的SNPs)。这些标签SNPs或标记物然后可用于评估来自个体组的样品,以便鉴定表型和单倍型之间的相关性。如果需要,可以对邻近的单倍型区块同时进行评估,因为在单倍型区块之间也可能存在连锁不平衡。
因此,显然,对于任何给定的观察到的与基因组中的多态性标记物的关联性来说,可能基因组中的其它标记物也显示出关联性。这是基因组范围内LD的不均衡分布的自然结果,正如通过重组率的大的变化所观察到的。因此,在某种意义上,用于检测关联性的标记物代表了与给定疾病或性状有关的基因组区域(即单倍型区块或LD区块)的“标签”,并因此可用于本发明的方法和试剂盒中。在被发现与疾病或性状有关的区域中可以存在有一个或多个引起结果的(功能性)变体或突变。这样的变体可赋予比用于检测关联性的标签标记物观察到的更高的相对风险(RR)或比值比(OR)。因此,如本文所述,本发明涉及了用于检测与疾病的关联性的标记物,以及与标记物连锁不平衡的标记物。因此,在本发明的某些实施方案中,与本文所述的本发明标记物和/或单倍型连锁不平衡的标记物可以被用作替代标记物。在一个实施方案中,替代标记物具有比最初被发现与本文所述的疾病相关的标记物或单倍型更小的相对风险(RR)和/或比值比(OR)值。在其它实施方案中,替代标记物具有比最初被发现与本文所述的疾病相关的标记物所最初测定的更大的RR或OR值。这样的实施方案的一个例子是与最初被发现与疾病相关的更常见的变体(>10%群体频率)、例如本文描述的变体连锁不平衡的稀有的或相对稀有的(<10%等位基因群体频率)变体。鉴定和使用这些标记物用于检测本文描述的本发明人发现的关联性,可以通过本技术领域的专业人员熟知的常规方法来进行,因此也在本发明的范围内。
与本文显示的与2型糖尿病有关联的标记物连锁不平衡的某些多态性标记物有可能位于定义的LD区块的物理边界之外。这是所述区域中历史上的重组率的结果,历史上的重组可能导致产生强烈连锁不平衡的区域(LD区块),它带有与区块内的标记物连锁不平衡的区块外的剩余标记物。这样的标记物也在本发明的范围内,因为由于它们与在本文中显示出的与2型糖尿病相关联的标记物的遗传关系,它们同样可用于实施本发明。例子显示在表22(rs17234378;SEQ ID NO:44)、表23(rs7086285;SEQ ID NO:43)和表24(rs9890889;SEQ ID NO:31;rs2009802;SEQ ID NO:38;rs17718938;SEQ ID NO:39;rs2109050;SEQ ID NO:41;rs1962801;SEQ ID NO:42)中。
单倍型频率的测定
患者和对照组中单倍型的频率可以使用期望最大化算法来估算(Dempster A.等,J.R.Stat.Soc.B,39:1-38(1977))。可以使用这种算法的推广形式来操作丢失的基因型和阶段的不确定性。在虚假设下,患者和对照被假定具有同样的频率。使用或然性方法,测试可选的假说,其中候选的有风险单倍型、可以包含本文描述的标记物、被允许在患者中比对照中具有更高的频率,而两个组中其它单倍型的频率的比率被假设是相同的。在两种假说下分别将或然性最大化,并使用相应的1-df或然性比率统计数值来评估统计学显著性。
为了在所需区域中寻找有风险和保护性的标记物和单倍型,研究了例如被基因分型的标记物的所有可能组合的关联性,只要那些标记物分布在可实践的区域中。可以将合并的患者和对照组随机分成两组,其大小与原始的患者和对照组相等。然后重复标记物和单倍型分析,确定记录到的最显著的p-值。这种随机化的流程可以被重复例如超过100次,以构建p-值的经验性分布。在优选实施方案中,p-值<0.05是显著的标记物和/或单倍型关联性的指示。
单倍型分析
进行单倍型分析的一种通用方法包括将基于或然性的推论用于NEsted MOdels(Gretarsdottir S.等,Nat.Genet.35:131-38(2003))。该方法在NEMO程序中执行,允许使用许多多态性标记物、SNPs和微卫星。方法和软件被特别设计用于病例-对照研究,其目的是鉴定赋予了不同风险的单倍型组。它也是用于研究LD结构的工具。在NEMO中,在EM算法的帮助下,直接计算了最大或然性估算值、或然性比率和p-值,对于观察到的数据,将其作为缺失数据问题进行处理。
尽管基于从观察到的数据直接计算的或然率的或然率比率检验捕捉到了由于阶段的不确定性和缺失的基因型而丢失的信息,可用于可靠地给出有效的p-值,但了解由于信息的不完整而丢失了多少信息,仍然是有意义的。用于单倍型分析的信息计算描述在Nicolae和Kong(技术报告537号,芝加哥大学统计学院统计学系;Biometrics,60(2):368-75(2004))中,作为为连锁分析定义的信息计算的自然延伸,并在NEMO中执行。
对于与疾病或性状(例如2型糖尿病)相关的单个标记物来说,可以使用Fisher精确检验来计算每个单独等位基因的双侧p-值。通常来说,除非特别指明,所有显示的p-值没有对多重比较进行调整。显示的频率(对于微卫星、SNPs和单倍型来说)是与携带者频率相反的等位基因频率。为了最小化由于作为连锁分析的家庭召集的患者的亲缘性造成的任何偏差,一级和二级亲属可以从患者名单中消除。此外,通过扩展在Risch,N.& Teng,J.(Genome Res.,8:1273-1288(1998))中描述的偏差调整步骤,合并具有亲缘关系的DNA(同上)以便它可以适用于普通家族关系、并呈递调整过的和未调整的p-值进行比较,可以重复检验,对患者间任何残留的亲缘性进行关联性校正。差异一般来说正如预期一样非常小。为了评估通过多次检验校正的单个标记物关联性的显著性,我们可以使用同样的基因型数据执行随机测试。可以将患者和对照组随机化,将关联性分析重做多次(例如多达500,000次),在某些重复中对某些标记物等位基因产生的p-值小于或等于我们使用最初的患者和对照组时观察到的p-值,这样的重复的分数即是p-值。
对于单个标记物和单倍型分析来说,通过假设的乘法模型(单倍型相对风险模型)可以计算相对风险(RR)和群体归因风险度(PAR)(Terwilliger,J.D.& Ott,J.,Hum.Hered.42:337-46(1992)和Falk,C.T.&Rubinstein,P,Ann.Hum.Genet.51(Pt 3):227-33(1987)),即一个人携带的两个等位基因/单倍型的风险的乘积。例如,如果A相对于a的风险是RR,那么人纯合子AA的风险将是杂合子Aa的风险的RR倍,以及纯合子aa的风险的RR2倍。乘法模型具有简化分析和计算的良好特性——单倍型在受影响的群体中以及对照群体中是独立的,即处于Hardy-Weinberg平衡。因此,受影响的和对照的单倍型的数量每个都具有多项式分布,但是在可选假设下具有不同的单倍型频率。具体来说,对于两个单倍型hi和hj来说,风险(hi)/风险(hj)=(fi/pi)(fj/pj),其中f和p分别是指受影响的群体和对照群体中的频率。尽管如果真正的模型不是乘法的将会损失一些效能,但除了极端情况下之外,损失趋于轻微。最重要的是,p-值总是有效的,因为它们是针对虚假设计算的。
风险评估和诊断学
正如本文所述,某些多态性标记物和含有这样的标记物的单倍型被发现可用于2型糖尿病的风险评估。风险评估可以包括使用标记物来诊断对2型糖尿病的易感性。多态性标记物的特定等位基因,在患有2型糖尿病的个体中发现的频率,比在没有诊断出2型糖尿病的个体中的频率更高。因此,这些标记物等位基因对于在个体中检测2型糖尿病或对2型糖尿病的易感性,具有预测价值。含有风险标记物、例如本文描述的标记物的单倍型区块或LD区块中的标签标记物,可用作单倍型区块或LD区块中其它标记物和/或单倍型的替代物。具有等于1的r2值的标记物是风险变体的完美的替代物,即一个标记物的基因型完美地预测了另一个的基因型。具有小于1的较小的r2值的标记物也可以作为风险变体的替代物,或者可选地代表相对风险值等于或可能甚至高于风险变体的变体。
鉴定到的风险变体本身可以不是功能变体本身,但是在这种情况下与真正的功能变体连锁不平衡。本发明包括为本文公开的标记物评估这样的替代标记物。这样的标记物被注解、作图和列表在本领域技术人员熟知的公共数据库(例如dbSNP)中,或者,可以通过对使用本发明的标记物在一组个体中鉴定到的区域或区域的一部分进行测序,并在得到的序列组中鉴定多态性,来容易地鉴定。结果,本技术领域的专业人员可以容易地并且不需要过度的实验,对与本文描述的标记物和/或单倍型连锁不平衡的替代标记物进行基因分型。与检测到的风险变体连锁不平衡的标签或替代标记物,对于在个体中检测与2型糖尿病的关联性或对2型糖尿病的易感性,也具有预测价值。
本发明的标记物和单倍型,例如在表1-24中显示的标记物,可单独或组合地用于风险评估和诊断目的。标记物和单倍型也可以与其它赋予对2型糖尿病的增加的风险的标记物组合。即使是在单个标记物造成的风险增加相对温和、即在10-30%的级别的情况下,关联性也可以具有明显的暗示。因此,相对普通的变体可能对总的风险有显著的贡献(群体归因风险度高),或者标记物的组合可用于定义基于标记物的组合风险、对发生疾病有显著的组合风险的个体组。因此,本文描述的与2型糖尿病相关联的标记物,可以与被报告或发现与2型糖尿病相关联的其它多态性标记物或单倍型组合,以获得基于大量遗传标记物的疾病的总体风险。
在一个这样的实施方案中,本文描述的多态性标记物或单倍型与TCF7L2基因中的标记物有关的信息一起被评估。该基因中的变体的关联性已经被建立(Grant S.F.等,Nat Genet.38:320-3(2006)),并且在大量的群体中得到了重复(Florez,J.C,Curr Opin Clin Nutr MetabolCare 10:391-396(2007))。TCF7L2基因中的标记物rs7903146或其它与该标记物连锁不平衡的标记物(例如rs12255372),可用于确定由基因中的风险变体赋予的遗传风险(OR大约为1.44)。
最近,已经暗示了其它基因中的标记物作为风险因子与2型糖尿病的病因学有关,包括PPARG(rs1801282)、KCNJ11(rs5215)、TCF2(rs4430796)、WFS1(rs10010131)、CDKN2A-2B(rs1081161)、IGF2BP2(rs4402960)和FTO(rs805136)(Frayling,T.M.Nature ReviewsGenetics 8:657-662(2007))。这些标记物或与其连锁不平衡的标记物,也可用于将2型糖尿病的风险变体的存在或不存在的确定与本文报告的变体相组合,以获得2型糖尿病的总体风险评估的方法中。
因此,在本发明的一个实施方案中,多个变体(遗传标记物和/或生物标记物和/或单倍型)被用于总体风险评估。在一个实施方案中,这些变体选自本文公开的变体。其它实施方案包括使用本发明的变体与其它已知用于诊断对2型糖尿病的易感性的变体的组合。在这样的实施方案中,在个体中确定了多个标记物和/或单倍型的基因型状态,并将个体的状态与相关变体的群体频率、或在临床健康的对象例如年龄匹配和性别匹配的对象中变体的频率进行比较。然后可以使用本技术领域已知的方法、例如多变量分析或联合风险分析,来确定根据多个位点处的基因型状态所赋予的总的风险。然后可以将基于这样的分析进行的风险评估用于本文描述的本发明的方法和试剂盒中。
如上所述,人类基因组的单倍型区块结构具有这样的作用,即与最初与疾病或性状相关的变体连锁不平衡的大量变体(标记物和/或单倍型)可以用作替代标记物,用于评估与疾病和性状的关联性。这样的替代标记物的数量依赖于几种因素,例如区域中的历史重组率、区域中的突变频率(即区域中多态性位点或标记物的数量)、以及区域中连锁不平衡的程度(LD区块的大小)。这些标记物通常位于使用本文描述的方法或通过本技术领域的专业人员已知的其它方法确定的所述LD区块或单倍型区块的物理边界内。但是,有时会发现标记物和单倍型关联性延伸到定义的单倍型区块的物理边界之外。在这样的情况下,这些标记物和/或单倍型也可用作物理上位于定义的单倍型区块内的标记物和/或单倍型的替代标记物和/或单倍型。因此,与本发明的标记物和单倍型连锁不平衡(典型的特征为r2大于0.1,例如r2大于0.2,包括r2大于0.3,也包括r2大于0.4)的标记物和单倍型也在本发明的范围之内,即使它们在物理上位于所定义的单倍型区块的边界之外。这包括了本文描述的标记物(例如列于表22、23和24中的标记物),但是也可以包括与表22、23和24中列出的一种或多种标记物连锁不平衡(例如特征为r2大于0.2,和/或|D’|>0.8)的其它标记物。
对于本文描述的SNP标记物来说,与在患者中被发现是过量的等位基因(风险等位基因)相反的等位基因,在2型糖尿病中被发现具有降低的频率。这些标记物和单倍型与这样的标记物连锁不平衡和/或含有它们,因此对2型糖尿病具有保护性,即它们赋予了携带有这些标记物和/或单倍型的个体以降低的发生2型糖尿病的风险或易感性。换句话说,对于双等位基因标记物例如SNPs来说,风险变体的风险等位基因的不存在,表明了另一种等位基因的存在。因此,本文描述的风险变体的不存在,表明了针对2型糖尿病的保护。
本文描述的含有遗传标记物例如SNPs和微卫星的组合的单倍型,可用于风险评估。单倍型的检测可以通过本技术领域已知的和/或本文描述的用于检测多态性位点的序列的方法来进行。此外,某些单倍型或标记物组与疾病表型之间的关联性可以使用标准的技术来证实。用于关联性的简单检验的一个代表性例子是在2x2表格上进行的Fisher精确检验。
在特定实施方案中,被发现与2型糖尿病有关的标记物或单倍型,是其中标记物或单倍型在具有2型糖尿病风险的个体(例如,受影响的人)中出现的频率,比它在健康个体(对照)中或在从群体中随机选择的个体(群体对照)中出现的频率高的标记物或单倍型,其中标记物等位基因或单倍型的存在是2型糖尿病或对2型糖尿病易感性的指示。在其它实施方案中,与一个或多个被发现与2型糖尿病有关的标记物连锁不平衡的风险标记物,是在具有2型糖尿病风险的个体(例如,受影响的人)中出现的频率比它们在对照中出现的频率高的标签标记物,其中标签标记物的存在是对2型糖尿病的增加的易感性的指示。在另一个实施方案中,与一个或多个被发现与2型糖尿病有关的标记物连锁不平衡的风险标记物等位基因(即赋予增加的易感性),是含有一个或多个在具有2型糖尿病风险的个体中出现的频率比它们在对照中出现的频率高的等位基因的标记物,其中标记物的存在是对2型糖尿病增加的易感性的指示。
研究群体
从普遍意义上讲,本发明的方法和试剂盒可以使用含有来自任何来源、即任何个体的基因组DNA的样品。在优选实施方案中,个体是人类个体。个体可以是成年人、儿童或胎儿。本发明还提供了对属于靶群体的成员的个体中标记物和/或单倍型的评估。在一个实施方案中,这样的靶群体是根据其它遗传因子、生物标记物、生物物理参数(例如体重、BMD、血压)、或总体健康和/或生活方式参数(例如疾病或相关疾病史、以前对疾病的诊断、疾病的家族史),具有发生疾病的风险的个体的群体或组。
本发明提供了包含特定年龄亚组的个体的实施方案,例如超过40岁、超过45岁、或超过50、55、60、65、70、75、80或85岁的年龄亚组。本发明的其它实施方案对应于其它的年龄组,例如年龄小于85岁的个体,例如小于80岁、小于75岁、或小于70、65、60、55、50、45、40、35或30岁。其它实施方案涉及发病时的年龄在任何上述年龄范围内的个体。在某些实施方案中,还考虑到了年龄的范围可以是相关的,例如发病时的年龄大于45岁但小于60岁。但是,其它的年龄范围也被考虑到了,包括由上面列出的年龄值界定的所有范围。本发明还涉及了任何性别、男性或女性的个体。
冰岛人群体是具有北欧血统的高加索人群体。在过去几年中,已经发表了大量的研究,报道了冰岛人群体中遗传连锁和关联性的结果。许多这些研究显示,最初在冰岛人群体中鉴定到的与特定疾病有关联的变体,在其它群体中得到了重复(Stacey,S.N.等,Nat Genet.May 272007(出版前的电子版);Helgadottir,A.等,Science 316:1491-93(2007);Steinthorsdottir,V.等,Nat Genet.39:770-75(2007);Gudmundsson,J.等,Nat Genet.39:631-37(2007);Amundadottir,L.T.等,Nat Genet.38:652-58(2006);Grant,S.F.等,Nat Genet.38:320-23(2006))。因此,在冰岛人群体中的遗传发现已经被普遍复制到其它群体中,包括来自非洲和亚洲的群体。本文描述的与CDKAL基因相关联的变体、特别是LD区块C06(SEQ ID NO:1),已经在欧洲、美洲和中国(香港)来源的几个群体中得到了重复。这支持了这样一种理念,即这些变体(rs7756992和与其连锁不平衡的标记物)在大多数人群中是2型糖尿病的风险变体。
因此,也考虑到了包含了个体人类群体的具体实施方案,并包含在本发明的范围内。这样的实施方案涉及来自一个或多个人类群体的人类对象,包括但不限于高加索人群体、欧洲人群体、美洲人群体、欧亚人群体、亚洲人群体、中/南亚人群体、东亚人群体、中东人群体、非洲人群体、西班牙人群体和大洋洲人群体。欧洲人群体包括但不限于瑞典人、挪威人、芬兰人、俄罗斯人、丹麦人、冰岛人、爱尔兰人、凯尔特人、英国人、苏格兰人、荷兰人、比利时人、法国人、德国人、西班牙人、葡萄牙人、意大利人、波兰人、保加利亚人、斯拉夫人、塞尔维亚人、波斯尼亚人、捷克人、希腊人和土耳其人群体。在其它实施方案中,本发明还可以在特定的人类群体中使用,包括班图人、Mandenk、约鲁巴人、San、木布提矮人、奥克尼群岛人、Adygel、俄罗斯人、萨丁尼亚人、托斯卡尼人、莫扎比特人、贝多因人、Druze、巴勒斯坦人、俾路支人、布拉灰人、莫克兰人、信德人、帕坦人、布鲁肖人、哈扎拉人、维吾尔人、Kalash、汉族人、傣族人、达斡尔人、赫哲族人、拉祜族人、苗族人、鄂伦春族人、畲族人、土家族人、土族人、锡伯族人、彝族人、内蒙古蒙古族人、纳西族人、柬埔寨人、日本人、雅库特人、美拉尼西亚人、巴布亚人、加里提亚拿人、Surui、哥伦比亚人、玛雅人和皮玛族人。
在一个优选实施方案中,本发明涉及含有非洲黑人血统的群体,例如含有非洲人血统或谱系的人的群体。非洲黑人血统可以通过自己报告确定为非洲裔美国人(African-Americans)、非裔美国人(Afro-Americans)、美国黑人(Black Americans),是黑色人种的一个成员,或尼格罗(negro)人种的一个成员。例如,非洲裔美国人或美国黑人是居住在北美并且起源于任何非洲黑色人种组的人。在另一个例子中,自己报告的非洲黑人血统的人可以具有至少一个非洲黑人血统的父母,或至少一个非洲黑人血统的祖父母。
个体对象中的种族来源也可以通过遗传分析来确定。血统的遗传分析可以使用不连锁的微卫星标记物来进行,例如在Smith等(Am JHum Genet 74,1001-13(2004))中提出的那些。
在某些实施方案中,本发明涉及了如上所述在特定群体中鉴定到的标记物和/或单倍型。本技术领域的专业人员将会认识到,在对不同的群体使用时,连锁不平衡(LD)测量将给出不同的结果。这是由于不同人类群体的不同群体史,以及在特定基因组区域中导致LD差异的不同选择压力。对于本技术领域的专业人员来说,某些标记物例如SNP标记物在不同群体中具有不同的群体频率,或者在一个群体中是多态性的但在另一个群体中不是,这种情况是熟知的。但是,本技术领域的专业人员将使用可用的或如本文设想的方法,在任何给定的人类群体中实施本发明。这可以包括评估本发明的LD区域中的多态性标记物,以便鉴定出那些在特定群体中表现出最强关联性的标记物。因此,本发明的有风险变体可以在不同的单倍型背景上、并以不同的频率存在于各种不同人类群体中。但是,使用本技术领域已知的方法和本发明的标记物,可以在任何给定人类群体中实施本发明。
遗传测试的用途
关于赋予了发生2型糖尿病的风险的遗传变体的知识,为使用遗传测试辨别具有发生疾病的增加的风险的个体(即风险变体的携带者)和具有发生疾病的降低的风险的个体(即保护性变体的携带者)提供了机会。对于属于上述两个组的个体来说,遗传测试的核心价值是能够在早期阶段诊断癌症的可能性,以及为临床医师提供关于疾病的预后/攻击性的信息,以便能够实施最合适的治疗。
例如,对2型糖尿病使用遗传测试,可以在具有受损的空腹葡萄糖(IFG)或受损的葡萄糖耐受性(IGT)的人中鉴定高风险个体。已经了解,尽管大约三分之一被发现具有IFG/IGT的人发生了2型糖尿病,但有同样比例的个体的葡萄糖水平回到了正常。在该组中鉴定是遗传风险变体的携带者的个体,将允许通过保护性措施对那些个体进行定向。例如,这些个体可以得益于更密切地监测血糖水平以帮助早期诊断。他们也可能需要更严紧的生活方式干预忠告,因为具有某些遗传风险因子的个体与没有这些因子的个体相比,在较低的BMI水平下发生2型糖尿病。
具有2型糖尿病家族史的个体和风险变体的携带者可以从遗传测试中获益,因为对遗传风险因子的存在的了解、或成为一个或多个风险因子的携带者的增加的风险的证据,可以为实行更健康的生活方式提供增加的激励。此外,对于这样的个体应该建议对葡萄糖水平进行更密切的监测,以促进早期诊断和/或预防性治疗。
2型糖尿病患者的遗传测试还可以给出关于疾病的首要原因的有价值的信息,并帮助临床医生对每个个体选择最适合的治疗选择和药物。例如,具有降低的胰岛素分泌的遗传风险因子的患者,可能可以得益于增加胰岛素分泌的药物,而在那些个体中增加胰岛素的敏感性可能不太有效。
发明方法
本文描述了对2型糖尿病进行风险评估的方法,这些方法包含在本发明中。本发明还包含了评估个体对2型糖尿病的治疗药剂发生反应的可能性的方法,以及用于预测治疗药剂对2型糖尿病的有效性的方法。本发明还包含了用于分析来自对象的样品、以检测对2型糖尿病的易感性的试剂盒。
本发明的诊断和筛选分析方法
在某些实施方案中,本发明涉及通过检测在2型糖尿病对象或对2型糖尿病易感的对象中出现得更频繁的遗传标记物的特定等位基因,来评估风险或诊断、或帮助评估风险或诊断2型糖尿病或对2型糖尿病易感性的方法。在特定实施方案中,本发明是通过检测至少一个多态性标记物(例如本文描述的标记物)的至少一个等位基因,来评估对2型糖尿病的易感性的方法。通过本发明描述的方法,特定标记物的特定等位基因或单倍型的检测,是对2型糖尿病易感性的指示。这样的预后或预测分析也可用于在2型糖尿病的症状发作之前,确定对象的预防性治疗。
在某些实施方案中,本发明涉及诊断、例如由专业医务人员执行的诊断的临床应用的方法,该方法可以包括评估或确定遗传风险变体。在其它实施方案中,本发明涉及由外行执行的风险评估(或诊断)的方法。在基因分型技术中的最新技术进展,包括SNP标记物的高通量基因分型、例如分子倒置探针阵列技术(例如Affymetrix GeneChip)和BeadArray技术(例如Illumina GoldenGate和Infinium分析),已经使个体对他们自己的基因组的多达一百万个SNPs进行评估成为可能。得到的对个体可用的基因型信息,可以与来自公开文献的关于与各种SNPs有关的疾病或性状风险的信息相比较。因此,本文描述的与疾病相关的等位基因的诊断应用,可以由健康专业人员根据临床测试的结果来进行,也可以由外行人来进行,包括为执行SNPs的全基因组评估提供服务的个体。换句话说,根据遗传风险对易感性进行的诊断或评估,可以由健康专业人员、遗传顾问、基因分型服务提供者或外行人,根据他/她的基因型的信息以及关于各种不同风险因子的出版物,来做出。在本发明的上下文中,术语“诊断”和“诊断易感性”,意指任何可用的诊断方法,包括上面提到的诊断方法。
此外,在某些其它的实施方案中,本发明涉及了通过检测在2型糖尿病患者中比在没有诊断出2型糖尿病的个体中或总人群中出现频率低的特定遗传标记物等位基因或单倍型,来诊断或帮助诊断对2型糖尿病的降低的易感性的方法。
正如本文所述和示例的,特定的标记物等位基因或单倍型(例如在表1-24中列出的标记物和单倍型,例如在表1-6和表9-12中列出的标记物和单倍型,以及与其连锁不平衡的标记物)与2型糖尿病有关。在一个实施方案中,标记物等位基因或单倍型是赋予了对2型糖尿病的显著风险或易感性的标记物等位基因或单倍型。在另一个实施方案中,本发明涉及在人类个体中诊断对2型糖尿病的易感性的方法,方法包括在从个体获得的核酸样品中确定至少一个多态性标记物的至少一个等位基因的存在或不存在,其中至少一个多态性标记物选自表9、表10、表11和表12中列出的多态性标记物,以及与其连锁不平衡(定义为r2>0.2)的标记物。在另一个实施方案中,本发明涉及通过筛选在表1-6和9-12中列出的至少一个标记物等位基因或单倍型、或与其连锁不平衡的标记物,在人类个体中诊断或评估2型糖尿病的易感性的方法。在另一个实施方案中,标记物等位基因或单倍型在患有2型糖尿病或对2型糖尿病易感的对象(受影响的)中出现的频率,与它在健康的对象(对照,例如群体对照)中出现的频率相比,更高。在某些实施方案中,至少一个标记物等位基因或单倍型的关联性的显著性的特征为p-值<0.05。在其它实施方案中,关联性的显著性的特征为p-值较小,例如<0.01、<0.001、<0.0001、<0.00001、<0.000001、<0.0000001、<0.00000001或<0.000000001。
在这些实施方案中,至少一个标记物等位基因或单倍型的存在是对2型糖尿病的易感性的指示。这些诊断方法包括检测与2型糖尿病有关的至少一个标记物等位基因或单倍型的存在或不存在。本文描述的单倍型包括各种不同遗传标记物(例如SNPs、微卫星)的组合。构成特定单倍型的特定遗传标记物等位基因的检测,可以通过本文描述的和/或本技术领域已知的各种不同方法来进行。例如,遗传标记物可以在核酸水平上(例如通过直接的核苷酸测序或通过其它本技术领域的专业人员已知的方法)检测,也可以在氨基酸水平上检测,如果遗传标记物影响由2型糖尿病相关核酸编码的蛋白的编码序列的话(例如,通过蛋白测序或通过使用识别这样的蛋白的抗体的免疫分析)。本发明的标记物等位基因或单倍型对应于与2型糖尿病有关的基因组DNA序列的片段。这样的片段含有所述的多态性标记物或单倍型的DNA序列,但是也可以含有与标记物或单倍型强烈LD(连锁不平衡)的DNA区段(例如由r2值大于0.2和/或|D’|>0.8所确定的)。
在一个实施方案中,对2型糖尿病的易感性的诊断或评估可以使用杂交方法来实现,例如Southern分析、Northern分析、和/或原位杂交(参见《分子生物学现代方法》(Current Protocols in MolecularBiology),Ausubel,F.等主编,John Wiley&Sons,包括所有附录)。特定标记物等位基因的存在可以通过特异性针对特定等位基因的核酸探针的序列特异性杂交来指示。一种以上特定标记物等位基因或特定单倍型的存在可以通过使用几种序列特异性核酸探针来指示,每种探针特异性针对特定等位基因。在一个实施方案中,单倍型可以通过特异性针对特定单倍型的(即与含有单倍型特征性的特定标记物等位基因的DNA链特异性杂交的)单一核酸探针来指示。序列特异性探针可以被导向以与基因组DNA、RNA或cDNA杂交。本文使用的“核酸探针”可以是与互补序列杂交的DNA探针或RNA探针。本技术领域的专业人员将了解如何设计这样的探针,使得只有在测试样品的基因组序列中存在特定等位基因时,才发生序列特异性杂交。
为了诊断对2型糖尿病的易感性,通过将含有2型糖尿病相关核酸的测试样品、例如基因组DNA样品,与至少一种核酸探针相接触来形成杂交样品。用于检测mRNA或基因组DNA的探针的非限制性例子是能够与本文描述的mRNA或基因组DNA序列杂交的标记的核酸探针。核酸探针可以是例如全长的核酸分子或其一部分,例如长度为至少15、30、50、100、250或500个核苷酸、在严紧条件下足以与适当的mRNA或基因组DNA特异性杂交的寡核苷酸。例如,核酸探针可以含有本文描述的LD区块C06(SEQ ID NO:1)、LD区块C10(SEQID NO:2)(例如编码IDE、KIF11和/或HHEX基因的核苷酸序列)、LD区块C17(SEQ ID NO:3),或CDKAL1基因或SLC30A8基因的核苷酸序列的全部或一部分,可选地含有本文描述的标记物的至少一个等位基因,或本文描述的至少一个单倍型,或者探针可以与这样的序列互补。在特定的实施方案中,核酸探针是本文描述的LD区块C06(SEQ ID NO:1)、LD区块C10(SEQ ID NO:2)(例如编码IDE、KIF11和/或HHEX基因的核苷酸序列)、LD区块C17(SEQ ID NO:3),或CDKAL1基因或SLC30A8基因的核苷酸序列的一部分,可选地含有本文描述的标记物的至少一个等位基因,或包含在本文描述的单倍型中的至少一个等位基因,或者探针可以是这样的序列的互补序列。其它适合用于本发明的诊断分析的探针在本文中进行了描述。杂交可以通过本技术领域的专业人员熟知的方法来进行(参见例如《分子生物学现代方法》(Current Protocols in Molecular Biology),Ausubel,F.等主编,John Wiley & Sons,包括所有附录)。在一个实施方案中,杂交是指特异性杂交,即没有错配的杂交(精确杂交)。在一个实施方案中,特异性杂交的杂交条件是高度严紧的。
特异性杂交、如果存在的话,使用标准方法来检测。如果在核酸探针与测试样品的核酸之间发生了特异性杂交,那么样品含有与核酸探针中存在的核苷酸互补的等位基因。对于本发明的任何标记物、或构成本发明的单倍型的标记物可以重复这个过程,或者可以同时使用多个探针在同一时间检测一个以上的标记物等位基因。设计含有一个以上特定单倍型的标记物等位基因的单个探针(例如探针含有与构成特定单倍型的2、3、4、5个或所有标记物互补的等位基因),也是可能的。样品中单倍型的特定标记物的检测表明样品源含有特定单倍型(例如单倍型),因此对疾病易感。
在一个优选实施方案中,使用的方法利用了在其3’末端含有荧光部分或基团、在5’末端含有淬灭剂的检测寡核苷酸探针和增强子寡核苷酸,如同Kutyavin等(Nucleic Acid Res.34:e128(2006))描述的。荧光部分可以是Gig Harbor Green或Yakima Yellow,或其它适合的荧光部分。检测探针被设计成与含有被检测的SNP多态性的短的核苷酸序列杂交。优选情况下,SNP位于从末端残基到距离检测探针的3’末端-6个残基之间的任何地方。增强子是在检测探针3’方向上与DNA模板杂交的短的寡核苷酸探针。探针被设计成当两个探针都与模板结合时,在检测探针和增强子核苷酸探针之间存在单个核苷酸缺口。缺口产生了合成的脱碱基位点,可以被内切核酸酶例如内切核酸酶IV识别。酶将染料从完全互补的检测探针上切下,但是不能切开含有错配的检测探针。因此,通过测量释放出的荧光部分的荧光,可以对由检测探针的核苷酸序列定义的特定等位基因的存在进行评估。
检测探针可以具有任何适合的大小,尽管优选情况下探针相对较短。在一个实施方案中,探针的长度为5-100个核苷酸。在另一个实施方案中,探针长度为10-50个核苷酸,在另一个实施方案中,探针长度为12-30个核苷酸。其它长度的探针也是可能的,并在本技术领域的专业人员的普通技能的范围内。在优选实施方案中,含有SNP多态性的DNA模板在检测前通过聚合酶链反应(PCR)进行扩增。在这样的实施方案中,被扩增的DNA被用作检测探针和增强子探针的模板。
在某些实施方案中,检测探针、增强子探针和/或用于通过PCR扩增模板的引物,包括使用修饰的碱基,包括修饰的A和修饰的G。使用修饰的碱基可用于调整核苷酸分子(探针和/或引物)与模板DNA的熔融温度,例如在含有低百分率G或C碱基的区域中增加熔融温度,其中可以使用具有与其互补的T形成三个氢键的能力的修饰的A,或者用于在含有高百分率G或C碱基的区域中降低熔融温度,例如使用在双链DNA分子中与其互补的C碱基只能形成两个氢键的修饰的G碱基。在优选实施方案中,修饰的碱基被用于设计检测核苷酸探针。在这些方法中可以选择任何专业技术人员已知的修饰碱基,根据本文的教导对适合的碱基进行选择也在专业人员的能力范围内,已知的碱基可以从专业人员已知的商业来源获得。
在另一种杂交方法中,Northern分析(参见《分子生物学现代方法》,Current Protocols in Molecular Biology,Ausubel,F.等主编,JohnWiley & Sons,同上)被用于鉴定与2型糖尿病相关的多态性的存在。对于Northern分析来说,RNA测试样品通过适合的方法从对象获得。如本文所示,核酸探针与来自对象的RNA的特异性杂交表明了特定等位基因与探针互补。关于核酸探针的使用的代表性的例子,参见例如美国专利Nos.5,288,611和4,851,330。
此外或可选地,在本文描述的杂交方法中可以在核酸探针以外或代替核酸探针而使用肽核酸(PNA)探针。PNA是DNA模拟物,具有类似肽的无机骨架例如N-(2-氨基乙基)甘氨酸单位,以及通过亚甲基羰基键连接到甘氨酸的氮上的有机碱基(A、G、C、T或U)(参见例如Nielsen,P.等,Bioconjug.Chem.5:3-7(1994))。PNA探针可以被设计成与怀疑含有一个或多个与2型糖尿病相关的标记物等位基因或单倍型的样品中的分子特异性杂交。因此,PNA探针的杂交可用于诊断2型糖尿病或对2型糖尿病的易感性。
在本发明的方法的一个实施方案中,2型糖尿病或对2型糖尿病的易感性的诊断,是通过来自对象的核酸的酶法扩增来实现的。例如,可以从对象获得含有基因组DNA的测试样品,并可以使用聚合酶链反应(PCR)扩增含有一个或多个本发明的被发现与2型糖尿病相关的标记物或单倍型的片段。如本文所述,与2型糖尿病相关的特定标记物等位基因或单倍型的鉴定,可以使用各种不同的方法来进行(例如序列分析、限制性消化分析、特异性杂交、单链构象多态性分析(SSCP)、电泳分析等)。在另一个实施方案中,诊断通过使用定量PCR(动力学热循环)进行表达分析来实现。该技术可以使用例如商业化的技术,例如(Applied Biosystems,Foster City,CA),来允许鉴定多态性和单倍型。该技术可以评估由2型糖尿病相关核酸编码的多肽或剪接变体的表达或组成中变化的存在。此外,变体的表达可以作为物理上或功能上的差异进行定量。
在本发明的方法的另一个实施方案中,通过限制性消化进行的分析可用于检测特定的等位基因,如果等位基因导致相对于参比序列来说产生或消除了限制性位点的话。从对象获得含有基因组DNA的测试样品。可以使用PCR从测试对象的测试样品中扩增与2型糖尿病相关的特定区域(例如表1-21的多态性标记物和单倍型,例如表1-6和表9-12的多态性标记物和单倍型,以及与其连锁不平衡的标记物)的核酸。可以进行限制性片段长度多态性(RFLP)分析,例如按照《分子生物学现代方法》(Current Protocols in Molecular Biology,同上)中的描述来进行。相关DNA片段的消化图谱表明了样品中特定等位基因的存在或不存在。
序列分析也可用于检测与2型糖尿病相关的多态性位点上的特定等位基因(例如表1-24的多态性标记物和单倍型,例如表1-6和表9-12的多态性标记物和单倍型,以及与其连锁不平衡的标记物,例如表22、23和24中显示的标记物)。因此,在一个实施方案中,确定特定标记物等位基因或单倍型的存在或不存在,包括了序列分析。例如,可以从测试对象获得DNA或RNA的测试样品。可以使用PCR或其它适合的方法扩增2型糖尿病相关核酸的一部分,然后可以通过对样品中基因组DNA的多态性位点(或多个多态性位点)进行测序来直接检测特定等位基因的存在。
通过使用扩增的寡核苷酸与等位基因特异性寡核苷酸(ASO)探针的斑点印迹杂交(参见例如Saiki,R.等,Nature,324:163-166(1986)),等位基因特异性寡核苷酸也可用于检测2型糖尿病相关核酸(例如表1-21的多态性标记物和单倍型,例如表1-6和表9-12的多态性标记物和单倍型,以及与其连锁不平衡的标记物)的特定等位基因的存在。“等位基因特异性寡核苷酸”(在本文中也称为“等位基因特异性寡核苷酸探针”)是大约10-50个碱基对或大约15-30个碱基对的寡核苷酸,与2型糖尿病相关核酸特异性杂交,并在多态性位点(例如本文描述的多态性)上含有特定等位基因。特异性针对一个或多个特定2型糖尿病相关核酸的等位基因特异性寡核苷酸探针,可以使用标准的方法来制备(参见例如《分子生物学现代方法》,Current Protocols inMolecular Biology,同上)。可以使用PCR来扩增2型糖尿病相关核酸的所需区域。含有扩增的区域的DNA可以使用标准方法进行斑点印迹(参见例如《分子生物学现代方法》,(Current Protocols in MolecularBiology),同上),并将印迹与寡核苷酸探针相接触。然后可以检测探针与扩增区域的特异性杂交的存在。等位基因特异性寡核苷酸探针与来自对象的DNA的特异性杂交,是与2型糖尿病相关的多态性位点上特定等位基因的指示(参见例如Gibbs,R.等,Nucleic Acids Res.,17:2437-2448(1989)和WO 93/22456)。
在另一个实施方案中,与来自对象的靶核酸序列区段互补的寡核苷酸探针的阵列,可用于在2型糖尿病相关核酸(例如表1-24的多态性标记物和单倍型,例如表1-6和表9-12的多态性标记物和单倍型,以及与其连锁不平衡的标记物)中鉴定多态性。例如,可以使用寡核苷酸阵列。寡核苷酸阵列典型地包含多个连接到基质表面上不同已知位置上的不同的寡核苷酸探针。这些寡核苷酸阵列、也被称为“基因芯片”(GenechipsTM),在本技术领域中已经广泛描述过了(参见例如美国专利No.5,143,854、PCT专利申请Nos.WO 90/15070和92/10092)。一般来说,这些阵列可以使用机械合成方法或组合了光蚀刻方法和固相寡核苷酸合成方法的光指导的合成方法来生产(Fodor,S.等,Science,251:767-773(1991);Pirrung等,美国专利No.5,143,854(也参见已出版的PCT申请No.WO 90/15070);和Fodor.S.等,已出版的PCT申请No.WO 92/10092和美国专利No.5,424,186,每个文件的全部内容在此引为参考)。使用机械合成方法合成这些阵列的技术描述在例如美国专利No.5,384,261中,其全部内容在此引为参考。在另一个实施方案中,可以使用线性阵列。
关于使用寡核苷酸阵列检测多态性其它描述可以在例如美国专利Nos.5,858,659和5,837,832中发现,这两个专利在此以其全文引为参考。可以使用其它的核酸分析方法来检测与2型糖尿病相关的多态性位点(例如表1-24的多态性标记物和单倍型,例如表1-6和表9-12的多态性标记物和单倍型,以及与其连锁不平衡的标记物)中的特定等位基因。代表性的方法包括例如直接手动测序(Church和Gilbert,Proc.Natl.Acad.Sci.USA,81:1991-1995(1988);Sanger,F.等,Proc.Natl.Acad.Sci.USA,74:5463-5467(1977);Beavis等,美国专利No.5,288,644);自动化荧光测序;单链构象多态性分析(SSCP);钳位(clamped)变性凝胶电泳(CDGE);变性梯度凝胶电泳(DGGE)(Sheffield,V.等,Proc.Natl.Acad.Sci.USA,86:232-236(1989));迁移率变动分析(Orita,M.等,Proc.Natl.Acad.Sci.USA,86:2766-2770(1989));限制性酶分析(Flavell,R.等,Cell,15:25-41(1978);Geever,R.等;Proc.Natl.Acad.Sci.USA,78:5081-5085(1981));异源双链分析;化学错配裂解(CMC)(Cotton,R.等,Proc.Natl.Acad.Sci.USA,85:4397-4401(1985));RNase保护分析(Myers,R.等,Science,230:1242-1246(1985));使用识别核苷酸错配的多肽例如大肠杆菌mutS蛋白;以及等位基因特异性PCR。
在本发明的另一个实施方案中,对2型糖尿病或对2型糖尿病的易感性的诊断,在本文描述的遗传标记物或单倍型导致了多肽的组成或表达发生改变的情况下,可以通过检查2型糖尿病相关的核酸编码的多肽的表达和/或组成来进行。因此,对2型糖尿病易感性的诊断,在本文描述的遗传标记物或单倍型导致了多肽的组成或表达发生改变的情况下,可以通过检测这些多肽中的一个、或2型糖尿病相关核酸编码的另一个多肽的表达和/或组成来进行。本发明的显示出与2型糖尿病的关联性的单倍型和标记物,可能通过它们对这些邻近基因中的一个或多个的影响来发挥作用。影响这些基因的可能机制包括例如影响转录、影响RNA剪接、改变mRNA可选剪接形式的相对量、影响RNA稳定性、影响从细胞核向细胞质的运输、以及影响翻译的效率和精确性。
各种不同的方法可用于进行这样的检测,包括酶联免疫吸附分析(ELISA)、Western印迹、免疫沉淀和免疫荧光。评估了来自对象的测试样品中由2型糖尿病相关核酸编码的多肽在表达中的变化和/或组成中的变化的存在。由2型糖尿病相关核酸编码的多肽在表达中的变化可以是例如定量的多肽表达(即产生的多肽的量)的变化。由2型糖尿病相关核酸编码的多肽的组成的变化是定性的多肽表达(例如突变体多肽的表达或不同剪接变体的表达)的变化。在一个实施方案中,对2型糖尿病易感性的诊断,通过检测由2型糖尿病相关核酸编码的特定剪接变体或剪接变体的特定图谱来进行。
也可以同时存在这两种变化(定量的和定性的)。本文中使用的多肽表达或组成中的“变化”,是指测试样品中多肽的表达或组成、与对照样品中2型糖尿病相关核酸编码的多肽的表达或组成相比的变化。对照样品是与测试样品相应的样品(即来自于同样类型的细胞),来自于没有感染2型糖尿病、和/或不具有对2型糖尿病易感性的对象(例如不具有本文描述的标记物等位基因或单倍型的对象)。类似地,与对照样品相比,测试样品中一个或多个不同剪接变体的存在,或测试样品中显著不同量的不同剪接变体的存在,可以表明对2型糖尿病的易感性。在等位基因相对于对照样品中的参比改变了剪接位点的情况下,与对照样品相比,测试样品中多肽的表达或组成的变化可以指示特定的等位基因。用于检测2型糖尿病相关核酸编码的多肽的表达和组成的各种不同的方法都可以使用,包括光谱法、比色法、电泳、等电聚焦和免疫分析(例如David等,美国专利No.4,376,110)例如免疫印迹(参见例如《分子生物学现代方法》,(Current Protocols inMolecular Biology),特别是第10章,同上)。
例如,在一个实施方案中,可以使用能够与2型糖尿病相关核酸编码的多肽结合的抗体(例如具有可检测标记的抗体)。抗体可以是多克隆的,也可以是单克隆的。可以使用完整的抗体,也可以使用其片段(例如Fv、Fab、Fab’、F(ab’)2)。对于探针或抗体来说,术语“标记的”意指包含了通过将可检测物质连接(即物理连接)到探针或抗体上以对探针或抗体直接标记,以及通过与被直接标记的另一种试剂进行反应而对探针或抗体的间接标记。间接标记的例子包括使用标记的第二抗体(例如荧光标记的第二抗体)检测第一抗体,以及用生物素对DNA探针进行末端标记,以便可以使用荧光标记的链亲和素来检测它。
在这种方法的一个实施方案中,将测试样品中2型糖尿病相关核酸编码的多肽的水平或量,与对照样品中2型糖尿病相关核酸编码的多肽的水平或量进行比较。测试样品中多肽的水平或量高于或低于对照样品中多肽的水平或量,使得差异在统计学上显著,表明了2型糖尿病相关核酸编码的多肽的表达发生了变化,并且诊断了负责引起表达变化的特定等位基因或单倍型。或者,将测试样品中2型糖尿病相关核酸编码的多肽的组成与对照样品中2型糖尿病相关核酸编码的多肽的组成进行比较。在另一个实施方案中,可以对测试样品和对照样品中的多肽的水平或量以及组成二者都进行评估。
在另一个实施方案中,对2型糖尿病的易感性的诊断,通过检测至少一个2型糖尿病相关标记物等位基因或单倍型(例如表1-21、例如表1-6和表9-12中列出的标记物的相关的等位基因或单倍型),以及与其它基于蛋白的、基于RNA的或基于DNA的分析方法相结合来进行。本发明的方法也可以与对象的家族史和风险因子(例如环境风险因子、生活方式风险因子)的分析组合起来使用。
试剂盒
用于本发明方法的试剂盒包含可用于本文描述的任何方法的组分,包括例如用于核酸扩增的引物,杂交探针,限制性酶(例如用于RFLP分析),等位基因特异性寡核苷酸,与本文描述的本发明的核酸(例如含有至少一个本发明的多态性标记物和/或单倍型的基因组区段)编码的改变的多肽、或本文描述的本发明的核酸编码的未改变的(天然)多肽结合的抗体,用于扩增与2型糖尿病有关的核酸的手段,用于分析与2型糖尿病有关的核酸的核酸序列的手段,用于分析与2型糖尿病有关的核酸编码的多肽(例如由2型糖尿病基因编码的2型糖尿病蛋白)的氨基酸序列的方法,等。试剂盒可以包含例如必需的缓冲液、用于扩增本发明的核酸(例如含有本文描述的一个或多个多态性标记物的核酸区段)的核酸引物、以及使用这些引物对扩增的片段进行等位基因特异性检测的试剂和必需的酶(例如DNA聚合酶)。此外,试剂盒可以提供与本发明的方法组合使用的分析方法的试剂,例如用于其它2型糖尿病的诊断分析方法的试剂。
在一个实施方案中,本发明是用于分析从对象获得的样品、以在对象中检测2型糖尿病、与2型糖尿病有关的症状、或对2型糖尿病的易感性的存在,其中试剂盒含有在个体的基因组中选择性检测本发明的至少一个多态性的至少一个等位基因所需的试剂。在特定的实施方案中,试剂含有能够与个体的基因组中含有至少一个本发明的多态性的片段杂交的至少一个连续的寡核苷酸。在另一个实施方案中,试剂含有至少一对能够与从对象获得的基因组区段的相反链杂交的寡核苷酸,其中每个寡核苷酸引物对被设计成选择性扩增个体的基因组中含有至少一个多态性的片段,其中多态性选自表1-6和9-12中列出的多态性,以及与其连锁不平衡的多态性标记物(例如在表22、23、和24中显示的标记物)。在另一个实施方案中,片段的大小为至少20个碱基对。这样的寡核苷酸或核酸(例如寡核苷酸引物)可以使用表明了2型糖尿病的多态性(例如SNPs或微卫星)两侧的核酸序列的部分来设计。在另一个实施方案中,试剂盒含有一个或多个标记的核酸,能够对与2型糖尿病有关的一个或多个特定多态性标记物或单倍型进行等位基因特异性检测,以及用于检测标记的试剂。适合的标记包括例如放射性同位素、荧光标记、酶标记、酶辅助因子标记、磁性标记、自旋标记、表位标记。
在特定实施方案中,通过试剂盒的试剂检测的多态性标记物或单倍型包含一个或一个以上标记物、两个或两个以上标记物、三个或三个以上标记物、四个或四个以上标记物或五个或五个以上标记物,标记物选自表9-12中显示的标记物。在另一个实施方案中,被检测的标记物或单倍型包含表22-24中显示的标记物。在另一个实施方案中,被检测的标记物或单倍型包括标记物rs2497304(SEQ ID NO:16)、rs947591(SEQ ID NO:30)、rs10882091(SEQ ID NO:4)、rs7914814(SEQ ID NO:24)、rs6583830(SEQ ID NO:20)、rs2421943(SEQ IDNO:15)、rs6583826(SEQ ID NO:19)、rs7752906(SEQ ID NO:32)、rs1569699(SEQ ID NO:6)、rs7756992(SEQ ID NO:21)、rs9350271(SEQ ID NO:33)、rs9356744(SEQ ID NO:34)、rs9368222(SEQ IDNO:35)、rs10440833(SEQ ID NO:36)、rs6931514(SEQ ID NO:37)、rs1860316(SEQ ID NO:10)、rs1981647(SEQ ID NO:11)、rs1843622(SEQ ID NO:9)、rs2191113(SEQ ID NO:13)和rs9890889(SEQ IDNO:31),以及与其连锁不平衡的标记物。在一个这样的实施方案中,连锁不平衡由r2的值大于0.2所定义。
在一个优选实施方案中,用于检测本发明的标记物的试剂盒含有与含有被检测的SNP多态性的模板DNA区段杂交的检测寡核苷酸探针,增强子寡核苷酸探针和内切核酸酶。正如前面解释的,检测寡核苷酸探针在其3’末端含有荧光部分或基团,在其5’末端含有淬灭剂,增强子寡核苷酸按照Kutyavin等(Nucleic Acid Res.34:e128(2006))的描述使用。荧光部分可以是Gig Harbor Green或Yakima Yellow,或其它适合的荧光部分。检测探针被设计成与含有被检测的SNP多态性的短核苷酸序列杂交。优选情况下,SNP位于从末端残基到离检测探针的3’末端-6个残基之间的任何位置。增强子是在检测探针3’方向上与DNA模板杂交的短的寡核苷酸探针。探针被设计成当两个探针都与模板结合时,在检测探针和增强子核苷酸探针之间存在单个核苷酸缺口。缺口产生了合成的脱碱基位点,可以被内切核酸酶例如内切核酸酶IV识别。酶将染料从完全互补的检测探针上切下,但是不能切开含有错配的检测探针。因此,通过测量释放出的荧光部分的荧光,可以对由检测探针的核苷酸序列定义的特定等位基因的存在进行评估。
检测探针可以具有任何适合的大小,尽管优选情况下探针相对较短。在一个实施方案中,探针的长度为5-100个核苷酸。在另一个实施方案中,探针长度为10-50个核苷酸,在另一个实施方案中,探针长度为12-30个核苷酸。其它长度的探针也是可能的,并在本技术领域的专业人员的普通技能的范围内。
在优选实施方案中,含有SNP多态性的DNA模板在检测前通过聚合酶链反应(PCR)进行扩增,用于这种扩增的引物被包含在试剂盒中。在这样的实施方案中,被扩增的DNA被用作检测探针和增强子探针的模板。
在检测探针、增强子探针和/或用于通过PCR扩增模板的引物的某些实施方案中,包括了使用修饰的碱基,包括修饰的A和修饰的G。使用修饰的碱基可用于调整核苷酸分子(探针和/或引物)与模板DNA的熔融温度,例如在含有低百分率G或C碱基的区域中增加熔融温度,其中可以使用具有与其互补的T形成三个氢键的能力的修饰的A,或者用于在含有高百分率G或C碱基的区域中降低熔融温度,例如使用在双链DNA分子中与其互补的C碱基只能形成两个氢键的修饰的G碱基。在优选实施方案中,修饰的碱基被用于设计检测核苷酸探针。在这些方法中,可以选择任何专业技术人员已知的修饰碱基,根据本文的教导对适合的碱基进行选择也在专业人员的能力范围内,已知的碱基可以从专业人员已知的商业来源获得。
在一个这样的实施方案中,标记物或单倍型的存在是对2型糖尿病的易感性(增加的易感性或降低的易感性)的指示。在另一个实施方案中,标记物或单倍型的存在是对2型糖尿病治疗剂的反应的指示。在另一个实施方案中,标记物或单倍型的存在是对2型糖尿病预后的指示。在另一个实施方案中,标记物或单倍型的存在是对2型糖尿病治疗的进展的指示。这样的治疗可以包括外科干预、医药或通过其它手段(例如生活方式改变)。
2型糖尿病的治疗剂
目前可用的2型糖尿病药物(除了胰岛素之外)分为6个主要的药物类别:磺酰脲类、格列奈类、双胍类、噻唑烷二酮类、α-葡萄糖苷酶抑制剂和一类被称为DPP-4抑制剂的新的药物。这些类别的药物以不同的方式发挥作用,以降低血糖水平。
1.磺酰脲类。磺酰脲类刺激胰腺β细胞释放更多的胰岛素。
2.格列奈类。格列奈类也是刺激β细胞释放胰岛素的药物。
3.双胍类。双胍类主要通过降低肝脏产生的葡萄糖的量来降低血糖水平。二甲双胍(美福明(Metformin))也通过使肌肉组织对胰岛素更敏感以便吸收葡萄糖来降低血糖水平。
4.噻唑烷二酮类。这些药物帮助胰岛素在肌肉和脂肪中的作用发挥得更好,也降低肝脏中的葡萄糖生产。
5.α-葡萄糖苷酶抑制剂。这些药物通过阻断淀粉、例如面包、土豆和通心面在肠道中的分解来降低血糖水平。它们也减缓某些糖类例如砂糖的分解。它们的作用减缓了用餐后血糖水平的升高。它们应该与第一口餐一起服用。
6:DPP-4抑制剂。一类被称为DPP-4抑制剂的新药物帮助增加A1C而不引起高血糖。它们通过阻止身体中天然存在的化合物GLP-1的分解而起作用。GLP-1降低身体中的血糖水平,但是被分解得非常快,使得当它作为药物本身被注射时不能很好地发挥作用。通过干扰GLP-1分解的过程,DPP-4抑制剂允许它在体内保持更长时间的活性,并只有当它们升高时才降低血糖水平。
这些类别中可用药物的例子列于药剂表1中。
药剂表1
药物类别 | 通用名 | 商标名 |
双胍类 | 二甲双胍 | Glucophage,Glucophage XR,Glycon |
二甲双胍加格列本脲 | Glucovance | |
噻唑烷二酮类 | 吡格列酮 | Actos |
罗格列酮 | Avandia | |
磺酰脲类 | 乙酰磺环己脲 | Dymelor |
氯磺丙脲 | Diabinese | |
格列其特达美康 | Diamicron MR | |
格列美脲 | Amaryl | |
格列吡嗪 | Glucotrol,Glucotrol XL | |
格列本脲 | Micronase,DiaBeta,Glynase Pres Tab | |
格列本脲加二甲双胍 | Glucovance | |
妥拉磺脲 | Tolinase | |
甲苯磺胺丁脲 | Orinase,Tol-Tab | |
格列奈类 | 那格列奈 | Starlix |
瑞格列奈 | Prandin,Gluconorm | |
α-葡萄糖苷酶抑制剂 | 阿卡波糖 | Precose,Prandase |
米格列醇 | Glyset | |
DPP-4抑制剂 | 西他列汀 | Januvia |
此外,包含双胍和磺酰脲的组合疗法已经被用于治疗2型糖尿病。
其它的2型糖尿病药物列于药剂表2中。
药剂表2
化合物名 | 化合物名(使用Autonom,ISIS Draw2.5版从MDL信息系统产生) | 公司 | 化合物参考文献 | 说明 |
AR-0133418(SN-4521) | 1-(4-甲氧基-苯甲基)-3-(5-硝基-噻唑-2-基)-脲 | AstraZeneca | AD | |
AR-025028 | NSD | AstraZeneca | ||
CT-98023 | N-[4-(2,4-二氯-苯基)-5-(1H-咪唑-2-基)-嘧啶-2-基]-N’-(5-硝基-吡啶-2-基)-乙烷-1,2-二胺 | Chiron Corp | 非胰岛素依赖性糖尿病 | |
CT-20026 | NSD | Chiron Corp | Wagman等,CurrPham.Des 2004:10(10)1105-37 | 非胰岛素依赖性糖尿病 |
CT-21022 | NSD | Chiron Corp | 非胰岛素依赖性糖尿病 | |
CT-20014 | NSD | Chiron Corp | 非胰岛素依赖性糖尿病 | |
CT-21018 | NSD | Chiron Corp | 非胰岛素依赖性糖尿病 | |
CHIR-98025 | NSD | Chiron Corp | 非胰岛素依赖性糖尿病 | |
CHIR-99021 | NSD | Chiron Corp | Wagman等,CurrPham.Des 2004:10(10)1105-37 | 非胰岛素依赖性糖尿病 |
CG-100179 | NSD | CrystalGenomics and Yuyu | WO-2004065370 | 糖尿病(韩国) |
4-[2-(4-二甲基氨基-3-硝基-苯基氨基)-嘧啶-4-基]-3,5-二甲基-1H-吡咯-2-腈 | Cyclacel Ltd. | 非胰岛素依赖性糖尿病以及其它 | ||
NP-01139,NP-031112,NP-03112,NP-00361 | 4-苯甲基-2-甲基-[1,2,4]噻唑烷-3,5-二酮 | NeuropharmaSA | CNS疾病,AD | |
3-[9-氟-2-(哌啶-1-羰基)-1,2,3,4-四氢-[1,4]diazepino[6,7,1-hi]吲哚-7-基]-4-咪唑[1,2-a]吡啶-3-基-吡咯-2,5-二酮 | Eli Lilly & Co | 非胰岛素依赖性糖尿病 | ||
GW-784752x,GW-784775,SB-216763,SB-415286 | 环戊烷羧酸(6-吡啶-3-基-furo[2,3-d]嘧啶-4-基)-酰胺 | GSK | WO-03024447(参比化合物:4-[2-(2-溴苯基)-4-(4-氟苯基)-1H-咪唑-5-基]吡啶) | 非胰岛素依赖性糖尿病,神经退行性疾病 |
NNC-57-0511,NNC-57-0545,NNC-57-0588 | 1-(4-氨基-呋咱-3-基)-5-哌啶-1-基甲基-1H-[1,2,3]噻唑-4-羧酸[1-吡啶-4-基-(E)亚甲基]-酰肼 | Novo Nordisk | 非胰岛素依赖性糖尿病 | |
CP-70949 | NSD | Pfizer | 降血糖药 | |
VX-608 | NSD | 脑血管局部缺血,非胰岛素依赖性糖尿病 |
KP-403类 | NSD | Kinetek | 核因子κB调节剂,抗炎性,细胞周期抑制剂,糖原合成酶激酶-3β抑制剂 | |
BYETTA(艾塞那肽) | 艾塞那肽:C184H282N50O60S,氨基酸序列为:H-His-Gly-Glu-Gly-Thr-Phe-Thr-Ser-Asp-Leu-Ser-Lys-Gln-Met-Glu-Glu-Glu-Ala-Val-Arg-Leu-Phe-He-Glu-Trp-Leu-Lys-Asn-Gly-Gly-Pro-Ser-Ser-Gly-Ala-Pro-Pro-Pro-Ser-NH2 | Amylin/EliLilly & Co | 非胰岛素依赖性糖尿病 | |
维达列汀(LAF237) | NSD | Novartis | 非胰岛素依赖性糖尿病,DPP-4抑制剂 |
本发明的治疗剂
本发明的变体(例如本文描述的标记物和/或单倍型)可用于鉴定新的2型糖尿病的治疗性靶。例如,含有与2型糖尿病相关的变体(标记物和/或单倍型)的基因或与其连锁不平衡的基因或它们的产物,以及被这些变体基因或它们的产物直接或间接调控或与它们相互作用的基因或它们的产物,可以成为开发治疗性药剂的靶,以治疗2型糖尿病、或阻止或延迟与2型糖尿病有关的症状的出现。治疗性药剂可以含有一种或多种例如小的非蛋白和非核酸分子、蛋白、肽、蛋白片段、核酸(DNA、RNA)、PNA(肽核酸),或它们的可以调节靶基因或它们的基因产物的功能和/或水平的衍生物或模拟物。
本发明的核酸和/或变体、或含有它们的互补序列的核酸,可用作反义构建物以控制细胞、组织或器官中的基因表达。与反义技术有关的方法对于专业技术人员来说是众所周知的,被描述和综述在《反义药物技术:原理,策略与应用》中(AntisenseDrug Technology:Principles,Strategies,and Applications,Crooke主编,Marcel Dekker Inc.,New York(2001))。一般来说,反义核酸分子被设计成与基因表达的mRNA的区域互补,使得反义分子与mRNA杂交,从而阻断mRNA翻译成蛋白。对于本技术领域的专业人员来说,已知有几类反义寡核苷酸,包括裂解物和阻断物。前者与靶RNA位点结合,激活细胞内核酸酶(例如RnaseH或Rnase L),并水解靶RNA。阻断物与靶RNA结合,通过对核糖体进行空间位阻来抑制蛋白的翻译。阻断物的例子包括核酸、吗啉代化合物、锁核酸和甲基膦酸酯(Thompson,Drug Discovery Today,7:912-917(2002))。反义寡核苷酸可直接用作治疗性药剂,也可用于确定和证实基因的功能,例如通过基因敲除或基因击落实验。反义技术被进一步描述在Lavery等,Curr.Opin.Drug Discov.Devel.6:561-569(2003),Stephens等,Curr.Opin.Mol.Ther.5:118-122(2003),Kurreck,Eur.J.Biochem.270:1628-44(2003),Dias等,Mol.Cancer Ter.1:347-55(2002),Chen,Methods Mol.Med.75:621-636(2003),Wang等,Curr.Cancer Drug Targets 1:177-96(2001)和Bennett,Antisense Nucleic AcidDrug.Dev.12:215-24(2002)中。
本文描述的变体可用于选择和设计特异性针对特定变体的反义试剂。使用关于本文描述的变体的信息,可以设计出特异性靶向含有本发明的一个或多个变体的mRNA分子的反义寡核苷酸或其它反义分子。通过这种方式,含有一个或多个本发明的变体(标记物和/或单倍型)的mRNA分子的表达可以被抑制或阻断。在一个实施方案中,反义分子被设计成与靶核酸的特定等位基因形式(即一个或几个变体(等位基因和/或单倍型))特异性结合,从而抑制源自于该特定等位基因或单倍型的产物的翻译,但是不与靶核酸分子在特定多态性位点上的其它或可选的变体结合。
由于反义分子可用于失活mRNA以便抑制基因表达,从而抑制蛋白表达,因此分子可用于治疗疾病或紊乱,例如2型糖尿病。该方法可以包括用含有与mRNA中的一个或多个区域互补的核苷酸序列的核酶进行裂解,以减弱mRNA被翻译的能力。这样的mRNA区域包括例如蛋白编码区,特别是对应于蛋白的催化活性、底物和/或配体结合位点或其它功能性结构域的蛋白编码区。
自从最初在线虫(C.elegans)中发现以来(Fire等,Nature391:806-11(1998)),在最近的十年中,对RNA干扰(RNAi)现象的研究非常活跃,在近年中,也在积极推行它在治疗人类疾病中的潜在应用(综述在Kim & Rossi,Nature Rev.Genet.8:173-204(2007)中)。RNA干扰(RNAi),也被称为基因沉默,其基础是使用双链RNA分子(dsRNA)关闭特定的基因。在细胞中,细胞质双链RNA分子(dsRNA)被细胞复合物加工成小干扰RNA(siRNA)。siRNA指导蛋白-RNA复合物靶向靶mRNA上的特定位点,导致mRNA的裂解(Thompson,DrugDiscovery Today,7:912-917(2002))。典型情况下,siRNA分子长度为大约20、21、22或23个核苷酸。因此,本发明的一个方面涉及分离的核酸分子,以及将那些分子用于RNA干扰,即作为小干扰RNA分子(siRNA)。在一个实施方案中,分离的核酸分子的长度为18-26个核苷酸,优选长度为19-25个核苷酸,更优选长度为20-24个核苷酸,更优选长度为21、22或23个核苷酸。
RNAi介导的基因沉默的另一个途径源自于内源性编码的原始微型RNA(pri-miRNA)转录本,它在细胞中被加工以产生前体miRNA(pre-miRNA)。这些miRNA分子从细胞核输出到细胞质中,在这里经历加工以产生成熟的miRNA分子(miRNA),它们通过识别mRNAs的3’非翻译区中的靶位点,然后通过加工性P-体降解mRNA,来直接抑制翻译(综述在Kim&Rossi,Nature Rev.Genet.8:173-204(2007)中)。
RNAi的临床应用包括掺入合成的siRNA双链体,它们优选大小为20-23个核苷酸,并优选具有2个核苷酸的3’重叠。基因表达的降低是通过靶mRNA的序列特异性设计建立起来的。几个用于这种分子的最适设计和合成的商业化位点对于本技术领域的专业人员来说是已知的。
其它的应用提供了较长的siRNA分子(典型长度为25-30个核苷酸,优选为大约27个核苷酸),以及小的发夹RNAs(shRNAs;典型长度为大约29个核苷酸)。后者是天然表达的,描述在Amarzguioui等(FEBS Lett.579:5974-81(2005))中。化学合成的siRNAs和shRNAs是体内加工的底物,在某些情况下提供了比较短的设计更强有力的基因沉默(Kim等,Nature Biotechnol.23:222-226(2005);Siolas等,NatureBiotechnol.23:227-231(2005))。一般来说,siRNAs提供暂时的基因表达沉默,因为它们的细胞内浓度被随后的细胞分裂稀释了。相反,表达的shRNAs介导长期的、稳定的靶转录本的降低,只要shRNA的转录发生就行(Marques等,Nature Biotechnol.23:559-565(2006);Brummelkamp等,Science 296:550-553(2002))。
因为RNAi分子、包括siRNA、miRNA和shRNA、以序列依赖性的方式起作用,因此本发明的变体(例如本文描述的标记物和单倍型)可用于设计RNAi试剂,它们识别含有特定等位基因和/或单倍型(例如本发明的等位基因和/或单倍型)的特定核酸分子,同时不识别含有其它等位基因或单倍型的核酸分子。因此,这些RNAi试剂可以识别并破坏靶核酸分子。与反义试剂相同,RNAi试剂可用作治疗性药剂(即用于关闭与疾病相关的基因或与疾病相关的基因的变体),但是也可以用于对基因功能进行表征和证实(例如通过基因敲除或基因击落实验)。
RNAi的递送可以通过本技术领域的专业人员已知的各种方法来进行。使用非病毒递送的方法包括胆固醇、稳定的核酸-脂类颗粒(SNALP)、重链抗体片段(Fab)、适体和纳米颗粒。病毒递送的方法包括使用慢病毒、腺病毒和腺相关病毒。在某些实施方案中,siRNA分子被化学修饰以增加它们的稳定性。这可以包括在核糖的2’位置的修饰,包括2’-O-甲基嘌呤和2’-氟代嘧啶,它们提供了对Rnase活性的抗性。其它的化学修饰也是可能的,并为本技术领域的专业人员所知。
下面的参考文献为RNAi以及使用RNAi靶向特定基因的可能性提供了进一步的概述:Kim & Rossi,Nat.Rev.Genet.8:173-184(2007),Chen & Rajewsky,Nat.Rev.Genet.8:93-103(2007),Reynolds等,Nat.Biotechnol.22:326-330(2004),Chi等,Proc.Natl.Acad.Sci.USA100:6343-6346(2003),Vickers等,J.Biol.Chem.278:7108-7118(2003),Agami,Curr.Opin.Chem.Biol.6:829-834(2002),Lavery等,Curr.Opin.Drug Discov.Devel.6:561-569(2003),Shi,Trends Genet.19:9-12(2003),Shuey等,Drug Discov.Today 7:1040-46(2002),McManus等,Nat.Rev.Genet.3:737-747(2002),Xia等,Nat.Biotechnol.20:1006-10(2002),Plasterk等,Curr.Opin.Genet.Dev.10:562-7(2000),Bosher等,Nat.Cell Biol.2:E31-6(2000)和Hunter,Curr.Biol.9:R440-442(1999)。
导致发生疾病包括2型糖尿病的增加的倾向性或风险的遗传缺陷、或引起疾病的缺陷,可以通过给带有缺陷的对象施用含有修复序列的核酸片段,在遗传缺陷的位点处提供正常的/野生型核苷酸,来永久地校正。这样的位点特异性修复序列可以包含操作以促进对象的基因组DNA的内源修复的RNA/DNA寡核苷酸。修复序列的施用可以通过适当的载体来进行,例如与聚乙烯亚胺复合、囊封在阴离子脂质体中、病毒载体例如腺病毒载体、或其它适合于促进施用的核酸的细胞内摄入的药物组合物。然后可以克服遗传缺陷,因为嵌合的寡核苷酸诱导正常的序列整合到对象的基因组中,导致正常的/野生型基因产物的表达。替换是可繁殖的,因此提供了永久性的修复和与疾病或病症有关的症状的缓解。
本发明提供了鉴定可用于治疗2型糖尿病的化合物或药剂的方法。因此,本发明的变体可用作鉴定和/或开发治疗性药剂的靶。这样的方法可以包括分析药剂或化合物调节含有至少一个本发明的变体(标记物和/或单倍型)的核酸或核酸编码的产物的活性和/或表达的能力。这反过来可用于鉴定抑制或改变编码的核酸产物的不需要的活性或表达的药剂或化合物。用于执行这样的实验的分析方法可以在专业技术人员已知的基于细胞的系统或无细胞系统中进行。基于细胞的系统包括天然表达目标核酸分子的细胞,或已经被遗传修饰以表达某些所需核酸分子的重组细胞。
在患者中变体的基因表达可以通过含有变体的核酸序列的表达(例如含有本发明的至少一个变体的基因,它可以被转录成含有至少一个变体的RNA,并进一步翻译成蛋白)、或通过由于变体影响了正常转录本的表达水平或形式、例如在基因的调节或控制区域中的变体而导致的正常/野生型核酸序列的改变的表达,来进行评估。用于基因表达的分析方法包括直接的核酸分析(mRNA)、表达的蛋白水平的分析、或参与途径例如信号途径的并行化合物的分析。此外,对于信号途径作出响应而被上调或下调的基因的表达,也可以被分析。一个实施方案包括了将报告基因例如荧光素酶与目标基因的调控区可操作连接。
在一个实施方案中,将细胞与候选化合物或药剂相接触,并测定mRNA的表达,可以鉴定基因表达的调节剂。将存在候选化合物或药剂的情况下mRNA的表达水平与不存在化合物或药剂的情况下的表达水平进行比较。根据这种比较,用于治疗2型糖尿病的候选化合物或药剂可以被鉴定为调节变体基因的基因表达的化合物或药剂。当在存在候选化合物或药剂的情况下与不存在它的情况下相比,mRNA或编码的蛋白的表达统计学显著地增加时,候选化合物或药剂被鉴定为核酸表达的刺激剂或上调物。当在存在候选化合物或药剂的情况下与不存在它的情况下相比,核酸的表达或蛋白水平统计学显著地降低时,候选化合物被鉴定为核酸表达的抑制剂或下调物。
本发明还提供了使用通过药物(化合物和/或药剂)筛选鉴定到的化合物作为基因调节剂(即基因表达的刺激剂和/或抑制剂)来进行治疗的方法。
本发明的另一方面,提供了药物包(试剂盒),药物包包含治疗性药剂,以及一套本文公开的给人类施用治疗性药剂以诊断性测试本发明的一种或多种变体的说明书。治疗性药剂可以是小分子药物、抗体、肽、反义或RNAi分子,或其它治疗性分子。在一个实施方案中,被鉴定为本发明的至少一种变体的携带者的个体被要求服用处方剂量的治疗性药剂。在一个这样的实施方案中,被鉴定为本发明的至少一个变体的纯合的携带者的个体被要求服用处方剂量的治疗性药剂。在另一个实施方案中,被鉴定为本发明的至少一个变体的非携带着的个体被要求服用处方剂量的治疗性药剂。
评估对治疗性药剂响应的可能性的方法、监测治疗的进展的方法以及治疗的方法
正如本技术领域已知的,个体对具体的疗法(例如治疗性药剂或治疗方法)可能有不同的响应。药物基因组学致力于由于药物特性的改变和/或药物作用的异常或变化,遗传变异(例如本发明的变体(标记物和/或单倍型))是如何影响药物响应的问题。因此,响应差异的基础可能部分是由遗传决定的。由于遗传变异影响药物响应而产生的临床结果,在某些个体中(例如本发明的遗传变体的携带者或非携带者)可能导致药物的毒性或药物的治疗失败。因此,本发明的变体可以确定治疗性药剂和/或方法作用于身体的方式,或者身体代谢治疗性药剂的方式。
因此,在一个实施方案中,多态性位点上特定等位基因或单倍型的存在是对特定治疗方式的不同、例如不同响应率的指示。这意味着被诊断患有2型糖尿病或携带有本发明的多态性位点处的某些等位基因或单倍型(例如本发明的有风险和保护性等位基因和/或单倍型)的患者,将对特定的治疗、药物和/或用于治疗疾病的其它疗法具有较好的、或较糟的响应。因此,标记物等位基因或单倍型的存在或不存在,可以帮助确定对患者应该使用什么疗法。例如,对于新诊断的患者来说,可以评估本发明的标记物或单倍型的存在(例如如本文描述的通过测试源自于血液样品的DNA)。如果患者对标记物等位基因或单倍型阳性(也就是说存在标记物的至少一个特定等位基因或单倍型),那么医生可以推荐一种特定的疗法,而如果患者对标记物的至少一个等位基因或单倍型阴性,那么可以推荐不同的疗法过程(可以包括推荐进行除了连续监测疾病的进展之外,不进行即时的疗法)。因此,患者的携带者状态可用于帮助确定是否应该进行特定的治疗方式。值位于能够在早期阶段诊断疾病的可能性的范围内,以选择最适合的疗法,并给临床医生提供关于疾病的预后/攻击性的信息,以便能够使用最适合的疗法。
在某些实施方案中,治疗方式包括施用药剂表1和药剂表2中显示的至少一种治疗药剂。在一个实施方案中,治疗药剂选自双胍类、噻唑烷二酮类、磺酰脲类、格列奈类、α-葡萄糖苷酶抑制剂和DPP-4抑制剂。在一个实施方案中,双胍是二甲双胍或二甲双胍加格列本脲。其它的包含二甲双胍的组合疗法、包括与噻唑烷二酮类的组合,也考虑到了,并在本发明的范围内。在另一个实施方案中,磺酰脲选自乙酰磺酰环己脲、氯磺丙脲、格列其特达美康、格列美脲、格列吡嗪、格列本脲、妥拉磺脲和甲苯磺胺丁脲。在另一个实施方案中,噻唑烷二酮选自吡格列酮、罗格列酮和mitoglitazone,或其它噻唑烷二酮衍生物。在另一个实施方案中,治疗药剂选自药剂表2中显示的药剂。
本发明还涉及监测对2型糖尿病的治疗的进展或有效性的方法。这可以根据本发明的标记物和单倍型的基因型和/或单倍型状态来进行,即通过评估本文公开的至少一个多态性标记物的至少一个等位基因的不存在或存在,或通过监测本发明的与变体(标记物和单倍型)相关的基因的表达来进行。可以测量组织样品(例如外周血样品或活检样品)中风险基因的mRNA或编码的多肽。因此,在治疗之前或期间可以确定表达水平和/或mRNA水平,以监测其有效性。可选地或同时地,在治疗之前或期间可以如本文公开地测定至少一个2型糖尿病的风险变体的基因型和/或单倍型状态,以监测其有效性。可选地,可以通过测定mRNA和/或多肽水平,来监测与本发明的标记物和单倍型有关的生物网络或代谢途径。这可以通过例如在治疗之前和期间在所取的样品中监测属于网络和/或途径的几个基因的表达水平或多肽来进行。或者,在治疗之前和期间可以测定属于生物网络或代谢途径的代谢物。通过将治疗过程中观察到的表达水平/代谢物水平的变化与健康对象的相应数据进行比较,可以确定治疗的有效性。
因此,根据个体的基因型状态,在携带有被发现与2型糖尿病的增加的易感性或风险相关的至少一个标记物的至少一个风险等位基因的个体中,监测了疗法的进展。携带有本文描述的风险变体的个体可以得益于比非携带者更密切或更频繁地监测疗法的进展,可选地与上面描述的特定的治疗方式或使用的治疗药剂相组合。
另一方面,本发明的标记物可用于增加临床试验的能力和有效性。因此,是本发明的至少一个风险变体的携带者的个体、即是赋予发生2型糖尿病的增加的风险的至少一个多态性标记物的至少一个等位基因的携带者的个体,可能更可能对特定的治疗方式做出响应。在一个实施方案中,携带有特定的治疗(例如小分子药物)所靶向的途径和/或代谢网络中的基因的风险变体的个体,更可能是治疗的响应者。在另一个实施方案中,携带有其表达和/或功能被风险变体改变的基因的风险变体的个体,更可能是靶向该基因、其表达或其基因产物的治疗方式的响应者。本应用可以提高临床试验的安全性,但是也可以增加临床试验证明出统计学显著的效能的机会,这种效能在群体的某些亚组中可能是有限的,例如是本文描述的风险变体的携带者或非携带者的个体。因此,这种试验的一个可能的结果是,某些遗传变体例如本发明的标记物和单倍型的携带者,统计学显著地可能显示出对治疗性药剂的阳性响应,即当按照处方服用治疗性药剂或药物时,经历了与2型糖尿病有关的症状的缓解。
另一方面,本发明的标记物和单倍型可用于指导对特定个体的药物试剂的选择。利用本发明的风险变体,可以实现治疗方式的个性化选择、生活方式的改变或二者的组合。因此,关于本发明的特定标记物的个体状态的信息,在靶基因或基因产物受到本发明的风险变体影响的情况下,可用于选择治疗选项。变体的某些组合可能适合于治疗选项的一种选择,而其它基因变体组合可以靶向其它治疗选项。根据需要,这样的变体组合可以包括一种变体、两种变体、三种变体或四种或四种以上的变体,以临床可靠的精确性确定治疗模式的选择。
除了本发明的变体的诊断和治疗应用之外,变体(标记物和单倍型)也可以是用于人类身份鉴定的有用标记物,可用于法医学、亲子鉴定和生物计量学。SNPs在法医学领域的具体应用由Gill(Int.J.LegalMed.114:204-10(2001))进行了综述。个体之间基因组DNA的遗传差异可用作遗传标记物以鉴定个体,并将生物样品与个体相关联。遗传标记物、包括SNPs和微卫星,可用于鉴别个体。分析的标记物越多,在任何给定的个体中标记物的等位基因组合与无关个体(假设标记物是无关的,即标记物是完美的连锁平衡的)中相同的可能性越低。因此,用于这些目的的变体优选是无关的,即它们是独立遗传的。因此,优选的标记物可以选自可用的标记物,例如本发明的标记物,选择的标记物可以含有来自人类基因组不同区域的标记物,包括不同染色体上的标记物。
在某些应用中,用于法医测试的SNPs来自简并密码子位置(即某些密码子的第三个位置,以便SNP的变异不影响密码子编码的氨基酸)。在其它应用中、例如预测基因型特征包括种族、血统或身体特征的应用中,利用影响编码的蛋白的氨基酸序列的SNPs可能是有用的或需要的。在其它这样的实施方案中,变体(SNP或其它多态性标记物)影响了邻近基因的表达水平,从而导致了改变的蛋白表达。
本发明还涉及本文描述的与2型糖尿病有关联的多态性标记物和单倍型的计算机执行的应用。这样的应用可以用于储存、操作或分析在本发明的方法中有用的基因型数据。一个例子涉及在可读介质上储存来自于个体的基因型信息,以便能够给第三方(例如个体)提供基因型信息,或用于从基因型数据衍生信息,例如通过将基因型数据与关于对2型糖尿病的增加的易感性有贡献的遗传风险因子的信息进行比较,并在这些比较的基础上报告结果。
一种这样的情况涉及计算机可读介质。一般来说,这样的介质有能力储存(i)至少一个多态性标记物或单倍型的识别信息;(ii)在患有2型糖尿病的个体中该至少一个标记物的至少一个等位基因的频率、或单倍型的频率的指示符;以及在参比群体中该至少一个标记物的至少一个等位基因的频率、或单倍型的频率的指示符。参比群体可以是无疾病的个体的群体。可选地,参比群体是来自总群体的随机样品,因此充分地代表了群体。频率指示符可以是计算出的频率、等位基因和/或单倍型拷贝的计数、或实际频率的归一化的或操作过的值,它们适合用于特定的介质。
其它关于个体的信息也可以储存在介质上,例如血统信息、性别信息、物理属性或特征(包括身高和体重)、生物化学测量值(例如血压、血脂水平、空腹葡萄糖水平、胰岛素反应测量值),或其它希望与特定个体的基因型状态一起储存或操作的有用信息。
本发明还涉及适合用于测定或操作遗传数据的装置,这些遗传数据可用于确定人类个体中对2型糖尿病的易感性。这样的装置可以包含计算机可读存储器,以及储存在计算机可读存储器中的用于操作数据的例行程序,以及用于产生含有遗传数据的测量值的输出的例行程序。这样的测量值可以包括例如等位基因或单倍型频率、基因型计数、性别、年龄、表现型信息的值,比值比(OR)或相对风险(RR)、群体可归因风险(PAR)的值,或其它作为原始基因型数据的直接统计数据或基于遗传数据的计算值的有用信息。
上述的应用都可以使用本发明的标记物和单倍型来进行实践,这些标记物和单倍型已经在评估对2型糖尿病的易感性的方法中进行了更详细的描述。因此,一般来说,这些应用可以被减少到使用表1-6中列出的标记物、以及与其连锁不平衡的标记物、例如表22、23和24中显示的标记物,来进行实施。在一个实施方案中,标记物或单倍型出现在其序列显示在SEQ ID NO:1、SEQ ID NO:2或SEQ ID NO:3中的基因组区段中。在另一个实施方案中,标记物和单倍型包括选自下列标记物的至少一种标记物:rs2497304(SEQ ID NO:16)、rs947591(SEQ ID NO:30)、rs10882091(SEQ ID NO:4)、rs7914814(SEQ IDNO:24)、rs6583830(SEQ ID NO:20)、rs2421943(SEQ ID NO:15)、rs6583826(SEQ ID NO:19)、rs7752906(SEQ ID NO:32)、rs1569699(SEQ ID NO:6)、rs7756992(SEQ ID NO:21)、rs9350271(SEQ IDNO:33)、rs9356744(SEQ ID NO:34)、rs9368222(SEQ ID NO:35)、rs10440833(SEQ ID NO:36)、rs6931514(SEQ ID NO:37)、rs1860316(SEQ ID NO:10)、rs1981647(SEQ ID NO:11)、rs1843622(SEQ IDNO:9)、rs2191113(SEQ ID NO:13)和rs9890889(SEQ ID NO:31),可选地包括与其连锁不平衡的标记物,其中连锁不平衡由r2的数值大于0.2来定义。在另一个实施方案中,标记物或单倍型含有选自下列标记物的至少一个标记物:rs2497304等位基因A、rs947591等位基因A、rs10882091等位基因C、rs7914814等位基因T、rs6583830等位基因A、rs2421943等位基因G、rs6583826等位基因G、rs7752906等位基因A、rs1569699等位基因C、rs7756992等位基因G、rs9350271等位基因A、rs9356744等位基因C、rs9368222等位基因A、rs10440833等位基因A、rs6931514等位基因G、rs1860316等位基因A、rs1981647等位基因C、rs1843622等位基因T、rs2191113等位基因A和rs9890889等位基因A。在另一个实施方案中,至少一个标记物或单倍型含有选自表22、23和24中显示的标记物的至少一个标记物。
核酸和多肽
本文描述的核酸和多肽可用于诊断对2型糖尿病的易感性的方法,以及用于这样的诊断的试剂盒。
本文使用的“分离”的核酸分子,是与正常情况下位于基因或核苷酸序列两侧(例如在基因组序列中)的核酸分开的,和/或已经从其它转录的序列中(例如在RNA文库中)完全或部分纯化出的核酸分子。例如,本发明的分离的核酸,相对于它自然发生的复杂的细胞环境、或通过重组技术生产时的培养基、或化学合成时的化学前体或其它化学物质来说,可以是基本上分离的。在某些情况下,分离的物质将形成组合物(例如含有其它物质的粗提液)、缓冲系统或试剂混合物的一部分。在其它情况下,物质可以被纯化到例如通过聚丙烯酰胺凝胶电泳(PAGE)或柱层析(例如HPLC)测定时基本上均一。本发明的分离的核酸分子可以含有所有存在的大分子物质的至少大约50%、至少大约80%或至少大约90%(摩尔数的基础上)。对于基因组DNA来说,术语“分离的”也可以指从与基因组DNA天然关联的染色体分离出的核酸分子。例如,分离的核酸分子可以含有少于大约250kb、200kb、150kb、100kb、75kb、50kb、25kb、10kb、5kb、4kb、3kb、2kb、1kb、0.5kb或0.1kb的、在核酸分子所源自的细胞的基因组DNA中位于核酸分子侧翼的核苷酸。
核酸分子可以与其它编码或调节序列融合,并仍被当作是分离的。因此,载体中包含的重组DNA包含在本文使用的“分离的”的定义内。分离的核酸分子也包含异源宿主细胞或异源生物体中的重组DNA分子,以及溶液中的部分纯化的或基本上纯化的DNA分子。“分离的”核酸分子也包括本发明的DNA分子的体外和体内RNA转录本。分离的核酸分子或核苷酸序列可以包括化学合成的或通过重复方法合成的核酸分子或核苷酸序列。这样的分离的核苷酸序列可用于例如编码的多肽的制造、作为探针用于分离同源序列(例如从其它哺乳动物物种)、用于基因作图(例如通过与染色体原位杂交)、或用于检测组织(例如人类组织)中基因的表达,例如通过Northern印迹分析或其它杂交技术。
本发明还涉及在高度严紧杂交条件下、例如用于选择性杂交的条件下,与本文描述的核苷酸序列杂交的核酸分子(例如与含有本文描述的单倍型相关的多态性位点的核苷酸序列特异性杂交的核酸分子)。在一个实施方案中,本发明包含了在在高度严紧杂交和清洗条件(例如用于选择性杂交的条件)下,与含有LD区块C06(SEQ ID NO:1)、LD区块C10(SEQ ID NO:2;例如编码IDE、KIF11和/或HHEX基因的核苷酸序列)和LD区块C17(SEQ ID NO:3)、或CDKAL1基因或其片段的核苷酸序列的核苷酸序列(或含有LD区块C06(SEQ ID NO:1)、LD区块C10(SEQ ID NO:2;例如编码IDE、KIF11和/或HHEX基因的核苷酸序列)和LD区块C17(SEQ ID NO:3)、或CDKAL1基因或其片段的核苷酸序列的互补序列的核苷酸序列)杂交的变体,其中核苷酸序列包括含有在本文描述的单倍型(例如单倍型)中的至少一个多态性等位基因。
这样的核酸分子可以通过等位基因特异性或序列特异性杂交(例如在高度严紧条件下)来检测和/或分离。用于核酸杂交的严紧条件和方法被解释在《分子生物学现代方法》(Current Protocols in MolecularBiology)的2.10.1-2.10.16页和6.3.1-6.3.6页中(Ausubel,F.等,《分子生物学现代方法》,(Current Protocols in Molecular Biology),JohnWiley & Sons,(1998)),以及Kraus,M.和Aaronson,S.,MethodsEnzymol.,200:546-556(1991)中,在此以其全文引为参考。
两个核苷酸或氨基酸序列的百分同一性可以通过出于最适比较的目的将序列进行比对(例如可以在第一个序列的序列中引入缺口)来确定。然后比较相应位置上的核苷酸或氨基酸,两个序列之间的百分同一性是序列共有的同样的位置的数量的函数(即%同一性=同一的位置的数量/总的位置的数量x100)。在某些实施方案中,出于比较目的进行比对的序列的长度是参比序列的长度的至少30%、至少40%、至少50%、至少60%、至少70%、至少80%、至少90%或至少95%。两个序列的实际比较可以通过众所周知的方法来进行,例如使用数学算法。这样的数学算法的非限制性的例子描述在Karlin,S.和Altschul,S.,Proc.Natl.Acad.Sci.USA,90:5873-5877(1993)中。这样的算法被整合在NBLAST和XBLAST程序(2.0版)中,描述在Altschul,S.等,Nucleic Acids Res.,25:3389-3402(1997)中。在使用BLAST和带缺口BLAST程序时,可以使用相应程序(例如NBLAST)的缺省参数。参见万维网ncbi.nlm.nih.gov网点。在一个实施方案中,用于序列比较的参数可以被设置为分值=100,字长=12,或可以是不同的(例如W=5或W=20)。
其它的例子包括了Myers和Miller,CABIOS(1989)的算法,在Torellis,A.和Robotti,C.,Comput.Appl.Biosci.10:3-5(1994)中描述的ADVANCE和ADAM算法;以及在Pearson,W.和Lipman,D.,Proc.Natl.Acad.Sci.USA,85:2444-48(1988)中描述的FASTA。在另一个实施方案中,两个氨基酸序列之间的百分同一性可以使用GCG软件包(Accelrys,Cambridge,UK)中的GAP程序来完成。
本发明还提供了分离的核酸分子,它们含有的片段或部分在高度严紧条件下,与含有或包含LD区块C06(SEQ ID NO:1)、LD区块C10(SEQ ID NO:2;例如编码IDE、KIF11和/或HHEX基因的核苷酸序列)和LD区块C17(SEQ ID NO:3)、或CDKAL1基因或其片段的核苷酸序列(或含有或包含LD区块C06(SEQ ID NO:1)、LD区块C10(SEQ ID NO:2;例如编码IDE、KIF11和/或HHEX基因的核苷酸序列)和LD区块C17(SEQ ID NO:3)、或CDKAL1基因或其片段的核苷酸序列的互补序列的核苷酸序列)的核酸杂交,其中核苷酸序列含有本文描述的单倍型(例如单倍型)中包含的至少一个多态性等位基因。本发明的核酸片段的长度为至少大约15、至少大约18、20、23或25个核苷酸,可以是30、40、50、100、200、500、1000、10,000个或以上的核苷酸长。
本发明的核酸片段在例如本文描述的分析方法中被用作探针或引物。“探针”或“引物”是以碱基特异性的方式与核酸分子的互补链杂交的寡核苷酸。除了DNA和RNA之外,这样的探针和引物包括多肽核酸(PNA),它描述在Nielsen,P.等,Science 254:1497-1500(1991)中。探针或引物含有与核酸分子的至少大约15个、典型为大约20-25个、在某些实施方案中大约40、50或75个连续的核苷酸杂交的核苷酸序列区域。在一个实施方案中,探针或引物含有本文描述的至少一个多态性标记物的至少一个等位基因或至少一个单倍型,或其互补序列。在特定实施方案中,探针或引物可以含有100个或以下的核苷酸;例如,在某些实施方案中从6到50个核苷酸,或例如从12到30个核苷酸。在其它实施方案中,探针或引物与连续的核苷酸序列或连续的核苷酸序列的互补序列至少70%同一、至少80%同一、至少85%同一、至少90%同一或至少95%同一。在另一个实施方案中,探针或引物能够与连续的核苷酸序列或连续的核苷酸序列的互补序列选择性杂交。通常情况下,探针或引物还含有标记,例如放射性同位素、荧光标记、酶标记、酶辅助因子标记、磁性标记、自旋标记、表位标记。
本发明的核酸分子、例如本文描述的那些,可以使用本领域技术人员熟知的标准分子生物学技术来鉴定和分离。可以将扩增的DNA标记(例如放射性标记)并用作探针,筛选来自人类细胞的cDNA文库。cDNA可以源自于mRNA并包含在适合的载体中。可以分离到相应的克隆,在体内切除后可以获得DNA,然后可以通过本技术领域现有的方法在任一或两个方向上对克隆的插入片段进行测序,以鉴定编码具有适合分子量的多肽的正确的阅读框架。使用这些或类似的方法,可以对多肽和编码多肽的DNA进行分离、测序和进一步表征。
一般来说,本发明的分离的核酸序列在Southern凝胶中可以用作分子量标准品,并作为被标记的染色体标记物以对相关基因位置进行作图。核酸序列也可用于与患者中的内源DNA序列进行比较,以鉴定2型糖尿病或对2型糖尿病的易感性,以及作为探针例如用于杂交和发现相关的DNA序列或从样品中扣除已知的序列(例如扣除杂交)。核酸序列还可以用于产生引物,用于遗传指纹法、使用免疫技术产生抗多肽抗体、和/或作为抗原产生抗DNA抗体或引发免疫应答。
抗体
本发明还提供了特异性结合一种形式的基因产物但是不结合另一种形式的基因产物的多克隆抗体和/或单克隆抗体。还提供了与含有多态性位点或多个位点的变体或参比基因产物的一部分结合的抗体。本文使用的术语“抗体”是指免疫球蛋白分子和免疫球蛋白的免疫活性部分,即含有与抗原特异性结合的抗原结合位点的分子。与本发明的多肽特异性结合的分子,是与该多肽或其片段结合、但是与样品、例如天然含有多肽的生物学样品中的其它分子基本上不结合的分子。免疫球蛋白分子的免疫活性部分的例子包括F(ab)和F(ab’)2片段,可以通过用酶例如胃蛋白酶处理抗体来产生。本发明提供了与本发明的多肽结合的多克隆和单克隆抗体。本文使用的术语“单克隆抗体”或“单克隆抗体组合物”是指一群抗体分子,只含有一种能够与本发明的多肽的特定表位发生免疫反应的抗原结合位点。因此,单克隆抗体组合物典型地表现出对与其发生免疫反应的本发明的特定多肽的单一结合亲和性。
多克隆抗体可以按照以前的描述通过用所需的免疫原例如本发明的多肽或其片段免疫适当的对象来制备。可以通过标准的技术随时间监测被免疫的对象中的抗体滴度,例如使用固定化的多肽的酶联免疫吸附分析(ELISA)。如果需要,可以从哺乳动物(例如从血液)中分离针对多肽的抗体分子,并通过众所周知的技术例如蛋白A层析进一步纯化以获得IgG级份。在免疫后适当的时间,例如当抗体滴度最高时,可以从对象获得抗体生产细胞,用于通过标准技术制备单克隆抗体,例如最初由Kohler和Milstein,Nature 256:495-497(1975)描述的杂交瘤技术、人类B细胞杂交瘤技术(Kozbor等,Immunol.Today 4:72(1983))、EBV杂交瘤技术(Cole等,《单克隆抗体与癌症疗法》(Monoclonal Antibodies and Cancer Therapy),Alan R.Liss,1985,Inc.,pp.77-96)或trioma技术。用于生产杂交瘤的技术是众所周知的(一般来说,参见《免疫学现代方法》(Current Protocols in Immunology)(1994)Coligan等主编,John Wiley & Sons,Inc.,New York,NY)。简单来说,将永生的细胞株(典型为骨髓瘤)与用来自上述的免疫原免疫的哺乳动物的淋巴细胞(典型为脾细胞)融合,并筛选获得的杂交瘤细胞的培养上清液,以鉴定产生与本发明的多肽结合的单克隆抗体的杂交瘤。
多种众所周知的用于融合淋巴细胞和永生细胞株的方案中的任何一种都可用于产生针对本发明的多肽的单克隆抗体(参见例如《免疫学现代方法》(Current Protocols in Immunology),同上;Galfre等,Nature 266:55052(1977);R.H.Kenneth,Monoclonal Antibodies:A NewDimension In Biological Analyses,Plenum Publishing Corp.,New York,New York(1980);以及Lerner,Yale J.Biol.Med.54:387-402(1981))。此外,普通技术人员将会认识到,这种方法的许多变化形式也将可以使用。
或者,为了制备分泌单克隆抗体的杂交瘤,可以通过用多肽筛选重组组合免疫球蛋白文库(例如抗体噬菌体展示文库),从而分离与多肽结合的免疫球蛋白文库成员,来鉴定和分离针对本发明的多肽的单克隆抗体。用于产生和筛选噬菌体展示文库的试剂盒是可商购的(例如Pharmacia的重组噬菌体抗体系统,目录号No.27-9400-01;以及Stratagene SurfZAPTM噬菌体展示试剂盒,目录号No.240612)。此外,特别适合用于产生和筛选抗体展示文库的方法和试剂的例子可以在下列文献中发现,例如美国专利No.5,223,409,PCT公开号WO92/18619,PCT公开号WO 91/17271,PCT公开号WO 92/20791,PCT公开号WO 92/15679,PCT公开号WO 93/01288,PCT公开号WO92/01047,PCT公开号WO 92/09690,PCT公开号WO 90/02809,Fuchs等,Bio/Technology 9:1370-1372(1991);Hay等,Hum.Antibod.Hybridomas 3:81-85(1992),Huse等,Science 246:1275-1281(1989),和Griffiths等,EMBO J.12:725-734(1993)。
此外,可以使用标准的重组DNA技术制造的、含有人类和非人类部分的重组抗体、例如嵌合和人源化单克隆抗体,在本发明的范围内。这样的嵌合和人源化单克隆抗体可以通过本技术领域已知的重组DNA技术来生产。
总的来说,抗体(例如单克隆抗体)可用于通过标准技术、例如亲和层析或免疫沉淀,来分离本发明的多肽。多肽特异性抗体可以便于从细胞纯化天然的多肽以及在宿主细胞中表达的重组产生的多肽。此外,特异性针对本发明的多肽的抗体,可用于检测多肽(例如细胞裂解物、细胞上清液或组织样品中的),以评估多肽表达的丰度和图谱。作为临床测试步骤的一部分,抗体在诊断上可用于监测组织中的蛋白水平,以例如确定给定治疗方案的效能。抗体可以与可检测物质结合,以便于其检测。可检测的物质的例子包括各种不同的酶、辅基、荧光物质、发光物质、生物发光物质和放射性物质。适合的酶的例子包括辣根过氧化物酶、碱性磷酸酶、β-半乳糖苷酶或乙酰胆碱酯酶;适合的辅基复合物的例子包括链亲和素/生物素和亲和素/生物素;适合的荧光物质的例子包括7-羟基香豆素(umbelliferone)、荧光素、荧光素异硫氰酸酯、罗丹明、dichlorotriazinylamine荧光素、丹磺酰氯或藻红蛋白;发光材料的例子包括鲁米诺(luminol);生物发光材料的例子包括荧光素酶、荧光素和水母发光蛋白,适合的放射性材料的例子包括125I、131I、35S或3H。
抗体也可用于药物基因组学分析。在这样的实施方案中,针对本文描述的核酸编码的变体蛋白、例如由含有本发明的至少一个多态性标记物的核酸编码的变体蛋白的抗体,可用于鉴定需要修改治疗模式的个体。
此外,抗体可用于评估在疾病状态中、例如在2型糖尿病的活动阶段中,或者在具有2型糖尿病的倾向性的个体中,与蛋白的功能相关的变体蛋白的表达。特异性针对由含有本文描述的至少一个多态性标记物或单倍型的核酸编码的本发明的变体蛋白的抗体,可用于筛选变体蛋白的存在,例如用于筛选由变体蛋白的存在所指示的对2型糖尿病的倾向性。
抗体可以用于其它方法中。因此,抗体可以用作诊断工具与电泳迁移率、等电点、胰蛋白酶或其它蛋白酶消化分析一起使用,用于评估蛋白、例如本发明的变体蛋白,或用于本技术领域的专业人员已知的其它物理分析中。抗体也可用于组织分型。在一个这样的实施方案中,特定的变体蛋白与在特定组织类型中的表达相关联,然后可以使用特异性针对变体蛋白的抗体来鉴定特定的组织类型。
蛋白、包括变体蛋白的亚细胞定位,也可以使用抗体来确定,并且可用于评估各种不同组织的细胞中蛋白的异常亚细胞定位。这样的用途可以用于遗传测试中,但是也可以用于监测特定的治疗模式。在治疗的目的是校正变体蛋白的表达水平或存在、或变体蛋白的异常组织分布或发育表达的情况下,可以使用特异性针对变体蛋白或其片段的抗体来监测治疗的效能。
抗体还可用于抑制变体蛋白的功能,例如通过阻断变体蛋白与结合分子或配偶体的结合。这样的用途也可用于涉及了抑制变体蛋白的功能的治疗的情况中。例如,抗体可用于阻断或竞争性抑制结合,从而调节(即激活或拮抗)蛋白的活性。可以制备针对含有特定功能所需的位点的特定蛋白片段的抗体,或针对与细胞或细胞膜有关的完整蛋白的抗体。为了在体内施用,可以将抗体与其它的治疗有效荷载物连接,例如放射性核素、酶、免疫原性表位或细胞毒性剂、包括细菌毒素(白喉毒素或植物毒素,例如篦麻毒素)。抗体或其片段的体内半衰期可以通过与聚乙二醇连接进行PEG化而增加。
现在,将通过下面的非限制性的实施例对本发明进行举例说明。
实施例
实施例1
下面描述了通过SNP标记物的单点和单倍型分析来鉴定被发现与2型糖尿病相关的易感性因子。
方法
冰岛组
冰岛数据保护局和冰岛国家生物伦理委员会批准了本研究。研究中的所有参加者给出了书面同意书。所有与血液样品、医疗信息和系谱学相关的个人标识符首先由数据保护局使用第三方加密系统进行加密。
对于本研究来说,鉴定了2400个2型糖尿病患者,他们或是过去30年间通过在冰岛心脏联合会进行的长期流行病学研究中诊断的,或是过去12年间在雷克雅未克(Reykjavik)的两所主要医院的一所中诊断的。这些患者中有三分之二是活着的,代表了今天冰岛已知的2型糖尿病患者群体的大约一半。为了进行本研究,接触了这些患者中的大多数,合作率超过80%。所有本研究的参与者拜访了冰岛心脏联合会,在那里他们回答了调查问卷,进行了抽血和空腹血浆葡萄糖测定。问题中包含了关于药物治疗和诊断时的年龄。按照我们以前发表的连锁研究中的描述(Reynisdottir等,Am J Hum Genet 73,323(2003))对本研究中的2型糖尿病患者进行了诊断。简单来说,由本研究的医生通过以前的医学记录、药物治疗史和/或新的实验室测量,对2型糖尿病的诊断进行了证实。对于以前诊断的2型糖尿病患者来说,使用口服降糖药剂的报告证实了2型糖尿病。目前正使用胰岛素进行治疗的个体,如果他们也使用或以前使用过口服降糖药剂,则被分类为患有2型糖尿病。在该组中,大部分进行药物治疗的患者服用口服降糖药剂,只有少部分(9%)需要胰岛素。对于迄今未被诊断的个体来说,2型糖尿病和受损的空腹葡萄糖(IFG)的诊断是根据美国糖尿病学会设定的标准(糖尿病诊断和分类专家委员会1997)。本研究中的2型糖尿病患者的平均年龄是69.7岁。
重复组
丹麦研究组是一组2型糖尿病患者,他们来自于哥本哈根的Steno糖尿病中心(N=1,018),以及生活在大哥本哈根地区、并在预防和卫生研究中心28取样的30-60岁个体的基于Inter99群体的样品(N=359)。糖尿病和糖尿病前期范畴的诊断按照1999年世界卫生组织(WHO)的标准。从Inter99收藏库获得了具有BMI测量值的丹麦对照组的有效随机亚组(N=2,400)。在参加之前,从所有对象获得了正式的书面同意书。研究被哥本哈根市伦理委员会批准,符合赫尔辛基宣言的原则。
美国的PENN CATH研究,是在1998年7月到2003年3月之间在宾夕法尼亚大学医学中心经历了心导管插入术的连续患者组中进行的生物化学和遗传因子与冠状动脉粥样硬化的关联性的横剖面研究。2型糖尿病被定义为在大于40岁的对象中,具有空腹血糖≥126mg/dl、餐后2小时血糖≥200mg/dl、使用口服降糖药剂或胰岛素和口服降糖药剂的历史。宾夕法尼亚大学组织审查委员会批准了研究计划,所有的对象给出了正式的书面同意书。种族划分通过自己报告来确定。该组中总共出现了468例高加索人2型糖尿病病例。此外,从同样的研究中随机抽取了1024个未患病的(相对于2型糖尿病来说)高加索人对照。
用于基因分型的DNA,是使用GenomiPhi扩增试剂盒(Amersham),对从丹麦和美国2型糖尿病患者和对照的外周血分离的DNA进行全基因组扩增的产物。
基因分型
使用来自Illumina的Infinium HumanHap300SNP芯片,对1399个冰岛糖尿病患者进行了基因组范围的扫描,以在单一芯片(Illumina,San Diego,CA,USA)上分析大约317,000个单核苷酸多态性(SNPs)。在其它病例-对照组中进行的SNP基因分型的重复使用Centaurus平台(Nanogen)来进行。
关联性分析的统计学方法
对于单标记物与2型糖尿病的关联来说,我们使用了或然性比率检验以计算每个等位基因的双面p-值。我们根据乘法模型假设计算了相对风险(RR)和群体归因风险(PAR)(C.T.FaIk,P.Rubinstein,AnnHum Genet 51(Pt 3),227(1987);J.D.Terwilliger,J.Ott,Hum Hered 42,337(1992))。对于CEPH高加索人HapMap数据来说,我们使用了D′(R.C.Lewontin,Genetics 50,757(1964))和R2(W.G.Hill,A.Robertson,Genetics 60,615(Nov,1968))的标准定义来计算成对SNPs之间的LD。当对所有的SNP组合进行作图以阐明特定区域中的LD结构时,我们将D′作图在左上角,p-值作图在右下角。在我们给出的LD图中,标记物被等距离作图,而不是按照它们的物理位置作图。
结果
基因组范围的关联性研究
使用Illumina 330K芯片,我们对1399个冰岛2型糖尿病患者和5275个群体对照个体进行了成功的基因分型。使用LD区块中的单一SNPs、双标记物单倍型和延长的单倍型,进行了关联性分析。在根据亲缘性校正了p-值之后,我们在21个位点上(即基因组中的遗传易感性位置)鉴定了49个单标记物和双标记物单倍型,它们的p-值小于5x10-5(表1)。此外,通过同样的标准选择了位于8个其它位点上的10个延长的单倍型(表2)。在患者组中,700个个体是不肥胖的(BMI<30),它们的关联性被单独测试。在根据亲缘性校正了p-值后,在20个位点上鉴定了36个单标记物和双标记物单倍型,它们的p-值小于5x10-5(表3)。这些位点中有3个在对整个组进行分析时也鉴定到了。此外,通过同样的标准选择了位于4个其它位点上的6个延长的单倍型(表4)。531位患者的肥胖组(BMI>30)的关联性也被单独分析。在根据亲缘性校正了p-值后,在16个位点上鉴定了38个单标记物和双标记物单倍型,它们的p-值小于5x10-5(表5)。这些位点中有1个在对整个组进行分析时也鉴定到了,但是使用该标准,在不肥胖组和肥胖组之间没有发现重叠。此外,位于7个其它位点上的10个延长的单倍型在肥胖型糖尿病的关联性分析中具有小于5x10-5的p-值(表6)。
因此,表1-6中显示的单标记物关联性和双标记物和延长的单倍型关联性,为2型糖尿病的多个易感性变体显示了证据。应该指出,对于本文显示的单标记物SNP分析来说,易感性变体可以表现为增加的风险,其中与对照相比,一个等位基因在患者组中出现过多。或者,易感性变体可以表现为正在讨论的SNP的另一个等位基因,对于该等位基因来说,与对照组相比,预计在患者中将出现较少。这是对含有两个等位基因的遗传元件进行关联性分析的自然结果。对于多标记物单倍型或含有一个以上标记物的多态性标记物来说,可以观察到与一个(或多个)风险等位基因或单倍型的风险关联性。依赖于正在讨论的遗传区域中的遗传组成和单倍型结构,也可以观察到与一个(或多个)保护性变体或单倍型相关联(RR值小于整体)的形式的保护性变体。
表1、与2型糖尿病相关的单标记物和双标记物单倍型。相关联的等位基因显示在每个标记物的前面(等位基因编码:A=1,C=2,G=3,T=4)
Chr Pos Punadj Padj Rrisk Aff.frq Ctrl.frq 单倍型
chr1 151511890 4.01E-06 4.49E-05 1.223 0.407 0.360 3 rs3738028
chr2 40560735 2.41E-06 3.06E-05 1.225 0.593 0.543 1 rs13414307
chr2 40560735 4.59E-07 8.27E-06 1.243 0.571 0.517 1 rs13414307 3 rs1990609
chr2 54969849 5.53E-08 1.56E-06 1.287 0.335 0.281 3 rs930493 4 rs10173697
chr2 54977961 3.12E-06 3.75E-05 1.224 0.553 0.503 4 rs10173697
chr3 89323970 2.60E-06 3.25E-05 1.380 0.904 0.872 4 rs12486049
chr6 6965113 1.00E-06 1.53E-05 1.705 0.072 0.044 1 rs490213 3 rs814174
chr6 31556294 3.22E-06 3.78E-05 1.232 0.372 0.325 2 rs2516424
chr6 31556294 1.93E-06 2.57E-05 1.240 0.368 0.320 2 rs2516424 2 rs4947324
chr6 132422361 3.10E-06 3.74E-05 1.262 0.278 0.234 3 rs9483377 2 rs997807
chr6 132422361 3.97E-06 4.54E-05 1.252 0.276 0.233 3 rs9483377 3 rs7745875
chr6 132422361 7.98E-07 1.25E-05 1.249 0.356 0.307 3 rs9483377
chr6 150460378 5.01E-07 8.86E-06 1.293 0.794 0.749 1 rs11155700
chr6 150461077 5.15E-07 9.05E-06 1.292 0.794 0.749 2 rs12213837
chr6 164474219 3.07E-06 3.63E-05 0.813 0.479 0.531 4 rs206732 2 rs933251
chr7 87951463 4.36E-06 4.89E-05 1.273 0.753 0.705 1 rs2192319
chr8 124196776 1.21E-06 1.78E-05 1.253 0.721 0.673 3 rs952656
chr8 124202699 5.97E-07 9.96E-06 0.722 0.108 0.143 4 rs13252935 3 rs7824293
chr9 90164936 2.03E-06 2.62E-05 1.304 0.192 0.154 1 rs10993008
chr9 95493692 2.38E-06 3.03E-05 1.253 0.309 0.263 3 rs10990568 3 rs4743148
chr9 95510129 5.85E-07 9.80E-06 1.252 0.365 0.315 3 rs4743148
chr10 53058229 1.39E-06 1.98E-05 1.240 0.377 0.328 4 rs7915186 4 rs3829170
chr10 53063104 1.37E-06 1.96E-05 1.239 0.386 0.336 4 rs3829170 3 rs7922112
chr10 94301795 2.54E-08 8.44E-07 1.276 0.614 0.555 3 rs2421943
chr10 94301795 2.11E-09 1.19E-07 1.297 0.585 0.521 3 rs2421943 2 rs7917359
chr10 94304784 1.49E-07 3.32E-06 0.797 0.443 0.499 3 rs7908111 3 rs2497304
chr10 94309972 6.60E-09 2.85E-07 0.779 0.455 0.517 3 rs1999763 4 rs10882091
chr10 94309972 6.60E-09 2.85E-07 0.779 0.455 0.517 3 rs1999763 3 ts6583830
chr10 94337810 1.36E-06 1.91E-05 1.228 0.518 0.467 3 rs6583826
chr10 94337810 7.18E-08 1.91E-06 1.262 0.449 0.393 3 rs6583826 2rs 10882091
chr10 94364357 7.76E-08 2.04E-06 1.259 0.466 0.410 2 rs10882091 3 rs7923837
chr10 94364357 9.33E-08 2.30E-06 1.256 0.472 0.415 2 rs10882091
chr10 94372930 9.81E-08 2.40E-06 1.256 0.472 0.415 4 rs7914814
chr10 94388098 9.33E-08 2.30E-06 1.256 0.472 0.415 1 rs6583830
chr10 94442410 8.41E-08 2.17E-06 1.256 0.527 0.470 1 rs2275729 3 rs1111875
chr10 94482696 7.56E-08 1.95E-06 1.258 0.542 0.485 1 rs2497304
chr10 94485733 1.64E-06 2.21E-05 1.225 0.526 0.475 1 rs947591
chr12 33373479 3.87E-06 4.37E-05 1.391 0.110 0.082 4 rs1905421
chr15 98156854 3.80E-06 4.30E-05 0.815 0.469 0.521 1 rs9920347 3 rs11635811
chr16 22705353 2.93E-06 3.57E-05 1.264 0.781 0.738 4 rs724466
chr16 72066252 4.23E-06 4.68E-05 0.625 0.038 0.059 2 rs1862773 4 rs825842
chr16 72086481 5.86E-07 9.82E-06 0.612 0.043 0.069 4 rs2432543 3 rs4887826
chr17 66072384 7.34E-07 1.20E-05 1.236 0.564 0.511 3 rs17763769 1 rs1860316
chr17 66117911 1.18E-07 2.77E-06 0.781 0.282 0.335 3 rs1860316 2 rs17763811
chr17 66117911 6.79E-08 1.83E-06 1.281 0.707 0.653 1 rs1860316
chr17 66132788 1.80E-06 2.43E-05 1.226 0.563 0.513 2 rs1981647
chr17 66149102 1.39E-06 1.99E-05 1.239 0.665 0.615 4 rs1843622
chr17 66159416 7.32E-07 1.19E-05 1.266 0.744 0.696 1 rs2191113
chr20 36391335 2.09E-07 4.45E-06 1.250 0.550 0.495 3 rs4592915 2 rs2232580
表3、在不肥胖患者中与2型糖尿病相关联的单标记物和双标记物单倍型
*相关联的等位基因显示在每个标记物的前面(等位基因编码:A=1,C=2,G=3,T=4)。
表5、在肥胖患者中与2型糖尿病相关联的单标记物和双标记物单倍型
*相关联的等位基因显示在每个标记物的前面(等位基因编码:A=1,C=2,G=3,T=4)。
染色体6p22.3位点
对于不肥胖的糖尿病患者来说,鉴定到的最显著的关联性信号之一是作图于染色体6p22.3的两个单标记物(rs1569699和rs7756992)和两个双标记物单倍型(表3)。这些标记物位于标记物rs4429936和rs69084252之间的20634996-20836710位碱基(NCBI Build 35)的LD区块内(SEQ ID NO:1;图1)。该LD区块含有类CDK5调节亚基相关蛋白1的成员1(CDKAL1)基因的5’末端,包括外显子1-5(NM_017774)。CDKAL1蛋白具有催化活性以及铁离子结合活性,但是体内功能未知。它被广泛表达,包括表达在胰腺中。
为了证实rs1569699和rs7756992与2型糖尿病的关联性,在丹麦2型糖尿病病例-对照组以及来自PENN CATH研究的美国高加索人群2型糖尿病组中,对两个标记物进行了基因分型(表7)。结果显示出,在丹麦组中两个标记物与2型糖尿病显著关联,并且它在US UPenn.组中也赋予了相似的风险,尽管结果没有达到统计学显著性。当将两个重复组合并起来时,结果是显著的,风险为大约1.2。当将所有组合并起来时,比较了将近3000个2型糖尿病患者(不考虑BMI)和超过8000个对照的组,每个标记物的风险超过1.2。这些结果在基因组范围内是显著的。
表7、rs1569699和rs7756992与2型糖尿病的关联性
这些结果显示出在2型糖尿病中,与6号染色体上标记物rs4429936和rs6908425之间的20634996-20836710bp区域(Build 34)的显著关联性。在所有三个组中,相对风险(RR)的值是相当的;在UPenn组中水平为0.05的显著相关性的缺乏,主要是由于缺少与其它组相比较的权,尽管与冰岛组相比,该组中的RR值略低(对于rs1569699来说RR为1.185比1.224)。此外,在冰岛组中,不肥胖2型糖尿病患者的RR值甚至更高(对于rs1569699来说RR=1.33)。
染色体10q23.33位点
在染色体10q23.33区域中发现了与2型糖尿病相关联的7个单标记物和7个双标记物单倍型(表1)。当肥胖患者被单独分析时,大多数这些标记物也与糖尿病相关,具有升高的RR值(表5)。这些标记物位于94192885和94490091位置(NCBI Build 35)之间的一个LD区块中,对应于标记物rs2798253和rs11187152之间的基因组区段(图2)。该LD区块含有三个基因:胰岛素降解酶(IDE)(NM_004969)、驱动蛋白家族11号成员(KIF11)(NM_004523)和造血表达同源异形盒(HHEX)(NM_002729)。
IDE可能属于负责细胞内肽信号转导的蛋白酶家族。尽管它在胰岛素的细胞加工中的作用还没有被确定,但胰岛素降解酶被认为参与了胰岛素反应的终止(Fakhrai-Rad等,Human Molecular Genetics9:2149-2158,2000)。糖尿病GK大鼠的遗传分析已经发现,在GK等位基因中,在IDE基因中的两个氨基酸取代(H18R和A890V),使转染的细胞中胰岛素降解活性降低了31%。但是,当对H18R和A890V变体进行分别研究时,没有观察到影响,表明两个变体对胰岛素降解具有协同效应。在细胞裂解液中没有观察到对胰岛素降解的影响,表明影响可能与胰岛素的受体介导的内化作用相偶联。带有IDE GK等位基因的同类系大鼠显示出了餐后高血糖,脂肪细胞中的低脂肪生成,钝化的胰岛素刺激的葡萄糖跨膜摄取,以及在分离的肌肉中降低的胰岛素降解。对其它大鼠株系的分析证实了功能障碍的IDE等位基因是GK大鼠所独有的。作者得出结论,在GK大鼠中,IDE在糖尿病表现型中扮演了重要角色。IDE作为2型糖尿病的候选基因在人类中的研究得到了不一致的结果。最近,两项大的研究,通过突变筛选和使用标签SNPs在基因上进行的单倍型分析,分析了IDE与2型糖尿病的关联性(Groves等,Diabetes 52:1300-1305,2003;Florez等,Diabetes55:128-135,2006)。两项研究均得出结论,常见的IDE变体不可能赋予对2型糖尿病的显著的风险。但是,这些研究没有包含在图2中定义的全部LD区块,至少某些在我们的研究中被鉴定为与2型糖尿病相关的标记物,位于上述那些研究所分析的区域之外。基于这里报告的结果,与IDE连锁不平衡的标记物与2型糖尿病有关联,为IDE在2型糖尿病的病因学中的作用提供了遗传证据。
KIF11编码属于驱动蛋白(kinesin)类蛋白家族的马达蛋白。该蛋白家族的成员已知参与了各种不同种类的纺锤体动力学。该基因产物的功能包括细胞有丝分裂过程中的染色体定位、中心体分离和双极纺锤体的建立。该基因不是糖尿病的好的功能候选物,但是由于其位于相关的LD区块中,不得不被当做位置候选物。
HHEX编码了转录因子的同源异形盒家族的成员,它们中的许多参与了发育过程。在特定造血株系中的表达,表明该蛋白可能在造血分化中发挥作用。HHEX对于胰腺发育是基本的;在HHEX阴性小鼠胚胎中,腹侧胰腺的特化完全失败(Bort等,Development 131,797-806,2004)。其它参与胰腺发育的转录因子包括MODY基因以及其它已被暗示与晚期发作的糖尿病有关的因子。HHEX也是用于心脏诱导的Wnt拮抗剂的基本效应子(Foley和Mercola,GENES & DEVELOPMENT19:387-396,2005)。这将HHEX置于与最近建立的2型糖尿病基因TCF7L2同样的途径中,这些数据合在一起,使HHEX成为2型糖尿病的功能和位置候选物。
为了证实rs2497304、rs947591、rs10882091和rs7914814与2型糖尿病的关联性,在丹麦2型糖尿病病例-对照组中以及来自PENNCATH研究的美国高加索人2型糖尿病组中进行了标记物的基因分型(表8)。结果显示在单独的任何一个组中,关联性没有得到重复。但是,当将两个组合并时,rs947591的关联性达到了显著性为0.05的水平,在合并的组中风险为1.1。当所有的组合并起来时,对于rs947591标记物来说风险是1.15。
这些结果表明,10号染色体上的含有IDE和HHEX的LD区块中的变体是2型糖尿病的易感性变体。
表8、在冰岛、丹麦和美国组中10号染色体上的标记物与2型糖尿病的关联性分析
染色体17q24.3位点
在不肥胖患者中,在染色体17q24.3区域中发现了5个单标记物和两个双标记物单倍型与2型糖尿病有关联(表3)。这些标记物中的某些显示出了表3中报告的最强的关联性,在对所有糖尿病患者进行的分析中也观察到了与该区域的关联性(表1)。这些标记物位于17号染色体上66037656和66163076位置(NCBI Build 35)之间的两个邻近的LD区块中,在标记物rs11077501和rs4793497之间(图3)。在校正了在单标记物关联性分析中进行的测试的数量之后,关联性是显著的;即在全基因组水平上,关联性是显著的。在这些LD区块中没有已知的基因。但是,有可能该区域中的变体影响了邻近区域中的基因,包括KCNJ2和KCNJ16。或者,这些变体可能影响了这些LD区块中的未知基因。
表9、位于CDKAL1基因中的SNPs(位于6号染色体上NCBIBuild 35和NCBI Build 36中的20,642,736和21,340,611位置之间)
表10、6号染色体上NCBI Build 35和NCBI Build 36中20,634,996和20,836,710bp位置之间的LD区块C06(SEQ ID NO:1)中的SNPs。
表11、10号染色体上NCBI Build 35和NCBI Build 36中94,192,885和94,490,091bp位置之间的LD区块C10(SEQ ID NO:2)中的SNPs。
表12、17号染色体上NCBI Build 35和NCBI Build 36中66,037,656和66,163,076bp位置之间的LD区块C17中的SNPs。
表13、本文提供的重要序列列表
SEQ ID NO | 名称 |
1 | LD block C06 |
2 | LD block C10 |
3 | LD block C17 |
4 | rs10882091 |
5 | rs1111875 |
6 | rs1569699 |
7 | rs17763769 |
8 | rs17763811 |
9 | rs1843622 |
10 | rs1860316 |
11 | rs1981647 |
12 | rs1999763 |
13 | rs2191113 |
14 | rs2275729 |
15 | rs2421943 |
16 | rs2497304 |
17 | rs3829170 |
18 | rs4712527 |
19 | rs6583826 |
20 | rs6583830 |
21 | rs7756992 |
22 | rs7758851 |
SEQ ID NO | 名称 |
23 | rs7908111 |
24 | rs7914814 |
25 | rs7915186 |
26 | rs7917359 |
27 | rs7922112 |
28 | rs7923837 |
29 | rs9295478 |
30 | rs947591 |
31 | rs9890889 |
32 | rs7752906 |
33 | rs9350271 |
34 | rs9356744 |
35 | rs9368222 |
36 | rs10440833 |
37 | rs6931514 |
38 | rs2009802 |
39 | rs17718938 |
40 | rs17223216 |
41 | rs2109050 |
42 | rs1962801 |
43 | rs7086285 |
44 | rs17234378 |
实施例2
CDKAL1基因中的变体影响了胰岛素反应和2型糖尿病风险
最近,我们描述了TCF7L2中与T2D相关的变体(Grant,S.F.等,Nat Genet 38,320-3(2006);Helgason,A.等,Nat Genet(2007))。下面,我们描述使用Illumina Hap300芯片,对冰岛T2D患者进行的基因组范围的关联性研究。我们在1399个T2D患者和5275个对照的样品中分别测试了313,179个SNPs与T2D的关联性。我们对使用一组没有包含在Hap300芯片中、但是在HapMap项目中被分型的(Pe′er,I.等,Nat Genet 38,663-7(2006))SNPs鉴定为有效替代物(r2>0.8)的339,846个双标记物单倍型,进行了进一步测试。除了分析整个T2D患者组之外,我们还分别测试了700个不肥胖的T2D患者和531个肥胖的T2D患者的关联性。总的来说,总共进行了1,959,075个(653,025个变体x3种表现型)测试。结果针对个体之间的亲缘性和可能的群体分层现象通过基因组控制进行了调整(Devlin,B.& Roeder,K.Biometrics 55,997-1004(1999))(参见方法)。具体来说,对于所有不肥胖的和肥胖的T2D病例来说,将(未调整的)卡方(chi-square)统计值分别除以1.287、1.204和1.184。以前在TCF7L2基因中鉴定到的SNP rs7903146在所有T2D患者中给出了最显著的结果,OR=1.38,P=1.82x10-10。尽管在根据执行的测试的数量进行调整之后,没有其它的SNP或单倍型是显著的,但观察到了比单纯根据几率所预计到的更多的边界线上的显著信号(图4)。因此,我们决定进一步研究上述信号。
方法
冰岛人研究群体
冰岛T2D组以前已经描述过(Reynisdottir,I.等,Am J Hum Genet73,323-35(2003))。为了进行本基因组范围的关联性研究,征集了总共1500个T2D患者,使用Infinium II分析方法和Sentrix HumanHap300BeadChip进行研究(Illumina,San Diego,CA,USA)。其中,按照我们的质量控制标准(参见补充方法),对1399人进行了成功的基因分型并用于本病例-对照分析中;531个被基因分型的病例是肥胖的(BMI≥30)。用于该研究的对照组由599个从冰岛谱系数据库中随机选择的对照,和4676个来自deCODE的正在进行的其它基因组范围的关联性研究的个体组成。研究得到了冰岛数据保护委员会和冰岛国家生物伦理委员会的批准。从所有病例和对照获得了正式的书面同意书。
其它研究群体
282个病例和629个对照的丹麦女性研究组、在这里称为丹麦A组,选自在丹麦进行的远景流行病学风险因子(PERF)研究(Tanko,L.B.,等,Bone 32,8-14(2003))。这是一组参加了在临床和基础研究中心进行的各种筛选性安慰剂对照临床试验和流行病学研究的绝经后女性。在2000-2001年对5847位女性进行的跟踪检查中,在个人会谈中使用预制的调查问卷收集了医学史信息,包括I型和II型糖尿病、家族史和目前或以前的药物的长期使用。如果对象被诊断为I型或II型糖尿病,也将收集诊断或治疗的时间。研究得到了哥本哈根市伦理委员会的批准,符合赫尔辛基宣言的原则。
1359个T2D病例和4858个具有正常葡萄糖耐受的对照个体的第二个丹麦研究群体,来自于哥本哈根的Steno糖尿病中心,以及生活在大哥本哈根地区、并在预防和卫生研究中心28取样的30-60岁个体的Inter99基于群体的样品(Jorgensen,T.等,Eur J Cardiovasc PrevRehabil 10,377-86(2003))。该数据组在本文中被称为丹麦B组。糖尿病和糖尿病前期范畴的诊断按照1999年世界卫生组织(WHO)的标准。对于Inter99研究中的参加者按照描述进行了口服葡萄糖耐受性测试(Jorgensen,T.等,Eur J Cardiovasc Prev Rehabil 10,377-86(2003))。在参加之前,从所有对象获得了正式的书面同意书。研究被哥本哈根市伦理委员会批准,符合赫尔辛基宣言的原则。
费城研究群体由468个T2D病例和1024个对照个体组成。研究群体选自PENN CATH研究,这是在宾夕法尼亚大学医学中心经历了心导管插入术的连续个体的研究群体中进行的生物化学和遗传因子与冠状动脉粥样硬化的关联性的横剖面研究。T2D被定义为在大于40岁的对象中,具有空腹血糖≥126mg/dl-1、餐后2小时血糖≥200mg/dl-1、使用口服降糖药剂或使用胰岛素和口服降糖药剂的历史。宾夕法尼亚大学组织审查委员会批准了研究计划,所有的对象给出了正式的书面同意书。所有的病例和对照是欧洲人血统。种族划分通过自己报告来确定。
荷兰Breda研究群体由370个T2D病例和916个对照个体组成。病例是在1998-1999年与Breda糖尿病服务部(Diabetes Service Breda)和80位全科医生合作从Breda周围的地区征集的。所有的患者按照WHO的标准进行诊断(血浆葡萄糖水平>11.1mmol/l或空腹血浆葡萄糖水平≥7.0mmol/l),并经历了对他们的糖尿病进行的定期的三个月间隔的临床和实验室评估。Utrecht大学医学中心的医学伦理委员会批准了研究方案。所有的先证者填写了书面同意书和关于临床数据的调查问卷,包括他们的与糖尿病相关的药物治疗,目前以及20岁时的身高和体重。对照是荷兰血液库捐献者,平均年龄为48岁。
苏格兰研究群体由来自Tayside糖尿病检查和研究项目(DARTS)的子研究项目Wellcome Trust UK T2D病例-对照收集库(Go-DARTS2)(Morris,A.D.等,BMJ 315,524-8(1997))的2型糖尿病病例和无糖尿病的对照。所有的T2D患者是在一级和二级糖尿病护理诊所征集、或从一级护理登记者中邀请参加的的医生诊断的T2D病例,还没有进行GAD抗体或MODY基因突变表征。对照由一级护理医生或对象工作场所的职业卫生部门邀请参加。对照以前没有被诊断为糖尿病,但是对照的葡萄糖耐受状态是未知的。在该正在进行的研究中的所有个体是在2004年10月到2006年7月之间在Tayside征集的。本研究得到了Tayside医学伦理委员会的批准,从所有对象获得了书面同意书。
香港研究群体中的所有对象都是居住在香港的中国南方汉族血统。病例由1500个从威尔士王子医院糖尿病登记处选择的患有T2D的个体组成。其中,682个患者患有年轻时发病的糖尿病(诊断时年龄≤40岁),并具有阳性家族史。另外818个病例从同一个登记处随机选择。对照组由1000个具有正常葡萄糖耐受性的个体(空腹血浆葡萄糖<6.1mmol/l)组成。其中,617人从参加基于社区的心血管风险筛查项目的普通人群以及医院工作人员中召集。此外,383人从青年人心血管风险筛查项目召集。从每个参加的对象获得了书面同意书。本研究得到了香港中文大学临床研究伦理学委员会的批准。
非洲人研究群体来自于非洲裔美国人糖尿病研究,它最初被设计为患病的兄弟姐妹对的研究,加入了可用的配偶作为对照。它已经被扩展到包含了患病对的其它家庭成员以及群体对照。在该报告中登记的家庭的召集策略和资格标准以前已经描述过(Rotimi,CN.等,AnnEpidemiol 11,51-8(2001))。西非病例-对照系列由来自尼日利亚的约鲁巴人(233个患病个体,432个对照)和伊博人(237个患病个体,276个对照)组,以及来自加纳的Akan(257个患病个体,248个对照)、埃维人(22个患病个体,30个对照)和Gaa-Adangbe(123个患病个体,141个对照)组组成。
除了苏格兰Go-DARTS研究群体之外,在所有重复研究群体中用于基因分型的DNA是从外周血分离的DNA的全基因组扩增产物(GenomiPhi扩增试剂盒,Amersham)。
统计学分析
Illumina全基因组基因分型。使用含有来自国际HapMap项目第一期的317,503个单倍型标签SNPs的Infinium HumanHap300SNP芯片(Illumina,SanDiego,CA,USA),对所有冰岛的病例和对照样品进行了分析。在芯片上分析的SNPs中,4,324个SNPs被排除,因为(a)在病例和对照中产率低于95%;(b)在群体中次要等位基因的频率低于1%;或(c)在对照中显示出与Hardy-Weinberg平衡的显著的偏离(P-值<0.001)。任何调用比率低于98%的样品被排除在分析之外。因此,在本文中显示的最终分析使用了313,179个SNPs。
单SNP基因分型。除了苏格兰Go-DARTS群体之外,所有研究的群体的单SNP基因分型在冰岛雷克雅未克的deCODE Genetics,通过Centaurus(Nanogen)平台来进行(Kutyavin,I.V.等,Nucleic Acids Res34,e128(2006))。每个Centaurus SNP分析的质量,通过对CEU和/或YRI HapMap样品中的每个分析进行基因分型,并将结果与HapMap数据相比较来评估。具有大于1.5%错配率的分析被弃用,对于已知处于连锁不平衡的标记物使用了连锁不平衡(LD)测试。苏格兰群体的单SNP基因分型在苏格兰Dundee的Ninewells医院和医学院的生物医学研究中心进行,使用了方法。
关联性分析。对于关联性分析来说,我们使用了标准的或然性比率统计学,在NEMO软件中运行(Gretarsdottir,S.等,Nat Genet 35,131-8(2003)),根据风险的乘法模型假设,即一个人携带的两个等位基因的风险相乘,计算了每个个体等位基因的双面p-值和等位基因特异性OR。对于标记物来说显示了等位基因频率而不是携带者频率,在根据对象的亲缘性进行调整之后,给出了p-值。当估算基因型特异性OR(表19)时,根据HWE假设对群体中的基因型频率进行估算。
总的来说,通过最大或然性估算等位基因/单倍型频率,并使用通用的或然性比率检验(Rice,J.A.《数学统计学和数据分析》,Mathematical Statistics and Data Analysis,(Wadsworth Inc.,Belmont,CA,1995))来进行病例和对照之间的差异的检验。该方法特别适用于目标标记物的某些基因型丢失的情况下,这时另一个与目标标记物强烈连锁不平衡的标记物的基因型被用于提供一些部分信息。这被使用在表17中显示的关联性测试中,以确保可以使用同样数量的个体进行高度相关的标记物的比较。为了处理阶段的不确定性和丢失的基因型,对于观察到的数据直接计算了最大或然性估算值、或然性比率和p-值,因此,由于阶段的不确定性和丢失的基因型导致的信息的丢失被或然性比率自动捕获。
使用Mantel-Haenszel模型(Mantel,N.& Haenszel,W.J NatlCancer Inst 22,719-48(1959))将多个病例-对照组的结果合并,其中组被允许具有不同的等位基因群体频率,但是基因型被假定具有共同的相对风险。
对对象的亲缘性的校正和基因组对照。在冰岛患者和对照组中的某些个体是彼此相关的,导致卡方测试统计学数据的平均值大于1,中值大于0.6752。我们通过计算653,025各卡方统计学数据的平均值,估算了膨胀因子,这是用于对亲缘性和可能的群体分层现象进行调整的基因组对照4的方法。对于所有、不肥胖的和肥胖的T2D病例来说,膨胀因子分别被估算为1.287、1.204和1.184。显示的结果是基于将每个卡方统计数据除以相应的膨胀因子而对它们进行的调整。
定量分析。从对丹麦Inter99研究的个体进行的口服葡萄糖耐受测试获得的数据,被用于将胰岛素分泌计算为校正的胰岛素反应(CIR),使用了下面的公式:(100x30分钟时的胰岛素)/[30分钟时的葡萄糖×(30分钟时的葡萄糖-3.89mmol)]。胰岛素的敏感性被估算为按照稳态模型评估(HOMA)的胰岛素抗性的倒数:22.5/[空腹胰岛素x空腹葡萄糖](Matthews,D.R.等,Diabetologia 28,412-9(1985))。CIR(HOMA)与基因型状态之间的关联性使用多次回归来测试,其中对数转换的CIR(HOMA)被取作反应变量,解释变量是个体携带者的风险等位基因的拷贝数(累加模型),或风险等位基因的纯合携带者的指示符变量(回归模型)。通过包含适当的项作为解释变量,根据性别、年龄和患病状态进行了调整。为了进行比较,胰岛素的分泌也被计算为(30分钟时的胰岛素-0分钟时的胰岛素)/(30分钟时的葡萄糖-0分钟时的葡萄糖),产生了可比较的结果。
细胞株。INS1细胞由Hoffmann-LaRoche提供。它们生长在添加有10%胎牛血清(Invitrogen)、50μg/ml青霉素-链霉素(Invitrogen)、50μM 2-巯基乙醇(SIGMA)、1mM MEM丙酮酸钠(Invitrogen)和10mM Hepes缓冲液(Invitrogen)的RPMI1640(Invitrogen)中。每周,通过在1X Hanks平衡盐溶液(Invitrogen)中清洗两次然后用胰蛋白酶消化(胰蛋白酶-EDTA;Invitrogen),将它们按1∶2分开。
RNA的制备和cDNA扩增。将INS1细胞在含有10mM葡萄糖的正常生长培养基中温育48小时。收获时含有2x107个细胞,被用于制备总RNA。使用RNeasy Midi试剂盒(Quiagen)提取RNA。使用高能cDNA Archive试剂盒(Applied Biosystems)制备cDNA。使用两个不同的引物对扩增CDKAL1 cDNA,它们位于外显子2和8之间(正向:5′-GGGGCTGCTCACATAATAATTCA-3′;反向5′-TGTGCCAATGTCTCTGCCATA-3′)和外显子7和13之间(正向:5′-ACCTGGCCAGCTATCCCATT-3′;反向:5′-CCATTTTTCCCATGAATGCAG-3′)。来自β-肌动蛋白的引物用作阳性对照(正向:5′-ATCTGGCACCACACCTCCTACAATGAGCTGC-3′;反向:5′-CGTCATACTCCTGCTTGCTGATCCACATCTGC-3′)。
结果和讨论
对于每个被测试的表现型来说,我们选择了所有P<0.00005的单SNPs和双标记物单倍型,用于在丹麦组(丹麦B组)的病例-对照样品中进行重复。在消除了多余的标记物后,取总共46个SNPs进行进一步的重复尝试(表14)。此外,我们包含了在Illumina Hap300芯片上存在的5个最显著的非同义的SNPs。在那51个SNPs中,在1110个丹麦T2D病例和2272个对照中对47个进行了成功的基因分型。在丹麦组中,SNPs rs7756992和rs13266634引人注目并明显被重复出来,在丹麦组的所有T2D患者中分别具有P=0.00013和OR=1.24以及P=0.0012和OR=1.20(表15)。这与在最初的冰岛研究中分别获得的P=0.00021和OR=1.23以及P=0.000061和OR=1.19相当。所有其它被基因分型的SNPs在丹麦组中P>0.01,没有被进一步追踪。第一个SNP,rs7756992,位于6p22.3上类CDK5调节亚基相关蛋白1蛋白成员1(CDKAL1)基因的内含子5中。它位于201.7kb的大的连锁不平衡区块中,该区块包含CDKAL1基因的外显子1-5以及最小启动子区,但是没有其它已知的基因(图5)。第二个SNP,rs13266634,是一个非同义的SNP,导致8q24上溶质载体蛋白家族30(锌转运蛋白)成员8(SLC30A8)基因的最后一个外显子中325位的精氨酸变为色氨酸。SLC30A8的基因产物是胰腺特异性的,它被表达在β细胞中,在那里其促进锌从细胞质积累到细胞内囊泡中(Chimienti,F.等,Diabetes 53,2330-7(2004))。最近,在法国T2D病例和对照的全基因组关联性研究中,发现了8q24上的rs13266634的风险等位基因赋予了对T2D的风险(Sladek,R.等,Nature 445,881-5(2007))。在该研究中其它显著相关的SNPs中,我们也在起始冰岛样品中重复了与HHEX基因附近的两个SNPs的关联性(表16)。但是,我们没有重复出与该研究中也描述了的LOC387761和EXT2基因中的标记物的所报告的关联性
我们在来自丹麦(丹麦A组)、苏格兰、荷兰和美国费城的欧洲血统的4个其它的T2D病例-对照组、以及来自香港和西非的病例-对照组中,对SNPs rs7756992和rs13266634进行了基因分型。此外,通过增加被基因分型的对照的数量,丹麦B研究组的大小被极大地扩展了。rs7756992的G等位基因的关联性在苏格兰(OR=1.11;P=0.0042)和香港(OR=1.25;P=0.00018)病例-对照组中被明显重复出来(表17)。在其它研究组中的关联性在单独情况下不显著,但是都具有同样的方向。从所有8个病例-对照组的合并观察到的关联性给出了OR为1.15,相应的P值为9.0x10-12(表17)。假设在最初的基因组扫描中进行了大约2百万个测试,进行Bonferonni调整后该关联性仍然是高度显著的(Padj=1.8x10-5)(Skol,A.D.等,Nat Genet 38,209-13(2006))。通过对与HapMap CEPH(CEU)数据组中的原始信号相关的其它标记物进行基因分型,以精炼观察到的与rs7756992的关联性的尝试,没有产生更显著的结果(表18)。正如预计的那样,对西非群体观察到的连锁不平衡与对冰岛和香港组观察到的相比较少得多(表19)。还需要进一步的工作,以确定在西非组中是否能够鉴定到具有比rs7756992观察到的更高的OR的相关变体。同样,对于非同义的SNPrs13266634的等位基因C来说,在6个其它组中的三个中(来自苏格兰、费城和香港),与T2D的关联性被显著重复出来(表17)。尽管丹麦B组的OR随着样品大小的增大而降低,并且估算的效果与丹麦A组的方向相反(仅仅轻微的,不显著),但来自所有研究组的合并的结果产生了全基因组范围的显著的P值为2.5x10-11和OR为1.16(表17)。
在冰岛研究中,与rs7756992的关联性在不肥胖的T2D患者中(OR=1.37;P=9.0x10-6)比在所有患者的组中(OR=1.23;P=0.00021)更显著(表14和表17)。在研究的其它群体中,对于该变体来说也观察到了在不肥胖的比在肥胖的T2D患者中更高的OR。对于欧洲来源的合并的群体来说,不肥胖T2D患者的OR是1.19,P=7.29x10-9,与此相比肥胖组的OR=1.12,P=0.00017。在香港组不肥胖T2D组(OR=1.36,P=7.48x10-6)中观察到甚至更强的效应,与此相比肥胖组的OR=1.13,P=0.094,其中肥胖被定义为BMI≥25。当所有组的结果被合并时,相对于对照,对于不肥胖的和肥胖的T2D患者组,分别获得了OR=1.19,P=1.93x10-11以及OR=1.13,P=2.68x10-5。这些结果表明,该变体不通过增加的BMI赋予T2D的增加的风险。
对于两个位点中的每个估算了基因型比值比(表20)。基于合并的高加索人研究群体的结果,rs7756992明显偏离了乘法模型,杂合子的OR为1.09,与此相比纯合子的OR为1.45,这支持了遗传的接近隐性的模型。在香港组样品中观察到了同样的、尽管不太显著的趋势,杂合子的OR为1.13,纯合子的OR为1.55。相反,对于rs13266634来说,基因型相对风险的乘法模型提供了足够的拟合。
CDKAL1基因产物的功能是未知的。但是,正如基因的名字所暗示的,蛋白产物与另一种蛋白CDK5调节亚基相关蛋白1(CDK5RAP1)类似。CDK5RAP1在神经元组织中表达,在那里它通过结合CDK5调节亚基p35抑制周期蛋白依赖性激酶5(CDK5)的活性(Ching,Y.P.,等,J Biol Chem 277,15237-40(2002))。在胰腺β细胞中,已经显示出在糖毒性条件下CDK5在β细胞功能的丧失中扮演了重要角色(Wei,F.Y.等,Nat Med 11,1104-8(2005))。此外,对CDK5/p35复合物的抑制阻止了由糖毒性产生的胰岛素基因表达的降低(Ubeda,M.,等JBiol Chem 281,28858-64(2006))。CDKAL1在胰腺β细胞中CDK5/p35的抑制中发挥的作用可能与CDK5RAP1在神经元组织中的作用相似,这种推测是诱人的。因此,CDKAL1表达的降低或抑制功能的降低可能导致对糖毒性反应的受损。在本研究中,我们显示了CDKAL1在大鼠的胰腺β细胞株INS-1中得到了表达(图6)。需要进一步的研究来确定CDKAL1对T2D的增加的风险的影响是否是通过这条途径进行的。
根据CDKAL1的预测的功能和SLC30A8的已知的功能,我们可以预计rs7756992和rs13266634都影响胰岛素分泌。为了评估两个SNPs对胰岛素分泌的影响,我们在经历了口服葡萄糖耐受测试(OGTT)的来自Inter99研究(丹麦B组的一部分)的一组个体中分析了基因型状态对校正的胰岛素反应(CIR)的影响。对于rs7756992来说,我们证明了风险等位基因的纯合携带者具有比杂合携带者或非携带者低大约24%的CIR(P<0.00001,图7)。这个观察与变体的疾病风险的近似隐性状态的遗传相符。此外,在男性和女性(图8)以及T2D患者和对照中都显示了对CIR的影响,并且对BMI状态的调整不影响结果(表21)。rs13266634对胰岛素反应的影响较小但是显著,对于该风险变体来说,CIR的降低符合累加效应。对于每种变体来说,没有观察到对胰岛素灵敏度的影响(表21)。
将CDKAL1鉴定为T2D的易感性基因,为遗传因素如何能够造成对T2D的易感的拼图增加了新的一片。尽管该基因的功能仍有待阐明,但我们已经显示出它在胰腺β细胞中表达,并且基因中的变异与胰岛素分泌相关。与CDK5RAP1的相似性进一步表明,CDKAL1在糖毒性条件下可以通过与CDK5相互作用促进胰岛素的产生。总之,我们在CDKAL1基因中鉴定到了以近似隐性的方式钝化胰岛素反应并造成对T2D易感的变体。
表17、在6个高加索人T2D病例-对照组中和来自香港和西非的病例-对照组中SNPs rs7756992和rs13266634的关联性分析
显示了T2D病例和对照的数量(n/m)、在患病和对照个体中的等位基因频率、具有95置信区间(CI 95%)的等位基因比值比(OR)和基于乘法模型的双面P-值。a当将高加索人组和5个西非人组的结果合并时,使用Mantel-Haenzsel模型将OR′s和P值合并,而病例和对照中的频率被估算为不同研究组中的权重平均值。
表19、在CDKAL1中分型的SNPs的两两相关性。
在CDKAL1中被测试了与T2D的关联性的8个SNPs的两两相关性,D′(左下角)和r2(右上角)。对于来自冰岛、香港和西非的研究组的对照个体分别估算了相关性。
表20、rs7756992和rs13266634的基因型特异性比值比。
a杂合的(0X)和纯合的携带者(XX)与非携带者(00)的基因型比值比的比较。b乘法模型(虚假设)与完全模型的测试的比较,自由度为1度。
表22、6号染色体上标记物rs7756992的替代标记物。表中显示了在HapMap高加索人CEPH样品中r2值大于0.2的标记物。搜索在rs77566992侧翼的2Mb的区域中进行(上游1Mb和下游1Mb)。
表23、10号染色体上标记物rs10882091的替代标记物。表中显示了在HapMap高加索人CEPH样品中r2值大于0.2的标记物。搜索在rs10882091侧翼的2Mb的区域中进行(上游1Mb和下游1Mb)。
表24、17号染色体上标记物rs2191113的替代标记物。表中显示了在HapMap高加索人CEPH样品中r2值大于0.2的标记物。搜索在rs2191113侧翼的2Mb的区域中进行(上游1Mb和下游1Mb)。
序列表
<110>解码遗传学私营有限责任公司(deCODE Genetics)
<120>2型糖尿病的遗传易感性变体(GENETIC SUSCEPTIBILITY VARIANTS OF TYPE 2
DIABETES MELLITUS)
<130>SCT091944-50
<160>44
<170>PatentIn version 3.4
<210>1
<211>201715
<212>DNA
<213>Homo sapiens
<400>1
tgatatttct tgtgttttgt ctatctctac ctattagtat ataaactttt ttctttcttt 60
cttttttttt tgtttttgag acagtctcgc tctgttgccc aggctggagt gcagtggcac 120
gatctccgct cactgcagcc tctgcctcct gggttcaagc aattctctgc ctcagcctct 180
tgagtagctg ggattacagg catgcaccac cacgcctggc taatttttgt atttttggta 240
gagacggggt ttcaccatgt tggccaggct ggtcttgaat tcctgacctc gtgatccacc 300
cgcctcagcc tcccaaagtg ttgggattac aggcgtgagc cactgtgctc ggccgcatat 360
aaattcttaa aaggggaatg atattgtctg ttttattttt tgagcattta gaacagtact 420
tggtatactg tatatactca ataaatacat tattatgtaa ctcaataagt ttgcaatgta 480
tgagtcatat attactgaag gacagagaaa acaacgcatg tcttctaccc acagataatt 540
ataagcaatc agggagttag acaacagatg cgaattctta tttttttaat cattaagaga 600
atatgtaaca gcaagaagtt tagccagcac tcttctggtt gcaagtcata gaaatccact 660
caaattagtt taagaaaaga agagaaggat ttacttgtta acataactga atagcacaaa 720
aagaaagcta gctccaggcg tgctgggtat aggggcctta caattgtcct tggaacttat 780
tgatttctcc cttcatctct caactctgct ttcctctgaa agagctttgg taggccgggc 840
gtggtggctc acatctgtaa tcccggcatt ttgggagtcc gaggcgggtg gatcatgagg 900
tcaggagatc gagaccatcc tggctaacac ggtgaaaccc cgtctctact aaaaatacaa 960
aaaattagcc aggcgtggtg atgcatacct gtagtcccag ctactcggga ggctgaggca 1020
ggagaattgc ttgaacctgt gagacggagg ttgcagtgag ccgagattgt gccactgcac 1080
tccagcctgg gcaacagagc gtgactctgt ctcaaaaaaa aaaaaaaaaa atagcttcaa 1140
tctcaggccg gtgctctcct tgtggtagtc ctggaagctt ctgcctcaga atttcacagt 1200
tgaccgacaa ccccgaattc acgctggcct ggtgttggtt gcattccctg tgatgttctc 1260
atttctctgg cttgggctac agggcacttg tacaaccccc aaaacacttg tactgagagt 1320
gggagagtga acagataaaa ttaattatag aattgaacca aacatatctg tgtaagttga 1380
tagagtagaa tagatgatag taaagctgaa agaggaggtt caagcctgta atcccaatat 1440
tttgggagac tgaggcggga ggatcacttg agctaggagt ttgagaccag cctgggtaac 1500
agcaagactt tgtctctacc aaaaattaag aaaattagct aggattggcg gtgtgcacac 1560
ctatagtccc agctacttga caggctgagg tgggcaggcc gctggagcct gggaggtcga 1620
ggctgcagca agccgtgaca gcactactgc atgccagcct gggtgacaga gccagaccct 1680
gtctcagaaa aaaaaaaaaa aaaaaaaaag ttaatagaag tatagcactc tgacccctag 1740
tggttaaatt atgcagagaa aaattcagga cttgtccaaa gacaacagga ggttgattta 1800
tttcacatta tgttcctgta tgatcttttt atcagtttat ttaagagcat cagctttctt 1860
cccttttatt ctccacattg aagtatccat ccatttgggg caatttttcc tttcacatat 1920
atctagtatt tctctcgccg cccgcccccc ccaattcctc tgctgttacc atatttcttc 1980
ttgggttcat ccaacacctg aaaaatcttc ctaaaaaact gttcagttgg ccaggcacag 2040
tggttcaggg ctgtaatccc agcactttgg gaggccgagg cgggcggatc acctgaggtc 2100
agcagttgga gaccagcctg gccaacgtgg caaaaccctg tctctactaa aaatacaaaa 2160
atgggccagg cgcggtggct caggcctgta atcctagcac tttgggaggc cgaggcaagt 2220
ggatcacctg aggtcaggaa tttgagatca ccctggccca tgtggggaaa ccgcgtctct 2280
acttaaaaaa aaaaaaaaaa aataggcggg tgtggtggtg ggcgcctgta atcccaggta 2340
ctcaggaggc tgaggcagga gaatcacttg aacctgggag gtggaggttg tagtgagccg 2400
agatcacacc actgcactcc aacctgggtg acagagcaag actccatctc aaagaaaaaa 2460
aaaaaaaaga tagtagaagt atagcactct gacccctagt ggttaaatta tgcagagaaa 2520
attcagcatt tgtccaaaga caataggagg ttatttcaca tcatgttcct atatgatctt 2580
tttatcagtt tattaaagaa catcagcatt cttccctttt attctctaca ttgaagcacc 2640
catccatccg gggcaatttt tcgtttctca tagatctagt atttctcttt ttgcccccct 2700
ctattcctct gctgttacca tctttcttct tgggttcatc caacacctgc aaaactttcc 2760
tgaaaaactg tttagttggc caggcacagt ggttcagtcc tgtaatccca gcactttggg 2820
aggccaagat aggcggatca cctgaggtca ggagttcgag accagcctgg ccaacatggt 2880
gaaaccccgt ccctactgaa attacaaaaa ttggctgggc acggtggcat gctcctgtaa 2940
aaaaaaaaat taagagcact taatagtcat acttttcact ttgcttatat gtagcttata 3000
tatatataaa tataaaaatc tgttttaact ctcattctac taaattcacc taatgttcta 3060
catagaaaat ttcatgtata ttgtttatca tctttcaaga gtgaattaag cacatgaata 3120
ttttctcatg aaaagggata ttgattggta cagatgtgta tgtataatgg ctagttgctc 3180
agatcatcaa ctaaatctaa aacatgtgtt ccagctgggc tggtggctca tgcatgcaat 3240
cccagcgctt tgggaggttg aggtggagga ctgcttgagg ccaggagttc aagaccagtc 3300
tgggcaacat agtgagactc tgttgctgca aaaagagttt taaaattagt cgagcgtggt 3360
ggcatgcccc tgtagtttca gctacttggg aggcagaggt ggggggactg cttgagccca 3420
ggagttcaag tttccagtga gctctgatgg tgccactgtt ctccagcctg ggtgacaaag 3480
ccagaccctg tctccaaaac ataatcttaa aaagcagttc ccaattctta attttaattt 3540
tatttattta tttttgagag agggtctcat tctgctgcct ggactggagt gcagcggtgt 3600
gatttcggct cactgcaaac tggaccttct gagctcaagt gattctccca cctcagcctc 3660
ccgagtagct ggaaccacca tgtccggcta cttttttgtt tgttttgtag agacagggtc 3720
tggtcatgtt gcccaggctg gtctcaaact cttgggctca agtgaaacaa ctgcctgtgc 3780
ctcccaaagt gctaggatta caggcatgag ccaccgtgcc tggccccaga tttttataat 3840
tttcatattt tattctttat tgattagtca ctgagagggt aagaattgtg taacttcttt 3900
cttgtccata ttttctcttc aatttttact gtgaaggcca acataaaata tttaaggcaa 3960
acagattttt tttttttttt tgagatggag tctcactgtg tcacccaggc tggagtgcag 4020
tggcgcgatc tcggcttact gcaacctcca ccttccaggt tcaagcgatt ctcctgcctc 4080
agcctcccga gtacctggga ctacaggtgc ctgccaccgc gcccggctaa gttttgtatt 4140
tttagtagag acgggatttc accatcttgg ccaggctggt cttgaatttg tgacctcgtg 4200
atccacctgt ctcggccccc taaagtgctg ggattacagg catgagccac tgtgcccggt 4260
ccaggcaaac agattttatt ccacttttcc ttgatcactc ttttgttttt tctctccaat 4320
gctttttctc tatttgaaat tttgcactat tgttgctttc ttctgaagta ttcatataga 4380
gttgttaaaa caactgaagt cagactgcct aagctttagg tttcttctct gttaaaagag 4440
ataacaagat aataatagca ctcctcattt atagggttac ttatgggtgg ttattggcac 4500
acatttataa gtgtttgaaa aaatcttagc tttactatta ttttatagat ttacttgcat 4560
ttttaatgtt cttggtatac accaattggt ttgtttactt attaccaaat atttataaag 4620
catttagtgt tatattgttg gctgaaaaaa caggaacaaa ataggatggt gtatatttat 4680
gaagcttaac tctaatttaa gaaacaaagc attatttttt tttttctttg agacggagtc 4740
tcactgtgtt gcccaggctg gagtgcagtg gcgcgatctc agctcactgc aatctccgcc 4800
ttccaggttc aagcgattct cctgcctcag cctcccgagt agttgggact acaggctcat 4860
gccaccatgc ctggctaatt ttttgtattt ttagtagaga cagcgtttta ccatgttagc 4920
cagtatggtc ttgatctcct gacctcgtga tccgcccacc ttagccttcc aaagtgctgg 4980
gattacagcc gtaagccatc gtgcctggcc tttttttttt ttttttaatc tttttgagat 5040
ggagtttcgc tcttgttgtc caggctggag tgcaatggtg taatctccgc tcactgcaac 5100
ctccacctcc tgggttcaag agattctcct gcctcagcct cctgagtagc tgggattaca 5160
ggcatatgcc accacaccca gctaattttg tatttttagt agagacaggg tttcaccatg 5220
ttggtcagtc tggtcttgaa ctcttggcct caggcgatcc acccgcctcg gcttcccaaa 5280
gtgctaggat tacaggcgtg atccactgca cccggactta ctggttgctt ttcaaaggtt 5340
gtacagatac tataaatata tttaaccaat actctattga ggaggagccg gagagggttt 5400
ccagtctttt gctattgtaa gcaatgctta aaaaaatact ctggaacata ggtcattgta 5460
agcaatgctt caaaaaatac tctggaacat aggtcattgt aagcaatgct tcaaaaaata 5520
ctctggaaca taggtcatta tgtatatttc tgtaagataa attcctagag gtagggttac 5580
tgacttcaaa ggatttagac taaaggtgct taaagagaga ggtttcacct gttagttagt 5640
ttttgtagcc aaaacttgaa aacatccttt cttcctcttt tttcgtacct atcaatcacc 5700
aagtcttgct gctgttacct cctcaatttg tttaaatctg tccttccttc ccattcctcc 5760
cagccaacaa ctcaatttag gcttttacta tttcttcatg tctgaaatat cgtcctcaaa 5820
cccattgtcc tgagagaata atctgctcga acacattcat gttgcctact acatacatcc 5880
aggattaagt ctaatgcctt tgaatacaca aggttcacct tgatctagtc cctgtgcttc 5940
cctttctctg gtagacttct cccatcacca gcttcactct ctttgctttt acactgtgga 6000
ataattcatc atttcaggcc tccttgcttt tgctctggct gactctccag cttggaatgc 6060
cttcatccat ttcccttact tcacctttgt gttcctgtgc attcatcttt catagtgatt 6120
gctattctct actgaaatga attttttcaa aatagcctgc aattgcaata ggcagtactt 6180
tttttctttc tttttagctc tagcatctat cacagtgctt gtggtcatgg ggtacatact 6240
cagtaaaata tcttttactg aactcaacta gatcttgggg gaaaaggggt ggagtcattc 6300
attcattcat tcattcattc aacatatgtt tattgagaag ctataaacat aggcacaagg 6360
ttatgtgcta ggtagtgcga ttacaaccaa gaagataacc cagggcctac ttttggtcta 6420
gtgaaagaga tggaattaga tgggtatggt ggtgtgcacc tgtagtccca gctacttggg 6480
aggctaaggc atgagaatct cttgaggcta ggagggagag gttgcagtga gctgtgatgg 6540
taacactgta ctccagcctg ggtgacagaa gaagacccta tctaaaaaaa aaaaaaaaaa 6600
aaaaaaaaaa gtagggcaac ataaatacac aattttaatt taggtgggta aagacaacag 6660
tggaccccag ggcacagttt agattatttt atggatatgg ataaacagag aaaagggtgg 6720
tttttttttt tttttttaaa tctattttag agactgggtc tcactgtgtt gcccaggctg 6780
ttttcaaact tctgagctca agtgatcctc ccacatcagc ctcttgagta gatggatggc 6840
attttaatta gatgaaattg tactaggaaa ggcatttgca aaacaaagta attgccccaa 6900
atcactatga aaccctaagt caggcataaa actcacgatt tctgaccctt cttcatggcc 6960
tattgcctcc tattaccttt actttttcaa gtaaagatat tcttctggta aacacatggt 7020
caaatacagt gtcttgggta acgaatagta aagtgagata acatatgtag agaaagcggg 7080
tgagaatccg tactccataa aagtattttc tttccatagg tgtctgctaa actgccatct 7140
aagttttcca aaacaaaaaa caaaaacact tgtgtggtgc tcaatgatgt atattaagtg 7200
aaacctacca actgaccttt atcctttcct tcctgttttt ttaatattaa agaagagacc 7260
tcttttcaca aatgattgag gaggctagta aaatgcagtg aaaagagcaa gttctttggg 7320
agtcagacag catcggatcc agcccagagc gcagcacgac tagtagagta acttagatta 7380
agtccttaaa cttccagtgt cctcttttgt aaactgggaa taataataat aataaataaa 7440
tagtgaataa tatttatgca aagtacttag cacagtgtct aacacataaa agcttaggag 7500
aagttggcta ttaagcattt ctaggaaccc gaggactcag ggttgttatt ttagttcttg 7560
gaatcaaagg aacttgaagc gtagtcattc ctaaactaag ttaaaccaga actagcgctt 7620
tcagcaatga tgtaatttga tctcttcggg ccaccgtcgc tgagcatgcg caaataaacg 7680
tggcgggacg tatgtgtcat ggcgctctcc atctaaagtc tgtgcagctt ccggagagtg 7740
gcgggttgat tttctcactt tggactggtt tttacttccc gacttctggg taagggtggc 7800
cgatgggtcc atgtggggca gagtgtggcc acagctcctt ttatggccaa gccttgtttc 7860
tccagtttca gtttttcttg ggctgtttgc aaatttgttt cgcagttaaa aggggatttg 7920
ccagctggga tgggggaatt gggaggcaga tggggcttcc aggagcgagg atagggtcgt 7980
tggcctcagg tgccgctctc cagttaggag tattttaggc acctcgttcc ttattgtcag 8040
gtttaacttc atttgttctc ccactttata ccttagtgaa tttgtagatg tgacaaggct 8100
ttcgcagtta tatagctttc ccagatcagt atgttaagaa tttaagcaga agttattgag 8160
aaaagcaggt gtcttgcttt ttcacttttc gttcttcttt gctcataaaa cctttttctg 8220
aatctcggga cgacagttat ctttcggatg gaacgtgatc tggatgtcta tctaccttat 8280
tgacttaatc ctgttttgta gtggttggtt actcgcacta tgcagacgtg ttgcggtgct 8340
gaatacaaca tttattacta aagccagaca ctggatcacc gtcacggcag atgtcagcac 8400
acctgttgta atcagtaaga agttacaagt agtatggagc atcattaatc ctgctgtata 8460
aagcagtctt ttatggagtc tgattccttt taagtaaaga atccttacag tggttttgtg 8520
aactttttgg gtgcttactt ttcctaatca atttttattt tccagactca tctttcaaga 8580
ggactttaga ctaattgcag ataattaagg tactgattta ttttaaacct tttatgtttg 8640
agtgctgtgc gtatttctgc atgttagaaa atgcagagag gtagcctaat gttttagcta 8700
tttgccacta tcatgttatg gaaacattgt gctttacata gcattaaatc gaagagatat 8760
tttcatttat tttacttaac ctttcatttt cattcaccag cattgaccat gtccactttt 8820
ttgagatgcc cttttcattt gccttccatg gcccatggat ttttttcctt gtctggtggc 8880
tgcttcttgg aatccattat aagctgattt tcctttatct ggcagttaaa tttggagtct 8940
ctcaagtcgt ggtcttgtac cttctcctac ctctgttctc tttttcttcc cctctgggtg 9000
atgtaatcca catgcctgtc aacagttatt tgtctttttg attctaccca tcctaatggg 9060
tatgaagtgg tgtattattg tggtttggat ttccatttcc ctgatggctc atgatgttga 9120
catcttttca tgtgcttatc agccatttgt atgtgtgtgt ttttgagaca cggcttccct 9180
atgccaccca ggccagagtg cagtggctcg attacggctc actgcagcct tgaccacctg 9240
gtctcaagtg atcccacttc agcctcccaa gtagctggga ctacaggtgc atgccaccat 9300
gcccggctaa attttacttt tttttttttt tttgtagcga tagggtccgt gttgcctagg 9360
ctctggcctt gtactcctgg cctgaagtga tcctgccacc ttggcttccc agtgttggga 9420
ttataggcat gagcctctgc acctggccca tttctgtatc ttcttcatct aatctgtttt 9480
tccttttgtt gcttgtgctt ttcatttctt agctaagaaa gaagggccta ctctaacatc 9540
ataaagattt gtgcttatgt ttttttctaa gagtttcata atgttagctc ctaaatttag 9600
gtctatgatc cattttcggt taatttttgt atatgatgtg aaatgtgaaa tagggctcca 9660
actttatttt ttacatgttg ctatccagtt atcccagtgt catttgttga aaaaaaaact 9720
acgctttccc ccttgaattg tctaggttat gtatatttga catacattct aggtggtttt 9780
tatgaaagaa taattctggg acagtgatta tcaaacttta attcagatca aaatcaccta 9840
gagggcctgt taaatattgc taggttttac tcaaaatatc tgatttagta tgtctggagt 9900
ggagttcaag aattttcatt tctggctggg catggtggct catgcctgta atcccatact 9960
ttaggggcca agatgggagg agtttgagac cagcctggac aacaaagcga gaccttgttt 10020
ttacaaaaaa actgaaaaag aaaaaatgtt catttctaac aagttcccca tgatgctact 10080
ccagctgatg tggaaactac actttgaaaa ccactggaca ttgctttgca actgtggctg 10140
tacacacatg agaatgactg gaaagttttt aaaagatatt gatgcccagg ctccattctc 10200
agagattcat tcagctatct tggggcttag ttgggaattt tctttttttt cttttgtttt 10260
tgtttttgtt ttgagacaga gtctcgctct gtcgcccagg ctggagtgca gtggcgtgat 10320
ctcggctcac tgcaagctta gttgggaatt ttcaaaagct cttcaggtga atagaatgag 10380
tgttgaagat tgagaaccat tactgtggaa tcgtggttct taattaggga tctatagtgg 10440
aattacctgg acagtttcta tgttcacata cttcagttct actcttggag attctaattc 10500
agaatggaac ctggagattt gatatgatga aaagcttccc gctgggtgca gtggctcacg 10560
cctgtaatcc cagcactttg ggaggccaag gcaggtggat cacctgaggt tgggagttca 10620
agaccagcct gaccaacatg gtgaaacccc gtctctacta aaaatacaaa attagctggg 10680
cgtggtggca ggtgcctgta atcccagcta ctcgggaggc tgaggcagga gaattgcttg 10740
aacccaggag gcacaggttg cagtgagccg agatcgtgcc attgcactcc agcctgggca 10800
gcaagagtga aactctgtct cataattaca aaaaaaaaaa aaaaaagcct ccctaataac 10860
tcacctatgt aagtaaccac tgctctagga tattgcatcc tccttcttcc tatcattttg 10920
agtattttcc ttttcactga ctctattttt attcccttta tcaaatttga tttcctctac 10980
ctttgagaaa aatttttaat gatccttttg ctccatttag ctattacgct gctttccttt 11040
tattctcaaa ctttcatttc catatgattt aattaaaaat ttctgaaggc taaacaattc 11100
cctctatcag tctatcagtt tgttcaaagt aaaaccttta gcttattaat agtgatgagt 11160
gagattattt aggtacttat gatatacagc caggtttatg ctacataatg ctgatatgca 11220
gcgttatatt tatctttttt gagtgaatat ctccaaatga aattgctggc tcgaaggata 11280
tacatatgtt tggtataatt gtcaggttgc cctccagaag gggtgtacca gtttttctgt 11340
gctttcagct aacagagttt ttgtcagctt tgtatcagtg aggcagctga aaaataactt 11400
attgtttcaa tacttgttgt tagtgtggtt gagcattctt tcctcttttt ttttttttct 11460
tttctgggta tttgtatttc ttaagtgaac tgcctgtttt tcctttacgc attgttttct 11520
ggaatgtggt tacttattag tgtcacatta gattcttgca ttgtttgtac tttgccagtg 11580
aggcaaagcc atagtgggaa gagcatggta ttgagaggat gccaaggttc tgatttctag 11640
ctctatcaat gtacaaatcc ttttgcttca gtggactcca gctctgtaag ataagaagct 11700
tagcaacttc tgcttctcta actctaatga tatgggatgt ctattgcagt ggattccttt 11760
cattcctctt tttcctttct atttctcttc agtggatcca tacacacttg tatatgggat 11820
ggcaaaattg tgtaattaaa aaatatggct tttaattaat gttatttaat tattcatgat 11880
ctgtcagtag ttcttccgca ttccaaaggc actacctggt cctagcctct ctcatttaat 11940
tatttcatag aagagtggtg atagaagaaa tcctaggggt tgggcattta cttcagcttc 12000
ccacccagtg tagaatttcc tgctgtgtca tagaaagact agctaaattc aacttaagca 12060
ttgtgttcat tcatttagtc agtaactatt gtgtgcctgc tatgtgtcag gcattgttag 12120
aggtgttgta gagacagtac tgaaaaagag atgatgcctt tgtgcttaac aagcttacat 12180
tgtaatgagg aagatacaat tatatatgtg gtgtcaggta ggtaagcaca atgaagaaac 12240
atgaaacaaa agcagggtta gaagcagtga aagtgtgggc atgcttgggc agcgtggtta 12300
ttttagataa ggtagtagga aaggcctctc cgaggagatg ttgtttgagt gaagaagtgg 12360
gtgggaagac atacttatct gggggctgat attctggcag aggaatagca attatgaagg 12420
ctcccgagtc agaaactaat ttggcatact cagaatatct atatccatat attgcccgac 12480
tgtgtagtct gcagtaaaag tatttattga gaacctgcaa tgtgctagac atagttctag 12540
gcacctggga taaaacagtg aacacctcac acgaaaacct ttaccctcac gaagtatgta 12600
ttctagtaag ataaacagtt aaaaaattat taaaacattt taagcagatg attaaatgct 12660
acagtttaaa aaaaggcagg aaaggggcat aggaagaggg tgtgtggtga ggtgggggtg 12720
ttggttggaa agtcctctct tgttacactt gagcaaagtc cttaagggct tgagtgagtg 12780
agccattcag gtgtcttaag taagagcatt ccaggcaaga ataagactgt gaaatgaatg 12840
catatctagt gggtacgaaa taacaaagcc tgcatgactg aggggagcgt ggtaggaagt 12900
ggggccagag atgcagcagg aggctgatta tatatgtcat tgcagatctt tgtagtgatt 12960
ttggcttttt actctgatat ggaataccac tggaagggtt tgattgagaa gcttgagatg 13020
aacttaggtt ttaaagggat ccctgacttc tgtgctgaaa atgttcttgt agagtttaag 13080
gatagaagca aggatattag ggattgcaat aatccagaca agaggtgatg ttggtttgga 13140
ccaagagtgt aacagtgggt atggtcacaa atagattctg aatatatttt gaaggtacag 13200
ccaacaggat gttgttgatt gattggattt agggtgacag aaagagggga gtgcaggatt 13260
tttggcctga gcagtagaag attgggattg tctttttttt ttttttgaga cagagtctca 13320
ctctgttggc cgggctggag tgcagtggtg agatcttggc tcactgcaac ctctgcctcc 13380
tgggctcagg caattctcct gcctcagcct cccaagtagc tggggttaca ggcgctcgcc 13440
actatgcctg gctaattttt tgtattttta gtagagatgg ggtttcacca tgttggacag 13500
gctggtcttg aactcctgac cttgtgattc acccacctcg gcctcccaaa gtgctgggat 13560
tacaaacatg agccactgcg cccagccagg attgccatta aatgaaatgg gagaagttgt 13620
aagaggatct ggtaaggtag ggagaagatc aggaattctg tttcgacttt gacttttttt 13680
ttttttggga tggtgcttcg ccctttgtcg cccaggctgg agtgcagtgg tgcaatctca 13740
gctcactgca acctctgcct cctgggttca agcggttctt ctgtttcagt ctcccgagta 13800
ggtgggattg caggtgccca ccaccatgcc tggctaattt ttgtattttt agtagagacg 13860
aggtgtcacc atgttggcca ggctgacctc caactcctga cctcaggtga tccatccgcc 13920
tcagcctccc aaagtgttgg gattacaggc gtgagccacc atgcccagcc ctgttttaac 13980
tttttaagtt tgaaggaatt attagacatt caagcagtgg tgtggaatag gcagttgaac 14040
gtctgagtct gaagctcggg ggaagaatgg ggctggttat gtaagcatag acgtagttca 14100
tatttcaagg ccatgggact ggatgaggtc acctagggag tgcctgaaac tagagaagag 14160
ttcctgtaac agagccatgg gggcattcta atggtgagaa gttggagaga tgagcagagg 14220
agactggagc taggagagtg tggcatcctg gaaactagtg caatttctta ctgcagttct 14280
tcagtcttta ataatgcaca ctgtgaatgg gtaaggacgc agaattttct aaatttattt 14340
gaccacagaa ccattttttt ttccagatag gtagtaaata attagaagca atttgggaaa 14400
gactaacctg gttcatttca ccctacctgg atggctgagt tggccagggc tcttttttgc 14460
aagaacagaa tacccacctc aaactgattt aagccacagg ctcgcatgac tgggcagagc 14520
agggctgcat ttggcttcag gcatttgttg gattcagatg ctcaaataat gtcagtatgg 14580
ttctttatct ttctagatct tattaggctt cattccttaa gtaggcttcc tccctgtagc 14640
agacaagagg gctctggtat ccccaagttg acatcctccc aacttagcca cttcagtgac 14700
aagagaagct atcttctgtt aacactagcg tagaatcctc acagtgattc ttattggcta 14760
ggcttctcat gactgacctt gaactggtca ttgtggccag gaggatgagt taccctggca 14820
agcttgggtc atgttcccca ttccagtgtc tagggaaagg gaagtctgtg attgacagtc 14880
tttttttttt ttgagacaga gtttcgctct tgttgcccag gctggagtgc aatggcgcga 14940
tctcggctaa ccaaaacctc tgcctcccgg gttcaagcaa ttctcctgtc tcagcctcct 15000
gagtagctgg gattacaggc atgtgccacc acgcccggct aattttgtat ttttagtaga 15060
gacagggttt ctctatgttg gtcaggctgg tcatgaactc ccaacctcag gtgatctgtc 15120
cgccttggcc tcccaaagtg ttgggattac tggcgtgagc caccgtttcc ggcaattgac 15180
agtcttacca tgaccacgtg gtacgtatgt taactataat ccagccaatc cttgatggat 15240
tgatgtttgg tttatttcta atctttggtt cttactgaca atataaagct agctgagatt 15300
tgaacacaga tttttcggat acccagtgca taatttgtca tagctgtaat gaatacatta 15360
cagaggaaag gagcctggct ggctgccact ctgttaattg aatgatgtga atggaaggat 15420
gggaatccag caaatgagaa ggaaaattca tcagaaagct ttgaggacaa ctgaaagaat 15480
gtaagatcct taacactgca actcagatga ggaacagtgg tcattcaata tacacatact 15540
aagtaggtag tagagtacca cacagtgcgt gggcttggac tcatctgtca tggtatcctt 15600
gtttagcttc tttgggcaag ttaatttatc tgtgtttcag tttgttcatc tttaaagcga 15660
ggatagtaat actatctacc tagtaagagt tgttgtgaat ggttgatgag atgaccctat 15720
aaaggacttg ccttgtgtct ggatatagta ttagtggtag tactatcagt aagtatatac 15780
gagtgtattc cccccccatt tttttcttaa ctattttatt ctaacataat taccgcttta 15840
cctgcagttg taaaacttat gcagagatcc ttgtacactt tacccaggtc tcccgttggt 15900
aacatcttgc aaaactacaa tacaatacag tatcacaacc aggatattga cattgataca 15960
gtcaggatgc aaaacatttc catcatcaca aggattcctc atgttgctct tttatagtca 16020
tacccattct cctctcaccc tgttctttct ttaatccctg gcaactacta ttctgttctc 16080
catttccata attttctcat ttcaagcatg ttatataaaa gtgatcatac agaatgtaac 16140
cttttgggat tgaatttctt cattcagcat aattctcagg agagtcatcc aggttgatgc 16200
atatgtcatt agtacatttc tttttattgc tcaatagtgt tccatggtat ggatgtacca 16260
cactttgttt aaccattcac cttttgaatg gcatccaggt ggtttccaga tttgggctat 16320
tacaaagaaa gctgctgtga acattcatgt acaggtcttt gtctgaatac aagtctttat 16380
ttctcttgga taattgccca ggagtacaat tgctgggttg catcacagtc atatgatttg 16440
tttaagaaaa caccaaactg tttactggag tggctatacc atttttcatt ccctccagga 16500
ctgtatgaat gatgcagttt ctttgcatcc ttgccggtgt ttggtggttt cactgttgtc 16560
tgttttatcc attctgaaag gtgtatactg atacctcatt gtggttttag tttccatttc 16620
tgtgcttatt tgccctctgt atattctctt gaaatgtctt ttgtcatttt ctttttacgg 16680
ttgtgttttg acactagttc tttgtcagat atgtggtttg caaatatttt cttccactct 16740
gtagtttgac tgtttgactt ttcagcctct taatagggtc tttcttaaag caaaagtttt 16800
aaattttaat gacattcaat ttattacttt ttctttttat ggattgtgct tttggtgtca 16860
catctggtaa ctttttacct atccctaagt ccagaagatt ttctcctgtg atttttttct 16920
aaaagtttta taatattatg ttttacattt tttttttttt tttttttttg agacagagtc 16980
tcactgtgtc acccaggctg gagtgcagtg gtacaatctt ggcttactgc aacctctgcc 17040
ttgtgggttc aagcaattct cctgcctcag cctcccaagt agctgggatt acaggcgtgc 17100
accaacacac tcagctaatt ttttgtattt ttagtagaga cgcggtgtca ccatgttggc 17160
caggctgttc ttgaactcct gacctcaagt gatccacacg cctcggcctg ccagagtgct 17220
gggattatag gtgtgagcca ctgcgcccag cctatgtttt acatttaagt ctatgatcca 17280
ttttgaatta atttttgtat aaggtgcaag acttaggtca aggttcattt tttgcctata 17340
aatgtctgct tgctccagca cgaattgttt aaaaagcaat ctttcctcca tttaattgct 17400
tttgtggaat atttgtgtag gtctgtttct gggttttctg ttatgttcca ttgatctata 17460
tttctatccc tctaccaata ccacggtctc aattatagct atctaataag tcttaaaatt 17520
gggtagacca ggccgggcgc agtggctcac gcctgtaatc ccagcacttt gggaggccga 17580
gacgggcgga tcgcgaggtc aggagatcga gaccatcctg gctaacacgg tgaaaccccg 17640
tctctactaa aaatacaaaa attagccggg catggtggcg cgcgcctgta gtcccagcta 17700
cacgggaggc tgaggcagga gaatggcgtg aacccaggag gcggagcttg cagtgagtcg 17760
agatcgcgcc actgcactcc agcctgggcc acagagcgaa actccgtctc aaaaaaaaaa 17820
aaaaaaaaaa aaaaaattgg gtagaccagt tcttcttact ttactcttct caaaattgtt 17880
ttaaatatat tagtttctgt acatttccat ataaaattta ggatagtctt atctatacct 17940
acaaaaaaat cttcctggga ttttgatagg gattatttta aacttgtgta tcagtttggt 18000
ctccactgac actgcaaaac ttcattactg gtcggttggt tggtttgaat gtccaggatc 18060
cctccaggtc cttctctgat tctacactag tggagtgtgt tggggtgctt gattgtaggt 18120
agaagtccaa gctctcccca cttggccttt gtagcgtgcc tgggggtggg attacagttt 18180
tgtgtgtgtg tgtgtgtgtg tgtgtgtgtt tgtgtttggc tggagtagaa tgattattat 18240
ctaaaacttt tctgtcttcc taggctgccc tttgtatagt tctttaacga gaaagcaggc 18300
ttttattggg gggttttatt gttggcatct cttgccattt ctgattgctg ggatctttag 18360
tgagctgtag gaggcagaag aaaacccaag gaactcatca tattccttgg gtcctgaggt 18420
tcatagccag tctgccttat gcttgtttta tatataatgt ccagagtttt atttgtattt 18480
tgctggcggg gcagggaaaa atgtgcttat cttcttgcaa gctgtgtcca cattttcttc 18540
ttttaattat aaaaattttg aatatttaga aaagtcgtat aggataatga acatgaacct 18600
atctagtttc tagttttaag agttgtaaat gcttttcagt atgtcttaat ctattttttc 18660
ttcttttttg agatggagtt ttgctctgtt gcccaggctg gagtgcagtg gtgcactctc 18720
gactcactgc aacctctgcc tcctgggttc cagcgattct ccttcctcag cctcccaagt 18780
agctaaaact gcaggtgtac accaccatgc ctggctaatt ttgtactttt aaaagagatg 18840
gggtttcacc atgttggcca ggctggtctc aaacgcctga cctcaagtta tgcgcccacc 18900
tccccaaagt gctgggatta caggcataag ccacccaaag tgctgggatt acaggcataa 18960
gccactgtgg ctagccagtc tattttttct gaggtccttt taaagtaaat tatagacata 19020
atgacatttc acttctaaat attcaatatg catctctaaa aaataaaatt tttgaatata 19080
aggacaatac tttatatcga atgatttata ataacttctt catattatct aatatccagc 19140
ttatattctg atttccctag ttatacccaa aatttcttat agcttgacag acaaagaaga 19200
aacaatcaag aacatgaact gcattttgtt gttaggcttt tacgcttcct gttatctgaa 19260
acagttcctc tcctactttt tcctacttga cattgatttg ttgaagaaat agttcctgtt 19320
gaaccttgga atgtcccatt gtcattaggg ttttgtctga ttgtttcatt gtgtttcttt 19380
tttcaatata cccaagatag tacttcatcc ttgtgtttct ttatctcttg tattttctat 19440
aaatttgaag ttagatccaa aggcttgatt agattcaaat ttggtgagaa tatttcataa 19500
atgatgctac gctaagcaga ttttcataag ttgattttat ttataacttt tatgtggtag 19560
agaatatgcc ttctgcatcc tgtgatacac tactggatga catcgaagat atcgtgtctc 19620
aggaagattc aaaaccacaa gataggcatt ttgtaagaaa ggatgttgtc ccgaaggtac 19680
gaaggcgaaa tacccaaaaa tatttgcaag aggaagaaaa cagtccacca agtgacaggt 19740
aagctttttt ccttgaaatt atattcattt tctttttttt tcttttgaga cagagtcttg 19800
ctctgtcgcc caggctggag tgcagtggtg tgatctcggt tcactgcaac ctcctcctcc 19860
tggattcaag ctattctcct gcctctgcct ccctagtagc tgtgattaca ggcacacacc 19920
accatacctg gctatttttt gtatttttag tagagatggg gtttcaccat gttggccagg 19980
ctggtcttga actcctaacc tcaggtgatc tgcccacctt ggcctcccaa agtactggga 20040
ttactggcct gagccactga gcacggccga aattatattc attttcattc cagggagaag 20100
ctgacgctac tggtctgtgt gatatatata atgtctcttc attagaatct ttgaactttt 20160
gataggaaat acaagttgag tatcacttat ctgaaatgct tgagaccaga agtgtttcag 20220
atatcacatt tttttttttg gatttcggaa tatttgcatt acatactttc cagttgagta 20280
ttcctaatcc aaacatctga aatgctccag tgagcatcct ttgagtatca ttttggcact 20340
caaaaagttt cagattttag agcattgtgg attttgaatt tttgaattaa agaaactcaa 20400
cctgtatgta gatttttttt ttatttgaat gattttaaat ttggcaaggt aattggttaa 20460
aaagaaatga gatgtcaaat tggatgttcc tagaagatag cctcgggcaa tagggatctc 20520
ttgggtatta ctgtagttga tatgtaagtc tgtgttattt ccaaataaaa tttttaccag 20580
agtttgtgat tcttatgaga cagtagtatt ttggacattt gaattttctt agctgatttt 20640
caaatacctg tggatcaggt aaaggcagtg ggcacaaata aattattgcc ctatttacgc 20700
tattatagta gatctctagt ccgtgaaatt ttgatacaac ctaaccagaa aggataatgt 20760
aattatatgg ttttaataag cctatatttg tttatctagt caactgctga actttataat 20820
ctgcttattt tgttgggaaa ctaagctttt aaattcttgg aatttaaaat ttgcttatat 20880
tttaatgact gaagtttata aaatttgtcc taaatgtgtc cttaaggtaa tttttattta 20940
cttataaatt attagagcaa tccaagttga aagtcctttt ttcccctaaa agttattttt 21000
agaactctga ttttatagag tgcttttaat aatgctttag gatgacggtt tttaactttg 21060
attaagttac ttatatctgt aagttttaag gaagctacag attgcaacta gtatgtagga 21120
atctgtaaac agacatactt ttctttattt gccattgctg ctagaaccac tttctttcct 21180
attgttattt ttgtgtacct ttccaggagt tgaacttagt aagttagtag tatgaattta 21240
aaatgcaatt cagagaaaaa ggatattttt ttatgtggag tgttaacttt ttagaattct 21300
gtggttattt tatcttgatt ttagactttt gatacaacaa cagattttga gttaagttgg 21360
tgcgtttaat gaatagcttt tgaatggatt gtagtgagtt tgttttttgt gtaatatatc 21420
ttaaaatcat ctagtggttt ttattttaaa aataactcaa tgaggttggg tgtagtggtg 21480
gctcatgcct gtaattccag cactttggga gacaaggtgg gaggatcact tgagcccagg 21540
agttcaagat cagcctggac agtatagtgg gccactgtct ctacaaaaaa taagaaaaat 21600
taacaggtgt gttggtgcat gactgtagtc ccagctactt gggaggctga gatgggagga 21660
tcccttaggc ctgggaggtt gaggttgcag tgagctgtgg tcacacagct gcactgcagc 21720
ctgggcaaca gagcaagacc ctggatcaaa aaaaaaaaaa atcactcaat gaaacttact 21780
tttttttttt tattgattcc ttaacagcac tattccaggc atacagaaaa tttggatacg 21840
aacatggggt tgttctcata ataattcaga tggagaatat atggctggac agctagctgc 21900
ttatggctat aaaattacag gtaatgagat ctataatata ttttattgat taaatttttc 21960
agaattaagc ttttagagat aaatagccta gcagttaaag ggtattttag catgacagtg 22020
attttaaggt gaaacagata gttattacac attttgtaga taaccatgga tgtcatattt 22080
aaaaaaactt tttattttga aataagttta gactttcaga aaagtagtaa aattagagag 22140
ttcacatatg cccatcacca agcttccctt gatattaaca tcttacgtag ttgtaatatc 22200
attaataaac caggaaatta acattagtgc atttctgtta tctaaactgt agatcgtatt 22260
tgaattttac cagttttccc attaacttcc tttttctgtc ccaagatcta atgttgggtc 22320
ccaccttaca tttagtttca tgtcccctta gtttcctcca atctgtgaca gtttcttaat 22380
tttttcttgt cttctgtgac cttgacactt ctgaagaaaa catttccttg atatttctga 22440
tgaaaatgat tattatgtag aatgtccttc aacttgaatt ggtctttttc tcatgattag 22500
aatgaggttc tgggtttttg gcaagcagag aacagaagcg atactatatt tttctcagtg 22560
cgtcatatcg gggtcatgat gtcagtgtgt cttatactgg tacactaacc catgtctgct 22620
aggtttcttt ccaatacagt cactgttttt ctcattacaa ctaataaaca tcctatttct 22680
cctcaacctt tggcctgtta attttagtat ctactggtgg atcttgtctg caacagttat 22740
tactgtggtg tttacttaat ggtgattttc tgctatcttg ttttctttac atttatattt 22800
atttatttat ttatttattt atttatttat ttatttactt tgagatgggg tctctttgtg 22860
ccccattctg gagtgcagtg gcacgatctc agctcactgc atcctctgcc tcccaggttc 22920
aagcgattct tctgcctcag cctcccaagt agctaggatt acaggcatat gccaccatgc 22980
ctggctaact tttgtatttt tagtagatac ggggtttcac catgttggcc aggctagtct 23040
cgaactcctc agctcaagtg atccactcgc ctcggcctcc caaagtgcgg ggattacagg 23100
tgtgagccac tgcgcccagc ctcctcttta catttattaa ttggagtttt tctctaagga 23160
agagctgtcc attcttctcc atcttaatta attaattaaa tttttttttt ttagacagag 23220
tctcgctttg ttgcccaggc tggagtacag tgttgtgatc tcggctcact gcaacctctg 23280
cctccggggt tcaagggatt ctcctgcctc agcctcccaa gtagctggga ttacaggcat 23340
gcgccaccac accaggctaa ttttttgtat tttttagaga tggggtttcg ccatggtagc 23400
caggctggtc tagacctctt gatctcaggt gatttacctg ccttggcctc ccaaagtgct 23460
gggattacag gcatgagcca ctgcacctgg cccgtcttag ttatttctat cagtacagac 23520
tcatggatat ttattttatc ctgtggatat agtctgatac tatcattatg tattatgctg 23580
cttgttgttc caatcttggc cattaggatc ttgttcaggc tggctcctgt gtcttttcag 23640
catgccggca tctttttttt gagcacttct ttatttcctg gcaccacaaa atgttctaat 23700
ctctcatatt ttcccttccc cagtcttgga agcaaccagt tttccaagga gtcctgattt 23760
ttttcattaa gagaatggca tttagaaaag gggatctggg cagtcaatat gcttgctcgt 23820
tgccaccgtg aggtgtcgct gttgtgaggt cctcttgggg gatggagcta ggaattatgt 23880
atgaatagta acccacacat atgcacgcat ctgtagctat ttctgtatct attatgtgta 23940
ctaaatggaa ctataattcg aatgctttgt gacaggcact gacccggtgc tttatgtatg 24000
ttatcttgct tagtccttga gtaggaggaa atattggcac ccctacttta cagttgagaa 24060
aggagaggtt gtgtcttttt tttttttttt tttttttttg agacagagtc ttgctctgtc 24120
tcccaggctg gagtgcagtg gcgcgatctt ggcccacagc aagctccgcc tcacggttca 24180
agggattctc ctggctcagc ctcccgagta gctgggactt caggtgcccg ccaccatgcc 24240
tggctaattt ttcatatttt tagtagagac agagtttcac cgtgttagcc aggatggtct 24300
caatctcctg acctcgtgat ccactcgcct tggcctccca aagtgctggg attacaggcg 24360
tgagccactg tgctcggcct gagaggttgc gtcttatctt aaatcacata tgcagtgaag 24420
tcctttgtca cttccaaaca ctgctcctga ccattgttct gtacctcatg gctgcctttg 24480
catgtgtaat tggcaagtcc tagctataag acagcatgta atgattaagg aggattggaa 24540
acctagtcct tgtatcaccc tttttccttc agtaactaaa atatcttttt tttttttttt 24600
gagacggagt ctcgctctgt cacccaggct ggagtgtagt ggtgcaatct cagctcactg 24660
caagctccgc ctcctgggtt catgtcgttc tcctgcctca gcctctcgag tagctgggac 24720
tacaggtgcc caccaccatg cctggctaat ttttttgtat ttttttagta gagacggggt 24780
ttcaccgtgt tagccaggat ggtctcgatc ccttgacctc gtgatccacc cgcctcggcc 24840
tccccaagtg ctaggattac aggtgtgagc caccgcgcct ggcctaaaat accttatgtt 24900
attaattttg tattttttat aacatattct ttagatggca aaatgagtct taatatttct 24960
gtcctttaag ttaaaaatgg tcaaaatatg ctttcagaat tacttctcta tattcattag 25020
taatttttag tataatttag cctgggcttg ccttaccata ttctgatggt gtagtttgag 25080
tattaagaaa atagaattta tttattgatt tatatttatt tatttttgag acaggtctca 25140
ctatgtttgc ccaggctggc cccaagctcc tgggctcaag tggtccttct acctcagcct 25200
cccaagtagc tgggactaca gttgtgtgcc tggctaagaa aataccctaa ggaaaaaaaa 25260
aaaaaaaagg ctgggtgtgt tggctcatgc ctgctgtaat cccagcactt tggaggccga 25320
ggtgagagag ggtgggaggg tcgcttgagc ccagtagttt gagaccagtg ttggcaacat 25380
agtgagaccc tggccctgca aaaatattta aaaagtagcc aggtgtgttg gtctttgcct 25440
gtagtcccag ttacttgaga ggatgaggtg gcaggatcgc ttgagcctga gaggtagagg 25500
ctgcagtgag ctgttctgtg cacagcactt cagcctgggc aacagagtga gatcctatct 25560
caaaaaaaaa aaggccctga gataatttcc taaaacataa ggaaaatcag atattgactt 25620
tttctatgtc atattaaata tctaatacat tttaattaaa ttttaatcca ttagcagttt 25680
gtgtttggtc ttgtaacagt acttgtctgt ataaaataac agtcctggta tgtttataca 25740
agaatattag gttttgtttt acacacctaa ttttgtttct tatttgggaa tagttaaaaa 25800
gagaaaataa ggaagtaatt acttgaatgg gaattttttt tttttttttg ctaatgtagc 25860
atagtctgtt gcttttatat attatatgta tttgtattaa ttttataaac tattgattgt 25920
tcctacttta gaaattcata gttgttaatt ccagcctggc ttgcatgcag cagactgtgg 25980
gaatactttc ttcactagtc taggtgctat aggcccagga aatataaaca gaattagctt 26040
tgataagaca tttaagaagt aaatctctac agattttata tttaaagatt tgatatgaat 26100
gacttctgga ttacctatgc cagtagtcac tttcattact atggaataat gagaatgtat 26160
aaagtttatt atggcttact agttgataat gttttcgtta atcaaatgga aacctaaaat 26220
attaaatgta taacataaaa taggtataaa atatacctta tgtataatgt atagggagtt 26280
tattgaaaac agtatgcaga taaattatat caaaattagt tctcagtagt catagatatt 26340
tttatcattt ggggaagaaa gattatgtgg gtatgtattt gaaattgctg cttttaaata 26400
tgcaaattca ggatataatt attctgtgtt aatacttcat aaggccaggt gtggtggctc 26460
acgcccgtaa tcccagcact taggaggccg aggtgggcag atcacctgag atcaggagtt 26520
tgagaccagc ctggccaatg tggcaaaacc ccgtctctac taaaaataca aaaattagcc 26580
aggtgtggtg gcacacacct gtaatcccag ctactcggga ggctgaggca ggaggattgc 26640
ttgaacccag gaggcggagg ttgctgtgag ctgagattgc cccactgcat tccagcctgg 26700
gtgacagagt gagacactgt ctcaaaaaaa caaacaaaaa caacagcttc ataaagcgaa 26760
ctgtcagagc agtttgctgt tgttcattgc ctcttttcaa accctttttt attttgaaga 26820
aaaaggtggc tcataacttt tttgttacgt agtctcactc cgctgcccag gctggagtgc 26880
agtagcacca tctcggctta ctgcaacctc tgccttctgg gttcaaatga ttctcctgcc 26940
tcggcctccg gagtagctgg gattaccggt gtgcgatacc atgcctgact gattcttgta 27000
tttttagtag cgatgggggt tttgccatgt tggccagact ggtctctaac tcctggcctc 27060
aagcggtctg cccaccttgg cctcccaaag cgctgggatt acaggcatga gccaccgtgc 27120
tcggccagct tttaaatgat tctcctgcct cagcctcctg agtagctggg attacaggtg 27180
tgcggtacca tgcctgacta atttttgtat ttttagtaac gatggggttt tgccatgttg 27240
gccagactgg tctaactcct ggcctcaagt ggtctgcccg ccttggcctc ccaaagtgct 27300
gggattacag gcatgagcca ctgtgtttgg ccaactttta aataaactat tacaaaatcg 27360
tgcctgcttg tctctcattc aaagaataaa acttactgag gagaaatgat gttagcccct 27420
aaaactcaga acaattactt gtatcgtggg tacatggata tgtgatataa ttagattagt 27480
atcttgggaa gcaattaaac tttccctgtg agcaccatgg ctttttaaag aaatgaatgt 27540
gttctccaag taggcaattt ttatagatta aacttttttt ccagtcatcc tttgtttatt 27600
gtttttggat tatttctttg ggagatgttg gtaaattgtt ttcagtgtgc tcattggtgg 27660
caaatcttca tatcttgaga atacatttag tattagaaaa tggccacagg tcatttgagg 27720
ccaaatctgg tgaataaaat atgtgctatg atgttgcaca atgctgttct agttgagaat 27780
aaattttgtc ttaagtgatt ctgaaatcag ttcttaaaga gttctgaaag agtagctgtg 27840
attcttacac gtttttggtc tcttcagaac ctcacaatcc ttacacaatt ttgttaagga 27900
attcgtaacc actccctagc ctgatagtgg aataaaggtt taggcttcca aggtgactgc 27960
tttgggaaca gccctcattt agatacagct gtgttagata cagctgtgtt atgtttagtt 28020
tgcagccatg cctggtgtga atgaatattg tatgtgtttt ttggttagtt ggaaaaaaaa 28080
gtccatccaa agaccattga ctgaggctgt ggttcataaa cactgaagag taatggtgtt 28140
atgtgtacga aatatttgtg gacatgctta cctaagtcag aatagagtgt ttagcctggg 28200
tgcccacatt ttcagagcag ttatttaaaa attgctgagc tgttaacatt cttgatagtt 28260
tctttgtata tgtgtttata gtttattcat ttggcaaaca ttctctgaac atgatatatg 28320
ttacagagta gagtgctaaa gaggtgtaaa gatatgaaat tgaaccagcc atggattcca 28380
gcagacaaga agtttattct tttttggcaa aggaaaggcc ttcatattct gtgttaaatc 28440
ctagaagttg ttttaggaaa tacttaagct cataatgtat tctttcactt tttgtaatat 28500
gggaggtagg acctgcttta tttttttatt tttatttatt tttattattt tccgagatgg 28560
agtcttgctc tgttgcccag gctggagtgc agtggcacga tctgggctta ctgtatcctc 28620
cgcctcctga gttcaagcaa ttctcctgcc tcagcctcct gagtagctgg gattacaggc 28680
atgcaccacc acgctcagct aatttttgaa tttttagtgg agacggggtt tcaccttgtt 28740
ggtcaggctg gtctcaaact ccttacctag tgatctaccc atcttggcct cccaaagtgc 28800
tgggattaca ggtgtgagcc actgtgccca gccagagttg tttttttttt tttttgagac 28860
ggagtcctgc tcttttgccc aggccagagt gcagtggcgc tatcttagct cgctgcaagc 28920
tccgccttcc aggttcacac cattctcctg cctcagcctc ccgagtagct gggactacag 28980
gcacccgcca ccacgcccgg ctaatttttt gtatttttag tagagacggg gtttcactgt 29040
gttagccagg atggtctcga tctcttgacc tcgtgatccg cccgcctcgg cctcccaaag 29100
tgctgggatt acaggcatga gccactgcgc ccggccccag ccagagtttt aaaatatgta 29160
acccttatta gaagcctttc tcgttttttt ttggtggaaa gaagggggta tatatcccac 29220
tgcagtctat accacaggaa catggtcagt ctgttaattt cagtgctgag ttgggatttg 29280
aaagtaagct gtttcttggc cgggtgcgat cgctgtcgcc tgtaatccct gcactttggg 29340
cggcctaggc aggtggatct cctgaggtca ggagttcgag accagcctgg ccaacatggt 29400
gaagccccgt ctttactaaa aatacaaaaa ttagcctggt atgatagtgc atgcctgtaa 29460
tcccagctac taggaaggcc gaggcaggag aatagcttga acctggaggg cagaggctgc 29520
agtgagctga gattgtggca ctgcactcca gcttgggtaa caagactgaa actccgcctc 29580
aaaaaaaaaa agtaagctgt ttcttcagtt atagatagca gtgtcagcag agtgctacct 29640
ggtccattcc tacctcctta atcataagta taaagtaatt gatccagtgg aacctcaata 29700
ggatattttt ggaaattcta gtggattcaa tgaaagaatc atcaactatc ttagtatagg 29760
gctgttaact tatactttct acacataact ctttaatcta gctttcttgc tcctttacta 29820
tcaccatgat tatttacatt tatatagtgt cttacagttt gtaacataca tttagctctg 29880
ctaattttgt atagcttgta cctgaagata aaggaaaaac aaaatgttaa cttaggcctt 29940
acagaaatta tttaaaaaca tcctgacata ggccaggtgt ggtgactcac acctgtaatc 30000
ccagcacttt gggaggctga ggcgggcaga tcatgaggtc aggagatcga ggccatcctg 30060
gctaacacgg tgaaatcctg tctctactaa aagtacaaaa aaattagccg ggcatggtgg 30120
tgggcgcctg tagtcccacc tactcgggag gctgaggcag gagaatggcg tgaacctggg 30180
aggcggagct tgcagtgagc tgagatcatg ccactgtact ccagcctggg caacagaaag 30240
aacgagtctc catctcaaaa aaaaaaaata aataaataaa taaataaata aataaataaa 30300
tgaaaaataa ataaatacat aaataaataa aaatcctgac attaaggttt ctgacttaaa 30360
attatatgtg taagtaataa gtatttacag tgaagtgttt agttatatat tgaatagaac 30420
ttttaaaaaa tgtaacccag ggttttcttg ccaaaattct ttattctgta gccaaagatg 30480
atgtctgttg ttaatatctt aaggttctga agtctacctg gaattatagt gcaagtaaat 30540
tgtagtctga tcatgtgtag tatctgtaat gcaaggtgtg accatcagct tatagatata 30600
gttgaatctt agcattattc taaggcttgg aagttaaaca tagaattata tacagagaaa 30660
caaagccttt catacattaa aatagaaact atttctgctg atctgtaaaa tgtccttctt 30720
gtggatattt tcagataatt ttccaagctt ttactatata tatgtataca tatgtatata 30780
tatgtgaata catatatatg ttttatgtac tgcaaaactg tagtctcaag atcattattt 30840
aaaagtacat ccttaacatg gctgggcacg gtggctcatg cctctaatcc cagcactttg 30900
ggaggctgag gcaggaggat cgcctgaggt caggagtttg agaccagcct ggccaacatg 30960
gtgaaacccc atctctacta gaaatacaaa aattagccca gcgtggtggt gcatgcctgt 31020
aattccagct actcaggagg ttgaggcatg agaattgctt gaacctggga ggcggaggtt 31080
ccagtgagcg gtgatcacac cactgcactc cagcttgggt gacagagtga gtgagactcc 31140
gtcttaaaca aacaaacaaa caaacacatc aaacaaacaa acatccttaa cttgccagta 31200
attcactctt acttttcttc ctgtggaaat gtgcttatgt taggttattt gcatgaactg 31260
agattattaa gttctaatat ttttctgcac attttggcca ctattatttc atgtagaaag 31320
ttgagagaat tttattcaaa gctgcctttg tgtgaggatc tgatagcata gaggtagtag 31380
aaatagacat tttttaaggg gtaactccca acaaaataaa agcgaagtac cagttgtggc 31440
agggatgaca gtggttattg gatcaccgag ctgtattctt tctatcagct ctctccaagg 31500
gactcagctt tctaggaggg ggagatggaa atcttaaaca ggtttgatag cttccctacc 31560
ttttatctgc ccagacaaca aaaataaaac agctcacagt gttaggattt ggggtgataa 31620
attaatttgg agatatctaa atgtaaaatg agatttctgg gcacatggca gtgcggtctg 31680
tatgataaac aactggggtt gaaacagtaa gtgcatgaac tagacagact ctttttgacc 31740
agaccttgtg ttataggtga atgaaatttg ggaatcctat acttggaatt ctggaatggc 31800
tagcctgggc caggaaaaat cactttgtaa tctaagaagg aattgagtta agaagatttg 31860
ttcatcacac cacatttcca tgttgttgag gaaaatagat tggcagtggt gacaaggtca 31920
aggatgtgtt gttcgaagtt gtagaagata agcagcaatt taaaggcgtt acaatacatt 31980
tttaagagtg gaaattgcat tgaagagtgg aagaaaagtc gacataagag aattagggca 32040
tgttgtttgc ccaggctggt cttgaactcc tgagctcaac caatccacct gcctcggcct 32100
cccaaagtgc tgggattaca ggcgtgagcc accgtggctg gcccttaata tgttttaaaa 32160
ggatgtttaa tactcttcat tactgtgtta tgcaatagtt gcatgatgat ttttctgatc 32220
tctacattct attttttttc tttttaaaat ggagcacaga agtacttagt ttctgatgtt 32280
tttccatctt ggcccagtaa tttccttttt ttttgttatt ttatgaagca caaaactaaa 32340
tatttttgaa ccaagagact actgtgtggt attaattttg attttgtact aaattcatgt 32400
tatttacaca aatcaaatag tgtcaacctt aaaatttttt taagcaatat ttttcttatg 32460
tttgagagtt aacatgtatg gttgattaga atcttcacct tctaaaggga ttaaggaaaa 32520
acctcgttca ctgttgtgaa ttctaaaatg ttttggttcc aagtcctagt ttttcttacc 32580
ctggtattat gtccaaaatg aatgcccact aaaagttagt aactctcatg gcaattaaag 32640
tattttgata atgtttataa gttcttgttc tcaattttgc cactagtaaa gatgtgggag 32700
aagttaatct ttttttgttg tctttcttgt gaacttaaag atcatttttc atcctgttca 32760
acctttggcc tgaccttttc aggtcactaa atgtctggtt tgcacttctt gtagcatgtg 32820
agaaataata cttcaaaatt caagtgtaat tgtattaatt tttgtgaata aactgctatg 32880
gagtgctgaa gtgtgttaag atgcctagtc ttttcagaat taccggcatt atggtattga 32940
acttaaaata ttttatgttt ataatatttt agaattttca tgagtcagat aaacatatac 33000
ccagattttc agaaacttga catttgctta attgcttaat aattcacatc aggcttagag 33060
atttgtgaaa tatttttggg ataatttatt tagaaatagc gtgtgtttaa atattaattt 33120
tcttttttaa gaaaatgggc agatgataaa ttaccatgaa agcttcattt aaaaatctgt 33180
tgttagtatt gtcttcttta attttgggga gaaaaaactt ctgagatgtt aacaattgaa 33240
catgtagtgt tggctctttt tggccttcaa tttaaaattg tcatgtgttt ggattaaaga 33300
catgatgtat ttttagctat tgggcaagag atggcaatat tgcattacat gatcactgca 33360
gttacactat tataaatgaa agtgaatttc atgttacaaa tgttcttcta atgcaattat 33420
taggcgtcat taaattagtt tacactttat ttcttctgat tgatttttta aaaaactttt 33480
tagcagtatt tatataaaaa ttggacaaat tgaccctttc agaggcagta tgaccctttc 33540
agaggcagta tgatcttgtg cagatacccg tctgcaatat gtgtgctgaa cagatgtgaa 33600
aattatttag tcactggctt actgcttgac accttcattg ctctgttgcc ctgactgtgc 33660
ttgtcctaga atctggttgt tgtcctcttt gtccatagga gcatatgaag gtgggagttt 33720
ttgaatttga ggacaaacat ttgttacctt taatactaca ttgctttcat actcactaaa 33780
aactaacctt tgcatagtat gcaacttgta tagcttatta atcttcaagt ctattaacat 33840
ataatatggt taaagaggct taataataaa acagaatgta gtaaactact gcatttaaaa 33900
atttcccacc aattcctact ttaccagcat atgtttgatg gttaaggggg aagtgatttg 33960
aatatctcag gatttgcaga gactttgctt ttacctcttc tttcattgta gcttggtcat 34020
tctcaaattt gagtttctaa ggacttttaa agtgttacgt atgctaatcc atgtatacat 34080
tatttttatg attgattaag gacttttttg ggaagagttg tggctataca tgatttttac 34140
ctaatctaat cattttagat tcaaactcct tgcataaaat gtagctctgc ttagcactgc 34200
tgccattagc ttatttctgc tggcttagag tgacgtctcg gctagttcct gtgttaagat 34260
aatttaactg gtttacattt tcttttttat gatgtgtgag ttctttctgt ctttctcatc 34320
tttacctttg acatgtcaga gacactgttg cttagttatt aagaacagac tgtcagccgg 34380
gcgcagtggc tcatgcttgt aatcccagca ctttgggagc ccgagagggg cggatcacct 34440
gaggtcagga gttcgagacc agcctggcca acatggcgaa accccatctc tactaaaaat 34500
aaaaaaatta gctgggcttg gtggtgcgtg cctgtaattc cagctactga ggaggctgag 34560
gcatgagaat cgcttgaacc caggaagtgg agattgcagt gagctgagat cacaacactg 34620
cactccagcc tgggcgacag agcaagacca tctcaaaaaa gaaaaaaaaa aaaaaaaaaa 34680
aaaaaagaac agactgtagc cagctcttaa tattaagcca gactgtaaaa ccaactacca 34740
ccatttatta gctatataac tttgataata tctctgtgtc ttagcatcct tattaaatga 34800
agatggcagt ggtagtcctt tctgaagatt aaatgaatca gtatatgtaa agtggtcaac 34860
tttgtatttt agtattataa taattattag atatgatacc tggcacaaag tagtaattca 34920
ggtgtttttt tgtgtgcatt agtgaacaag taaaaacaag tatatttctt gaatataatg 34980
tatagaagta attttattct ttttatcttt aagttagaag aaaaattaaa gtatattcca 35040
aaggttagat aaatgcagtg tgtatgggaa tcccttgagt ttttcagtaa aattctattt 35100
gggtggctgt caagtccagt ctgcatagtc ttgacatgtc ttggataaga tccttatact 35160
atcacttttc ctgtcatgga cactacaaat tatattgaat cagaaatcta atttaatcca 35220
ttcttattca attggaagca gtaattgctt tcaatttaat gggattggat tcagtattag 35280
aaattttgat gttccttcaa acttaataca gtatggttat atatatttcc atttatttgt 35340
gtgtgttcct gtgccctcgc agagagatta tcttggagta ttacttgtat tcacttgagt 35400
tatttgcaac ggttggtaaa gaggcatatt gtaagggtgg gaatgagagt ggcattctct 35460
ttgttttttt tcttttctac ccatggaata atgaagagag aaataataag ggccttcaaa 35520
atgggatttc catccactca gaaataagga aaggaaattt agagtctctg gagaagatct 35580
ggaatgctat ttactcttga gggtagacag catccagccc tactaaagat gaaaaaagga 35640
gagcctgggg atgaaattaa atccatcaaa aaattcactg ttggtcaggc ctggtggctc 35700
acgcctgtaa tcccaggctg aggtgggcgg atcacttgag gttaggagtt ccagaagagc 35760
ctgaccaaca tggtgaaacc tcgtccctac tgaaaataca aaaaaaatta atcggctatg 35820
gtggtgggcg cctgtaatcc cagctactcg ggaggctgag gcagaagaat tgctttgacc 35880
tggaaggtgg aggctgcagt gagccaagat catgccacta cactccagcc tgggcaatag 35940
agtgagactc catcttaaaa aaaaaaaaaa attcattgtt agcatgttat ttcaatcttt 36000
cccggcaaat catacttaga gacagctaag tttatcaatt ctcatgtcct atacggtgct 36060
ttttcttgaa tatacggtag acaagaggta aggcctaatg ggtgaagtaa ctacatttct 36120
gagttagtgt ctaattgttt ttgatcacct agttccattt gacagaagat gtagtgctta 36180
tggctcatcc cattgactct ttctctgctt tcactccttc agcagtgttt tcagtagcct 36240
ttttggtgat cttattgact tgtctttttt ctgatgttct ttttaattga attgctatat 36300
gatataagat ggaatgattg tgttttaacc atgatcttca agtctgctgt gtaaagaact 36360
aatgattgca gtttgagtaa aattcttagt aacaagtgaa tcaaagataa agatagatca 36420
ttgagaatat tttttttctc tccatgagga agttcattat tagctagctt acaaaaatga 36480
aggaattgtg agagtgttct ggaataaatg ttatggctta ataaaactat tcagaaaatc 36540
actgaacaat acaaaagtgg aagacattga aaatggtata tggacacatc taagatcaaa 36600
attaagccag agaagcttta gggagagtat ttgaagctta tcatttccat ttgtaacggc 36660
tttttatatt gtggtgtaaa atcatgtgca agccataaac aagggctttt aaaaatattt 36720
ttgttaattt ttttttagtt ttatagagat ggtgtctcac tgtgttgccc agtctggtct 36780
tgaactcctg acctcaactg atcctcccat ctcaaattcc ctagtcactg ggattacagg 36840
catgagccac cacacctagc caacgagggc tttttttttt tctcattaac atagagatat 36900
gaagaatact atattggatt aaaagttttt gcatttgtga agtgtaagca gctgtgtgaa 36960
tgtagtcaat gtgtattctt catttaataa acataagtat cttttccttt ccaaattata 37020
caaaaaatac taaaagcaat caaacgattt ttacagaatc ttaactaata taaaaatttg 37080
ttcattgctt ataatacaga ataattatat aacttggctc attatctaag cttaatgaat 37140
taatgttgga atgtaaaaac gagcaatttt aagccagcga tggctcaatc atttttaact 37200
gagggatttt gggatcaatt actaaaatgt tctgagcctc agtgtcttta aaatgtaatg 37260
atgctctatt aatctcatga aattgaagtg aaaacaaatg tgaggtaata tgtttttgga 37320
aatatttata agtttaaaaa tagcctgctt ggtacttgtg cctgtaatgc tagctactca 37380
ggaggctgag acaggtgaat tacctgaggc caggaatttg agtctgtcct gtgcaacata 37440
gcaagacccc atctccaaaa aagctaatat atttataaag cttaaatgtt ataaaatgat 37500
aaactgatgg tttttgaatt ctttgctcta ctcacctttc atctgttaaa gatgtttttg 37560
tacaatttag ctcagttcct aaatatacca aaagatggca ctgtttacct atgtttaata 37620
ttttaatttg cattttttga tgaaaaagtg tgttgaatgt tttatctaca aatgttttaa 37680
attgaggtta tttttttaca tttaattttc tgttttgtaa atggatcttt aattcatgta 37740
aagtttacta ggaccaggtt tcatttttat tttgaaatgg tttggaaatt cagatattta 37800
acaatcattt tgtagatata aactcctgtg tgatccattg taattaatga taaaaccttg 37860
tattaatgtg acagctaatg tttcatggaa aacctagttg cttttgtata acaaaaacca 37920
ttaaattttt taagcctctg aaaacagtcc agtgattaaa acatgacagc cacaaataaa 37980
ttatttatgt ggcttttagg tcacactgcc caggtttatt cattttagaa agttatttta 38040
tcttaaaagt acctttgaaa atgtagcaaa acctttcatt gcttgaattt cctactcaca 38100
gttgtgtgat gagaggagaa tctaggtcca ggtcctgcga agagcttctc tgggtgagaa 38160
atgcaaaaat tagataatat gaatttgact tgaagtgagg gttatttata tagcaggatg 38220
tggtgaatac acttcccata cagttagacg gaaacctcct gattgttcat ttatacagcc 38280
tattacatcc aatgtagttt atgacctaga aagagaatac aagtaggata tttttttatc 38340
ccatcaaagc tatctatata tatatatatt tttaaatgaa cagcatttgt cagtgatttt 38400
tacaggggtg aaaaagcaca agacagttcc attactatta taaaatgttt tacttttgtt 38460
gtgggggtaa gtttagaagt ccaagatata ataatttcag atgtgcttct actgtttcct 38520
ttacttaaaa gtagggacta ttttgaggca atataatttg tatgtttcag tttttatttc 38580
tctctgaaaa tcacttttta gggtaccatg ccttggaaag aaagtttaca tttttttccg 38640
ttaaaaaccc atcctcctaa ctctgaaatg actgtctcag ttactaaatg tatatagctt 38700
gggggaagtg aaggcacagt tggcagttag aggtttgttt ttatgttcat gggagtatat 38760
gtgcttatat ttaaatgttc actcttctag cagggtttgt tgatgttagt aatatgctac 38820
atatgcgatt tgtctgaatt gtttaaaaat gcataattac aggaggattt gattattcag 38880
caacagttcc agtagctaaa gactatcata tagatatttt cttccagtat ttgacagtgg 38940
ttctgtgagc tatgagtagc ataaaaaata aaaagaagat actgtgactc tttaataccc 39000
tagaggccgt agtcttagga tattcttact gtagagatga aaaaacaggg cttcattaac 39060
aaactgaact gctatttcac ctcttttagt agatgtgtaa ttctgtgggt ttggtaaata 39120
acaaaggctt ttttcctaaa tgatttaatg ctttcagatt tttgtgggaa aattagaaaa 39180
agcaaaaatg cactcagtga tttttttttt aaaccccttt gtttctggac aaatggacaa 39240
agatagttaa gtgtcccaaa aatggtttta acaggcttcc ctctacaacc ccatacatac 39300
cctggctgtc cataattcca attttgggta ggtagtaaaa tgtgatatta gactacgagt 39360
attttccctc caaataatac ttgtgattaa aagaaatgca atcaaatagc attatatatt 39420
cgttatgaaa gaaagatctg tcatgcagat tttgcctggt tcctttagat gccttgtaat 39480
aatgtgctaa tttatgctga tgatcttgcc tcatgaccct tctgtgtggc ctagatatgt 39540
taatatgtta tagcttttta atttcgatcc tctgtgttgg ttgaatatgc ttgttagtcc 39600
atagtgccac tcactagtct gtgtcaagaa ctgaaaaaga acaagaaaaa tgtatctcag 39660
aatcaatttc tttaagcatg tatcagtaat ggaggaaatt ataaaatata cataatttta 39720
ataggacttt atgtctgaac attcattgaa aatagtaagc actctaagta ctgtaagctt 39780
ttaattagga aagagagttg ggtcattggt tttattaagc aagggcttat taacatcttt 39840
tgattttttt tcttgatttt tgtttaatat cagtgagaaa atattacaag aatgaaaact 39900
agacatcact ggcagttttt actttattgc atccattttt ctgttcccta aaccttgagg 39960
gttatgcatc taaagattcc catggtagga aaatactgta tgtggttcat aaattcatga 40020
ccttaaggta gcagagacca aggcctaaaa tagatgggag aactcaactg tttaaaaaaa 40080
tttgttaaaa taaaaattcc atagaaggaa aacaatttat gtaccttaaa tataattcat 40140
ctttattatc tttcctcatc tacagaagct tctccttatt acttatttgt gttctttcta 40200
cagagaatat actaggaggg gtatttttcc ttatttttag ctcttgatct gaaagtgtaa 40260
tgttcttttc gctttgtctt gaaattgttg cctccccatt tttgggtctc atctggatct 40320
gaaagacatt gactgactca aatatccgaa aaacttggta actttcaaat tggtatacgt 40380
ggcataatta agaatatgtt ggtgcaaaag taatcgtggt ctttgccatt gaaagtaatg 40440
gcaaagacca cgattacttt tgcaccagcc tgtatttcac aaacgtctct gtgcctgtga 40500
ttacttctct ctaaaatggt ttatggagta gttgttatga ttaataaatg gttgataaat 40560
actagctatt atggtattaa gcattaataa ggaaaattca ctattacatg taaatgtttt 40620
gagtccctga aattaactgg caacttaatg aaatttgatt taagcacaca tttagtccat 40680
ttcaatctat ttaaacgtgt ttctctcttg cacatctttt tctgatggag agcgtgagtt 40740
aggaaatgag ccaaaatagg atatataatg gatagcctct tttggtagcc agtcagacag 40800
aatggggtga tgtcttcata gaaggaatat ggaattagga gttggaagat ttggggtctt 40860
gctccaaaga tcactttgat tgttggtgaa cttatttatc tagtctcagc tttttaatta 40920
attaattaat taattatttt aatttagaga cgaggtttta ctttgtggcc caggctggag 40980
tgcagtggca tgatcatagc tcactgctac tttgaattcc caggctcaag agatcctcct 41040
gcctcagcct cctgagtagc tgggactaca ggtgtgcacc accatgccca gcaaattttt 41100
ttttttttga gacggagtct ccctctgtcg cccaggctgg agtgcagtgg cgcgatctcg 41160
gctcactgca agctcctcct cccgggttca cgccattctc tcacctcagc ctcctaagta 41220
gctgggacca caggcgcctg ccaccacgcc cggctaattt ttttgtactt ttagtagaga 41280
cgggggtttc accgtggtgg ccaggatggt ctccgtctcc tgacctcgtg atccgcctgc 41340
ctctgcctcc caaagcgctg ggattacagg cgtgagccac cgtgcccagc acccagctaa 41400
tttttaaatt ttttgtagag acggagtctt gccattttgc ccaggctctc ctccaactcc 41460
tggcctcaag ggatcctccc acttcagcct cctaaagtgt tgggattcca ggcgtaagcc 41520
actgtgcctg gcctattctt agcttttaaa ttctcgtcta tgaaataacg catttgactg 41580
ttccaaaagt gtgggggaca ggtcatcttc tttcacatgc attgtcttac tttgtaaggt 41640
aaataaagta ctatattaag aaatgtgggc ctggcacggt ggctcacgcc tgtaatccca 41700
gcactttggg aggccgaggc gggcggatca gaggtcagga gattgagacc atcctggcta 41760
acacggtaaa accccgtctc tactaacaat acaaaaaaaa ttagccgggc gtggtggcgg 41820
gcgcctgtag tcccagctgc ttggaaggct gaggcaggag aatggcgtga acctgggagg 41880
cggagcttgc agtgagccga gatcgcgcca ctgcactcca gcctgggcga cagagcgaga 41940
ctccgcctca aaaaaaaaaa aaaaacaaaa aaacaaaaaa acaaagaaat gtgaggtgtg 42000
aaatccttct tgaacatttt taacttcact cctccctatg ctgccactct gccccctcat 42060
cagactcttc cttaacacac tgctgtgacc cagattgtct ttatttcttc ttgtagcact 42120
tgttagactc tattgcaatt atttatgtat ttgtctcatt agactgatct tgagtacaga 42180
gatggccctt tattcttgta ttcaaagtac ctaccaccat atttggctta taataagcct 42240
cagtgtttgt tgaatgaatt tatatcaata tccatgccat tcatgagaaa caaaacttat 42300
tatatagagc atagaggtgg tttctaattc gtgttgctct tatttttact gttagatgta 42360
aaacgttgaa tgatttgtta ccacttggca taattaaatt tagcattttt ctagtttaag 42420
tagttcagtt tttgagacac ttttatgagc ctcactatat taatggcctt attggtgctt 42480
tacagatttt gtgattcatg ggcactgtaa gtataaattt catgcttaca gcaattgaat 42540
aaaataggca cattgttctt tgttagtttc ttcagttggt tcttttaatt ttcctgttct 42600
tactggtgat tccttgaggc atctggccag tgttgagtat ttcttcctga ctcatcatcc 42660
cttctgccac ctgccaaagg aatgctttta ctttggtcaa gagcatttcg agtgagttag 42720
atttcattat tggtgtaggc tatggaccac ctgataagtc taagcatgtc atactaatta 42780
gcattgactg ccagccacca taatgcttaa ttcaggtgct atgctgtttt aattctaaag 42840
gtgttgtgaa gtatggaaaa atcctttacc tgaatatgaa actagctact aaatttggag 42900
ttctctccct acccctgcag cattttcctt tgtgtgaaat ttcagttgat tatattcaat 42960
ttacttttat atagtctttc agaatcagta gaattttttt ccttagatat atctaagtca 43020
ctcctttttg agatgattta gaaaagagga aattgaggat tggaagttaa aaagggagaa 43080
agatggctgg taggtaacat gtagaagttt taacctttca tctcaagaga gtgattcttc 43140
tgggctttaa tacgtgtatc tgagagctgg gaggaagctg tgttggaggg tgtctgggac 43200
ctggtgagac ctcagaaaat aagagtccat cataaagtaa gaaaggcatt gatgggactt 43260
aaaaatattt gactacagcg aaagcttttt atttatttat ttatttttat ttttaattaa 43320
ttaatttttg ttgttgttgt tgagacggag tctctgtcgc cagggtggag tgcagtggca 43380
tgatctcggt tcactgcaac ctctgcctct tgggttcaag cgattctcct gcctcagcct 43440
cccgagtagc tgggactaca ggcgtatgac accacgccca gctaattttt gtatttttag 43500
tagagacgga gtttcaccat gttggccagt atggtctcta tctcttgacc ccatgatcca 43560
tccacctcgg cctcccaaag tgctgggatt acaggggtga gccactgtgc cctgccaatt 43620
aatttatttt tttgagacga agtctcactc tgtcgcctag gctggagtgc agtggcgtga 43680
tctcagctca ctgcaacccc cgcctcctgg gttcaagcga ttctcttgcc ttggcctccc 43740
gagtagctag gactacaggc atctgccacc actgccagct aatttttgaa tttttagtag 43800
agacagagtt tcactgtgtt ggccaggctg gtctcaaatt cctgacctca ggtgattcaa 43860
ctgccttggc ttcccaaagt gctgggatta caggtgtgca ctgggtcagc tttttagaat 43920
gcattttatc tgctgcaagt taaaagaaat ttactagcct ttgaatgaaa ctgtagaagg 43980
aaaatcctga attcctagat atcaagggat ggttagagaa cccccaaaag tgccgtattg 44040
atcattacaa aaaaaagggt aaaatgtacc cttatagtct cttcatttag tgcaatatct 44100
ttattgtggt aagttataga ttattgatag gggcttctat agtttatttt tctacttttg 44160
gtctccttat cctatcttcc cactagatta aaaaattctc cccaaatata ttctgctgtg 44220
gggattttca gaaatgttag caccctcact ggggaggaat taccttctac ctgtatttct 44280
ggaaggggaa gcttatgtga atctctaaaa agagaattat attgatgtta cgaagtaagt 44340
actataggtg aaagagagaa taagaaaaaa ctcgatgtt taaaggagcat ctattgagtt 44400
actctgtgca aggctgacca actcaccttg tgatcttagt caagccatgt aatctccctg 44460
agcctcagtt tattcatata taaaataagc aagttgaact cctctgttcc ctggttcttt 44520
gtaaattcat tcattcttag atattcatca taagcctgtt gtgttatgta ttcagcagtt 44580
gaacaagaca gatgcagtct tatccttgta agaagacttc tctttcccct atctctgctg 44640
acctgtcctt ctccctgttc tcttatctag taattggttg gataatgagg aagtgaagca 44700
atgtgaaatg cttaattaca aatttgggga ctctgtctgc ttttatggat gaagtggatt 44760
gacattttgg ggggaaaagt accctgtaaa aacatagttt atatatatta aaaatgttat 44820
taaaatgaaa aagtgattta tcagttagtg gtaaattaat tggctgactt gggagtgaaa 44880
attatttgac ttgggagaca aaattactct ctagactgag ctagagagta attttgtcta 44940
aattgaggta tagcagatat aaaatggaaa agatttatct ttgatgttga gtaattttaa 45000
aaaatgcatt tgagcttctt gggaatagtt acccatcttg atgtatttgc tgaagatcag 45060
atgtaatttt aaaaaaaatt tttggtagaa aaatgtcttt ggtataagtc aggcctatta 45120
tcttgagatt tatgccttta ttctggaaaa ttgaaatttt tatctcataa aagaggaaaa 45180
taatttgaat ggagttaata aaaatgaaag gagtaaaatt tcacttagaa atacagtgag 45240
gcatccaaaa tgaagaaaaa tcccttattt ggcttctaga tctaaaatga tattctaatc 45300
tatgcaggtt ggttcaagag cactttaccc ctgttggagg cagccaggtt agctttattg 45360
aatgtaaatc tctgacttct cccaagatac ccatgaaagc aactaaattc attggttatt 45420
aggcagcaag agtaagttca attttctcta cacttgtgac aattagaggt tggttctagc 45480
ctgtctaatg cttaagtttc aaggctacct gtatttgaga gctgttttgg ggagataacc 45540
ttgtaacaca tacattttaa tgagtacatt acttttacca cctgctgaag tcgacttgga 45600
gtgactactg acctgaagtg aatgcctgtg ttcaatatta atatggcatg tctggatcca 45660
gacagcaatc tttcattttg acatgcacat agtcaaagta aattaatgtt taaaacccaa 45720
acactttaag ttcagccagt gtgtgcattc cataataatc cttcagaagc agtttacata 45780
gaaatgctga gtgctttctt atactttatt tatcatatgt attgggtcca cattgcagta 45840
cttataacga gatttatcca gtgctgtttt tgttccttgt tttgtggagg ctaaagaagt 45900
taatttattt caggatttag tctctgcata ttttaagcaa aattatactt aaaaatcttc 45960
atgtgcctgg caagatagta gtctaatttc agttatatta tttttagatg gaaaaaaaaa 46020
atcaagctaa ttgcatactt tatttctgct caaatgatca ccaatttttg aaagtatagt 46080
agtagtttaa aaaaagaaaa aaactaggcc cagatatagt gtatgtttgt gactacatgt 46140
aacaaggata ttgctgattt tccttttctc tcatacatac cattttctag tcttgctttt 46200
attggttaga aaacattcat ggcagatgta gtaatgggga aattatatta acatttaagt 46260
attaaattag cttgtagcca gagtctcctt ctaatatgat gacatttagc aagttcagga 46320
ttttaaggac catgttgcgc aactcattgt tacacatttc ctgtttgcct tttttgaaaa 46380
agtatctgag atctgttttt tcagattgga aatttccatt gtggatttca atgttgttgt 46440
tgtcatttta ccttgctggt cagtgtttgt tatttatgta ttaagaatat gtagtatagt 46500
gaccataatg agaatagtag gtgattggag ttgtatgcaa atgacagttt tcataacact 46560
tttgcagttt aatttcaaca aaaacctcaa gttatattta ttagcaaaca tatttcagct 46620
aaggagtttt ttatgctcca aaaagatggc agatggaaat gaacctaata tgtgttcaaa 46680
gaaagtatgc tgcatttatt tcaaattgta tctgatacat cagcatgatg atttatatgt 46740
tgtcttagaa ttaaagtaga tgccatactt ttactgtaaa agtcaaaatt agatttcttc 46800
tatatttgac agaatttatt ttctgttcat aaataaatat agtatttggt ttaggatgta 46860
gaacatcaaa taacaactgt tagattatgc ttaatatatt tatcattttc tgaattttgg 46920
atgttcttaa catcagaaaa atatgtcctt tacccataca gtgactttag attggatttg 46980
gaagcattgt acagagaaga cctattgttc ctattgaata aggatgccgt acagagatga 47040
gctttttgac ccactgctct tccagtagta atggatttgc actaatttga gcagagtatg 47100
ccacttggtc ctgtttttca gttgtcctgt gtgatggctg gctagcaaaa tcaaacatct 47160
gcattttgac tgtggaaggc agtataacta gctggctcat actggttcaa atttgcaaca 47220
ctgatatggt tagtattatg gtgtcactac ctgaattaag tagttgtggc taaattgttt 47280
atattatatg aacatattcg ttattattct gcatggagaa tttttgtcct catggattgt 47340
ttcataaagt gttttattag cttggattaa ttggaaaata agctttttaa tatggacctc 47400
tcccctagtc agagactttg caaatgattt ctacaaaaat aaactataaa atagatgata 47460
taatggggcc tattattcct gatttgattt taatttgctt ccttttataa tttttccaca 47520
ataaaacaga gcctctcaga aagtaaaagg atgtaatcct agctcagatg gtatagggtc 47580
cttatgtctt gagctattat gatggaaggt acccctatac ctccactgat agaaatatga 47640
tgggcagaca ttttctttgg tgaaaagtag ttcatagttt actgtgatat ccactccttg 47700
gacttacttt agaggactgt ttactgaggg caaaatatgt gcatgattac atataatcaa 47760
ggttaatttt tatcaagggt caccatgttt ttgctatggt ctgacaatca tgacctacat 47820
taaaagagcc ctgtgggtct gtctggtgta tttttgcttg atgtctgggt agtttatttc 47880
agatgcttgt cagtttcatt atatattttt gtttggggct cttgctggat tttcaggtac 47940
accataaaaa cagttctctt tgcagttaga ttgtcatgaa atagcttctg ttttatggta 48000
tgaaaacact ctttaatctt gaagggtgag taattttggg aacattttcc atttggtggt 48060
gtgttatgtt caaatggtag gttattttga aataggatag actttgcagc taagattcca 48120
gatttagttc agttaattca ttctttagtg agtttttctt gggaaaatat tttggaattt 48180
tcatttcaaa tgcatcctcc tcatttaaga aaaatccaga tgagtagttt atttcacaat 48240
ataataacta aataggattt ataaagcaga aatttataaa tcctttcttt tgctgggaca 48300
aaggcaatgg tgaaaattaa aaaaaaataa agttaggaca tttaggtaac tcaaagacag 48360
aacttaatgg ctgttaagat tggagtattt ggttttgtag tttttaaacc atatttaatt 48420
ttggccatat ctatcttatt tagtagttta aaggataaac agttcaaaca gtaagaaaaa 48480
agcagtttgt gttttgtcag ttttcaactt tagatatcag taaatcattg ctgtagcttt 48540
ctttatcttt tgtttgcaca aaaaacctgt tagaattatg tctgcgggga ccatctgctg 48600
tggttcccag tcttgtcagt gtgataaggg ataagctgta taaacagggg caccacaaaa 48660
aaaaaaaaaa aaaaaaagaa agcaaactgc ttttagaagg gaaaaagtat ccatttaatt 48720
ttataacata gccacagaaa ttcatatgat ttttctcata atctatcaga attgaggttg 48780
taatatattt tttcagaaac cacaggtcca agttatttct atggtttatc ttcacattaa 48840
tattatatct ttttgattac agggtttcta ttttggaggt catagctttt aaaagttctt 48900
cgaaaatatt tcagtatagc acttgacatt gcaatatttt tcacattgtc tgcttagttt 48960
ctggatcttt ttttccctag ccattaattt ttctttcagt tgaatcctca gaaatcatcc 49020
ttttcctgtt tcatttgcaa ggaggtcatg atgcttcgta aggaacaagg cagtcatttc 49080
catggcaaca cactctatca aaacatcaca aatctgtcgt gccccttacc tttaaactct 49140
caaatgctaa gaggaggagg tgtgtaagga gcagcattgt tcttacagca taactatacg 49200
atcacaagga gagggaagtc aattaattga ctcaaaagta ccttaagttg acttagctat 49260
agacatatat atgtaaggaa tttatctttt atattgtatt aaacagtgtt ttgaggatgg 49320
aaaaatgaac attccaaaac tctgatttaa agatcttatt tagtgtctgt gagaactgta 49380
aaagaagaat aaatagctga gttatgtcat acatgtaaac atgttacaca gttactccag 49440
tcatgagaag tctacctttg cctggaggcc ccagcacatg tctctgtcag tcattttggc 49500
ttaagctagg atattttcag catgatagtg acacctatct tttaaacaag ttccagaatt 49560
ttgtcaattt tctttttctt tttaaccatt aagaggaggt gttagcaaga tggcgtgact 49620
cctggatatg gaataggaga acaattggtg tttcatcatt actgaaaatt ttatgctctt 49680
tgaaatcagg tatgaagtat aataaaaatc aacagtttta tgagattttg tggtaaagca 49740
attcttattt tattaacttc aagtcttctg cattttcagc atttcatttt attctgtagc 49800
atttttactc ctgggtaatg catttatttg tgtgacctgt gaagatggta aattttgcaa 49860
tgcttagcgc cttctgtttc cccatgttcc ttgtagcaaa ctctatgcta gtccttatca 49920
tagaacctac ctattgtact ttaggagtca gctcacagga ctgacacctc ccacttgact 49980
gtagccacct tggaggcagg agcttgcttt tctctgtgtg tggcgtggag caggtgttca 50040
ataatgtttg ttgagagaac agtggtcaga atgaatggaa ttagttgttt gtgataatgt 50100
ttattttctc tatatggcac aggtatatat atattttaat tttttactgt gtatgtctaa 50160
aatgaggttg aaatatgttc tccataccca ttttgtattt aacaattttt ttgtagtgta 50220
ttttgaaatc ttggtaggct atttcctttt gccttttcag gcctccatac actcctatta 50280
caagaggact tctgtctctg ttccacattt cccttcgttg ttggcgacct aaccttggtg 50340
accgcaagcg atcggttgtg agagaaagag ctgtaagtgg ggaaggcttt ccgagtctat 50400
aggtactacc tggtggcagt attaacacat tttattatga actatttgac agagaaaagg 50460
attaagagaa taaggaccag ttatatatca cctactcaga tttagaaatg aaacattact 50520
gatatagctg cagccctcca tgtagggggc gggggttagg tgggagtata tttttcattt 50580
tcactttgta cctttttgct ctacaaaagc ttcagacttc aaaagaaatc tttccttgct 50640
ctgtgctttc atagtactac cccaggccta agtgtattat tgaacctgcc agtgtattat 50700
aaccatctgt tcagatgtct gtcacctcta ccaggctgta cttcatcttt gtattttcag 50760
taccgtacat aatagctaga atggaatagg tgctcagtaa gtgcttgttg tgtgaatgac 50820
tgaatgaatg aataggtaca attcttttct caaaatacca tgttaatgta ggattctgtt 50880
tagcaaaact atttccttgt ctagcccata gttacagggc cttggtgctc tgatttgaac 50940
aaattgaatt aaacataaaa tcctgtagaa cagatgcatt tacaagtctc tgctcacaca 51000
gctgctggtc tatttaataa aaacattgct gttttgattg ggtattcata gcctttcttt 51060
ttgttaactc tattagtatc atggtataag cagactacag tctagggaag atgagtggaa 51120
gaatgagcca gtgatcagaa ctcagagaga caataggaga tggatgtcaa gaagatggat 51180
ggggaaaatg ttttagtctt aggtgtagtt acaataaaaa gaacaaatac atgtatttgt 51240
cttccctgga ctatatgtca taaatctctg actccatatt tcttatgcag ttattgtaac 51300
cttccttccc ctaaaccacc taagtatgta taacattcct actctaaatt acaaaaaatc 51360
gatactgatg catttgcttt ttaaattttt aaaaattcag gtataattta tatccaataa 51420
aatgcataga tcttaagcat tcagttgagt gaattttttt tcccatttat gcacctatgt 51480
aaacactacc ccaaacaagg taggggatat ttccatcacc ccagaaaaat ttttcctacc 51540
tctctcccat caaccctcct tttcccttcc cctcccctct gcagcagcca ctttctgatt 51600
ctgatttcac ttgttcttgg gagtcatatg aatggactca tagtatgtac ttttatgtct 51660
ggcttctttt attcaagata ctgtttttgg attcatccat gttgttgcat gtataaattc 51720
gatttgcttc tagcaacaga aatgtgtata ctagcaatca aatacaaatt tacttttacc 51780
ttaaagttga gagattgagt agtgcttttt taagaggatt gttagtagac ttccacagca 51840
actcacatat attgagtgcc agtccctacg ctcagtgctt tacacacatt atcttattta 51900
attgtgtttt gtgattgttt gtttagttgt ttacttaatt ctctacggca gacatgttcg 51960
gtgaaagcat tgacatctga tgaagaagtt gacttggtgt ttgacaaaag aaactgtctt 52020
gtgatataaa attctgattg ttttctcagg actaagtact aagcattgtt tatggcaggc 52080
atcgatgagc ttaaatcatg aaacaggagg aaaagtgtaa aaggacaatg gaagagagtt 52140
actgggtggg aaagaaagaa gtgcagtagc tagtttagat tcgtcttata caatctgtga 52200
tgaaactttt gagacagagt ctcgctctgt tgcccaggct ggagtgcagc agcgcaatct 52260
cggctcactg caacctctgc ctcctgggtt caagcgattc tcctggctca gccttccgag 52320
tagctggcat tacgggcctg caccaccatg cttggctaat ttttgtattt ttttggtaga 52380
gacagggttc aacttgttgc cctggctggt ctcgaactcc tgacctgaag tgatccacct 52440
gcttcagcct cccaaagcac tgggattaca gatgtgagcc actgagcccg gcaaagttgc 52500
ctttcttcat agaagaaagt tgcaaaatat acatgaatga aagtatatta catacaatac 52560
ggatgcctga aaaaaagcat atgtaaattg tgcttagcct ctcaaagatg cttgtaatca 52620
gtgcccttat ttttgttcag aattatatca acctagtttt gagtttttac atatagcttt 52680
tgttttacca gcacatatgt aatacctgtt atggtgccag ttcattgtcc taattgcttt 52740
atgtatatta attctcaaaa ctctgtgaag tgggaatttt ttctgttttt tagtccctgt 52800
tttccccatg aggatactga tgtaatacga agttaaagaa ctggaccaga gtcacatagg 52860
tagtgcaagt ggggctggaa ttctaacctg gctggtctga tttcagagcc cgtgctctta 52920
atcactgcac cgtgtgcctc agcctcccct ctccctaaca tgctcctcat tcgtccttga 52980
tcctagggat gatgctggtt gagtatttca aggtttgtaa agattgacag tatcaaaaca 53040
gatgtagcaa ctgggacttt gataatttct ataattcaca aaatagatct taaaattgca 53100
tagttctgtt ttttgtaggg gagggaagga tatgtaggaa gggatgattt ataatttgaa 53160
atctggcagc agtagagcac gtaccttaag cctcagcaaa gctaattaca taaccaattt 53220
tgtgcttatt caaggctgta tttatagtag attggtgttt ctgggctagt tggtcgtaga 53280
ccctgggagt gagagaccat agggacggtc tgtgaatgta cgtatgtttg aattaggcaa 53340
tggcaattgg aagtatttta ttaccacatg aggtcctggt actcagattg ggcacacctg 53400
cagtaggttg ttgacatttg tgaatctaac atttggggtt tcaattattt tcaagccccc 53460
agaaagtcca tgacattcag tagtttgtta tgaaaataat atcagtttca tagagttgtg 53520
gggttctgag aattaaataa aaataatgtg tgtaggcgtt agctgctgga aggctggaat 53580
acagattcta gtcatttggt gagtgagtga acctagcaga ggtcctcccg tgtctgcttg 53640
tgacattctg catttgggag tctcaggatg catcagtgac tgtgctgtcg gtgcactatt 53700
atggccactt ctcatccttg ctggactgtg agtcttctct agactcaggg cttcagagtt 53760
acttctgccc ctgtgtcctt catggaacacttacttttta cttctgtttg gtctttcaat 53820
ataaagttcc tttattttta attaccaaac ttccctctcc aggttataag taaggtggtt 53880
tttggccatc acctttctta gctatgggtt aaagcattct gggtactatt ttctcctttt 53940
tatcaccatg ccatagccct tgtgtctctc ccttcaactc atggttaggt tggtttcatt 54000
ggcactttac tttttttttt ttagatggag tttcgctttt gttgcccagg ctgaagtgca 54060
atggtgcgat ctcggcttac tgcaacctcc atctcctggg ttcaagcgat tctcctgcct 54120
catcctcctg agtaactggg attataggca tgcgccacca cgcccggcta attttgtatt 54180
tttagtagag atggggtttc ttcatgttgg tcaggctggt ttcgaactcc cgacctcagg 54240
tgatccgccc accttggcct cccaaagtgc tgggataaca ggcgtgatac ttttaatttt 54300
taaaccttaa aatacattat taatttttcc aagagaagcc ccgatatttt tgtagcttgg 54360
cctccagagc ctcagttatc cctgatgtag tagatcatct ctttgcttcc aactaaagtg 54420
gagcattgga tgttgtttgt taaggagtga ttttcccatt tactaactca taaaatttgc 54480
tgactgtgtt ttttggtaag aagtcttata atgctttcaa tttcataaga gtcattttca 54540
tagagtccat gcaatataca cttagagtgt aaaagatact gagttagatg ctgtaggggc 54600
tccagaaatg aagtcctgtc tttcattctt acaaagtgtt catcctttta agttccttgt 54660
taatgtttgg ggcaaactaa atataatttt ttggttagac tttaggtaaa tatttgaaga 54720
aaaattttat atgctatatt aaatttttat tttatcacaa gtttcattaa ctttcacttt 54780
taatgccatt ctttatgaaa tgtcagtgtt aaagtgaccc cagtagtgtg acaggcaaaa 54840
gggtcagtaa tgatggcttc tttagtagcc ttcagaactg agcagtttga gattaacagc 54900
caatatgaac tgcctgcagt aatcattaaa atataatctc cagttttggt atgattttac 54960
gtatctataa tttataataa atgtcttgaa gttgcaggtt tttttccttt gcaatgaatc 55020
aataaattat gtcacaaccc ttaaccccaa gtttttgctt tagcaattca ggataatgcc 55080
aaaaacttac ttagctttta gtctgtgcca ggcattgttc tgaacaaggt acatacaatg 55140
ctgtcttgta gaccctggat ttggaaagcc cccaatccct ttataaagca tatgtataag 55200
ttgtatagat aagtgtattt tcaaaagtaa acatataaaa attcataatt gatatttgag 55260
gatcaaatat tttcctcatt tatgtatcta acccacaaaa atttaattgt ttataagaca 55320
ttttatggag acctttacat ttatatgaag taaaatttaa tatgttcctt aactatccaa 55380
tatatttgag tatctgtgag atatgctatg tgctgaggga tacaattaaa aaatagaaaa 55440
gatttgattc ttgtctttat tttttcaatt atttcaggtg gcaaacacag atggtcacaa 55500
tataatgtga cacctccatt ttcccaatga gaatattaaa taaattgttt tgactaactt 55560
gaatatgtaa actttagaga tgaagatgaa tttctgtata tttatttaag ttatacatac 55620
atatttaaaa tagcattata tgagataatt ttcaaatgta actcttctct cctggctttt 55680
catgaagcaa ttttatctaa actcttcaaa tgcatctttg acatatgagg gtttttttcc 55740
tctctagaac ctctttttga gttgtctgac acagttttct acggttccat ttgaattgtc 55800
tgaaatccaa cctattttga atctatttac gttgtggtca ctggaaattt tctctcagga 55860
gcccctccat tctcattatt aaagatcagt tggttttctt tttcctcctg tagaagcatg 55920
tatgttgatc tctgtagtgt aggcacttat tataatactt aaaaaaagtc atttttgcaa 55980
ggcttgttta caatgaaatc caatatttgc caatgtgagg caatatccct aggagttaaa 56040
tctgtgttaa atctcatcgc agcttttact gcaattttta ctgattcaga tgattgtttc 56100
tgggggactt ctctaagctt ccacagatcc tgttacttag ggtaatgtct tctccaagtg 56160
atactctgtt attcaaagta ggactggaga gagtaaaaag aggttctggt aataagaaag 56220
actttctatg gactttatta atagaaatag aaaacaattt cctattttct gagggatttt 56280
ttttgggaaa agtaaacttt gccttacact tgggcatttg ttgagttgcc agctaacatc 56340
tcttagcggg gagttggtag ataggttcta tttaacccag tgtttttcat gctgttctat 56400
tcaccactgc atactgcttt gccttgccgt gaagtgtact ctttttaccc aattcctcat 56460
tttcatattt ctgttttcat agtatcttgt tagactttta cttcttgagt cagatgaaga 56520
aaacttaatc agtattgatg tcttcagtat cataaatatc tgtaactctt actttgaatg 56580
taagagtaat gttctgatta ctgctaagat tctaccaaat tgactttcag tacatattga 56640
tgtatttctg gaatgaatgt tcctgtgatc ttggcttgca gatgaaaacc ttaatatttg 56700
aatcagactg gtgcaatgtg gaaagacaaa atacacactt gaaatatttg catgataatc 56760
tacggaacaa aaacatttag tgggagtggt ggtagaacgt ttccaggatc aacacttgaa 56820
tgctgtgaga gaactatttt tttttttaat ggatggcagg tggcagagtt gaaaaacagg 56880
tttttataat ggaaatgcta agagtcttag ctataggagt atgaatggta ttgttacaat 56940
agatataata gatgtattgc attatcttat ctcatcagac acttttgtta gtaaaagcgc 57000
ccccccccac tagtttcagt ctgctcttgc agagaatcac tcctctcaac aatttttgct 57060
acttacaaaa tttttgagat tcaagatatc tttaatctgt cgagtttcag cagatttaat 57120
aagcataaat ttattttatt aaaaatattt taagtcagga aagtaagtat aggaaacaga 57180
ccagatgtaa ttcagattac aggatctgga tttgcccctt taatgaatgt cagctatgct 57240
ggcaatattc tgctggtttc cttttttttt ttttttttaa cattactact catatttcac 57300
atttgatttg ttttcaatat ccttactctt aggaaggggc ttaggaaatt gaattacatt 57360
ttatatgttt agttcctaat ctcattctta ttttaatacc tgttcagatt cttaaggctt 57420
tttgtaaata ggggtctgtg gtgtctgtaa gtctgggggc ctaggaggga gtctcacaca 57480
ggagtccggt ttgatcacag tggatagggt actctacagt gtgcttaaag gcaagggggc 57540
tctaagaaag agcagccctg gtcagtgtgt tctgagaaag cagggcagtg tctgagaggt 57600
gagttaaaat aggagtgagt agtcaccatt ttgggatatt agacaggaag tgctttctgt 57660
acatgcaggc tggaaatgca cacaaccctc ctgccccatc cgtgagcagt ccagtgtggg 57720
gagaggaagg aaggtgggat gtggttggtc ataactgtga tcccttttct tctctataat 57780
gctgagtgtt cacaattgag aactgactgg tgagtccttg gtgagttaag gcttaagctg 57840
aggtccctgg gtagttaggt agttctaaga atgtatttgt tcaatagaac ttttgaataa 57900
cttcacttag ctagcttgtt agtgctagga acccaaagat gacaaacagc ccattcctct 57960
gaaaaactgt gactctggtg aggtgcatag ttatttagac ctgccggatg agaataccac 58020
ttttccccgc accaaatcta cctgctggag tgaatttatt tcatgtagac tctgaggtcc 58080
ctctgttgct tttgataacg tgctctcatc tgaggccagc tccgttactt gtatactcat 58140
cctgtctccc ccgccaaggt cattgctcct gcagttttct gctttctctg ttggatcatt 58200
ttccttcttt gctggatcat tcctatcatc aaacattctg tgatatatcc taccttaagc 58260
taatcttgtt tctttttttt tttttttttg agacggagtc tcgctctgtc gcccatgctg 58320
gagtgcagtg acatgatctc ggctcactgc aagctccgcc tcccgggttc acaccattct 58380
cctgcctcag cctccccagt agctgggact acaggcgccc accaccatgc taattttttt 58440
ttatttttag tagagatggg gtttcaccgt gttagccagg atggtctcga tctcctgacc 58500
tcgtgatccg cccgccttgg cctcccaaag tgctgggatt acaggcgtga gccactgcgc 58560
ccggccgata atcttgtttt ttgacaccac attcttactc tccaacttcc tgataccacg 58620
ttcttcctct ccaactattc catttttttc tttctctgta atagcaaaat tccttggaag 58680
gacctgctgc ttctctttct gtttctagtt cactctacgg aggccctttt acttgaaagt 58740
gctctcatca agttcaccaa tgacctttgc ttggccaaat gcaaatgcca cttctcagtc 58800
cttatcttat atagtctttg tttgttagca tttgccacag cttttcactc tttccttctg 58860
gaaatactgt ggccttctgg acactactcc cccttgctta ttggttctct tcctatttca 58920
ctggctgccc cttcagtcat ctttgctggt tccttcttat cataccgaaa tgttggagca 58980
tccccaaggc actttgtttt acttttccac ctatactcac ttatttgggt ttttatagag 59040
tcccatagct tttaggtact ttctctatgc tgatgctttt ttttttggaa ccctgggctc 59100
tctgcttaca tatcatctga atttggatct ttaaaaggca tgtctattta ccatgtccaa 59160
aactactaat ctcccccaca aatctgttgc tccatgttct tcatatctca gtaaaaggat 59220
tctgagtctt ttcagttgct tgagccaaag actttggagt caaggataac tacttgtttc 59280
cacatcacac actgagtcta tcagcaaatc ctgctggctc tacctttaag catatccata 59340
atcctatcat ttttatcacc ttcacttagg ctaccactgt ggtctaagcc agcatcatct 59400
tgcctggatt ataatagtta cctgttttat ctcctagctt ttccctcaac catctgtttt 59460
ccacacagtg atcagagatt cttcgaaacc taagtcagat tattttactt ttctgctcaa 59520
aattcacaat gctgtctagt acaactttct gcaaaaatgg aagtgctcgg ccaggcacag 59580
ggtggctcac tcatgtaatc ccagcacttt gggaggccaa ggtgggtgga tcacctgagg 59640
tcaggagttc gagaacagcc tggccaatgt ggtgaaaccc catctctact aaaaatacaa 59700
aaattagcca ggcttggtgg ccagtgccta taatcccagc tacttgagag gctgaggcag 59760
gagaatcact tgaacctggg aggcggaggt tgcagtgagc cgagatcacg ccactgcact 59820
ccggcctggg tgacgagtga aactctatct caaaacaaac ataaaaaaag gaagtgctct 59880
aatctgtgct aatatggtag ccactggcca tgtgtctgtt aagtacttca gatccggcta 59940
gtgcaactga ggcactgaat tttaaatttt attttaattg atttaaatgt aaatatccac 60000
atgagtggaa gccaagattt atatcatgtt ctgaggttta caaaggccag atgaagtgaa 60060
caccttttct acttctcttg acttcatctc ctgctaccac tgccgccctc attttcttgc 60120
tcttctttga atataccaag catatgcttg cacttgctat tccctcatgc ctggaatgtt 60180
cttcctccag gtgttttttt tttttttttt ttttttttga gacggagttt tgctcttttt 60240
gcccaggctg gagggcaatg gcaagatctc ggctcactgc atcctctacc tcccgggttc 60300
aagtgattct cttgccccag cctcctgagt agctgagatt acaggcgcgt gccaccacgc 60360
cccgctaatt tttgtatttt tagtagagac gcggttttgc cattttggcc aagctgatct 60420
cgaactcctg acctcaggtg atccacctgc cgcagcctcc caaagtgctg ggattacagg 60480
catgagctac cacgcctggc ctccttcttt tcattttagg ggatgagagt tcgaagctaa 60540
cagataataa aacttataaa ttatctacaa accaagaatc tgaattgttg aaattttcac 60600
tttacaagag aagatctagt taggtttata tgataatctt gaactgctac aaaacagatg 60660
ctgtgggctg ggcatggtgg ctcatgcttg taatcccagc actttgagag gctgaggtgg 60720
gaggatcgct ttagcccagg agtttgccac cagcgtgggc agcgtagtga gaccctatcc 60780
ttacaaaaaa ttaaaaaatt agctgggtgt ggtggtgccc acctgtagtc ccagctactc 60840
tggaggctga gatggcagga tttcttgagc cccagatttt gaggctgcag tgagccatga 60900
tcatgccact gtactccagc ctgggcaaca gagtgagacc ctgtctgaaa acaaacaaac 60960
aaacaaaccg cccccctcaa aaaaccaact caatacacca gaaccaaaaa ccaaaaaaaa 61020
cccccagaag ttgggtactg tggctcatgc ttgtaatccc agcactttgg gaggttaagt 61080
tgggcagatt gcttgagccc aggagttcga gaccagcctg ggcaacatag cgagaccctg 61140
tttctatgaa aaatataaaa atttagccag gcatggtggc atgcacctat ggtcccagct 61200
gctaaggagg ttgagtggga ggatggcttg aagcctggga ggtggaagtt gctgtgaggc 61260
aagatcatgc cactgcactc cagcctgggc gacagagcaa gaccctgcct caaaacaaaa 61320
caaaataaaa caaaaacacc acaaaaacca accaaacaaa aaattgttgc tgtgggaata 61380
tttgatgggt ttaatacttg ttaatatcca gtagagataa ttaaggctga tccttgattc 61440
cctttcccta gcattttatt gtggttcatt taggtgttct caatgttgag tgaacttttc 61500
aaaaatactc ttccttctct ataacccaaa gagtatattc ttcaaagcta ccttctacat 61560
gactccctca cccaaaaatt catctttgtt tctagcagcc tacgctactc atttctttga 61620
gcccaccttt gaatttagga cttgtaaaat tataaacatt tataaatgtt tgatctctat 61680
gtccatggaa cagctttaaa aatcttcttt aatctctgag atgaggcagt caagaataag 61740
gcatcttcga ttacagaatc tctttgaagt tttctttcta tttcaggttt tccctctagc 61800
actcctacgt tcggtagctc taaggtggat ttttttaatt aatctttttc ctttttgctt 61860
aggtatcctt ggcagtcttt caaaagttaa gaggcaacat tagtaaaatt atactttcaa 61920
actttatcat tttcgaactc tgtttctcta gtaagttaat gagctagaga cctcctttta 61980
aaataatctc atctatttta cattttaatg ctgtttctgt tgtagatatt acagtcctaa 62040
aaatgtgtta ttttcttaga gtagactgga aagctcaaat taatcaatat gtctccagcc 62100
tagctaccat ccatttctgt tttataggat tatatacttg tttattttat aaaatggaaa 62160
gtttgaaatc ttataatatt ttcatttatg gttttatgtg aatcaaacag tcttggttca 62220
ggtgatttaa aaatatcact acaaaaagga gatatcttgc ttatttttca ttcatgtagt 62280
cctgtgtgaa attattggtg gtctttctgt ctccgtgaac atctgcagcc acacagagaa 62340
attttatttg ccttttgtta tcagattcac atattatgaa tgttgaggct agatatcatt 62400
gatatcattg attagaacca ttaaaatact aaagatacta gtaaagaaaa agatactaat 62460
gacaaaaaaa tactaatctt gcatattaat cttgttttta cttcagtgtt agtaaaaaca 62520
ctagtaaaaa gcagttagtt acttcctttt tattttgtat ttgacattac acacacatgt 62580
tctgcacgtt ctgagaagaa agtgtcagat tgcttcctgt ttgagtttag atgcaatcgc 62640
tagtcttgct gaaagccaat gaaattgaaa ggcttagctt tgtaagtaga atttctgttt 62700
ctttaactct atagttgata atattgtttc tcaagtttcc tttggtaggt attctcaggg 62760
aacgttccag gatcttgtaa tacttcttgt ttgattagaa aatgattcat tacgtgcctt 62820
tcagctcttt aggaaagtgg acggccttca catcagtggg attgaattca gaaaaagaac 62880
atactactaa aagtactttt gtaattattt tattagtttt tctcatatta ttagtttttc 62940
tcatatttca gtgaaatttc ttatattatt agtttttctc atattcagtg aagtgctaaa 63000
aatgatgtcc aaatttatgt ttaaagtttc acagagttat acactgactt taaaattgca 63060
ttgctttatt ttaaggatta gagaaatggt agtgcacggg ttaagaattt gggtgatggc 63120
accaggctgt gtaagtccaa gagccacttt gcttgccatg tgactttgag aaagctgttc 63180
catctctttg tccccagttt tctcatttat aaaatgagga taatagtgct tattccatat 63240
gacagttata agtagtaaat gaatttaaat gaaaatattt aaggtactta aaagtgctca 63300
tataagtagt aggctgctta ttgtattgta ctaatattaa tgaagtatta acagttgtta 63360
atactgtgtt ttaattcttg ctctttcatt tgatagtcat atgactgtcc ccaggttact 63420
ttactttcag tctcattttt catctttaaa aacaagatta tattatgttg tatctaataa 63480
ggccatcatg atgattctag actagatatg caaattacct actatgcaca gagtaagggc 63540
accataattt tatttttttg tttttatttt ttatttattt tattatactt taagttctgg 63600
gatacatgtg cagaatgtgc aggtttgttc aaaacatagg tatacacgtg ccacggtggt 63660
ttgctgcacc catcaaccca tcatctacat taggtatttc tcctaatgct atccctctcc 63720
tagcccccca ctccccaaca gcccccggtg tgtgatgttc ccctccctgt gtccatgtgt 63780
tctcattgtt caactcccac ttatgagtga gaacatgcag tgtttggttt tctgttcgtg 63840
tgttagtttg ctgagaatga tggtttccag cttcatccat gtccctgcaa aggacatgaa 63900
ctcatccttt ttatggctgt atagtattcc atggtgtata tgtgccacat tttctttatc 63960
caatctatca ttgatgggca tttgggttgg ttcctagttt ttgctattgt gaaaagtgct 64020
gcaataaaca tacatgtgca tctgtcttta ttgtagaatg atttataatc ctttgggtgt 64080
ataccgagta atggggttgc tgggtcaaat ggtatttctg gttctagatc cttgaggaat 64140
tgccacactg tcctccacaa tggttgaact aatttacact cccatcaaca gtgtaaaagc 64200
gttcctattt ctcgacatcc tctctggcat ctgttgtttc ctgacttttt aatgatcgcc 64260
attctaactg gcatgagatg gtatctcatt gtggttttga tttgcatttc tccaatgacc 64320
agtgatgatg agcttttttt catatgtttg ttgaccacat aaatgtcttc ttttgagaag 64380
tgtccgttca tattcttcgc ccactttttg atggggttat ttttttcttg tatatttgtt 64440
taagttcttt gtagattctg gatattagcc ctttgttaaa tggatagatt gcaaaaattt 64500
tctcccattc tgtaggttgc ctgttcactc tgatgatagt ttcttttgct gtgcagaagc 64560
tctttagttt aattagatcc catttgtcta ttttggcttt tgttgtcatt gcttttggtg 64620
ttttagtcat gaagtctttg cccattcctg tgtcctgaat ggtattgcct aggttttatt 64680
ctagggtttt tatgatttta ggtcttacgt ttaagtcttt aatccatctt gagttaattt 64740
ttctataagg tgtaaggaag gggtccagtt tctgttaggg gtccagtttc agttttttgc 64800
atatggctaa ctagttttcc cgacaccatt tattaaatag ggaatccttt ccccatttct 64860
tgtttttgtg agctttgtca aagatcagat ggttgtagat gtgtggcatt atttctgagg 64920
cctctattct gttccgttgg tctatatatc tgttttggta ccagtaccat gctgttttgg 64980
ttaccgtagc cttgtagtat tgtttgaagt caggtagcct gatgcctcca gctttgttct 65040
tctgcttagg tttgtcttgg ctatacgggc tcttttttgg ttccagatga aatttaaagt 65100
agttttttct aattgtgtga agaaagtcaa tggtagcttg atggagatag cattgaatct 65160
ataaattact ttggatagta tggccatttt catgatattg attcttccta tccatgagca 65220
tggaatgttt ttgcatttgt ttgtgtcctc tcttatttcc ttgagcagcg gtttgtagtt 65280
ctccttgaag aggtccttta catcccttgt aagttggatt cctaggtatt ttattctcct 65340
tgtagcaatt gtgaatggga gttcactcct gatttggctt tctgtttgtc tgttattggt 65400
gtataggaat gcttatgatt tttgcacatt gattttgtat cctgagactt tgctgaagtt 65460
gcttatcaga ttaagaagtt tttgggtttt ctgaatatac aatcatgtca tctgcaaaca 65520
gaggtaattt gacttcctct cttcctattc gaataccctt tatttctttc tcttggctga 65580
ttgccctggc cagaacttct aatactatgt tactatgttg aataggagtg gtgagagagg 65640
gcatccttgt cttgtgtggt ttttcaaagg gaatgcttcc agcttttttt tttttttttt 65700
tgagacagag tctcaccctg ttgcccaggc tggagtgcaa tggtgcaatt tcggcccact 65760
gcaaccttca cctcctgggt tcaagcgatt ctcctgcctc agcctcccac gtggctggga 65820
tcacaggcat gtgccaccat gcctggctaa ttttttgtat ccttagtaga gatggggatt 65880
caccatgttg gatgggctgt ctagaactcc tgaccgtgtg atccacccgc ctcggcttcc 65940
caaagtgctg ggattacagg tgtgagccgc tgtgtttggc ctcagctttt gcccattcag 66000
tttggtattg gctatggatt tgtcataagt agctcttatt attttgagat aggttccatc 66060
aatacctagt ttattgagag tttttagcat gaaggggtgt tgaattttat cgaaggcctt 66120
ttctgcatct attgagataa tcatgtgttt tttgtcattg gttctgttta tgtgatggat 66180
tacatctatt gatttgcgta tgttgaacca gccttgcatc ccagggatga agccgacttg 66240
atcgtggtgg ataagctttt tgatgtgctg ctggttttgg tttgccagta ttttattgag 66300
gatttttgca tcaatgttca tcagggatat tggtctgaaa ttttcttttt ttgttgtgtc 66360
tctgccaggt tttggtatca ggatgatgct ggcctcataa aatgagttag ggaggagtcc 66420
ttctttttct gttgtttgga atagtttcag aaggaatggt accagctcct ctttgtacct 66480
ccagtagaat tcggctgtga atccatgtgg tcctgggctt tgttttggtt ggtaggctat 66540
taattactgc ctcaatttca gaacttgtta ttgctgtgct cagggatttg acttctttct 66600
ggtttagtct tgggagggtg tatgtgtcca ggaatatatc catttcttct acagtttcta 66660
gtttatttgc ttagagatat ttatagtatt ctctgatgat agtttgtatt tctgggggat 66720
cagtggtgat ctccccttta tcatttttta ttgcgtctat tcgattcttc tctcttttct 66780
tctttattag tttggctggt ggtctatttc attaatcttt aaaaaaaaaa aaagaaaccc 66840
agctcctgga ttcattgatt ttttgaaagg ttatttgtct ctctatctcc ttcacttctg 66900
ctctgatctt agttatttct tgtcttctgc tagcttttga atttgtttgc tcttgcttct 66960
ctagttcttt taattgtgat gttagggtgt caattttaga tctttcccac tttctgctgt 67020
gggcatttag tgctataaat ttccctccaa acactgcttt atctgtgtct tagagattct 67080
ggtacgttgt gtctttgttc tcattggttt caaagaactt attcatttct gccttaattt 67140
tgttatttac ccagagtcat tcaggagcag gttgttcagt ttccacgtag ttgtgtggtt 67200
ttgagtgagt ttcttaatcc tgagttctaa tttgattgca ctgtggtctg agagactgtt 67260
tgttatgatt tctgttcttt tgcatttgct gaggagtgtt ttacttccaa ttatgtggtc 67320
agttttagag taagtgtgat gtggtgctga gaagaatgta tattctgttg atttggggtg 67380
gagagttctg tagatgtcta ttaggtctgc ttgttccaga gctgagttca agtcctgaat 67440
atccttgtta attttctgtc ttgttgatct gtctaatgtt gacagtgggg tgttaaagtc 67500
tcccactatt attgtgtgag agtgtaaatc tctttatagg tctctaagaa cttgttctgt 67560
gaatcggggc gctcctgtat tgggtgtgta tacatctagg ttagttagct cttcttgttg 67620
cgttgatccc tttaccatta tgtaatgccc ttttctctgt gtcttttgat ctttgttggt 67680
ttaaagtctg gttcatcaga gactaggatt gcaacctctg cttttttttt gcttcctatt 67740
tgcttggtaa atattcctcc atccctttat tttgagccta tgtgtgtctt tgcatgtgag 67800
atgggtctcc tgaatacagc atgccgacgg gtcttgactc tttatccaat ttgccagtcc 67860
gtgtctttta gttggggcat ttagcccatt tacatttatg gttagcgttg ttatatgtga 67920
atttgatcct gtcattatga tgctagctgg ttattttgcc cattagttga tgcaatttct 67980
tcatagcatt gatggtcttt acaatttggt atgtttttgc agtggctggt actggttttt 68040
ccattccata tttagtgctt cattcaggaa ctctagtaag gcaggcctgg aatctctcag 68100
catttgcttg tttgtaaagg attttatttc tccttcactt atgaagctta gtttggctgg 68160
atatgaaatt ctgggttgaa aattcttttc tttaagaatg ttgaatattg gccgccactc 68220
tctgctgact tgtatggttt ctgcaaagaa atccactgtt agtctgatgg gcttcccttt 68280
gtgggtaacc tgacctttct ctctggctgc ccttaacgtt tttttcttga tttcaacctt 68340
tgtgaatctg acgattatgt gtcttgggga tgctcttctc caggagtatc tttgtggtgt 68400
tctctgtatt tcctaaattt gaatgttggc atgtcttcta ggttggggaa gttctcctgg 68460
atagtatcct gcagagtgtt ttccaacttg gttccattct ccccatcact ttcaggtaca 68520
ccaatcaaac gtaggtttgg tcttttcaca tagtcccata gttcttggag gccttgttca 68580
ttccttttca ttcttttttc tctaataatg ggttatttca ttaagttgat ctttagtctc 68640
tttagtctct gatatccttt cttccgcttg atccgtttgg ctattgatac ttgtgaatgc 68700
ttcttgaaat tctcatgctg tgtttttcag ctccatcagg tcatttatgt ttttctctaa 68760
actggttatt ctagttagca attcctctca ccttttttca gggttctttg cttccttgca 68820
ttgggttaga acatgctcct ttagctcata ggagtttgtt attacccacc ttctgaagcc 68880
tacttctgtc agttcgtcac actcattctc tgtccagttt tgttcccttg ctggcgagga 68940
gttgtgatcc tttggagaag aggcattctg gtttttggaa ttttcagcct ttttgcgctg 69000
gtttttcctc atcttcgtgg attcatctac ctttggtctt tgatgttgtt caccttcgga 69060
tggggtctct gagtggacgt cctttttgtt gatgttgatg ctattccttt ctgtttgtta 69120
attttccttc taacagtcag gaccctctgc tgcagggctg gtggagtttg ctggaggtcc 69180
actccagacc ctgtttacct gggtatgacc agcagaggct gccgaacagc aaagattgct 69240
gcctgttact tcctctggaa acttcgtctg ccagatgcca gccggagctc tcctgtatga 69300
ggtgtctgtc aaccccttct gggaggtgtc tccaagccag gaggcacgag gatcagggac 69360
ccacttgagg aggcagtcct gtcccttagc agaatttgag cactgttctg ggagatctgc 69420
tgccctcttc agagccggca ggcaggaatg tttaagtctg ctgaagctgt gcccacagct 69480
gccccttccc ccaggtgctc tgtcccaggg agatgggagt tttatctata agcctctgac 69540
tggggctgtt gactttcttt cagagatgcc ctccccagcg aggaggaatc tagagaggca 69600
gtctggctac agcggctttg ccgagctgtg gtgggctctg cccagttcaa acttcctggc 69660
agttttgttt acactgtgag gggaaaaccg cctactcaag cctcagtaat ggtggacatc 69720
cctccccaca ccaagcttga gtgtcccagg tcaacttcag actgctgtgc tggcagtgcg 69780
aatttcaagc cagtagatct tagcttgctg ggctctgtgg gagtgggatc tgctgagcta 69840
gactacttgg ctccctggct tcagccccct ttccaggaga gtgaatggtt ctttctcgct 69900
tgcattctag gcgccactag ggtatgtaga aaaactcctg cagctagctc agtgtctgaa 69960
tggctgccca gttttgtggt tgaaacccag ggccctggtg gtgtaggcac ccgagggatt 70020
gtgaagacca tgggaaaagt gtagtatctg ggctgaagtg cactgttcct ctaggcacag 70080
tccctcacgg cttcccttgg ctaggggagg gagttcctcg atgccttgtg cttcccaggt 70140
gaggcaacgc cccgccctgc ttcggcttgc cccccatggg ctgcccccac tgtctaacca 70200
ggcccgttga gatgagcctg gcacctcagt tggaaacgca gaaatcaccc gccttctgtg 70260
ttgatctggc tgggagctgc agaccagagc tgttcctatt tggccatctt gccagccacc 70320
cctttatttt tattttttat ttatttttat ttttattttt tcagacggag tctcgctttg 70380
tcgcccaggc tggagtgcag tggtgtgatc tcaactcact gcaacctctg cctcctgagt 70440
tcatgccatt ctcctgcctc agcctcccga gtagctggga ctacaggcgc ctgccaccac 70500
gccttgctaa ttttttgtat ttttggtaga gacggggttt caccgtgtta gccatattgg 70560
tcttgatctc ctgatctcac gatccgccca cctcagcctc ccaaagtgct gggattacag 70620
gcatgagcca ccatttttta tttttttgag gcagagtctc actctttgaa ccaggctgga 70680
gtgcagtggc ataatattgg ctcactgcaa cctctgcctc acaggttcaa gtgattctca 70740
tgcctcagct tcctgaatag ctgggattac aggtgtgcac caccatgccc ggccagtttt 70800
tgtattttca gtagagacag ggtttcacta tgttggccag gctggtctca aactcctggc 70860
ctcaagcaat ccacctgcct cggcctccca aagtgctggg attacaggcg tgagccatca 70920
cacacagccc cataatttta ttaatagtag ttgtagttca gtttttataa ttttctctcc 70980
tgaaaaccct gccggatacc cattttcctt tttgtttaat ctattgtaat atttaactgt 71040
atcctgctaa attgatatct agagagtttc taaaaggagg aactcttggg attcagggct 71100
ttagacacga ttgaaataaa tgtctttctt aattgcagac cgtgagagaa acgtggtggt 71160
gatcattaag tttccactac tgagtgttaa aaaaggatgt gagtgctgag ccaacatttt 71220
ctctctcttt tatctcctgt tattccctcc tctctgtcca ttaatatctg ttgtgaggtg 71280
gttatggcag ctggagttat agtgcggaca tgatagtgct gtaataggaa ctgcagtcct 71340
ttcattttat agtgatggca tgggagccac ggaaagctgt aaagcattgg agggttacaa 71400
gagttccaga agtactgtaa gaaacctgaa gttgcatttc cttacattct tatatttgtg 71460
atggttatag ttagaaaaat cactggaata atttggagtc tagggaaagc tttggtgtgg 71520
gctagttttg tatgaatatt tacttttgtt aatattacat accaattttt gtttctaaat 71580
ggaaacctta gcttactgag cttcacattg attttgagga ctgtgtagtg gtgattgaaa 71640
aataggttac agccattttg gaagagagaa tacactggac ttttacatga agtatggcat 71700
acgatacata tctgacacat cttgctaaaa agcaagtcct taatgacatg acctcccatc 71760
ttgagcatag acacatgttt taaattcata aatgatggcc tattgagaga tatctattgt 71820
gttcattaaa atatacatat gaaacgagga tcaaattaaa catttcaagt agctaatttt 71880
atcagctctt aataattaat tagtttttag catttagaat gaaggtaatt aaaatagatt 71940
tactacatgc tataagattt ctgcctctgg aagatgaaag gggacattta tttttcatat 72000
gcttaaacat tggcgttttt gcttttgttc tatggggatt ttgtatgtaa caaaaaaact 72060
tactgagtta ttttattatg aaattttaat tataaaatat ctttaatgct ttttagtcgg 72120
cctaaattta attgtagttt gttggaatgt tacaatttta ttttaaattt ctaaagttaa 72180
tataaaagtc ttttctgcta ctgtggtgta ttgttatatc attttattta tatttttata 72240
atttgatttt taaagtgggt catatcttta catagttcaa aatacccaaa atataaaggc 72300
aaaagttttt ttcctgtctg gattccttat ttgcttggtt ctcctataat ctccaggtaa 72360
aaacattagt ttcttgcata tccttccaga gtttatacaa atgcaagtag atatacgtac 72420
atattataag ttttttcttt tatcacattt taaaaagtat aaatccagag atattaagta 72480
atagataata agtgttagat gtcagataat aacttgtaaa gaataaagtt gcattattgt 72540
tatacttgga aataaaataa tttaattaca tgtctctctc cagttatttc aggttgagtg 72600
tacgtttgct tcgtcttcaa agtagaaagt tcctggagaa gacctactct ttctggaact 72660
gtttttgttt gacccatgct atgcaaactt actcattcat gcaaacactg ggtgctttat 72720
gcaaatatta ccacctgtat tttttatttg tgtttctcaa aggagttttg gcaaaatttg 72780
cataatgact tggctcattt agctttgtgt attgttaaga gttcaaattc tgtggatatg 72840
aaagtttaca gtgtatacca aataaaaagg tcgtgtggta ggcagtgttc tcaggataag 72900
cctgtgttag gtattcaaat ctaatttgca tattggatct agacctatga ctattaactc 72960
catttacagt tgataaaaat ttgcataaaa ttggtcatct tgatagctta aggtatactg 73020
agcaaaatgt taactccttg ggtagcatgt gtgactgtat gattgtcaag atgaaagtta 73080
atctaagcac atttggttta ctgataatca tttctagaaa tgagctgtag tatcatgtca 73140
gaatgtcaga aacatgtctg acaatttacc ttttcatact agaaatggtg gatgacatgt 73200
ttcaaatcct ggcagctgtg ctgtatcaaa tcctccctat gtgaatatca tgcttggtac 73260
ttagatagtt taggagcagt actgtggctt gtgatttgtt gtataattct gaaagaaaat 73320
atattaattt tgtgcacttt atattggctc ttgctttatt gtagccttta tccattaatc 73380
tgctactgat ggataatttc tcaatcattt ggtccttttt gtaatggcta tgtggagttg 73440
gcaattcttt ttattaaata ttgcatgact gtatatgtgt taataattta ttggggtagt 73500
gagctgatat aaatctcttt ttagatgaag tttttggcta tttacaaagg ttgattactt 73560
tttagaacag tgattgcctc agatttgcaa aggttgagga aaagcacacc tcttatttta 73620
cctttttcca tttgtggtta aacctattgt aaatatattg gaagggtttt aggcccttga 73680
agacgcctct gtatttgaac agtttgattc tgtcaatttc atttaaatca ttattttttg 73740
taagacctca ggataaatac cacttttgag gtagtgactg aacaaacatg agttcttgaa 73800
tagtttctat taaaatgttt tttttaatcc tatgagtggt ctctgtgcct tttaaaataa 73860
gattttaaat aagatgataa ttaatactca agatatgata cttttattta tagtgatttg 73920
gttgccttga tcatatttaa tgatgattta aaaaacctgg atggagagag agatatatat 73980
gtatacatat atatatatat atacacacac acacatgaat gataaattat tctgtaatat 74040
tatttataca ttcattgttt ctagctagca agcaccctta gtgtatttaa tgtattagat 74100
ttatataatt cactctatgt acaatattag attaggatga gaattatatg aaatgaggca 74160
tgccactatc aagtctggca tgtttttgtt accaagttct gagtgctgaa tgagaaacag 74220
tcatgtattt actcagattg atcccggatc ccctgaggga attggcagtt tcctctggat 74280
ggcattgcta aatcttcagt tttctttctt cctcgctctc tctgtggccc atacttaggt 74340
cagattactg agaaaatcag tgttattgac gaatgccttg tacatgatta taaaagtctc 74400
aatacctctg agaaagatca gaaacacata attaaatgca aatagggtaa cttatttaat 74460
tttgagttaa ttatagcttc atccaatgtc attgattact ggggcagcac aacttggtgt 74520
ccatctagta cagatgatag tgattcttat tttttctttt agatatatta atgcttgact 74580
ttgaggataa cacattttta attcagaact agtgtttaag ttttttgttg ttaagtttta 74640
acaaaagttt ttcttgtggg catgggaatg gtaagtagtg attaaagttt tgaatttaat 74700
ggatataagg aagaaaaata aactgagaat tgacttacat tttttcttgg agaggagggg 74760
attcagcact ctcagagcct ctcctctgtt agcgtgctgg tagttgatga ggatgaagtt 74820
gatgagatga gcaagaatct ggaaggacca agcataggta tactaattac acagaacttt 74880
aaaaatccgt gtatgattat ttcattgcat tgtagaattg cagatgagaa aagaatttgc 74940
aatatttaac tcaaatttgt tttatagcta aaataaccga ggcccgcaga gattgggaga 75000
tgtgtgtgaa gccattgaat gagttactgg cagcctagaa tccagatcat atttattcaa 75060
cctaatgcct tcttctgcat tattctcttt ccatgtttat ttgttataca tacagtagtt 75120
gttattggct tgctagatgg ttatgtatga ttgagaaagg aggtaggaag acctaatgaa 75180
aagtttattt cacgattctg ctgaagactt ggtctgctga cagttaataa tagtagctaa 75240
tataacattg agaacttgct gtgtaaggga cactgtgcaa agagcttcat gggtacttac 75300
atgtaatctc cacagagttc tctgaggtag atagcagtat tataatacta ttttgtagat 75360
gcaaaaacca agtcttaaat aggtgaagta atttgctaaa gatacttatc tactcagtat 75420
gggggcacat atttgaactc ctaccatatc ccattctgct tccctttatt tatcagtcag 75480
catataaggg aaggtctttg aacagatcca aagcccttgt cagctctcat gtccagtgac 75540
tactggaatt taagtttttt ccctctttttctaagaaaat ttttcttttc attataatat 75600
gaaaatcagc taattgaatc tcaccccaga tttcaaggga ttggactcct tcatgtcaag 75660
tttattctgg atagtctctc agcccttagg gaggaatggg gttagcttgc tattattgct 75720
tgctattatt tggtcctcag gattttaggc ttttacgtcg tggttagtggt ttatcaacc 75780
atcctgaaaa tggcataatt atagtctttt gaggattgat ggtagatgaa caggttacct 75840
gatttacctt tccttgggaa gttggaacac ttgcctacct cttgcctttc catattttct 75900
tctttctcca tgattcctca gaggttactt acttactgat tcagcagcct cacttggttg 75960
ttctttgatt ccctgggata tcagttttct gggctgggtt tggagcagtg aggcattctt 76020
tattcttctt acccactgtg gcctctaatt ccttttaacc atattcattg tatgcttttc 76080
tcatctgaag aagatggtca catccagttt ctcagtgtga ggatgaaaaa ctcactggta 76140
atttttgttt tgggtctcct tattgattag gactctagtc ctgtcatact gattaaatct 76200
cagaagggta tagatgtagg gagtggaatc tgcttgttcc cactgagttc ggaaacaagg 76260
aggatactgt gagcaaaggt ttattcagtg atttttcttc ctcttctact ttttcttttt 76320
ctgtgtatta tttggttctg gcaagtacca ataaaagaat caccgtgcag acctctagga 76380
ttttagagca aatttgtgcc ctctctgctg ataatgattc ttcttttgag aaacagttcc 76440
tggcttttga ctttgctaga gactgaacac ctactcatga gatctcaggt gaccatgtgg 76500
ctgagtttcc catcatgaat tggctgttgt ctgacctgtt tagccatagg ttgggaacaa 76560
gcagcagcta tctgttatta agtgaaaatg gtatataaga gaccaaggtt gggcaggtcc 76620
agataatata agtaagttaa acgagctggt ggctcagact cctttgatac caacttctgt 76680
tccattgctt ccactccttc actccatgtg tatggtctcc tgggatgttc cttatggacc 76740
atttgagtga agaagaaaat ttgagcctag tttataagtg ggtctggatg atatgcaagt 76800
gttactcaga agcagacacg gaaacactaa agccccacaa aagactcacc atgaaggaca 76860
atggtgaagg aagatcttcc cagtggacag aacttctgtg cattatattt tgttgcctac 76920
tatgtctgca gtaggagatg gccagggaca aagatctaca ttgattaatg ggcagttgct 76980
aaattttttt tttttttttt tttttttttg agacggagtc tagcttagtc acccaggctg 77040
gagtgcagtg gtgtgatacc ggctcactgc aacctccacc tcccgggttc aagcgattct 77100
ccagcctcag cctcccagat agctgggatt acaggcgctt gccaccacgc ccggctaatt 77160
tttgtatagt tgctaatgtt ttatcttggt ggtcagagac ctggaacaaa caggatgtga 77220
agactggtga caaggaagtc tagggaagag gtgtgtggat agacctctct gaatgtccac 77280
aaactgtgaa aattgagtgc catgtgaatg cctgttaaag ggcatctact gcaaaggata 77340
cttgctagtg agctggacag aacaacgtgc tgtgtgcatg tcagtcagcc ttttattcac 77400
ccacattagg gcttgctcag tggacccatg aaaaggtggc tatattggca gggttgggag 77460
ctcttcatgg gttcaacaat aaggtctttc cctttacaag actgattggc tactgttatt 77520
gctgagtgcc taatcagcca actgcagagt ccaacattga gtccctgata tggcaccatt 77580
cactgaggaa ccagccagcc atctgctagt ggttgattat attggacctc ttccatcatg 77640
gagggggcaa agatatattc tcacagggct agtagcatat tctgcatatg gatttgcttt 77700
ctctacctat aatgcttctg ccagcaccac catctgtaga ctcacataaa actttattca 77760
ctgttagggc attctgcatt gtattgcttc tgatcaagaa ttccttctct agcaaagaag 77820
gtgcggctat gggctcgtgc tcttggaatt aactggtctt gccccatacc ccgtgatctg 77880
aaagtggcta gcctaattga aaggtagaat ggcttactga atactcctaa tgctaattga 77940
aaggcagtac catgaaagga tggggttctg ttttacaaga tgtagtatac aatttgaatc 78000
tgtgaccaat acatgtgctg tctctttgac tctgggattc aaggggtaag tgagagtagc 78060
tttgctcatt gttacaccta gtaactcact tgcagatctt ttatttcctt tcttggaaat 78120
actgagcttt gctgagctct ggtttggaga ccttggactt caaggggata gtgtttccac 78180
tagaggatac aacagtggtt ctatgagatt ggaagatggg gctgcttctt ggccattttg 78240
cagtccttac actactgagc aaatactcag aaagaggagc taatttagtc actggagtaa 78300
ttgatactga taaccaaggg gaaattgggg atttgataca ctgcagtcaa agagacaata 78360
tctgaaattc aggggattct ctgggcacct cttagtactt ccatccccaa cagtgaaggt 78420
taatagaaaa taataccagc caaaaaaggg caggatgatt aatggctcaa atatttcaag 78480
aaggaaggtt tgggtcactc caccaggtaa agaactcaga gcagctgagg ttgtttcatc 78540
tctggagctt tgatctgggt cttcttttta atatcctcga gttctatgct taatttttgg 78600
gtacactaat aatggttata ataacttttt aatgatttat ctattctaac atctgtgcca 78660
attctgggtc agttcttatt ctccaatttt tcttctcatt atgggattcc tttttcttat 78720
tttttacatg cctggtactt tttgataaga tgctaaacat tgtaaatttt acctttttgg 78780
ttgctggata tttttgtgtt cttataaata tttttgagct ttgttctgga tgcagttaaa 78840
ttacttggaa acaggttggt tctgttcagc atgactggag ccatgctcag tcaagggcta 78900
attattccct gctactgagg caagatcctt ctgtgtgctt tactcagtgc cccatcagtc 78960
gtgaaatttt catgactggc tggtaagaac aggcattatc cccagtactg agtcagctct 79020
gggcgccgtt actccattct tttgaggtca ttctttcccc agctttggtt ggtttcctca 79080
catacatgca ctggtcaatg ctcagctgag tactgtaatt actcctctca agtactctgt 79140
cttacaaact ctagcaagcc ttgatttcct caaattctta actctgtctt agtgcagagt 79200
cttctggact gactgccttg cacaactgca gccaagtctg gaaactgaca aagcagtaag 79260
ctggggcaat tgtagggccc acttcatttg ttttccgtct ctcaggggtc actgttctgc 79320
attgtgttat gttcaatgtc ttgaaaaatt attgttttat atgttcggcc tgttttttgg 79380
tcgtttcatg tggaagcttt taatgttaat gttatttctg agaccatata tttatataac 79440
attgaattgg ataagattaa acagaagaaa cattatttag aaagttaaaa aaatttttct 79500
gcgtgagagc ctgttgttag ttatttagaa tcacttctta atcacagcaa gatactttgt 79560
tttattgaag atttgtttcc ctctagattt tctccttatt aaatagtaga acatagagaa 79620
gagaacagtt aaggtcggcc ggattgcttc tgcaggtttg tgtccataga gaagctgaat 79680
gtgtagaatt tcaagaaggt aattgagaag tccgggagta gagtttatct caacaccaaa 79740
agaatcattt ttaaataaat acagggtgag aaaagtgaag aatcaaaaga gtagcttttg 79800
aagcacagta ttcctaaggt cttgttttaa ggatatgttt ctgtgtttgt tttttattga 79860
tttgagatca tcttaatgcg tgttagaaaa tttatcaggc tgaacgagtt gatcctaaaa 79920
tgctgcagaa aagttggatg tgaaatggga aagaaaacta actttcatga gatgcttcgt 79980
aaaatctatt tcaattcagg gtgaaaattg caaaactgtc tgccaatgcg tttttgatgg 80040
acattctggc tatttgtgtg agtcttctgc attgaggttt tatttattga gcttcctttt 80100
gcttcactct agaccaggtg gtttacatat gttatctcat cttgtccttc tgacaaatcc 80160
tgtgaggtat gtggtgttaa tctcatttta aagatgaaga ttttcaggct tatagggttt 80220
aggtagttga cttaaggtca ctggtagtaa gcagggatta tggctgggtc tttgactgct 80280
aggcaagtat taacacctca tcacacacat gggctgcttt ttctgaaggt agtaggtgga 80340
gatttctttc cttgagtaaa agggcaaaaa agaaagaacg taaagtagtt tttagaagta 80400
gtttcttctg gtcaccaaat taacaggaaa agggaaactt gtctaaccta ggtgttgtgt 80460
tttccccatt attctgacta cagtaatttc tgttgaaata accacagtca ttttgttgaa 80520
aagccatacg tgtttctaaa taaaatagtt acataatttt aagtgcatat tttaattaat 80580
tacacattct tttaaattat tcatagacta tcatagattt ttatatcagt tggcatctgg 80640
agtcacaatg taaatattag aaaaaagatt cagaggacct ttttcccaaa taaaatcatg 80700
tgaccctaaa gaacaattta aataacacta taaaaacaga gttatcagga gagggagtgg 80760
tggctaccga tatttaccat tttttcttgg tggggattta taggaataga atacagtcag 80820
ttcctctatt tatttatttt tgtttgtttg tttaaacccg ttgctgttgt gtcattaaga 80880
atgtaaaata atgagatagc ttttttcttt tttctttttt ttttcttttg aacaagtctc 80940
actcttttgc ctaggctgga gtgcagtggt gcgatctcag ctcactgcaa cctccgcctc 81000
ccaggttcaa gcaattctcc tgcctcagcc tcctgagtag ctgggactag aggcatgtgc 81060
caccacacct gactaatttt tgtattttta gtagagatgg ggtttcacca tgttggccag 81120
gttggtcttg aactcctgac ctcaagtgat ccacctgcct tggcctccca gagtgctggg 81180
attacagaca tgagccactg cacccagccc aaggtaactc ttaataaata cagcaatagc 81240
tcagggtact tacatctgag tgtgttctgc tgttcctgaa ataaattaac atatgcatag 81300
taaactctaa agttatcacc atttgggtac atagaagttt actgaggaaa gaagtttagt 81360
gtctgtcata taaacagcat tattatcctt ggtaatgtct gaagtataca gccaaagttg 81420
agaaagtttt tgaactaata aaaatatatt ttcattgatt atgagagtga aattggtaca 81480
tgtgtaattt atatatatat aaagtacaca agttatctat atatagtttg tatacttaag 81540
gaaattgaaa atttaagaaa ttgaaaatct actacttttt agtacttttt aggtttcttt 81600
ccagttctaa gtgagtgtga ctttgtataa ctgcattgta gtatgttctc ctctatgcaa 81660
aaataaacaa gtaaataaaa cccagtgtta tatccttgac gaggacacta ttttgttgct 81720
gacatatggt tagtcatgag ttgtattcag taccatacct tcctcattta gcagcaccat 81780
ctgttaaaac tatggctgat accagtagag aggaagccaa ttatttttga gtttgtgagc 81840
atgggataaa tgcgaatatg tctgaagttg cccgcagtca aatcctttac aactggtgta 81900
gtcaaacaat tggaggtagt gctaccttgg ggaaccaggt caggaaagaa catgataatg 81960
cttgttcggc atctttctct actcttagca agggtgatgt gttataaaat taaataacac 82020
ataattaagt tattaaaaag atttgcatga tatgctttgt ttttaaggct ctcaaactat 82080
tggtgtttgt ggtttgtgac tgcagctggc ataataagag ttggtaaagg tcttttcaca 82140
atgaggcaaa acctttacag gaatgatttt cttcataggc tttttatgga tttgggagag 82200
tattgtacta ataaagttaa gcatgaagtt attagacatt tatcattgat ttcatgtctg 82260
tgtctttatc tgtgttacga atgctttgga ctgcacatat gggaatatag cagaataact 82320
ggccttggag ggacattggg tttttcaaaa cagacctctt atctgtgtgt gtgctggcag 82380
caagtatgta tttttcgatt tccacctgga ctcttggagc acctttttat tttcttcttc 82440
tttcttcctt cttccttcct tcttcttcct ccttccttcc tcctccttct cctccttctc 82500
cttcttctcc ttctcctcct cctcctcccc cctcctctct ccctcctccc tctcttttcc 82560
ctccttctcc tcccttcttc tccttctcct tcttcttctt cttcttcttc ttcttctttt 82620
ttttttttga gatggaatct cactctgtca cccaggctag aatgcagtgg cacagtctcg 82680
actcactgca gcctccgtct cccaggttca agtgattctc ctgcctcagc ctcccgagta 82740
gctaggatta caggtgtgca ccaccacgcc cagctaattt ttgtattttt agtagagatg 82800
gggtttcacc atgttgttca ggctggtctc aaattcctga cttcaagtga tctgcccacc 82860
ttggcctccc aaagtttatt attattattc atcttaatgt tctccttaat attgggacca 82920
tattctgcta aatgaagcat ggtatatatg atttttcctt tttctagagg tttatctttg 82980
gaaaatttta ttttctttta acttttattt ttacttcagg ggtacaagtg caggtttgtt 83040
acataggtaa acttgtgtca tgagggttcg ttgtacagat tacttcatca caggtattaa 83100
gcccagtatc cgttagttat ttttcctgat cgtctccctc ctcctgccct ccatcctcta 83160
ataagcccca gtgtgcgttg ttcccctcta tgtgtccatg tgttctcatc atttagctcc 83220
cacttataag agaacatgca gtatttggtt ttctgttcct gtgtcaagtt tgctaaggat 83280
aatggcctcc atctccatcc atgtccctgc aaaggacatg atctcattct tttttagggc 83340
tgtgtagtat tccatggtgt gtatatacca cattttcttt attcagtctc ccattgatgg 83400
acatttaggt tgattccgtg tctttgctat tgtgaatagt gctacaaaga atatacactt 83460
gcatgtgtct ttataataaa acagtttata tttccatgga tatctgccca gtaatgggat 83520
tgctgggttg aatggtattt ctgtctttag gtctttgagg aatcgccata cagtattcca 83580
tagtggctga actaatttac attcccacca acattgtata agcttttcct ttttctcccc 83640
aaccttgcca gcatctgtta ttttttgact ttaataatag ctgttctgac tggtgtggaa 83700
aacgtttttt tttctttttt tttgaagtag tgacaacttt gaaattatat ttggctctgt 83760
ttttgcccag tcttccctct cctcttccct catgcacaaa cttaccatag gaccaataac 83820
ccatatcagg ccagactttc ccttccctca ctttccctcc ttttctctta ttttcctttc 83880
tgtaagtaaa tcttatccaa aacaggaaac aaacattaat aaaatataaa aggcagggag 83940
acaatggcaa caaaattcta ctttaggcta gaatctaatt cttgagtaat tctttcagaa 84000
aagatctggg tggtaaattc tctgtgctct tgcacaactg aacatgtttc atacttgttg 84060
gaatgagtct agtttttgaa actttaaaat attttttaga gacaggatct tgaacttggg 84120
ctcatgtgat cctcctgtct tagcctcctg aatagcttgg actacaggtg cacaccacca 84180
cgcctgacta gaatgggtct aaatgtttag attcaaagtc attttccctc acagctttga 84240
ggcattcctt cctttagaat ttattctaat agttaagaaa tctggtggcc atctgttttc 84300
tttctctgca ggcatctttt gtttttcatt tttcaaaaac ttttaggcct ttatttttag 84360
ccttagtcat ctaaatatca gaaggacatt cctggtgtga atgtctattc agctacttca 84420
tgcttagggc tctagggatt ctgtcaatct gaggtttcgt gtcttagccc tggaaaggtt 84480
ttaagtttgt ccttaatttc cataccttca ttttctttat gttctccatt tctggcaatc 84540
ctgttatatg tgtcagcttt tgtggactgg tcctttgtgc agtgatccac ctgcctcagc 84600
ctcacaaagt gctgggatta cagatgattt taaagtgatg gtgttatttt tcaggaggct 84660
aactctatta ttggtatatg atagatgggg aagattttac ctgtgaccca gaatgaacat 84720
gtttgttatg tatttcagtt taaaattaat aatgtgtgtg accaagacac tggtgtttac 84780
tattactgtt atgctcttgg ataaagctaa gaatattaaa acttgaaaaa attaatacat 84840
aatatgttac atatttgtgg gtacgtgtaa tattttgtta tgtgcataca atgtgtaatg 84900
attgagtcaa tatttggggt gttcattgcc atgaatattt atcatttctg tgtactggga 84960
acatgtcagg tcctctcttc tagttgtata tttgaaatat acaatatatt gttgctgtct 85020
gtagtcaccc tactcggcta tcaaacatta gaacctattc tttctatcta actgtatgtt 85080
tgtacctatt aaccagtctc tcgttatccc tcctagtcct tggcacatcc ccttcccact 85140
tgcgatatct attattctat tctctacctc catgaggtca acatttttag cccccacatg 85200
agtgacaaca tgcaaaattt gtctttcttt gcctggttta tttcacttaa tgtaatgatt 85260
tccagttcca ttcatgttgc tgcaaatgat atgattttgt tctttcttta tggccaaata 85320
gcattccatt gtatatatgt accacatttt ctttatccat ttttctgtga cagacactta 85380
ggttgatttc atagctttgc tattgtgagt agtgctgcag tgaacatgga agtgcaggta 85440
tccttttgat aaactgattt ttttttcctt tggataaata cccgttagtg ggattgctgg 85500
atcatatggt agttctattt gtagtttttt gagaaatctc aatactgttt tccataatgg 85560
ttgtactaat ttacattacc accaacagtg aacaagagtt cccttttctc tgcatcctta 85620
taagcacctg ttattttttg tctttttaat agtagccatt ctaactgggg tgagatgata 85680
tctcattgtg gttttgcttt acatttccct gatgattagt gatgttgagc atttttccat 85740
atacctgttg tccatttgta tgtttcgttt tgagaaatgt ctattcacat actttgcctg 85800
tttcttaatt agattttttt tttgctgttg agttccttat atattccgct tattaattct 85860
ttgtcagagg aatagcttgc taatattttc ttcctttttg taggttgtct cctcactttg 85920
ttcctggttt tcttgctgtg cagaagcttt ttagcttgat gtaaaaccat ttgtctactt 85980
ttggttttgt tgtctgtgct tttgaggtct taatgataaa atctttgcct agaccaatgt 86040
cctggagtgt tttccctatg taaaatcact tttttgaatg gataatttgt tgaatggcac 86100
aaaattcaaa gggaaccaaa acccatacag taagtaataa gactccatcc atctgccgtc 86160
tgcttcagct aagtatttgt gaagaaactc attgttgcaa tttctacaca cacacacaca 86220
cacacacaca cacacacaca cacacacaca cacacacaca aagggtatgg atttataaga 86280
ataaatgtac acacacacac atatatatat acatacataa tgttttttgc tttctccttt 86340
tacataaaag ttagcatata atacacatga ttttcattta actatttagg ttgttgcaga 86400
agtaattgca gctaatatat gatttggtta ccttgtttta tcagttcatc acaaaatttc 86460
tttctttttt tttttttttt tttttttttt tttttttttt tttttttgag acggagtctc 86520
gctctgtcac ccaggctaga gtgcagtggc gtgatctcag ctcactgcaa cctccgcctt 86580
ccaggttcaa gtgattctcg tgtcttggcc tcccaagtag ctgggattac aagcatgtgc 86640
caccataccc ggctgatttt tgtattttta gtagagacag ggtttcacca tgttggccag 86700
gctcgtatca aattcctagt ctcaagtgac ctgccctcct cggccttccg aagtgctgag 86760
attacatgtg tgagccacca tgcctggctg cgacaaagtt tcttatttta ttttttgaag 86820
tagtagtata ttccattttg aggatatggg atatttatat acagtaaata tttatatatt 86880
tatatataat atatattatt tagccattcc ttttattcta gacatttttg ttgtttcttt 86940
tcttggcttt tgctaataac aaaactgcta cagtgatacc catgtctttg tatttatgta 87000
tcattttgca tatgcataaa tatattttta gactatctgg aagtgtaatt actgggttaa 87060
aagttgtgta catttctaat tttgatagat attacctaat tgccttgtgt agagattata 87120
ttaatttatg ctcccatcaa caacatatat gcttgctggt ttccccagaa ccttgccaac 87180
atagttctat gtatcatgag ttttttttga tctttgccca tctgatgaat gaaaaatggg 87240
atctcagtgt cttttagttt gcatttctta caagggtgat tttttgtttc attttctgtg 87300
acctgtctgt atccctttat catttttctg ttgggtgacg cttactgatt tgtaggtact 87360
ctctatttgg gaaatcaacc cttcatgata tgatttccag atttttcccc ctttgctata 87420
tttttatgtt gtttatggtt gctcttgcca tgaagaaaat aattactttt tacatagttc 87480
actttatcag tgttttcttt tactccaagg tgatgatggt aattctctga ttgtttctgc 87540
tgttacttta atggtttcat tttcttacat ttaaatcttt gagttacatg aaatttattt 87600
tgatttatgc atcattgatg gttccagctc tccttcccct ggatgactag ccatttctcc 87660
tgataccatt tattaattta tcttttctcc tttaattcga atgcatctac caaaagctaa 87720
cttactaaat gtatttgtat tccttctctc atatttaaga gttatgtttg ttcagtatga 87780
aaaagttcaa acgtacccac aagtagaggg aatagctcag caagaccccc ctacttatca 87840
cagattcagt aattaagatt tatctttttt tccttttact tatgtttttg ctgaagaatt 87900
ttaaagcaat cccagataac atgtcatttg cctcttagta gttctgcgtg tagctttaaa 87960
aatatggact ttcaaaagat aatcaaatat ttgacttttt ttgtttatta tactttaagt 88020
tttagggtac atgtgcacat tgtgcaggtt agttacatat gtatacatgt gccatgctga 88080
tgcgctgcac ccactaactc gtcatctagc attaggtata tctcccagtg ctatccctcc 88140
cccctccccc caccccacca cagtccccag agtgtgatat tccccttcct gtgtccatgt 88200
gatctcattg ttcaattccc acctatgagt gagaatatgc ggtgtttggt tttttgttct 88260
tgcgatagtt tactgagaat gataatttcc aatttcatcc atgtccctac aaaggacatg 88320
aactcatcat tttttatggc tgcatagtat tccatggtgt atatgtgcca cattttctta 88380
atccagtcta tcattgttgg acatttgggt tggttccaag tctttgctat tgtgaataat 88440
gccgcaataa acatacgtgt gcatgtgtct ttatagcagc atgatttata gtcctttggg 88500
tatataccca gtaatgggat ggctgggtca aatggtattt ctagttctag atccctgagg 88560
aatcgccaca ctgacttcca caatggttga actagtttac agtcccacca acagtgtaaa 88620
agtgttccta tttctccaca tcctctccag cacctgttgt ttcctgactt tttaatgatc 88680
gccattctaa ctggtgtgag atggtatctc attgtggttt tgatttgcat ttctctgatg 88740
gccagtgatg atgagcattt tttcatgtgt tttttggctg cataaatgtc ttcttttgag 88800
aagtgtctgt tcatgtcctt cgcccacttt ttgatggggt tgtttgtttt tttcttgtaa 88860
atttgtttga gttcattgta gattctggat attagccctt tgtcagatga gtaggttgct 88920
aaaattttct cccattttgt aggttgcctg ttcactctga tggtagtttc ttttgctgtg 88980
cagaagctct ttagtttaat gagatcccat ttgtcaattt tggcttttgt tgccattgct 89040
tttggtgttt tggacatgaa gtccttgccc atgcctatgt cctgaatggt aatgcctagg 89100
ttttcttcta gggtttttat ggttttaggt ctaacgttta agtctttaat ccatcttgaa 89160
ttgatttttg tataaggtgt aaagaaggga tccagtttca gctttctaca tatggctagc 89220
cagttttccc agcaccattt attaaatagg gaatcctttc cccattgctt gtttttctca 89280
ggtttgtcaa agatcagata gttgtagata tgcggcgtta tttctgaggg ctctgttctg 89340
ttccattgat ctatatctct gttttggtac tagtaccatg ctgttttggt tactgtagcc 89400
ttgtagtata gtttgaagtc aggtagtgtg atgcctccag ctttgttcat ttggcctagg 89460
attgccttgg cgatgcgggc tcttttttgg ttccatatga actttaaagt agttttttcc 89520
aattctgtga agaaagtcac tggtagcttg atggggatgg cattgaatct gtaaattacc 89580
ttgggcagta tggccatttt cacgatattg attcttccta cccatgagca tggaatgttc 89640
ttccatttgt ttgtatcctc ttttatttcc ttgagcagtg gtttgtagtt ctccttgaag 89700
aggtccttca catcccttgt aagttggatt cctaggtatt ttattctctt tgaagcaatt 89760
gtgaatggga gttcactcat gatttggctc tctgtttgtc tgttattggt gtataagaat 89820
gcttgtgatt tttgtacatt gattttgtat cctgagactt tgctgaagtt gcttatcagc 89880
ttaaggagat tttgggctga gacaatgggg ttttctagat atatttgact ttttatggcc 89940
aggagcagtg gctcatgcct gtaatcccag cactttggga ggctgaggtg ggcagatcat 90000
ttagcccagg agttcgagac cagcctggac agcatggcga aaccccgact ctaccacaca 90060
cacacacaca cacacacaca cacacacaca cacacactac aaaaattagc tggacatgat 90120
ggtgtgcacc tgtagtttta gttactttag aggcttaggc tggagaatca cctgagtgca 90180
cgaagttgag gctgcagtga gctgtgattg caccactgca ctccaggctg ggtgagtgaa 90240
acctttctgt ctcagaagaa aaaaaaaaaa gcctttttgt tctaaattaa agagaaaaac 90300
cacggggggc ttaatagttg atgtctgtgc ttttactggt actcagttgc tgaaggtggt 90360
atcatattag tcctttaatt gcttagagtt gtgtcctcat gaaactttct ccctgaactt 90420
gcttttaagg gagatctgcc tctgtatgcc atggtattgg aatgttttat tagtgtagaa 90480
gtgtctagat gattttccta gggggccact taatgagcag tctgtgtgta ggccctatga 90540
ataataaaat tgtgatcagt ggttaagtat agccaaatgt atggatagga cctagggaat 90600
aataaagaag tgcttcaaat gtagcttaaa actatttttt tttaaatttt actttaagtt 90660
ctagggtaca tgtgcacaac gtgcaggttt gttacataga tatacatgtg ccatgttggt 90720
gtgctgcacc tgttaactgg tcatttacat tagatatttc tcctaatgtt atccctcccc 90780
ctgtcctcca ccccatgaca ggccctggtg tgtgatgttc cccaccctat gtctgagtgt 90840
tcttattgtt caattcccac ctgtgagtga gaacatgcgg tgtttggttt tctgtctttg 90900
tggtagtttg cttagaatga tggtttccag attcatctat gtccctgcaa aggacatgag 90960
ctcatcattt tttatggctg catagtattc cgtggtgtat atgtgccaca ttttcttaat 91020
ctggtctatc attgaaggac atttgggttg gttccaagtc tttgctattg tgaatagtgc 91080
cgcaataaac atacatgtac atgtgtcttt atagcagtat gatttataat cctctgagta 91140
tatactcagt aatgggatcg ctgggtcaaa caatatctcc agttctagat ccttgaggaa 91200
tcgccacact gtcttccaca atggttgaac tagtttatac tcccaccaac ggtgtaaaag 91260
tgttctattt ctccacatcc tctctggcat ctgttgtttc ctgacttttt aatgatcgcc 91320
attctaactg ctgtgagatg gtatctcctt gtggttttga tttgcatttc tctgatggcc 91380
agtgatgatg agcatttttt catgtgtctg ttggctgcat aaacgtcttc ttttgagaag 91440
tgtccgttca tatcctttgc ccaccttttg atggggttgt ttttttcttg taaatttgtt 91500
taaattcttt gtatattctg gatattagcc ctttgtcaga tgggtagatt gcaaaaattt 91560
tctcccattc tgtaggtttc ctgttcactc tgatggtagt ttcttttgct ctgcagaagc 91620
tctttagttt aattagatcc catttgtcaa ttttggcttt tgttgccatt gcttttgctg 91680
ttttagtcgt gaagtccttg cccatgccta tgtcctgaat gatattgcct aatctatttt 91740
tgaaagtgtg cataaggctt aatgctagaa attggtaaca ttttgatcaa attcatgact 91800
gcattcttga gaataagcgt gtccaggcat attccctatt tgattccaga attataccac 91860
taagatttgt gctcagtttt cattttatca attcttttcg tttttttttt ggatacaggg 91920
tcttgctctg tcacccaggc tggagtgtag tggcgcagtc ttggctcact ctagcctcga 91980
cttcccaggc tcagctgatc ctcctgcctc agcctcctga gtagctagga ccacaggcac 92040
gtgccaccac acctacctaa tttttgtatt ttttgtggag atgaggtttt gtcatgttgc 92100
ctgggctggt cttgaactcc tgagctcaag cagtccaccc tccttggcct cccaaagtgc 92160
tgggattaca ggggtgagcc attgcacctg gtgatttttt cagtttcata tgagaaaata 92220
tacttctatg atagtgacag gtacaaatat tttacatagc tataaatttc agtttagatt 92280
atatttgtca attggattcc atggatatta tctttccttt ctgtttaaac tatttaaagc 92340
taagctaccc tcatatcact tgttattgat aattcagatg tatcctcaga tttgtactcc 92400
aaaggcaact tcagaaactt aaaacatggt tcagactctt gtgcatctat aaaattcttt 92460
tgaaaaatag aaagccagag gttgcaaggc tttagttagg agtaaattca ggagaaccat 92520
tgtacaacat ggtgacaata gttaacaata tatcgtattc ttgaaaaatg ctaagagagt 92580
ggatgtaaaa gtgttcttat cacaaaaata actatgtgag gtaatgcata catcaattaa 92640
ctagatttag tcattctgca atgtatatag gcttctaaac attatgttgt atgtgataaa 92700
tacaattcta tcttctagtt aaaaaaaatg agaaattatt tttgccaaat agccctaata 92760
atcaccagat agtcaccaaa taccttgcag caatataaaa taaatgcaga ttttggttat 92820
catttgtatc attcatatat ccaatttgac tggcaattct tccttttctg aacaaatttc 92880
aaccccttaa ttttctccag tttcatggtg tgtctatgcc ttgctacaac ctgctgaatc 92940
tcatacatat ttgactggtt aggtcttcca gagccctgca gttccaaaaa agatttaatg 93000
aggcacttgt ttgtgaattc actagccagt catttgatta aattaaaatg aaatttctgt 93060
gtttcgcttt tctatatttt aaatatcccc ctattttcta tatgtattca agtgacttgg 93120
aaaggttata gcaccatctc ttaatgaagg atttttgtgg aaagctaact cacacctctc 93180
ttctttgact tgaataaact gttgtaatct acttttgtct gttttgttca atataggtaa 93240
attatctgtg caagcgaact cacgtttaga gagaatttct cagttttctt cccgtagatt 93300
ctcagtccta ttattagttc atatgtgttg gttaagtggt aaaacaaggc ttgggctgaa 93360
agccttattt tccacatata cagagccata gcttatactg gagaaaagaa aagtcacctt 93420
aatcttttgg agggaaatga tgaaatctgg aatagtcata accaaacaat gtatatttaa 93480
caagatagct caaagacttt ctatttttat aaatttaaca ttcttttaga atgattgaat 93540
ttagaattat tgaaaatagc ataaattctt aaagaattta aatgtttgtg ccatttgtta 93600
agcttctaca atatgctgga tctgctcttg tgactttgca tatatcatct ttaatcttca 93660
caacgaatct gcaaaataaa tattatccac ataagataga tgagaaaggt gaagctccag 93720
aggttaagta tcttggctaa tgccacacag ctaatatatg atggagtgag gatttgaagc 93780
tacatttctg aatataatgc tctttcttac ttctggacat ttgatagtat ttgttgaatg 93840
cttgaaagat gagaagacta gcacacttgt ttactgggtc tatatttcct gcctgtctaa 93900
caccgaattt tttttttttc tggaagaaaa cttttggctt cctaataggt gcattttttt 93960
ctgttaataa ttttattttt tcaacacttt taaagaaaaa ttgaataggt agtatggaat 94020
gctcctatag gtgcctctct ttccctgcca tagcttctcc tattatttac atcttatatt 94080
agtacggtat gtttgttaca attaatgaac caatattaat acattattat tgaagtccat 94140
ggtttgtttt tatctaatgt cccttttctt ttccaggatg ccatccagga caccatatta 94200
catttacttg tcatgtctcc ttagggtcct cttgctgtga cagtttctca gactttattt 94260
tttttcccct aaagcctttc cactctacct tactgctgcc tcagatttac catttgcatt 94320
taagtacaat tgtaatttgt tactgattac cttgagattt tttggtggtg gtttttcaaa 94380
gagctttgct actacatacc ttgacatttt taaggaatcc aggtgtccag gttattatgt 94440
attctaggat gttcctctgt tagaatatat gtagtgtttt tctcatgatt agcctgggat 94500
atgggttatt gggaggaaga ccatgcaagt gtgctgccat tttcaccaca tcatatcctg 94560
ggtatgtatt gtcaacacga tttatgactg ttggtgttaa cctgaaacct ggtagaagta 94620
gtgcttgtca gttttctcca ctgtgaagtt tactcttttc ttccctccct tccatactgg 94680
aagtcattac atgagcttgc actgaaggag tcggggggtt gtgctccccc ttttttagag 94740
tggtgtatct acataattta tttggaattc ttctacacag gagatttgtg tcttctcctg 94800
catttattaa tttattcaat catttatatc actgtggaga catagatatt tattttatac 94860
tttgagttat aattcaatac tactttattt attttgttac ttaaattgat ctagctttgg 94920
ccattgggag atgaccctgt cagtcacttc cgtgtcagtc gctcctgtgt ccctttgcat 94980
acctcagtgt tttttttttt gaccactttt tggcactgca agatgctctg ggctcatctt 95040
gcatatttcc tgccccagtc ttaaaatcag ccatttctcc aagaagcctt gattcctttt 95100
attgaagagt agtgttagaa accaacatct ggtgctgggt gtgcttatta ctgaggtgtc 95160
atttctttta aactctccca gtgtcaggga aaaatgtgta tatactaatc tttagatata 95220
atacatatat aaaaatattt ctatatgtgt ctctatgaag ttaaacatga gttcttactg 95280
atgtttccag ctctaattta ttacaactcc taatatttaa gtgattttaa gaacactttt 95340
taagtttatg gcagctttat tctttacctt tttcttttgt ttagctttgc ttctatattt 95400
aggtggactc tattcactct cagttgtgtc gtataaggac ttctcaattc ttgaattgtt 95460
ttaattcttc cttgggctgc ctaatttttt atacacaagt ttcccctacc ccaaggaaaa 95520
aattcttcat ggttactata ttttctaatt tcttacttgt atgtttgaaa attgtattaa 95580
aaaaactaag gattggatcc tatcagtgag atatttgaga attatatctg ttcacctttt 95640
aattttcaca gtattgcttt ctgattttgg atggatatat tagacaaagg ggatggagag 95700
tagtgttgga ttgatactga aatgttttta gttttgccta ctctgcttct agatgacagt 95760
gttcaaaagc ttatagtttc agtttgggtc ccttcccaag ttaagaacac tgttgatgtt 95820
gttctttgct tattttattt cttgtgttta gttcattggg ttcagagaga tacaggttca 95880
gtgattctgt attgttctgc catctttaca cttgatctta gccaaaaggc cgagaagtga 95940
tattctgcta tcttttactt tgaccaatat tttctttccc ctaaagcctt tccactctac 96000
tgcctcctca agtttaccat ttacatttaa gtacagttgt aatatctagt tgctatcttt 96060
gctgttatct agtacaactc tcattttata gatgaaggag cttaattgat tatcatttgc 96120
attagaaact gacacactaa ttttttcccc ctaattgtct caaacaaaag ggaaactact 96180
tttctttgtc aggctttttt caataaagta tttttatgtc taagcatatt tgtggtgact 96240
aattatattg tttttgtatg gattttttgg ggaagaaaaa tgtacattaa aagtcttctg 96300
ttagtaagat aatgaaagat aaatctttgt atttccaagg caaagcaagc ataacttctc 96360
ttcaaacaga ttgttttggt caaatttggt tgttgcagaa agcccattca tttttttagt 96420
aacaacagat aaagagctta actaaagttg tgatgaaaat acatatactt atttgagaag 96480
actggtaatt tttgaaaagt cttaagataa tcagtataaa agccaacttt ttaagcaaag 96540
aaagtaaact attaagttta tgcaatgatt cattttggat atttggaata ggatatgaca 96600
ttattatata acatagtttg tcagcttaga cttattttgt agcacttaag tgattcagtg 96660
ttttaaggga tttaaaatgt gatcctttac aatacacttt cccaaaatca gtgaggaaag 96720
gaaattgcta tgttatgttc aaatcttgtt aaaatcttat tgctatagtt tacagaagag 96780
gtatagtgat tttaaaaata tttatgcagt gcaaatgctt gctgtatatg ttttacaaag 96840
tgaatcaaag ttggagggta gttatttaat aggggaatat ttgttggttc ctaagtcatg 96900
gtttctgaaa taaagaaaaa tagcagtatg cagtagacct ttattttatt atcagtctat 96960
tttgatgtgc ttttaatagc actggtgatt aaagttgcag agtctggagc cagactgtta 97020
agttcaaatc agtgttccac tatttgtaat gtttggcctt ggcttactta agcccaggtt 97080
cttcatctgt aaagtggggc taatcatgta cttgccttgt agaatcctta taaagattga 97140
gtgaatttat gcatgtaaat ctctttgatc ctggcacaga gaatatgctc aataaatgat 97200
agctatggtt atattttttt aaaggagtat gggtttatta tttatgtgtt ttctttgaag 97260
gactgataac ctttgacatg aggcttattg tagagttctt ttcttggtat tagatcttgt 97320
gcagtttggg agagtttact atttaattca agcagtttgt tttcaaaatt tatgagcaga 97380
catttggtgg tttggagtga ctacaaggat gaggaagtat aaacagagat aggtagtcag 97440
ttcttggtag ataccttttt ctttttttaa aaacacttga gaaatactat ttagaactga 97500
gacccatgta gacagccacc accatactgt tcatcattag ccataccatt ccccatcatt 97560
agttaaggaa atgcattcga tttttaagtt ttttcccccc tttctgtgct gtcttttttt 97620
ctctcctgag gatgcttctt gtatctttgc ttctgacttg agggcaggcc aggcagataa 97680
taagtgtata gctgctgcac cattttatat acatgtgaag gtcctgcttt tgatgtaatt 97740
gggtctctgc ttctcttcac cttaatgttc ttaacaactt gctaccacca tgagccccac 97800
aaactcatat gacttggtat gatctcactg attagatctg atggtagaat taatttaaaa 97860
ccacccattt ttacaattga cgagagtagt gggaaaaaaa aactgtggca tgaacattga 97920
gaagcatttc aaattccatg tagatggcat gctactcaat tttttacata agcttctgta 97980
tttaattttg tataagaaca aatgcctgcg gagtatatag aggccaaatt tttgtttgtt 98040
tgagtttact atattcacat caacaactgg aattagggta ggctgtagaa tagaaatatc 98100
ctcttggtaa taatgcagca aaattctgtc tgtaaggctt ttatgctttt tggcacaggt 98160
catatgattg tggtttagga aatatactca cctcctctct tcatctgttt tctgagtcct 98220
aatgtgattt cccagaatac ctagcacata taattcaaaa agacaatatt tgtctttgct 98280
ctggttgcag taagataaaa ctctgaagaa ttctgggtta agatgttaca gaaagttaat 98340
ttccaggaaa aattactcta ttggagaagg tatagctctt tctaagagta ttccatagat 98400
gagactggta tctgtttttg taactctatt cttacatatt ctattccact tcagcaaact 98460
tcttttgagt agatgataaa actcttaaat tttttacctg ggtgctctta tctatcaaat 98520
ataaaaaagc ctttagagaa aaatgtattg gtgacttcaa atagtttagt caatacgcat 98580
ccattttgca tttttggtgt tggttagttg aagaagaatt tcaatggtgt gtgttagtct 98640
tgtgtggctc tcatagttac cgcctgggca gtatgtggaa ttcactaaat gaattgaaaa 98700
gttctgaata tctctttaac ttatgacaaa tccctgaaaa tcagtgcttt ctgatgagtc 98760
aaactatata ggtagtatat aaaaagtcaa catatgctat acgggacagt ttaataagca 98820
tttagagtat ttggctcaaa tgtgttctgt gtactttaat cctcttctgt gatttgtaat 98880
atttagatcc attttcccta ttgggttagg tggaaataag gcattgacag tcaatgaatt 98940
gcttttgggt agagctcaac ataaaacaga ttttgctgta gtaaagatta tagattatca 99000
tcttagaaga aaatgtagac tgttatgtga aaaaaaatgg ccagattttg taagattgta 99060
attgggaaac tctgcacccc tttgaccaat tttaacttat tactctttct tactagctta 99120
ggcttaaaac aataatgctg tctgcaagat ctgcctttct tgtgatcccc ccatccccag 99180
ccattaaatt ctaactagga tctatgtttt cttctctgga gttacttttg gtgggtggtg 99240
gagggtccca cggatgtgaa taagttcact ttcccctact tcttttattt tgtaattatt 99300
ttacttctaa gttcactttt gttacatttt tttcttcctt gactcactga gttagagtta 99360
gtctcattgt tcagaagccc tcttccttta taacttcttt ttttccaatg cctctgttat 99420
atgcagaact taccttcatt gcctcataaa tattggtgtt aggagacatc atccctcatc 99480
tgtctcattt tgcaaataag cttcctgcta gaaagttggc tagatggaag cagagacctt 99540
cactaaaacc ttgatcctcc tctgttttcc cagcacatag taggtactca aaaatacatg 99600
ttgaatgaga gaaaggaaat gagatccaga gaggctaagt gaagtaatat atgagaatat 99660
ataacatgta atgtcatgaa tatatgttat gaaagtacta aaaggctcca gaaatgttag 99720
ttatttgttg ttagtattag tgtaatagta aattaccatt aataatattt taaaacttat 99780
tactagatga tatagagata cttttcattg tatgtaacag aatacccaat cgatagtgac 99840
ttaaactaat gaggattcat ttttacattt tgagggtaga tatctgccaa cattgactta 99900
gctgctctgt aatggcaggg ctattgttct gtaattttct tggcctttcc ttacttgttg 99960
caagatggct gcttctgctg taggcatcac ctttctgttt gaagcaccag gaaagggcat 100020
ggatcaacaa cagcatctct gttttaccag ggaaccaaaa gccttcctgg aagctgtgta 100080
gtacaacagt cttcccctta agtcttatgg ccagactgtt tcacatggcc acctctatct 100140
ggaaggaagg ctgtgaaagg gagttggctt ttctagcctc tactgtagac ggggaaggga 100200
gaaggagttt ggaaatacat tctgggtttg ctgcaaaatc tacctcttcg gctagcccaa 100260
atccgaatat atccttctta tgaatacatt ttatagagaa ttcagtcacc atctctcccg 100320
gggagatgat acctgttctt atacataatt gcatctactt caaagtccag aatgtctgct 100380
tgatacatag ttttctccat caggtctagt tgtagcttgt tgtcctctgg tgacctagaa 100440
ctaagaagac aaatatatac ttccagcaca tcaaaaacta ctagataaag gaatgagata 100500
actcaaataa atcttttcct ttagaaaagg ggagaattag aaactcaaaa tagtcattgg 100560
tccacaaata taccatactc tgtaggataa gaatattgaa gatttcctgt tgtggcagtg 100620
agtgaaattc cttaaacaat ctgctatact tatttatgat ccctgggagg tttcccttgg 100680
tcacatctga ccagaaagta ttgattggtg tagctgtata ataagtcagg tagaccagtt 100740
ccttccacat tattcttttc cgaattgttt tggctatttt aaggacttcc cctttccata 100800
taaattttag aataagtttg tttatgccta ctgagaacct tgctaggatt tttgatagga 100860
attacgttaa agttatatat caatttgaga agaattgata tctttactat gttgagtgtt 100920
ccaatccatg aatttgataa gttcctctga taatttggag agactttaat tttttcaaca 100980
caattttata atttttagca tgtagatcct gtgtatgttt tgttagctat acacctaagt 101040
atttcatttt ctttggcaca aagaagtata attggaatgg catttgcctt ttaaaatttg 101100
tttcatttgc tgttagtata caaaggtcat gatttttgtt tgtcaatctt aaattctgtg 101160
accttgcaga agtcacttta ttctaggagt tttttgttgt tgttgttgta aattccttgg 101220
gattttctag atagacaatc atattatctg tgaaaagaga cagttttatt tgttcatttc 101280
caatctatat gcattttatt tccttttctt gcttcatttt cctggctata atttcttgta 101340
ctatgttgag tatgagtggt gagggtggtc atccctacct tgttcctaat cttagggaga 101400
aagaatttgt ctttcaatga gtaagtctga tgttacctct gggatttttt ggtagatgct 101460
ctttatgtgt ttgaggtaaa tcttgtctag ttctagtttt tttgagtgtt tttaccttga 101520
ataggtgttg gatactttgt agatattaaa aatactatga agggagactg gattattctt 101580
ttttagctgg aaatagagta gtatgtgaat tagaatgata aagtctgact gttgtctcag 101640
gcatacaata cttaaggcac caaagacatt aactggctct gaatcaactt catgtatttc 101700
tctatttagg gaaagattta ttaacataat tacatgtgac tattctttct tattttggct 101760
tttaaaatag atgttccttt aacataaaca tttttaaaaa gcatactttg tcagtatctt 101820
tgtcggttct gccactgata ttgttcccat aaaaccaacc cttattttac tttcagggaa 101880
aaaaaaaata cttcatgtgg ttaaagaatc atgttgtatg ttttatagtg ccttccatgc 101940
tcttgcaatt ttccagtgac agctatatat tcttacattg cagagcagcc tgcctcttta 102000
gtcttcgttt caaaataaaa ccaaaaagaa gaaaaaatta cagaaatgtt gttaagaata 102060
gtacaaaacc cttttttttt tgttttttgc cttctgagtc atttgatagt gagctgttta 102120
cttgatgccc catcatcccc aaatacttta ttgtgtgttt cctataaaat tattctccta 102180
cgcaactacg acaaagccat cagcaacagg gaaataacac ttaggcatta ccaccaccta 102240
atcctcagac ttcattcatc ttattccctt aatgtccttt atggcaaaag aaaggattcc 102300
ctttgaaatg tctctttaag atttctttaa tctggactag tttctctctc tttacttgcc 102360
tttatatctt tgacattttt tgaagagtac aggccagtta cttgagtgat tgtgtcttga 102420
tttggctttc tctgatgttt tctcatgagt aggttcaagt tatacatctt tggcgggaag 102480
atcttcagaa atgatgcttt ttttctctct cagtgcatct tatcaagtaa cacctgactt 102540
tgatttgtcc tattattggt gatgttaact ttgatcagtt gattaaggtg gtgtctgcca 102600
ggtttctcca ctgtaaggtt attttttcct cttcgcagtt aaatgtattt tgtgaggagg 102660
ttctttgagg ctatgtaaat ttccccttcc tcttcaaact ttcacccctc ttgaattcct 102720
gtgctctgca ccttgctgtc cctctatagg aatgcccttt tcaccctttc tggttctaac 102780
acccagcaaa gaggctcttc tgaacctgca caggccatcc tgctgcctgg gagtgatacc 102840
tgcctcactc catttctgct ctcctgccca atatggatgc ccttttcacc tcctggtctt 102900
tgacacccca ggccaggctg ccttgtcatg cagatgccca tcccattgtg ccgcagctct 102960
gtcaccctgt gctggttgcc ccctgtagag atgccccatg ttcttcttgg gctctgagac 103020
cctgtgctgg gctgcctgtc ttggtggatg ctcttaccct gctcatactt ttttattttt 103080
tatttttgag atagagtctt gccctgttgc caggctggag tgcagcggca cgatcttgga 103140
tcactgcagc ctctgcctcc tggattcaag cgattctctt ctctcagcct ccttagtagc 103200
tgggactatg ggcacccacc accacacctg gctaattttt gtatttttag gtagagattt 103260
gtatttttag atagagtttc accatgttgg ccatgctggt ctcaaactcc tgacctcaag 103320
tgatctgcct gcctcggcct cccaaagtgc tgggattaca ggtgtgagcc accacgccac 103380
gtccctgctc agactttgat accttatgcc aaactgcccc tctttgtggc cagcccagcc 103440
ctccttgtgt ctctggcatt gatagcccat attgattcac cctcccatac agaggccttc 103500
ctcatcctgt gtgtgccctg acaccctctt ccccttggat gggctctgat cacgtttcct 103560
aaggctgctc tctgccttcc cccagctcag ccacctgctt ttatcagtcc cacctattgt 103620
ctttatgctg aatttgtcta gaagggtagg gaggtaaaag gaactgcagt ccctcttggt 103680
attcttaatc agccccctgt tgtttgcttt ttcacctttt tttttttcta ccttttcatg 103740
aagagtggtt agagataatt tataacattt gaaatgtaaa attattttct tagaaaaaac 103800
caggtgacta taatcctttg aataaatgtg caaagcctga tgattttgga tattttcaaa 103860
gaaataatcc ctgaatattg gagtgaagac atcccatatc acagtaccta atgtcagatg 103920
gcaaatagct agcatggaat cagcttgctt cagttcagcc tgtagagcca aagatccatc 103980
ctggcactgc cttctggttt ccagcttaag acacctcttt gcaagtcaca acatctcttt 104040
ctatttctgt ttccgcatcc ataagatggg ggttataaat acttaggtca tggggttgtt 104100
ggggagctca gatgagataa tgcatttgaa aatgcaagtg ggaaaatgct atgcaaatgt 104160
ggttatttag ttctaattta ttacctagtc aaaattgaaa tgtataggaa attaagtcaa 104220
gcattttaat tgcacttgtg atttgtaggc aaattccctc tgagtcataa ggcagataaa 104280
aggtcggcat ggttttgttt ttgctgccac ttttaagtcc attgtgttgt cactattact 104340
caaccaggta aacactcctc ttttgaagtt ctgcccacct gttacgcctc tgtgacatac 104400
tgccaccttc tagatgattt ttacctctgg tgtacaactg tcctccctac cccagtgcct 104460
gttttctgtc atctttggtg acatcagctt cattggcctt aacatgagtt ctttcatgca 104520
tctacaatag cttgtggcac agttctgctt gcatatatca ctggttaggg atctcaaagt 104580
gaacatgtct aatgatcaag ttacactctt ctcccctgac ctgctcctcc ctagagcttc 104640
catttatgtt actggtgtca ctctttgtat tagccttgta gattggaaac ttttaactct 104700
tccctctgtc atctcccatg tctagtcagt cagtaagttc ttttattttt gcaaccatct 104760
cttctccact tgagcatcta tttccccata attaatggtg tattccttta aatctaatct 104820
ctgaactctt aaaatagcct tcttcaaggc aaatgttaga acctcagctt gccaggtccc 104880
agactggtcc tctgctgaat aattggatga aaaagaccat ggggcctaag tcacttctct 104940
gagtcagccc ttccggatga accagtcaga tgttacagga catgtctcca gagacctgta 105000
aaaacctcta gatggtaggg ttcaggcttg gggaagaaga cttaccatgt taggcccaaa 105060
ctcgtgcctg gaagcatcta gaatctgaaa ggacctgttc tgtatagcat gaattccagt 105120
atctaaatat agcaactgta aacattttgg tgcattttct tttatttttt cttttgcatg 105180
tctataatta tactgtatgt gttcattcat cacatctgtt aaatgaaaac attttaatga 105240
tattttagta agtgcatttt taaaattggt gtgtaacacg gatgcccctt gacttaggat 105300
ggggttacat cctggcaaag tgaaaattca tatgttggaa atgtgcttaa tacacccaac 105360
ctactgaaca tcatagctta gcccatcctc tcttaaatgt gcttgtacac ttaaattagc 105420
ctataattgg gcaaaatcat ctggcaaaac agtccactgt agattattag ttgtttacct 105480
tcttgatcgt gtggctgact gggagcagtg gttcattgcc cagcatctcg agagagcgtt 105540
gtagtgcata tcactggcct gaaaaagatg aaaattcaaa atttgacaca tgggctctac 105600
tgaatgtata ttacttttgc accattgtaa ggttgaaaaa ttataagtcg aatcattgta 105660
agttggagac catctttgct tcagagaatg aaagattgct atccatgttt ctgttagaac 105720
aagaagatcc cttccaagaa ataccagact ctctgtcaga gcttaacccc agcttctgct 105780
gagagtgttt gtttagtgtt aatttttgat tgctgacaac tagtctgttt ttctcctacc 105840
tcggtcatga tgcctactca ttaagttacc tttctgcttt cttggtctcg cttatttgtt 105900
cattcatgta tttattcttt tattattcat tctccaataa taatagcata gtctcttcta 105960
tctcctgagt ttggctctga agacttaaag attgcttagt tcatcgaggg actatttatt 106020
tttgaatcgt tgccaggatg taccacgggc ccatctgtgg gcacatagaa agcagtgcct 106080
tcctttctga agagagtaga gaaagcttcc tgggggaggt gacgtgtaca ctgctctatg 106140
aaagatgagt agcaggagtt ggttagggca gacaggtgga agatacattt aggcagtggg 106200
cacagaaatg agaacagtgg tgatggtgtg gctccagctt acctctaggt ttttgttttt 106260
gtttttgttt tttaacagct gtattgagat aataattcct atgccatgaa attcactcat 106320
ttgaaataca cagttttcat agagtcaatt taaaacattt tcaataccac acaaagacac 106380
tccacatcct ttagctgagc ccactcagtc ctcctatccc tctcagccct aggcaatcac 106440
cagtctactt tctgtgctgt ggatttgtct cttctggaca tgcgtttgag gtagaatcag 106500
acaacctatg ttcttttgtg cctgacctct ttcacttaca taatgttttc aagattcgcc 106560
tatgttgtgt gtatcagcac ttcatttctt ttgattgctg aataatattc cattgcataa 106620
atataccaca ttttatttat ccatttatca cttgatggac attgggttgt ttctgctttt 106680
tggctattag gaataatgct gttattaaca ttgatgtaca agtttttatg tgaacacgtt 106740
ttcatttttc tggggtatat acttaggaga ggaattgcta gattatatgt taactctctg 106800
tttaacaatt tgaggaattg ccagattatt ttccaaagca gccaaaccat tttacatttt 106860
gattaggagc atatgagggt tccagttcac gtccttgcca acagttacta cttttaaaaa 106920
agtatagcca tcctagtaga tgtgaagtgc tatctcattg tggttttaat ttgtatttcc 106980
ccaatggcta gtgatgttga gcctcttttc atgtgattgt tggctattta tatgtctttg 107040
gagaaatgtc tattcagatc tcttgcccat ttttaaattg gattgtcttt ttgttattga 107100
gttgtgatag tattttcttt ttaattatga aattggtagt tgttttgacc aagtaagttt 107160
gctaggtcaa aatctagaag tggttcttat attggagata atattctgca gattaatcta 107220
aagatctctt ttaagatgtc tctttaaaat tctatactct agaattggtg ccgatcctgg 107280
aaatgtacca ttctgagcag atgacttgtg ttattgattt gtgccaaaca actaagggtt 107340
gtgtataatc tgaatggaaa gcagatgttt tggcagtttg ctagtgagtg gttttctctt 107400
ggttagggcc tgctccttta ctaaatagaa ctattttcag tgtatcactt ttcaaatatt 107460
tgaataagga aggagatttt tgggggacat acaaatttat ggatacaatt gaattttttg 107520
aacaacttta ttattttatg tgtagtaaat tctcaataac gacttatttt ctttttcatc 107580
aaaataaatg tttaactagc tgcaaatagt atgtatctta ttgacccagt tttcaattat 107640
gcatatgtac agattatcta tgactattaa aacaaactct tgctttctag tttgttgcta 107700
gttccctgaa ttgccttaat ggaaaccata gggagaaagg agattattgc aatggtccag 107760
gtgtgacatg atggcttata caagagtggt ggtggtagta gtgataagta gcaggtgtat 107820
ttcacattat tttggagata aaatacattg ggaattgttg gatttgccat ggaagatggt 107880
gaggggaaag gaatgattaa acatgattcc agcaaggtgt ggtggctcat gcctgtaatc 107940
ccagcacttt gggaggacga ggcaggagga tcacctgagg tcaggagttt gagaccagcc 108000
tgaccaacat ggtgaaaccc cgtctctact aaaaatacaa aattagccag gtatggtggc 108060
tcatgcctgt aatcccagct acttgggagg ctgaggcagg agaatcgctt gaacctggga 108120
ggtagaggtt gcagtgagcc aagactgcac cattgtactc cagtctgggc aacaagagca 108180
aaaccccgtc tcaaaaaaaa aaaaaaaaaa aaagaagaaa gaaaaaaagg atgattccta 108240
gggttctggt ttcaccattt ggggagatgt gccatttatg gaactgtaga acactggaag 108300
aagtgaggtt tagtcatttg cttattaggt ctcaaatcca attctctgac cctctcagtc 108360
ctgttttgta ttgcatttca taggtatcct taccatttgg cttttgtgag tagatctgga 108420
caattggagg cactgatgga agactggagg gtaggagtag ggagaaggta gggtattttt 108480
ctccctccct ttttactccg ggtggtgtcc caggaagtgg cagtttcatt cctgtggctt 108540
cagctgtcag ggagctcctt ccttcctttg tagtccctgg tcccaaaggg caactcctgc 108600
tattctagcc cctgtcaaat gatcccagct attgggctct tataatacag gttgagtatt 108660
ttttattcaa atgcttagga ccagaagtgt ttttgatttt ggattttttt tttccagatt 108720
ttggaatatt tgcattatac acttactggt tgagcgtccc taatccaaag atccaaaatg 108780
ttgaatttcc tttgagcatc ctgcggttac tcaaaaagtt tcagattttg gagcattttg 108840
aatttcagag ttttggatta gggatactca acctgtacca cctcttctag tgggtctccc 108900
cagcccttag gctggtagta gcttcctgct gggaaaaggg aggaaagatt ttcctctatc 108960
ctcttagggt ctttggctga gtctgaaaat caaattgcca aagacagatt aacagaagaa 109020
aaacattcaa atttatttaa gttttatgtg acatgggaac tttcacaagg aaatgaagac 109080
ccaaagaaac aggtaaactt tagtattttt tatagtaggt ttgaagaaga gtggagagcc 109140
atggagaaat atggtagggt aaaagagtgt gatctaatgg tagtaaactg ggggaaacat 109200
agtaggacct gattttgcca gattcttctg tgtcccctcg tcttcagaga taaggatgtt 109260
cctttcctct gggtataggg agggcatgtc tcagatgagg gtcttacaac ctgcttcagg 109320
ggaaggtcag aaattttctt ctaggtttta tgacttgttt caggggagac aagagggaca 109380
aggtcagaga gaccttcctg cttctgctat ttttcagatc tcttccattt aaaatactca 109440
ctatgtcgag gtggtatatt ttggggtagt gtgctctgag ccccatcact gctgttgttc 109500
attttggtgt tccttcccat tccctgtttg gagtcttgga tcttctattg ccatgtaacc 109560
acatttctct gttaatttgt atctgtttta atgcttagag tggtttcttt tttcctaact 109620
agaacctgat gaatacaggg tgaaaatttg agagttcaat tttggaaatt atttgtgtca 109680
agtttagtat ttctgtgatg cattccagtg atgttcttag gtgagttgga tagtgagctc 109740
agatgcgtgt gtgaactaga agtataaatt tggctatcat ctcagccaaa tggtagtatt 109800
tcaaaccatg ggagggatgg atgggatcat ctagtacaca gaagagaagg gaatccaaat 109860
ctaggtcctc tgaaactccg agtcctgaga ggtgggatgg gggagggaga tggataaagt 109920
agaaaggcca gggagagtga tgctgttgat aacataaaga aaggattgtt ggcggggtga 109980
agtggcccat gcctgtaatc ccacactttg ggaggccgag gcgggtggat catctgaggt 110040
caggagttcg agaccagcct ggccaacatg gtgaaacccc gtctctacta aaaatacaaa 110100
aattagccag gtatggtggt gtatgcctgt aatcccagct actcgggagg ctgagactgg 110160
agaattgctt gaacccggga agcagaagtt gcagtgagcg gaaatcgcac cactgcactc 110220
cagcctgggt gacagagtga gactccgtct caaaaaaaaa aaaaaaagaa aaaagaaaaa 110280
agaaaggact gtttcactgc tttgtgcatt ctgccaaatg tttatggtgt cagttacctt 110340
gatgaaagca gttttaggga cacggtgggt agttgagggt cttcaccaaa atgaagtggt 110400
agatgagtga atgtgaggtg acagagtata gctcgtgcat agagagagtt ctttcgagat 110460
gtttgataat gaagggggag taaacaaatg aggaaggccg ggcacagtgg ctcacacctg 110520
taatctcagc actctgggag gccgaggcgg gaggatcact tgaggtcagg agttcaagac 110580
cagcctggcc aatatggtga aaccccacct caactaaaaa tacaaaaatt agccgggtgt 110640
ggtggcgggt gcctgtagtc ccacctactc gggaggctga ggcaggagaa tcgcttgaac 110700
ctgggaggca gaggttgcac tgagccaaga ctgtgccact gcactccagc ctgggcaaca 110760
gagcgaaact ctgtctcaac aacaacaaaa aagaaatgag gagatagcag gaagatgtat 110820
taagaaaagc tacagttttt atgattgcct tttactttaa aaatatttaa tttttagaat 110880
agtttctaaa tgaaagtaga agtcatacaa ggaaaagttt tcctcttctt tcccattaac 110940
tcttttttac tcccttctcc cagccccagc atatagccac tgctgtgtgt ttctggtata 111000
catccaaaaa aattttatgt aaataccagc aaatgtgatt atatattcta gattttcccc 111060
ttcttatccc tagggatagc ctactgtata tatttttatt gtcttatttt tttttctctg 111120
aataatatga cctggagatc tttctgcatt ggaacttaga ggttattcat ttgtttatag 111180
gaatatttgt tctgaaggta ctaaaatatt tgtaaccagt cattttttac gattacaaat 111240
aatacctgta tgtttgcagt aggctaactt cttaaaaggt gaactgctag gtctgagaat 111300
atgcacctgc catttcagta gatactgtca tattacctcc ttaagtggcg atctggtatc 111360
tatgcccacc agcaaagcat gaagagtact gttttctcac agttacatca gtatagtgtg 111420
ctatcaaact tttggatttt tgacagtcta gtaagtgaaa aatagtattt tttttaaaaa 111480
gtgcagtttt agttttcatt tgttaagaat gaagttgaac atctctttgt atgttcaaga 111540
accatatgtg tttcattttt tcatgaactc tcagttcata tttgttgccc gtttttttct 111600
attagctttg gtcttttaag tttttgagtt ttgtatgtta ggcctaaggg cctgacagct 111660
cactgtggct gtaacattgc ttgtaagatg ggcctggtga gcagtagttt tggattgggg 111720
catggtggct ggatcgtgaa gggccctgga aagtccattt ggtgtttata taggctcttg 111780
gtttataaaa ctttttttga cttacagaaa tagtatctta ttagagattc tttttttttt 111840
tttttgagac ggagtctcac tctgtctcca ggctggagtg cagtggcgcg atctcagctc 111900
actgcaacct ccaactccct ggttcaagcg attctcctcc caagtatctg ggattacaaa 111960
cgcgcgccac cataagtatc tgggattaca aacacgcgcc accatgccca gctaattttt 112020
gtatttttag tagagacggg gtttcaccat gttggtcatg atggtctcga tctcttgaca 112080
tcatgatccg cctgcctcgg cctcctaaag tgctaggatt ataggcatga accaccgcgc 112140
ccggcctagg gattctctag atgcagtaac ttctgaaata attatatgtc aaaatcaata 112200
gagcaattta atttggaatg aggatgggga atattaataa tgttcttttc tacatggtaa 112260
gtacaacgtc tgtcttgtac ctgatttgac tatgaccagg cctacttagc tatggggtaa 112320
tagccataat taattttcaa tagttactgg tagagctttg ccagctatta tttttcattt 112380
aaaagaaaaa atcgtctttc atataatgaa cagacttcaa gttgaccctt ggtttcctca 112440
tcttatttct atgaagcact cagggggact attttaagag aacaaactca ttatataagt 112500
aatctccgag aataactgct tcttcctagt agcttctatt tcctttagtc agagactcac 112560
tctacagaca ccaccaccat acaaagggcc agttcatact cttgagagtt tcagaatggg 112620
agcataggta ctctttgtca gtgtcttcct atcatttctc ctctactctt tcctcctttt 112680
cccccctttt tctgctcgtt ctcccagcgc tccaacccta gctggcttaa aggaaaatac 112740
tttatcatga atcactttta ttctttttag aaaacagatt ttgaaaacat ggttactcca 112800
tgctgagcat tgtgtaagtc caaataaaga aaaataaatg aaatgtaaag aaaaaaatta 112860
atctattgac cagaattttt attttgtttt ctttttgttt tttgagttgg agtctcgctc 112920
tgctgctcag gctggagtgc cgtggtgcga tctcggctca ctgcaacctc cgcctcccat 112980
gttcaagtga ttcttctgcc tcagcctccc gagtagctgg gattacaggc atgtgccacc 113040
acgcccaact aatttttgta tttttagtag agatggggtt tcaccatgtt ggtgaagctg 113100
gtcttgaact cctgacctcg tgatccgccc gccttggcct cccagagtgc tgggattaca 113160
ggcgcgagcc accgcgccga ccattttcat tggttttaag aagttatttt tcaagttgta 113220
ccttaagggt gctttcgaat tctcttatta aagcatactc tattctcagt ttttagtagg 113280
cagtcaatga tttgtgttac gtaaataaac cctctgggta tgggtaaacg tactgcacac 113340
ttaaaatgtt tggacttgac ttgtcttcca gagttctgtg gtaaagaaac tctcaaaaca 113400
tgctttttgg gttcaagaca atcaaactgt tttctctctt tgttttgttc ttactgaatt 113460
tttatttgta tcaacctcca taccctcctt acttaaatcc atgaatagac tccttttatt 113520
gttttgtgtt gtttcttggg ttgattttca aatggaactg ttgtgtaaga taaaggtttg 113580
ttctcctgaa tagtgttgat gtgaaccgtg ctgtctcttc tataatgcca ccattatgca 113640
gaagcctgca gggggaaggg cagattcgta gttgtgagag tctgcctgtg ctttagggta 113700
ataatcctgc attcattatt aatctccctt tgcttccatg tctcctgctc attaccaaag 113760
gccaagacat gacatgttca agatcttaga aattttgaaa atagcagaac ggctgctttt 113820
gaagcccgat tgttagtctt agaaatgaca actgtaaggc catgtaatat tacttatttt 113880
agtttttaga tattgtctac attgtttttt gatcaatagg caaagagcca ctaatgagcc 113940
attataattt ccttaaattc acagtctctg gagtcagttt gtctgggttt gtaatccctg 114000
ccaaccctct caccgtgtga ttctaggcaa gttacttttc ttttctagac cttaggttcc 114060
tcctctataa aattggaata aaaatgaagg tcaacgtggg aggattacat gaaaaaatga 114120
atgtgacagg ccaagtttat tgctggatat ataataggta ctcaataatg ttagctatta 114180
tcgttattac taatcagatt tttattaccc tggatttaaa aataattttt ctgtatataa 114240
agaaatagtt tggaaagtaa agtgtttcaa ttacaacaat agtttatttc ttttgttggt 114300
gactattatt ttcttggttc ggtgagtcca aaagctttta gcaggcaagg gaaaagtccc 114360
ttggaaaatg tatatcaggt cactgttttt ctgatggatc acagcaggaa aatagtttat 114420
gttctcacct taaaataaaa ggaagctatt gattccaagc tgtatggaaa gggaaactta 114480
aaaacattta aaaagcgttg ataagttttt taagggaaaa aagggcaatg catgctttta 114540
acaattcaaa cagtacagat agatataaat taaatattca tgtgtccctt ctcaggatta 114600
agcattaaaa aattataaaa gtagtatcca tgcaagttta aaaaaaaaga gaaagaatat 114660
aaaagatgca catgaataca atattggagc ccactgaacc tttatcttgc cttcaagggc 114720
aaaccagttt ttcatgtata cttctatcca ttttctttgc ttagaacaga atttcccttt 114780
tttctttttc ctttgtaaaa cacaaagtag atactagaca tattcttcta caccttgctt 114840
tgtttcttct gataatatca cagagattgt tacaaattgg cacgtagaga tttatgttgc 114900
tttctaaaca ggcatgatat ttcagctgca gtcccgtaat ttatttaacc agtgtcgtta 114960
tagtggatat gttggctttt tatgtttttt gccttgtcaa taatcttcaa tgagaattac 115020
ttatttatat atgtttgtgt gtgtgcgtgc gtgtgtgtac acatgtgtat gggtatgtga 115080
tgtaagtatc ctacaagcac atgtaggaga aattctgacg tgcatatata tttgctagtt 115140
ttttctactt tgccttccaa agagtttcat cagcgtagcc tgccatcagc agagggagag 115200
gttgccagtt ttcccattct tttgcttgca ctggatagta tcgcacttaa tattttctat 115260
tttgatacga gaatatagga tctcattttg ttttgcattt taaaattatg cgagatcaat 115320
gtatatcagg ataaattcct aatagatccg agatataaat gcaaaaccat atgtgtatta 115380
gaagaaaaca tgggtgaatt acttagtaat ttgggaatag aggaagtgtt ctaataatga 115440
ctcaaaaccc atcagcaata acagaaaaga gtgacacatt tgctatttta gaaataaaaa 115500
agtatgaaga aacgataaaa aagtaaaatg acaaactgga aaaaatgttg tgacttctat 115560
catagatata gatagggttg atatcctatg tggcgtatgt gtatgagtaa attaaaattt 115620
gagatgaaaa taactattag cctgatagaa aaatgggcaa aaaataggta cagatgaaaa 115680
gatgaaaaac agaaaaaaaa aaaaaaaaca ctgcgagggg cccttatgcg tgaaaaggtg 115740
cccagctcac tcataataag aaaaatgcat gttacaccct cactgttgtt tttcaccaga 115800
ttggcggaag tccaaagttt tcatagcaga ctccagggtc aaggctgcag gtaaagggca 115860
ctcacaggcc ctgctcatgg gagtgcaaag tggcacacct ttgtggagaa gaattttaca 115920
gtatgtaatg gaatttcatg agaatttatc ctctgacccc acattcccac ctttttggac 115980
tcgattccaa aagtaatctg ggaaacataa aaatgacatg tgtacattgc tactcattgc 116040
agtactgttt gtaataaaga aagactgtat gccactgccc tccagcctgg gcgacacagt 116100
gagactttgt ctcaaacaac aacaacaaca acaacaacaa caacaacaac aacaacaaca 116160
gactgtaaat aataagatat tgaaatctag attccatctg tctagcctga agtttgcagc 116220
tctccaaaat agatctgata tcccaacttt ggcattggca atatttagct tatgtgcaga 116280
tggttttcag ctgcatatca gaggttacga gcactctaag gggttctaac gctcaaatct 116340
cgaagtaggt ggagttcttt cttgggcact ctctaagttg gtgccttctt cctacctttc 116400
ccttccctgg tccactcttg tccatctgcc tggcccttct ttccttcagt gcccttctct 116460
aatattgctt ctccttttaa ggcactggag gacctggcat gaatgttcta aggcaagatc 116520
agaaggtgtt aacctcttag caggtatttg ctctgatgac cagatcatct aaaagtggca 116580
gtgtgtttgt atatttattt tattatttat tccacctcca cttatccaaa ttaatgtatg 116640
aaattgggat gtatcttaaa attgataaga acttgcattt aacttctacc cctctggaag 116700
agtttttgca gagttgatgg tgtttctcgt agttgatggc atcttagaag cccaacaatg 116760
tgtgcataag ggggtaagcc atgtggaatc accattattt attggcaaat agccatagac 116820
agttttcctt ctcacttttc atttttagtg agtttgtttt ctgtgttatt tcataaataa 116880
ggcctctgct gggtgattgt gggttggctc agagctagca aatactctaa attgagaaag 116940
gaaaagttta gggtattatc tttttgttaa caatattaac atttatacag atatctgtct 117000
tgaattctta gcatttgaca taattaatta ttccctcttt ttttcccttc tttttttgag 117060
acagaatctt gctgtgtagc ccaggctgga gtgcagtggt gcaatcacag ttcactgcaa 117120
cctctgcctc ctgggatcaa gcgaatttcc tgtctcagcc tcctgagtag ctgggactat 117180
aggcatgcgc caccatgcct ggctaatttt tgtattttta caaaatacaa agagacagga 117240
ttttgccacg ttggtcaggc tggtcttgaa ctcctgacct caagtaattt gcccgcctct 117300
gcctcctgca gtgctggaat tacaggcaca agccaccgtg tctggcctgc tttttttttt 117360
tttttaattt atttttaatt ttttaaaatc agataggctc tttaagactg taaactccct 117420
gagggtagag attctattca cagccgtatc cccagtaccc agagtacagc ctggcacgta 117480
gtgaatcctg attgcctatt gtttgttgat tgactgattt atgcctctaa gaggaactat 117540
cttttgataa tattaaataa gatgtcctaa tacaaaactg atagagttca gaaataatta 117600
agaatctcct ggccaggcgt ggtggctcac gcctttaatc ccagcacttt ggaaggctga 117660
ggtgggcgga tcacgagatc aggagattga gaccatcctg gctaacatgg tgaaaccctg 117720
tctctactaa aaatacaaaa aaaaattagc cgggtgtgat ggcgggtgcc tgtagtccca 117780
gctactcagg aggctgaggc aggagaatgg cgtgaacccg ggaggcagag cttgcagtga 117840
gccgagatct ctagcctggg caacagagcg agactccgtc tcaaaacaaa aacaaaaaca 117900
aaaacacaac aaaaaaaaga atctcctatg ttcagcggga aaggaatatt tgttgggtgg 117960
gggggttcct aaaagattat gtaatttttt ggcattttga catattcaca gaattcctat 118020
tgattgaggt tactgcatca agtatagtac agtcatgtgt tgcttaagca caggaatttg 118080
ttctgagaaa tgtgtcaggc tattttgtta tcatgcgaac atcatcaagt gtacccacac 118140
aaacctagat tgtacagcct atgacatatc taggccatta tatggtatag cctattgctc 118200
ccaggctaca cacctgtaca gcatattgcc gtattgaata ccgtaggcac tgcagagcat 118260
ctaaactaga aaaggtacag taaaaatatg gtatgaaaaa tttaaaatgg tgcacctgtc 118320
taagacactt actatgaatg gagcttgcag gactggaagt tgctctgggt gagtcagtga 118380
gtgggtggtg agtgaatgtg aaggcctggg acataccctg caccctactg tagactttat 118440
aaacactgta cacttaggct acactaaatt tatacaaaat atttttcttt cataataata 118500
aattaacctt agcttactat aatattttta ctttatgaac cttttaattt ttgaaaacgt 118560
tttcacactc ttgtaataac acttaaaaca caagtatatt gtacagctga acaaaaatat 118620
ttccttattg tttaagtgtt taaaattaaa ttttatttac ttatggccag gcgcagtggc 118680
tcacacctgt aatgctagca gtttgggagg ccgaggcagg tagatcacga ggtcaagaga 118740
ttgagaccat cctggccaac atgatgaaac cccgtctgta ctaaaaatac aaaaagttag 118800
ccaggtgtgg tggcaggtgc ctgtagtccc agctacttgg gcggctgagg caggagaatc 118860
acttgggccc gggaggcgga ggttgcagtg agccgagatt gcgccactgt actctagcct 118920
ggtgacagag tgagactctg tctcaaataa ataaataaat aaataaataa aaataaataa 118980
ataattttat tttatttatt tattttattt ttaaaatttt atttattttt actttttaaa 119040
tgtgtttgtt aaacgctaag acagaaacat acacatgagc ctaggcctac aaagggcagg 119100
atcatcaata ttactgtctt ccagcaacac atcttgtccc actggaagat cttcaggggc 119160
aataacatgt atgcagctgt catctcctat gttagcaatg ccttaccttc ttctggaata 119220
cctgcctcct ggaggacctt tctgaggcta tttcacggtt aaattttttt tttttttttt 119280
tttttttgtg agacagagtc tcactctgtc acccaggctg gagtgcagtg gtgcaatctc 119340
agctcactgc aacctctgtc ccccaggttc aagcaattct tctgcctcag cctcccgagt 119400
agctgggatt acaggcgcct gctactgcac ccggctaatt tttgtatttt tagtagaaat 119460
ggggtttcac tatcttggtc aggctggtct tgaactgctg acctcgtgat ccagccacct 119520
cggcctccca aagtgctggg attacaggtg tgagccactg cacctggcca acttttttct 119580
ttcttcttcc tttttttttt tttaaataag tagaaggagt acattaaaat agtgatgaaa 119640
agtattctat agtaaatact aagtgatagg aatttttaag ctccattata ctcttgtgta 119700
tcaactgtca tatatgtggt ccatcgttga ctgaaatgtc attatgcaac acatgttttc 119760
cctttatttt gaaaccaatg ttggcaaaat tccaaagtac aataatttgc attatttatt 119820
cattgtcata cttcttgagg ggtcctatgg taagagttat ctagtgactt gaaaatgact 119880
gggtagaaga atgtgccagg atataaagct ggcatctgat acagatagtt cagatggatt 119940
aggattatct gtcattagga ttatttattt attttgagac agagttttgc tcttgttgcc 120000
caggctggag tatggtggca cgatctcggc ttactgcaac ctccacctcc tgagttcaag 120060
caattcttct gcctcagcct cctgagtagc tgggattaca ggtgcccgcc cccacgcccg 120120
gctaattttt tgtattatta gtagagatgg ggtttcatca tgttggccag gctggtctcg 120180
aactcctaac ctcaggtgat ccacccgcct cggcctccca aagtgccggg attacaggcg 120240
tgagcacctg cacccggccc ttatttattt gttttgaaat aaactaacaa ttgctagttt 120300
ttttttatca ttagctagca gacatctgtg acattttctt gtctgcataa tatggttctc 120360
atagtattgc tttgtcattt gaaattcaac attcattcag caaatattta aggagtatct 120420
cccatgtagc aggccctgta ataagtgctg gggatatagt tacgagtaag gtgtgagtct 120480
cagtcctaaa tctttgttaa atacaacagt gaagcttgtt tcttgaagct catttctaac 120540
gatcactatg gccttccaag ctttaggaga ttggagaagc gccatatgca tgtggtccca 120600
cacattgccc ctctgtcatc ctcccctcac cctttaccta cttgcatatt gtttagtgtg 120660
ccgtgacaat ttgaatgtct actctgtgcc catcactctg ttgggatttg aacgttcaag 120720
gagcagcaga taaaaatgta ttcagtatct cttaaatagt agtaaagaca ggccaaaaaa 120780
tgaatgattt tcagtgtaca agaattgtct acacacagtc tgttatagga attcagagaa 120840
gaaagagtac taggatcaga ttgatagtag aggaatatca ctcaggtggc attgggaata 120900
atttggagga tactaacagg aggtaggtga ataatctttt ctgagttgag tagggaagtg 120960
acataattga tttatgtttt atataaggca ctctggctgc tgggtggcaa attagactat 121020
ggtctggggg tagaggaaat gaatgcaaga aatgagtcag gttagcaagg atcatagtat 121080
tcagctgata aatagtgtaa gtagtttcca tgcctttcat tgtttcaaca tttttttttt 121140
ttagctgggc atggtggtgg gcgcctgtaa tcccagctac tcaggaggct gaggcaggag 121200
aatgtcgtga acccaggaga cggaggttgc agtgagcgga gatcatgcca ctgcactcca 121260
gcctgggtga cagagcgaga ctctgtctca aaaaaaaaaa aaatattttt ttttgccatg 121320
tttataataa aatactgggg gaattttttt ttttttttta gatggagtct tgctccatcg 121380
cacaggctgg aatgcagtgg cataatgatc tcggctcact gcaacctctg cctcccgggt 121440
tcaagcaatt ctctgcctca gcctcctgaa tagctgagag tacaggcatg cccgccacca 121500
cacccggcta atttttgtat ttttagtaga gaccgggttt caccatcttg gcccaactgg 121560
tcttgaacta cggacctcat gatccaccct ccacagcctc ccaaagtgct gggattacgg 121620
gagtgagcca ctgcgcccgg tagaattttt tggcgtgtgt gccaagttac cagaaactct 121680
gatgtgctta ccagtcctcc cacctagtgc tcttatttga atctttcttc ctaggtggag 121740
aatctacaca tagacatctg tatgttctct tagttcatgt catttgatct tagcttctgt 121800
tcttatacag atactgttga aggtgctgca gttttcaatt ttggttttcc tttctttatg 121860
gtaatcctat tttaatatat aagatccttt caggcaactg aagtatgcaa gaggcaggtg 121920
gaatagtagc aactttaggt attaaaaatt catataataa atatgatggg tttggattat 121980
ttgttgcatt gccagtgagt ttattatagt atataacaat atttaatcaa aatggtgata 122040
ggaataaaag gcccactttg aggtaaatca ttaattttta aggcagacaa gttaggtttt 122100
taagaatttc tcaagctgaa gaatattcag tgtacactct gtgcctgaaa aagcttaaca 122160
cataatccac aaaggtgaaa ttgatttgat actcagagtt aagattaggg caagtttcac 122220
tttagtttgt ggaaattatt agatagtact gtcaagcatt gaaagtaaag gaagagaatg 122280
cttatcatta cattttaagt gaaaagatat agcatagatt acacattttt atatttattg 122340
ttcacaaata tttccatgtt ctagcaattt tctactgtgt tgtaattgcc tgttcatttt 122400
actccactgt ttttctctcc cctaccgcag caatcccagt gccactggat atttttgaat 122460
gattaagtgt gctacttact tcttttttgt gttcattttt ttaatagaaa atgcatccga 122520
tgcagattta tggctcctga acagttgcac tgtaaaaaac ccagctgaag accactttag 122580
aaactcaatt aagtaagtag aaattgattt tttcctattt tgtataatca aagtaagaat 122640
tgataccaaa agattagctt tttaaaaatg tcaatataga tatttagttt atattaaaaa 122700
agtatgagtt ggacttttaa gattatacta ttttgaattt gtctgataca gaatgatgct 122760
ttttttattt tttattttta aattaaattt actttaagtt ctggggtaca tgtacaggat 122820
gtgcaagttt gttacatagg caaacatgtg ccatgatggt ttgctgcacc tatcaaccca 122880
tcacctaggt attaagccct atatgcatta gctatttatc ctgatgctct cccttccccc 122940
accccctaac aggcccaact gtatgttgtt cccctccctg tgtccatgtg ttctcattgt 123000
tcagctccca cttgtaagtg agaacatgca gtgtttgttt ttctgttcct gtattagttt 123060
ggtgcggatg acggcttcca gctccatctg tgtgcttgca aaggatgtga tctcatttct 123120
ttttatggct gcatagtatt ccatggtgta tatgcaccac aatttcttta gtctgtcatt 123180
gatgggaatt tgggttgatt ccatgtcttt gctattgtga atagtgctgc aatgaacata 123240
tgtgtgcatg tatcttttta atagaatgat ttatgttcct tcggggatat acccagtaat 123300
ggcattgctg gatcaaatgg cctttctggt tctaggtctt tgagaaatcg ccacactgtc 123360
ttccacaatg gtttaattaa tttacattcc caccaacagt gtaaaagtgt tcctatttat 123420
ccacagcctc gccagcatct gttgtttgtt gacttttaat aatcgccatt ctgactggcg 123480
tgaaatggta cctcattgtg gttttggctt gcatttctct aatgatcaga gatgttgaac 123540
tttttttcat atgttttttt gctgcataaa tgtcttttga gaagtgtcca tgtgctttgc 123600
ccacttttta atggggttgt ttgttttttt tcctatatat ctgtttacat tccttataga 123660
ttctggatat cagacctttg ttatgtggat agattgcaaa aattttctcc cattctatag 123720
gttgtctgtt cactctgatg atagtttctt ttgctatgca gaagctcttt aattagattc 123780
catttgtcaa tttttgcttt tgtcgaaatt gcttttgatg ttttcatcat gaaatctttg 123840
cctgtgccta tgtcctgaat ggtattgcct aggttttctt cttgggtttt tgaagttttg 123900
ggctttacat ttaagtcttt aatccatctt gagttaattt ttgtataagg tataaggaag 123960
gggtccagtt tcagttttct gcacaaggct agccagtttt cccagcacca tttatcaagt 124020
agggaatcct ctccccattg cttgtttttg tcaggtttgt tgaagatcag atggttgtag 124080
atgtgcggtc ttatttctga gttctctatt ctgtttcctt ggtctatgta tctgttttta 124140
caccagtcct atgaagtttt ggttactgta gccttgtagt atagtttgaa gttgggtagc 124200
gcgatgcccc tagttttgtt cttttttctt aggattgtct tggctatatg ggctcttttt 124260
tggtttcata tgaattttaa agtttttttt tctaattcta tgaagaatgt caatggtagt 124320
ttaatgggaa tagtattgaa tctacaaatt actttgggca gtatggccat tttcatgata 124380
ttgattcttc ctatccatga gcatggaatg ttttaccatt tgtttgtttc ctctcttatt 124440
tccttgggca gtggtttgta gttctcctcg aagaggtcct tcacttccct tgttagctgt 124500
tttcctaggt attttattct ttttgtggca attgtaaatg ggagttcatt catgatttgg 124560
ctctcagttt gtctattgtt gctgtatagg aatgcttgtg atttttgcac attgatttta 124620
tatcctgaga gtttactgaa gttgcttatt aggttaagaa gtttttgggc tgagacgatg 124680
gggttttcta ggaataggat catgtcatct gcaaacagag acagtttgac ttcctctctt 124740
tctatttgaa tacgctttat ttttttctct tgcctgattg ccctggccag aacttccaat 124800
gctgtgttga ataggagtgg tgagagaggg catccttgtc ttgtgcccgt tttcaaaggg 124860
aatgcttcca gcttttgctc attcagtatg atatgggctg tgggtttgtc ataaatggcc 124920
cttattattt tgaggtctgt tccatcaata cttagtttat tgagagtttt taacatgaag 124980
ggatgttgaa ttttattgaa gaccttttct gtattgagaa aatcatgtgg tttttgtctt 125040
tagttctgtt tatgtgatga attatgttta ttgatttaca tatgttgaac cagcctcgca 125100
tccagggatg aagcccactt gatcatggtg gataagcttt ttgatgtgct gctggattcg 125160
gtttgcccag tattttattg aggatttttg cattgatgtt catcatggat attgacctga 125220
agttttcttt ttttgttgta tctctgccag gttttggtat caggatgatg ctggcctcat 125280
aaaatgagtt aggaagaaat cccttgtttt cagttgtttg gagtagtttc aggaggatgg 125340
ctttttgaag ggttttttgt gtgtctatct ccttaagagt aatactttaa tgggaacttt 125400
tctaataagt aatttcagta tttgtgaagt tagctaatag gtgatttttg agtatttgtt 125460
aagtgttaac tatgtgttct tctcggagaa tcaatgcata ctttgtgaag ttgcctgaca 125520
cagtccttgc ctcttttccc tgcccattag cactgttcta agtgctgcag tacctggctt 125580
tcaacggaat tgcactttgt tgaagggagg tgggcatatt aagtccttgc ttctttcttt 125640
gttccctcaa gttaacgtgg aagaagtatc cttttttcac attttctctg aatcttatct 125700
ccatggatct tcttagaaac cttcttctag aaattatttc ctcattccat atttccaacc 125760
tctccaactc ccctggaacc ttttcattgg tatttaaaca tacctgggtc taatcggccc 125820
cctgtgcccc tgttagctat tgtcctacat tttttcttgc cttcccagcc agacttttaa 125880
aaattcttct cttcttaatt tcattcttat actcattctt tttccccttt attggtttta 125940
ttgtgaaata tattaaacat acattagcgt ttataaaaca catacagttt aaataataat 126000
aaacacttaa acatctgcca cccagtgtaa gaagtataac attgtcaaga ccgtagaaac 126060
tgccgtgttt tcaatcataa ctcccaagaa gtaaccttta tcttgacttt tgtgagtcat 126120
ttccttactt ttattagtta atgtatcatc tattaagtat gtctttaaac agtgtgttgt 126180
cttgtttagc cgtattttga actttatctg ttgaacttat actgtttgtg ttcttctgag 126240
atttacatct ttttctcagc attacgcttt gagatttatt catgttaatg ggtgtaaatt 126300
catagaattt gctacaattt attcatttta ttattaatag agttttgaat ttttcccagt 126360
gttttgcttt catggacatt cctgctatga ctattttaaa catgttttcc ggtgcacatg 126420
tgtaagaatt attctagaat atgaacctgg gagttgagtt actgggtaac atatctgtgt 126480
acattcaaac tttattaggt aacgccaatt tcttttcacg aatggatgta gtagtgtgca 126540
ttctcaccag taatggatgt gtgcatttgt tgttttccat ttttgtcagc acttgatatg 126600
gccagactta agtttttgca agtctgttgg tgatgatggt atttcgtttt ggttttaatt 126660
tacatttcct ttttttcttt tcttgagaca ggctctcacc ctgtcgccca ggctgaagtg 126720
cagtggcagg atctcggctc actgcaacct ctgcctctcg tgttcaaagg attctcctgc 126780
ctcagcctcc cgggtacctg ggatcacagg cgggcgccac cacacccggc taattttttt 126840
ttttttttga gagggagtct cactctgtca ccagcctgga gtgcagtggg gcaatcttgt 126900
ctcgctacaa cctccaactc ccgggttcaa gtgattctcc tgcctcagcc tctcgagtag 126960
gtgggattac aggcgtgcac caccacaccc agctaatttt tgtttttagt agagatggag 127020
tttcaccatg ttggccagga tgatctcaat ctcctgacct cgtgatccgc ctgcctcggc 127080
ctcccaaagt gctgggatta cagacgtgag ccactgcgcc tagtcaattt tttatatttt 127140
tagtagagat gcggtttcac cgtgttggcc aggctggtct tgaacttctg acttcaagtg 127200
atccacccac cttggcctcc caaagtgttg ggattacagg catgagccac cgcgcctagt 127260
cagttttttg tatttttagt agagatgtgg tttcactgtg ttggccaggc tgatctcgaa 127320
cttctgacct caagtgatcc acctgcctcg gcctcccaaa gtactgggat tacaggcgtg 127380
agccactgtg cccggccttt gttgcatttc tgtgatcatt aatgagtttg ggctttttaa 127440
aatagatcta ttggctattt ggattttact tttttggtaa aatggcttct caggtcattt 127500
gtctattttt ctggtttttt tttttcattt taatcattta tttgtggcca tatattatgt 127560
gtcttacata tcctaatcct tagatattat acacgttgca aatatcttct cccaattgtg 127620
gcttatcttt tcattttctt ttgtggtatc ttttgatgaa gataaattct ggtttaaagt 127680
ttattaatcc gttctttttg gtctgtgttc ttattgttta agaagtcttt tcttactctg 127740
aagctattaa gatattttcc tatattttct tctaaaagtt tcatggtttt accttttaca 127800
tttaggtcta taattcatct agagttgact gttattattt tttattatgg tatcagaagg 127860
ggtccaattt tatgtctttc tctgtagata accaattgtt tcagtatcat ttatttattt 127920
aagttctatt ctttcttctt ttatctgtgc attgcccacc ctctcatatg ttgagtgtcc 127980
atagatgttt gaatctgttc tgggtcctct atttcattcc tttgatctat ttctttattc 128040
tggttcttgt tctacatagt cataagtagt acagagttac attaaggcta cctatttgat 128100
agtgctagta cctttttgtt cttctttaag agtgtatctg tttttaagtt gaacaaggaa 128160
tactgttgtg tgtttgtgat tacatttaat ttttagatga ttttggggag aattgaatct 128220
tcacaatttt gagttatcca actcttctat cctgactttt taaggattga ttcttttctt 128280
tctatagctc ttgtgcatct tttagacttt tttctcccaa tgtagatatt gaattattcc 128340
agtgcgttaa gaaaataaac ttattctttc ttcttttgcc tgcacagtat gtactttata 128400
tttttatact actttaaatg gtaaacttta aaaatttttt gttttctgtt tttactgcct 128460
tggtcaggga cttagttgta gtagcaatgt gaaggatggt tgtgtgtaag gaagagtata 128520
gatcaggagt ccagtgagga cagagtggtt gtagtccagg tacagaggat ctcatctaaa 128580
gtggaggtgt tcagaattgg aaggaataca ttgacttgag atggaagaat tctttacatc 128640
aggggtctcc aacccctggg ctgtagacgt gtactcgtct gtggcctgtt aggaactggc 128700
tgcacagcag gaagtgagtg gtaggcgagc aagcattact gcctgaggtc tgcctgctat 128760
tagatcagca gcagcattag attcttacag gagcacacat cctattgtga actgtacatg 128820
tgagggatct aggttgtgcg ctccttataa gaatctaaac taatgcttgg tgacctgagg 128880
tggaacagtt tcaccccaac accacacctc ccacccccgg tctgtggaaa aattatcttc 128940
catgaagccg gttcttggtg ccaaaaatgt tggggggacc gctgctttac acattccacg 129000
ttgtccaaga tgactctaag gtctctggct agaatgaagt gaggaattct aggaagggag 129060
ttattaagag atgacatgtt ttggttttga atgtgctgta tttgaagtta cagataaata 129120
ttttaataag ataatgaata tacttgagct ttattgagtg tcttctatgt gacgggcagt 129180
gttctaagtg ctttagatga attagatatt atctagctta attcttgcag cctatcaatg 129240
agttgtagat tccatttgta ttcctgtttt ataggtgaag cagcatctgt tttacagatg 129300
aaggtctcta aggctgctgt tcagttcata aatggtagaa gtgggatttt aaccgaggca 129360
gtctgactag agtctgtgca tttaaccatt ttattactgt gcttcctaga taaagatgtc 129420
aagtaagtgg ttgcagaatt gaggcagata tcaaagaggt caacactaga aacacaaatt 129480
taatattgtt tgcatagagt tgatagttga atgtgatgtc atccttaatt caaaatggtg 129540
gaaggaacag aggagttctt accggagcga tggcaagagg caagggaagg gaggatggaa 129600
gaggggagtt agggtcaagt ttaggagcag aagaaggacg aattaaacta aggcagagaa 129660
tggtattcac cagatgctgt ccgttttcag agaatgcttt ttttttttaa tgagaagcct 129720
gtgattgtct caaattatgt atttttctct gtagaaaggt aaacaagata caaagatact 129780
ttactgctac tgataccccc tatgtactga acctagagaa tatagtttct gtgattatca 129840
actttataat gtaattaatg gacatagttc tagagtattt ctaaaagttt aaaaactcaa 129900
atataacttg tgtcaatggg ttttgttata tatgtgttat taatatggtt caaagtaatg 129960
aaatgctgtt tgaatgatta agtagaaatg gcaagaaaag tttgccattt aaacactaca 130020
aagctagtgg tagcaagcac aggttccttt aatgtactat tctgcttgat tgaaagaaaa 130080
tttaaaaaga tattatatat gattaagtat ttttaatggc cttgaagact tttgatcatc 130140
acaactaatt cttattaaga taacacttaa actaacattt gatttctttc cagaataaat 130200
acaatcctgt aaaaggtaca ataacagtta acattgggaa aatatcagat tgaggtgaca 130260
tttatacata taaaatcttt cagtgatgga ttactcttgt caagcatttt gctttattca 130320
gatatttttc cagttgtcac cattgcctaa cacaaaacag caaagatgag tagtgtaaca 130380
ttgttaaatg ttaagtcgtg ctacacatgt gccttttctc atcctccagc accatccttt 130440
ccctttgctg tcctctcttg catgcgtttt ggttttccat gagagtgagg taaagcttag 130500
attcaaagag agcctgataa gatgttgctt ctccttctgt tgcacccaat cctttatacc 130560
caaatgtaaa gatttacagc agtggggtgg gacctgtctt tttctaacct ggactcttta 130620
cccatgattg cttcactttg ctacttattc ctgtgtgtca tcatctctcg atcatctgct 130680
tttgttttcc ttccagtact tcttggtgtt aaatagttgt tttaaattat gcatcacatc 130740
attttgtttc tcttgagcca ccacacaaaa gatcaccttg ttttgacagg aataaagaaa 130800
gatgactcta agggatttac tgtctttcat gtgattaata cagtctcaat gattaagatc 130860
cagaaattgt gcatttgtga catgacagtt atggactgaa ttattttgtc gtttgttttg 130920
gggcttaata cttttttcaa ggttttacag tgtttgtatt attgatattc agactgataa 130980
aagagtaaga attttaagta gcaaaaatag agataagagt gactttttgg ttgccttttc 131040
accttagaaa tctgaaatgt tattgccctt actaatttag aaagaatcgc taggaagccc 131100
cctgtgttat gatggcagcc tgctggatgc atactagttc ctcttgagca tttgtcagtt 131160
gccctggctt tgttttatcc attagaccat tttgatttag acaatatggc ctttaacttg 131220
ggggggttct tgagtaaata aaaaagatca aaagtttcaa aaaataaaat ttgctcttaa 131280
tgatagtatc aaatcaaaaa actcctttgg ttaatgcttt atagaagtgt gggatctttt 131340
ccatgccacc tacaggaggt cattcagagg ctggtgaaaa taaacacatc ttgtgtgtcc 131400
tatataaatg acagaaagaa tagccagctt gctttgatca tggactgtca acctctattt 131460
gaccttgtgc attattaacc caaatgggca ctgtggttta gccatactga tagtggatag 131520
acaggagtca cttgttttta actgcctttg cctgtagaaa taaatggtaa tgtcgaaggg 131580
atggcttagg tatctgaatg taattacttg aaacgacaat tgaaaataaa ggcagaactg 131640
gataaagaca aaaaatgtgt gtttgagaca gaggatatct gctttgtata acatcttttc 131700
actataggaa agcaccttcc atgctattct gttgagcaaa acataaatta ttggattgtt 131760
attcctatta tgaaaatatt gtttatgtag atttattttt gagttctttt tcttcataaa 131820
ttgaagttgg gggaggtacc agaatattat ttcaacttga acttgtaaag taaattgctt 131880
tcaaaaaagc tgacttcata agaatagaga agtggttacc ttttcagtta gtaattatcc 131940
agtttaaaag aattaaaaaa aaccctttcc agttctcttg ttaaccacat tcactagtaa 132000
ttttcttttt taactaaact tctaaaagtt acttttattt actttttgag accgggtctc 132060
gttctgttgc tcaggccgga gtgcagtagc acgatcacgg cttactgtag cctcatcctc 132120
ctgggccaaa gcaattctac catctcagcc tcatgagtag ctgggactac aggcatacac 132180
caccacaccc agccaatctg ttattattat tattattttt ttttagtaga gagggggtct 132240
tgctatgttg ttcaggctgg tcttgaactc ctgacctcaa tcaagtgatc ctcctgcctt 132300
ggcctcccaa agtgttgggg attacaggca tgagccacct tgcctgactt ccaaaattaa 132360
tttttaatta aaaaaatttt gtcattagaa ggtaaaaaat cacagggttt tgatttggcc 132420
ttttgtgaga aataagttag atgatgcaag ttatttgagt tatactttaa ataatactat 132480
agtttgttga ttttttgtta aacttagata ttttaatttt aactcttcta attatatgaa 132540
atggctatag tttagtatca gtttcatctt tttgtttaaa atgttagtgg cagattcagt 132600
gaatcattgt gtgcttttac cagtcacaat ctcctttttt cagctatcct tgcccaccaa 132660
aaagtatcct tttacatgga aagttagttc aaaatattag actgaattaa ttagtattat 132720
tggttgggta ttgtctaata agattgtctc ccaacagtgc tatgcttaat atgaatttac 132780
attttcctat catttgtcat ctccttccac tgtttggata ttgtctgtat gctgatttct 132840
cccaaatctt cgtctttgat ctgtatatct ccttacaggc tccagccttg tatatcccac 132900
tgcttccttg acttcgccat ttggatgacc ttgaccatgg acacctcaaa tcatcatgtc 132960
agggcctcct ccttatctac ctcacctttt ctctagctag catttccatt agtattgccc 133020
atcttctttg attgtggtca tcaggaaatt gcattatcat ttcttagtgt aacaagggtt 133080
tttaaaaaaa atttgttcaa tgcctctctc tcctacctct tttcttgcca ctgggctata 133140
gaattttact tcctccctga cattagaaag tttcccaagc acataatgta tttgcatatg 133200
ttcttccctt ccttggactg tcttctctct ttcctccaaa tgcatgccct cacctccacc 133260
caccccacat taatcccacc tggctaaata gttgatctct ggaactcatg tcagagataa 133320
cacctgtggg gtcatcgccc ttctaagctt cacctcacct cttagctcca aggtctgttc 133380
catgctagtt ctttatccca tattaacaat taacatctgt tgttgtgtag tatattctat 133440
tatttgttta catatttggc ttgctactag attgtgagtt ttgaggttag gatactgacc 133500
tcagaaaaat tctttttatg aagcctttga cgctctgact ctactgtgta atgattttcc 133560
ttgtcctggg aaacagatat cattaataga caaagaccat ttgtcaacgg ttgtaagaat 133620
gttctcccaa ggttgtagtg agtcccttga cagatacagg ctgtgtattt gtagaaaggg 133680
aaaaggacat tctaacttcc taatttctaa cttctaatat tgtattcctg agggaagttg 133740
taaaaggagt atctcccttc ttggagtagt cacctggtca tgctggcagg atcttgtgct 133800
cctcactatc ctaaatgagc tccgggccag cttcagggct gtggccatta gattagttaa 133860
agcatcatgt cttgagtgag agagtaggaa aggtggataa aaacctggtt agtgtttggc 133920
cttgagtttg gcacacaatt ataattactc aatacatgtt tgtataatta ggttgaactg 133980
gtttttcctc tttttgggaa aagggtttag tatcgttatg ctgtcattgc atcaagtcat 134040
cgttaatata tttatgttaa gtacattgta agttgtataa gtaatataat tgcattaata 134100
tcagaacact tgtcttacat ttgagtttca aattgtccag atttgagagt gagcaaaata 134160
caccagatat accaccaaaa ttgaaaaaaa aatcaactgc ttgctgttgg ggaagaagta 134220
gtaatgttgg aaaggttgac ttgatagagg attttgtaag atgagtgaaa aagatctaaa 134280
aggacagtga tgtctctgtt attgactgag gtatccttgg tctctagaat agtgcctgat 134340
gaacagcatt gagttactta ttcactgaat tgaattgaaa gatttgatga aggccaaatt 134400
tctctaaata cattcttgtt tatggattag gtcttattat ctatcatatg tgaatgaatt 134460
cattttattc atcttatgtc acagttttgt ttagtctatt ttctgttgtt gcgaggattt 134520
tgtaattttt tcctcatgag tttgtttgga gttgataatg gtatttgagg agcaagtttt 134580
ctagaaacat agatgaagag ttttttgtaa agtagggggt gagagttatg tatgttcaga 134640
gcagcttctg accatttcct attggaggct tcaagttgtc ttctaactct ggaaaaacat 134700
ggagatgagt gagaggaaag acagaatatt atcatgtgat gtatgccagt gagcagtttt 134760
attggaaata caaaagaaag tctattttag tgtttaattc ctggtttaca taaaaaaaaa 134820
ccaaatgaaa ctgtaaactt acagttaaaa gattagtaag ttaatttgca aatcagcaac 134880
agaaaaagca gttcagaggt ggtaggccat tttaaaatgc aaaactgagt tttgtagata 134940
tccaaaatct ttttgaagac tagtattttg catattttat gattaatgtt acatcaacag 135000
ttgctttgtt tttgccattt taagcaagag agtgagtgaa agagggcagt gtattatgtg 135060
ctggcaacag ctggctgata gcattgaaat acttacataa ttaaatatat gtgttggctg 135120
ttgtcctaac aactctgtag gtggaggctc cagaaagcat gcagaaactt gctttagcag 135180
gattaaagaa aaaatcaaca tgatctaagt ctgttctttc agccaagagt gatttcctat 135240
tgttttggtt tgaaagacat tctacttatt cttggtgtgt gtgtttatat aacagcagag 135300
aaaaaaaggc tcttgttttt ttcttccctt attgtttgct ctaagagtga caaacataat 135360
ctttcataat ttttagtcag tagtattgtt taataactga taataagtga aataaaaaag 135420
actttttaaa cccataggac atgttaccaa agtattaaat attaaccctt tttatttctt 135480
acttatggtt acacttaggt gtcgttctct gggccacatt aatgaagtta tttagattgt 135540
aggctatatt aaggcataga ttgctatgat cctgggaaat tggataggag tggcaggtaa 135600
tatgcaactt acttttacct aacagctgtg gatctaaatc ttactcaaag tgccctatgt 135660
ttacatattt tactgttttt gtagctctaa ggctgaggct ggtccctact gtacctttga 135720
tgaaaggatg gcacttttgt gggtgttaga gtgcatagac acagtgcagc accactgtgt 135780
gtggctgccc catattaata cctgtcattt ctttatctgt ctgcacccat tttcagagtt 135840
ccttaggggc agccactata tcttttatct gtatcccagc atcttataca gtgattgaca 135900
gtgatagttg aacaaatgaa tgaaccaact catatgtatg tttcctttgc atgtttatct 135960
ttcccatccc ttccaccttc tgtgtctgtc agatcaaatg tcttccactg gattttcata 136020
gaactttttc tgtgtctctt tcttattaga ttttaagagt ttgaacttga ggttatctgc 136080
atgtcataga aaacctaaaa agtagaggct tacatgactt aggggattta tttttcgtac 136140
atattgcaaa gtccacagtt aggaaattgc tggctttgct ttaccagttt aagggtgtga 136200
ggattgaagt cggttgtttt aaaggtcttt cccttttggt gtcaaatggt tattgctctc 136260
tggccatcac atgcactttc ccccacaaaa aagatggaag taccaaactg gatgtgccgg 136320
ctgagtctgt cccttcttta aaggagcttt ataggaagtc ccgctcaaaa cccgtgcttg 136380
catctcattg tgtggagtca tatcacagga ctacctctat ttgtaagaga ggctgggaaa 136440
tgtagtgttt ttttttcttt tgtttttata ctgcacacat tgttgctccc aacagaattg 136500
ggattctgtt agtaagggag atggggctaa tgtttattgg cttgatatct ctgctatagg 136560
gtataaactt tgggacaaga tacacatttt atttatcttt ctatcattta tcaggtccat 136620
tgtattggag gaggtcctca acatacttgt gaacaaatga tcctcttttc tcctcttatc 136680
tttattcaga acatttaaga aatgacatag ttgacatgta agaacttttc ctctttttgc 136740
ttactctgag tggtctctga tgaaggaata acagaataca taggagaagt tcatggctta 136800
gttgttggac acacgtattt tttaaaaaat gggacttaca tatattttct gctcttaaga 136860
cctgcaatga ccatatttga aatggaatct ataaggaaaa tatgtttttc attgttctga 136920
gattcatttc tgtgatataa taatcataag cctgatttaa aacctagata ttttaaaata 136980
atttagacat aaaacaaaag tggagtttat gatgattatc atggagggtc ttaaccaatt 137040
ggaaaacagg ttgaaggaca gttaaactca ttttataatt aagcgtttag aaaataatat 137100
tttgtttaat tctaaaaaaa tttaggtaca gctgaaaaat accacctctc actaagatgg 137160
aaatttaatg ttttagattt cacttgaaaa aaaaatggag aatttgatat aaccagtgaa 137220
aatctaacca atgggaagag tcttacaaaa cttgctggag attttttata ggttggcgcc 137280
attggatatt atcagaggga tattaacgct atcaaattct cattccagga tacaacctac 137340
acattctgca gcagtgtctt ttttgtgttt ctgtacacag aaactgtcga gaactaccca 137400
gatataatta ggttctcctg acccattctt tactgtttct ttgtgtgcat gtgtgctcgc 137460
acgctcaatg tagatgcatt aagaaagaaa ctgacagaat cacatgatag aaagtgtttt 137520
gggaaaaata caaattaaaa tggatgtcaa ggatctcttt aacaatatgt tttagaagtc 137580
aacagctcat cctttgtgcc aaccaacaat ttgctttcca gctcataaat ttcgtttcca 137640
ttacaagagg aatcagataa gcaagtttat ggcaaaacta aaaagtttaa tttggtgctt 137700
tccttaagta cctgtcagta ttttatgata gttactgaag gaaaagactt gttttacctc 137760
aaatgagaaa atggtttgag tcaacatatt ttgtagtatt tcagtcatcc atggttgaaa 137820
cattgattaa ctcttttgat gcttttacca ttttaatttg ataacaaagg atttgatgtc 137880
attgatcaag taaatttttt aaaaggtaat ttttaaaaat agggaataga actggagtgt 137940
attcattgag caaattctaa gaaactttaa aaaattctct tctatctagg ctgcaagctt 138000
tttcttactt gtcatttcag atacttgtat atcgagaaat ttgcaatata ccaacttggt 138060
aatcggtaat gggtggcttg aggggtatac agcataatta gaacttaaaa ctcagagtaa 138120
tccagggcag atggtggcct gagtatgtat tttcgatccc ctcattgtca cactgcctaa 138180
ataactttga acaaccgtgg gagcctttgt acctggtgtt agaggggagc acatatgcag 138240
ctgagactct gggtcttggg cagaagttgt gctgtataga tggggttggg ggagtgatct 138300
tatctgtatt ccttgtggtg atggagagaa gagtccagga cctagaatct ggagagtctt 138360
agctctccca tagacctggg gcagtgtctt gaaaacttat cagacccagt aatcctctta 138420
tataaaaagt gttttgcaac gtacccttta ttatactgaa gtgaaaatct tgaataatat 138480
aatctatgca cacacataat taacaaatca attagcacag tgctctgaat gtgattaata 138540
taaggaaaaa aagaaaaaat tgttaatgca atacgtattt caaaatacaa atgcttaggc 138600
acaaatatac tagaagacct aatcaaatgg cctgtataca gaattacagt gacagtgaga 138660
gtaatgaatg cagtctgata tgtgtgtagc cttaacagct taaatgccaa gaatagcatt 138720
ggtgatctag tgatgcacct ttccagaatg gatgatgact cttgctaaag ttcttaatga 138780
cacaaagtat gttcttcctt ccattttcac agaggttgga ttcctgaaac tgtacaagaa 138840
atacttaaaa taagtcctca cttaatgtca atgataagtt cttggaaact ataattgaaa 138900
cgatgtatag agagatgagt tttttttttt ctaagcaatg ttataaagaa actgtcgaat 138960
gaaacaattt tattccagga gctactatat gtaaaatgga gataggttct aaggagatat 139020
tataaatata ttttttacgt atatgcatgt taagtggggc tttcagaact catgcaggtc 139080
gtgtggaatt ctctgtacag catagatctg tgtagcggaa gacatctagc acctctgact 139140
tcggcctgct taatggcagt tgcacccccc caattagtat tatatccaaa aagatttgga 139200
tttaaccact tactgattaa aatttctata tggcctgaag cacacaaaac aagataaaag 139260
aaaaacagga gaatatggat gtgaaggtat atatgacatt atgtttataa gtttaaataa 139320
atactattaa agagctcctt acattaggca acataattag aaaatatcca aagggcataa 139380
acaggcaatt tatgaaagag gatgtgcaaa taaaaatgga aagattttca ttcttaccag 139440
tgctcaggga aatacagacc aagtgagata ttggtttttg cccactagat tttttttttt 139500
tcccagtagg cccagtgctc ctgagagtat ggggaactgt tgtttactct gatacatttc 139560
tggtagaagt ttgaattgct ataatcttct gtggaagttg ttttttgata ggtataaaat 139620
ttaaaaatat atctgcccct tgatttagca gtcctgtgtt ttatgaccct ctcctacaga 139680
catgaaatac ctgtttgcta ggataggaac ataaagttat ttattgcatg attatttata 139740
atgcaaaaag tgttataaca aatcttatgc cgtcttaggt attcttcttt atgggcacat 139800
attaatacat tttacagcag ctgatcctgg aatctactta catagatttg cccggctgag 139860
aagcaacttg agggctgatc gcccgctgca ttccaaagaa tccttttttt tttttttttt 139920
gacttattag tgattatgat gacattacct tttattgaac tgtttcaaaa tacatgaaac 139980
catgctctga tgacattttt gaagtgtctg gattctgttt gccactgttt aaagatttaa 140040
ctagttcttt ctttaattaa tatttagctc ttctagtatc atcttaccac tggaacagca 140100
tgtaccattt gcctcatctt tccatttcag ggctggtcaa aagagacaaa tttcagacat 140160
taactaactg aacattcttt actataatag atttggaact tttatctaaa acaaatgtct 140220
atgttctctg gccactgtgt taaatatgta tggtgtgtct cttccatttg gcagcaaata 140280
tccattgcat ttgattcagt tttcactgcc cttgaaataa atatacttgt cctgaatggc 140340
aggcttaagg attttccaaa gtaaatgatg tttgcaccac atctggccag ccatttttat 140400
gtgatacatt aaagagagtc agtgttcatt aatttgacaa atatttcttg aatgcctact 140460
atgtgttaca ggctttggca attcaaagat taatgacaca gtcactgctc ttaggagatg 140520
acagtttagc aaaaagggca gagtcttagc cagataatta taatacagtg agggaagtgt 140580
ggtaatgctt ctttgttgac aatgatacct gaatgggtgc gaatgtacct cagtttttaa 140640
aatcctgggt ctttttcaag tctatactca tcctgggtct ctgtatctta ttttacaccc 140700
cccttccctc aacaataagc aaactttgct atctgaggta acaagaaatc ttgaggaagg 140760
gcagctccga ctttattaat tcagttgttc aaagatacta tcaaagaccc taggcactaa 140820
gtacttcgta ttttttcctt attttttctc ttctgccacg ttcaatgtga tggcttggtc 140880
ttcagtgaag ttaaaaagtt actttcataa ggtggctgcc tgggttttgg ggaccatgtg 140940
tagaggaaca atttctttcc tgtgtgtact tttctggggt aaattttaaa tcattttaga 141000
ctaatagcaa aattgtaaaa atagtacact gaatttttgt atactcatta cctagattta 141060
acaaatgcta atattttacc atattatttt acttttttgg tgtatgtgat tttcatggct 141120
aaaattttaa aacaaaaggg tgtgcaatgg aaacttcatt acagctctga tgttagccac 141180
cttatgccct ttaccataat gtatacaaat gactatgtca atacctttcc tccctttctc 141240
ctttgtctct ctttcgcttc ttctctctcc tccctttgtg tttttccctc cccctctctt 141300
tcttccctcc ctgcctccct tccttcctcc aattgtgtat cctggaataat tccatatgg 141360
atatatacaa aggttcctca ttctttatag attcatagaa tatttgtttg ttctcttatt 141420
tatggatttt tagtgttcca gtttatgtgc atgaaatttt attaaccagt cacctctcga 141480
cttttttggt aataattggc attataaata ctgcttagaa gaatgttttt ttaagtattt 141540
atttatattt ttagagatgg agtctctgtc tgttgcccaa gctggagtgg agtggctatt 141600
tacaggtgca gccatagcat attgcagcct tgaaatcctg ggctaaagcc atctttctgc 141660
ctcagcctcc tgagtagctg ggactacagg catgtgctac tgcgccagct tataaacatg 141720
ttttgactgg aaaccttagt tgttgactat gtagagtatg taaacagttt ttaacttgtg 141780
tcttgaaata ataacataat tactgaaaag ttacaaaaat agtgcaaaga cttcccatgg 141840
acctttcacc cagcttcctt taatgtttaa atattttaca tagccatgat gcagtgatca 141900
aaccagagaa ttaatattga tacagactta attcacattt tgtctttttt ccagttcagg 141960
atccagttta ggattctcca ttgcatttag ttgtcattca ctgtcacctc caatctggaa 142020
cagatcctca tttttttctt cttcttttat gacattgacg ctgttgaaga atacttgtca 142080
gttgtgttat agaacattgg tcagtttgca tttgcttgat atttcttcat ggttagattc 142140
agatgctatg cattttatca agaatatagg agtggtcgat attgcacttg agattgacaa 142200
atatcagtgt gtcataccaa gaagattcag gctgttgata tatctctgta ccagttaact 142260
ttaacatcta gtaaccaggt gtaaacttta tttaaggtgg tagctgtcag ttttctccac 142320
tgtaaagtta ctgtcttctt ctttggaatt aatagatatt ttgggattct ttggtactgt 142380
gcacatatcc tatttctcat catgctctca ccattataat tttagcatcc cttggttatt 142440
cttacctgta acaatcaata caattgtatt atcaagtggt gatttttctc tttccatcat 142500
gtcttctcca cttattaatt tgaatttttg agtgtaaaga agagctacag aatgagtctc 142560
ttttctctca tttatttatg tattcaatta tttatatcag tgtagcctca tggttcttta 142620
ttttttgaga cataatccgt aactatcatt atcacccatg ttgttctgga cttggccaat 142680
ggaaatgtct tcaggttgtc tcctttatct ttttgttgtt gtcgtttgtt ttcttattga 142740
gtgaggattt taatgacaag ctatggaact cagcttatac taatgagaat ggtacaaggc 142800
ttgactttcc ctccgcgcat cttaaccatt tttaaatgca gagttcagca gtattaactc 142860
cattcacact gttacccagt gctcctgtat ccttttaata tgcttctgtt ttgagcactt 142920
ccttatttcc cggcaccata agttcaggct tatcgtgtac tttccttgcc tcaagtcctg 142980
gtttgagtca tttcttgaag aagccctggt tcctttcact ggtgaactgt gtgaagaaac 143040
cgcaatctgg gcactagata tgctcattgc tactaggatg tcattgcttc caggcactct 143100
tagtagactt aaatctacta agacttagga aatgtatgta tttacacaaa cacctataac 143160
tatttctact tatctgtata tgtattaaaa aaacacgagg ttgtactctt ccctttgatt 143220
ttacttcaac tggataggat ttgattttag catctccctt ttcttataag taactctgct 143280
ctttaacagt gaaaaacaga aagtctcata gtctacaata tatttacatt atttgttcaa 143340
tcctattaat catatacagt ggtttcagaa ttactaaccc atctctgtga aaaagaaatt 143400
ttactaacac aaatataata tttatgttta gtcctttttg tgaagttcac ttgggttagt 143460
tctttccttt gccactcaaa tcaaattctg tttttaattt gtaatacaat tagattaatt 143520
tgttaccact tgtattccat tgtggattct ccccacttcc tggatggttt taattttttg 143580
agtttgtgaa atatgagcat ggccctcaaa gtcagaatga tattaaatct tttactcaga 143640
gaatgatcac tgcccactcg tcctttttgc catccccatt actccattct ttccatcttg 143700
agcccacccg tctcccatta gcttttgact tctccttcca tttatttatt tattttttgt 143760
acaaacaggc agaagtcagc agtgattttt cgtattttga tcatgccttc tacatttgtt 143820
aattggaatt tcactgtaag gaagagtggt ctcttattcc ccatttattc aattatttat 143880
gtcagtgtag actctgatta tttattctgt aggtcataat gtcatagaat aaatatacag 143940
aattctctgt atgaaaccca tggaaatttt taaatttcct tttgtttttt acttaaatga 144000
tagcatacta tagatactat tttacttttt gtaatgtaac ctgggaactc tgcatcagtt 144060
gatagaaatc ttcgtcattc tttcttatgg ctgcttagtt ttccatgcag tgagtgtata 144120
gtggtttatt caactacttt cccatgtgtg ggtgttgagg ttgtctgata tttcacaatt 144180
ccaaactgtg tggcagtgag tgaacttttg cttattgatt tttatattat tggaggtata 144240
tctttgggat gaatttctag agtgcagact gctggattga aaggtaggta cgattataga 144300
tactgcctag ttaccctcca aaagggttgt aacactttat atccttacca ccaagtgtga 144360
aaattgtgtg ttccttgcaa cccctcaaat ggaattttat tgttgtcatt ctttataatt 144420
ttcaccattc agtagggaag aaatggtatt tcagggtttt catttttcat ttctgtaatt 144480
gtgagtgggt ttgagcatct tttatagttt ggccatttag atatactttt gtcaatcatt 144540
catgtctctt attgtttatc tattgtctct caatttgtgt gagtttttct gtttgtctgt 144600
ggcatatgtc acaggcatat tctccccttt tgtctgctgt ccttttactt tgttgtgcag 144660
aatattttat tctttgccat taaatttatg aatctttttc aagttctttt ggattttgag 144720
tcatggctaa gttatcacct ttgtgtaaaa agaggctaaa gagacattta cttttttcta 144780
gtacttgtca cttacatagt gacatttata tctccaatcc ttttggagtt tattcttatc 144840
tacggtgtga agtatgaatc tacttttttc tttgtgtaac ctaattgttc tagagctctt 144900
tttatctcta gaaatgttct agtgctcttt tatttttttt aaatctctaa atttttttta 144960
gtgatggggt ctcgttatat tgctcaagct ggattgcagt ggctgttcac aggtgcaata 145020
atggcttact acagccttga gatcatggac caacatgatt ctcctgcctc tgcctcccta 145080
gtagctggga ctctaggcac acaccaccac acctggtgct tacactagca ctatttacta 145140
aaaatttagt ctttaccacc agtgttttgg gatgccattt ttgtcaaaac gaatttccaa 145200
atttacgtag gtctattttg ggctttctgt tctgctccac ggttctgttt atttgtattc 145260
catctacgct gtttgcaaag gaagttttat ttatattata gtttgacatc tgctagggct 145320
agtcctttct tgtagtctgt ctcccttccc ttccctctcc ctctccctct ccttctgcct 145380
ctccctctct ttctctttct ctctctctct ttctttcctt cctttctttc ttcctttctt 145440
tcttttcttt ctctttcttt ctttctttct ttctttcttt ttctttttct ttctttctct 145500
ctctctctct ctccctctcc ctctccctct ccgtctcttt ctctctctgt ctctctctct 145560
ctctttcctt tctttctttt ctttcttttt tgagaatctc actctgtcac ccaggctgta 145620
gtgcagtggt gcaatcttga ctcactgcaa cctccacctc ccagattcaa gcgattcatg 145680
tgcctcagcc tcccgagtag ctgggattac tattagccac catgcctggc taatttctgt 145740
atttttaata gagacaggat ttcaccatat tggccaggtg gctggtctcg aactcctggc 145800
ctcaagtgat ctgcccacct gggcctcccg cagtgctggg attacaggtg tgagctaccg 145860
tgtctggcct agtttttctt tttaaagtgt ttttctgagt attctttcat gtgaattttt 145920
tcaaattaac tttagtatca tctgtgtaac tccatgaaaa attgacttgg tatttgtatt 145980
ggtaaattag aaattaattt ttttcttttg agacagagtt tcactctgtt gcccaggctg 146040
gactgcaatg gcacgatctt ggctcactgc agcctctgcc ccccgggttc aagtgattct 146100
cctgcctcag cctcccgagt agctgggatt acaggcgcga gctaccatgc ccagctaatt 146160
tttgtgtttt taatggagat ggggtttcac catgttggcc aggctggtct tgaactcctg 146220
acctcaagtg atccacccgc cttggcctcc cagagtgctg ggattacagg cctgagccac 146280
agcgcctggc tggaaattaa tttttaataa attaatttag ggagaactga tagttttagg 146340
aggtgaagtc cttctgttta agaatagatg atggcttcac atttattcag ctctacttct 146400
gtgtctttta gcagtgtctt catgttttcc cagtgtaggc tttggatata cagatgtcat 146460
tccagatctg tgggggattg gtccaggatt ccccaaagat accaagatct gccattgctc 146520
aagtgcctga tattaaatgg gcgtagtact tgcacataac ctatgcatat tctcctgtat 146580
actttaaatc atctctggat tacttgtaat ccctaatagt ataaatgcta gataatactt 146640
gttttagtgt attgcttagg gaataatgaa aatgaaaaga tctgtacatg tttagtacgg 146700
acacagttgt tttcccctga gtatttttga tccatggttg gttgaatcca tggatacaga 146760
acacatgtga tatggtctgg ctctgtgtcc ccacccaaat ctgatcttgt aactcccata 146820
attcccatgt gttgtgggag gaacccagtg ggagatgatt gagtcatggg ggtgggtctt 146880
taccctgctg ttctcatgat agtgcatgag tctcatgaga tctgatggtt ttaaaaaaca 146940
ggagttgccc tgaacaagct ctcttttttc ctgccgtcat ccatttaaga tgtgacttac 147000
tcctccttac cgtccgccat gactgtgagg tctccccagc cacatggaac tgtaagtcca 147060
ataaacctct ttcttttgta aattgcccag tcttgggtat gtctttatca gcagtgtgaa 147120
aacagactaa tacacgatgg atacagatac cttgttgtag ttattccttt atgttttatg 147180
ttctttgttg cctttgtaaa tatttttttt tctgcctttc atagaggatt tctctctcat 147240
tttgacctct aactggttat tacttgtata tataaaggct attattccta tatgttcatt 147300
ttatattgtc ttactgaact tttttatagt ttgagttaat ttttatcatt catttttttc 147360
tagaattatt tatggtactc tcatttcatt tggaaacaag agttttaatt ctttgctttc 147420
aaattcttat tctgttattt cttttgtcta atagcagttt cttttgtcca gtttgtcttt 147480
tgtctaatta cagtgttgag cagtagtgga gatagtggat aatcttggct tgttccttgt 147540
gttagtggaa ttgactttag tacgtccccc attaagtaag atactggctt ttgcactata 147600
tctattttgt catgttaaga aactatccat acatttctat ttttttagag ttttttcttt 147660
ttaaaaaatg gatacgcagt aaccatacat atttatggag tacatataga tattttagta 147720
catgcataca atgtgtaatg atcaaatcag gataattagt ttattcatca cctcaaacat 147780
ttatcatttc tttgggttgg gaacattaaa aatcctctct tctagctatt ttaaaatata 147840
caatagatta gtgttagcta tagtcaccct actgtgctgt agaacactag aacttattcc 147900
tcctgtctaa ctctgcagcc tctttctatc ttcttttccc ctgctccttc ctagcctctg 147960
gtaaccatga ttctactctt cttccattag atcaacttat ttagctctca cacatgagtg 148020
agaagatgca gcatttgtct ttctgtgcct ggattatttc acttaacata atgttctcca 148080
gactcatcca tgtatgccta attgctctga ttaggacttc ctcttagagt tttatcaggt 148140
gtgagtgggg agttttgttg aaagcatttc agaagattga tggagtgatt atttttcctc 148200
ttacatctat taatatgaca tatagtgtta gtagattttc taatattgaa tttacctcgt 148260
gtttctggag taaatttcat atggtttttt ttttaaattg ttgtattctt ggcaaaaaat 148320
aataaaatat tatcatttgg ggtccatttg tgatatttgt ctgtagttat atttttttga 148380
actgtctttt tctactttgg gcatcagtgt tatacttgct tcatttaaag aattagaaca 148440
tttttattca ttttcagcac tctggaacaa ttcacagata attggaacta tctggttttt 148500
gaagacttag aatttccctg agaacacatc tggtcctttt gcttttttgt gggatggttc 148560
ctatataaat ttctgtatat gttctttaga aattggtctg tttaagcttt ctatctctaa 148620
ttggggcaat tttggtaatc tgtatttctg tatgaaatta cccatatcat cttggtttta 148680
aatatacagt catgcgttgc attttcagtc agtgatggac tgcatatatg atggtggttt 148740
cataagatta taatggagct gaaaaattgt tatcacctgg ttatatcata gcaattgtaa 148800
tgttgtcaca caatgcatta ctgaagtgtt tccggtgttg ctggtgtaaa caaacctact 148860
gtgctgccaa ttgtgtaaaa gtatagcacg tacaatcgtg tacatacata atacttgatg 148920
atgataacaa atgactattt tactggttta tgcacttaat atgctaaact tttattatta 148980
ttttagagtg aactgtttct actgattaaa aaaaaaaagt taactgtaaa acagcctcag 149040
gcaggtactt caggagggat tccagaggaa gtcattgtta tcacaggaaa tcacagctcc 149100
aagtgtatta ttgcccctga aagtcttcta gtggagcaag atgtggaggt ggaagacagg 149160
gatagtgatg atcctgaccc tgttcagacc taggctaatg tgtgtgtttg tgtcttcatt 149220
cttaagaaaa atgttcaaaa ggtaaattaa acgtttttta aataaaaaaa ggttatggaa 149280
taaagataaa ataaaatatt tttgtacacc tgaacaattt gtgttttaag gtaatcgtta 149340
ttatgagtca aaacgttaaa aagaaaaagt ttacaaagta aaaaagttat agtaagctgt 149400
ttagtttatt attgaaatat ataattttta ataattttag tgtagtctaa atgtacagtg 149460
tttattgtac attttagtgt tatgtcctag gccttcacat tcactcaaca ctcacccact 149520
gactcacgca gagcaacttt tagtcctgca aactccgttc ttggtaagtg ccctagacag 149580
gtgtaccatt ttaaaaaagt cggccggcac aatggctcat gcctgtaatc ccaacacttt 149640
gggaggccga tgcgtgcggg tcatgaggtc aggagatcga gaccatcctg gctaacatgg 149700
tgaaacccca tctctactaa aaatacaaaa agttagccag gtgtggtggc aggcgcctgt 149760
ggtcccagct actcaggagg ctgagacagg agaatggtat gaacccggga ggtggagctt 149820
gcagtgaccc aagattgcgc cactgcactc cagcctgggt gacagagcga gactctgtct 149880
taaataaata aataaataaa taaataaata aataaataaa ataaaataaa ataaaaaagt 149940
cttttatgct gtatttttgt atgtttggat atacaatact tactactgtg ttacagttat 150000
ccacagcatc tagtgtagta acatgctgta tgggtttgta gcctaggagc aatagtctgc 150060
aacatataat ggcctaggta tatagtaggt tataccatct aggtttgtgt aagtacactt 150120
gaggaatgat gaaattgcct aatgacgtat ttcttggaaa gtatctccat cattaagtga 150180
cacatgactg tatttgcata gaagtctgca aaagtgacct cttatgactt taaaataatt 150240
tcttctgttt ttattattat ttatgccttg ttttttattt gataattttt tttgtgtgtg 150300
tgtgtgatag agtcttgctc tgttgcccaa gctggagtgc agtggcgcga ttttggctca 150360
ctgcaacctc tgcctcccag gttcaagcga ttctcctgcc tcagcctccc tagtagttgg 150420
gattacagac acctgccacc aggcctggct aatttttgta ttattagtag agatgggctt 150480
tcacctgttg tcccggctgg tcttgaactc ccggcctcag gtgatccacc tgcctcagcc 150540
tctcaaagtg ttgggattac aggcgtgagc catcgtgcct ggctaaattt gttttgtttt 150600
gtttgtttac tttgcttttt tcatttttca acgaaggctt tatttttttt tagatggagt 150660
tttactcttg tcgcccaggc tggagtgcaa tggcacgatc ttgcctcatt gctgcctcca 150720
cctcccaggt tcaagcgatt ctcctgcgtc agcctcccta gtagctggga ttacagtcat 150780
accccacacc agggtaattt ttgtattttt agtagagaca gagtttctcc atgttggcta 150840
gcctggtctc aaactcctga cctcaggtga tctgcccact tcggcctccc aaagtgctgg 150900
gattacaggt gtgagccccc atgcatggcg agagctaagt ctctaattta tcttttattc 150960
ttgcattttt actgctaagt tttagtaata tgaatttttc tctgtttact gttttaaatc 151020
ctagagatgc tgatgcatag tgtgctcaat atcactagtt tttagaaatc ttctaattta 151080
ggtttttatt tctcctttca cccaaaagct gcttaaattt ttttatttct aattttattg 151140
caatgtgatc agagttattt ttaatttttc tgctttaaag agcttcctga tgtcctttat 151200
ttgtgattta tcattttgtg aatgttcaat gggtgcttga aaagaaggtg tattctcttc 151260
tctgttatta ggtagtaaca ctgaatctag attaagatct gtgttgtttt ttttttttga 151320
ctctatttaa gtctttgtac tatcattgct tgagtcttgt atggagaatg tataggtctg 151380
ttattgtgaa tgcatttctt cttgcagcct caggttggtt ttatgttatt tggtgcatag 151440
gtatctatag atgttctaat tttattgtca gttgtgtctc taaagtgttc ttcttgggct 151500
gggcatggaa aaaacaaaag tgcttttctt ttcatattta ttcaattttg gcttggatta 151560
ttctttgtat gttactcctt tgtctctttt taattttagt cattctgaat tatttagctt 151620
taggcatgtt tctggtatgt ggaatagaat tgggtgttgc tttgtgaacc acatttaaaa 151680
tctttttctt ttaataggtg agttaagctt ctttacagtc agtaaggctt cacttaatgt 151740
cctggataga ttcttggaaa cacagtttta agtgaaatga attactatat gttgtaggaa 151800
cttaactctt gctcatatca attaacctat ggtaaattgt ttctttatac tacagttgtt 151860
tcgcttaaac ttgtggtttc caagaaccta ttgatggcat taagtgagga cttactgttt 151920
gatgtgacta cattgtttgg tctctgctct gttgtgatgt tttaaaatta tatatattat 151980
tttttattta ctatgtttct ttgtgacatg tgtttttttc tttaaataaa taaagtgtta 152040
tttaggaagt tgcatatttt gagtggttaa cttttcattt ataacttcta aatgcctgta 152100
gtttcattgc ctatgttttt gtttttgttt ttgagacagg gtcttcctca tacccagtct 152160
ggagtgcagt gtcatgaggc atggttgtag ttcattgtaa cctgaaactc ctgggctcaa 152220
gggatcttcc cacttcagcc tccctagtat ctatgactac gggagtgtgt caccacacat 152280
ggctagattt atttattgat tgattgattg taaagactgg gtctcactat gttgccaagg 152340
ctggcctcaa actcctggcc tcaagcaacc ctcctcctgc cctggcctcc caaagtgttg 152400
ggattatagg catgagccac tgtatccggt ttccattgcc tgtttaaaca tttaccatct 152460
actttgtcag tgttcaatag tatcctttta ttcctgactt ttgccaacca aagaatgtgt 152520
tttactttct gattttctca ccctacacat ttttctgcat tatttctatt tattataaca 152580
tttgtacatt gacctacttt tttcctactt ttgtgcattg ctacaatgca tagcatttgt 152640
acattcatct ttggcttttg acaattaata ttcccccctg tattttagtt ttagatctac 152700
agttatgtag caatgagctc attatcagtc cttttgctga aatttcccca gacctctctt 152760
ggttgcatga agctcatcct gttttattta gattcttcaa gaggggatta tggattcttg 152820
tgtgttaaaa actttttata tagccttgaa acttgaagga taccttggtt gtttttaaaa 152880
ttcattattc atacttttat tgaatttcta aaaaatgctt ttccattgtt gcattatatt 152940
ttgttttaga caactctgat gccagttgaa tttatttgcc ttcgttcatt attttatctt 153000
ttatgcctag tagccttgga cactttttct ttgtttttga agcctaaggg tttattagga 153060
tgtgtcttgg agttgactgt tccaagtaaa ttctcccagg tactggttga cccttttaat 153120
atgcagattc aagttttctt gaattacaat cttaaatatt gtttctattt atttttattt 153180
cttttttaaa atttttttgc tgggactcta gttttatcaa cgttattttt ttcttttttg 153240
ctttctgatt ccatcacttt ctctttgact tttaattttt tttaattttt atttttttct 153300
gagacaagct cttgctctgt tgcccaggtt gaagtgtagt ggcgtaatct tggtttacta 153360
cagcctccac ctcccaggtc aagtgtttct tgtgcctcag cctcctgaat agctaggatt 153420
acaggtgccc accaccatgc ctggctaatt tttgtgtttt tagtagagac ggggttttgc 153480
catgttggcc aggctggtct cgaactcctg gcctcaagtg atctgcatgc ttcagcctcc 153540
caaagtcctg ggattccagg cgtgagccac cacacccggt ctaattttgt tgagttttaa 153600
aataattttt atttagaatt ttatgtaggt gtttttaaac atatgacatt atatttgaaa 153660
tttcttacaa atatttagta aaggaaggaa aactaaagaa gaatttcctg acaataaagc 153720
gctgttagca atgtttgatt ccttacattc ggtgctcaaa ccattactat tctaggactg 153780
gtccttttta aggatggttg aaactaggac agattgtcac ttgtctggaa ttgttctttc 153840
tataagcaag actttattaa tactgaatgt tatctccctc tgtctctgtc tgtgattctc 153900
ttgaaagtgc ttcattctga ctgaaggaaa acttttgctt tctgcatgga gctttgtata 153960
gaaacccggc acgacagggc actttcgggt catgccttga gtcttaagaa atattgaaaa 154020
atataatcta acaatgatgc ttctgttcag caaggcaagg aatttataac agggcaaaac 154080
tgaaacctgt ttcttgtttg ctagcccata aggaggcagc ttgtttttat catcagtttc 154140
ctgacatcag gatgtgactt cgagaagagg aaaataacac ttattggcat caaagatact 154200
aaagtattta atgccttaaa tgaataatca gtatatgtga acaatgaaaa cacagatgag 154260
gaaggatacc cagtttcagt ttcatctgtt gtatcatgtt agttggagat atttagctta 154320
agcctcttcc ctgttggtcc actttaccct agttgtttca ctgttgtgaa tgcatttaca 154380
attattttaa aagatggcct cattctgtag gtgaatccct gtaaagaaca ttgtagaagg 154440
ttattataac aactcctaag cataagctgg agtctaaaat gtttctaggc agcaacagtc 154500
atatgtatgc ttgacctttg ggagatctgg ttctttcctg ttgcttgttt ttaccctggc 154560
actgtcaccc acttgtgccc aggctagttt caaccactgt ttgcctgagc ctacattttg 154620
tgtttttttc cattttagca tgttttacaa atttattgaa ttacactttg taccaggtat 154680
tgtgttaggg ggcaggtata aaagcagagt tctcatctgt tatttgatag gaaagacagt 154740
gtgactgtta tcagtttact tattctcagc ctgcagccca gccttgtttg ccttgccttg 154800
ggtgacataa ctggactctg taatagctgt tctttgtcag ctgctataat gttagtagat 154860
ggtcttggtg gggaattgca aagtggtggc agcaggaaag cacttgtctt ctgggttcca 154920
gttttcctcc atttttaatt cactccagaa gcgagagatg ggtgcatgga aggcctggtg 154980
gagctcatag gatagtatcg atactgcctc ctttcccatg acattacaga ctgtcttagt 155040
ttgctcaggc tgctcttaca aaataccata gagcaggtgg gttataaacg acagaaattt 155100
aattctcaca atactggagg ctgggaattc caagaataag acgccagcag atttgatgtc 155160
tagtgagaac tcacttcatg gttcatagat ggcaagttat agctgtgtcc tcacacagtg 155220
gaaggggcaa ggcagctagc tttatggggt ctcatttata agggcactat ctcattaatg 155280
agggtcccac cctcatgacc taatcacctc tcaggagctc catgtcctaa tatcacattg 155340
gtgattatat tgtagcatat gaatttgggg gggacacaag cattcagaca gtaacacagg 155400
ccatctccct caagaaaccc tggttctagc tgtaatagtc tgtacttatc tgatctgccg 155460
ttccttctct gcctagatga catttattaa aatatctttt attatctaac agcacttccc 155520
tcttgcagtc ttccttaaac atcagcctgt attagtctgt tctcacactg ctataaagac 155580
atacctgaga ctggataatt tatggtgaaa cgaggtttaa ttgacccagg gttccacagc 155640
tggtatagga agcatggctg gagaggcctt aggaaactta caatcatggc agaaggtgaa 155700
gaggaagcag gcacaatctt cacatggcag ggcaggagga gatgggagtg ctacacactt 155760
ttaaacaatg agatctcgtg ataactctat caggagaaca gcaaggggga tgtctgcccc 155820
catgattcga tcacctccca tcaggcccct cctccaacaa cgggaattac aatttgacat 155880
gagattttgg tggggacaca gagtcaaatc atatcacaac ccaagttaga tttcccaact 155940
tgaattcctt taaagcatct gagatttact atagctgtgc tattctatta gactgcatta 156000
taatttttag ttggcttgtg tttttccttc tgaactgtga gctcttttta atttcaatat 156060
cactagtgct aagtaacata acctctcaga tgcttactga attgatttaa atcttctatt 156120
tactttttcc ttccacttaa aatagtagct cttttctttt tctttctttt tttttttttt 156180
ttttagagac agggtcttgc tatgttgcct aggcaggagt gcagtgactt tcacaggtgc 156240
aagcatagca ccctgtagcc ttgaactcct gggctcaaga agtcctccag cctcagcctc 156300
ctgagtagct ggaactacag gtgcacacac tgtgcctgtc ttatatttta ttgagtaatg 156360
tgttaggtac ttttatatac tttgtctagt ttaatcccac tggagcttag gaaacaagtt 156420
tagaggagtt aagtatcttg cccccatgtt tatatagcta ggaagttaac atagttcatt 156480
ttaaaacctg ggtcattcct gctcctattt atcttctcaa atattcagaa gatgttaaaa 156540
agtaaccagg tttttggaag atagctattt tgtttcttat ttttattgtg ttaatataga 156600
tccattagag taccagagta attcagcatt tggtttaaaa gtcttggctt tacttatatt 156660
gagagattca ttagcagaaa attacttatt gatagtgtct tatcctcttc aaaatcggaa 156720
ttaacaaaat gattctttta tttctctcac agtgattttt taaaatagat tttatttttt 156780
agagcaggtt tagattcaca gcaaaattga gaggaaggta cagagatgtt ccatatatcc 156840
cctgccccca catatctgca gcctctccca tgatcagtat cccccatcag aatggtacat 156900
ttgttacaac tgatgaacct acgttgacac atcattatca atcacccaga gaatgtagtt 156960
tacattaaag ttgacttttg gtatggtatt ttctatggat tttgacaaat gaataatgac 157020
atgtatccac cattattgta tcatacagag taatttcact gcccttaaaa tctttgccct 157080
gcttattcat cccaaactct tagcatccac tgatgttttg attgtctcca ctgttttata 157140
tttccccaga tatcagatag tgggagtcat ataatttgta gcttttttgg atgggttcct 157200
ttcacttagt aatatgcact taagtttcct ctatatcttt tcgcgtcttg atggcccatt 157260
ttttagtgct gaataatatt tcattgtcag aatgtacaac agtttatcca ttcacttact 157320
gaaggacatc ttggttgttt ctaagttttg gcaattatga ataaagctgc tttaggccag 157380
gtgtgttggc tcatgcctgt aatccccgca ctttgggagg ccaaggtggg tgggtcgctt 157440
gaggtcagga gtttgagacc agcctggcca acatggcaaa accctgtctc tactaaaaat 157500
acaaaaatta gctgggcgtg gtggcatgtg cctgtattcc cagctactta ggaggctgag 157560
gcatgagaat cacttgagtc caggaggcgg aggttgcagt gagccaagat cgtgtcactg 157620
cactccagcc ttggtgtcag agtgagtctc tgtctcaaac aaagaaaaaa tgaataaagc 157680
tgctataaac attgtgtgca gtttttatat ggacataagt tgtcaactct tctgggaggg 157740
caaaaggaag gtaattgcta gatcttttga taagatatgt ttagtttcag agtatgttca 157800
gattctgtca aactgtcttc caagtgactg tatcattttg catttgtatc cacaatgaat 157860
gagagttcct gttgctccgc atccttgcca acatttggtg ttagcagtgt tctggatttg 157920
gccattgtaa gaggttcgta gtggtatctc attgctttaa tttgcatttt cctgatgaca 157980
tatgatgtgg agcatatttt tatatgctta tttaccgttt gcatgtcttc tttggagagg 158040
tgtctgtcaa gggctttacc ccatttttta atcagattgt tttcttattg ttgagtttta 158100
atatttcttc atgtattttg gattatagat ctttatcaga catatctttt gcaagtattt 158160
tctttattgc tttttatttt cttgacaata tcttttgcag agcaaaattt tataatttta 158220
atgaaatcca gtttattgat tcttgtgttc atggagtgtg ccttttgtgt aatatctaaa 158280
aagtcattgt caaacctaag ggcatctaga ttttctccta tgttatctcc caggagttct 158340
gtagttttat gttttacatt gaggtctgag ccatttcgat ctaatttttg tcaagaatgt 158400
aaggtctgtg tttagatttg tttttttagc atgtggatgt ccagttgttc taccaccgtt 158460
ttttgaagat ggtctttgta ccatcatatt gctttcgctc cttggtcaaa gatccgatga 158520
ttgtattttt gtgggtctat ttctgggctc tttgttctgt tccattgatc tgtttgtctg 158580
ttctttcatc agtagtacat tgtcttgatt attgttgctt tgcagaaagc cttaaagttg 158640
agtagtgtca tccttctgac tttattcttc tcttttaata ttatgttggc tattctaggt 158700
cttttgcctt gccatgtaag ctttctaatc actttgttga tatccacaaa ataacttgct 158760
gggattttga tagggattgc attgaatcta tagatcaagt tggaaagaac tgacattttt 158820
acaatattga ggcttcctat cctaaatatg gaatatctct tcatttattt agttctttga 158880
tttcattcat tagagttttt ttagtttttt aaaaaatttt attttatttt tgctagaacc 158940
ataggcacac accatcacag tctgctagtt cttaaaacgt ttttatagag atgaggtctc 159000
cctttgttgc tcagtctgct cttgaacttc tgagttcaag tgatcctctc accttggcct 159060
cccaaagttc taggattaca ggcatgagcc atcaagcctg gctcttgtag tttattttct 159120
atagatcatg tacctatttt gttagggtta ttccttcatt ttgggaggtg ctaatgtaaa 159180
tggtattgtt gtaaatttca aattcctctt gttcattgct ggtatttgga aaagcagttg 159240
acttttatat attaattatg tatcctgaaa ccttgctaaa tagcttattt gttttaaggt 159300
tttttttttt tttgccaata ctcttgagtg ttttatgcag acatttattt catctgcgaa 159360
caaagacagt tttgtttctt ccttcccaat caatatacct attatttctt tttcttgtct 159420
tgtattaact aggacagggt ccccaacccc tgggccatga actggtactg gtctgtggct 159480
tgttaggaac caggccgcat agcaggaggt gagcggtggg tgagtgagcc aagcttcatc 159540
tgtgtttaca gctgctcccc cttgcttgca ttaccacctg agctccgcct cctgtcagat 159600
caggggcagc attagattct cacaggagca cgaaccctat tgggaactgt gcatgtgagg 159660
tatccaggtt gcctgatgat ctgtcactgt ctcccatcac ccccagatgg gaccttgtag 159720
ttgcaagaaa acaagctcag ggctcgcact gattttatat tatggtgagt tgtgtaacta 159780
tttcattata tattacaatg taataataga aataaagtgc acaataaatg taatgcgctt 159840
gaatcattct gaaaccatct cccacccaat ctgtggaaaa attacctttc cctcgtgcca 159900
aaacggttgg gggccactga actaggacat ccagtatgat attgaaaagg agtggtgagg 159960
ggacattttt gtctttgttc gtgattttag tgggaaagct tcgagtttct caccgttaag 160020
tatgatgtta gctatagatt tttgtagtct ttttaactag ttgaagttac tctatatctc 160080
gttcactgag atcttttatc atgaatgggt tttggatttt gtcaagtgtg tctgtcaata 160140
gatgcagaaa aatctattgc tatgattatg tgatttttct tctttagcct gttgatgtga 160200
tgaattacat taattgattt tcaaatgttg aaccagccat gcaatcagag gtaaatccca 160260
gttagtcatg gtgtataatt ctttttatac attgttatat ttgattttct atggatgatt 160320
ttatatctat gttcatgata aatattggtc aatagttttc ttgtgttgtc tttgtgtggt 160380
tttggtatta ggataatgct gacctcagaa tgagttagga agtatttcct cttgattcta 160440
atttctgaaa aagttatgga aaattgtcat aattaaaaaa aatatggtaa cattcaccag 160500
tgaaccaatc tgggcctggt gctttctgtt ttagaatgtt gctaattatt gattcaattt 160560
atttaataga tataggccta ttcacatcgt ctttttcttc ttacatgagt tttgtagatt 160620
gtttttcaag atttggttca tttcatctgg gttatgaaat ttgtgatttg ttagatcaat 160680
cagggataag aaaattaaaa agttatttta cttttattct tttatcaatc tttttttctt 160740
ttctttgttt ttcatatttt attttatttt gttttttgag gtggggtctt actctgtcac 160800
acaggctgga gtgcatggtc acagctcact gcagcctcga cctcctaggc acgggtgatc 160860
ctcctgagta actgggactg taggcactca ccaccacatg cagctaattt ttctattttt 160920
ggtagagagg ggattttgcc atgttgccca ggctggtctt gagctcctgg gctcaagtga 160980
tctgtctgcc tctgcctacc atagtgctag gattataggc atgagccacc ttgccctgct 161040
tctttctcta gatccaaatt tctgacctac atcattttta tcctatgtaa aggatttttt 161100
tttttaattt cttgcaaggt agatctactg gcaaaaaaat ctctcaattt ttgtttgttt 161160
gagaaagtat ttttccttca cttttgaaga atgattttgc gggttacaga attctatgtt 161220
gttgtttttt cttttttttt atcaacactt tattttactt taccactcgc ttgtatggtt 161280
tctagcagaa gtaaaatgta attcttatct ttgcttctgt ctagctaagg gttattcccc 161340
cctcaccccc cacctctggc ttctttcagg tttttttctt tatctttgac tttctgtagt 161400
ttgcatatgt catgcatagg tatagttttt ttggttttta tcctgcttgg cattcacagt 161460
ctcctgaatc ttttgattgg tatgtggcat aaagttaggg aaattttcag tgattactgc 161520
tctgtatatt tctgctgttc ctttatcttc tacttccgct tctagtattc tatcacatgt 161580
atgttacacc ttttgtaatc atctcattgt tcttgaatat tctgttgaga ggtttctttc 161640
gttttttttt tgtttttagt cttttttctt tgtgcttttc agttttgtac atttctattg 161700
tgatatcttc aagctcagag agagtctttg ctcagccatg tctagtctac tagtaagctt 161760
gtctaagtca ttcttcattt ccgttagtgt ttttgatttc tcttatatgt tttttgttct 161820
gttttagaat ttctttttgt ctgtttacat tgcccttctt ttcttacatg ctgtccattt 161880
tttccattgg cacccatagc atgttaatca ttgttgtttt aaattcccag tctgataatt 161940
cccacattct gccatatctg agtctggttc tgattcttgc tgtctcttca aattgtcttt 162000
gatgctttta gtacaccttg cacttttttc ttgctatcta gacacgaagt actgagaaac 162060
agaaactggt ataaataggt cttcagtgat gtggtattaa ggtgttgggg gagagggagt 162120
gttctgtaat ccactgatta ggtctcagtc ctttagtaag cctgtgctta ctgtgatcct 162180
cacaagttct tcttagtcct ctccccttca ggagggacta gaaagctaga cttggctgga 162240
gctgggcatt tgttttcacc caggtcattt aggctctggt gacaccccag caggttaggc 162300
tgtagttaac tagtttttcc tgagagcaga ctttaagaac agagtactct ggtgagtttc 162360
catatagttt cttttacctt tcccctactg gaagcatgag gggattttgc tccagtattc 162420
actgtgagaa ccaggtcaag ctcctggagg taaaaattca cgcaagtatg gggccccagt 162480
gactgagtca tcctggagtt tttaacttta ctctaaaagt tgtccacact gagcctccag 162540
caatacataa attacagttt agattttcct accctaacac tcgttcccgt ggaggtttgt 162600
ggttttctgc tcaggcaaat tgtgattttt ggtatctgcc agttggtgtc tccaatatta 162660
ggggcagtga tttgcctggt gacctcaatt ctcttacata tctaaaaaga atgatttgtt 162720
agtttgttta gttttttcac ttgttggtag gatggaatag caacttccaa gcttctcaga 162780
tgctgaactg gaaacctgaa gtccccacaa ccatttttag gggcaaaact gtaatgttca 162840
tttcatgagt attatatata atttccacct agaggtaaat ggtgtttttg ttattactgg 162900
ttgttacttg ttaaaggttt atgttgtggt tttcaaagaa atttcatgta tctgtgctct 162960
aattttcttt atggaaaaat ggaccaaaaa aatagagagg tatttaattg caatgaatgg 163020
gtgccattta cccctccctg caaaaagaac atttcagaat cattcagaat tgaatgataa 163080
gaaattctgt ttttattgta taaggcttat ggtggtctta gtgttacaga atagctaact 163140
ttttgaaatt tcattgtttg ttaatccatg gatttttaaa atcattttgc ctcccaatta 163200
tgaaaaataa ctttcacatt gtagaccatt tggaaaatac aatttaaaag tcacctgtat 163260
ctccttgtgt aatggttacc atagttaaca tgctagcata tgacttgcca atcttttttc 163320
tgtattggat tctcttttac ttacatgaaa ttatatcgta catatttttg tatcctactt 163380
tttaaaaaac acatgtgttt aatgaggtaa ttttttaaat ggctatgtaa tatttcattt 163440
tatggatata ctataagttg tatatatttt ttggctgagt acttagattt tatccaattt 163500
tttattcagt agtataaatg tattataact gtcaaataaa tgaacatctt tgggtgtaag 163560
tctttacctt cttttttggt attgcatttt aaaaaatgta gatctagttg atgaaggtgg 163620
aattttcggt cacagaatga gcattttacg aactccagac atatttttca acactgttct 163680
ctagagaaga gatatccatt agccttacta ccgcagtgtg tgagaatgag ggagacacac 163740
ccctgccact tgtgctttta gtatttccaa ttttagtttt gctaatttaa tggagattgg 163800
gatgacagct cattgtttta atatattttc tttgattata aacaaaatag aatttttttt 163860
atattttttt gattatttac tatttatatt gtctttatta tagacttcat gtttttcacg 163920
tatacttata tgagtttctt gtatattaag aattagctat aagggaactg tggaagtcaa 163980
gactacttta ccatgttcat aaaaactgat aaaaaatgaa tataactttc agatcttgag 164040
agcggatttt acagatgtca tgtgagttca ttgagtaata ttcttatgta ccactactga 164100
ctgcttggga ctttattact gtaaatagga aaaacaaaaa attaagattc tgatatgcta 164160
attattttgc ttatatggtg gtgaatatac ttttaaaatg aaacatgtat tgcatgcaac 164220
aatactattt tggtagattt ttagaaaagc aggtattatt acaggtaata ggcaataatg 164280
acttcgaagg tcttagggac tatgagtagg taaacacttt atgaccacga ccccacaaac 164340
accccgcagg agattatgtg gctgccattt gtaaacggcc attcattgtc ctgtctcttg 164400
cctttggctc tttccagttt ttcttcccaa atctttggac tagttcctgc agaaaatatg 164460
aattcacagt ggtgccctct gggcctgggc atctctgggc cggcagaccc tgagcagcga 164520
ctgctgctga gagcaggtgt gtgtacagat ggcatggtgg ggccagcctg ccttctgctt 164580
ggtgatgttg gagagggtga ttgataatag agcttacacc gagtaatggt ctgaggcaag 164640
gcgagcatga gtgcctggct aagtgaaagt catcgctggc agagtggata tgtacactta 164700
atttagtctg tagttttatt aaacttcctt attagaggag gccttatttt cttattaacc 164760
agaggtctcc atgctggctg tttcatcttg ttgcctcctc tcttaccgtg tatcctctca 164820
gggaggtgag gataattgag aaagggctaa aaacagctct cccgagtttg agaaggtttt 164880
cagttgtcaa tgcttacata atttagagag gactccaaag cattgtgaaa gaaagctcat 164940
tatgcgtctc agtctccttc cttgtctttt cttgtagttg tctccatagt gggatgtaca 165000
tatccctagg ctagatttgg agttgggtga gaagaaaata ttaaaaactt catttttatt 165060
tcattctttt ttatttccca ttctttttca tttctatttt atatatgtta tgtaatatac 165120
acaaccaatt tttctacagt aaagtatgta tataatttat aagcaaatat acataaattt 165180
ggtggtatgc acaaaagttt ttaattttta agttttcgca gtgtgcagtt taaaaatttt 165240
gtagaacttt tgtcttatag gatttgttgt ataggcatgg cagcttttgc ttaccctgaa 165300
tactaagaca ttttgacatt tacataagat tactatctac caattggagt gtttttctcc 165360
cccctctgag taaatcaggc ttaatagagt acatatgaag ggcataaagt caagggcaat 165420
tctcactatt tctttgccag gattttaaac tgaagttaaa catgtcaaaa tgactaaaga 165480
tttagagatg attggtagag aagtgttctc agtttgttac tcaacctcca agttaatgtg 165540
gaattttata actgttttag tcagctttct gttgggagct ccaaacatca taaaatcttg 165600
tgaattctgc ctactactaa tcaataagtg aaatgatagt aattatctct ggttttcttc 165660
atttttatat gctttgacaa taatgtttaa aaccattctg ggctttgatt tgggattcca 165720
cagaattttc ttctgggtca cagagggggt taattatttc tagaactctg aaattttgtg 165780
attcttgtgg ttcaaaatca caaaaaccaa agtgaaatca tggtagaaag cttgatggtg 165840
gggaatatca gctatggaaa gtaacagtaa ttatcagttt tgtacttttt ttctggaaaa 165900
tattatcttt tgaggcttca tttaatattt ttctttatca gcccagaaca ttaaaaaaaa 165960
accctaacct actggaaaaa tttggtaagc cagtgctttg agtggtcaca atgtagagaa 166020
aatgagcatt gtcatgtagt aaagttaaaa gacagttgtg gccgggtgcg gtggctcaca 166080
cttgtaatcc cagcactttg ggaggccgag gtgggtggat cacgacgtga ggagatcgag 166140
accatcctgg ctaacacggt gaaacccaat ctctactaaa aaaaaataca aaaaattagc 166200
cgggcatggt ggcaggtgcc aatagtccca gctactcggg aggctgaggc aggagaatgg 166260
cgtgaacctg ggaggcagag cttgcagtga gccgaggttg cgccactgca ctccagcctg 166320
ggcaacaaag cgagactctg tctcaaaaaa aaaaaaaaaa aaaaaaaaaa aagacagttg 166380
gggcttgcac atgagtaagg gacaggatct cagctctctc tgtggtgggt ggttcttcac 166440
agtctaagaa ggcctctcac ttttttcttc catctttgtt tccttttcaa atatttaatg 166500
tgtagaaatt tttattgccc tttctgctta gtcagaaata ctaaccattg ttgaaacttg 166560
taaccatttc tgataatttg cccagttgaa ataagataat tcttttattc aattccagga 166620
ctctgaaatc ctctgagacc aaacttatag aactgtcagt tgtctggact ttttgtgtgc 166680
attttatcaa gagggagaag aacagttact ttctcccttc cctcaaaaca gggcaaatga 166740
cagaattcta tttccagata gatatgtttc tttcagggac aaatgtccta tatttagttc 166800
tttttagtta atatacttta tattagccaa tgcctagcac tgcacacagc agcagccttg 166860
ctttctcgcc tatggctttc tgaaagttga gaccacagaa atacttttgc ctttccttgt 166920
catgtcttat ccacttaatg agaataactg aagtaatact aaaagctttc ataagacctg 166980
gttaaacttg accttgagat tgcttcactc agaaaagttc ttagtttaat taacaagtaa 167040
ctgataggag tcatgcttgg catgggtctt cttgagaact tgccagctac tttttttttt 167100
tttttaagac aagatctctc tgtgtcaccc aagctggagt gcagtgacat gatcatggct 167160
cactgcaacc tcgatctccc aggctcagag gatcctccca cttcagcctc ccgaggagct 167220
gggaccactg gcacacacta acacacccgg ctaactttgt gtgtgtgtgt gtgtgtatgt 167280
gtgtgtgtgt gtgtgtgtgt gtgtgtgtat gtaggtggtt ggtatagaag tgttctcagt 167340
ttgttactta acctccaagt ttatgtggag ttttataact atctttataa acaaacaaac 167400
aaacaaacaa acaagcaaac aaacaaacgt attttttgta gagacagggt ttcactatgt 167460
tgcccaggct ggatctcaaa ctcctgggct caagcagtcc tcctgcctcg gcctcccaaa 167520
gtgctgagat tacaggcatg agccactgtg cctggcccct gccagttact tttattaaag 167580
tcacagtgtt ctactctgga tctagggaga ccccctttgg ttccacagaa ggagaagaat 167640
gggcatctct acctgtgcat caggatgtca tttttttgag ttgcgtagag aaggttctac 167700
tcccacagct tgcatattgt ccatgtctgt attaatcttg ttatgaggca tcaactcagc 167760
atgggtaata ggtagttaca ttaatgccag ggctcaagga gtcccaccca tttctttcac 167820
caaatagccc acgaaagtga tgagagatag gatgaccaca tatataccgt aaacaccaga 167880
atgaaggcct ttgtcactgc tttccccctc agtgaaaaag taggaaatgt ttttaagtcc 167940
ctgctgttgt ttgaatattt gattctccaa acctcatgtt gaaatttggt tcctaatgtt 168000
ggaggagggg ccaaatggag gtgtttgggt catggtcagt agctccctac agtcctcaca 168060
gtaatgagtg ggttcttgct gtcttagttc tggagagagc tagtggttaa aaacagcctg 168120
gtacccccac atctctcttg ccctgtgacc cctgcacaca ctgactcccc ttccccttcc 168180
accatgagtg cagattctgg tgccatgggc cttgtacagc ctgcagaact atgaaccaaa 168240
caaacctctt ttctttataa actacccaca ttcaactatt cctttatagt gacacagact 168300
gactgagaca gtccctattt aatgcagtgc tgctgctttt ccagccagca attctgaaag 168360
atgtagatct agtatacata ttttaagact gtgtttttct gggaaaaaca agttgtctat 168420
ggccttatga actggatttt ctcatgcttc taaacaaact ttagcgctga tgggagtatc 168480
cagaattatt tcctaaaaaa tatctagggc ttttggtcca tcagccactg atagtttatt 168540
atttgttgga tgtctgccat atgtagcctc tctttttgtt acactcaggg gaaggtctca 168600
ccatcaaaat cattctttag gactgcttag gttagagatc cagctagatg ctctggatgg 168660
acatcatttt ctcatatcat tttatctttt agagaaatac acatcatttt ctccagatat 168720
caagttataa caggaagaag tgatatttat acattactag agagcttctg cttgttcatg 168780
tctggtaatt catttgcacc actagtttct tcatgcttgg catggatctg ttcctgctac 168840
ttccatcatt cttttgtttt attccttttt ttctttggcc aggcagatat actctggatt 168900
gtcagccatt ctgcagaatg ttgaaggcag ccattctggt ttttattgat ttttttgtta 168960
attgtagtaa aatatacata atataaaatt taacatttta tccattttta ccatttaatc 169020
attatttaag cgtatagttc agtgtcaata agtatattca cattgttatg taaccattac 169080
catgatttgt ctgcagagtt ttttcattat ccaaactaaa actctgtacc catgaaacag 169140
taactcccca ttcctccttt cttccaatcc ctggtaacga cgattctact ttctgcctct 169200
ctgaatttga ctgttccaga ttcctttata tcagtcgcct ttctatttca ctgatattta 169260
aaaaatagat ttgtaaaagc cattgccaca tttcttgaat gcatggctta agttaccagt 169320
tacatttgca tttcttctct tagctgttgt cccaaaggca ttccacattg gcctgctagg 169380
cataggagag caaagcatgg ggagacccca gaaatatgag ccctcatcct tttaataaag 169440
actgatttta agaagtcgtc taaaaaccaa gtcaactttc ccaccacatt tttatattgc 169500
tcagtggcag ttaccaaata gaatttcgtg ttactgggca ctcctaaggt tccagggctc 169560
cagagctttt agggttagac tgtgaacttt tgaagaaatt aggtagctaa gagcacctga 169620
aaatatttag tgaaggaaat gaaatccatt ctttagaaaa aaattagaag ttagcctaac 169680
agttagcaaa gacaatttcc tttggttgtg taaaactaga atacagactt tttattttga 169740
atttcttttt atctctgtcc atgggtttat gcttggattt gcaacaaaca acaacaaaaa 169800
actgtttgta tttttaatag cttttaaaaa gtattttaac aaaaatattt aaaattttac 169860
caggtattgc taaaaattaa ttctttgtaa gttgtgttaa attggaaggt agtatggtag 169920
aataagaatc acagcttgta attatataga atttgaagta aagctgagta aatctttcat 169980
taacttttta caagtagcct atgaccctgt ttgctgctca tttctttcca gctttcccca 170040
gttaggactt gatgcaaaca gtatgtgttg aagaatgttt tgtatgacgt gttcggtagt 170100
catttttcat catgttcact tgtaatgaat ttcagcattt tccacattgg ctcacgaaga 170160
ttaaaaaaaa tcctatgtgt tgctttacca catacttcac tactgagtga taatctgacc 170220
tttgggagca ttgctggtca ctatatagaa tgatatttcc ttgttctatt tcattcaaaa 170280
tgaaaaaaat cattagagag atgcaaaagg aagaaaattg gctgttcttt tctagggctg 170340
gatgtactgg aaactacaag atgtcatgca aaaactagtg tgaagatcct gtaaaacatg 170400
actgaaataa actgattttt taaaacaaac tttattgaga tgagcaaacc attagtattt 170460
ctaggtaaaa tggtttcaaa ttaaaaaatg catagagcat cattgttgga aaataatggc 170520
ttcttaaagt tttggtttct gcttttggtg agatgtttga aatgtcaagg aaattgtatc 170580
tcctactagt taagatttta tctgtttttg aaagataatg tgataataat gtgataattt 170640
agatcaaaag agaaggccac tttttttggg aacatataca ttgtataatt taagcaaaaa 170700
cttaattaag aaaaaaatca ttggtggact tgattttttc aggactctgg ttcttgatta 170760
tttttgaggt acagaccatt ttaataatct gataaaagcc atatacatcc tgtccaccaa 170820
aaggaagcac attcataaac atggcaaaag ttttcatacc gtttcaattg gatttctgga 170880
aggccaccca tggatgcaaa attagaaaat gtttctgtag ggactgtcat gaaatgacat 170940
aggtaacttt tgtcattcat aatggttagt ttctgccaaa caagtttcag acgagtgagc 171000
tacttttcta caagactcag gccccattct gctacacaaa gatgcctagt aatcattgct 171060
atctcctggt atgggtttct gatgagagtg tcctgggctt tgtctttttg actgaagtaa 171120
ttgattgccc ctagatctga agtttgagtt acgtgcatct cattctttat gcacaagtat 171180
tttaaagtaa ctacagacat tgtaacagga gataatttat atgctttata tcagtgaggc 171240
accttgtttt atacgcaaat ccttttaatt attttcctac caatggtggg ttttagtcag 171300
ttttgtgatg ttccaatgta ttcacattga ctttgtctta tggtaaggtt tcctgtgtat 171360
tgcagtgtgt ctgtctgtaa catagtggct agtcaatgaa ttttggttga atgaacattc 171420
aaaatcccca ctgaacaaaa ttttagacaa cgttttgatt ttagaataat cttcaactta 171480
cagaaaagtt gcaaaaaagt tgcaaagaaa cctcttaccc agtttctcac tgttgttaaa 171540
taacatccta cattattatg ctacatttct cacaaccaag aaattaacat tggtacattc 171600
ccgttaactc aatgccacat tttattcaga tttcactagt tttcccctaa agtccttttt 171660
ctgttccagt atttcatcca gggtactatg ttgcgcttat catgtcttag tcttctctag 171720
tctgtgacag tttctcagat tatctttgtt tttgatcctt ggcagttttg ggctggtcag 171780
ctgttttgta gaatgcccct cagtttgggt ttgtttgata attttctcat gttgagagtt 171840
atggactttt gggggaaggc cacggtggtg aagtgccatt ctcatcacag catatcaggg 171900
atacctacta aacaacttta tttttaatat ttcagtatca caatggagag acgggaagag 171960
taagaaggta aaatttaaag gagtcagtct tgtcatggta aaaattgtgg taaagataag 172020
gtgagggaac tgaatctaaa agaatcattt atatttatct gggggaattc agcccttggt 172080
gttcctgtca gtgactcact ttgggtgttt aagagttggt agaatttatt ctatttaaat 172140
gatacaaatc agaccaatga aaaggtcagt ggttcataga tacacatcaa ggcagaattt 172200
acaatgataa gttttaaaaa ctctcttgta taaccatttt tttaaagtca gaaaattctt 172260
aattgtattt ttcccctttt cctctgtttc tatatatttt tcttgacctg tgttcttttg 172320
cattaggatt tatgtttttc aagtccagtt aaggatattg agcaatatgt caaaacattt 172380
gtcacctgaa tggtttatcc cttaataaat ggcgaggtgg ttttgcagac aggtgacaca 172440
ctttaatgta ttacagagac cagacttgtt agttattgat tcgtgatgaa taaattactg 172500
ccattctaat cataagaaaa tttcttttgg ggataattaa tcagattcat gtgcttctta 172560
agcagtgtag cattatcatc ttttttagtt acaagaatga acctgtctgc ctctgccctt 172620
ggtaaattta gggcagaggc agacaggttt ctaggtttgt attttgatct ttatttttat 172680
taagcagatg agaagcatac tcgcttaaaa attacttcaa actgcctttc ctgttgagta 172740
ggttttgaac tacctgcttg acagatggtg ttagaagtaa ttactgaaat catgtgttta 172800
cctgctcatg gacatgtgtt caccagatgc accctgttta cccagggctg ctgatttctt 172860
aaagaaatat gcaccttctg gatagagttg cataacttag gagaataaaa gctcgtcaag 172920
ccggagctct caatctctgc atggcagaat cagctgtagc gagaagacca tttacatttc 172980
ttaatgagtg gtcaatttag atcacagaac catgtacagc attatttacc tttcttcttt 173040
tgatttactt agtagtttaa ttttacctgt ctactgagtt atttaacaga tagcctccca 173100
aattgttgac taaattgtag cagtagtgac taaccagtct tttaagatga tggtgttttg 173160
gtttattatg ttatttttga aaacatgttt ttcagaagac atattaaaaa tgtacccatc 173220
cctccccgtt tctaaattct gattgtggag gtccggcttt tggattaggc attttttttt 173280
tttaacacct catgagtaat tcttatgttc attaagatgg agagtagtta ctttaatatt 173340
ttatttacta actatcaaaa ctgaatttat tttttccacc ttgcttctgt tttattctgt 173400
tttttaaaaa gttaaactga tgagccaggc atggtggcac atgcctgtaa tcccagctac 173460
tcaggaggct gaggcaggag aatcacttga acctgggagc cggaggttgc ggtgagctga 173520
gattgcacca ttgcactcca gcctgggcaa caagagtgaa actccatctc aaaaaaaaaa 173580
aaaaaaataa ataaaagtta aactgaatga tagtgactcc cctaacatgt gtgccttaaa 173640
tgttcaattt tgaaaaggag tacatttaac tctctataaa cctagagaaa caaggaaagt 173700
cattgtttca ggattttgac aggtgcaaaa ctgtaaataa ttacaggcct gggcagaaac 173760
tagaacagaa gttgcacagc agctggcagg cctgacctgt gacaggtctg acctgagtta 173820
agtagcctaa atttcatggc aagctcagat ttgttttatg gtatgaaatt caccttctgc 173880
tacagaaaga ttctactgat tgaggctaga tatttgccaa aaagggagta acatacagaa 173940
tattgagaaa gaaaactgcc cactgagcca cctcttgtat gacttcagga ccagatggaa 174000
ctagtgtaga tgcattttaa aatcaattta catttggact gaaataaatc actcaacacc 174060
aaatggtata cagtggttat agctgaggag ggtggtctgg aggtttttgg gttttttttt 174120
ttttttacac tttttatgta atctgtttct aaaacatttt aatttttaga cttaggagca 174180
tatctgactt tagtgaagag gaaagcagtt ttaatttaat atcttgcgaa tacctgaatt 174240
gtatttttca aggtctttgt agagaataat acaaaatttc gatgagattt tatcaccttc 174300
aggttccatt aagcagttct ccaccttagg acaccagatt tgttcctggt ccataaagaa 174360
gccggctagc agcttccatc cccacatgct gtgttgcgac cctggaaagc tgcagtttag 174420
aaaggatagg gttctgatga cttatgggct caatctcacc tatacatgaa gttgtttttt 174480
tttttttttt tggcttgccc agtgtcttga aaatttgaca atttcacatt ttaaaaaatc 174540
tgtgtttcta gctttcttgg aaaaaccgat gaatggcagt gctgggcctg cactgtacta 174600
ttgctggaac caagtagtag ctctctgctt ttagactctc tgatttgctc cagtccttat 174660
cattccctgt tgtactttca tgtctattac cttcctgtag atacatgagt taatgacccc 174720
tcgagtagag gatatgtgcc tcagaagtgg agacatgcag agagaagaca gctttcagga 174780
ggatgcttgt ggaaccttta tacagttagg cttggtgtgc ccatgtcttc tgccccctcc 174840
cactctgtct aaggtttttc cctttttttc tacgtaatac ctcaattttc cccttcacca 174900
ttagggaaat tatacctacc agaactttga gttatgttcc cccagccacc cttagtaaat 174960
atcctatgta cctggtgtgt aggagttttg tgaatgttta ctagatcttt aatctaccca 175020
tttgtatgtg tagtttaatc atctagtttt tcttcttgtt tttagtagaa atcagctttc 175080
ttgaaggata agagattgat tagaaaagga atggagacat ttttaaaggt ctgggcagat 175140
tgagagttag ggcagatcct gctgtgatgc cgtggtggga gaggctgaca gcaatgggat 175200
cctttctagc atggacagga ggaagcggaa agatgccagg gattaaattt tctcagaaaa 175260
ggagaagaaa tgatcctctt cttccttctt ttcttcccta ctcctgtgct gtttggtgtt 175320
taaactctta gcactcagtg aaatgggaaa ggttcccttg tccccctcgc agggcgtgtg 175380
atgtgtgtgt ggctcacttc ttcagtctcc cactgctcga acctctaggg gagcatacag 175440
acgggcaggt cgtggggctc tgaccccatg gcagtgtcta agggtggatg cttacagctc 175500
ctgaagcccc agtaggcgtg tgttacaggg tgctttttta gtctttccgt ctataggctt 175560
gtgttaacca gctgaattag accctctccc ttgtcacaag gacagagggc tttctgtatc 175620
ccggattctt gccttggtgt accggaagaa tcagatcaca cctgggcttg gggaatgagt 175680
gcaaggttta attgagtgga agtagctttc agcagatggg ggagccagaa gggagatggt 175740
tttcttgtgg attgggcctt ccaactgctc aggcaaaact tgtcgttccg ccagtcgatg 175800
gccggctggc ctgctggcct gctggtgtct gttgtgtgcc cctctggacg tcctctcgat 175860
atccagccac ctgtgttttc ttcgccgatg tgttcctcat gatgtccagc cacttgtgtg 175920
ttggcctgct agggtctcgg ggtttttata ggcacacgat ggggtgtggc cggccagggt 175980
ggtcttggga aatgtaacat ttggatgtga aggcaggagt gcctgtcctc acctaggtct 176040
gtgggggtgg agccctagcc aggggccacg cccttctcta cccagcactt cccttcacca 176100
cttctttatc atttgaaggg accacgctct tcccttccca gcactcccgt atcactaggg 176160
tctgtggcag aaataggatt tccctttgct tctaattggg tatagagaga agtgaagagc 176220
tgagtttcat ttgtctaaga atctcacaac ttactgtaat atggtttcca ttagaatatg 176280
tgttgcaaat atagtgaaac ttaaaaacaa aacctccatt gtaagttgac ttttggcacg 176340
tagcttcctc atagcttggg ttgttcttgg ctttgttaaa taaagttaaa ataaagaaag 176400
tggtgtagac tggggcttgg caaacaatgg ccctcaggac aaatctagcc tgttgcctgt 176460
ttttataaat aaagttttat cggaacagag ttgctctagt taattaaggt actgtttgtg 176520
actgcattca caatattagt gtagagttga gtagttacga tagagactat atggctcaca 176580
aaatctaaaa tatttattat ctggcacttt acataaacaa ttttgctgac ctttggtgta 176640
gatggttaat atccaaaaaa caaaaaggaa aaaaaaaccc aactctttta tgtgctctaa 176700
tgtatttgct gttgaaaaca aagggaattt gtgattcatt taaaaaatat atatatagct 176760
atttgatgat tttgaagata ttttatagtt attgttccat ttgaatgaag taaaaatgtt 176820
atgatgtctt agggtacaatt gagctataa ttttagtatg tcaatgacta cagctttttt 176880
ctagggaagg ttataatagt taacaggaaa atatgacata aatagtaaaa acatgatata 176940
atagtaaaaa ttagtaaaaa atttttgttt tgggctgtaa gtttttttgg agaggagact 177000
gtagtaagtg taaaaacatg atgttatata attagtttta cacatatttt caggaatatt 177060
tttaatacac aaatgtgtgg atatagtcta ttttggtgtg aatttaaact ttgaaaactg 177120
ctaaaagttt cagttacttg agaaatagtg aatttttatt taatgatcaa attaaacact 177180
aataaaatta tgtctttatt gttttcagtt ttgagagcat ttgaaaagtg caggcctgtg 177240
ttggggattt cattcaacac aattattgat tgtttcaggt attaagcact gtttgaggct 177300
ctgggatttc agtactgaat aaaacaaaat acttaatgag cttatgtggg tagggagaga 177360
aacaaacaaa cattccttga ctcaattttt ttatcaaatt acaccaagtg cttttttaag 177420
acctggggat cgataatagc aatgaaggaa gcaaattttc tgctgtcatg tagcttaaag 177480
aaaggtaaca tgtaagtctg gtagcaactg ttaggaagaa aatgaaagca aggcattgag 177540
agagaagagg ggtgtaattt tttgtagcgt agtcaaggaa gacttctctg gtaaaatgat 177600
ttgagcaggg acctgaatga aataaggtaa tgagtaatgg gaatatttga gaaaagaaca 177660
ttctagacag aggaaacagc aataaaaaaa acactggggg aggaatgtgc ttgtcatgtt 177720
caagaaacag caaggagagg caagtgtggc tggaatagaa agtgccagag ggagaatggt 177780
aggagatgag tttattttct acttaatgaa tagctttgaa tatgtatcag aggcaaagcc 177840
acatgagacc aagctaaatt ctgtatgagg tgacagcagt ataaaaccag aaaacaagga 177900
tatatggaag aggtccctag accagaagtg aggaaataat agttaatttg ctcattgttt 177960
ctcaaattaa atcccacttc tgtgaatctc tttgtgattt actctttcaa aatggaattc 178020
tagatggtat agtccagtgc cccgaggcct gtgtaaattg ttgaatttat ggtgtgccaa 178080
taccattata gggtggtcat ctggttaatt tgcaacaaag aatatgaggt ttggaataaa 178140
tgggtcagaa agcctgacta aaatgagaat aacaattcac attcaattaa aaatgtgagt 178200
agcctgcaat aatttcttat tgtacttggg taaaggggag aatagaagta gaatgataat 178260
taatagttgt aaatttaaaa gtcagctttt ttgcaagtgt taatttttcc tggaagtgtt 178320
gtacaaaatg atcaagttcc tgtaattgca gaaacactga tcatggtatt tataattgca 178380
aggcatgcga ggtagctgac cttaagtgta aactaattaa cgtaggattt gtgtgatatg 178440
ctttagcaaa agtagatatt tgaaaataac tgactttttt tcattgtaca aatgacaata 178500
tttgtggcat tttatttgtt taatattact gagcacaatt ttcactgtga ttcttttcat 178560
tttaaagcaa gtatcaataa atgaaatttt gtttctggca taataagatt attacttaag 178620
cttgttcaca aaatatttta taaagaacag catcaatcac aactattatt tagctactgt 178680
ttgtggaaga gtacaggctt ttgacactca gcagttagta ataattattt aaataccata 178740
taaagacaag acaaattaaa tgtataccat tcagacattc ggagatctca ccaggaaaga 178800
cccccatgta taaacaactg ggcctaagaa gctcttttgc aaagacttgg tggaattcag 178860
gacacatttt tactcaggag aaccatatat agggtattca cttcacaggc aaatgcaaaa 178920
tgtatcagga agctgtatac atttcatgga ttttagagga gtctagtact agtttttagc 178980
agatctaact ttatagaaaa gcttatagct ttccctttcc ttggtgtata tatatcagta 179040
gcacagccat tctcgaatct gtttaggatt actcttctgt ttgctacctg tgtcacttgt 179100
cctgtgtggt gggtctggta gtatgtgttc atagcggggt tgctttatga gtaggtccac 179160
tggtgaggca gttattgaca ttgcagtcac tcttccaagg gcagcagcag accgtctcca 179220
tgggtgctgt gcttatgatt ttcagccaca gaaagccaag gtgatgatga gggccgtcct 179280
tgaccacgca tttggtccac acttcacagg acaataggag aggtggggaa ggccaggcac 179340
agcagactgg gacaggcagg ccctctctat ggaaagccct atggtgctac ccatcaagat 179400
tcctttgcct tcattctgct gctgtatcca gagataccca tgcctctcac tagccttgcc 179460
tggtatgggg aggcaaacct gtttggggct ggattttggc tgtagggttg tgtgaaatag 179520
ggtgaaatag aagaagggga agaaaaaatc tctcagtcta acttcttttc tttactttag 179580
gcttcccgcc aaccctttgg tgaatttgaa agtagtgatc agatgctcat atgctgatcc 179640
aactagccta gtttccaccc ttacattttg agtaaactac tacaatccag actttaatgg 179700
cactggacac acctttctcc tcactaccat tttagattta taacatttta cttttattta 179760
ctcaagccaa agtactcatt taaggtgtcc tctaacttta caactaggga gatggaatac 179820
catcaaatat ataactacac ttaaatatat ctgtgtctat ctgtatttgt gtgtatcatt 179880
taaatatctg ttatacaagg tcataaataa tccaggagta cagcatctgt ttcacctgct 179940
gctctcttta tgatgtgaag tgatgagtca catgagtaat tgtattactt gtataaagta 180000
cttggaagac agtttaactt gcataagtcc tgtgctgtgt gtcataaaga tgaaaagttt 180060
gagccatctg ttacttaaaa aaaaaattac cccagtgtac cacctggtgt ttaactaggc 180120
cttgggattt gttccagata cagcactgct tcattgaaca aatattgggg gtgagggtgg 180180
gtggggtttg gtgattactt ctgggacctg ggagggaccc ttttcactga gtcctgaaca 180240
acgttattta gatcattgtt atttcctatt gtggtttgtc tgtcattaaa aacaattttg 180300
tcttaatttt gaaaattgga tatgaggagt tacattacac aaaacaacat tatacaatga 180360
tggagataat gttctgtgca gcatgattta atttggagca gtggatagcc acctgctagg 180420
cctttgttct tgtggcagtc tggcaagtgg tcccagtgtt ttgggtcttt ccttggtcac 180480
gtggctgttg agatctgcct gggttagttg caaaccatta atccagtagc acagaatgat 180540
gaaatatttg actactgggt gtttgaagta agaaaacaaa tccttttaaa agttattatt 180600
cagcaaccac agaatgcttt tatcatgttg atataacatc tgagtcttta cattttatct 180660
aagggtagcc tattcttatc tacaacataa aaaagaaatc taggtaatat ttggcattat 180720
ggtaactagc taccatatag cacctgtctg cttacccact gtggtggggt aaccatataa 180780
ttcagtgatt aaactggaat acttttgtca gtgacagaga gctaggacga aaggtgtaaa 180840
caaggatatg tagtcatttt acttacaagg catagccata ttttgccatc ttccatgatc 180900
aagaataact gactattcct catgcatgta agagtaattc ttattttcaa attgcattaa 180960
ttgtgctggt atagtgcctc atataaagta ggtactcaat aaatatttgt aaattgaata 181020
aatgtttatg cttaaagaaa tcttaaagct tcctgccaaa atctttaaaa atgtacgttt 181080
tagaagggct ttgagttaaa catcctctgt attttcttga tattaagacc cagtgtcagg 181140
attccccaca gtggcattac ccatggacct gtgatctaaa ctctgaaacc attttaatat 181200
gtagtatatt ataagagtag gaaaagcact taattgcaat tacgtattgt tctgtctaaa 181260
ataatttaac tgtgtgctag aagagtgagc cctttagtca agtgcttatg acttatcttg 181320
gtaacttatt tactttacct ttttacttga agttgttaaa caattcagtg gctacttaac 181380
atttccttaa atttactctg agagaaaata aggaacccaa gtcatttaat ttacttgttt 181440
gttagtacct tgtcagagac tttaatagta cggaaagaaa tttgggataa tttttttcac 181500
gaattttcaa tttttgtgtt gttcacttta gaaattttct ttcatttaaa gttttctctc 181560
tccttgcaag gcctacttta ctatatatga catagaaaag ggtgcaggaa tttctatcct 181620
tttctatgtg gtgattcttg cctgcctggg cagcttatga gacagattgg atttagctca 181680
accctggaat gtgtgttaca gtgtggtggg ttttggcaag tcacttgtgg tttagaggga 181740
ctctatgacg tgggctggga actttggtct tttcttttgg tttgaagtag gttttttaaa 181800
aaaggtgtta gacagtttca ggttcgagac cttgttaatt ggtttaacat cccatctgtc 181860
ctagtcactc tgattctaat atgacagaat tgaaacagat gctatttaaa tctccatttc 181920
tttattttat tttagttttt tgtatgtgtg catgtgtctg atgatattgt agtcttggtc 181980
tttctgtatc tttccctttg acttttctac aatttctggt ctggtttctt tgtgtttcag 182040
cctttttctt cttgtcctta tgttttttct ttttgacttt tttcacttga gtgacagagt 182100
ttaccaagtc ttattgttta tgtctaccct gatgtatcat aatagtcaaa cattggtcat 182160
gatagaactt gcttcagtac ttcatgattt ttatacagta gaactaaatc catgggaaga 182220
tatttattta cttgttaatt atcttcccat tgcaatgtaa gctccaaggg caggggttct 182280
ctttttttta cgactgtatc cccagagcct ggaagtgtgc tgagtgtcta acagctgtta 182340
aaaaaaaaat gaatagaaat gctttagttt tctgagagtt tcccaaatta gatattattt 182400
agggagagtc ttgaagttcc tttgctctac agcacacccc tgaaccttgg ctggtatttg 182460
aaccgtctaa tgaagaacaa agtacttttg agagattcta tttgtaatac tgtaagattt 182520
tttttttaat agattgagca tccctaaccc aaaaatccaa aatctgaaat gctctaaaat 182580
tcacaacatt ttgagcacca atgtgatgct caaaggaaat actcattgga gcattttcaa 182640
ttttggattt tccaattagg gatgcaaaaa aaaaatttga agtctgaaca cttccacttc 182700
caagcatttc agataaggga tcctcaacct gtagtctctt ccctttctag tgctcattgt 182760
ccctagactc gattttacta ctttatcagt taagatgctt gttggtttca ttaatagaaa 182820
tctcagctga aactcactta acaataagga cgtggattat gttgcacaac aggaagtaca 182880
agaaggcttc agagtgggtt aattgaactc attgttgtca taagagacct aggttgggtc 182940
tttttatatt tttcttttgc tttcttggct tcatccttca agctattatt ttctgtgatt 183000
attaggacaa gggcagcaat tagatcaaca ttttccttgt tgtatgtaat gggaaagagg 183060
ctggcatttc tttccttgaa gacctgagta agaatttctc tatgttttta ttgggccaaa 183120
ttggtctcag attattctct cccgagtatt ggcaaaggca atggagttat agcgattagc 183180
ttggagtaat catcgagagt gaaatggatg ttggaatcaa ctacaacact tgcagaaaat 183240
cccatatgaa ttaaccttta taaataaatt tctaaagact gttgatttcc tgagcttcaa 183300
gttaaaactt ccctgagtaa ttaggtttat aaccaagagg atgcagggtg aagtcaacat 183360
tatgataatt acttcattag ggaaatcatg gcatttaaat aaaaaaagta acaagtttca 183420
tcttcctaaa tttagtaaat attacatgca acattgtttg tagaaagtag gtacagtcat 183480
gtgtcccatg atcccgtgat gtttcagcca ttgagagcca catacacatc tagctatgtt 183540
tagaaacaca aatacttagc tttgtgttat agttgcctac agtgtttagt acagtaacat 183600
gctgtataga tgtgtagcct aggagcaata ggctacacta tatagtctag gtgtgcagta 183660
ggctgtacca tctagatttg tgtacataca ctctgtgata ttcacacagt gatgaaattg 183720
cctaatgaca catttcttaa aatgtatccg catcattaag tgacgaatga ctgtatgtaa 183780
aaatcagtta gaactgactt ccctttaagt ctgtttaaac tgataacaat cagtaagttt 183840
gaattattta aataaaatga aatctgtgga ctagaaaagt ggaggagata actaggacat 183900
agtgaagctt actttaaaaa atacagagtc aatataaaag gtaatgtgca tctgttacct 183960
taatttactg agaagttttt gatttacttg aacatgcagt aatggattat ttttgtttgg 184020
ccaagtattt tcagtaaatt ttaataggca tttggaagca ttatgatatt atggagaaaa 184080
tactgaatca ggaattggaa tccaaatttc tggttctggt cctacctatt gacatgattt 184140
ctagtaaaac attcaacctc tgtaagccct catttctttg taaaatgctt cctttttaat 184200
tttcttaaaa tgtctatttc ttttcattga ttgtaaccac tgctttcctg ggaattgagc 184260
gacatatatc attcctctag aggtgtggct ttttagtagg attaagatta tatgtaatta 184320
agtttatttg aacaagaagg aacaggtaaa aagcaaataa aagttgtgag atagatatca 184380
gttctgtcct cggtgtatac agtgattgta gagaagagta tttctgtttt gaaatataca 184440
tgttaattca ttagaaagga attattttgt aggtataaac acattacatt ttcccaatat 184500
ttcgagtatg tcattagcca aatatgagat tctaatgttg tctggcattt cgaatagtgt 184560
cagacttcta catttgtaaa taaaagactg aagcatagat tccttaatca cctgtaatat 184620
agtaaaaaga gttttggact tagagtcaga agacaaattg gaatcctggc tcagctagtt 184680
tagaaaaaac cttggtagca tggcggaatg ataatatgga tgctggagtc aaacagaggc 184740
aggaatgagt tggatttcac cacttacatt aatacatttt gatgaaagaa agtttggata 184800
cctttggcct gtatgttgca gaaaacaacc agaagtagca tgaatcataa gtgtgttatc 184860
tcatagaaca ggaagtctgg aaattggtat ttatgtgtta attccatggc ttaatgatat 184920
cttctgcaac acaggagtat ttaaactttt ggttctgtca ttcttagttt gtttgtggta 184980
tcttcgtttg agtggtgata ttgctgtaat agcaatagtt caagatatta catgcagtca 185040
tgatgacagc cagtaaagag aggaattcct cccatttgtc tctttttagt aggtagaaaa 185100
ccattctcaa aagctgtagt acacaccatt gattcaaatt atgtctcaaa ctcagactaa 185160
aagttactca ctagcaagga taatgaagat gccatgactg acttagactt ttttttttaa 185220
ccccaccttt ggatttgggg gtggggaggc tctttcctga gcacagcccc tcatagggta 185280
aacaacaaac aaaatcggat ctctgccagg aagcaaatgg ggtgtgtagg gagaagaacc 185340
tgagttggca aacaaaagtg atgagctaat catcttgagt agaggccagt aggagatgca 185400
cagaaaccaa gagagagtgg taacagaata caacagtctg tgctcggggc agtatgcagg 185460
gtgctacagg agaatgtatg agaaaacctg aatccaaagt tgcaatggtc aggaaagact 185520
tttctggagg aaatgacagc ttagttatca cctaagggat tagtggaaac cagacaggaa 185580
ttagtcatga gcagcatacc cttcttctgt atctataaga gcacaaaact tagccatgat 185640
ggtgtttcag gactatttta aggaaagttt taggacaaat gttggcttca atacctaatc 185700
agtgtgcttg atgtgttaga aaaaaaaaaa agaagtgaaa tgaaaagaaa agaaaattta 185760
aaaaaagaaa agaaaaaaac acctaatcag aggtaagcac cattatctgt atgtataaca 185820
actaatgttc aaaatgattg ttgtttttca agaaaaagtt atataacttt tttttttgag 185880
atggagtctc actctgtcgc ccatgctgga gcgcaatggc acaatctcgg ctcactgcaa 185940
cctccacctc ctgggatcaa gtcgttctcc tgcctcagcc tctgaagtag ctgggattac 186000
aggcacccgc caccatgccc agctaagttt ttgtattttt agtagagggt ttcaccatgt 186060
tggccaggct ggtcttgaac tcctgacctc gtgatccacc tgcctcaccc tcccaaagtg 186120
ctgggattac aggcctgagc catcatgcct ggcctatata actttaaaaa tgctgtatat 186180
tacagtatga aacactctct tttaatcttt gtaagattct aagattcaaa ggacttgtga 186240
tcatgtagtt cttacttctg tgaaaagcaa tcattactta cttgatgcct gtaatgagaa 186300
gtgtttatta gaacaaaaac taggatgact ttaattattt caagaagcac aggtaagaga 186360
ctttaccctc tgatgttaat gcaaagcata tacctggaat cgacattctg ccatatcttt 186420
acttgcatcc tttaaagctg tttcttaagt tttgattgaa ttaattaatt tagaaaagag 186480
cttacactaa caagggtttt tttcttctaa tgaatcagga gtgagtttgc atgttttgct 186540
ctccactgcc atttttatat gttttgtttc tctttaaata atactcgtat cttaattatt 186600
taaaaatgat accacatcct aagagtacat gatgtaaatc attcttagtt cctaatactt 186660
actctccact ctccaaagac atagacatca tattaggaga ctgatattct tttttcatat 186720
tacttggata attcattgcc ccatatattc atggagaata attgcaaggc aaaccaacag 186780
atatttcagg taacccagaa aaaggatatg gtaagcacat ttgcaaagaa atacttgatg 186840
aactagaatt catacactgt acaatgaaaa taaagatttt tatgatcgat tttgacttaa 186900
aatttttctt ttttttcttg ttaaaaacaa aacaacatct aagctcaaga aatataatac 186960
ttaacaattg acatagaaaa gtcagacaag ttctttattt atagagcttt ctaaatacct 187020
tatcacaagt taccacaaga taaatgtgta tgaaaaatta gagccaccta cctgttttgg 187080
tttttgtaaa aatcaaatgt gagacatata ctttattcac tctattaaaa tacatcagtg 187140
atgctaactt tgatcatatt tggactttta aacaaactat tttatcctta aaaaaagaaa 187200
acataaatgc aagcaaaatg atttcttatg acacttttta aaaatgaaaa atgcttttgt 187260
caagatagac ttatacttaa gaaataaaga aaaagcagga tctatcgacc aggatgaaag 187320
atgttctttt ttttatcata ataacatgaa gggcaaatta ccaccagctt atgggcaaga 187380
agtaaataaa aacaaagtac tatgatacaa aaaattctca taatgttcag ttgatccaga 187440
agataatagt ctaaggctat tagctatgct agtctcttat ttttaatatt aaaatatttt 187500
ttattaaatt ttttgcatgt taagtgtttg tcgtaatcaa tgatgtccca aattcaatgc 187560
ttttgttaat attaatgata ctattgtctg ttcttttttt tttttttttt tttttttttt 187620
tgagacagaa tctcactctg tcacccagac tggagtgtag tggtacaatc atagttcact 187680
gcagccttga tttcccaggc tcaagtgatc ctcccacttc agcctcccaa gtagctgaca 187740
ctacaggtgc gcacccacca tgcctggcta aattttttat tatttgtaga gactgggtct 187800
tccaatgttg tccaaactgt attattgtct attcttgttc actagcctga gccattcacc 187860
tgatatgggt gacatttttt cttttttcac tgaaatgaaa attggcagac ttttgctgct 187920
tctttattac ccaatgttaa ctaatgtttt ccagtctaga attttaactt aactaatatt 187980
ttaaacttgt atattaaaat aaaacacttg gcatacataa gagtttatag agtataacct 188040
ctacatctga agaattaggg atacttctga aggtgaggaa atcactttgc atcatgctat 188100
gaatttgtta aactgttttt attttgtgac tagcagcctt gttttattta aaaaataaat 188160
tttattgtat aaatttaaga tacgtgatca gacacatata tagtaaaaat attattatag 188220
tggaacaaat taagatcatc atcttacaat tattcatttt tccctgcctg tggcaagagc 188280
agttataatc tactcattta gcaaaaattc tgaatataat acaacattat taactatagt 188340
ccttatgtta tacattgcat ctttcaactt gttcatcctg ttgtgtttgc tgctttgtat 188400
cctttgacct acatctccct attttctccc acttctgcca gaatcactgt tttatccttt 188460
atgtattat atttgccaacc ctccccccac ctttttaaaa aagattccag atttaagtga 188520
gatgatgcaa tatttttctt tctgtgtctg gcttatttta catagcctaa tatccttcag 188580
gcccatccat gacgggatct tctccttctt aaagactgaa taatagtctg ttgtatgtat 188640
tcaccacatt tactctatcc atttgtctgt ccatagacac ttaagttgtt tccatttctt 188700
ggctattgtg aataatgctg caatgaacat ggaagtgcag agatctttta tgaagtggtg 188760
atttcatctt tttggggtat atgcccagaa ggtagattgc tgggtcatat ggtagttcta 188820
tttttaattt ctgtgggcac ctccgtactg ttttccatag tggtttgtac caatctacat 188880
tccttccaac agtgcacaag ggttctcttt ccaccacgct ttcatcaatg cttgttatct 188940
tttgtctttt tgataacagc tatgtaggat attctgaaaa tatatttaaa atattcttat 189000
ttttcctagt cagtatttcc tgacttttct cctctaggtt gcatgtattg ctttagtaat 189060
aaacaaaagc attaatgaaa acttcgtttt gaaaaaattg gatctgggat agaatttacc 189120
attttgttgt gggtttattt tctgatatgg atttatttta ctatttcact tgtaatttta 189180
gtttttctca acttaggtaa gaagacgaat gagaaggtga gagacagcta gtgcgtgctc 189240
atgggatatg acatttgaag ggaaaaggat ttgatagaat ctcaggcatt tgataaagag 189300
ttatttggca cttgcagtaa gctaagcact gttctggttt ggtgcacact gtgatgaata 189360
agagagtttc tcagccttgt ggggattggg gagataggaa ctaactaaat attttcaaaa 189420
taaaaacagg atttcaggtt tgataggctt gataataagc atgcagcata aagtaaaaag 189480
gagtgatgag gtcaaatggg gcctatttta tataatatgg tcagggaaac catttgaaag 189540
aggtgatatt tgaacagaga cttgaatgct gtggcagagc agaagtttgt gaagagctgg 189600
ggaatgagat tttttaaaat tatcctgctg ttggacagaa agtacagcaa gagatgatgt 189660
ggcttggatg agtgtggtgc cagtggagat gagaagggct tggatttgaa ttctgtcttg 189720
aaggtagcct tggaggtaga ttgtgcaaat gaaaagaaga gagcaatcgg gtaactgctt 189780
gtttcatttt gttttggttt ggttgggtct gggttgagta ctgggcatat gttggtacca 189840
tttatctgag atgagaaaga acagaagagg agtttttctg tttgttttgt ttggggtggg 189900
atgggtgtgg gaatgagaag tcaagaagaa tttgttggtt tgtatacttg atttgaggca 189960
tttactagat acctgagttg atatgttata tgtatgaggc tggagtttgg catataaatt 190020
tgggaatcat gtgctgtaga tggtgtgtaa atccatggga ctgcagaaga tcacccagag 190080
aatgtgggaa ggcagagaag agagaaccaa ggactaagaa tgtgctcagg aggttgagga 190140
gcactgatca gtagaggtgg aggaagtcgg gggaagtgtt ttagtgaagc atgtttccag 190200
aaggaggagt gatcagctgt gtaagtgttg ctgagaaatt gagttagatg aagactgaag 190260
attgacttac tggatttgtc aatagggaac tggtggttgt tctgatgagc agtttcagtg 190320
gagaggtgga cacacaagac tgatttgagt gggttttaaa cagaatgaga gccaaggaag 190380
tgagataaag tacttattgc attttagggg aggtttgcta taaagggaag cagaaatgga 190440
gctagagctt ggcaggggtt gggaagatgt agaatcaagg gagagttttt attttgtatt 190500
tttaatacaa aatacatgcg tatatttatg ctattgagtc tgatccatta tagaggaaaa 190560
aagctttgta gaagggaaaa ggataattta caggaccaaa gtatgacaga aaggctagga 190620
tctagcacac aagcagaagc atcaggctta ctggggagtg aggacagctg atctattgta 190680
acaggtggga aaactaatat gttgatacag aacaggtagt ttgatagata tgatggtgta 190740
atgaaacagc ggttccttct tcttgtttct gttttctcag tgaagtcaga aggcaggtaa 190800
tttgctgaga cccttaatgg ggaaggcctt attagagcaa tataaaatat tttattttaa 190860
aaatactact ttataaatgt attcttcact tagctgttgc tttttggttg ttattgtggt 190920
tagcaatata tgtctgaata tgttttcttt aaacaggtag agttgagttt ccctgggcca 190980
catagggcat aagtcaggag ccctcaccac cttttttgta tatggggaat ttgaaatcta 191040
gaaaccttgt gtttttttgc cttctccctc ttctggttct ctaatttttc atcaggcaca 191100
gtagagaagg agaaaggatg gtttcattta gttcagtgtg ctcattcagc tgttctgcag 191160
cctccaggtc ttgagagttg ttgagcataa tcccttgcac agtgaactgt cccaggcttg 191220
tgaaatccta gtggccactc taacacagat gactgagttg agatgttctt tttgttcagg 191280
ttttaaagtg aagtgccacc ctttagcagg tagagattat atacatcatt ctgttttctt 191340
tgaggactcc aagtgcccct cttagctgta agtagtagag accaacgtta attcatctct 191400
caatcttgta ttccattttc cagttcattt ccctgaacca tattttcctc atttgtggag 191460
taatacttcc cactcatggg ttcttcatca ggcagcttat gatgcatgtg aaaattgttc 191520
tggaaaagcc taaacagtac aaaaagagaa atcctattgt catggtgata attttgttat 191580
tctctagaag acattgacag ttccctagga ccacccctta aactcttgtt tttcatctta 191640
cagaggcatc catttcttgt tttatttctt ctgttagggg cttagtgtag tgacttgagt 191700
actgtttagg gttttgatat atggagttca aatttgcata ctacaaactg ggggtgaaag 191760
aaaagaagct gaatatattg cctgttttca aatagtttat tattcttcta actttttaaa 191820
accacaagct tccctcttat cacattttta caaatggata tatgaaagct ggtagaaaaa 191880
gcttgaattt aagatgataa aatgaacctt gttcttatgt ttttctttgc taattttcaa 191940
ttctgctgga gattagcatc ttctattagc aaggtgtaaa aattgagtgc agattgcagc 192000
ttttgggaga gtgttcaaaa atatttttac tttttttata attaaaaaaa acttcttact 192060
atttggaaaa gaaaaaatgt ctaatgacgt catctactac tgtggtattt cttcttaatc 192120
ctacctctag gggcaaatat ttcccatcac aataatagat ttttagtcct ggaagaaacc 192180
ttagggttca tctttttaaa ccattttcat gtaacttgtg agaaaatgaa gactcagaga 192240
agaagatttg tctttctgtc agttcatggc agagtccggg ttcaagttga ggtctctgga 192300
cagtcagtgt gtagggtttt ccttcctgaa ccatcctgcc ttttaaagtt tacagttgtg 192360
ttctcagaaa gagaagagtg aagttaccca gccacagtag aggaggagga tgggcagatg 192420
agttctgtgt acactttgtg catttaattt gcctcagtct tctcaatggt tttaactcat 192480
taccacaaaa tatagaaaac aaacaggttc tttgagttgt gctctctttg aaagctcttt 192540
aatagtaatt ctgaattatt tggtcttttt agtgtcatcc tagttacttc tgataaggat 192600
ggtcagagga ccaaatacag ttttgtttct actaatacga gtgtacatag gaatttttcc 192660
agctaactgt gacatagtat ttatatgcca tgggttaataaccactttcat gtgggttctg 192720
agatacatag gaaggttgaa aagactttag agacagctac catggtttta tttctcttct 192780
tctttttgag ggctgggaaa tctcaggtta tttaatttta gagtattttt aattaaaata 192840
ttttatgttt aacctctatt gtaaaataca ataaaaaatt taaaaaccac acagaaatgt 192900
atagtttagc gaattaagta agataccctg tagctcttac cactagctca agaaatgaaa 192960
cattgccagc cactctggaa aaccctttta tgttccccat gaaactcaca gatctctctc 193020
tacctcaaca agtaaccact ctcctgactt ttataatgcc cacttcttta tgtttcctta 193080
tggctttttc ccctgcttgt gcttagataa taaagttaaa tcttgctcat tttaaaaagc 193140
ctggtacgta ttttaaatct ttcttaatct acaggctccc ctccatttct ttctattcct 193200
tacagtttac ctgttgaaga acctgggcct tttgacctgt agcctttccg tagtctggat 193260
tttggtgatt gcatattcaa aggtgacaag atcatggttc tctgaggatt ggcttcctca 193320
tgggattggc ttccttatgg catagggaag ttactggtct tcttgatgtg gttttgggcc 193380
ctgtggtggc tttaagtcat ttggtggcac tgttctgctt tcctggttgc aatgctaatg 193440
gatacaggcc catatatagg cattttttgt ttgtctacct ttttctttaa aaaaaaattt 193500
gcacaaattt gtggggtgca tgagaaattt agttacatgt gtgtaatgca tagtaaacaa 193560
gtcagagtgt ttagggtgtc caccacccac gtacagcaca tgtttgttaa ctgtagcctt 193620
cctactctgc tatcaaatat tgaatttatt ccttctgtgt tcttaccctt taagccacct 193680
ttcttcatca ttccttctcc ccagtcaccc ttcccagcct ctgttatctg tctttcttta 193740
ctctctactt cttttttgta gcccaggcta gagtgcagtg gtgtgatctc ggctcacggc 193800
aacctttacc tcctgggctc aagcaattct cctgcctcag cctccctatg attacaggca 193860
tacgccacca ggcctggcta atttttgtat ttttagtaga gacagggttt caccatgttg 193920
gccaggctgg tctcaaactc ctgacctcaa gtgatctgcc cacctctgcc tcccaaagtg 193980
ctagatttca ggcgtgagcc ctcgcactgg cctctattct ctacttctgt gtgatcacat 194040
tttttaactc ccacatgtaa gtgaggatat gtgatatttg tctttttgtg cctgccttat 194100
ttcacttgag ataatgacct ccagttccat ccatattgct gcaaatgaca ttatttcttt 194160
ttgttctttt tatgtattta tttattttta ttatacttta agttctaggg tacatgtgca 194220
caacgtgcag gtttgttaca tatgtatacg tgtgccatgt tggtatgctg cacccgttaa 194280
ctggtcattt acattaggta tatctcctag tgctatccca cccccctccc cctaccccac 194340
aacaggcccc ggtgtgtggt gttccccacc ctgtgtccaa gtgttctcat tgttaaattc 194400
ccacctataa gtgagaacat gtggtgtttg gttttctgtc cttgagatag tttgctaaga 194460
atgatggttt gcagcttcat ccatgtccct acaaaggaca tgaactcatc cttttttatg 194520
gctgcatagt attccatggc ttttatgtgc cacatttttt aaatcccgtc tatcattgat 194580
ggacatttgg gttggttcca aggcaaatga cattatttca ttctttttat ggccaaatag 194640
tattccgtta ggtatatata ccacattttc tttatccatt gatggacact taggttgatt 194700
ctatgtcttt gctattgtaa ttagtgctac aataaatatg taagtgcagg tagcccttta 194760
atatgttgat ttcttttcct ttgggtagat acccagtagt gggattgctg gacaaatggt 194820
aatcttattt ttagtttttt gagcaatctc catactattt tccatagtgt ctgtactagt 194880
ttacattccc accaacagtg cgtaagcatt ccttcttctc tgcaccctga ccaacttctg 194940
tttttttttt tttttttttt tttttgagac agagtcttac tctgtcaccc aggctagagt 195000
gcaatggcat ggtatcagct cactgcaacc tccgcctcct gggttcaaac aattctcctg 195060
cctcagcctc ccgagtagct gggactacag gcttgtgcca ccacacctgg ctaatttttg 195120
catttttagt agagatggga tttcactatt ttggccagac tggtctcaaa ctcctgacct 195180
catgatcctc cccccttggc ctcccaaaat gctgggatta caggcgtgag ccaccgtgcc 195240
tggcccgatt ttgttatttt atgtagaact acttttatat tgagagccat acttgtggcc 195300
tcaacttact tgcaggttac tctggaagcc aactcctgtg aaggcagggg actcaaatta 195360
tcatttttcc atctttaatt atgaaggtca gctttcattt gctgtgaaaa caacacggat 195420
aatttgggac cataatacgt tctgaaatag ttcaagacaa gcatgttaaa aatgagggtt 195480
tttttttttc cccactgaac cttccctcaa gattttattg tcttcataat aaaagatgat 195540
gcttagaact agttaatttg gctctttctc ttcttttctc ctcccggttc aaaatgcttg 195600
cttctcttaa taggcagcat tctcttagat ttgcaactgg gctcaaagca ctgaagcctc 195660
atcacagtct tctttttggc agtagccttt ttacagaatt ccagaaaatg ggtttagtat 195720
gcccaccata gccactctgc ttcctgtcat aatgctgctt accctgggca cacatagaat 195780
ccttgtagta gaggcaggag gcagagaaat tgtagacaga caggggccgg accctggtga 195840
aacctcacca tcaaaaacaa aaccaaaaaa acagcctgaa acccgcagcc caaagtgaga 195900
acttctgttc ctgtttgccc actctcttct ggttggttct ttctgattaa tgccttttta 195960
ccaattgaat gttgcctttt ccaaaactac ctatgggctg ccctgccccc atcctgtgcc 196020
tataaagacc ccagactcag ttgggagaga gaggagagag agacagacag ccagacagcc 196080
agcctgacat cggggtagag acaacctgac tttggggaag atgacctgcg cttcccatcc 196140
cctctccacc tccactttct gctgagagct gttttcattg ctcaataaaa ttttccatct 196200
tcaccatcct tcagctatcc atgtgacctc attcttcttg gatgctggac aagagcttgg 196260
gacccatcaa ctgcaggtac ccagaaaggc tgccacacaa gccctttgcc ctttccagtg 196320
gagggcagct gccccatgtg atgaggcaag gggccaactg agctgttaac ccaccactgt 196380
ccgtggacag tggaactaaa ggagcactgt aacacccact ctggggcttc agggtcgtgg 196440
gcaccctcac ctggatgcca cctcaagggc cagctgagct gctaacacac gctgtctgcg 196500
gatggcagaa ctaaagaagc actgtatcac cccctctggg gctttggggt catgggcatc 196560
ctcacctggg tgcagctgtg ttccccttga ggcgacatgc ctggtctggc tgtgggccct 196620
gcgcggagct tgctcctgtg tcggcatctg gagcaactgg ccaggtcctg cacttgctcg 196680
ctcatgtgtt ccctccacaa ggggttgagt gtggcaggcc gagtagaggg ggcacccctg 196740
ctgggagtcc ggcaaagggg ccgacaaaaa tcctgcatta cttgctcttc ttgcactgtg 196800
tcattttgta gggttggtgc ttgccacacg cgttacagaa agtttgatgg tttttaggaa 196860
tgttcaccat gtttatagga gtgctattgg aacagaaaga aaaatgagtt tttttaagtg 196920
tcctaaaata ctgttagtct ctgcctcttg cccctttagt ggaacttttt gattatgcag 196980
aattacaact tgctgaagcc cagttgaggt agctttgaag gaagttcatt tcttcttttg 197040
cctctgttca gaggttgggg aattttgtga ctatgtaaat ttgctaagga gacaccattc 197100
cagattctta tctttcccag gactcaagtg gctcttgctt tgccactgtt tttgctgttg 197160
tttttgagac agggtcttac tctgtcgctc aggctggagt gcagtggtat gatcttgact 197220
cagtgtaacc tccgcctact gggtttaagt gattttccca cctcagcctc ccgagtagct 197280
gggagtacaa gcatgtgcca ccacacctgg atactttttt tttgtatttt ttggtagaga 197340
tggtgtttca ccatgttggc caggctggtc tcgaactcct gacctcaagt gatctgcccg 197400
ccttggcctc ccaaagtgct gggattacag gcatgaacca ccacctccag ccacttttgt 197460
attattttga ataacaaagg gcatttttgt tactccctgc aagggagtag cttttacatt 197520
aatacctttt ctttttgaaa atggactttg ataaaagatg taaaaagact ttgtaaaatt 197580
ttgtttgata accttttatg aggttaaaac aaagtttaat tctgattaca tggccaattc 197640
taaatttgag accaggtaca gtggcttata cctgtaatcc cagcactttg ggaggccaag 197700
gaggacagat ggcttgagtt taggagatcc agaacagtgt aggcaacatg gtgaaacccg 197760
tctctactta aaaaagaaaa aaaaagctga gagtggaggt gcgtgcctgt agtcccagct 197820
actcaggagg ctgagcctgg ggggcggagg ttgcagtgag ccaggatcat gaccctgcat 197880
tccagcctgt ctgaaacagt gagaccctgc ctcaaacaaa caaacaaaca aattcatttg 197940
gctgtgtctt ccacctgaaa tcagattggg atctcatagt actacagctc tcatagcatt 198000
caggtagatg gagctagaaa taagcccaag tatgcatttt ccttcaggac tttttaacac 198060
taagctagaa aatataagta tctcataaac cttatccctt taattaaatt tttagtagga 198120
accagaatgt aaatttcttc cttccctacc ccagaatgta gatgcatgtc ctcatttaat 198180
tttagtcatt cttggcgaat gtgataatta gggtaaagtg caatgagatt tgcaaattgg 198240
atggtgaatt cactaaatgt aacagctgat cagggcttct cttctcagtg tcactttatt 198300
aaggtcttcg gatatactga tatattctgg agtttcctcc ccacccccgc ctttttcttt 198360
tctctctttc tccccttgtt gaaagatact aacttctaca gtgttggtgt gagtagagta 198420
tcaggatatt gatcttaggg gaggtgaact aattatagat ttgttttttc attttatgag 198480
aggctattct tcataatagg aatatagaat atatgccata tacttgaggt tacttttgtt 198540
aatcccttta agaatggagc actttacctt gcttaacaag aaggaaataa ttgattcaga 198600
tgctgcatgc tgtgcaaggt gagttttgaa tgcagtgagg cgtatatgca gtggtctgac 198660
ttccgtaagc ctcactcatc tgactttcct ttggttctta atgttacact gaagactagc 198720
tgggcatggt ggcttatgcc tgtaatccca gcactgtggg aggctgaggc aggcggatca 198780
cctgaggtca ggagttcaag accagcctga ccaacatgga gaaaccctgt ctctactaaa 198840
aatacaaaat tagccgggcg tggtggcaca tgcctgtaat cccagctatg cgggaggctg 198900
aggcaggaga atcacttgaa tctgggagac cgaggttgcg gtcagctgag atcatgccat 198960
tgtgctgcag cctgggcaac aagagtgaaa ctccgtctaa aaaaaaaaaa aaaaaaaaag 199020
aaaaagttac actgaggact aaactctaac cttttttctc tcttgcccaa attcttatct 199080
aagggatcta gggtgtcaca ccctacaaac catataaatt tcatcaggtg ggttttattt 199140
aaccctatat aatatgcctt acttttcaac ctgactctgg cataatatca cataacagat 199200
aagaaaaatc aaaatatttt accctaaaat acgttttttt ttcttccata tcttaaaatg 199260
gccctgcaaa gccatctttt atagggggga gtatttgcat ctgagagtct atcacctttt 199320
aaaggtttgg ataggagaca tttgccattt atttgtctct aagggtggcc acctataaga 199380
cctcatctgc agaataacct tggtctccac aaccccccag acagttctat tgattccagg 199440
tcttagataa taactctttt gaccaattgc caatcagaaa atcttggaat ccagctatga 199500
cctgtgtgac cctcccccca cctgttctcc atatatccta cttttctgca ccaatccagt 199560
atatacctca catgtactga ctgatgcctt ttgtttccct aaaacatata caatcaaact 199620
gtaaccaagc gaccttgggc agatgttctc aggacctctt gagactgtgc ctggagccat 199680
ggtcactcat attgagcgca ttataaactt ctttaaatat tttagagttt tactcttttt 199740
gttgacaaca caaaaatacc attgaacctt aaatttcatg ttacagggaa gtgacatccc 199800
aaagactccc aggatggcta aacagtagaa aggagaactt ttattggtga tactggtttg 199860
taagctggga agaggaagtc tccagcatag actgaaggtg ctctctcttt gaagaggaaa 199920
aggacagact gggttttatg cctcaccagt tctgtattac acagtggagt catatatatt 199980
cagcaggttt gggggaaaag ctacatattt atgaggagag ctgagtacaa gcacaatgag 200040
taaacattta tgtaacatat accccatatt cactttgtgg tggggtttta gcattaaaat 200100
gagatggaat ttcactatct atatcaaaag gtgaacaaaa gcacacaaag actttgtgca 200160
gcccctataa actagctgaa actggtttct ggtctccagt agcttatcgg aaaagaatgt 200220
ttataaggcc catcatctgt ctaatcaagt ggcctgggtt gtaaatcagt tctgaaaatt 200280
tgcctgatag ctcctattgt taaggagtta aacaagaggg tggttactct tatagccata 200340
ggaatttaga aaaatgccat gccagccagg ccctgaaccc ttgacccaca ggtaactgtt 200400
tccttaactt tagggtctgt cttagttgat taaggggcat cattttgatc ttgcaggtca 200460
caggaggaaa aaagctcttc cactaccctc ttaggctcag tagctggggt ctatgaatca 200520
aaatgataaa ggacagatta acaggaataa aaacatacat ataactacat atggatatac 200580
agggtttaac aaatgaatgt gacttaagga cacagttaga atttggggct tatatcccat 200640
cttaacaaag ggaatgaaat acagagaaga gtcttgttag acaaagaaga aaaggtttgg 200700
ggcttcttga ggggggtaag ttgtgggaaa gggactaaaa agggtacagt aaataagggt 200760
catttgtaag gttttttaaa atgcaaacta gagtcttttc aggtgccaag agttgtcttc 200820
agagtagttc tcttcctgat actggagagg gagacacctt tacaaatgga actttatgtg 200880
ctgtttttag taagatgggg aaggacaggg agattttttt ctgtgtctgc tttttcttaa 200940
atgctttcag ctgaaaatag ttcttatgct atagtggcat atttttggat ggtatattct 201000
tgtccccttc catgaggaaa tgagttttga ggtgactgat aatactacac cagactttat 201060
aagggccgta tttggacctg caatgagttc tgtcctattt gaactcagaa cattaatttt 201120
cttttgctgt ttttcactta aaaatattct aatttcaagg cagttttata gtgtgtgcaa 201180
tatagtactg tagtactgca tgtatatatt ttatatgtag ttgcatgtat ttggggtaca 201240
tgagagcttt tctgttcttt gcttttgttt tcactggtga gtgccttcat aaaatgtcta 201300
gtggccactg gtctgtgaac ttatttgtgt ttattaatgt acaaataatg tcctaacctt 201360
ttatttaagt gttcatgtgt atttctgtat aatttgtata tttcagtgga gggtggtctt 201420
tccaacttca tgtgtgttct ttgaagcaaa aattcctctt ggtatctgac aggtatctaa 201480
gcttatattg tatgcacgat aggcacaaaa taaatattca ttaatgaatt tcaaaaatca 201540
tatataaaag gaagttgttg attttcatcg tttactccag aaatttttat ggattcatta 201600
catagcagtt agaaatggtg atggtagatt gagtagataa gttagaaatt gacatattga 201660
gaagtaatag gtgacaccca caaaagaaaa agaagagtga atatgtatgg cttat 201715
<210>2
<211>297207
<212>DNA
<213>Homo sapiens
<400>2
gtaattttat atttctaact caccctacct tatagttgtg gaaagattcc tctgaaacac 60
ttcccaaaat atcttttgct acaataaaga acaagtgtgg ctgggcactg tgactcatgc 120
atgtaatccc agcactttgg gaggccaggg agggacgatt gcttgagccc aggagagttt 180
gagaccagcc tgggcaaaat ggcgatattc ctgtctccac aaaaaattaa aaaattagcc 240
aggtgtggtg gcgtgcacct gtagtcccag ctactcggag gctgaggcag ggggattgcc 300
tgagcctggg agtccgaggc tgtcaatgag ctacgatggt gctactgcac tccagcctgg 360
aatgagaccc cagctctaaa aaatacaaaa aaaaacagaa agaaaaggaa agaggctggg 420
cgcggtggct catgcctgaa atcccagcac tttgggaggc cgagacgggc ggatcacgag 480
gtcaggagat cgagaccatc ctggctaaca cgatgaaacg ccgtctctac taaaaataca 540
aaaaaaatta gccgtgcgtg gtggcgggcg cctgtagtct cagctactcg ggaggcggag 600
gcaggagaat ggcgtgaacc cgggaggcag agcttgcagt gagccgagat cgcaccactg 660
cactccagcc tgggcgacag agtgagactc cgtacccccg ccaaaaaaaa aaaggaaaaa 720
gaacaagtgt gtctactcta cagcagaaat agaaactttg aaaactattt ttctaaatat 780
gtggtagaag ctttcacttg gcattatcag tacatctaat tttagctatg ccagtctgca 840
tggaatggta tctcattttg ttttttaaaa aatacttttt tcaccagcaa aggagacaaa 900
ataaaatcat gtaaaaaaac tcaaaccaaa agaaaacatt taaaaacaat aaaaacagat 960
ttaaaaaata gaaaataaat agtaaaataa tcaatttata tcaaacaact tttccccagc 1020
tttttagttg ctacagaaca tttctgtctg ggcgcggtgg cttacgcatg taatcccagc 1080
actttgggag gccaaggcgg gtggatcacc tgaagtcagg agttccagac cagcctgacc 1140
aacatggaga aaccccgtct ctactaaaaa tacaaaatta gccaggcatg gtggtgcatg 1200
cctgtaatcc cagctactcg ggaggctgtg gcaggagaat cacttgaacc cgggaggcta 1260
aggttgcggt gagccgagat cgcaccattg cactccagcc tcggcaacaa gagtgaaact 1320
ctgtctcaaa aaacaaaaaa caaaacaaac aaacaaaaaa gaacatttcc attgtaaagt 1380
ttcctcaact cctttcatcc tatgtcctgg ttccagattc caggcaatca ctgatcttct 1440
ctcattatag ttttgccttt cattgtggct ttactttgca tttccctgat agtcaatgac 1500
cttgaggatt ttttcatagg catattgacc gctgcctata tcttctttag tgaagcatct 1560
attctttacc agatttttat tgttgtctta gaattctaag agtttcttat atgttcttat 1620
ataagtcctt tgtttgcttt atgtattgca aaaacatctt cccagttagc agcctgcctt 1680
attttcttaa tggtatcttt tgaagagaag atatttttag cgaggtgcag tggcttaccc 1740
ctgttgtccc agttactctg gaggctcggg tgggaggatc acttgaggcc aggagtttga 1800
gaccagcttg ggcaacatag tgagaccctg tgtgtatata taatatatat aaatatatgt 1860
atatatatat atatatatat atatatatat attttttttt ttttgagatg gagtttctct 1920
cttgttgcct aggctggagt gcaatggcgc catctcggct cactgcaact tccgcctcct 1980
ggattcaagt gattctcctg ccttggcctc ctgagttgct gggattacag gcgcccacca 2040
ccacgcccag ctaatttttg ttgtttttag tagagacggg gttttgccat gttggccagg 2100
gtggtctcca tcgccgtgtt ggccagggtg gtcttgaact cctgacctca ggtgatccac 2160
ccgcctcggt ctcccaaagt gctgggatta caggtgtgag ccactatgcc cggcctaaaa 2220
aatatgtatt tttaaaaatt agccaggtgt ggcggtgtgc acctgtagtt ccagctactc 2280
aggaatgctg agctgcacct agctcttgag cccaggagtt tgaggctgca gtgagctatg 2340
attgcaccac tgcattccag cctgggtgac agagcaagac catctctatt tacaaacaaa 2400
caaacaacaa aaaacatata aaggagagaa gacattttta aaattaaaaa acttcaattt 2460
gtcaatcttt tccaaaaaga tttcccattt ccttgtagtt tcatagtttt agtttttgtt 2520
taaatctgta gtactattta cttatttttt gagacagtct tgctctgtca cccaggctgg 2580
agtacaacct aggctcaaga gatccacctc agcctctcca gtaactggga cctcagacat 2640
gcgccaccac acctggctaa ttttttatag aggtggggtc acattatgtt gcctaggctg 2700
gctcaagcaa tcctcccacc ttagcctccc aaagtactgg gattataggt gtgagtcact 2760
gcgcctggct gtagtccttt ttgtgttaac tttttttatt atttattttc caataattta 2820
ttatttggag atggagtctc gttctgttgc ccaggctgga gtgcaatgga gtgatcttgg 2880
ctcactgtaa tctctgcctc ctgggtttaa gcgattttcc tgcctcagac ttcccacata 2940
gctgagatta caggtgtgcg ccaccacgcc tggctacttt ttttttgaga tggagtctcg 3000
ctctgtcgcc caggctggag tcagtggcac aatctcggct cactgcaacc tccgcctccc 3060
aggttcaagt gattctcctg cctcagcctc ccgagtagct gggactatag gtgcgtacca 3120
ccatacctgg ctaatttttt ttgtattttt ggtagcgacg gggtttcacc atgttggcca 3180
ggctggtctc aaactcctga cctcaggtga ttcacccgcc ttggcctccc aaagtgctag 3240
gattatagac gtgagccact aagcctggcc ctttttgtgt taattttata tatactatga 3300
agtaagggtt aaggttaaat tgtttaccca aaggacatcc agttatttat ttatttattt 3360
actgtgacag agtctcactc tgttgcccag gatggagtgc agtggcacaa tctcagctca 3420
ctgcaacctc cacctccctg gttcaagcga ttctcctgcc tcagcctctc gagtcgctgg 3480
gattacaagt gcccccacca cacctggcta atttttgtat tttttagtag agatggggtt 3540
ttgccatgtt ggccaggctg gtctcaaact cctgacttca ggtgatctgc ccgcatcggc 3600
ctcccaaagt gctgggatta caggcgtgag ccaccgcgcc cagcccatcc agttattctt 3660
gaatgatttg ttgaaagact atttttcatt cattgaatta ccatgacact tttgtcaaaa 3720
acaactgaaa ccaatgtgtg gatctatttc tagactctca attctatttc ctttatctac 3780
atgcctttcc ttaggggaat accacactgt cttgattact acagttttct ataagctttg 3840
ctatcaggat ggataacttt gtcctttttt ttcaaaatta acttagctat tcaggattct 3900
tttcatgtcc atgtacattt tatttatttt tgagacaggg tcttgctctg tcgcccaggc 3960
tagggtgcag tggtacgatc atggctcact gtagccttga cctcctgggc tcaagcgatt 4020
ctcccacctc agcctcccaa gtagatggga ctacaagcac acacgaccac acctggctaa 4080
tttttgtatt ttttgtagag atggggtttc accatgttgc ccaggctggt ctgaaactcc 4140
tggactcaag caattcaacc accttagcct cccagtgttg agattacagg catgagtcaa 4200
tgtgcccagt tgattttatt tttgagacag ggtctcggtc tgtcgtccag gctagagtgc 4260
agtggtgtga tcatagctca ctttaaccct gaattcctgg gctcaaggga tcctcctgcc 4320
tcagcctccc aattagctag gactacaggt ttgcaccacc aggcctggct aaattttatt 4380
tttttgtagt gatggcagtg ggggtctcgc tatgttgccc aggctggtct caaattcctg 4440
gcttcaagtg atcctcccac cttggcctct caaaatgcta ggattaaagg catgagccac 4500
tgagcctggc atatcttatt acattacagt atcataggac ctcagttacg atcccaagac 4560
aagtgagaaa taaatatttt tcctcctgtc catcaccttc caatccataa atgtgagcaa 4620
acatcctctt agatctttaa atttttctct tagcaatgct ttgaagtttt cagtgtacag 4680
gtttcacaca tttttagaaa tttaccccta aattccttat attttgatgtg atcaaaaat 4740
tctattttaa ttttgttgct agtatataga caattgaatt gttaatgttg agttatatcc 4800
tgcaccttgt tcattgttat tagtggtagg atttttagta ttctctacgt atgtgatcat 4860
gtagtccgtg aacagtttta cttcatcctt tccaatgttt atgccttact acttgaagag 4920
aagtggtatc ttggtgcaga tctaaggggg aaagtgttca atattttatc attaaatgtt 4980
acctgtaggc cgggcgtggt ggctgacgcc tgtaatcccg gcactttggg aggctgaggt 5040
ggggtggatc acctgaggtc aggagttcga gcccagccag gccaacatgg tgaaacccca 5100
tctccactaa aaatacaaaa attagccggg cgtggtggca gatgcttata atcccggcga 5160
ctcgggaggc tgaggcagga gaatcgcttg aacctgggag gtggaggttg cagtgagcca 5220
atattgtgcc actgcactcc agcctgggcg ataaaatgag actccatctc aaaaaaagga 5280
aagaaaaaaa gttacctgta gatgttttgt aaattccctt tattacgttg agaaagttac 5340
cttgtactct ttaagctttt atcatgaact ttctaaaata cttactgtat ctattgaaat 5400
aatccataat gtgttttatt tctgttacca taataacaat aattgatttt ttcttttctt 5460
tttttttttt ctgagactaa gtcttgctct gtcgcctagg ctggagtaca gtggcgtgat 5520
ctcagctcac tgcaacctcc acctcccggg ttcaagcgat tctcctgcct cagcctcctg 5580
agtacctggg atttctggtg tgtgccatca tgtccagcta gtttttgaat ttttagtaga 5640
gacagggttt taccatgttg gccaggctgg tctcaaactc ctgacctcag gtgatccgct 5700
ggccttgact tcccaaagtg ctggacaggt gtgagccacc ggaccctgcc tgattttttt 5760
tttttttttt tttttgagat ggagtcttgc tctgtcatcc aggctggagt gcagtggcgc 5820
catcttggct cactgcaacc tccgcctccc aggttcaagc aattctctca cctcagcctc 5880
ccaagtagct gggattacag gcacacacca ccatgcccgg ctaatttttg ttattttttt 5940
agtagagaca gggtttcacc atgttggcca ggttggtctt gaactcctga ccttgtgatc 6000
cgcccacctc agcctcccaa agtgctgaga ctacaggcgt gagccaccgc acccagccct 6060
gattttcaaa taatatagca atcttgaatt cctgggaaaa ttccacttgg tcatcatgta 6120
aggttgttat aatatttgaa aatcaattat ataaaaaatt catttttata tattgctgga 6180
ttcaaattgc taatattttg ttaaagactt ttggaattgt ttttaagggc ttggagcttt 6240
cttttcttga aatatctttg atttggtata ggggtaatac tagcctcgtg aagtgagttg 6300
ggaagggctt ccctgttttc taaagtgtta tgtaagattg gtataattcc ttccttaaat 6360
gttgaataga attcattagt gaagtcatgt gggcctggaa gtttctttat gggaagggtt 6420
tcaattatga ctcactttct ttaacaggta aggaaatatt caaatcttct atttcttttc 6480
atgtctcttt tggcaatttg tgtctttcaa ggaacgtgtc catctcatct aagttgtcag 6540
atttactggt atatagttgt ctatagcaaa gagaaagaag acaaaggcca gacttccctc 6600
tgagtgacgg aaaattcctt atcacatact gaccttgtta ttagaagaca cttgatgcag 6660
tatttagggg taaggagtca taacatctgc aacttactct caaatgattt ggcaaaacac 6720
acacgtgcgc acgcacacat acacacataa gagagagaac ggacaggata tgaaacaaac 6780
aacattggtc aatacagggg gaagggtata tgggaattca ctggactatt tcaacaactt 6840
ttctgtagat taaacacttt tcaatatata aagttaaata atatattcat attccataac 6900
ctggcaattt aagggaataa tcagaaaatc acacacatac tctgcacaag aaagtttatc 6960
acagatcaga acttgaaaac accgtaaaac ccccacagag gattcaataa acaaacaata 7020
cgataccaaa gagccgccgc cgacaacgac aacaacaaca acaacaacaa caacaacaaa 7080
agacatactt ttttcaaggg tatccatgtg gaaaatgctc agtataaata ggctactgag 7140
gggaagagtt cccacactga gtacgtgacc ccttcctata tgcacaggaa gaagactgca 7200
gagaaacacc atgcaagcag tggctgtctc tgtggtggtg tcacaagtga aaatgactct 7260
tcctcacact cctcttccaa actttttttt tttttttttt taagacagag tctcactctg 7320
tcacccaggc tggagtgcag tgccacgatc tcagctcact gcaacctccg ctctctgcaa 7380
cctccgcctc ccaggttcaa gcgattctct tgcctcagcc tcctgagtag cctgccacca 7440
cacccggcta actttgtatt tttagtagag atggggtttc accatgttgg ccaggctggt 7500
cttgaactcc tgaccaggtg ggtgagtgat ccacccacct cagcctccca aagtgctggg 7560
attacaagca tgagccacca tgcctggcct cttccaaact tcttgaaaga tcctagaaac 7620
tatcactcca gagcaatagg aaaaagagct tacactggtt taagacctgg attaccccca 7680
aaaggaaggg aaggagattc tgcaggcagt agtgacaact ggtctcaaag tagcctggcg 7740
aagtggactg tgtaagactg tgagatctgg tctcatgccc cccaaaatag gcaaggttaa 7800
gagatcaagt aagacagtaa atgagaaaaa ggtaaagtta tatacacatg taaaagcaac 7860
tgtctatttg ttttacaagg atgttcatcg gcttggaagc tattactaca ggaaatccac 7920
atggtacaat gagatctaaa gtccaaagag ctgagtggag gtttgaatct gcttcctgga 7980
tgatcctggg caaactattc agcctttctg agcctatttc cacctctgaa aatctgggat 8040
gatgaaattt tttatcacta tcctccctgt tgaaagactg tgggctgggt gcagtggctc 8100
atgcctgtaa tcccagcact ttaggaggcc aaggcgggtg gatcacctga ggtcaggagt 8160
ttgagaccag cctggccaac atggcaaaac tccatctcta ctaaaaatac aaaaaattag 8220
tagagcgcag tggcgcgcac aggtaatccc agctactcca gaggctgagg caggagaatc 8280
gctggaaccc aggaggcggg ggttgcagtg agccaagatc gcacgactgc actgtagcct 8340
ggctgacaga gcgagactct gtctcaaaaa aaaacaaaaa caaaaaccct aaaaaacaac 8400
agggggacct gctgagtccc ctgagtccct ccatgtatca tgaatgagag gacgactgtc 8460
ccaactcata aatcctggag ttgctctgtt gctcttggcc tctgtgtggg gctgccacat 8520
cgtccctgag aacaatgctg actgtgcggg ctggaccact gtcctatgct ggaaaagtga 8580
ggaacaagca gattcctctt gaaatgcctg cctgagccaa ctggccacta tatgagtaga 8640
aaaattattt tgacagggat caagaagaca gagattgaac aagatatctg cctaaactgc 8700
agctggagat gtggcaagaa gatgtaagga ctcattgtag aggagagaaa tattttttct 8760
atccttttta ttttcatccc tgtaagggca ggaacaaatc tttctgggtt tggagtcagt 8820
gacaatactc acagcaagga gtccaacatc aaatactgag attcttgttt tacaaaagtg 8880
atctattctt aaggttgtga aattgagtct tctatttgac aaaaatcatt gttacccaga 8940
aacactttta aaatgttaat ataattcact gctttctgca ttatgaacaa tgaaagcaag 9000
aaatctggtc aaactgggat gttgagaggt aggctgagga ctggccactg tactatttca 9060
ctaaaactgg aaaaataata caaatgcaga ctccagaggt gaaattcagg cgatttattg 9120
taaacaggat aaccctagca ctccaccata ctggctgtgt cacatgctcc ctctttcaca 9180
tcaactggtc aaaaaagaag actttcttaa gaagcctcag gtaactaata acttagttcc 9240
acaaatctca tttactaagc actgcgtaaa ctgcagtatc tggggtagtc tgttattgct 9300
catggactgc agcttggacc catctaaatg aatccattag tatgcctgag tgatcaacag 9360
cagctctaga tccaacctgg aaattggaaa atgctgacgc ataccaaaca attgcaggtt 9420
catggaagac tacatgagat tgctctcaga tctcttcatg tgtcattctc ataaaaaaga 9480
cacttaaatt tcacccattt tttaaaaaag agctaaaagt tactcaatag cacataattt 9540
tccacataaa aggtaggtgg atttttattt actagtacag tggtggatcg gaacactcag 9600
gcctaatttc agatctgtct tgtatatact tgcagtttaa tgaaaagctg catagtctac 9660
aaatacataa aaccagaagg aagtgatgat ttcacacacc ttctgatgga taaagacagg 9720
cttcatataa ggaggttctt gaaggtaggg ataaaaagat ggttacagca ccacattgat 9780
ctgacagtag aaatggagcc ccgcttttga aagcagcccc atagttgtga ctatatcgtt 9840
cttgcccaaa attgagattt ggaaaatggc aaatgtaaaa acttaagatg tgaaaaggta 9900
tgactttaca gaagaaagtc ctagccaatc aaatcatatg gggaggtaaa atgtccttca 9960
aaaatgccat tctttacttt gcctgagtta gtggatgaca gggccactta ctgttactag 10020
tgcttgtata tggaaatata ttcacaattt ctcttgaagc aagttttaat aacagtaaat 10080
atttcaaaac agctgttaaa tctacagtaa caatttcacc taaaatagta tctcatgaaa 10140
aaaatatttt tttaaaagtc tactccagtc atcatctgtg tttatatgtt tatatttata 10200
tattcaaaaa ataacttaac atttaaggca agattagact ggtagcaaca agggatttta 10260
gagttttatg aagacctgct atataagaat gcaaggcttt actttttata tttccaaaaa 10320
ggtggctttg tattggctgc cttttaaatt cataaagggg caagatattt tggttcacat 10380
actgactgca atttcactgt aataaaaagt tatacaggtg gttacaaaaa gctgatgttg 10440
gaaaagcatg tcacacactc agtaggatct gaagttgacc ctatttgtac gtgttaaata 10500
aatatgttgt gttaaagcaa tatgttgatg aggaaaagta aaactcatgg aacactgcaa 10560
accaactatg aagaagtagg tcaatataat tcaatcagaa tgaaagaaac cacagtaatc 10620
ctgagaaatt cggtttagga caaaattgat gtaaggtaag tctgtatttc tagcattgta 10680
aataagtagg gttttctagc attttacgag ttggcataaa aaacatgttc aaattttata 10740
aaaagattaa aaccatgcct tatataacca gcatttcact aataaaagat gacattgctt 10800
tatcatctgc caaatgtaat gttttccact gatttacatg aaaccatcaa gtagaaagca 10860
aatttgagga tcaattttta aaaagttatt acctttctaa atgttgtgaa gccttctatg 10920
tcaagctagg aggcttttag aaaagagcta acttttaact ttcttcggac aagtgactat 10980
tttacagaat agggaaggaa gattctatag gaatatcatt cattttaagc attgttatat 11040
tggggaaatt tctctcagta atcaaaagaa aaattttaaa atatttaaga ggcatgtatt 11100
taaaaaatat tctacatcta taagattttg taatttactt tggatttata atatttctac 11160
ataatgacat ttgacaattt tttgtttgtt tctctaatag tgaatcagaa actattaaag 11220
tggccaagat gattttctta ggctctggaa gactcaggaa tgcatccact tgcactttcc 11280
catgcatggg gaatcttcag agttttgcag ccatgaagtt aatatgtggt ttcacaaggg 11340
gaaacagtgg cagaccacgc ttgaattcgg tcatgttctg aatcacttca ggctgcaaag 11400
aaaaaagttt ccttttagtg tatttaacct aaacatcaga ataaggacat caactttttt 11460
tggcggacac tgggagcata cctcagttct acttaatata ctgttttttt gttttggctt 11520
gaggccgctc tacctaataa cccatgtatc ttgatctgct gacctttctt ctggatcagt 11580
atgtctggag ggtcgtgaat tggtctgtac aaacctatgg taagtgtcct tagtcctttt 11640
ggttttcctc tgggcaaaca agggctggct caccaaggac ttagaggctt cgcatcaagg 11700
cttcagcaga gtagcagagg ctggtgtgcc cacccacaaa aagcctagtt acactgccag 11760
tttcctcctc ttgcaagcta atgtcaatcc ttctagatga ttaaaatacg caggtaacat 11820
gggagagact aagaagtctc tctccagtat tcagtgctgg gaacagatgt ggccagaaat 11880
gatccattgc aaaataacac aactgttcaa acaggaggca aaggcagaca agggtagagg 11940
ctgagtgact agatgttcag ggaagaacta aagtagaagc tccaacacct ggtgacctga 12000
aacttttagt tcatcacttt tagttcccaa tgaacaagtt tcttctggcc gggcacagtg 12060
tctcgtgcct gtaatcccag cactctggga ggccgacgca ggtggatcac ttgaggtcag 12120
tagtttgaga ccagcctggc caacatggtg aaaccccatc tccactaaaa atacaaaaac 12180
tagctgggca tggtggcgtg tgcctgtaat cccagctact caagaggctg aggcaggata 12240
attgcttgaa cccaagaagt ggaggttgta gtgagctgag ctgagatcgc accactgcac 12300
tccagcctgg gtgactgagt gagactccat ttcaaaaaca aaaaacaaaa aacaaaaaaa 12360
accaagctgc ttcttctaaa tagaaagtcc tctgtcagta caatctaaca taacgttata 12420
tttcttactt gtggcaaggc tggtgcttgt gacaaattta tgtcattttg acatgggaac 12480
tctccaacaa caggacctat aagaaaataa aaggtttaat actttgtact tattgatact 12540
atcacaaaag aacaaaaaaa aaaaactaaa ccagactcag ttaattaaaa acaccgcacc 12600
agccccgttc atgtacttct cagatcctct aaaggttgcc tgctacccaa ggccttaagt 12660
acttgtcctg ccttctatcc agagccaccc agtgatggct catgatattc ctgaggtcat 12720
aaaactttgg ccacagcagt tttagggccc aggtctaatg tgactttctg tccacccaga 12780
aggaccttgc taggtttcta aactgttagt aaacttacta gttttactaa actgcccaga 12840
aagcaaggga gttaatggcc acaggacaaa ctttggccag taaaagccag gaaacgaaag 12900
gactgggaag ttaacttgct tacccatact ccccacatgc agtggttcat gcagcctgtt 12960
tggagacacc ccaacagcaa gcagctgtgt tttcctgtga tgctgtggct agctcggcat 13020
tctatcacct ggtattgcct ctctccttca ctacctcatt tctcttttcc ccttatatct 13080
gcagacctta tctccagttt ccgttctcta cctcttttac tttctggtgt tctataaggc 13140
atgtagctat gacaaaggcc aaccatcaga tggctcaaca tgagcctaaa aaggcaacat 13200
ttgatactta caagaatcca tttccctggc aagaacatgg acggatacct tatgtctcct 13260
tggagcatct actgccaaca tttcctaggc acaaagaaga gcagggtcac ccttttgctc 13320
cactaaagaa cttacaatga ggtgttctct gaagactatg aagaatcagc tatcacacag 13380
tcagatatag aaaagatgct tccctttctg gccgggtgca gtggctcatg cctgtaatcc 13440
cagcactttg ggaggccgag gcaggtggat cacttgaggt caggagttcg agaccagcct 13500
ggccaacata gtgaaaccct gtctttacca aaaatacaac aattagccaa atgtggtggc 13560
acatgcttgt aatcccagat acttgggagg ctgaggtggg aggattgcct gacacgagga 13620
agcaagtttg ccatgtgcca tgagccgaga tcacgccact gcactccagc ctgggtgaca 13680
gagcaagact ctgtctcaaa aaaaaaaaaa aaaaaaaaaa aaaaaagaaa aagaaaaaga 13740
aaagatactt ccctttcttt cccctcactt acattatttt atttaagctc tagaaagatt 13800
tagcaaggca cgcaagaatt attctttttt tttcctttcc ttttcttact ctaaggaaag 13860
aattatcctt aacccaattt tacagatgag aaaactgaat ccaaggagat taaaaaactg 13920
ctcaaactcc tacagctaat aagtgcctga gctaggatta aaaacctagc ctagtactca 13980
ctgggatggg ctgtgcaggg ctgcacttgg tgatctttag tgtctacaga gacaatggtc 14040
tgagcttagt cagggacaag cacgagtgcc tgctcctcta agaaccttct cagactctgc 14100
ttgtaaactg tggttcatct accaaaagag aaaggaaact aacaattact gagtcctgcg 14160
ttatatcagg cactctccta tgtcagctca cttaattctc tgtgaactaa gcatcatgat 14220
gattcttact ttagaggcat ggactctaag acacagcagc ttacatgaat tacatgggtc 14280
acacagttca taggtgatgg tgtcagaagc tagagtctgc ttgctctctc cattccacca 14340
tgctgcttgg agggattccc cttagttact accatgaatg ccagcagtgc taagttggca 14400
ggtggatcct gtggggaagt atgtcccaac ctttccctta acagcagact tctactgtct 14460
tttctaaagg ggtacaaaat tgtgacaaag ttcctcttat tccttagcca caagcaacgt 14520
ttcatatcct tagaggaaag aaatcatcag ccaaaccaaa tccactcaac agttatttac 14580
taataactgt tactagttac tagtaactgg gcagttacta ataagcctgg tcctatgtga 14640
ctgcaaggaa gccctgttga tgttgcacag caatcctgcc cgtggctccc cactctactc 14700
cctgccagca ggcaaaacag cccagattct cacaggcctg ctctgacctc tccagcctct 14760
ttccaggaac tcagcccagg tctgcctcta ttaacatgta gtacctcatc taagaacttc 14820
atcttactgg agtaaaaagt ttgcttccaa agatctcttc tccatctctt gtcacccaaa 14880
ccttcattac ccccttgtct acccacatca gcagacagca gaacaccttc tccagtttca 14940
gattgttccc gattttctga ccaactctgg acaatcctca tttttctaaa aacaaacaac 15000
tgcaccttct tgcaaactat taatatgttc taaggaacac tacagtcctg ggagatattt 15060
ataggtattc tgtgtaaaaa aggatgcatg gtgaaatcag tttagaaaat gttagtcaaa 15120
attaaataaa tgcaggctaa agtctttact gtgggacttc ttataatttc taaaatacca 15180
atacactctg attctctaag aatgcgaata gtctttccca aacttgtatg gtcatgaaac 15240
ccttttccta ggtaccccta ttaatatctt gcataccatg gaacacagct agggaaatgc 15300
tgttccagac ttagcttagg cctggttata aatactctct ctgttttttt tttttttttt 15360
tttttttttt ttgagatgga gtctcgctct gtcgcccagg ctggagtgca gtggtgctat 15420
cttggctcac tgcaacctcc accttctggg ttcaagtgat tctcctgcct cagcctcctg 15480
agtagctggg attacaggcg tgcaccacca cgcctggcta atttttgtat ttttagtaga 15540
gacggggttt caccatgttg gtcaggctgg tctcgaactc ctgacctcgt gatctgccca 15600
cctcggcctc ccaaagtgct gggattacag gtgtgaacca ccacgcccag cccctctctc 15660
ttttttttga aacagggtct agctatgttt ccaaggttgg tcttgaactc ctgggctcta 15720
gcgatcttcc tgtctcagcc tccccagcag ctgggactac aggctattat tgcaaaagaa 15780
caagagtaat ttagatgaga cacttctttt cctctataca atgcaaagga agccatgagg 15840
taaagaaaat cacttgtcca gccatccatt tttgtagcca aaatgggtca aatctcattg 15900
ctgaaaaagg tgctttcaaa atactggctt ctgctgctca gttctgaaac tatagtctcc 15960
ttttcttatt gttaaggtga tatcagctat attaatactt gtttgcatac agccttaata 16020
attttgcaga agagttgctg ggagaggtgt aagcaactca tggatgatac aaaatcacca 16080
ttttcctcaa agaaactatc agcacctttg ggtcaaagac tgttactttt tccttaccct 16140
ctcactcagc actaggagtg gtgaattaaa taaacattag aaaccctagt acaggagaaa 16200
atgtgatcaa gcctcattgt accactaact tcacaaagtc attctaatga gaactgtaag 16260
cactgactcc tatgctggta gctatagtac atccctagaa aaaaatactg tagagtatct 16320
ctctaaaagg atgtgggcac agggcaggac tgagtccccg tccaaacaac ataatgacag 16380
atacaaacca gaaatggcta gaacattaag ggacaattct aaggtactat ggtaatcaca 16440
gagtataatt ttgctcctta ttttgaaaaa taggcatatg agtattttac acaaactaga 16500
ggccccaata tattacaaaa aacatatgta acactttagg tgcaattcca ttgcagcaca 16560
aattttgagt ttttgcattt agcatactta ttacacattc atatacctac ctaatggaaa 16620
ttttgctatc cttcttagaa tttttatata ggggaagatt tagatggtgt gaacctcttt 16680
tttttttttt tttttttttt gagacagagt ctcgcactgt cgcccgggct ggtgtgcagt 16740
ggtgcgatct cagctcactg caacctccgc ctcccgggtt caagtgattc ttctgcctca 16800
gcctcctgag tagctgggat tacaggcacc cgccaccatg cccagctaat ttttttatta 16860
ttattatttt tagtagagac agggtttcac tatgttggcc aggctgatct tgaactcctg 16920
acctcgtgat tcacccacct tggcctccca aagtgctggg attacaggcg tgagccacca 16980
cgcccagccg gtgtgaatct cttaaagctt gctgggaaga ctttaatggt ggtgcctccc 17040
tttctttttg atagacttcc taagtgagcg caagcatgta aaacttataa tatctctgac 17100
cacaattcaa aggtgagtaa tcatttattt taaaaagcag ccacagtggc acctaaatgg 17160
gtgaagataa tttatagttg tcagagcttg gaaaataatc tgattctcaa gttcttttgt 17220
tttctttgtt cttccttccc accaaacaag ctcagcaata ttctttgcag agtagggtgg 17280
agaacagaga gaagaaactg aaagggtgcc taaaaaggta gatctggaag tcaggaaggg 17340
ctgtcacagt ccttggttta gagcatgggt caatcttttg gcttccttgg gccacactgg 17400
aagaagaaga attgtcatgg gccacacata aaatacacta acaatagctg ataagctaaa 17460
agaaaaaaaa tttgcaaaat atctcataat gtgggctgta catggcccgc aggctgcagg 17520
ttcagcaaac ttggtttaga gggagaggaa aacagtactt ggttcaataa aaactatctt 17580
tattctaaaa gtcattaagc gtttgtgtca aaaaactcaa atccacaaaa acaccttcat 17640
aaatccagcc tgaatggctt caaagcagca acacacaata tagcacatac aataggtgtt 17700
ttggtttttt tgaggcacag tttcgctctg tcacccagac aggagtgcag tggcatgatc 17760
tccactcact gcaacctctg cctcccaggt tcaagcgatt ctcatgcttc agcctcctga 17820
gtagctggga ttacaggcgt atgccactac gcccagctaa tttttgtatt tttagtataa 17880
atggggtttc accatgttgg ccaggctggt ctttaactcc tgacctcaag tgatccttcc 17940
accttggcct cccaaagtgc tgggattaca ggtgtaggcc gccataccca gccctataat 18000
actttcatat cagtcaaaat ctaaatatat gaatttgact taccttgtag aatttgatga 18060
tatcttcctt ggtaagtgtc tttaaatatg caacctcagt gttatctgaa aaagtaatca 18120
aacaatattt tactaacatt ttcatatgaa gcagcccaga acagtacact aaatgacaat 18180
atacttcact ccaagactct taagtatcca tatatatttt tttttgagac agagtttcac 18240
tctgtcaccc acgctggagt gcagtggctc gatctcggct cactgcaacc tctgcctcct 18300
gggttcaagt gattcacctg ccttaagcct cctgagtagt tgggattaca ggcacaagcc 18360
aacacgtctg ggtaattttt atattttcag cagagatggg gtttcgccat gttggctagg 18420
ctggtcttga actcctgacc tcaagtgatc tgtccacctc agcctcccaa agtgccggga 18480
ttataggcgt gagccatcgc gtctggccaa gtattcacaa tttttttttt tttttgagat 18540
ggagtcttgc tctctcaccc aggctgttgt gcagtggtgc gatctcagct cactgtaagc 18600
tctgcctccc gggttcatgc cattctcctg cctcagtctc ccaagtagct gggactacag 18660
gcgcccgcca ccatgcccag ctaatttttt ttttcttttt tatattctta gtagatacgg 18720
ggtttcaccg tgttagccag gatggtctcg atctcctaac tcgtgatccg cccgcctcgc 18780
actcccaaaa tgttgggatt acaggcgtga gccactgtgc ctagccaagc atccacattt 18840
ttacgtgacc atttgctttg atttcaaata aaagtaagtg gatgttggct gggcacagtg 18900
gctcatgcct ataatcccag cactttggga ggccaaggta ggtggatcac tcgaggccag 18960
aagtttgaga ccagcctggc caacatggtg aaaccccatc tctactaaaa atataaaaat 19020
tagccaggca tagtggagta tgcctgtaga cctagctact cgggagaggc tgagacatga 19080
gaatcacctg aacctgggaa gcagaggttg cagtgagcca agatcacatc actatactac 19140
agcctgggcg acagagcaaa actgtctcat aaaaaaaaaa aaaaaaaaaa aggccaggtg 19200
cagtggctca cacctgtaat cccagcattt tgggaggccg aggcaggggg atcacctaag 19260
gttgggagtt cgagaccacc ctgaccaaca tggagaaacc ccatctctac taaaaataca 19320
aagtggtggc acatgcctgt aatcccagct acttgggagg ctgaggcaga attgcttgaa 19380
cccgggaggt ggaggttgcg gtgagccgga gatcgtgcca ttgcactcca gcctgggcaa 19440
caagagcgaa actccatctt aaaaaaaaaa taataagtgg atgtagtgca atgggtaaga 19500
gccttggctc aggagccagg ccagcctgga ttcacatcct ggctctctgg ttatgagctt 19560
ttggatcttg gccagtcacc tcaacttttc taagcctcag tttcctcatc tggaaattgg 19620
ggataataac tacttaataa gggctgcagt gagaattagg aaaaagatac atgacaataa 19680
cactgctagg tacacggcaa atgctcaata aatgagagac agtattatca ttaatagtga 19740
atccaactgc tagataaatt ataagtaggc tggacgtggt ggctcacgcc tgtaatccca 19800
gcactttggg agcctgaggc aggagaatcg cttgagccta ggaattcaag gctacagtga 19860
gctatgatga cacactgtac tcaagcctgg ctgacagagt gagatactct ctgaaagaac 19920
aaaacaaaaa taaaaattat gggtaaggaa actgaggtcc aaaaaaggtg tcttgaccaa 19980
gatttttagc ttgttggtgt cagagtaaga acacaaccca catcctctga cttctagtac 20040
agcttagcac aatccgttgt taatctatgc tctagaagca aggtgaaagg aagctgattc 20100
aagacaagag acttcaagaa tcacagctct cccaaatctg aaaggagtga aaaatctttg 20160
gagatcccta caggcaaatg tttgaactcc tgtgcctcaa gggcagcatt tgcctcaaag 20220
gggttcaatc tggtattatc tcattttttt agtattatgg gaaatagata cttaggtatc 20280
tgtgatcaga atctcaggat acactgatga aacaaaaatg tgaaattgaa ttattaatac 20340
tttattgtga gatatgggta gatccaggtc taggtttgga gggaaaaaaa agagataagg 20400
tttctaacag aaaactgggg ttaaattaag taacagttct ttggaagtgc cagccatccc 20460
cccaccccaa ctccccaaat aggtaacgga atatcaccta caaaatgaat accattttgt 20520
agaatggcct taatcaaatg ttacttgaat gccataaatg ttggagccct aattttacct 20580
tacctctgtc aaaattatat tgctgggaga tgatttctcc ccagtattta gcacactcag 20640
cagatagctt ctttggtttg tctagtcgac gaattgctaa tgcctgaatg tgtttttgga 20700
aggcctcttc tgtcatgtcc tctatggact tttccatggt aattaagaaa gcttccactc 20760
tgctttctag gtagtgaggt ggcttttctg actggatgat gaatctcaag ccctgtatgc 20820
cattagctcg acgtggcccg ctgaagacga tatagcctga aacacagaca tcagccagtc 20880
atgaccgtgg catttgagac ctgggtcatt tttaatcctt caaaaactaa cctgtctgag 20940
caaataaaat ctagtttaag gttttagagt attcacctct taaagaaata tgagcactta 21000
agatggtttc agttcctaag gacagagcag aagagctctc tgaagaatta caaagttgga 21060
gatgactttt tcttcttctt aggaaaaaaa tcctattcat tattcaaact cccatcttgt 21120
cactcctcac tctctgtgag ttaatcctct tcaatctatg ctaaagttac tcccagaata 21180
taaaatgctg gactcatttt catcccttag tgacagtcct agctgtgttt cctttaacat 21240
ccaagttccg catatgaaca cggctctcct actgtatttc ttgttagata atccattcct 21300
gaaccaatta attcagaatg caactctcta gagatcaagc taactaaaag tcatacttcc 21360
atgttatctc atgaggacat gcactacctt ccttcaacac cttttcaaaa ccaagctctt 21420
ggcttatctc cttcagctgc cctgccctga tctaccacct ccaacagctg tggtgcctct 21480
gggtctcact tctgaggaag tttctatctt caacatagcc attcacagtt tgattttctt 21540
tgagatggag tctcgctctg tcacccaagc tggagtcaag tggcacgatc tccgctcact 21600
gcaaactctg cctcccgggt tcaagtgatt ctctgcctca gcctcctgag tagctgggat 21660
tacaggcatg caccaccacg cccagctaat ctttgtattt ttagtaaaga tggggtctca 21720
ccatgttggc cagtctggtc ttgaactcct gacctcaggt gatccacccg ccgcagcctc 21780
ccaaagtgct gggattatag gcgtgagcca ccgtgcccgg ccaatagttt gagctcttat 21840
aataacattt accatgctgt actaaaatta ttcctttaca ggcctgccat cctgatttga 21900
ctgtgagctc ctcgaaagga aggaccatcc agagcatcca ttttccatgt ccagcaccta 21960
gcacaatggg tatttaaaca atgatgggta cttggagaga ctcatccttt tttcactggt 22020
tcctttctgt cattactggg gcttctttga gcttctttcc tcattgatca aatctgccag 22080
tacctcaaga cttttaacag tatctaaaac tgggaacact tgctgtgcct tctgtatgtc 22140
ccaggcttgg gatataaaga cgaatgagag ataatacctg ccctcaagga actcaggctt 22200
tagtgaatct aagctcctct ggatggctca tttctcatct tgctacccat aagcttgtcc 22260
ttagtatcct tttcccccac ctaaaatgcc ctctttattg acatgtatgt aatatgcagt 22320
atcatctcat aaataattgc tttaatgtcc ataagatagt gattgcttat cttttagaag 22380
tggattctct gtaataaggt tttctcaagt ctctctaagt agaccatgcc tgatatacag 22440
tgttaggtga aaatgtggaa aatgttttac agggaaaata attcatcaaa gtctacagta 22500
ccctgctatt tccccacttt cctctcaccc aactgctcct tggtgcgcag ggtgttgaag 22560
caaggttccg agataatctg acagaagagc tccagaaaca tattctctga ggtgctttgc 22620
atgtctgttt ggtagtatat ctcgatgcca cagttattgt gaacttcatt tctctgctga 22680
taaacaaacc atcctcctag gaagtttcaa aaagacagca gcaagttcaa aaacttattt 22740
ggggtttaat aaagtcaatg ctatgtctgc ccacacttac agatgataat ttagacatta 22800
atagaaaagg gaggtagaga agcctggagt tgtatttcat tgatttcact tttctgatca 22860
tgtccaactt tgaccaacag tcaaacttac taattataaa ttggcagcac taagttccct 22920
ctcatcagaa ggaggggagc cagtgccatg gctcacattt atagtcccag ctacttggga 22980
ggctgaggcg ggaggatcac ttgaccccag gagtttgagg ctgcagtgag ctgtgagtat 23040
accactgcac tccagaccaa gacacagagt gagaccctgt ctcaaaaaaa aaaaaaaaaa 23100
aaaaagcaga gagagaggaa cgtggcctgt aagcctctct ccctcaccca cctcacatcc 23160
ttctagcctt tttgtacttt tttttttgag acaaagtctt gttctgtcac ccaggctgga 23220
gtgaagtggt gccatctcag ctcactgcaa cctccgccta ctgggttcaa gcaattctct 23280
tgcctcagcc tcctgagtag ctgggactat aggcatgtgc ccccacacct ggctaatttt 23340
tctatttttg gtagatacgg ggttttgcca tgttggccac gctggtcttg aattcctgac 23400
ctcaggtgat ccgcctgctt tggcctccca aaatgctggg attacagatg taggccacca 23460
cgcctggccc ttttcttttt ttttttttga gacggagtct ggctctgtca cccaggctgg 23520
agtgcagtgg tgcaatctcg gctcactgca acctccatct cctgggttca agcaactctc 23580
ctacctcagc ctcccaagta gccgggatta caggtgcctg ccactatgcc cagctaattt 23640
ttgtattttt agtagagacg gggtttcacc atgttggcca ggctggtctc aaactcctga 23700
cctcaggtga tccacccacc tcagcctccc aaagtgctgg gattacaggt gtgagccact 23760
gcacccggct gcctggccct tttcatatat attctaaaac atcttctcgg ttgcagtgag 23820
ctgatattgt gccactatac tccagcctgg gtgacagagt aagactccgt ctcaaaaaat 23880
aataacaaaa ataaagataa ataaaacatc tcctttataa tgcttgaaaa ttttcctttt 23940
cacttgagac agtctgtcac tctggcaccc agactggagt gcagtggcac aatcatggct 24000
cattgcagcc tcaacctcct gggctcaagc gatcatccca cctcagcctc ctaagtagct 24060
acaggtgtgc gccactatgc ccagctaatt ttttttattt taaatttttt gtagaaacaa 24120
gttctatttt acccagccta atctcaaatt cctggcttca agcaatcttt ttaccttggc 24180
ctcccaaagt gctgggatta taggaatgag ccaccaccca ggcctgaata ttttactttt 24240
aaatactaag tgcctttcca tggagagtta agtgaagaac tatttaaaga ttcacatctg 24300
atcagctggc taattcagca tgatttccta ccacttagca ttttgagaat gagatgtgaa 24360
ctgggctatt tttgaacatg gagaaatttg ggagggtact taggaggcaa aagagattaa 24420
gatcctagga aaggagacag aaagagggaa gtagagtcaa aagaagtaag aaggatgaat 24480
tgcactttga aatattgttt ctacactggc ctcactttga agtcaactgt tatggctctt 24540
gcatagaata tcctgagtct ttctctaatt gtagtgggat cagcacatcc tgagtcttgt 24600
cctggtttta gtgactgagg aataaactgg caaaagttgg aaataaacca ctaccagatc 24660
tgtgatgact ggtcatgcac aacagcagag ctgggctcaa cgtatctcta agcattcaac 24720
aaaggtatcc tgaagtaata atatgttcca gttgctttag aagctactca aatgaactag 24780
acacagactt tggctttaag gaatgtacag tctaacaaga agatggagat gcacttcaat 24840
cccaacacaa gagaagtgct aagtagcttg aatagggagg ttcagaaaaa ttgtgctgca 24900
agtccagagg tagatgtgat tatttctagc tgggagtttt agaaaaagtt tagtggaata 24960
agtagcttat aaaataggcc tggaaggaga agtaggatgt aggcactggc ccatgaagaa 25020
gaagggggaa aggagaacac cattttggga ggataaaata gctgggggga aaaggcagca 25080
cagatggttc atgcataggg aacaaccaaa caatcaagct tggccaaaaa aagcttctct 25140
tccttcattt aatatacctt ctattacaca gttttgagca atattgacaa taatgcttaa 25200
tgagttttta tcagaagaag ccactgttaa tgtaaaagta acccaacatt gtaaacccca 25260
atatataaat tcaataaaag tctttgttaa tcccttataa aaatatactt gtaatgatta 25320
catgttatct gcacctgtgg gtttcctaaa actatttctg ccaaagaatc ctatgggttt 25380
ccaattaaat attataattc cagtttccat ttttataaat gtattttatt tcatgactca 25440
tagtcaatac ctttgtgata tttttacttc ctaattcccc ttcattcaag tacccgtcaa 25500
ttccagtatc attctcttca aaattattgc ctatctccaa ttttacttat ttggccctcc 25560
taccaaaaat ttttttggca gaattaccta tctctaatca taattctgta aataggagag 25620
gttcccaagt aaatctttgg cgtaaaacta taccaaattc agcccacctg taatcaccca 25680
cactgttatg tcaaaatagt ccattcccaa agttacaaca tctctactta ctgtcaggga 25740
gctgaacttc tctataccga accagctgac ttggaaggag aggtttggta tgagcatgtt 25800
caatgagggt gtcttcaacc atctgcataa ttcctaatgc agcctatgga aaaagatatg 25860
tcccagcata ttacaaaaac ataaacatat cttgtttgtg aaataaactg gctttgttta 25920
tcataaaagt ggattctaaa accttaaata tatttgatcc tattcaaatg gataaaaatt 25980
gaatttgtca gtatggtcat tttgcaatag agtcaatatg gtagtggtga aagaaacaga 26040
cacccaaaca atcatcaaca ctgatgctga tgctggaaaa aaggcactgc caaagatggt 26100
ctcaagtgaa atgatcctga ctacagacac ctggggtcac agaaaagcaa gggaggcaaa 26160
cataggcttt attgtaggta aaatgacctt ggtaagaatc ttgcttctgc cacttattag 26220
catctgacct tgggcatatt gttctggagg gggtatagca gagtggtaga gaccaaggga 26280
tcttttcttt tttttttttg agacagggtt tcgttctgtt acccaggctg gagtgcagtg 26340
gcacaatcat aactcactgc agcctcaacc tcctaggctc aagagatcct cttgcctcag 26400
cctcctgagt agctaggact acaggcatgc actaccacac ctggctgttt ttcaattttt 26460
tgtagagtta tgttgcccag gctgctctcg aactcttggg ttcaagtaat cctcctgcct 26520
cagcctccaa agtactgaga tgataggcgt gagccactgc acccggccga gactgaggga 26580
tcttgagaca gactgccacg gaaattttgt agtgctttcc atgtgctaag tacttagcat 26640
gtactattta atccttacca cacatgacgt aggtgctact gtaactctaa ttttacagaa 26700
aagaaaacag ataagcacag agttaagaca ttttcaaaag attacagatc tagtatatag 26760
cagagccagg atttgaatcc aggctgtcta attctacagc acgtgctctt atctactata 26820
caattatgcc accctataca gagtagggat aattactccc atttcctcct atgattgcac 26880
aaggtagaaa acagcagagg aggcagccat gaaagttagg tcctcagtta atcaccatca 26940
ttctactttc cctgtgccct ctaccttcct ccacagagca agagggttga gtaaactcta 27000
atgtgttcct tatcacacca tgctggttaa caatcttgag aaagactaga gggaaaaaat 27060
atcttgcaat gatccacaaa agattgctaa aacctcaacc acccacctgc tttgttatgt 27120
ttccatggag aagggcttca atgtgcagcc gtgacaggag ctgaggtatg aaggccttaa 27180
ggcgaggaag ggttacatct gcaaaggtgt aagaagacat agtgagcgct acctatgagg 27240
gattttttgt ttgagataat ttgagactta cagaagactt gcaaaaatag tacacagagc 27300
tcctgtaaac acttcaccta gttttcccta atgttaacat ctttcataac catcaaaact 27360
aagaaattaa cattggtata atactattaa ctaaagtaca gactttattt ggatttttcc 27420
catttttatc ttttttttta ttccaggatt tgatccacaa ccctccatta catttagctg 27480
tcatatgtct ttagttccct ctaatctctg acagtttctc agtccttcca tatatttcat 27540
gatcttaaga cttttgaaga gtactgttca gttattttat agaagtccca catttgggtt 27600
tgtctggtgt tttctcacaa ttagatttca tgtctgtatt accacaaaga ataccacaaa 27660
ggtaatgcgc ctttctcact gcattctatc aggggactca tgaagttgat atgttgtagt 27720
actagtgatg ttaaaactga tcacttggtt aaggtggtgt ctgccagatg tcctcactgt 27780
aaatgtatta ttttattctt ttatttataa tttaataact catatgtgct ctatatgcaa 27840
tttggaaaag cccacttgcc catctacacc tatttttccc tttgtaatta ctaaatattt 27900
attttgggaa aagtaaatga gattatacaa atattttctt tttctttaaa tttgacccac 27960
taattttagc atccattggt agttattgcc tgtagcaatt attactatgg agttctaatg 28020
gcaatttttc tatttccctc tttcttttta catatattaa tttaatcctt ctgtaaggaa 28080
gagctgtccc ttctccatta ggacaccata gtaataactg ctacatgcaa gatctaccaa 28140
tggatgctaa aatcagtgat agacatttaa ggagaaacat gggaatgcaa ataacaagtg 28200
cttaccacag atcaggaatt ttacagatgt tatctttcac actataaagt agctactatt 28260
atccccactt taaagatagg aaaatgaagt taagaaaatt taagtttgtt gttcaaagtc 28320
attaagctag taaattgcag agctgggatt tgatccttgg tcaattccaa agtggatgtt 28380
cattctattc cactgtactg ccttaggggt cctcttcact aggctccctc ggcactctct 28440
gcagctttcc accacaatac ttaccccctt gtctctctct attaaaatga tctacttaca 28500
tttatgcttc accaccagac tggagctcct tgatcaaaga aaccccatct aattgatcat 28560
tttaaatttt tatttattta tttttgagac agagtcttgc tctgtcaccc aggctgaagt 28620
gcagtggtgc aacctccgcc tcctgggttc aagcgattct cctgccttag cctcttgagt 28680
agctaggaat gcaggcatgc accaccacgc ctggctaatt tttgtatttt ttgtagagat 28740
ggggttttgc catgtttaat tgatcatttt ttaatctcaa gcacctgcag tcttgggctg 28800
ggtgcatggc aggcagtcag taaatgctgc tggaccacac tgaaccctga agttcttgct 28860
accctggcac ctggatagct gaatgattta gagtcactga agaggcagcc tctcacactg 28920
agttgtttcc aaattaagcc attctggcat tttggattat tgtaggagta tatatatcaa 28980
aatggaataa ttatttactt gagcagaaaa tttaaataaa aaaagaactg actattctct 29040
ttacaaaatc cattttagga agccttcata tccctctgag atactataga gcttattctg 29100
cttaaaatta acatgccatc tgacctagat tctaacgaag ggtgaataaa accctcaaac 29160
ctttgctttt tgatcattat aaaagtaggc ttcgttttgg aaaacattta aaattaattg 29220
tgtggtattt ttaaaaggta taattcttgc tattaattgc atatgaccat aatcattttg 29280
caaagaaaag catattaact gtcctgataa aaaaatgtac ctgttctagt aagtgatttt 29340
tataatctag tctcctatcc aatgcctgag gcaatcaaca aaataatgac tttcagtact 29400
ttgctcgatt tgtgtcactg atgacatcaa aagcatagtg ttacgtaagt ataagaactt 29460
gtcacaaaag gttattaaaa cattcaacag aattcttttt tttttttttt ttttgagatg 29520
gaatctctgt tgccaggctg gagtgcagtg gcacgatctc ggctcactgc aacctctgcc 29580
tcctgggttc aagcgattct cctgcctcag cctaccaagt agctgggact acaggtgaac 29640
gccaccatgc ccagctaatt tttgtatttt ttgtagacgt gggtttcacc atgttggcca 29700
gaatggtctc gatctcttga ccttgtgatc cgcccgcctc ggcctcccaa agttgctgag 29760
attacaggcg tgagccacca tgcccagccc agaatttttt tttttttttt tttagacaag 29820
agtctcactc tgtcgcccag gctggagtgc agtggcatta tctcggctca ctgtaagctc 29880
cgcctcccag gttcacgaca ttctcctgcc tcagcctccc gagtagctgg gactacaggc 29940
acccgccacc acgcccggct aattttttgt atttttagta cagacgggga ttcaccatgg 30000
tctccatctc ctgacctcgt gatccgcctg cctcggcctc ctaaagtgct gggattacag 30060
gtgtgagcca cggcacccgg cccaaaattc tttttattgc attttaatgc ttatggctga 30120
gcacatagga agaaagatta attggcgagg taatgttgag aagtaaaaaa tctaaaacaa 30180
taataaaggg tgataccaat ctacattttt ttccagtaaa gaaatcctga aagtaatttc 30240
tttacattca ataggcattc attagataca cactgagggc ataatattat actagatact 30300
aagtaaaggg gtatggaagg caggtataat cactgctctt aaggaatttt ggattttcaa 30360
gccaaactcc tgaaaattag acttaagatt ctagggaaaa aaacaacaaa agatgactct 30420
ttaggctttc acattttcca atttttaact gaactgatct aaggatgcta aagcaggaaa 30480
aaaaaaaaaa cctgaacatt gtgtgttaag actttgaagt tcagctaact ttttcttaaa 30540
acttcaatat tggccggtcg cggtggctca tgcctgtaat cccagcatgc tgggaggcca 30600
aggcaggcgg atcaccggag gtcaggagtt caagaccagc ctggccaaca tggtgaaacc 30660
ctgtccctac taaaaataca aaaagtttgc caggtgtggt ggtatacacc tgtaatccca 30720
gctactcggg aggctgaggc aggaaaactg cttgaacctg ggaggtggag gttgcaatga 30780
gctgagattg caccactgca ctccagcctg ggcaacagag cagactccgt ctttaaaaaa 30840
aaaaacacca aataaaaatc cttcaatatt aacaaggtta gtacttgggt cagagcattt 30900
agtctgcaga aaaaaaaatt tttttttgga ggcagagaca gagtcttgct ctgtcatcca 30960
agctggagca cagtggcgta atcatggctc actgcagcct caacttcccg ggctcaagca 31020
atcctctcaa ccacagcctc ttgagtaagt gggaccacag gtgtaagcca ccatatccag 31080
ctaatagttt tattattttt tgtagagaca gagtctcact atattgccca gggtggtctc 31140
aaactcctgg gatcaagcga tcctcctttc tccgcctccc aaaagtacta ggattacagg 31200
tatgtgcaac ccagcccttc tgcagaaatc taaaagtagt aataattaac tggtgtaggt 31260
ccagattgaa aacagtaaat gtggctaacc tgcctgaatg caaaaaccac ttcaaagcca 31320
ccactgtttt tttacatttt atttttaatt gacaaataat aattgtagat attcatagag 31380
tatataatgt tattatatat acatataatg tatagtgatc acatcagggt actaagcata 31440
tccatcatct caagtttatt tatcatttgt ttgggttggg aatgttcaat atcttccttc 31500
tacctattta aaagtatatg ttactgttaa ctatagccat cctgcccacc actgttttta 31560
taaaacatat acacagccag ctttaataga actacaagtt atttaaaagt ataaacagtc 31620
aatatcagaa taaaccaaaa tgaatttagg aaaaaaatga agaaagcatt aagctttaag 31680
tagaaagtct caccatccag agcttctttt aactcatctt tagtccaggc cacttcagtc 31740
atcagcaagc ggaggtagta catggcatgc tggtgaggct gttcagcccg gaaattgtta 31800
agagatcgca tatactagtg aaagagacat gcgttcatta attttataaa tcttttaaaa 31860
atcatttcaa aagtccagtt ataatcaaat ttcaattctc tataaactgt attactagga 31920
tattcccttc tttccccttt ctgaagttat ataattaaga atggggttta ttcctctccg 31980
atctttacat aaaagtgcat gctttttctt tgtccaagct acatttgttc agcatctgac 32040
atggactata agtgccttca ctatgggata tctttagtcc ctcagaaagt tatccctagt 32100
tttcagggaa gtcttttgga atctttaggt tctacgctat aaagccatcc ccaaagtctc 32160
cataagcaag caacataaat aggtagccat ataaaatatg gaatctttat ttgaaaactg 32220
atggtagttt cttctagtat acaaggaaag gtcatagttt agcttcagaa gcagctgttc 32280
atcccatact tccattactg ggccaaacat gaattgaatg taagtggaag atatgtcaaa 32340
cccaaatagt gtgctctaat gtgacaaggt cttgaggtaa agcagacctc agtctgctga 32400
attgcgtgtt cttctgaact agaggttggg atggaacatt caaggaggcc ctgacagcca 32460
ctaagtgctt ctctttgaaa ttcagagctg caactctgaa ttgcagtgag ctcttggcac 32520
ccacagagaa gaggccaggt gtccaccaga gtctactagg atttctttct ggatggagta 32580
taggattttg caatgacaag gtgagatgaa tgccacaggg ttatgaattc ttcttttcct 32640
ccttgcttct tttatattat aaaaactatt atgaaaactt tttataatat agcaatatta 32700
tcttatgaat aaaaaagcag ggttcagaaa acttactgct tctttgataa tttcaaatct 32760
tttttcatca atctcaaagg tagccatttt ctcaataatc ttctttagta aaattggctg 32820
cttgtcattg taacctttca ctgaaagcta cagaaagaat tcagggtatt aaaagtctaa 32880
aaatatcaca aaacaacttc caaataaacg acttgatttt taaacatgta gaaaatccca 32940
aaacagacat gtaaaaaatg aaacaaattc aatttaatgt gtcataaata tatatagtgt 33000
aacacttagg aagataacat taaccatctt tttttttttt tttgagacag agtttcgctc 33060
ttgttgccca ggctggagtg caacggcacg atttcagttc actgcaacct cagcctccca 33120
ggttcaggca attctcctgt ctcagcctcc agagtagctg ggattacaga cgtgccccac 33180
tacgcctggt taatattttg tatttttagt agagatggcg tttcaccatg ttagtcaggc 33240
tggtcttgaa ctcctgacct caggtgatcc atccgcctcg gccttccaaa atgctgggat 33300
tacaggtgtg agccaccatg cccagcctta accatctttt ttaaaaaata tatttattta 33360
ttcgagacag ggtcttgcta gggtacccag gctagactca aactcctgag ctcaagtgaa 33420
cctcctgcct cagtctccca agtagctgga actatatgtg tgagccacag tgcctggcca 33480
ttaacaatct ctatacccaa aagcataatt ttacttttct gaaacaaaac caacagtaag 33540
taaactcacc tgacaccgaa ctatggtggc atatactata tcatcaaaac tcaaattctt 33600
tttataaaat taattgtaga atgtggctaa gttttttctt caaaaagaat atctaatgaa 33660
aggcagactt aaggtttcta caggtgaggt aagaagataa aaatcacagg gcatagtggc 33720
acgtacaagt cgtcccagct acttggaaag ctgagatgga aggcttgctt tagcccaaga 33780
gtttaagacc aacctgggca acatagttag actcctgacc tcaggtgatc cacctgcctc 33840
ggcctcccaa agtgctggga ttacaggcat gagccaccgt gccaggcagg atccacaatt 33900
ctaaccactt gcttatttgg taagttctgt tggtgttcca aaagctaatg gcaaaacaaa 33960
tgtgtaaaaa ggatacactg tatgtatcat caggaccttg gcttagcaca tttttttttt 34020
tctgagacag ggtctggctc tgccacccag gctggagtgc agtggtacaa tctcggctga 34080
ctgcaacttc tgctccgcag gctcaagcga tcctcccacc tcagcctctg gagtagctgg 34140
actacaggta cacaccacca tgtctggcta attttttgta ttttttgtag agacagggtc 34200
ttgccatgtt gcccaggagg ctggtctcga actcctgagc tcaaagcaat ctgcctgcct 34260
tggcctccca aagtgctggg attacaggcg tgagccaccg tgcccagcca acttagcaaa 34320
atttactttg tctctaaatg cctaacattt tgaaaagtgg ggaatggaga atttctatca 34380
tgatatttcc acatggtggc acgcaaagca gtaggggata tcactgcagc tagaatatca 34440
tgaaagtctc tacgctgtac aggatgatag gcaagaacct cagcaggagg gaaggccact 34500
ggccacggca agcacatcat ttttcctact gtaggaacta gactgacaat tagcagaaaa 34560
cccattttgt cataaataaa aattatgtgt tttgatggta atatgctact aaaaatcaac 34620
agaaaaaaga gaaaacagat gattactttt ctatttggag ctaattttga tctctgagct 34680
tcaaacttct gtggactctg ggaatggtta gattaataaa cattcttata cattaaacaa 34740
aagctaccag agaatttatt tatgtgaaga aaaaaaagtc actggaaaat aaataaaatt 34800
taaaattcct acaatctttt gtgtaagaat ttaaacaacg agtaccccaa aatgctcttg 34860
ttttcatttt tactaaaata tgcaatatat tttcaagaaa agtgaagatg aatgtgtgcc 34920
ataaaaggga tgttaactcg gaattgagat ataagcaagc ttggaaacac attccaaaga 34980
aaggatctag ttatttctta gatggattta ataaatgctt attttaaaac acacataaag 35040
taggtaatgg tgcatgctac aaaataagtt ttaaattaaa atgtcagatg tatctgaaca 35100
tagaaacttt atactactgt ctgcagaaat aaagggtgcc tatttataag taaaattcaa 35160
tcaccacaac aactatggat atcttctact tacggatgtt aatatactca attatataga 35220
aagaacaata tgctttcagg attaatatgt taaacatctc aggcagaaga gcatgcaaat 35280
aaacgtagca cacatacatg ttcacaagat gcactaaact ttattaaatc attcattagt 35340
ggaactaaca ctaatttgag ttctttgatc atcaaaaaga tgcaaggaaa aaaaccatta 35400
actttcttgt ttaaaaatgc gaagttaata gttttcccaa gctcaacata atgcttaaac 35460
ggggtggtgc cttttctttg gttttcattt tacttacaag tattgcattc attcctgatg 35520
caatgccata gctcaaacct gagaggcgtg ctgcatatgt atactctttt aaatcatcct 35580
tcaataacct gataaacagg tatgtcatgt tgcaatggag aggatcagca taaatgtagc 35640
gactaacaaa aagaaaaaca aaagaaatgc tttgatacct caagcagtgg catggtaatg 35700
aagaaacatg accgaagagt atggatatgc agcctcatga tctggatttc tagggaaata 35760
gtatatgcca accttaggtt ttacaaaagg aagacttcat tttcctttgc caaagaccaa 35820
gatttcaaat acagaatggg taagagctaa acctaaatca gggtccaatt ttgtttgagt 35880
agaaaaatat gacattctga tgaaagacgt ttaaaaaaaa agaaatcaag gaaaatttgc 35940
ataatatgaa tggtatgtag ttgaacactt agaataaaag ctaacttaaa aattaaatga 36000
gaaaatatga ttagccataa agattaaaaa aaaaagaaat taaactcaca ccctcaatca 36060
aaaaatgctt ccatcaaatc aatataaaaa atgttgatga gtggaaggct ctaaggcgtg 36120
ggtacttaca tacatcccat agatggtatt ttggagatca tagctcaagc ctgctagctc 36180
tgctgcatat gcatactcgt tgagtgagtc tttgaggagc tcaaggtaca aataggccat 36240
gttacagtgc aaggggtcca cataagcaaa tgggctggaa gaaaatgttg acagtaaaat 36300
agctgatttt attacttctc aatgtgatat ggccttttga gatggatcaa gatattgaac 36360
accggttaat atgatttcct gaggatatgg ttgaattctt gaggctattt cttatgagga 36420
aaaaaaagaa gatgtctgaa aaagaagcct gaagtgtgtg tgagtgtgtg tgtgtgtgtg 36480
tgcatgcgtg cgtgtgtgcg tgcgcactgg tagtagtgaa gaaggagaag gaactgccta 36540
ccccagatgg tgtggcagaa ctgacaagtt agaaatgcag ggtatgtgag cagtgggaac 36600
gggccaaaat gcatggctgt gcttaggcta atgggaggag cacggccgac agaacagata 36660
cagcatttgc atgtcttccc tctctctcca ataaagtcac atccttggtg gacaggaata 36720
aaggactggg aaatgtgtct gtggtaatta catgaactta gaaaaattga gacctgtgaa 36780
aatgttagct tatccctgag ttttccccaa aacagtaaag ctagagaact gtcagtaaaa 36840
taattgataa tttctatcat ataacagcag ccaaaactag ggaaaaataa aatagaaaaa 36900
tattttaatg agaaagaaca aagaagaaag aattatagag tacaagaact aaaccaccac 36960
agtgatcaac taaacttcat ttcccagata agaaacataa ttttcttcag gtcccctgag 37020
ttcgtggcag cagagctggg attcaaatgc aagttaaatg acctgctcct ttgactatac 37080
aaccttgctt accttttatc agaagaaagt aaaggtctca ctcattcaac aaatatgtat 37140
tatatgctta ctacagtgcc aggctcttct ctaggcactg gaaataaaga gtgaacaaat 37200
caggcaaatt ccctgacctc agagttgata atctagctag aggggacaca taataataaa 37260
tttaaatttg acatattagt tggtgacagt gctatggggt aaaattaagc agggtaaggg 37320
gaatagggaa tgatggggtg atggcagcag ggaagaaaga ctgctgcttt acataagatg 37380
gccagtcaag gccactctta caatgtgaca tctggactca gacctgaagg aggcaaggga 37440
gtgagccctg gggatatcta aggggtggag tggagaactt cccaggctga agaaatagct 37500
actgctaaag gcctgaggca ggtgcctgct tggcttgttt ggcagcaaat taaaagtttc 37560
actctacttg tagcacagta aatataacta atgtactgat aacaaagtca acattttgtt 37620
ttttaaagtt gagtcaaggt ctcactgtgt tgtccaggct ggtctcgaac tcctaggctc 37680
aagcgatctt cctgcctcgg cttcccaaaa tgttaggatt acaggtgtga gctatcgcac 37740
ccagccaaaa gccaacaatt ttatcaaagc tgataaaaaa gcaaaaatag ggataaaatg 37800
tgtagtcact tagagaatta aaatcaaaga caaagttcta cagctatcca aatattctcc 37860
aactggaaaa tctgaataaa tgttctgcca ttaaattttt taaggtatga tacatgatga 37920
cagatttcct tcttttggca aatagcagct caaaaactta aggcacaatt cacaaaaaca 37980
aaatcatcaa aacaaaagag aggatatcac agaggtcggc cccacaggca actatgcacc 38040
tttctcaggt ggaagtgaaa agccagaaaa tccaagtttt gggtctcaaa gcactatggc 38100
tcaggctcag aaaataattc caaggagtgt cctaaaatgc tacatccagc ctggcaaggt 38160
ggcatacgcc tgtagtccca gctactcagg aggctgaggt gggaggatca cttgagccca 38220
agaatttgag gctgcagtaa ctgcactcca gccttgggtg acagaggaag actctgtctc 38280
ctaaaaaaag agaacaacaa aaacagctgt atccacagca gctgtgctgg tgaagtggag 38340
aagaccctaa actaactttt aaaagagaat aggattcaac tgggatacct aaattctggt 38400
tcatgtgtta attttaaaaa tacgttattt tgtaaccaga tactatatgc ttaaatatgg 38460
atcacagggt tttaaaaacc atagctaact acacataata taatcttatt tctgaaaaga 38520
tggaaaggat atagacacac acatgaaaat ttcaattaaa atgtacaaga aacagaacag 38580
tggttacctc taggaagaca gaccagaggg agggatttgt gtgggaggca gacattttca 38640
gttctaggaa atgcgctagg cagtttagag aacacaaaaa tgtaaaatgg tctctcaaag 38700
ggtagtctaa agaaattcat catcatagag gccaaatgga aaagtggtga caagctgctt 38760
agagtgttaa aaatatcact ctcaaatatc aaaaaataaa aagcagcttt gtaatgccct 38820
ttaccaataa taagccacaa cctgacagcc catctttggt gtaaacaata aaaaatctct 38880
gcacagttct agataatctg tgaatctcat gcaataaaac cacaatattt ataatcacta 38940
taatctcaac atatttgttg atggaaaatc aaattccttt ccctctttcc aaagcattta 39000
aaccttatct acctctaatt tcatgtcaag ccaactgaat aaaaattaat gaaaaagctg 39060
attatcatat tgaacattac tgaacaaata aaccatgata ggcttatctt acaaaccact 39120
gaattttaga aatataaaat tatgaagtat aaactcacaa agtaaaatct gaaacaggtg 39180
attaacacac aaataagtaa aggaagacaa agatgaattg aaggtgcact gagaaattac 39240
tgcaatagca ttattcataa attttactaa acatcttctc cctttttatt agtaatgttt 39300
aagtaatcaa gccagaaata gaggaagcag tatctaaggg ttccagacaa aggacaaaac 39360
aagactcagg atcatttagt aactagtacc tcctcttcta gacaagttcc aggaatacaa 39420
agccaaagca ataaaaacac cccaaattat ttattctttc ttcaatgagt cttcttctgg 39480
gtaaatctac ggcctcctca gaatccaaaa ccctgaaggc taagaaggtc atcctaacaa 39540
tagctaacat aaatcatttc agctaaaggg catactcatt aatggggaaa gaaaaaaaaa 39600
aagaattaac aacactggtt tttaaaatac ctcttaacag caacagctct cctttttttt 39660
cttttctttt ttttttgaga cagagtctgg ctctgtcgcc caggctggag tgcagcggtg 39720
cgatctcagc tgactgcatc ctcctgcctc agcctcctga gtagctggga ctacaggcac 39780
ccaccaccat gcccggctaa ttttttgtat ttttagtaga gacggggttt caccgtgtta 39840
gccaggatgg tctcactctc ctgacctcat gatccgcccg cctcagcctc ccaaagtgct 39900
gggattacag gcgtgagcca ccgtgcccgg ccaacctctc cttttcttta cttttattta 39960
tttatttttt tcagcctctc cttttctagg tgaaataact ctaattcctt ttatttctag 40020
acatggatgt ttaagaagaa cagacaactc ctggaaacgt aattgttgat ttttataagc 40080
tttataagct agattcatct tacatacctg aaaaattcaa agttgagaca agccttcggc 40140
aaaaaaaact tatcatcttg tttgaaccaa agtttgctca tagctgtatc ctgtgatgga 40200
gaaacaattt ggttagggaa atagaggcgc attcagcaaa aatgttcaat caaatcactc 40260
ttcaagagga ggaggacaga agcaatagtt agagttttgt tctggagttg ctgtcccatg 40320
ggccttgaag catactcaag agtcagcagg taggaagctg gagacaaaga ctgcagactc 40380
ctgtagcaga gtgaaatctg ccttctattt cccctgtatt agggagatgg gggtacagtg 40440
aaggaagaga cagatcagag aagccaatac caactgtcct gtcttcgtct ttctggacac 40500
taggaggaag gcagaggcag aagtcccttg caagcgtatg gcagccagct tccagggtgg 40560
cccacaacaa tccttgcctg ctgagattca cagccttggg tggtcccctc ccacactaca 40620
ccagggttgg tctgccttac taacaaaata tgacagaatt aatggtatac ctctgagact 40680
gcattataaa agacaccgcg gcttgcatcc tgatcactca ctttccctcc tggatcactc 40740
actttggggg aagcctgctg ccatgtcaag caactctaca gagaggtcca tgtggtgaaa 40800
aattaaggct ggccaacaac cacataactg aacttggaag agaattctcc agccctggct 40860
gaaccttcag atgactgaaa ccttggccaa cagcttgact ataacctcac gacaagccct 40920
gagccagaac tacccaacta agtcactgct ggattcctga ccctcctcag aaactgtgtg 40980
agggccaggc gcggtggctt cactcctata atcccagcac tttgggatgc cgaggtgggt 41040
ggatcatgag gccgggagtt cgagaccatc ctggccaaca tggtgaaacc ccgtctctac 41100
taatcataca aaaatgagca gggtgtggtg gctcgtgcct gtagtcccag ctactcagga 41160
gactgaggca ggagaatagt ttgaaccagg actcgggagg cagaggttgc agtgagccga 41220
gatcatgcca ctgcactcca gcctgggcaa cagagcaaga ctccatctca aaaaacaaaa 41280
caaaacaaag aaactgtgtg tgagatacgg tgcagcagct cacacctgta atcccagcac 41340
tttgggaggc caaggcaggc agatgacttg aggccaggag ttcgagatca gcctggccaa 41400
aacagcaaaa ccctgtctct attaaaaata caaaaattag ttgggcatgg tggcacatgc 41460
ctataatccc agttacttag gaggctgagg catgagaata gcttgaacct gggaggcaga 41520
ggttgcagtg agctgagatt gcaccactgc attccagcct aggcaacgga gagagactct 41580
gtctcaaaaa aaaaaagaaa gaaagaaaaa aagaaactgt gtgtgagaaa ccaggcatga 41640
tggttcatgc ctataatccc agaaatttgg aaggctgagg caggatcgct tgagcctagg 41700
agtttgagat cggcctgggc aacatagtga gatcccacct ctacaaaaat attgaacaat 41760
tagctgggcg tggtggtggg tgcttgtagt cctacgtaca caagaggctg aggtgggtgg 41820
atcactttag cccaggaggc caaggctaca gtgagccatg actgcaccac ttcaccccag 41880
cctgggcaat ggaatgcgag tcttttaaaa aaaggaaaaa aaactgtgtg agataataat 41940
tgttgtgggt tgtttctttt tctttctttc tttctttctt tttttttttt tttttttgtg 42000
acagagtttc gctcttgtta cccaggctgg agtgcaatgg cgtgatctcg gctcaccaca 42060
atctccacct cctgggttca agcgattctc ctgcctcagc ctcctgagta gctgggataa 42120
ggcacgcgcc accacgccca gctaattttt tttagtagag acggggcttc accattttgg 42180
tcaggctggt ctcgaactcc tgacctcatg atccactcgc ctcagcctcc caaagtgctg 42240
ggattacagg cgtgagccac tgcatctggc ctaacttaaa tgttgtggtt ttaagctgct 42300
aaattttgga ataatttaat atgcaaccat ggataaccaa catggccaag gatgaacaaa 42360
taagctactt atcttgcctc tccacttcac tcatgctatg ctaagaatgg ctttagtcac 42420
ttgaaactac cttgaaactc tgggaagaga tataaatggt ttaatgttct cgttagtttg 42480
ctctgggaag gcctatttaa aacaaacagc aaaagtcgga tatggtagct cacacctata 42540
attccagcta cttgagaggc tgaaggggga ggatcacttg aggccaggag ttctagacca 42600
gcccaggcaa catagcaaga tcccatctct acaaaaaatt aaaaacaaac tagctgggtg 42660
tggtgtgcgt ctgtaatcca gctacttagg aggctgaggc cagaagatgg tttgaaccca 42720
ggagtttgag gctgcagtga gctatgatcc caccactgca ctccagcctg ggcaacagag 42780
agaccctgtc tccataaata aataaataaa taagataaga taagataaac aatacataaa 42840
aaacagctga ggtaaatgaa ctttctcttt gcataagcta atataaaact ctttaaaatg 42900
tcatttttaa agaaaaacgg gtccggcgtg gtggctcatg cctctaatcc cagcactttg 42960
ggaggccgag gcaggtggct cccctgaggt caagagttcg aggccagcct ggctaacatg 43020
gtgaaaccct gtctctacta aaaatacaaa aaatcagctg gatgtggtgg cgggtgcctg 43080
taatcccagc tacttgggag gctaaggcag gagaatcact tgaacttggg aggcggaagt 43140
tacagtgagc cgagatcatg ccattatact ccagcctggg caacaagaac aagactctgt 43200
ctcaaaaaaa gaaaagaaaa taaagaaaaa tggttgtctc attaataatc tagttgctta 43260
agacaaattt tagtgttcct aaacactaat tgttatctca ttgaactcat ctaaattctt 43320
ccaaatgtta ggtcatctga agaagataaa aagaaacatc tgtgtgtgtg tgcctacagt 43380
catgagttta gaaacagttt agaagggaga agaaatacgt ttagaaggga gaagaaatac 43440
gtttagaagg gagaatgtgc tgtaataatt tgatacaatt ttttacatac tttactcaag 43500
ttctgaaata atagcatgaa aaggacattc tgaaaaagat ggggagggaa ccctacttct 43560
caacagccct ttgcactagt ttatcattcc attaatttga gatagtcact atgtcataat 43620
gaatgttttt aagggcagga aatccaacag ctccccctgg acttgctctc tttcaggctt 43680
tagagatcct ttcttatctt taatgtccat gatgcagcat aaacctactt ccttattggg 43740
tcttcagcag acatggaaaa gaggaacaga cctttccaca gacttgtggt tacagctcag 43800
acttactttt ttctcctaca aagaaagtga ttccctagat tctacactgt ttagttttgt 43860
ttgcttgctt taatattttt aaccttctaa tattttttgt agttattctt tctgcagctt 43920
ttttgtcacc tattaggatg tgagcctccc caaatcaaca tagtaccaaa tgtaagaaat 43980
catttaccca gtcccccatg tattaggtct gtcccccaaa tttaaaatca ctgattcaaa 44040
cacattacct taataagagc agggtatggt gtcgcctctt tttctaacgg taaaatctca 44100
aaattcgtag gaataaattc attctttgta ggaagtttaa atttcccatt caggtcagca 44160
ttttgccatt tctggatgaa taatgaaaca cacaaatgca gtaagagtct gatttaagag 44220
tttaaaatag tatctctgct caatatatgg gcaaatatta agtgcacaaa tgtcacacag 44280
cattgttttc catcatatat aaaagaggta ctaccaaatc tatcgatggg ttttcctgaa 44340
acctgagtta gtctttctag tactagcaaa gattttgcac tcatatatct actcttcctc 44400
tccacatctc caaaatgacc agctgaggaa caattgagac tatggaacac agaaaagctg 44460
actggtccat atgcagatca tgtttattct agctctttaa agaatggaga ataatctgct 44520
caggaatata ttcccacaac tgctagacaa taggtcattt atataagagc catactttca 44580
gctatactaa gttatatcca tgaatggcct gaaagccagg ctaaaggagg tgtctttcac 44640
tttagtactt ctctcttttt tttcttcctg agacagtctt gctctgccgc ccaggctgga 44700
gtgcagtggc atgatctcaa ctcactgcaa cctccacctc ctgcgttcaa gtgattcttg 44760
tgcctcagcc tcccaagtag ctgggactac aggtgtgtgc caccacaccc gactaatttt 44820
cgtattttta gtagagattg ggtttcactg ttgcccaggc tggtcttgaa ctcctggcct 44880
caactgatcc acccacttca gcctcccaaa gtgctgggat tacaggtgtg agccaccatg 44940
cccggcctca cttcagtact tctttttttc tttttcagac agaatctcac tctgttgccc 45000
aggctggaat gcagcagcgt gatctccact cactgcatcc tctacctccc aggttcaaac 45060
gattcttgtg cctcagcctc ctgagtagct gggattacag gcgtgtacca ccacactcag 45120
ctaatttctg tattttatta gagatggggt ttcactgtat tgcccacgct ggtgtcaaac 45180
tcccctcaat ctgcccacct cagcctccta aagtgctagg attacagaca tcagccacca 45240
cgctcggctt tcagtacttc ttaagaactg gcccacacct ctcccagatg atcatgtgaa 45300
cttttaagtg aaccagtagt tcaagcacaa ggttaagaac aaagttgagg ccaggcgcgg 45360
tggctcacgc ctgtaatccc agcactttgg gaggccaaga tgtgcagatc acgaggtcag 45420
gagttcgaga ccagtctggc caacattgtg aaacctcata tctattaaaa atacaaaaaa 45480
ttagccgggt atggtggtgt gcacctgtaa tctcagctac ttgggaggct gaggcaggag 45540
aatcatgtga acctggaagg cagaggttgc agtgagccaa gatggcgcca ttgcactcca 45600
gcccaggcaa cagtacgaga ctctgtctaa aaaaaaaaaa agaaaaaaca aagttgagat 45660
ttaggttggg attcacacaa cctgtagtag actggatctg tttcctgttc tagttccaaa 45720
agctaagtgg gacagatcac aggccactga ttgttgaacc taatttataa ggcttttcat 45780
tatttaaaaa aaaaaaaaaa aaaaaaaagc aactggccag gagtaaaaag caaccagcct 45840
ggacaacaga gcaaggccct gtctatttaa aaaaaaaaac caattgtcaa taattctgga 45900
tttctggatt cagtaatttg atctctagct ggtgtggcac agccttgcag attttacggt 45960
tcttagttcc tgcatttgtg actttcggca ctggggacgt tggccatatt tcttaagtgg 46020
tatcttaggt ttccagaaca gctgaatact agggactagg aaacacttag taagtaattt 46080
cactactgag ttcccagata gatgtgttct taaaaaattc ccagccgggt gtggtggctc 46140
acagctgtaa tcccagcact ttgggaggct gaggcaggcg gatcacaagg tcaagagatc 46200
aagaccatcc tggccaacat ggtgaaaccc tgtctctact aaaaatacaa aaattagctg 46260
ggcgtggtgg catgcgccta tagtcccagc tactcgggag gctgagctag gtggaggttg 46320
cagtgagcca agattatgcc actgtactcc agcatggtga cacagcgaga ttccatctca 46380
aaaaaaaaaa taaaattccc attcagggca ggttggttat ggtcagagtc cagtgaacag 46440
ggggattctt tcttctttta ggactatttc agacccagca ctaattcatg gaagatcagc 46500
tttcctctca gtttagagag atggacattt tcacacactg gagggcttca atgatcttct 46560
gtatcatgaa tatgtcagag atctgtgaag taccagtgat gatcttcttt cttaaactaa 46620
ttcctttttt tgtatgtcat tacacaggta catttttaaa atgtccatta cttgagttta 46680
actagaatct agcctgagta aagttttctg gtcttttccc aggaataagg gacgcagtct 46740
caaaggcaca tagccaatga gaaccaaaag agaaagaaag aaaaagtact agataagtag 46800
gtaacattca caagcaaaat cctgactact gatgaatggg acaaaatatt gttttaacat 46860
accttttctg aaaatcataa ttctcctatg ttgacaaaaa gaacggtgct ataacatgag 46920
gcataaaaca tagcacctcc tgaagtgaag atggtatgaa tcctagagcc tgctctcagc 46980
ctgtgttcac agcaagactg tgagctcttt ggagcccttg cattggtttc tcatgcttcc 47040
tacttccatc gcttcttgcc tttctggtgc ctgtgcccat gtactctgca ctttagttac 47100
attttatggc atgaggcaca accctggtgg tttgatagac acatgagaag gtggctagag 47160
taccatgtct tcatgaaaag gctaaactcc agctccctat ctacttgttg atttctctta 47220
ggatggtaca gataacacta ggtcatcagt cttccacact ctggggcctt agttggtgga 47280
tctagggagc aatgttcttg tgattcctca catgtcaggc ttattcatag atgagttaac 47340
cctaccttga tgacttcatc cggtatagct tcttgtttgt actgggttcc ataccactct 47400
tctgtgcgat cagtttttcc ttcaaaagat ttagaaacta tggcaaccct agagatagaa 47460
aaaacaaaca aaaaaaccct gtaaactcat actgtatata tgaatttaaa gaacctgact 47520
caggacaata taaacaatat ccaagaatgt gctgggcctc ttaagaccca gcaaatcctg 47580
taaggcttag tccaaacaag caatacatga atagaggttt ctgatcatag tccctgttat 47640
cctttagtta cagtccatta tttttagtat gaaaaagtgc tcgcaggaga gattcctcta 47700
ggttgctact gactaaaatt taaaaagaag agtagctagg ggaaaaaaat gtaacaaggt 47760
cagttaaaaa cagtcagtta aaaaacagtc ctaaaaaaag caaagaagtt gcggatgtag 47820
tggcttatgc ctgaaatccc agcactttag gaggctgagg caggatgctc acttgagccc 47880
aggagttcaa gagcaatctg ggcaaaatag tgggacccgt ctctacaaaa aattgtaata 47940
attaaccagg tggggtggca cgcagctata gttccagcta cttggaaaac tgaggtggga 48000
ggattgcttg agcccaggag tttgaggctg cagtaggcta gaatcacacc actgcactct 48060
agcatgggtg acaaatcaaa accttgtctc tttaaaaaaa aaaaaaccaa aaaggcaaaa 48120
aaagtactcc ttcctaggag tatctcaggt gcagaattaa caggaatgac taataaaacc 48180
tgctggctca tccagagatt ttaaaaacgt gacacacata cccaagaaac ttttattatt 48240
gcttgctaca tttgtgctct atgttttttt ccctgccaga gactagcatc aaatatgtct 48300
actacaaata cacaggcctt cctgctacta ggggagaaag ggacaagacc aaaaaatgtc 48360
taccactgta acctctgcct cccgggttca agaactctcc tgcctcagcc tcccaagtag 48420
ctgggattac aggcacccgc caccacgcct ggctaatttt tgtttttgtt tttttttttt 48480
gagttggagt tttgctcttg ccacccaggc tggagtgcaa tggtgcgatc ttggctcact 48540
gcaacctctg cctcctgggt tcaagcaatt ctccggcctc agcctcccaa gtagctggga 48600
ttacaggtgc ctaccacctt gcccagctaa tttttgtatt gttagtagag acagggtttc 48660
accatgttgg ccaggctggt cttgaactcc tgacctcagg tgatccgcct gcctcagcct 48720
cccaaagtgc tgggattaca ggtgtcagcc actgtgccca gcctaaaagc tttttttgat 48780
gcgacagtag aaacagccca gaagagaagt cagatggaaa aaagtgttca aggatgggca 48840
tggtgactca tgcctgtaat cccagcactt tgggaggcca aggcgggaag atcacgaggt 48900
caagagatcg agatcatcct ggctaacatg gtgaaatccc gtctctacta aaaatacaaa 48960
aaattagccg ggtgtggtgg cgggtgccta tagtaccagc tacttgggag gctgaggcag 49020
gagaatggcg tgaacccgga aggcggagca tgcagtgagc cgagactgca ccactacact 49080
ccagcctggg agacagagca agactctgtc tcaaaaaaaa aaagaaaaga aaaaagtgtt 49140
caaggaggga aggcagaagt aaagtgatgt caaaagataa gtgaacaaaa aatgacaaga 49200
ctagaggagc aaaagaagtt ctgaacaagt ggaactctaa gccatccctg tgtagcacta 49260
tcagggacac tccgcctcta ctgcctttta atcattgaag ttggaatcac tacaccctca 49320
ccagtggact caaccacagg ctgggaccaa tcctattttg aatcttgtgg gtcaaggaat 49380
tctagccaat tagcttgggc aacttggtgt aaagtcagtc ctgctattgg ttccacttct 49440
cccttaagtc ccaattccca attcaaataa ttaggtccca attcaaataa tatctgttcc 49500
tgcttgttct cacctacccc cagaggtttg attctagcct ctgaatgttg gcctcacagc 49560
tgaaagccta gtacttgcaa tcaaaactcc cctctgatga tactcattca ttttgagaat 49620
cccaatctct gttccaaata cctgcccctt gttgaacctc cccaacttca acgctagtag 49680
tctgaactac tgtctatttt ttattcattc tagacaaaat actctacctg gctgactgga 49740
cttggtcttg tcatctgctt ttcggtagat atcctaaagc aagatgactc acctgtcagt 49800
atgaccgact aatcttccag ctgtaggtgt tcctgatctg gcctatctgt cactaaccac 49860
taccagttct agaaaacatg ctaaataaga aatacgttcc agagaaagat acagaggaat 49920
cagaggattt gacagataaa agagttctat tattgcgtca gaaggaagtg atgcatgttg 49980
gtagttcaag aatcccgttt gaggtgtatg tgtagagagt tcacagaaat catatagcta 50040
tatgaagggt gtatggtatc tcttaagggc attcaataca tgatagagcc tatgaagact 50100
caggcctagg aactcgggct tccacttttg ttgatttctc agaaaatgaa tggattgatt 50160
ctcaaggaga cttctaaaaa atatactaag tagaagaatc tggcagatgg tcttcccatc 50220
tactcttcca tgaaacgctt gatttctttt tttttttttt tagagacagg gtctcactct 50280
gacacccaag ctggagtgga gtggacttgc cctctgaaac aagaaaacct taaactcagt 50340
tctgagagag aaagtaaaca agctgccaag ccagatgctc tggaagatga ctatctgaca 50400
cagaaagcag acactcaggc tgtgcagtaa tgctcaggtt aaaaaaaaaa aaggagggga 50460
aaaaggcaga ctcatcaagt acagtcctta gggatttgga tagctatata ccaaagtgag 50520
gtgtatgggt aacatttgaa atgttattat aaggaaacag acaagcacag ttcctcagac 50580
taggcacaga tcaaatacca gactaacaaa aagagatacc tttgcagacc aagagttagt 50640
atatcattaa ggaattccag attgaaatat acaatgaaag ggtatgtatg aggtaataaa 50700
taaagacagg gaagaaagta acggtacaga cagagtatac acacatcaaa cccatataga 50760
tacctatgaa ccagagtgtg aaagtacagg gaagagcact tgaattagga gaaaaggtca 50820
caataattga agtacagaca cctatagtat acacaaccgc agacagttga gaaaaactag 50880
tacaaatatt gtagcatttt agaccatctg ccgaaaatct aattatacca aaaaataagg 50940
tttccttttt tcgagacagg gtcttgtaca gtggtgtgat cataccttac tgcagccttg 51000
aactcctgag ctcaagtgat cctcctgcct cagtctctca agtagctggg actacaggca 51060
tataccacca tacccagcca attttttttt tttttttttt ggtagaaatg aggtctcacc 51120
acgttgccca ggctggtctc aaactcctga gctcaaacaa tcctcccacc ttggcctccc 51180
agagtgctag gattacaggc atgagctacc atgcccagcc cagttttttt ttttttttta 51240
atgtggctag agtgtggggg aagaaaaggc tgccaatatg gtaatgatgt gatccttaga 51300
aatgagagaa caggccaggc acagtggctc aagcctgtaa tcccaacact ttgggaggcc 51360
aaggcgagtg ggtcacttaa gcccttgagt tcaaggcaag cctgggcaat acagtgagat 51420
cctgtctcta tttaaagaaa aagaaagaaa ttaaaaaatg aaacatgaga aaacacaacc 51480
ttttcatact aaaacttaca acagcaagaa aaggaaacag cgggctagaa tgaatgctgt 51540
tgacttcaag tacatttaaa aatgtttgga gcgaatgcag ctgtcgaagc tggatggaac 51600
tcatctggaa acaaaaggtc tcctgactac agacatgaaa aggcaatatg gttcccaaag 51660
aataaactct cctgagattt ttacaaatga tgcctaacac agaataggga aaagatgtcc 51720
aaagaatgag aagaggcttc ataggtaatt gtgattaatt caaacaatta gtggaaaggg 51780
cacagacctg gcaataagaa actgagattc tggcagatac tctgaatcac tttctttgaa 51840
atcttgccaa aatcacagat ctaagttagg cacaagccat ttagcctctc taaacttcag 51900
tttcattaat ttttaatttt tctttttttt ttttgagacg gagtcttgct ctgtcgccca 51960
ggctggagtg cagtggtatg atctcggctc cctggaacct ccacctcctg agttcaagcg 52020
attctcctgc ctcggcttcc atagtagcta ggattacatc acactcggct tttttttttt 52080
tttttttttt taagatggcg tctcactctg ttgcccaggc tggagtgcag tggcgcgatc 52140
ttggctcacc acaacctccg cctcctggat tcaagcgatt ctcctgcctc agcctcctga 52200
gtagctggga ctacaggcac acgccaccat gcctggctaa tttttgtatt tttagtagag 52260
acagggtttc actatgttgg ccaggctggt ctcaaactcc cgaccttgtg atccgcccac 52320
ctcggcctcc caaagtgctg ggattagagg catgagccac tgcacccagt cttttgtatt 52380
tttaatagag acggggtttc gccatgttgg ccaggctggt ctccaactcc tgacctcagg 52440
tgatccaccc accttggcct cctaaagtgc tgggactaca ggcgtgagcc accacacccg 52500
gcctcaataa tttttaaata gggattcaaa ttagatgaac cctctccttc cactagtaaa 52560
attctacact atcatgatat caaattagag ttttaaagga tacacacaaa agacagaaag 52620
ttgaaaaaaa aatctattga tgagattcaa aaatcatact taaatagtga taggaaagct 52680
aaagtgctaa aggaattgag gctcatgaaa aatgctaaaa accatttttt aagtgttttt 52740
tttttttgag acagggttgc tctatccccc aggctggagt gcagtggcat gatcttggct 52800
cactgcaacc tccgccccct gggttcaagc gatttccatg cctcagcctt ctgagtagct 52860
gggattacag gtgcctgcca ccatgcccgg ctaatttttg tatttttagt agagatgggg 52920
tttcaccatg ttggtcaggc tggtcttgaa cacccaacct caggtgatcc acctgcctca 52980
gcctctcaaa gtgctgggat tacaggcgtg agccaccacg cctggcctaa agtgtttctt 53040
aaagtaagaa aaaagtcaag aaagattagg cttaaagttt agaaaattca gtcaactttt 53100
actgagttct taccaggtca aattctggga aagacaaaat atagttccta tttggaaaaa 53160
ctcataattt aataggacac atgacctata gcaaaatgta atcagttaat ctcatgttaa 53220
cagatggcag gactactcct ctcttattta cctggtcttt gaaataaaca tggctcttta 53280
aactgtaaag gtggaccaaa cacagaaaaa aggagttgaa gataggtgaa tgagccaagt 53340
atgaaaccac ctctctagcc aggtgtggtg gctcatgcct gtaatctcaa caatttggga 53400
ggctgaggcc ggtggatcac ttgaggtcag gagttcaaga ccagcctggc caatatggtg 53460
aaaccctatc tctactaaaa atacaaaaat tagccaggtg tggtagtggg cacctgtaat 53520
gccagccact cgggaggctg aggcaggaga atcctttgaa cccaggaggc cgaggctgca 53580
gtgagccgag attgtgccat tgcactccag cctgggcaac agagcaagac tctgtctcaa 53640
aaaatataaa aataaaaaag gagaaatcac atctccaaac acagataagt cacaatttct 53700
aatgcttagg caactaccaa atcctaacag aatcattgtc catcatcttt tagaaatgct 53760
ggagaacacg agatgaggca gaaaactgga gatggagatg ggcaaatgct gtaactttca 53820
aaaagaaaaa aggagtgaag cctgaacacc ataagccagt aaattaggaa ttgatccctg 53880
acaaaattct acagttaact gtcagactgt cagacattta gacaagaaaa ccgtcgttat 53940
cactaggact ttcatggact aattaagaat aacttctccc aaataaatgg aattatttat 54000
gactaaagga aggcacatga caaagactct atagctgtct ttgtagattt aatatggtaa 54060
aacaggcacc aaatgatagg atcaggggag gtgggtgaca atgatcattg agtaataata 54120
ggtaaacacc tttgaacagg taatcaagtc tcaaaattca aaataattgt ctaacaatta 54180
ggactggcaa aaatgaaact ggctaccttg gtatttaatg agttccccat ccttggaaga 54240
agttaaactg aagctggtaa ctacttagct gggatactgc agaacagata cacagtacca 54300
tactacacca tttaatataa gggacttgag caccttggat tttgatatcc atgggggtct 54360
tggaaccaat cccctgcgga tatggagggc caagtgtata cctaacagga gttggattaa 54420
atggcctcta aggttccttt cattcttaag agtttcctgt ttatttccct acctggttgc 54480
aatgttaaac ttgtctcagc gggtattaaa gaaggtaatc ttacccttga gccaccctag 54540
tctggtctgg cagctggcaa tattatccca ggctgttttg caccatgtat cactgaccta 54600
ctacagttac aactgttaac agtcacagag accaatcctg tagaggaaaa gtgctttctc 54660
tttaaatgtg agagtaaaaa gaggtattta attgcataag atatcaaatt tagaaatatg 54720
caatttcaaa agtaaaacat atgctccagc aaatgcactc ctacgaagtg ttgctgggtc 54780
aattactggc tctagagcag caagaattat aatttaaatc atggtttttt cttacaaaaa 54840
tgaaaaagca catccttatt atatggtata actttaatat agcattacct tgtgtttgta 54900
caacactttt tgactttgct aaatcatttt acatcataca gataaagcac tatccccact 54960
ttgcagatga gcagggttca aggaaattaa atgacagcca gtattcatga cattatataa 55020
agttaacttt tcacaagtcc ccctttcagg actgtcaact ctgaggtttc acgtaagtga 55080
ttcaataaac attgaaagaa aaaaatgatg tatccaagat ctcatgacta gtagtaggtg 55140
acaaaaccac acaaaattag taactggaag gaatcttaaa gattgtctag tgaggccggg 55200
tatgggggct cacacctata atcccagcac tttgagagga cggggtgggt ggatcacttg 55260
aggtcaggag tttgagacca gcctggccaa catggtgaaa tcttgtctct actaaaaaaa 55320
aatacaggcc gggcatggtg gctcacacct gtaatcccag cactttggga ggccgaggca 55380
ggtggatcac ctgaggtcag gagttcgaga ccagcttggc caacatagca aaaccccatc 55440
tctactaaaa atacaaaaag ttagctgggc gtggtggcag gcacctgtaa tcccagctac 55500
tcgggaggct gaggcaggag aattgtctga acccgggagg cggaggtgca gtgagccaag 55560
atcgtgccat tgcactccag cctaggcaac aagagcgaaa ctccatctca aagaacaaaa 55620
aacaaaaatt agtcaggcat ggtggcgggt gcatgttatc ccagctactg ggaggctgag 55680
gcaagaaaat tgcttgaacc tggaaaacag attgtctaac aagttctatt agtaaatcta 55740
tagctctttc tcctcatgtt ggtaagaggt tttatttgta gaaggtatat gataaaattc 55800
aaccggattt aggctgattt ttcccccctg gaaatatagg tacactgagg aaaatacaaa 55860
tatttaaact gttttacttg cctaaatgag gcaagtaaat taaaaccagt gtggcaagta 55920
aagtaaaaat tagtgtgtgt tctgggtatt tttcttcaat gtattttaat gatgtaagta 55980
aatgcatgta tcacttaaga gcggttttta atattgatga agtccatttt attggtggtt 56040
tttctttttt ttttgagatg gagttttgct cttttgccca gggtggagtc aagtggcatg 56100
atcttggctc actgcaacct ccggccccca ggttcaagcg attctcctgc ctcagcctcc 56160
caagtagctg gaattaccgt agagatgagg ttttgccatg ttggccaggc tggtcttgaa 56220
ctcctgacct caggtgatcc acccactttg gtctcccaaa gtgctaggat tataggcatg 56280
agccaccgca cccagccttc tttaagacag ggtctcaccc tgttgcccag gctggagtgc 56340
agtggtatga tcatggctca ctgcaacctc cacctcctgg gctcaagtga tccttctgcc 56400
tcagcctcct gagtagctgg gactacaggc acgtaccacc acaccctgct aatttgcata 56460
cttttttaga gatggggttt catcatgttg cccaggctgg tctcgaactc ctgagctcaa 56520
gcaatcctcc ctctatgcct cccaaagtgc tggcattatg ggtgtgaacc actgtgcacg 56580
gtcaatgtgt tttcgttagt ggttactgct tttcatgttc tatctgagaa atctttgcct 56640
atcgcaaggt tttgaagata tttttctatt ttttctggaa gttttataga tttagtgttt 56700
atatttaggt ctatgatcta tctcaaattt tataaatagt atgaggcaaa ggtcaagttt 56760
catcattttt catatagata cgcagctagt agaacagcac catttgttga aaagattttc 56820
cattctcaat tatgttgatg ctcttctcaa aaataaaatc aactgactgg ccgggtgcag 56880
tggctcatgc ctgtaatccc agcactttgg gaggccgaga acggtggatc acctgaggtc 56940
atgagttcga gaccagcctg accaacatgg agaaaccccg tctctattaa aaatacaaaa 57000
ttagccagac atggtggtgc gtgcctgtaa tcccagctac tcgggaggct gaggcaagag 57060
aatcgcttaa actcgggaga tggaggtttc agtgagctga gatcgcacca tggcattcca 57120
gcctgggcaa caagagtgaa actccatctc aaaaaaaaaa aaaccccaac tgactgatag 57180
atgaatgcat aaagaaaata tggtatatac atataatgga acactattta gccttaaaaa 57240
gaaagggaat cctgtcatat gctacaacat ggatgaacct tgaagacatt atagtaagtg 57300
aaatgaacca ggcacagaaa ggtatgttgt atgattctac ttacattagg tatcgaaagt 57360
agttaaaccc atagaaacag aatgtagaat agtgattatc agaaggcaga gagaaaaggg 57420
aagttgtttt ttaatcacaa caaaaaaatc aactgaccat gtatgtgtgg aactatatct 57480
ggactctgtt ctcttccaat gatttctttg tctatccata tgatgtcaat actaccgtgt 57540
cttaattact gaagctttat actaatgtcc tcaaatctag tggtttaatt cctcccacat 57600
ttggtgattt tttttttttt ccggagtgtt ctgacttatc taggtccttt gtatgtccat 57660
ataaattttg gaaccaattt ctgaaaaaag gaaaaacagc ctggccaaca tggtgaaacc 57720
ccatctctac tgaaaacaca aaaattagct gggcgtggtg gcatacacct gtaatcccag 57780
ctacttggga ggctgaggca agagaaccgc ttgaacctgg gaggcagagg ttgcagtgag 57840
ctgagatcgc accactgcac tccagcctgg gcgacagagt gagatcctgt ctcagaaaaa 57900
aaaaaaagaa aaagaaaaaa agaaaaaaaa ggccaggtgc agtggcacat gcctgtaatc 57960
ccagcacttt gggaggctga ggtgggagga tcatttgagc tcaggagttc gagactaatc 58020
tgggcaacat agtgagactc tctctctaca aaaaaattaa aaaattagct gggcattggt 58080
ggcacatgcc tgtaatccca cctacttggg aggctgaggc atgagaattg cttaaaccca 58140
ggaggaggag gttgcagtga gccaagatga cactagtgca ctccagcccg ggcaacagag 58200
caagactctg tcattaaaaa aaaaaaaaaa aaaaaaaaaa aaaagccggt gcattggctc 58260
acgcctgtaa tcccagcact ttgggaagcc aaggtaggca gatcacaaag tcaagagatc 58320
gagaccatcc tggccaacat ggtgaaaccc tgtctctact aaaaatacaa aaattagctg 58380
ggcatggtgg cgcgtgcctg tggttacagc tactggggag gctgaggcag gagaatagct 58440
tgatcctggg atgtggaggt tgcagtgagc cgagattgcg ccactgcact ccagcctggt 58500
gacacagcga gactccgtct aaaaaaaaaa agaaaaaaga aaaaaggccg ggaacggtga 58560
ctcacgcctg taatcccagc actttgggag gctgaggtgg gcggatcacc tgaggtcagg 58620
agttcgagac cagcctggtc aacatggtga aaccccatct ctactaaaaa taaaaaaatt 58680
agccaggtgt ggtggcgggt gcctgtaatc ccagctactc aggaggctga ggtaagagaa 58740
ttgcttgaac ctgggaggcg gaggttgcag taagccgaga ttgcaccatt gcacttccag 58800
cctgggtgac aagaatgaaa ctccatctaa aaaaaaataa taataaacaa acaggtatag 58860
tggcttgcac ccataatccc agctactcag gaagctgacg caggagatca cttaagccca 58920
agaggtcaag gctgcagtga gctatgatcc cgccactaca gtccagcctg ggtgacaatg 58980
caagaccctg tctctaaata aatgaataaa aagaaaaaga aaaacagcct accaggattt 59040
tggttatgat tacattgagt ctatgaatca gattggagag aactaacata ttaataatat 59100
caagtctcct aatgtatgac tatagtatgc ttctccattt atttaggtct tctttcattt 59160
ttctcggcta atatttgctc tcttctactg tttttagttt tttatgtaga agtcttgctt 59220
atctttggtt aaactcattc ctagtaatat tatgcttttg ttgctattat aaatacttct 59280
tttaattttc tatttttttg ttgctatata tagatatgca gttgattttt gtatgttgac 59340
cttgcaacct gtgactgtgc tgaattcagt tattggttcc agatttttga aggggaaagt 59400
agattcctta ggattttcta catatgcaat tatgtgtatc agtgcatata tcactttaaa 59460
ccatttaaat tatgtgaact atatctcgcc atcccccact aaactacgtc aggcatgaat 59520
aacttaacat attacgttat acaaatattt aatctacaac tttctctgtt gttcactgaa 59580
tggaaaagga aagatttctt cgtttttcaa ttcctcagta aattatctag agttgaaaaa 59640
gagcatttgc tgttaaaggc agaatgatca actcagcaat ctctaatgaa ttcacagctg 59700
tcagtaactt tgtctagttt ttcagtaaga ttttctgtat cataatcaat tcttgagtaa 59760
ttaaggatta ggcaataaat ggggacttaa aatattggca agaaactgcg taggaaacat 59820
tgtaacctac tgggtacctc aaaccagaaa tttaatttct aaataatttc acatgttatt 59880
ttcattgaaa aagacttcta aattttgacc cttaatatat tttttaaacc tcaaaaccat 59940
ggtatcttat tccaatttaa aaattctagg ctcagtaaga aaaaagaaaa gaaaattcta 60000
gactgggcac agtggctcac gacggctgta atcccagcac tttgtggggc taagccggac 60060
tgcttgaggc caggagtttg agatccacct gggcaacaca gtgagaccct atctctataa 60120
aaaattgttc ttgaagctag ataattatct gtactcatcc aaacactgta tcataatata 60180
aaaaaatcat taaaactttt taaatacaga tagggtgtca ctatgctgct caggctggtc 60240
ttaaactcct agtctcaagt gatcctcctc tgccttggct tcccaaaaaa tagaatattt 60300
agctgaatcc tagcatgagg tcaggtaaaa tctggctaat agtatttcct agaagaaagt 60360
cacccgagtc caccaaccac ctaggagtga caatagatag gccaagccca cctgaacatt 60420
agaagacatt aattattgtt ctcaacctaa acagtatagt gcaagtcata tatatgcaat 60480
tatcgctaat gaagctttga taatatgtct ggaaaagttc aaacaatggt aactaggaaa 60540
agattctgat aggatagttc tgtcaaaaag actttttttt ttttttttga gacggagttt 60600
cactcttgtt gcctaggctg gagtgcaatg ccatgatctc ggctcacagc aacctccacc 60660
tcctgggttc aagcgattct cctgttgcag cctcccgagt agctgggatt acagatgcat 60720
gccaccatgc ctggctaatt ttgtattttt attagagacg gggtttctcc atgttggtca 60780
ggctggtctc gaactcccga cctcagctga tccgcccgcc ctggcctccc aaagtgctgg 60840
gattacaggc aggagccacc gcgcccagcc caaaaagatt tttttttttt tacaaaaagt 60900
tctatcacat tgttgatctt attcataaaa caactgttcc aaaaaactta ctcaataacc 60960
aaattgccag tagaatttca aaaatacttc cctaacagag gagaaaaatt aaaaatgtaa 61020
aacatgtcat tcaattatat tccctctatc atttgacttt agaaaacata gatgaaattc 61080
agttttatgg gaggaatatc atgtggtggc tcacaagcac tttaaggact actttcctct 61140
agcaagaata cctctgctac tataagagtt tggacttttg atttccaggt taaccactgg 61200
tcaatcagga ggattctgag attgtaatct ttactgtggc ttaagaaaaa gagggtgcct 61260
tcatttgctt atatgagtca gataggtggg ctgatacttt gggcgtttga gttagaataa 61320
agccaggtta agacaggtta ttattctatt ttatttatga taataaaatt atatttgttt 61380
ttaaaaatgt cctcatcttt tagagatatt gcctcaaaat aatttagggt ggaaggggga 61440
tgactgaaga gggatgggta tagattaaaa aagactggcc atgtatcaat ggttgaagct 61500
tggtgttgct tcattatact attctctact tttgtaatgc ttgataaaat gtttaaaagc 61560
attttattca caatttcaaa ttttttcaga ttctaatcat tattgaaaat ttagtgtata 61620
atagtaaaat ctgtatggtg actcaccgga cattttctgg tctgagttta tcgagaacca 61680
tctctattaa gtcaggtcta aattcttcca gtaaatattc cgctgtgagc acctcttcta 61740
ggggataata cttttaaaaa ggaaaatata aataaataaa atatacaaag ctactacata 61800
gtttattaaa gcagatctgt aacacattaa attcattact gtacaccctt tctctcttca 61860
gttaaatttt actccattgg aaaaattttc tgtagcccta aatcgttgtc tctttcctac 61920
cacattgtta agcagtttca tattgtttga tgattctgcc aaatatactg ttaatcccca 61980
aacatacttc cattgtagag agcaaaatca catttctttc ggggaaaacc acgaatgttt 62040
ctggttaaaa actgagtact tgttaatgaa gaaaattcga tattaaaatg ctgacaccta 62100
caggattttt aaaatataaa gtacacacat ttttatgaac agaatttcct cctaaatttc 62160
tatgaaacaa attagacaaa catttaggaa gcggctgtgg aaaaaaaaat ggcttatatt 62220
tttggccagt gtagaaaaac aatgcagaag atgctttttg gtaagagcaa cttccaatat 62280
actgagacac actgactgat tcattcatct aataaatatt taatgaacat ctactatgtg 62340
ccagacactg ttccacacac tatggataca aaagtaggta agacacatga aatcctttgt 62400
cctccaagaa gcacacagac attgtaattg ggagggaggc agacaacaaa caaatattgt 62460
aatgttagtg agagatgcta tgaaaacaat aaaaaaaggg gcaggggcat tattttagat 62520
tgggaggtca gagaaggtct gaggagaaga actctgagtg gagactgaaa tgataagaac 62580
ctagccaggc aaagatcaga tagaacagtg tttcatgtag agaaaatagc aatgggggag 62640
gaataaactt aatgcttgaa aattagagag ccagtacagt tggagtggaa tgcaaatggg 62700
agaggtgggc tggacagtgg tatagaagat ggccagatag gctggggcca catcaccttg 62760
taggccaagg aaaattttgc attttatgcc aactctgttg ggaaaccact gaaggaattt 62820
aaacagagga gtgaaacatt cagattttag ttttcaaaag atcacgagcc tggaaaaata 62880
aactacaatg gggaccaaga ggaaaatagg gatcgcaggt aaaagactat catggtagcc 62940
caagcaagaa atgatgatag ctcagactag gatggtagca atggaaattt taaaaaagta 63000
aatgatcaat agctttcata taagtaacac ctgatatgct gtagaactga aatatattta 63060
aagccaataa gcaaaatgca tatttaataa aaatattaca taaaataaat ttcacgatga 63120
tgaaaattaa gattttaaac atctcctgac atcaacattt gccctgttgc tacaaagttg 63180
aaagctttat acctcagctt caattgtaat cattcactta ttactgaaaa tatatgcaag 63240
attgctgaaa tactccacac agcaatgtat acagtaaaat aacaacaaac ttacatgcaa 63300
tattcctgca atcttagatg tatagccccg tggcctctct ttgtctttaa acctaaaagc 63360
aacagcattc aagtcctaaa atagaaagtt aatccaatta gatacggcca cccttattta 63420
gaaacctttt tttttttttt taagtgtttg gcttgcacta taagctttct actccatatt 63480
taactttatc acactttctg aaaaaatggc tatgcttgat ttagaaatgt atagaatatt 63540
ttaaaaattt cttaatgtgt ataaaattta atccatcaat ctaggcttaa aaccaaaagc 63600
aaaataggta aacaactttc aatgaaaata acaaatacag gatatggctc gatagcctaa 63660
gttgttgaaa ctaatagcca aagaatttag aaaaactgtg tgaaagaacc ttgtgagatc 63720
caacaattcc accatagatg attttggtga ctcattcaga gaacagcacc cagattagga 63780
gttattgtca taattttcag aagcagagat tataccttta aacttttatc tagttaaaaa 63840
ggatgtttct catactaaaa tacatttgag aatctcgata ggctttaaag cacgtatttt 63900
taaaaaccct tttttaaaag gcttttaaaa atttgaaaga caaacaccat ttgttttacg 63960
caaaaaaaaa agccagcctc ttcccataca tacaataata aaatcacttt taataacaca 64020
atcatacata aagcaattca ttacttcttt gagagtttgc tacagattta aagatctgaa 64080
aacgacagag ctcatgcagc ttcaggcagg gagtaacata atccctgaca tattccagct 64140
caagaaactg ctgattttct tctgagtgat ggaaaagcat ttgatttctt cccttttcca 64200
tcctaaaaag ggctacacga tgcctttccc cttagttgtg ccccaagtag caaatcagac 64260
tagtacaact aagccttaac taatactatt atacattgca aaattttaga cttcagttag 64320
gaaaaaaact ttaaaagttt aactgggcct taaatttaaa ttaattcaca tgtatttgct 64380
cttgaaaaac agattcctta agaaaaattt tggtgaaaca aaagtacctt gcactcttgg 64440
aaaacccatt cttgaggtcc ttctgcacgt aacttctgaa tgtattgaaa catgtgcaaa 64500
attatatctt caacatgtac tggaaaaaag gggcacactt aaaaaccatt cagtccttga 64560
atccttcaaa gcagtgagct cactcctaag gtaaagtcag ataagtggaa gatggtccct 64620
cttagaaaat gcagtcctca cctccctgag actggcttta cgtgtcccac atacatggcc 64680
ctctgggctc agagatatgt gacagaaagg gtgttggggc agtaaatgag caaataaaaa 64740
gccaagtcca catttaacaa gtgcaacata atgatcagtt gttaaggggg gaagaatcat 64800
ccatgaaaca aaggccaagt tacatatgaa aaatacaatg gataagttgt atgaattaaa 64860
gtgttgtagc tttcaacata aaacagatcc acagcatgaa gaagttaaaa aacatcatag 64920
aaagtaaatt ttcattcaaa agtaaaaagg tgaatcaata cttacataat ccttcctcgg 64980
tcaagtccac attaatgata aaaaacataa aacctcgggc tccttccttc tgcccaccaa 65040
caagagtatt aacccagcct gcaacattca aaggaaatca atacgattga ttgcatatgt 65100
ggcttccttg aagataagaa aaattttaaa aattcatact gtatgttcct aagatatcag 65160
aatgaacctc ttgcgaaaag attgggaaaa aaaaatcagt tcagaaaagc agtttaagat 65220
ggtgcagaag tgggggcttt cctgaagatt cctcaaatca attcccagtc aagagtagac 65280
tactggctgg gcgcggtggc tcatgcctct aatcccagca ctttaggagg ccaagatggg 65340
cggatcgctt gagaccagga gtttgagact agcctaggca aaatggcgaa accccatctc 65400
tatctaaaaa agaaaaaaaa aaaaaaaagg caacagcgtg ccaacaacca ccatctcact 65460
cactaaccca aaatggtcac atctaacaat gttccttcat aggacactat tcaggagaaa 65520
atgagagcca gagtgaaaag atgtggtgga cactcagaag atgctgccca ggagacttac 65580
cctttgactt aagttctgat aacagacttc caggaccttc atgcccaatg agatgaccaa 65640
gataatgacc aggatttgat ttgtagtatt tctgaaggtc aggtatggga aatgtcacat 65700
agagattcct aatatcttta atgggtacta ttttgtaaag ttgctggaga aaacaaatca 65760
cagagattag ctatatacga ctcctactga agaaaatatt tctaaactat gaagaatgat 65820
tttcatggga gaatcccaaa tttaagagca aatcaattgc cacagaaaat gatacactga 65880
ccacaaagaa ataaatgact cagcactctc ctagtggaat ttcattacag tcggtatata 65940
gtccatgttt gaccccctga accatgcagc taaatattaa tggttttgaa tgcttatctt 66000
caggtacagg ttaacagctt caacagtcta ttaagactac agctaccaaa actactttta 66060
gcatgtaaag accagaacat attgatatgt ttttggtgat tcatgatcct aataaatgtg 66120
agattgattt tattatcccg acaaatattt aaaaccacat gggggccggg tgcggtggct 66180
cacacctata atcctcgcac tttgggaggc tgagggagac agatcacctg aggtcaggag 66240
ttcgacctca gcctggccaa catggtgaaa ccccatttct attaaaaata caaaaattag 66300
ccaggcacag tggcatgcac ctatagttcc agctactcag gaggctgagg cagaagaatt 66360
gcttgaacct gggaagcaga ggttgcaggg agccacgatc gcgccgctgc actccagcct 66420
gggcaacaga gtgagactcc atgtcaaaaa aaccaaaaac agaaacaaac aaacaaaaaa 66480
cactacatag gggctgggtg cagtggctca cgcctgtaat cccagcattt tgggaggctg 66540
agacaggtgg gccacttgag atcaggagtt cgagatcagt caaggcaaca tggtgaaacc 66600
ctgtgtctac taaaaataca aaaattagcc aggtgtggta gtgtgtactt gtagtcccaa 66660
ctactcagaa ggctgaggtg ggaggatcaa ttcagccagg gaagttgagg cttcagtgag 66720
ccatgatcgc accactgcat tccagcctgg gtgacaatgc aagactctgt ttccaaaaaa 66780
aaaaaaaaaa aacacaacat aaataatgaa acctccaaaa attttcacct gttctatggt 66840
aaactaggta tatattatgt ccataaatta agaagacaaa ataccaaaat ttatgcactt 66900
actttaagat gttcttcttg gaaagggtgt tcaggaaatt ctggcaatgg aacatttttg 66960
ttctctactt cagaaaataa ctttaccacc agattagtca agtcatctaa agattctaca 67020
agaaaacaga caaggaaaac agaacttaag cgaatcataa catccaacag ggagtgctta 67080
aaatcttagg atctcctgaa aagtctcagt actactgaaa ggaaaatgtg aaaatgatta 67140
cacctcacac ctatctgttt ctactttaaa aaattattca caaatattat tcaaaatatt 67200
atttcacaag tattataata aaattaaaac aaatattaga aacaataata attgcatttg 67260
gaaaaatgac tatgattccc aacaggcata tatactgaac tactagcaga tagagtataa 67320
tgttcacctt atacatagac agagaattgc cttctatggt ataaaattat cttttgtgcc 67380
tttaaatgta tttgcatttt atttataaaa gacgcctatt tttactttag ttttttgaca 67440
cgtatgaagg actccaaaat gcacatttta tatgtgtgtg tgtatatata tatcacatat 67500
atgatatata tcacatacat ctcacatata tgatatatat cacatacatc tcacatatat 67560
gatatatatc acatacatct cacatgtatg aaatatagca catacatctc acatatatga 67620
tatatagcac atacatctca catgatatat agcacataca tatcacatat atgatatata 67680
gcacatacat atcacacata tgatatatat cacatatata tcacatacat gatatatcac 67740
atatatcata tatatgatat tagcaaaatt ctacttttgg aagtataaca agaaatgagt 67800
tagatctttg agaagagggc acagcagtca taggatggaa taaagaacag cttgcttttt 67860
attttatacc tttctgtgtg gaattaaatt ttttccattt gtacaaatta ctattatcct 67920
aaaaagaaag cagtgttaaa aaaaaaaaaa aaaacttttt tttttttttt ttgaagcaag 67980
gtattgctct gttgcacagg ctggagtgca gtggcacaat cccagctaac tgcaacctct 68040
gcctcccagg ttcaagtgat tctcatgtct cagcctcccc agtagctggg attacaggcg 68100
tgtgccacca caccctgtta gttttttgta tttttaatag agacagggtt tcaccatgtt 68160
gcccaggctg gtctcaaact cctgagctca agtgatctgc ctacctcagc ctcccaaagt 68220
gctggaatta cagatgtgag ccaccatgcc tggccaaaac ctgtcttttt ataaacaaga 68280
taacctctca tcttgattct gtctcctcaa aaataggtta tttataaatg gtataaacat 68340
tcataacctg ttactgtcag taagcatttg aatcaaataa gattcttgcc ttgatattct 68400
atgacccatt tggaactttt ccatgaattc tcgagagtac taaatgacta tttgagagct 68460
gcctttatat ggtgtaaaat tctcttttgt gcctttaagt gtaggcattt gcattttatt 68520
tataaaatat acctactttt gcctcaattt ttttgacatg tatgaaggac tccaaaatgt 68580
ccagagccaa ataaaaggca gatcagtggc tgcctattat atgacaagtg aatttaggaa 68640
tggagaagga atattgtagc aagagatgtc agagacatct ttttgcagat gggacatatg 68700
acttgaaaca aggtgagcag tcaataaagg tttgtgggcc tgcagtactg gtgacagcaa 68760
caccctgagc aaggttctac caggtgtatc tttcctcttg cgtatttcta ccctaaaagc 68820
ataaattcac agtgtcactt tttaaaggta agggagctaa acactattgc acagtctgtc 68880
taccccacta aactgtgagc tccccagaca tcagggaact gagtcccact caccttcata 68940
tcatcaaggt ttggcaacct cagtgtctgt acttgatgat tacctatgtg gcaagtgttc 69000
agataattag tgcttccaca actagggaga cagctccagg caagaacaga aaaacttttt 69060
gttcccttgt gacagagatc cttccacatg gttactcttc ctctctcctc ccgtcaaaag 69120
aaggagtgga ttatctttta atcctccaag agatccagtt tcctcctcat acctctatac 69180
tgtaaagaca tctctctctg gttcagcaca ggaagtcagt cacaatggga gagtgatgtg 69240
tatggatcag agaattgaga cactttattt tttagattct tattctgtca tttccactaa 69300
accaagaaca ttctgaggac agggatcagg tttgattcat ctattgccat tgttacttgc 69360
ctattaaatg tttactgaaa tcatgtgttt aatctcaaat acaacataga ttaaaaaaaa 69420
aaaaaaacac ccagctttgg cacctaatcc caggactggg ttctaaacag caagtgatat 69480
tttaacattc taaacccacc tacctgaagc aagcacatag cattacagag tatgcttttt 69540
ttttactgtc ctcactccct gaatccattt tacaccctgg ttcttgctac caacttaaaa 69600
aatagtttat tttctaacta taaaagtaac atttgccaat agcagaaaac ttagaaacaa 69660
aagctaaatt atggtaacaa aagttaccat aatttcatga ttataaaggt gctagagtgg 69720
ctactactgt ttatggagca tttgctatgt gccatgcacc ttgctaaaca ctttgtatta 69780
tctcaattaa acctcacaac aggtacaatg taccacccac cactttacaa atgtgaaaac 69840
tgaagtttga aaaacctaag taagtttgtt agctcatagc cacacagcta agtaagtggt 69900
atatttggaa tgaaaagcca ggttgttgga ctgtagaatg tgagttctta attactatac 69960
aacattatta aatttctagt acatgctttt ccattttgtc ttttacctac accatatatg 70020
gtatattaga gtttttggtg ttttttgttg tcgtttgttt tttgagatgg agtctcactc 70080
tgttgcccag gctggagtgc agtggcacaa tcttggctca ctgcagcctc caccccccag 70140
gttcaagtga ttctcctgcc tcagcctccc aactagctgg gattacaggt gcccgccacc 70200
atgcccggtt aatttttata tttttagtag agatggggtt tcgccatgtt ggctgggctg 70260
gtctcaaact cctgaccaca agtgatccac ctgcctcggc cttccgaagt gctggaatta 70320
caggtgtgag ccactgtgcc tggaccctat tagagttttt aaaaattttg tgggggggat 70380
atgtcttgga atttttaata aatcccaaaa tgggaccata tagcacatgt ttttgtaaac 70440
agcttttgtt ttctctttaa tatattaaga acattttaaa aattatattc ttatatataa 70500
tatacatata ttaattaaaa atgtttagtt atatatgcac atataagaac atatattcat 70560
atatatgaga atatattaag aatatcttcc catggtatta aaatgctgtc ataaaactgt 70620
ttattcattc aatatatatt tacctatgat gtgccaagca cgagcccagg tactgaggat 70680
aaagcagtga acaaaacaaa gtttctattc tcaagtaatt tacactttgg tagggtggac 70740
aacaaaaaca cacaaatatt taatacctga ggtattaata ctataaagaa aaagcaggta 70800
agatggaaag tagaatagga aatgttattt ttctataaga tattctattt gcaattataa 70860
aatcagtatt tagccaatct cctattaaca tttagatttc taaattttcg ctaatatgta 70920
ctatactgac agaaacatcc ttttatatct cctatttgaa ctctgattat ttcctgagat 70980
tgcattttta aaagtaaaaa tgcatcttta cttttaagac tgatactgtt aaactgccct 71040
ccagaaagac tagagcaatt gataccatca ttaccaatat aaaaaagcaa caccatcggc 71100
agtgggtatt acttcaaaac aaaactgtga taatttaaca gcaggaaaca atgttagtag 71160
ttaatctgca ttttaaaaat caaaccattg tttacattcc aaatggtttc ccaagtttat 71220
catcatctgc tttttttttt tccagttttt tgaattgtta taaggtcaaa tctgccaatc 71280
tttgtccttt ttgtgtgttt ttttttaata tgagatctct ctctgccacc caggctggag 71340
tgcagtggtg tggtgatcac agcctttaac tgcagccttt aactcctggg ctcaagcgat 71400
cttcagcctc cctagcagct aggactacag gtgcagacta ccacatccca ctaatttaaa 71460
aaaatttttt ttggtagaga caggatctta ctatgttgcc caggctgggc ttgccttgct 71520
tttatatgct tagagcacct attactgtag taatttgtat tttttttcag atctccactt 71580
aaacttcttt tgggaacaca atgatttaaa cataaaaatc cccacctatg tattctctaa 71640
caagtagatc attttcaaaa gatgctgagc aaataagcag ctctatctta tatccatctt 71700
ctaacactgt gaaaaagcca acattaaaaa cttatattat ccaattctat aaaaaattgt 71760
aagggttctt acctcgacct aaaacacaaa cagccattaa gttggatgaa tagtaagcag 71820
aatggaattt cagtagctct tgtcttacat caatgccttc ttggtttggt ctagtctcca 71880
gagtatattt gttacctgga agggaagaaa agggatttct tagaaaaagc aaaatatgtg 71940
gacttttaaa gttttgtaat tatcactgat attgcttaag atgaacttta aaaataaagt 72000
tctaatacac agaataactc aaatttattt gaatgttaag tgaataaaca ggataataga 72060
gtacaaacca gcaacatttt aaactatagt cgtctctacg gaattaaaag tgtctttttt 72120
tttttttttt gagacggagt cttgctctgt tgcccaggct ggagtgcggt ggtgtgatct 72180
cggctcactg caagctccgc ctcccgggtt cacgccattc tcctgcctca gcctcccaag 72240
tagctgggac tacaggcgcc caccacgaca cctggctaat tttttgtatt tttagtagag 72300
atggggtttc actgtgttag ccaggctggt ctcgatctcc tgacctcgtg atccgcccgc 72360
ctcagcctcc caaagtgctg ggattacagg catgagcaac cgcgcacccg gccttttttt 72420
tttttttttt tgagacggaa tttcgctttt attgctcagg ctggagtgca atggcgtgat 72480
ctcggctcac cccaacctcc gcctcccggg ttcaagtgat tctcctgcct ctgcctctct 72540
agtagctgga ttacaggcat gcgccaccac gcccggctaa ttttgtattt ttagtagaga 72600
tggggtttct ccatgttggt caggctggtc ttgaactccc gacctcaggt gatctgcctg 72660
cctcggcctc ccaaaatgct gggattacag gcctgagcca ccaagcccgg cctaaaagtg 72720
tcttataaaa attatacttt tggccgggtg catggctcac gcctataatc caagcacttt 72780
gggaggccga ggcaggcaga tcacctgagg tcaggagttc aagaccagcc tgacaaacat 72840
ggagaaacac cgtctctact aaaaatacaa aattagccag gcgtggtggc acatgcctgt 72900
aatcccagct acttgggagg ttgaggcagg agaatcactt gaacccagga ggcagaagtt 72960
gcggtgagcc gaaatcacgc cgagtcaaga ttgtgccatt gcactctagc ctgggcaaca 73020
agacggaaac tccgtctcaa aaaaaaaaaa aaattatact tcttggctgg gctcagtggc 73080
tcacatctgt aatcccagca ctttgggagg ccaagatggg tggatcacct gaggtgagga 73140
gttcaagacc agcctggcca acgtggtgaa aacccctctc tattaaaaat acaaaaatta 73200
gccaggcatg gtggtgggca cctgtagtct cggctactcg ggaggctgag ggatgagaat 73260
cacttgaacc caggaggcag aggttgcatt gagccaagat tgtaccactg cactcctggg 73320
caacagagtg agactctgtc tcaaaaaaat atgtctatat ctgtatctat atctccactt 73380
cttaaaatcc ctatgagata gtaggtatta tgagtgctta aaagacaaaa taagatccac 73440
atggcctcca ttcatgctta ttttaatttt caattcccta tgtactccat aattaattga 73500
acagagtcac tgagggattt ttttagaact tgccaagtta cttgtggaaa gaaatttact 73560
attttgggcc aaaccattaa atttgtgatg tgaattctca tagatgtgat ctagactggt 73620
taaaaaaagg atgcaatgct atttttataa ctctccagcg atgtatgtag tgtaccacta 73680
acagctctca tgaaatctta ataactaatt caagtagggt aaaatatgta agtcaaaaac 73740
aaatagtcaa attctctaaa taataccttg caaattaggg actgggagat gaaattccaa 73800
tgatcggtgt ctctccaaaa tggcatcttg actggaaata tcctaagatc ctggcataat 73860
aacttttatt attggttcta cttacactac taagtgagat cctgtctttc ttctgcagag 73920
ataaaactat ggtagagtat gttcttgaag tggtttaatt tttttttctt gtgtttttat 73980
aatatctgat ataggggcac tgggaaagtc cctctttcat gaaattggat gtctacagaa 74040
agccaccaga tggcgctctc aaactcagtc tttagctcag tagtgctgca cagtactaag 74100
attcctgtcc cagaatgctt aacagatcca gtgaagacag aatgtgcaag aaacagccta 74160
gcaagtaacc ttaaccttaa ctcattaacc ttttcctagt cacaaagtaa tcatatgcat 74220
tcatactcac tagaagtagc ctatcaccca agtttccagg ttgttcttgt ttttgtttaa 74280
gatagggtct tactctgttg cccaggctag actgcagtgg tgtaatcaca gctcactgca 74340
gcctcgatct cctgggctca agcagtcctt ctacctcagc ctccagagta gctgggacca 74400
caagcattta ccatcacgtc cagctatttt tttaattatt tttatttttt ttggtagaac 74460
cagggtctat gttacccagg ttggttccag gttcttaatt agttctctca ttttctcact 74520
aatgaccagt tcaagctggg cacagtggat catgcctgta atcccagcac tttgggaggc 74580
caaggcaggt gcatcacttg aggccaggag ttcaagacca gcctggcaag catggtggca 74640
tgcctatcat cccagctact caggaggctg aggcatgaga atcacttgaa accaggaggc 74700
agagactgca gtgagccaag atcacaccac tgcactccag cctgggtgac agagtgagac 74760
tctgtctgat tgattgatag ataaataaaa taatataaat aaataaataa gacctagttc 74820
tgctttactg cccagagagg tcctgagaca ttctctattc tctcactcta cctgaggggt 74880
ccagatgctc ttaggtgcat acccatagtc ctaactcctt agggaaaaaa aaaggccttt 74940
tgctggagaa tgtttgaagt tctctcgtat caaggtgaaa tttgagtatt gatgaagcct 75000
ttcttttcta ctaaatgaag attctaaaag ccacagttct agagggagtt ttgcttaagc 75060
tccttagtgc tttacactgc catctcggct cactgcaacc tccacctcca agattcaatt 75120
gattctcctg gggccagata tcttctgact gcatggcctt gaacaagtta ctttacttct 75180
ctgtggctca atctcctcaa ctgacattag tggacttaca gtacataatg agaaaaatac 75240
acgtaaaaaa agtataagac tattacttaa caaaaatgtt aaagtcttac gacaccaact 75300
gttgtttagt atgcagagaa tgagaactca gacactgcct gatggagtgc atcttgaatc 75360
aaccgctaca ggaaacaacc tagtcaatct gaagatgtcc atatcctact acatagtaat 75420
tccactcctg gatatatgta tccagagaag tctgtgtaca aggagacatg tacaacaatt 75480
ccatagtaac actgtttcat taacaggaaa atgaataaat agtggtacag tcattcaatg 75540
aatttttcaa agagcaataa aatacaaaaa ttatcagagg aatactcatc aatatggaca 75600
actcttacaa aacaaaatgt tgaatgaaaa aatacaagcg gcagagatta cacacaataa 75660
aatactattt ccatgaaacc taaaaacata taaaactata aacacggaaa tgataatcat 75720
taaagaagat agtggttatc tctcaggagc aggaaggaaa atgcaactga agtgtacacc 75780
ggatgctcaa agtgcatttg taacgttctc tttctttaag cagtgtgaca gagaatgatc 75840
tggcaaatgt ctgtgtacct acctctttgt atgacaacta catcataaat ctgtaaaaat 75900
actgaagtat ttaacataat acttaataaa taaacaacgg aaaataaaca acaaacagta 75960
gctactgctg ttacaatttg aattgtagga aggactcttg gcttctgagt gaatcattat 76020
acaaaaagat gtactcccca gagaaaaaaa tagaaattgt ggctagaagc tgagtaaatg 76080
gtccagatga ctgcccccca acacctaaga aaccatgtat ggagaacaac gtgaataagg 76140
ctgggataat aaataccaat tcagtctcta gacacatact gtcctaattt tgtgctcatt 76200
cagctgggtc atttatgtca aattaaaatg ctgctacttg tactaaaatc ttgaatccta 76260
cctttctggc tctgttgcat ccaggtgagc tccagcagtg aatggaggag cagggttgct 76320
cacctactgt gacccttcca ctctacgcct gctaggagag aacactgcct tggcatttag 76380
gaattcaggg attcttgtcc tgactccatc atgtgctaat taagcaacct tggaaaagtc 76440
tccaaatatc tccaaggggt catttatttc atctgtaaaa tagggataat atggatatat 76500
gtccaggtgc taaaagcaag gaaaggctca atccctgact ctccttcatt tcctagctgt 76560
gagacacagg caggtttctt aacctctctt acgcctcagt ttacttattc ataaatgggc 76620
ataataagag gaccaacctc acaggattgc tgtgagaatt aaaatataaa taatctgcca 76680
ggcgcggtgg ctgacacctg taatcacaac actttaggag gctgaggcag gcggatcacc 76740
tgaggtcagg agcttgagac cagcctggcc aacatggcaa aaccccgtcc tactaaaaat 76800
acaaaaatta gccaggcatg gtggtggtca cctgtagtcc cagctactcg gggaggccga 76860
ggcaggagaa tcgcttgaac ctgggaggtg gaggttgcag taagccgaaa tcaagccact 76920
gcactccagc ctgggtgaca gagcaagact ccatctcata aataaataaa taatccatgt 76980
taaggctcag catagcatct atcagacaat aattactgaa tattaacatg gggttttgtg 77040
aagcctaaga aatgtaataa tggatgcgaa gaggggttgg taaattgtaa aatccagtaa 77100
gtgctacttt atattttcat tttaacaact attttaaatt ctgtttagaa caaataaagc 77160
ttacaaactc agcactaatg tatatgaata ctgattttca agggaataaa atttattttc 77220
ctggaaaatt ctgaagccag caatatattt cttatactgg gaaggaatga gttttaagtt 77280
ttagaaacgt cttagaaacc acaggtttct ccaatagtat gttctcaggg aaaggtcaac 77340
ttgatagtta taacccatta taattttata tgttctctga aaacccaaga tcctgaaagt 77400
taggctaaca tacctatagc tagttacttg gaattattat aaacttttta aaaagccctt 77460
tcttcttgct atagcaatct tctaaaaatc ctggaagtga gagactaaag ccttttgata 77520
tatgtgaatt ttcccacaaa atttaaaccg acaattcctc tgcagctgag taagtatatg 77580
caagtacaag atatgagtta taaaagggga ctttaagtga aaacctctta aatgccatca 77640
gaaaacactc ctacacttga ttggtccagc tcttgttttc agtaaatttc tagttttcca 77700
cagacacata tcccagtaac gagaaaaaag atagtaacaa aatccatgga aaagctaagg 77760
gaacaataaa tgattacatg gactaaaata tatgaaatgt ttttacatcc atgagtctat 77820
gtgatactca aaaaacaaat tagtcaccct gaaggatgct gacgaaccaa ttcattattt 77880
tgaaaattgg taaataaatg gaatggaata aaagcattta tcctgccttt cccacagtaa 77940
ctgtaccact gactagccaa atagtagaaa aggagatttt acagcagtac tcaagttaat 78000
caaaagagat ggaataatag aattagcatt agaaaaacca aaattaggga ggtgatgcac 78060
tgagaatcaa tatttgcaac atcataaatg accagacatt gtatgcccaa tggaaaaata 78120
catcaccacc tatgaagatg tcttgtcaaa aagtcaaaca caaatttgat caagcctcta 78180
tatccaactg ccaatttgta gaaatacaga agacagagga acttgttaaa ctacattgtt 78240
aggggtgcag taaaaaaaaa aaaatctagg ctatgagaag ttcgataaaa caaacaacct 78300
gattattcaa taaataaatt ccaagagggg agtgagggga aggagtagag gaagagatga 78360
aggatgaact cttaaactaa aaagagactt aaaagacata tcagactgga gtggtggctc 78420
actttgggtg actcactttg gggtcctagc actttgggtg gtcaaggtgg gagaatcgct 78480
tgaaactagg atttcaagac aagcctggac aacatagtga gatcctgtct ctacaaaaat 78540
taaaataata ataataataa taatgaaaga catatcaaac aatcactaat atgtaatact 78600
tatttggact ctgattcaaa taaactttaa aaataatgat ttttataaga acactagaaa 78660
tttgaacact aactggatat ttgataataa tggtaacaat aatggtattt ttatgttttt 78720
aaaaatgaat tgctatgttt tagagatata tactgaaata tttatgagca aaatgatatc 78780
tagaatctct tcaaaataat acaaaagggt gaatggaaat gtggattgta cagaattggc 78840
caggggcagg gtgatgcaca catagggtca ttatagtatt ttgtgttctt ttgtgtatgt 78900
tttaaattct tcacaaaaaa aaaatggtga agggaagaaa agaggaaata agggtgggag 78960
ggaagagaga aaaaagtaat tgaacttgta ttagagtctg atttggtgtg ttattatgtt 79020
taccacagga tcaggagtca ctgaactacc ctctggttca tattccacaa ttttattggg 79080
ttctcttttt ctattctatt ccttcctact tctctatgca aaagcatcct ttgttatact 79140
caaactatcc taactcatgt tctccaaaag aaaccaggct cactccttgg tcttttcttc 79200
atgtcatggt tttgggggtc aacttaatta ctatcatgaa gaacataggc tttgagttca 79260
gactgacttg ataattgaat tccataacct atacagatag ttccactacc accctatctc 79320
tctcctctct gtactccctg caagactaaa acattagtca aatacaacaa atattagttc 79380
cttttctttt tccccttgaa tggctcttcc ttctgcttaa ggaaatctta tctatacttc 79440
aggagaagtg aaactgcatc tccgctaaga tcctggagaa ataaattctg ggcaacacta 79500
aaacatgcaa aatagaatag tgtttaaaag ctccaaatct gggcacagat cctgactcca 79560
gcacttaaaa gctgagggac ccaaggcaat ttaattatct taagtcttaa tctactcatg 79620
taagatggaa atatgagcat ctacctactg gggttactgt gagaattatt agaaattaaa 79680
taatccacgt aaagtgctta gcatagcact caataaaaat tagcaagaaa aaggaggaag 79740
aaaatggaga gatgatgatc ctggaaaatc aaagctaaaa ttacactcag tgaaatcctg 79800
ggtttgcctt gcttccccaa ctaaactttc agcaccttga gtgcaatgac actagttaca 79860
cttctttgca ttttctaatg cactttacat gtagggggac taaaaaaatt acttattaac 79920
tgagagagag aaaatttggg ctcatttata tttataatcc tatgaacttg gaataagcat 79980
actagagtaa atagcagcgg agtcaggcag atgacctgag ctcagaagtt cgagactagc 80040
ctgggcgaca tggtgaaacc ctgtctctac taaaaataca aaaactagcc aggtgtgatg 80100
gcacctgcct ttaatcccag ctacttcaga ggctgaggca ggagaatcgt ttgaacccag 80160
gagatggaag ctgtagtgag ccgagatcat gcgaccgcac tccagtctga atgacagaag 80220
gaaaccctgt ctcaaagaaa gaaaagaaaa gaaagaaatt ttctttcttt tcacttgctg 80280
ggcacagtgg ctcacaccat aatcctagca ctctgggagg ccaaggcagg caaatgacct 80340
gaggtcagga attcgagacc agcctggcca acatggtctc tacttaaaaa aaaaaaaaaa 80400
aaaattagtt aggcattgtg gtatgtgcct ataatcccag caacttgggt ggctgagaca 80460
tgataaccac ttaaacctag gaggcggagg ttgcagtgag ccaagatcat gccatgtcac 80520
ttcagcatgg gcgacaagag taagactcag tcttaaaaaa aaaaaaaaaa aaaagtcacg 80580
cacacctaaa atacagctcc accctctata ttcttgtctt caactgtgcc tttcattcta 80640
cctatacatg aaggagtacc tgttttgttg ttcccactca ctctttgacc tccttctttt 80700
atttccctcc cagctgaata aacccagttg ctcccacagt gtcggtagag ttatgtttgg 80760
agaaacagat cagtaaggaa taaccagaac taagtttttc cttttactat tctaaacctg 80820
cttgtaaaca ttcaactgct cctactttaa ttccattgag aggcaaacaa aaaaacaaaa 80880
tagggcagaa aaatggtttc tagtttatca ggaactatga aactaaaggc atatgtgaat 80940
aatgaagaga aaaagtgaaa gtggatacca actaaattca ataacatatt aaaaatatca 81000
ttcaattcac aaattagctg ggcatggtgg caggcatgta atcccagcta cttgggaggc 81060
tgaggcagaa ctgcttgaac ccgggaagtg gaggttacgg taagctgaga ttgtgccact 81120
gcactccagc cttggtgaca gagtgagact ctatctcaaa aaaatatata taatatatta 81180
ttatatatat tatattatat ataatatatt ttatattata atatatatta tatataatat 81240
attttatata atatataata tatattatat tatatataat atattttata taatatattt 81300
tatatattat atattttata taatatataa tatatattat attataatat attttatata 81360
atatataata tatattatat tataatatat tttatattat atatatattt tatatataat 81420
aatatatatc attcaacatg atcaagtggg attcatctca gggatgcaaa gatggttcaa 81480
cataaacaaa tcaggctggg cacagtggct cacgcctgta atcccagcac tttgagaggc 81540
cgaggtgggt ggatcacctg aggtcagaag tttgagatca gcctggccaa tatagcaaaa 81600
ccccgtctct actaaaaata caaaaattaa ccagatgtgg taatgggcgc tgcaatccca 81660
gcttcttgaa aggctgaggc aggagaattg cttgaacacg gaagttgcag tgagccgaga 81720
tggcaccact gtactccagc ctgggcgaaa gagtgagact ccatctcaaa caaacaaaca 81780
aacaaaaata tatacaaata tatacaaatc aataaacatg atacatcaca gggtcaggtg 81840
taatggctca tgcctgtaat cccagcactt tgggaggccg aggtgggtgg atcacttgag 81900
atcaggagtt caagaccagc cttgccaaca tggcaaaaca cagtctctac taaaaataca 81960
aaaattagcc aggcgtgtgc ctgtaatccc agctactggg gaggctgagg caagagaatc 82020
acttgaaccc gggaggcaga ggttgcagta agccgagatt gcaccattgc cctccagcct 82080
gggcaacaga gtgagactgt ctcaaaacaa aataaaacaa aaacatgata catcacagta 82140
acagaatcaa ggacaaacac cataagctga aaaaggcatt caataaaatt caacatcact 82200
tcatgataaa cgttctcaac acactgggta gagaaggaac atatgtcaaa acaataaagg 82260
ctgtatatga caaactcaga gctaacatca tatggaacag cgaaaaagtg aaagcctttg 82320
ctctaagatc tgaagaagac aagcatcccc acttttacca ctttatacag catagcactg 82380
gaagtcctag ccagagccat tagacaagac ataaagggca tccaaaatgg aaagaagtca 82440
aactgtcatt gttcgtagac aacataatct tatatttaga aaaacctgaa gactctatca 82500
aaaacacctg ttagaactga taaatgaatt cagtaaagtt gcaggataca aaagtcagca 82560
tatcaaaatc agtagcattt ctatatgcca acagtgaaca atctgaaaaa aaaaaaaaaa 82620
aaaccaagaa agcaatccca tttataatag ctacaaaaaa caaaaaacaa acacacaaca 82680
aaaaagccct ctaagaacaa actgaaccaa aaaagtaaaa gatctcaggc tgggcgcagt 82740
ggctcacacc tctaatccca gaactttggg aggccaaggt gggcagatca ctcgagctca 82800
ggagtttgag accagcctgg ccaacatgcg aaacttcatc tccactaaaa atacaaaaat 82860
cagctgggcg ttgtggtgca agcctgtaat cccatctact tgggaggctg aggcagaatt 82920
acttgaacct aggaggtgga ggttgcagtg agctgagatg gcgccactgc actccagcct 82980
gggagaaaga gcgggactcc gtctcaaaaa aaaaaaaaaa gtaaagtaaa agatctctac 83040
aatgaaaact ataaaatact aataagagaa attaaggagg gcatacacaa aaaggaaaga 83100
catcctgtgt tcatggactg gaagaactaa tattgttaaa atgtccatac tatccaaagc 83160
tatctacaga ttcaatgcaa cccctatcaa aataccaatg atattcttca cagaaataga 83220
aaaaaaaatc ctaaaatcta tatggaatca agacaaggac tttttcagaa tcctactgtc 83280
ctttcacagc taccaatcag gtattatact attaatatga tttatttaac attattatgg 83340
gcttcatatg cacaatctca tttaatattg gcatcaacct gatgtggaaa ggaactgggg 83400
cttagagaag ttaagaggct gggcatgatg gttcatgcct gtaaccctag cactttggga 83460
agctgaggca ggaggatcac ttgagcctag gagtttgaga ccagcttggg caacataggg 83520
agaccctatc tctacaaaag attaaataat tagctgagtg tggtagtgtg tgcgtgtagt 83580
ctcagctact tgggaggctg gggtaggagg atcatttgag cccaggagga ggaggttaca 83640
gagtgccaag attgcaccac tgcattccag cctgggcaac agggtgaggc cctgtctcaa 83700
aaaaaaaaaa aaaaaaaaaa aaggacaggg tctcactatg ttgcctagac tggtcttgaa 83760
cttctgggtg caaaccatcc tcctgcctca gcctcctgag aagctgcgat tacaggtgca 83820
taccaccaca tctagctttt tgtgtctctt ttgaacaagg attttaaatt tcaatgaggt 83880
caagtttatc tctaaaccta tggttcatct ctatcttcat gggtttttct gtgtgtttaa 83940
gaacatttcc tctatctgaa gataaaaaaa attctcatat attttctttt aaaagttgta 84000
gcgaaccaga ctcagtggca catgtctgta gtctatagaa agtctacata aagtcctaag 84060
aaagaactat atttttttca tttgtaatgt acctctgcct tatatcaagt ttccatatgt 84120
ttgtggatgt ttccagggcc tataatctgt tctacttgta tcttcatgca cccctgcttc 84180
aattctacta ctgtaacaac tagaacttta taataagtct tggtacctgg tagaagtagc 84240
tttccttgtt cctcaaaatt atcttagcta ttcttggcct tttactctct tattaatttt 84300
ataatgagat tatacaattc ctaaaaaaaa acttacagga ctttcaactg gcactgcatt 84360
gaatttagag aaatttgggg catatttaca tttatgttat atagagttta accatccatg 84420
tgcatgatat acctccccat ttaggcattc ttttatatcc attacatatt tcttgcaact 84480
ctttcattgg atttatttct gaatatttta cagctttgtt aaatattgca aatgaaataa 84540
tttttacccc agcattttcc tgtaattgat tattgataca agaacatggc cgggcacggt 84600
ggctaatgcc tgtaatccta acactttgag aggccaaggc gggtggatca cgaggctggg 84660
agatcgagac catgctgact aacatggtga aaccccatct ctactaaaaa tacaaaaaat 84720
tagctgggcg tggtggcacg tgcctgtaat cccaactact tggggagctg agacagggga 84780
atcgtttgaa cctgggaggc ggaggtggca gtgagccaag gtcacgccac tgtactccaa 84840
agaacgcaat tttagtgtgt ttgtcttctt tctggcaacc ttgctgaatt atttttaatg 84900
actgatctga agattttctt cagtattttt atggaggcaa tcctatcata tggaaataag 84960
gaaataggtt ttgttgtgct tcctttccat ctcttataat cacattttcc cccttgattt 85020
attgtaatga ttaatacctg taagtatagc caaacaaaag tgatagtggg catccttatt 85080
ttttcaacag aaatcctttg aaacctccat taatcatgaa gtttgacaat agcttttaat 85140
cagcttgaaa gttttttatt gttgtggttt atttttgttc atggatgagt gctgtgtttt 85200
atccaatgct cagagtctac tgtctatcac tttggactac taaaactgtg ggtcaggacc 85260
cattatattg taattaaatg catttaaagt attagcagat cagagtttct ctagaaaaaa 85320
aaaaaaacag aatataaggg aaagtgccag actacatccc atgatgtaag atagtgtttg 85380
acaaaatttt gtttcagctg cttacacaaa cacatatatg tactctgaat tgctaaataa 85440
aatgtgcttc ttactatgag ttgtatacaa aaaagagttt gaaagccact aatctatact 85500
atttctgagc atgaaaaccg actgttaggg acaaacatct tactgtaaag ttttcttaaa 85560
acagataata caatgtgtgg tttttattca ttcaaaaata cacacctttt tctggtatta 85620
ttattttaaa tcaaaatccc ttgatgagac caggcgtgat ggctcatgcc tgtaatccca 85680
gcactttgga aggctgaggc gggcagatca cttgaggtca ggagttcgag accagcctgg 85740
ctaacatggt gaaaccccat ctctactaaa aatacaaaaa ttagctgggc atggtggtgg 85800
gtgcccgtaa tcccagctac tcagaaggct gaggcaggag aatcgcttga acctgagagg 85860
cagaggttgc agtgagccaa gatcaccata ctgcactcca gcctggacga cagagcaaga 85920
ctgtctcaaa aaaaacaaaa caaaaaaaca aaacaaaaca aaaaatcccc tgatgaacaa 85980
agtggcccac actcacaaaa acatctgagg ccaaagcaat ctacataaat tagcatgaat 86040
ttctgacaag gaacctactg caacgatcac ttttgcaaag ttttcaatat gcataatatc 86100
tgagataagg gcatacaact gtcatcaaat tttcaaatgt acctgtgacc ttaaaaatac 86160
acagaacttc tactccaaaa aattcaacgt aagtttataa ttctaaaatc agaaggtaac 86220
ttgacatttg cattacttat atcacatttt ctgtactgtg tattgagtct ggctcacttg 86280
tttctcagct gtttatatta ccttgtactt tgctaactgc ctagcttttc aggctataaa 86340
aagaaatatg ccctgaaact tcacccatat ggatatgcca tgcaatgtat ctctttgccc 86400
atcattcctg aaacccttct atagaatatt ttaagattgt tcttgtggcc aggcatggtg 86460
gctcatgcct gtaatctcag cactttggga ggcgaggcag gaagaatact tgaggccagg 86520
cgttcaagac cagcctggtc aacatagtga gaccccacct ctacaggaaa aaaaaatgtt 86580
ccttgtttgt gaccagaagt aacactactc accttattca ctggatttat ttctggaaaa 86640
tgtgtaactg ctttctgatt atgctgatta taatttgtca ccagtgaagc tatttaaaaa 86700
aaggcaactc ccaaagagga gctccaacat attctgcaaa atagcagcat tgtttgtgta 86760
agtatctaac ataccaaaag gactacttta aataatcgcc aggcgcagtg gctcacgcct 86820
gtaatcccag cactttggaa ggccgaggca ggcggatcac ctgagatcag gattttgaga 86880
ccagcctgac caacatggag aaaccccgcc tctactaaaa gtacacaaat tagccgggca 86940
tggtggcggg cgcctgtaat cccaggtact caggaggctg aggcaggaga attgctggaa 87000
cctaggaggc agaggttgta gcgagctgaa atcaggccac tgcattccag cctgggtgat 87060
aagagccaga ctccgtctca ataaataaat aaataaataa ataaataaat aaccatttga 87120
atgtaatgct tttgaaaaaa caaaaaaaaa gttgttgttt tttttttgag acagggtctt 87180
actatcaccc aggctggagt gcagtgactc aattgtggct cactgcagtt gaactcactg 87240
gctcaagcca tcctcagcct cccaggtagc ttggactaca ggcacacacc accatgcctg 87300
gctaattatt ttattttctt gtaaagatag tgtctcacta tgttgcccag gctggtctca 87360
aactcctgaa ctcaagaaat cctcccacct tggtcttcta aagtgttgca gttacaggca 87420
tgagccacca tgcctggcct gatatttgtt ttttaaaact cacattccat tcttgacata 87480
ccatgtatac tgatgttctc cacaaaaatc actttttaaa aaattcagca ttatgtagac 87540
attctaagaa ccacactatt catcttggca attatgttaa tattgagtgc caagtaccac 87600
ggttctaaag aatataatat cactgcttca aagtataatt tttgaaaact cctaattctg 87660
taaagctatg ctaatttctc tgactgctgt tggatacatt aggatgatat ccaaacttaa 87720
agaaagttgt ctttatggtt ttaaaaagtt cccaaaacta atttataaaa taagcatgtt 87780
ctcataatag agaagtgaat ttttaagcat aaatttctaa gtacattaaa taatatatta 87840
tcattattct tatgtacttt tataataccc aaaacagtat tttaaaagta tagcaatctg 87900
ttaacaatga ttacctctgg tgagcatgac tggcaggaat agataaagaa tagacagact 87960
ttgtgtttct gcttttattt ttactctaaa tgcttttgta ctgtttaaat atttaaacaa 88020
gtactttttt aatgaaaaat tgaagaaaaa ggtacaatta taaagtcaag agtctccacg 88080
tttatcttat tctacttcct gttatcccca tttcttatct ccctctagga gattgtagca 88140
ctttaagagc tgggatcttg tgtgtcttct acagtgtcct atctcccaca gagctaaccc 88200
catagcagac gctatgcatc tggattagca gaagtagagg caatgaggac aagtggattc 88260
aacaatacta cagtgagcct ttacaaaaaa aagtactttg gaaggtaggc tttggaataa 88320
aagaatctat tatcttctcc atatggaatg ctggttaata tctattcaca actctaactg 88380
ctctgctcct taaacgttta gaaccctatt tgaacaggat gtaagtagaa atgtggaatt 88440
ttgaaatcta tcctgaattg taaaatatgc catgaactgt atatgccatg ggttatatac 88500
ataaatatat acataaatat attttatata tacataaaat ataatactat gcagtggcca 88560
tgggtttaaa tgaggtcaag gaaagcagct gttgacaaac ctgtcccaaa tttactgaag 88620
gggtgtttag gattccctgt agctttttcc aattgaaaga gtctccaggc atcattcatc 88680
acattcttct catgttctga atcaactgca ttcacctctc tgtctttgca actttcatcg 88740
aacaaggggc acagaaaaaa ctgtgcaaac ctaagggtac gaaacataat taaaacttga 88800
aagatgtcat tttaaaacaa tagcctttta gaaagatttt tggaacttat tagataggaa 88860
atgtaaattt tgcagaatca gaaacaaaac taccattgct actaagaaaa aaggagtata 88920
aaataagtgc tatgtaacag aagaaaaatc taatgttgaa cagaggtaaa tgcagaggag 88980
ttgatctacc ccagagatga taaacagtga aaatttaaaa aattaacacc aaaaaaggca 89040
gattatggac aatagcagtc tatttaagat ttttaatttg gtacgtctat aaaaactttg 89100
ttacatgtgg ctaatgctcc aaaaaaaaaa aaaaagagaa agaaagaaaa aggcaggtca 89160
tagtttttag gccaatctta aatttacagt tttaggtgca atttcactta gactcaggca 89220
ccttaggaca aacaagtgac ctacttttaa caggctcaat ttgaatattt ctttcttctt 89280
atgtagaaaa acaaacttga attagtagga aaaaacataa tgcagtaaaa aacctgaggt 89340
atgacatagt ttgactaagc catgattatg ggcgtttatg aaatcataag agaaaaagtc 89400
cataaaattt taataagcag tgattcaata aataacatgg tatgtaactt taacataagt 89460
aaccaatatg cctttcttga ggctattcct taataataat acctaacttt aagataccta 89520
cagctaataa attaactctt ctatatcagg ttgaatttaa ctcatgattc ctgtagtaat 89580
tcttaagaaa ctttacatcc cattttatag aataagactt gattcagatg aagctaagag 89640
cagaggctct gaataggact actttattca gtgggcctaa ggaactcttg ggtccataaa 89700
catctcagtt actacgcctc acagtccagc tgtcacagaa gccattatca ttacttgtta 89760
tttactttaa gtgcataaga aaatatgata ctatgctaag tactttacat ataccacaag 89820
gggaaactct atctatctgg agtctataat caaagatgta tgaaaacaga tgatttctgg 89880
tcacataaca tcttttaccc acctcaatac ttccaggctg ggaaaaatgt ctaatagcta 89940
cataccaaag ctggataggc ccagataaag gatcatgtta tgacacagta taataaagtt 90000
ttttaaatga tcctcttata caagtggatt tttagtttga gccaagtaac agacatcccc 90060
catcatacag agatctatta tatgaacatc tgctgcagca tttgtaaagc aagagatgaa 90120
attcagtgta tgatcccagg gaactaatta atggaagtct tctgggaata catattagcg 90180
atgattacac ctcaaagctg ctgcctaaag actgaaacat aaaataaaat aagtaggaaa 90240
cagactactt tggcccagca catctcataa acccataacc ttgccttgat aatgtttttt 90300
caataatata ataactatta ttaaaagtaa gcaaataaag ccgacagtga ctaaaactgc 90360
caaatttcat tatagatcca aaaagttgca gaattacacc ttggcatcac agaaaactat 90420
aaatattcac caaggataaa caaataacta ctgcctacca taatgctttg gactaccaga 90480
gtgtagtcca ttatttgagt caaataatga atcaaatcac tgatgcaaca aaaaaattag 90540
aaattgttag ctgtataaaa aattgaccaa aaaaaaaaaa aaaaagctga ccaaggctgg 90600
gcgcagtggc tcatgcctgt aatcccagca ctctgggagg ctgaggtggg tggatcaact 90660
gaagtcagga gttcgagacc agcctgacca acatggtgaa accccatctc tactaaaaat 90720
acaaaattag ccaggcgtgg tggcacatgc ctgtaatccc aattactcgg gaggctgagg 90780
cagaagaatt gcttgaaccc ggaaggcgaa ggttgcagtg agccaagact gcaccattgc 90840
attccagcct gggaaacaag tgcgaaactc cgtctgaaaa aaaaaaaaaa attgaccaag 90900
aaattaaatc atcctaatgg agcttgctcc agaaaacatt caaaatatcc ccacggttga 90960
ccaagaaatt aaatcggcct aatggagctt gctccagaaa acactttcaa aatatcccca 91020
cggttgaaac agcaggtcaa aacgttctgt atctactaag catccttgag aagcaaggaa 91080
atgaacgagg agagaatatt taaggtttag cattctggaa cagaagtatg tctgaaaaga 91140
gatcaatgac attatcacag gttagtcctc atcacatgct ttatatgtaa caccagttag 91200
aatggaaagg gctgctgaaa attctatacc tcaataaaat attttgtagg cctgaaggct 91260
catttaaaat atgacatcac tcattttttc tttttcagaa aacacaggga aaaacactgt 91320
ttaaaatacc ttttgttaaa ttatgaagcc atctctgtgg aaataataat tattattatt 91380
aatgtgataa gtacacaaag ttggataatt catagggtat tctgcattac ctgtctaggg 91440
caccttctag gtgttcatga gaaacatcaa aatagtaatt ggtatgctct ccactagtaa 91500
aggcatttga acttcctgca tgctcactga gaaactggct gtattcattt tctttagggt 91560
atttctttgt tcccaaaaaa agcatatgtt cacaaaaatg acttaagcca gcaatatttg 91620
gaggatccga caatgaacct gaaagagaaa acacgtatat aataaatcat tgtcctatgc 91680
tgaaagttag taagtacagg tgatttgtag ttcttgttag catgacaact ccaactatat 91740
aatcagagaa agttaagcac aagctaccta aaatgatatt atttagaaag taaatcagta 91800
gtcagaagac tggtttctag tgcctgctct tgacgctaat tagttggtaa cctcaggcag 91860
gttacttagt gtctctgaac attagtttcc ccacatgccg gagaggaaga aagagctgga 91920
ttcacagttt taagaggtaa aattttcttt ttatttattt atttatttat ttttgagacg 91980
gagtcttgct ctgtcgccca ggctggagtg cagtggcgag atcttggctc actgcaagct 92040
ccgcctcctg ggttcacgcc attctcctgc ctcagcctcc tgagtagctg ggactacagg 92100
cgcccaccac cgcgcccagc taattctttg tatttttatt agaggcgggg tttcaccgtg 92160
ttagccagga tggtctcgat ctcctgacct cgtgatccgc cagcctcggc ctcccaaagt 92220
gctgggatta taggcttgag ccaccacacc cagcaagagc tagaattttc ttgtcatgat 92280
ttcattgcag gagactatac agcacagtgt taacagcatg cgtttggggg ttaccactct 92340
actggctgtg aaacggtaag cacaatactg tacctatctt gactcaatcg cttcaatata 92400
tacccatctt acagggctgt gtgagtacaa attagatgac atatgcaaat caccctggta 92460
gtctaaggca ttatggtagg cagtagttat ttgttcaata ttaccttatt tcttcagttc 92520
taaaatgaca ctgagttaaa agtttttcaa agaggacaaa aagaaacact gtcccactaa 92580
ctgtacacat acataaaaag caactcggcc aggcacagtg gcccacaccc gtaatcccag 92640
cactttggga ggacgaggtg ggtagatcat ctgaggtcgg gagtttgaga tcagcctggc 92700
ctacatggca aaacctcgtc tctaataaaa atacaaaaat tagctgggcg tggtggcggg 92760
cgcctataat cccagctact agggaggctg aggtaggaaa attgcttgaa ccttgaaggt 92820
gggggctgca gtgagcggag attgcgccac tgcactccag cctcggcaac agagtgagac 92880
tctgtctcag agaaaaaaaa aaaaagaagc aacctgatta aagcaacatt aaatgtgtaa 92940
gaaatgtgcc catggcaggg catggtggct ctcgcctata atcccaacac tttgggaggc 93000
tgaggcgggt ggatcacctg aggtcaggag ttcgagacca gcctggccaa cgtggtgaaa 93060
cctcatctct actaaaaata cccaaattaa ctgggcatga tggtggacgc ctgtaatccc 93120
agctactcag gagggtgagg caggaagatt gcttgaacct gggaggtgga ggctgcagtc 93180
agccgagatc acaccactgc actccagccc ggacgacaac agtgaaactc cgtctcaaaa 93240
aaaaaaaaaa aaaagaaatg tgcacacaag gctgggagca gtggctcacg cctgtaatcc 93300
ctgcactttg ggaggccgag gcgggcagat cacctgaggt caggagttca agaccagcct 93360
gaccaacatg gagaaacccc atctctacta aaaatacaga attaggcggg cgtggtggca 93420
cgcgcctgta atcccagcta cttgggaggc tgaggtagga gaattgcttg aacccgggag 93480
gtggaggttg cagtgagccg agatcacacc attgcactcc agcctgggca acaagagcaa 93540
aactcccact cagaaaaaaa aaaaaagaaa aaagaaatgt gcacacaaga acctatgaaa 93600
tatggtatta ggccaggcac ggtggctcac gcctgtaatc ccagcacttt gggaggccaa 93660
ggcaggcaga tcacaaggtc aagagatcga gaccatcctg gccaacatgg tgaaacccca 93720
tctctaataa aaatacaaaa attagctggg catggaggcc catgcctgta gtcccagcta 93780
ctcaggaggc tgaggcaggt gaattgcttg aacccaggag gcagaggttg cagtgagccg 93840
agattgcgcc actgcactcc agcctggcga cagagtaaac tctgtctcaa aaaaaaaaaa 93900
agaaaaagaa aaaggaatgt tatattaaat gtaccacggt aaaaaaaaaa atcataactg 93960
ttactgtaaa gaatggcaat gaatttttgc tgctaggtca ccttaggtgg gaacctaaca 94020
agatgtcctt agaaaatgta agattatttg actgaaacaa ctgcagttta ccaatagctt 94080
tacgagggtt ctggtataaa ataaggtaca tggaaccggt aactattgag tatttaaaca 94140
cgtatggaaa gttctaataa atgtcagcat taggtcaatg aaacaaaaca aagcttaatt 94200
cacaattttc aattgtacct atgtgcacat caagtgctgc tgatgactta tccgtggtgg 94260
gatcactgat aagaagtact ttgataccat tggccagctc tagccctcga tattctcgct 94320
tgtcttcagg agacttggta atgtgatttc ctattctctt gatggctgga ttattcattt 94380
tgctgtaagt ctttttttgg aaactgaaaa gaaagagatt tttaattgtt gatttgtgac 94440
tttatattta aggacaaaca ataacgtcaa gtaaacccat ataatacatc atcagatttt 94500
tctaaatgct gaaggaaacc ttttatagcc actctacagg gtgcagtcat tcctttcctc 94560
cctcttaccc ctcattcttt ctgagaaatg ctaacaaaat tgttttcatg gagccatatt 94620
tgtatctgaa agagcacatg gatagggatt tgagataggg tgggttagac catgtgttgg 94680
cctgtatgca gaactagaat agagtttttt ttgctggttt tgtttttgtt tttttgagac 94740
agggtctcag tttgtcaccg aggcttgtat gcagtagcgt gattttggct cactgcaggc 94800
ctcaacctcc tgggctcaag tggacctcat cctcctgtct cagtcccccc aagcagctgg 94860
gaccacaggc actcaacacc acacccagtt aattttttgc attttttgta gagatgggat 94920
ttcaacttgt tacccatgct ggtcttgaac tcctgagctc aagccgtctg tccacctcag 94980
cctcccaatg tgctaggatt acaagtgtga gccactacgc cccgacctag aatagagttt 95040
tatttcacac tatttcagag gtatctgcta gaataagtga ggtgagatgt tgtgtaagaa 95100
ttattactgg gttagcccaa acactgctca aactgatgat attgaaagat agggtagatc 95160
tggaactcgc aggtctgaca tacacatgct cagaacatag ggaacaactc ccatagggaa 95220
caacccccaa tgaggaacaa tcctgtaaag ttctaatata aaataagaga ccggtgctgt 95280
cctatgacgg cagcaataac aaggacataa aacaaacaaa caaaaagttg gaaggacccc 95340
aagtattttt gtaacctgct gaagagctga atgccagagc aggaaaagtc tggcaatctt 95400
aacagaaatg tcagaaaaat tctcttgcaa agggcttcct ctgacagtct tctttcttct 95460
ctacaaaggc tagattacag tgatgggaaa ggactatcag tacacagaat catgtaggga 95520
tgttggttgc aattagtgca gtgaagggac tttccagagt ctggggtacg catgtttttt 95580
gagagtaggg tagatgtaac ttttgtctat actggagatt gcttaataga caaaaaaaaa 95640
aaaaaactga aactgctact actggcaggt taatatactg ccagtatata attaagtaaa 95700
gccaaaaaca tatctggaac tacagaggaa aaataaccct ctatgagcac atctgcttct 95760
tacctacaag acaaaactaa acagatctga ctgttcctta aagactgaca gctagattta 95820
aacgtaagta agagggggaa gaaataggca tacaattatt ttagataagt tttttctgtg 95880
ttaaaatctc tagagattga gagaggaaaa aaaatctagt ttgctttcac cattatattt 95940
tcctctctga aatcacctat aactattctt ccaatctgca taacaattga taaatcaaag 96000
gggtttgttg ctactaagac tgtcccctag atcccctaga tctttttttt ttttctctcg 96060
ccctctctta cagtgctgat agatcttctt tagaggagag gagatacgga attttaaaga 96120
tcaacatatc acactaaatg tataatactg gttttaaaaa tcacatagta ctttacaaca 96180
gatagtgcta atggtctcct atttatagta aaaatcattt cttgaaaaac aaagcataaa 96240
atataatact aacatattgg ggctatgtta gtaggtcact gttggaaaat tcaaaagcaa 96300
cttgaaactg aaaagccatt attaacaatt attttcagcc aggcacggtg gctcgcgcct 96360
gtaatctcag cactttggga ggctgagacg ggcggatcac aaggtcagga gatcaagatc 96420
atcctggcca acatggtgaa accctgtctc tactaaaaat acaaaaatta gatgggtgtg 96480
gtggcgtgca cctgtagtcc cagctacctg ggaggctgag gcaagagaat cgcttcaacc 96540
cgggaggcaa aggttgcagt gagctgaggt caagccactg cactccagcc tggcgacaga 96600
gtgagatcct gtctcaaaga aaaaaaaaaa ttattttctt tctcattagc cataagccac 96660
taaaattcaa tggcaaagaa cgatgaaaat atctgagtgc caagtctaaa aaatggtgtt 96720
tgcggtaaaa tagtaataat tctcagggtg acaggaaatg gtctatttta gactctaaag 96780
aggggtacaa ggaacctcag ataacagtcc taggccaggc acagtgactc acgcctgtaa 96840
tcctagcact tcgggagact gaggcgggca gatcatctga ggtcaggagt ctgagaccag 96900
cctggtcaat atggtgaaac cccatcttta ctaaaaatac aagaaattag ctggacatgg 96960
tggcgggtgc ctgtaatccc agctatttgg gagactgagg cagcagaatt gcttgaaccc 97020
gggaggcaga ggctgcagtg agccaagatc atgccactgc actccagcct gggcgcaaca 97080
gagcgagact ccgtctcaaa agaaaaaaaa aaaaaacagt tctggttaag acatttggca 97140
aaggacaatc tgtttagact gcagcaaatg ccttcagtgt gtacacagtt tgtttcaaaa 97200
acattaattt gagttcaata taaattaaac tatttttaaa aatgaatcca acttagcagc 97260
gacataacac taaataagcc acaaggcagc ctccattaag acacccagca agaagggcag 97320
tgttaaaacg gcagtgttaa taagaacctc cgtgtgtacc aaagagaatg cctgaactta 97380
ccccaggcaa gaatctactc cagggagaaa agaggttggt cttctggtga tagttatact 97440
caatttcctc tgtggtgctt gacctagtga gtttgactta ggtaaccaaa aattacaaat 97500
gactgaaatg taataatgat gatgatgatg atggtctaaa acttctgtcc aaaccacaac 97560
tgtgtagagg ggagatgaaa tatagctcac tttggaagta taaattaact tactatgtgg 97620
gaggttaaaa actctatata aagataacca cctcaactat aagctacact ctgtactttt 97680
tttcagggtt tcaacttccc ttatctggat tttaattttt ttagacaaaa aaaatttaaa 97740
ttgtaaaaca cagagtagga tgtaggtaga cagaacagtt ccagcccagt aagaactatt 97800
tgcaaatgat cagaatccat cacagccatg gatataactt atttggacgc tgaagttgtg 97860
ctggattaat attcctgata tggttgagaa taaagagata gatttattct acaagtcaca 97920
cataaaaatt agtccaacta ctagtgtaca aaggatcaag aaaagctaaa aatttccaag 97980
gtggcaatac tccctaatca aatatcatga cttcttgagg aagtaaaacc caaaagaaat 98040
agtagcaaca ttttcaaaca aaacctattc ctggcaatcc tattcaatac aaagtctggg 98100
tttacctata gcatatctat aaacacttcc tacaacgatt tgattctcac cttctggaga 98160
agtgaagaca atgactggga actcttcctc ctctgatgat gaattcatca acagcttgcc 98220
tgtctctgta tttatactca taagatcacc tgtctatcta cttcagatca ttagcataaa 98280
gccttcaaga gtaattttat ttatggcaaa tgttctccaa taagcaagca tactgacaat 98340
ttagatgacg agctagtgtt aactcaaatg ctttcccaga catttatatt tttaacttgt 98400
taaagttaaa attttaggag caataaaatt actttcaatt ctagggacat tgtcatacac 98460
tggaaacttc ataaggcaca gaactgtctt ttcagcactc tgataaactg gagagatatt 98520
tagtaattac aacatatatt ttgtcattct gctcagaatt taaaacaatg actataatca 98580
cagaagataa aagctggaag agccactaga tgttatctag tcctttgcag ttcagggact 98640
ggctacactg gcttctcctg ggagcttgtt aaaaatgcaa aatatcagaa cccaccagag 98700
acctactgag ccataatctg cattttaaca agatccccag gtgggctaga tcccctcatc 98760
ttacatacaa ggaaactgaa cctcaaggag tttaataaat cagttgccca agatcataca 98820
acaaaaagtt ataatcaaac tcagtcttaa ctcccagtcc tgtgtccagc ctcccctcca 98880
catccaaaca aacacttaag gaagtctatt aaggtccagg catcacacta tgcaaatggt 98940
tacaaaaatg agtacaccag agcccttgtt ttgaaggaac ctagactagt gatgaagtca 99000
gatataaaaa caggtcatta tctttttttt ctactttttg agacaaggtt tcactctgtc 99060
acccaggcac aatcttggtt cactatagcc tcaacatccc gggctcaggt gatcctccca 99120
cctcagcctc cccagtagct gggactatag gcactcacca ccgtacccag ctaatttttt 99180
gttttgtttt atagacaggg tttcgccatg ctggccagac tggtctcgaa ctcctggctt 99240
caagcaatcc acccgccttg gcctcccaaa gttctgggac tacaggcatg ggccaccgtg 99300
cctagtctct aggtaatttt tttgtagaga tggggttttg ccatgttgtc caggctggtc 99360
tttcaaactc ctgggcgcaa gagactgccg ccttggcctc ccaaagtgct gggattacag 99420
gcatgagaca ctacacccag cccaggtcat tatcttttaa gtgtggtgac aaaactttcc 99480
ctggggcact atgggagtaa ccctgtctag gagggggagt agaagcacac caaataaaag 99540
gcaaggggac aatcaatcaa agtagaaggg aattcgtgag cgaagcctga caatgaggaa 99600
aaagcatgag gaatacagtg gaaaaacaag aagcgtggtg ctaatgaact acaacaggaa 99660
ctccaggtca agatcatgga ctaagacatg taaaggccta aatttcaggt ttgcaaaaac 99720
aaatccccaa atataaggtc atttccaaat tccacaccaa gagtaaattt tactctgcaa 99780
agaatctttt attcaggtga cctttcagtc tttctcatct ctctttaaag tgtttcatga 99840
tcaatatagt agcagattgg gaaattttac tttgcttttt gcttcctact cacctccatt 99900
ggtctctcac aaatttaaag tcagacctga tttagaaata atgacctata aacctgaaca 99960
ggtgagtgat cacgttaata gtgaattaca aaccaaaacc tcccttctgt ggaatccatc 100020
cacccactcc caaaaccata ggtggaagtc aaggcctata gcactcatgc atgtggagga 100080
ggagcccaga gagaaaaggc atggaatcaa aaccacacca cataacattc ccagttcctt 100140
gcagaaggta gaggtaaata agcagagggt gacaagatag tggtggtatg agcccaagga 100200
tgcctcaaca tattaatgct taagaccata taccccaagt actattaata atacatgaac 100260
taaaaaataa caacatgtct tagggaacca cctacccaaa gctcttcagt tataggaaca 100320
ggtacaccta acttcataca ccagctagat cataaagttg ggtacaaaat aaaatcctgt 100380
taaacttatt cacccctgat gttttgcctt tgtgccaggc agaatatcag ctatcgtaac 100440
tctatgtcct ggccctctgg aaaggagttt atgatcaaca aataaaccct taaatgaagt 100500
agggtggttg caatttaaag aaattctaaa gtagtatcat tctagagaga agtgcttttg 100560
caaatgttgt tttagtttga tgcatgccta tcaatggttg ttcattccaa aagtgacaag 100620
cttaacacag aacactttat agaaacgtta taaagtttaa gcaagtgaat aaaacctctg 100680
ttttagacct tgtgccacag aggtaactat agaaagtttc ttgtagtttg ggtaccaagg 100740
gtgtttcaca agtatccttc ttccacatgg agttttcagg gctgttagga aatgtaagat 100800
ataggccggg tgccgtgggt cacgcctgta atcccaacac tttgggaggc cgaggtgggc 100860
agatcacctg aggtcaagag tttgagacca gcctggccaa catggtgaaa ccctgtctct 100920
actgaaaata caaaaattag ccaagcatga tggcgcatgc ctgtaatccc agctacttgg 100980
gaggctgagg caggagaatc acttgaaccc aggaagcgga ggttgcagtg agccaagatt 101040
gcgccactgc actccagcct gggtgacaga gcgagacacc atctcaaaaa aaaaaaaaaa 101100
aagatagaat accattgtac aaatctaatc ctaacaatca ctctcatttt tttaaagtgt 101160
cataggcttt ataaactgtt catttaatcc ttccattctc cttaagtggt gggtcgtaac 101220
agctgttcgc agtcatcctc ttcaactcca aatgagaaac aaaagctcag tcagtgcttg 101280
catactaaag tataaccaaa aagaaaaaaa agaaaagata agtggttaag tgacttgccc 101340
aagggcaagc tgattgtggt aaagctggaa ctacagagaa agaggaagcg tgggaagggg 101400
agggacagag gtggtgctga aggaaatcat acactggttt attctccagc tcctgtttat 101460
caccctcaca gtccttccct agccccattc ttcttccctt acaatcccaa ataacaaaag 101520
ccaggaaaca aaaattaaac taggtcaaaa taaatgctcc aaggaaagtt ttcagttttt 101580
cactccttct tctgcatata aaacacaggt agtaggggga gtctgcaatg cttctaatct 101640
accaaaggaa tcccttagct aagaaccata atacatggca ggattaaatg tataacaggt 101700
ttaaagcaag agagtatatg tacagaactg actcatgcat gaaaggaaac ataaaaacaa 101760
gtactacagt agacatgaat gggtttaagt atactatttt ttaaaagtat acattaaacc 101820
atataatcag aaaaaagaga agaaaatcat aatcatcttg gtagctgcag aaaagtactt 101880
aaattcaaca accattcacg tattttttta aagtcagcaa actggaatta aatttatcca 101940
ttttaaaaag aatatcatag aaactatggt aattatcata ctcaatggta tactcaatag 102000
tttacattac aattaatgta aacaatctcc cgccctcccc caccaacacg atgccactaa 102060
cacttcttct atttcaacat tgtattggta gttctaacca gtccaaataa accaagaaaa 102120
agaaacaaaa cacatagaga tgggaaagga aagaggaaaa aaattaacat gattctcaag 102180
caatatgatt atgtatatag aaaatcccaa gaatctactg acaacttcca cttctgggta 102240
ggatatagca gctcatggca aactaattca gcttcaacaa ctagaaaagg ccatgataaa 102300
ttacaaaaat cacatttgca aagatgttgg aaagttgtgc aagcaacaag gattggataa 102360
actagttcca gaaaggtgag caacctctga ggtgagctga taatcatcat tcattttctt 102420
atctgggaat atctgctaat tctgagtgca gactgaagat catgcttgcc ctaagcaaag 102480
ggactgtact ggacgaagag gtattattga ggcttcagtg gtggcatgaa gttggtgtga 102540
cagattaaag tcccaggagg tcccaaattt gcaactaact ttcccaacag aagattttct 102600
gagatactac tatgtattta ccagaatggc taaaaagaaa aagagggcaa ataccaagtg 102660
ttagagaagt taacaaactc tcacacactg ctacagacat gcaatttggt atgggtacaa 102720
ccattttcaa aaactggcac tatacactaa agctcacact atgacctaac aatgctagta 102780
acagataaat aacccaaata aatgcattta tatgttcata gcaacactat tcaaactagc 102840
ccaaaccaga aatgatcaaa tgacaatcaa cagtgaaatg ataaattgta catgcacaca 102900
atggaaagta tacagcaata aaaaggaata atatagaaca aagcacaata aacatgaatc 102960
tcactaatat agggttgagt gaaagaggct agacacaaaa gagtaaatac tgtaaccttc 103020
catttataca aagcagaaga gcaagtaaaa tcaagctatg ctttcatccc aggatagtca 103080
ttacccttgg gagtgggagt agtgattgga tttggaaagg agaatgaagg gggctttggg 103140
gttccaggtc atgtgctggt tatacaggta cgttcagttt gtaaaaatgc attaagttga 103200
acactgccat ctttcaatta aaagttctta aaaatagagc ttttcaaaat atcataaaac 103260
acagtaaaat aaaaggaagg gggagataca tgaattactg atattacaaa tatcaaccgc 103320
caaaaatcat cctggaaata gtaccacttg aatactggaa agcagctacc cataccaaag 103380
aggtgctaaa ctaagaaggc tcactaagga acagtagtac aattaacata attaaacctt 103440
ctcaggtgtg tctcgagaca attatttcaa ttatttctgt tgatgctatc ttggccacaa 103500
gaaataggag gcccatgtaa gtacagcatc agtcaactaa gttaattcag tatgctaaat 103560
cttttaactc aggactttac tcattacata ttatttgaaa actagctttt agtaattact 103620
ttagactcta gttaagatct aaaaataaaa cttgacactt ccagtaacta atagctaagt 103680
ataacataga taaaagcaaa atgattaaac tttgaataaa ttctcccctc aacaattttc 103740
aatatttata ccaaacaact tctaattcag gatttgaatg agaaaaatta tttttaaaac 103800
aaaattaaaa gaataccata taacgttaac atttcttcaa actcaactaa ttttacatat 103860
gggcaacctc ccacagatgc ttcatgaacc cagaacagca agggcagctt aaagacacct 103920
gtcatggttt tcttcttctt cttctttttt gtaagatagg gtctcactcc cagcgcccag 103980
gctggagtgc agtggcacta tcatggctca tgacttcctg ggctcaggtg atcctcccac 104040
ctcagcctcc tgcgcagctg ggactacagg catacgccac catgcccggc taattttttg 104100
tatttttttt tttttttagt agagacaggg ttttaccatg tttcccagga tgactcgaac 104160
tcctgggctc aagcaatctg cccgcttcgg cctcccaaaa tgctgggatt acaggcataa 104220
gccactgcac ctggcctggt ttctaaagat caattcaaat cacaggctgg gaagagttat 104280
cgacctgtaa gtaattctct caagatagcc ttctaaaaaa agcaaaccat aaatatccct 104340
tatcaaaaat ggttgggatc agatgtattt cagattttcc tggattttta aatatttgca 104400
ttacatttac cgatagagca tctactttga gcatcatgtt ggtgctcaaa aaatttagaa 104460
ttttggagca tttctgattt tgaattttga atttttcaga tttggaatgc tcaacctgta 104520
ttctgtacag acagttcagt tccactcata tgtacagaag atgatctgcc caactcagag 104580
aatgctgcct caggaaactt gacctgtact ttgccttagg tgtgattctt cactgtctgc 104640
actgatctac tgaccacttc cagcccgtgt ttggtaatta cataccctca cctttaccag 104700
atgacctctc tgctcctgag gcactggata ctactggact attctggtca aaccagactt 104760
tctggcctca tctagcttgt ttttttaaaa aacagttctc cctatgttgc gcaggctgaa 104820
cttgaacacc caggcccaag cgatcctccc atctcagcct cccaagtagg atggtgtgcc 104880
atcgtgccca tcttggcctc atctagtttt gacaatcaag agtcagatga ggccaggcgc 104940
gatggctcat gcctgtaatc ccagcacttt gggaggccaa ggcaggccga tcacctgagg 105000
tcgggagttc aagaccagcc tgaccaacgt cgagaaactc catctctact aaaaacacca 105060
aaattagcca ggcgtggtgt cagactcctg taatcccagc tactcgggag gctgagcagg 105120
agaatcgctt gaacccagga ggcagaggtt gcggtgaacc aagattgtgc cactgcactc 105180
caacctgggc aacaagagca aaactccatc tcaaaaaaaa aaaaaaaaaa aaaaaaaaaa 105240
agagtcagat gaaatgtata catggctcat taaattgaca ggggcaccta tagttgcatc 105300
acactcagac aacctttcac agagtttctc ctgtaaaata tgtttatata aacttcacca 105360
aggcatagtt cctggtacac agtagtcatc catgaatggt aacttgatta ttataaaatc 105420
atcatcatta ttatggtcgc agtggctaag gtgcaaatga accacctcca taaccaccat 105480
gttattacca gcattcttaa cacatactta tgtgaattcc agatgttatt tgtcatctct 105540
ggaggtcaaa aacattcatt aggtaggctg ataataggat tatactttat aagactatta 105600
ggaaaccagg taaataatta tccctttata attgctatag atgagccagt gtttatagta 105660
agtagttctc aatgggtgac tattcccact caccaaccct cgctgaggac atatggcaat 105720
gactagaggc attttttatt gtcacaactg aaggtgctaa tggcacatag tgagtagagg 105780
ccagggatgc cactaagcaa cctacactgt atgggatatc tcccacaaga aagaattata 105840
tgatccacac ctgtaatccc agcactttgg gaggctgagg cgggcggatc acatgaggtc 105900
aggagttaga aaccagcctg gccaacatat tgaaacactg tctctactaa aaatacaaaa 105960
atcagctggg catggtggca caaacctgta atcccagcta ctcgggaggc tgaggcacaa 106020
gaatcagttg aatctgggag gcggatgttg tgtgagccaa gactgtgcca ctgcactcca 106080
gcctgggcaa cagagtgaga ctgtgtctta aaaaaaaaaa agattttggc acaaaatatc 106140
aatagtgcta aggatgaaag accctagctt atagttctga ttttattacc cccaaaataa 106200
tggatgaaaa tcaatgcaac caattctatc cacttaaatc caagagacag cctcattaaa 106260
gtacagaacg cttctataat taaactccca tctatgtatc tacatatctc tcaatcaatc 106320
aatcaatcaa tcaacatcta agccacaaaa gatttacata aagagaggag aaaaactgat 106380
aatgaaagag aaagagagaa ggaaacttta caggaacctt gtcctagagt aaggaatcta 106440
gtacgtaagt gaaggggtta agcttagaaa gaagcaaagc cagtatgtca gaacagatgc 106500
ttactgaata ctcagtcagt atcttgtggc acatatactt gtttagggaa taaatctatt 106560
atatattata catatatata atatattcaa taaacccctt atatagatcc tattagtccc 106620
attctttact ggatggggaa attaaagctc agaaaagtta tttggttgga accacatagg 106680
tataaaatag aagagctagg attcaaaccc aagtctttag acacaaaatg accatgctgt 106740
ttaacactcc accaagttaa aagttcatct caacactttg ggaggccgag gcaggcagat 106800
cacctgaggt caggagttca agaccagcct gaacaacatg gagaaacccc gtctccacta 106860
aaaatacaaa attagctggg catggtggcg catacctgca atcccagcta ctcgggaggc 106920
tgaggcagga gaatctcttg aacctgagaa gtggaggtcg cagcaagctg agatcacgcc 106980
attgcacgtc agcctgggcc atctcaaaaa ctaagtaaat aagtaaataa ataaataaac 107040
tgacctatat taaaataaaa aatttctgtg caaaggacac agtgaaaagg caacctgtgg 107100
agtgggaaaa aatatctgat aaggggttaa tatccagaat atattaaaat ctgcaacaaa 107160
acaacaaaaa atttctaaat gggcaaaggt cttaaaaagc cacttctcca aagaagatat 107220
acaaatggcc aataagcata taaaaagatg ctcaacatca ctaatcatta gaaaagtaca 107280
aatcaaaact aaaatgaagt accacttcat accaattagg ataattattt taaaaacact 107340
gtgaatacaa aaaattagcc gggtgtggtg gcaggtgcct gtagtcccag ccaatcggga 107400
ggctgagaca ggagaatggt gtgaacccag gaggcggagc ttgcagtgag cagagatagc 107460
gccactacac tccagcctgg gcaacagagc tagactccgt ctcaaaaaaa aaaaaaaaaa 107520
aaaaaaatcg tggccaacat ggtgaaaccc cgtttctact aaaaatataa aaattagctg 107580
ggcgtggtgg catgcgcctg tagtcccagc tactcaggag gctgaggcag gagaatggca 107640
tgaacctgga aggcagagct tgcagtgagc cgagatcgtg ccattgcact ccagcctggc 107700
aacagagcga gactcggtct caaaaaataa tattaaaaaa taaaatagaa taaataataa 107760
gtgcaggtga ggatgtagaa aaactggaac ctgtgcacac ttgctagtgg gaatacaaaa 107820
tggtacagca gtatggaaaa ctgtatggca gttcctcaga aaattagaaa tagaattacc 107880
atgtgatcta acaattccac ttctggatac atatacaaaa gaaatgaaac caaagactca 107940
aacagatatt tgtacagcta tgttcatagc agcagtactg gcaacagcca aaaggtggaa 108000
gtcacgccag tgtccactga tggatgaatc agtaaacaaa acgtagttta tacaataaaa 108060
tttattcagc cttaaaaagg aaaaaaattc tggcacatgc taccatatga acctagaaga 108120
catactaaat gaaataagct ggctgggtgc agtggctcat gcctgcaatc ccagcacttt 108180
gggaggctaa ggcaggtgga tcaggaggtc aagagatcga gaccatcctg gccaacacgg 108240
tgaaacccca tctctactaa aaatacaaaa attagctggg agtggtggtg cacacctata 108300
gtcccagata ctcaggaggc tgaggcagga gaattgcttg aacccgggag gcagaggttg 108360
cagttaggca agactgcacc actgcactcc agcctggtga cagagcaaga ctccatctca 108420
aaaaaaaaaa aaaaaaaaga aaagaaatca gctaatcaca aaaggacaaa tactacacaa 108480
ctccacttat ataaggtacc tagagtagtc aaattcacag acagaaaaca gacaggtggt 108540
tgcaagggtc tgaagggagg ggaaatggtg agttattatt tgatgggtac agagtttcag 108600
tttggtgaga tgaaaaaagt tctagagatg gatgatggtg atggttacac aacaacgtgg 108660
atgtatttac tgccaccgaa caatacactt aaaaatgctc aaaatgggcc aggtgcagtg 108720
gctcatgctt gttaccccag tactttggaa agccaaggta ggaagatcac ttgaggccag 108780
gagtttgaga ccagtctggg caacatagtc agaccccatc tatacacaca cacaaaaagg 108840
tcaaaatgat aaattttatg ttacatattc tttatcacaa taataaaacc aaccatgatg 108900
ggggggtggg gtgagaaaca atcaatattc taacaaacta acctaacaac tataaatgag 108960
taacataatc acattgaagg agtgtaaaag aaaagaactt aactatggaa agctggattt 109020
tgactggata caataaagcc taagagaaaa agaactacat gcaaatattg tgacctattt 109080
taaaaatgtt tctcacaggt gagctagcaa ttatgaaact atgtgtacac tagaattgag 109140
gaaataagaa agtaaattgt agaaaatgaa atccagtgtt ctcattgttg gaaaaagatg 109200
ttataaataa gaaatgggag aaggctacaa ttgtggtact aagttggaat caaaggtatc 109260
aatacaaact tatattttta ttatatacaa aaagacatag aaatataaaa acgtgtatat 109320
gtggctgtcc accagaggca ctagactggc cggccactag aagcaatgac acctcagcaa 109380
caatgagcac atctagtgcc cagatcttgg tctgcgaaga ttattttctg ctcaggtgag 109440
ttggctcacg cctgtaatcc caggactttg ggaggctgag gcgagcagat cacctcaggt 109500
caggagtttg agaccagtgt ggctaacatg gtgaactccc atctctacta aaaatacaaa 109560
aattagccag gcatggtggc acatgcctgt aatcccagct acctggaggc tgaggcagga 109620
gaattgcttg aacctgggag gcagagattg caatgagccg agatcacacc attgtactcc 109680
agcctgggtg acagagtaag actcagtctc aaaaaaaaaa aaaaaaaaaa attattttcc 109740
aataaaagga actaagtgat tgattcaagg gctagggcaa gaaaaatatg agtctagaac 109800
agaagtaaaa caatactcta aaaaagatgg ggcttattca aaatacacag gagccaaaat 109860
gaagaaactc ctaagggtcc aagccataat aatttgagca acaaaacaaa gaacgatagt 109920
actgaatttt cagccgtaag ttaagataaa tgtatgttca tagcaatata aataaataag 109980
aaaaagaaaa agcaggctgg gcatggtggc tcacacctgt aatcccagca ctttgggagg 110040
ctgaggaagg cggatcacct gaagtcagga gttctagacc aggttggcca acatggtgaa 110100
atcctgtctc tattaaaaat acaaaaatta gccaggtgtg gtggcacacg cctgtagtcc 110160
caggtactca ggaagctgag gcaggagaat tgcttgaact tgggaggcag aggttgcagt 110220
gagccaagac tatgccactg cactccagcc tgggcaacag agtgagactc tgtctcaaaa 110280
aaaaaaaaaa agaataccaa tctttctcaa attcttccaa aaaagaagag agaatacctc 110340
caaactcatt ttatgaaatc agtattaccc tgatactaaa gccagacaag gacattacaa 110400
gaaaagaaaa ctacaggcca ataaccttga tgaatataga caaacattta gccagactaa 110460
gaaaaaaaga gagacaacac aaaatcagag atgaaaaagg agacattaca actgataccg 110520
caaaaattca aaagatcatt agtggctatt atgagcaact atatgccaat aaattgaaaa 110580
atctagaaaa aatggataaa ttcctagaca catacaacct accaagattg aaccatgaag 110640
aaatccaaaa cctgaacaca ccaacaagta acaagattga agccataata aaaagtctcc 110700
tagcaaagaa aagcccagga cccatggctt tcctgctgaa ttctactaaa tatttaaaga 110760
actaatacca attctactca aattattcca aaaaatagac aaggaaggaa taactccaaa 110820
ctcattctat gaggccagta ttaccctgat accaaaacta cacaaagaca catcaaaaaa 110880
ggaaaactac aggccaatat cactgatgaa tattgatgca aataccctca acaaaatact 110940
agcaaaccaa attcaacagc acgttaaaag gatcattcac cttgatcaag tgagatttat 111000
cccggcaatg caaggatggt tcaacatcaa cacacttatt gcaaatcaat aaatgtgata 111060
taccacatta acagaatgaa gggggaacaa ccccacacaa tcatctcaat agatgcagaa 111120
aaaaggctaa gtatagtggc tcatgcctgt catcccagca ttttgggagg ctggggtggg 111180
aggatggctt gaattcaaga gctcaagact agcctgggca acacagcaag ataccgtctc 111240
tacaaaaaca gctgggcatg atggcacatg cctatattcc caagtactca gggatgctaa 111300
ggtagaaagg tcccttgagc ccaagagttt aggtctgcag tgagccatga ttgcaccact 111360
gcactccaat caggctgaca agaatgcaac agtgtcttaa aaaaaaaaaa aaaaagatgg 111420
ccaggtgcag tggctcatgc ctgtaatccc aacactttga gaggccaagg caggcggatc 111480
acttgaagtc aggagttcga gaccagtctg accaacatgg gaaaaccccg tctctactaa 111540
aaatacaaaa ttagccaagc gtggtggcac atgcctgtaa tcccagctac tcgggaggct 111600
gaggcagaag aattgcttga acctgggagg cagaggttgc agtgagccaa gatctcacca 111660
ctgtactctt gcactctagc ctgagcaaca agagcaaaat tccatctcaa aaaaaaaaca 111720
aacagaaaaa tacctcttca cagatagctt tcagatgcca catgcaggag ctgtgaaaaa 111780
aggggggaaa aatagagcag aaaaataatt tgacaaaatt caacagcctt tcacggtaaa 111840
aactctcaac aaattaggta tacaaggaat acctccattg tgtacttcag tacaataaga 111900
gccatggaag acaagcaaac aatgaacgta ttcagcagtg aaaagctgaa agcctttcct 111960
ctataatcag gaacaagcat gacaaggatg cccactctca tttctattca tcaatgataa 112020
ttttctgatt ctgatggtat tgtgatgata tagaaaatca cactaaagag gccgggcaca 112080
gtggctcacg cctgtaatcc caacactttg ggagctgaga tggggggatc accagaggcc 112140
aggagttcaa gaccagcctg accaacatgg tgaaatccca tctctactaa aaatacagaa 112200
ttagccaggc atggtggcac atacctgtaa tcccagctac ttgggaaact gaggcagcag 112260
aatcacttga acccaggagg cggaggttgc ggtgagccgt gatagtgcca ttgcactcca 112320
gcctgggcaa caagaaaaaa actttgtctc aaaaaaaaaa aaaaaaagaa aatcacacta 112380
aagtatccaa tggtcaaatt actgtaattg gttcaggaaa aaaattattt gcactatatt 112440
gtacaataaa aacttttcag tatgagattg tttcaaaaat acagtatatc tctttcatga 112500
caaaaatgct gatcaaacta ggaataaaag agaatttcct caacctgaaa aagggactct 112560
atgaataagc tactgctagc atcgtactta atggtgacag ataatgtttt taccattaag 112620
atcaagaaga tgacaaggat ttccactctt gccacttcta ttcaacactg tcctggaagt 112680
tctaaacaga ccaattaggc aagaaaatga aataaacggc gtacaaatta gaaagaaaaa 112740
agtaggccgg gcgcggtggc tcacgcctgt aatcccagca ctttgggagg ccgaggcggg 112800
tggatcacga ggtcaggaga tcgagaccat cctggctaac aaggtgaaac cccgtctcta 112860
ctaaaaatac aaaaaattag cggggcgcgg tggcgggcgc ctgtagtccc agctactcgg 112920
gaggctgagg caggagaatg gcgtgaaccc gggaagcaga gcttgcagtg agccgagatt 112980
gcaccactgc agtccgcagt ccggcctggg tgacagagcg agactccgtc tcaaaaaaaa 113040
aaaaaaaaaa aaagaaagaa aaaagtaaaa ttagctccat ttgcagaaga catgatcttg 113100
aatataaaaa tatcaattgt atttccatac agtagcaatg aacaaactaa aaataagaaa 113160
acaattaata atagcatcaa aaaataaaa tactgggtggg cacagtggct catgcctgta 113220
atcctagcac tttgagaggc tgaggcgggt ggatgacctg aggtcaggag ttcgagacca 113280
gcctaaccaa catggtgaaa ccctgtctct acaaaaaata cgaaaatcag gctgggtgca 113340
gtggctcacc cctataatcc cagcactttg ggaggccaag gcaggtgatc acctgaggtc 113400
aggagttcga gaccagcctg gccaacatgg tgaaaccctg tctctactaa aactacaaaa 113460
attagccggg cgtggtggtg ggtacctgta atcccagcta ctcgggaggc tgaggcagga 113520
gaatcgcttg gacccaggag gcggaggttg cagtgagctg agatcgcgcc actgcactcc 113580
agcctgggca acagagcgaa agtctgtctc aaaaacaaaa acaaaaatta gctgaacacg 113640
gtggtgggcg cctgtaatcc cagctactag ggaggctgag acaggagaat tgcttgaacc 113700
tgggaggtgg aggttgcagt gagccaagat cttgccattg cactccacct gggctacaga 113760
gtgagactcc gcctcaaaaa aaaaaaaaaa aaacttatgc ataaatttaa caaaagaaat 113820
gtaaaactta aactttaaat ggaaagactc ccatgttcat ggatgggaag acagtatttt 113880
taagataaca gtaatcccca aatagattaa aatcccacct aagcctcctt ttttgcagaa 113940
attgacaaat tgatcctaaa acttgtttgg aaatgcagag gacccagaat ggtcaaaaaa 114000
gtcttgagaa agaagaacaa agctggaaga ctcaaactgc ctgacttcaa aacaattgta 114060
agcaaaatag tgtggtactg gcatcaggat aaacatacag attaatgggc caggcacggc 114120
ggctcacacc tgtaatccca gcactttggg aggccgaggg gggtggatca cgaggtcagg 114180
agatcgagaa caacctggat aacatggtgc aaccccatct ctactaaaaa tacaaaaaat 114240
tagccaggct tggtggcggg cgcctgtagt cccagctact caggaggctg aggcaggaga 114300
atggtgtgaa cctgagaggc ggagcttgca gtaagccaag atagtgccac tgcagtccag 114360
cctgggagaa agactccatc tcaaaaaaaa ccatacagat taatggaaca gaacagaaag 114420
ctcagagtta aaccctcacc ttttgatcaa tttatttttg aaaaggatga tggccgggca 114480
tgttgtaatc ccagcacttt gggaggccaa ggtaggcaga tcacttgatg ccaggagttc 114540
gagactagcc tggcgaagat ggtgaaacac catttctact aaaaatacaa aaattatctg 114600
ggcatggtgg ccaacacctg tagtcccagc tactcaggag gctgaggcat aagaattgtt 114660
taaacctggg aggcggaggt tgcagtcact gcactacaac ctgggcaaca gagcaagatt 114720
ccacctcaaa aaaaaaaaaa aaaaaaggat gcaaaacaat tcaatgagga aggactagtc 114780
ttttcaacaa atggtactgg atatccacat gtaaaaagat gaagttggac tctcacctca 114840
caccatacat aaaaactaat tcaaaataga tcaaagactt ccatgtaaca gctaaataat 114900
taaacaatac aacctttttt ttgagatagt gttttgctct tgttgcccag gctggagtgc 114960
aaaggcgtga tctcagctca ccgcaacgtc cacctcccgg gttcaagcga ttctcctgtc 115020
tcagtctcct gagtagctgg gattacaggt gcctgtcact acgccaggct aatttttggt 115080
atttttagta cagacagggt ttcaccatgt tagccaggct ggtctcaaac tcctgacctc 115140
aggtgatccg cccacctcgg cctcccaaag tgctaggatt acaggtgtga gccaccatgc 115200
ctggccaata atacaaatct taaaagaaaa cttaaatctt tgtgaccttg gattgggcag 115260
tggcttctta gatatgacat caaaagcaca accagttttt taaaaagaca aattagactt 115320
caacaaaatt taacactttc tgcttcaaag aacaccatca agaaagtgaa aagacaacct 115380
atagaatggc ataaaatatt tgtaaatcat atatctgata agggacttgt atagagaata 115440
tattttttca tttgtatttt tttgaagatg gagtttcact ctcgtcaccc aggctggagt 115500
gcagtggcac gatcttgcct cactgcaacc tctgcctcaa gggttcaagc gattctcctg 115560
cctcagtctc ccgagtagct ggggttacag gcgtgtacca ccatgcccgg ctaatttttg 115620
tattattagt agagatgggg tttcaccttg ttggtcaggc tggtcctgaa ctcctgactg 115680
caggtgatcc acctgcctca tcctcccaaa gtgctgggat tacaggcatg agccaacacg 115740
cccggcctag agaatatatt ttaaaactct tacaacttta ataaaaaaaa aaaaaaaccc 115800
aattaacaaa tagaaaaagg atctgagtag acatttctct aaagaagata tataaatggc 115860
caagaagcat atgaaaatat actcactggt catcaggaaa atgaaagtca aaattccaat 115920
gagacaccac ttcataccca ccaggatggc ttataacaaa agtacatata gcaagtgttg 115980
tcaaggatat agagaaattg gaattttcac acactgctgg tggaaatgca aaatagtgcc 116040
gctgctttga aaaacaacct ggtagttcct gaaaaggtta aacacaagag ttagcatatg 116100
acctagcaat tccgctccta tgcatatccc caagagaaac gaaaataagt gttcacatga 116160
aaacttgtac acgaatgccc aaagcagtat tattcattat atctcaagac tggaaataat 116220
ccaaatgtcc atcaactaat gaatggataa atacaatctg gtctatccat acaatgcaat 116280
actatgagta ctaaaaagga atgaagtact gacacatacc tcaatatgaa tgaactttga 116340
aaacattatg ttaagtgaaa gccagacaca aaagaccaca tattgtttga tgtcatttgt 116400
atgaatggtc cagaacaggc aaatccatag aaagaaagat gagtggctgc catgggctca 116460
gggaagagtg gaatagagtg taactgccaa agggtataat gtttcttttt gaggtgacga 116520
aaatgtttta aaatgattgt ggtgatggtt gcacaactct gcaaatacac taaaacccat 116580
gaatggtata tttttttttt tttttttttt gagacagggt atcactgtca cccaggctgg 116640
agtgcaatgg tgtaatcatg gctcattgca gctttgactt ccccggctca agcaatcctc 116700
ccgcctcagc ctctggaata gttgagatta ctggcacgtg cctccacact cggctaattt 116760
tttatttttt gtagagatag ggtcccacta tgttgcacag gctagtttcc aactcttgga 116820
ctcaagcaat cctcctgcct cagtctggga gtcacaggat ttacttattt aaaaactcaa 116880
ttgtttaagg gaaagagtta aactctagtg tgcttcattg atttcctctg ccccttttgc 116940
tgttttatga caattcctaa tttgttcatt aatatttcta gaaagatgct atgttgttgc 117000
catataataa tatcgataat agtttggctg gttgactgta agtacaaata acatattcat 117060
tttccttaaa agtctgttag tttaaccgtg tatctgactg gccagatagt cttgtctatt 117120
tggcttctgg gaacggtgtc ttcccagtgt caacgttcct atctgcctta tttatgtgac 117180
ctgactgacc agaaatccta atgtagccat cttgctccaa aaggttcttg gtcttcccta 117240
ccttgttaca ttacactcat cgtcttcttg acttactgta cactgtttgc tctcttttct 117300
ccctctcttc ttggtgagca actcaagaga tgcctcaaag gttgagagaa gtgagctggg 117360
catggtggct cacacctgta atcccagcac ttcgggaggc cctagcacgt ggatcacctg 117420
aggacaggag ttcaagacga gcctgttcaa caaggggaaa cctcgtctct actaaaaata 117480
caaaaattag ccaggcatgg tggtgcgcac ctgtagtctc agctactcag gaggctgagg 117540
cagaagaatc acttgaaccc aggaggcgga ggttgcagtg agccaagatg gagccactgc 117600
actccagcct aggcgacaga gcgagactcc gtctcaaaaa aaaaaaaaga ttgagagaag 117660
tgacttgtta ctccttttat gcatgctcac agagctcagg ctgatagctc cacttacttt 117720
cctagaagca aagctaacat gctcttccac acccaccacc ttcactccat cctcacctgg 117780
gttctagaca ataaggcgaa ctcaggccgg gtgtggtggc tcaggctggg cgtggtggct 117840
cacacctgta atcccagaac tttgggaggc caaggtggat ggatcacctg aggtcaggag 117900
ttcgagacca gcttggccaa catggtgaaa ccctgtcttt ctactaaaaa aatacaaaca 117960
ttagcgggca cggtggtggg cgcctgtaat cccagctatt caggaggctg aggcaggaga 118020
atcacttcaa cctgggaggc ggaggttgca gtaagccgag atggcaccac tgcactccag 118080
cctgggcaac aagagtgaaa ctccgtctaa aaaaaaaata aataagtaaa taaataaata 118140
agtaaggcga actcagcagt agcacagctg tctaaagtag acttcctaag agctgtggga 118200
cttttcattt ctctgtcaac ctcctatagt tgccataagg atcaaataag atgatgtgat 118260
aatcttcgta aactttaaat gaggcaaagg gtagtcctgc aaggatcaaa taagatgatg 118320
taagctgggc gtggcagctc acgcctgtaa tcccagccct ttgggaggcc gaggcgggcg 118380
gatcacctga ggtcaggagt tcaagaccat cctggctaac atggtgaaac cccatctcta 118440
ataaaaatac aaaaaaaaaa aaaaattagc tgggcgtggt ggtgggtgcc tgtagtccca 118500
gctacttggg aggctgaggc aggagaatgg cttgaacacg ggaggcggag cttgcagtga 118560
gcggagatcg cgccactgca ctccagcctg ggtgacagag cgagactccg tcaccaaaaa 118620
aaaataaaat aaaataagat gatgtgataa tcttcgtaaa ctttaaatga ggcaaagggt 118680
agtcctgatt acacaatcat ttctgtactt tcggagcaat ttacatgtgt attttgtttc 118740
atttttatta ttaacaaaaa cctccagtat gtctgaaaac tactaagact aacttcctcc 118800
aatcactgta tagaacggat cacaaatata atgtttacaa ggccaaaaaa gtaatgtaaa 118860
taagcaaagc aggctgtaag aaggaatgca tgccccttct gaaagacaaa tcttccctag 118920
ctcaagctaa ttgctgccag gcaggaatgt gggcccactg ttaccagatt atcccttttt 118980
cccaaaagga actgaactct caggatttct aagtgttgat tcccatttaa aaaataacaa 119040
ctgcaaaaca tatccacatg ccacatccca gcctactggt ctacagtgac ctcatttata 119100
gtcggtcata ctctctaaaa taaaagcaac atcaagtgag gagttagtga tatgtaagca 119160
caagaaagga aaaagaacta tgcacctgta gaaggcaacg ttcagaaacc aaaacttggt 119220
aggattaata aagatatttg aagctactta gtctggtggc taggaaaaag actcatgcaa 119280
actaaccaat tcaagataaa aagaataacc aagtgtcaag tcagagtgac ttttccacta 119340
catcacactg gctcccaaat ggatagacta tagagagaat aaggcatttt tttcaactgg 119400
atggcagttt aaagtagtct ggagtacaaa cttagaacag gtgtcaatct tactgatatc 119460
ttcctggaac agaagctgaa ttcaagaata ttttaccctg gaccaaatgt tatccaacac 119520
tcccagccta tattggccat cagcagagca tacagaaacc acagagtagc ccaacaatgg 119580
gaagggagaa actttcacca actcaagaaa gaaaaaaaag aaggaaaatc aggtgactaa 119640
tgtttgtttt cattatttct tgttttaaaa gcaaaatttc aaatttactt cctccataaa 119700
aagaaaagca ggctgggtgt ggtggctcac gtctgtaatc ccagcacttt gggaggccaa 119760
ggcgggtgga tcatctgaag tcaggagttc aagaccagcc tggtcaacat ggtgaaaccc 119820
tggctgggca cagtggctca cgcctgtaat cccagcactt tgggaggccg aggtggacag 119880
atcaactgaa gtcaggcgtt cgagaccagc ctggccaaca tggtgaaacc acgtctctac 119940
taaaagtaca aaaattagcc gggcgtggtg gcaggcgcct gtaatcccag ctacttggga 120000
ggctgagaca ggagaattgc ttgaacccgg gacgcggagg ttgcagtgag ccaagaccac 120060
atcattgcac tccagcctgg gcaacaagag caaaactcca cctcgggggg gaaaaaacca 120120
tggtgaaacc cccatctcta ctaaaaatac aaaaattagc tgggtgtgtg ggtgcatgcc 120180
tgtagttcca gctccttagg aggctgaagc aggagaatca cttgaatctg ggatgcggag 120240
tttgcagtga gccaagattg caccattgca ctccagcctg ggctacagag tgagactctg 120300
tctcaaaaag aaaaaaaaaa aaaggctggg catggtggct caaacctgta attccagcac 120360
tttgggaggc tgaggcgggc agataacaag gtcaagagat tgagaccatc ctggccaaca 120420
tggtgaaacc ccatctctac taaaaatata aaaattagct gggtgtggtg gcacgcgcct 120480
gtagtcccaa ctacttggga ggctgaggca gagaatcgct tcaacctggg gcagggggca 120540
gaggttgcag tgagccggga tcacgccact gcactctagc tgggcagctg agcaagactc 120600
cgtctcaaaa aaaaaagaaa gaaaaaaaga aaagaaaagc aaatctttaa gttcaattaa 120660
aaaaatctta aagttcaatt ttaaagggag gggaaaaaag aagccatatg tcttttttgt 120720
caaagtctca caaaggatat ccctgaaagc ttgctgcttt aaccatatgc tagataacct 120780
gtaaaataac ttaattccca agtacttaca atactagaat tctagagcta gatggaggga 120840
gtggaagcca ttcaggtcat ctctctcact tttgtagtct tattctacaa aggtgtagat 120900
tcacactagg acccagatct atacttttct gtttggaaat caccagaaga tctacaaaaa 120960
gttatctccc atgtagtctt tacatgaaag cttgttccaa tatacaacta ctcttgaatg 121020
aaggagcaag ccaagaaaga aaaaaatttg agaaccagga aacagatcca ttagaggaga 121080
gaagcaaagg gaaattccag gcaaccattc tgtagcaagc ctggagaaaa ctagttcaaa 121140
ctacagcaag aaggaaaaag tgtcctgaag gatagtctcc aaggagaaaa tatggaacca 121200
atagatcatc taatagattt gatcatataa gaaactgtac taggccgggc aaggtaactt 121260
acacctgtaa tcccagcact ttaggaggca gaggccggca gatcacttga ggtcaggagt 121320
tggagaccag cctggccaac atgacgaaac cctgtctctc tactaaaaaa acacaaaaat 121380
tagtgggcat ggtggcacac gcctgtagtc ccagctactc aggaggctga ggcaggagaa 121440
tgcttgaaca cgagagacag gggttgcagt aagccgagac tgcgccactg cattccagcc 121500
tgggcgataa agcgagactg tctcaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 121560
aaactgaaac tgtactaaat gtccatagga ccataagatc aagtgggaag aatcagaaat 121620
atgtaaacag ttgggtgtga tggctcatac ctgtaatcct tgctacttgg gaggctaagc 121680
agaacgatca cttgaggcca ggagttcaag accaacctct gcaacacagt aagaccccat 121740
ctctaaaaaa ataaaatagc tgggcataac aaagcaacag aaaatttcta aaattaagag 121800
atcaagaaat aacagtagcc aggtgtgctg gctcatgcct gtaatcccag cactttggga 121860
ggctgaggtg ggttgatcac ctgaggtcag gagtttgaga ccagcctggc caacatggtg 121920
aaaccccatc tctactaaaa atataaaaaa attagccggc gtggtagcgg gcgcctgtaa 121980
ttccagctac tcaggaggat gaaacagaat tgcttgaacc caggaggcgg aggctgcagt 122040
gggtcaagat ctcgccactg cactccagcc tgggtgacag agcgagactc tgtctcagaa 122100
aaaaaaaaaa aaaaagaaaa aattagctgg gcatggtggt gggcacctgt aattgcagct 122160
actcaggagg ctaaggaatg agaattgctt gaactcagga gacagaggtt gcagtgagca 122220
ccactgcact ccagcctggg caacacagcg agactccatc tcaaaaaaaa aaaaaaaaga 122280
aaagaaagaa agaaatggta tttgttcttt tctcaagacc ctattctccc caaaatctct 122340
ttggctagaa cttcagacta tttcacttcc gatatcatct tccacctctc taactcccaa 122400
cctctccaac tatgtttcct tcttatacct aaatgtgagc attgtcaagg ctgccccctc 122460
ccccaccgca tctaaacata gattattttt cctatatcca tccatgtccg tgtcttcaac 122520
tctatcttca gatttaggaa atcagctcag gccttcttac caagttccag tcatatttca 122580
aatgcttcct gcagatctcc ccttggctat ccagtaatca cttcagactc aatatcatca 122640
actaccatgt atattctttc taaaaaatct ccctgtttta acgtccccat ctctactaaa 122700
gatatcgtgc tacctctagg aagaagacaa tataatggcc aacccctgag gttcttgagt 122760
gagcccagtt gaattcatat ctcgctaagc cacttactag ctgtgtatcc ttgagcaagt 122820
taacctcttt gagcttcaat atccttatct gaaagaaaaa aaaaaaagac taccaccacc 122880
actctcccac ataattaccg tggccattaa acaagacaac atatgtagcc aggtgcagtg 122940
gctcacgcct ataatcccag tactttggga ggccaaggta ggtggactgc ttgaaccgag 123000
gagtttaaga ccagtctggg caacatgacg aaatcctgtc tctacaaaaa aaaaaaaaaa 123060
ctccaaaaaa ttagccaggt atggtggtat gtgccaagtt ccagctacta gccccagcta 123120
cccaggaaac aggggagtac tgagatggca ggagaatcac ctgagcccag agaggtcaag 123180
gctgcagtga gccctgatca cgccactgca ctccacctag gagtgacaga gtcagactct 123240
atctctctct ctctctctct ctctctcaca cacacacaca cacacacaca caacataggc 123300
aaagctccta ataaaatgca taaactggag gttatgatga taagtgaaat acgccaggca 123360
cagaatgaca gacatcacat gttcttactt atttgtggga tctaaaaatc aaaacaattg 123420
aattcatgga gatagagagt agaaggatgg ttactagagg ctgggaaggg tagtgggtgg 123480
ttgagggcag gtggggatgg ttaatgggta ccaaagaaaa aaaaaaagac ctaggatttg 123540
atagcacaac agggtgacta taatcaataa taattgcaca tttcaaaata actaaaagag 123600
tttaattgga ttgtttgtaa caaagaataa atgcttgagg ggatgactac cccccaccca 123660
aaaaagcata acatacaata agcattcaat aaatattagc tactaaatct tttatggtta 123720
acatcgtcag cagcagcaag agcgttacag ctgaatccac ttagacccca gcacaggtca 123780
gacttgttct aggctctgga tccatttcct atccttaagc aaaacaaata agaaacccca 123840
gtagtaggtc agtattcatc aatgtggttc tgatctttca attgaactag tccctgagct 123900
accaatctac aaactagatt tcattccctt gggtatttaa ggaccgctca gtcctgtcca 123960
ctaccctacg ggacctgagg gtaacagcca gcccaaaagt gcctacaaga ctgtgcccac 124020
tgctgtaccc aagcagtggt tcgttctctt ggtatgcctt tggaaaacct gccacctgcc 124080
aggcaggaca aacatctcac ctagctgaac ttcttcccag agctgggtct ccccgacaag 124140
agttttccct accctggtgg cccctcctat tctagactca aagtcactca cgctcaagac 124200
ctcaggcttc tctgactccc acatcgtccc tacatcaatc aataactaaa ctctgctgaa 124260
tccttcttgc acatgtcttc tgcagacact cttctttcct tgtcatttgt actaccttct 124320
ttatccctca tcagtctctg cctgcgtttc aaacagtgct tacaaaaaac agccagatta 124380
attagccaaa aatacagctt tcatatgtca ctgaattgct caaaaagcat ctgaggttct 124440
ctatatccaa taagacaaaa tttcactttc ctttgctgat attgtgaatt tccctacttt 124500
tgaacctttg ctaataccat tccttctatc taaaatattt tctcttctgg ttgtggtggc 124560
ttatgcctgt aatcacagca ctttgggagg cagaggcagg aagattgctt gaagccagga 124620
gttagagatc agtatggaca acatagcaag atcctgcctc tacaaaattt taaaaattag 124680
ccaggcatgg tggcacaagt ctgtagtcct agctacagga ggctgaggtg agaggatcac 124740
ttgagcccag gagttcatgg ctgcagtgag ttttgatcgt gccactgcac tccagcctgg 124800
gtgacagtga gatcctgtct ccaaaaaata aaatattttc tcaactctcc ttctttgttc 124860
atccaattca atatttcctt caaagactct tgttcaaatc ctatgttctc tgcaaagctc 124920
tcgccattct tactagcagc agggtgttct cagcagaggg aaaacaatgc aaatgatctg 124980
aggtaagagc aagtcaagca agtagaccag tgtgactcac attgttgagt aggaagttta 125040
ttactcaaac ctgaaatttg agggagaggt tctggctaga gaaatacatt tgggagttgt 125100
cagaatatag attaggccta tcttacagaa tttttgcaat gatcaatatg ataccagggc 125160
atacttgcat gactgccatt cagcagtgaa ctcatacatc tttatgaatg atgttaaatg 125220
aaagttaaat gaaaaaaagt aactcagaaa agtttgcaat aaaggtcaaa agctataaaa 125280
ctaaaataaa caataaaact aaacaatata ctgcttaaga agtattcaaa tgtgataaaa 125340
ctataaagaa aactaaggaa attgttatcc aaatatagaa taatacagcc gggcacggtg 125400
gcccacgcct gtaatcccag cactttggga ggctgaggtg ggtggatcac ctgaggtcag 125460
gggtttaaga ccagcctggg caacatggtg aaaccccatg tctactaaaa atacaaaaat 125520
tagccgggcg tggtggcacg cgcctgtagt cccagctact tgggaggctg aggcaggaga 125580
atcgcctgaa gctgggagat ggaggttgca gtgagccgag attgcgccac tgtactctag 125640
cctgggcaac aagggcagac tctttctcaa aaaaaaaaat aataataata ataataacaa 125700
tacctttaca ggggaggagc agagttataa ttggacaggt gagcacagac aggttcaaag 125760
ttgctggaaa tgtttgtttc ctaaagtaga tggtacacag gtgttttttt cttctttgtc 125820
ctatatactc tggcatgtac aatattaaat aagagagtaa aaaaataaga ggcaatatat 125880
aaacatcaaa tagatgtttc acaatgtttt taaaaattat taagttgaga aaaaggaaga 125940
gtaattgtgt gacctgtata acaaggccta aaaagaataa aggatttagt catataaaga 126000
aaagacagaa agggcatctc ggaattggga atgaataggt catgtaaaag gagacagcaa 126060
attggtaagt ctgactggag aagagttagg ctgaaaagtt gggctaagcc agccaacaga 126120
atgcttacat gctgggtcaa gaagtttgaa cttgatcttg aaatagcaca gaaccactgg 126180
tgatatttag gcgagacaac agcttgggaa aagtggttta ttaatctgat gtgatgataa 126240
gaatcaacta gtagggagac agaaggctgg aaaagtcagt tctaaaacct gttcaggtga 126300
gataatctca ctgatgtaag atgttacgaa gatgatcatg gcctgaacta ggatggtgat 126360
gagggtcaag atcactccaa gaaactttag gaatctttag gatgaaagac tcaggaggat 126420
tgggtcttgg gaataggcaa tgagggaaaa agattcaaag attttcaagc taaactgaaa 126480
actctcattt tcaagctaaa ctgaaaaaat gataacaaca tagcaaaact aaggactcta 126540
cagaaaaaaa ttgttttgaa aaaagaccat taatcaatta agttatgcag taaatatgta 126600
ctgagtacct actatgtgtc aggcagagtg tgatgccttc ccttcagaaa gcagattgtg 126660
agtcctactt taaaaagtta ttccgagcgc agtggctcac gcctgtaatc cgagcacttt 126720
gggaggccaa ggtgggcaga tcacctgaag tcaggagttc aagaccagcc tggccatcat 126780
ggtgaaatcc cagctctact aaaaatacaa aaattagttg tgtgtggtgg cacacacctg 126840
taatcccagc tactctggag gctgaggcag aagaatcact tgaacccggg aggtggaagt 126900
tgcagtgagc caagatcgtg tcactctcca gcctgggcaa cagaacaaga ctcagtctca 126960
aaaaaaaaaa aagttacact tttaataggc aaaaagaaac taaacagttc tttgagaaga 127020
ataaaataat tttgtgagtc ccacttttta aaaaagtaat cagtatggtg ataaaatact 127080
aagttgtttt tcaaaattaa ttgaaagaaa caacaaaaca taaaagtgga ggtgtctagt 127140
aaaaggtcag aggtaaagca gtttactgtt tattactgaa agctccttga ggacaagatt 127200
tatggttatc tttgcattta catagtactg gacacagtgt gtgatcacta cagagtagac 127260
attcaaataa tgtctaaact aacagaacag tgaaaacaga ctgaagacaa gccaaatgag 127320
aaggctccag aacctaacat atctatagag gatctacccc tgtggaggga aagtgctcta 127380
gccttgtcat cctggctaaa aggtagcctg atcctctggc tacatgttta ggagatataa 127440
aataccaaca gaaaaacaga aaggaaacca gtgtttatga aacatatact gggcctggca 127500
cagtggctca tgcctgtaac cccagcactt tgggaggcag ggggattgct tcagcccagg 127560
agtttgagac caccctgggc aacatgacaa aaccccatct ctaccaaaaa tacaaaaatt 127620
agttgtgggg tggtggcatg agcctgcagt cccagctact caggaggctg aggtgggagg 127680
atcacttgaa cccaagagat gaaggctgca gtgagctgag atcacatcac tgcactccag 127740
cctgggcgac agagtgaaac cctgtctcga actttaaaaa aaagaaaaca tactgaactt 127800
tttctcccct accctgggga gactgtcccc aaccctaact tctttttttt taagacacag 127860
tctcgctcag tcacccaggc tggagtgcag tggcacgatc tcagctcact gcacctccgc 127920
ctcctgggtt caagcgattc tcctgcctca gcctcccggg ttcaagcgat tctcctgcct 127980
cagcctcatg agtagctggg attacaagcg cccaccacca cgcccggcta atttttttgt 128040
atttttagta gagatggggt ttcaccatgt tgcccaggct ggtcttgaac tcctgacctc 128100
aagtgatccc cccgacctcg ccctgtcaaa gtgttgggat tacaggcatg agccacccca 128160
cctggcctca accctaactt tcaagacagg cagaatgtgt ggattaggtt aaagaaactt 128220
caggggtacc ctgttctttt acactattaa aaggcatttt tctgattgac agtagtttaa 128280
aagcttgaat gttgatagtc ccaactgaca taccactgtt tcctaagcct atggaacagg 128340
gaaatgcaaa gtagggctgc ctgcaaagtg agatgactga tgcccaggtg gctcaagttg 128400
ttgccaggga atgggctatt atctccaggt gccccagtag ttcagataat tccacacagt 128460
cccaagttat aaaatacagc tgaacacaga acaaggaggt tactctgtaa atggattgat 128520
cttacataaa tgctttaaga ccatctctgt ctcaagaggt tagaccagaa aatgaatgcc 128580
caaataccag cagaggcagt acttgtgttt gcaaatacat aacagttaag agctcaggct 128640
ccgatgccag atagacatgg gttaaatccc agctcttcca gcaaatagct gtgtggctat 128700
ctctgttaag acattcttcc gactatgtac agtagggctg attgcattcc ttaagatgag 128760
aatatgtggt agcttcagat gttagtgctt tccttcctaa aactatgccc taatcccttt 128820
ggttggacct gtattttcct caaaagcctc actcactgat tttagggtag agctttctag 128880
ctggctcatc tcattcatta tgctttgaat ctctcaaatt tccatctttg agaaaaacat 128940
atcttaaaac tctggatggt cgaccggact ttataactac agagaaaatg taattttaac 129000
tagaacatac aaaccactta gttatacaaa acttaaaatt ggtgctttct ttaaaaatca 129060
caaatgctat atgctggaga cagtatggag acagctaagc ctagcctatc agtaatgaat 129120
ttagaacaaa agcagactga cacaaaagag gacaaaagga ggcatcctgc ctgtgcttac 129180
cacctgattc tctaagttca ggggtctttt gcacattata ggagattttg tgatgtagtg 129240
gaaaacagag gagaacaagt tccatagagt cttcatttgt attagcttat cttagtgccc 129300
cactgctacc atcctaatac aggcagtaaa gactaagtcc caacaggttt ctctgcctta 129360
ggactctctc cacttcctga catcctgcac acagccacca aactactttc ctaaaccccc 129420
agttccatca gataactttc cagctcaaaa actttaatgg ctccctacct actacgcatt 129480
gggaaaatcc caactaaaat tcaagaccca ggccagcatt accctactgt tataatacag 129540
gacttgtgtc cttctaatta ccccaaggaa attctataaa gcttcaacca gactaatcgg 129600
ctcactgctc ccccaacaca aactgcccgc atatctaggc tggattacgc ccacgccagc 129660
cctcactcca acctttctgc cattccttcc aaagccagcc taccccccca tctctggccc 129720
cacacactac ccatactacc catcttctga ccctcatcaa cacacccttg tagctatctc 129780
aattcccgtg gcatcccaat ccgctccaat caagtcaaag ttccttggaa ggcagacaca 129840
tactttacat agagggtcca gcacagtgcc ctgcacttag taggtgctgg ctattactga 129900
ttaaaaccag caggggttca aataacgttt ctagggaggg caagataata ctgcctatct 129960
tcttccattc gtagtaaaaa gtattcagcg ttcactacaa gcctgaaaac gcctagcgta 130020
cgcagatcaa cggtgcagtg gagagggcac gccgccggat ggcaaaaaac ctgtgtccta 130080
atcctggttt tgccacaatc tcggcagttg ctgggaaatc aagccatctc tcggagctcc 130140
ggttgtctcc gacgtaaaac aagggagctg ctgtagagga tcccgaggta tcttttggct 130200
cctaaatgtc ccccgatagg aaaccccaca ctcgtcttcc aaggcgggag gcaggcaaac 130260
ttttttccca gcgcgggcat tccacacggt cctggaaact cagtgcccgg agaaaagggc 130320
agggctgagg gcaaggtgag aagcggtccc cccgaagtcg ccggattcct ttaccccacg 130380
gctcgagtcc gcctcaggtt cccggccccc tcacaggcgg actccgggag agtgacgcgg 130440
cgcggctccc aggtgcaggc cgggtgactc tgtccgcccc tgcgcccccc gcactagtgg 130500
cacacgcccc gtggccccgg gtagcggccc cggcccctca ccagcccaag ggacgtcccc 130560
gactctggac caggcctctg gctcccgcct ggcgagccgc tcttccgggc cctggggcgg 130620
cctcagcgcg gcagtacggg ccccaggccg gcgggacggg cggcgtgagg ggcagcggtg 130680
gcggctttaa gtgctgaatc accactttgc aacccgagcg ccaaaccgct gtgacccacg 130740
gggcccgagg gcccgggcgc tccattccgc ttacccacac aggcgctccg gaggcggcag 130800
gcgggcgccg aggactgagc ggaaggtgct gggcagtgcg gggtgcagaa gccacgctag 130860
ccggtaccgc attagccagc gcagtcgccg ggatcaccgc aaacgcttcc tgcttgcgct 130920
tcgagccggc cctgcgcact gcgcatgctc tagccgcggc gccgcgcggt ctcccgggag 130980
ctgcggagag gtagctgctc cgggttttgc ctggagccgg atggggccag gacgcaggcg 131040
aggacggtga agaccgcgga cagccgaggc ctgagcaggc ggactggagg gacaggggtt 131100
tggccggggc gctttaggag ccagcaccca gactttccca ggcggggagg gtcccagcta 131160
gatcccaccg gccgcacttg tgagggtggc gacgtgtgtc tgactgtgag ggggcgaggg 131220
gccgctcata aatccagtga gggcacacgg ggctatgttt tctgaggcac ccccactgac 131280
tagcgtgccg gaccctgcag gtgttggaac aaggttcgtg gtcgcggcgg cctcacgtaa 131340
aatgaatccg cggccggcgc gcggcccgcg ggctccctct agagcgcggt ggggacgtgg 131400
accgcgaagg cacagatgcg ctggagacaa ggctcgagtg cggcgtgggg gaaattcaga 131460
cgcaggggtc agcgatagag aggccaggac ccagggggac gtttccatcc ttggggaagt 131520
tagcagctct cttaagagga aggtgcggat gggacgggcg ccgcagcaaa acaaggcttc 131580
aaatccagag tagaaactac tactcttgtt agtactccgt actaggaata ctactaatgt 131640
cagtcggctt cagtggttac tttttaaaca aagaattttc tccccagtgg ctttatgggg 131700
ctttgcaccc acgaacaact taatagttgt tatgaatcta actttccata ataccttatg 131760
ggagtcttta aggaagaatg ctattaggga tggtgacttg ccagcttaag tacttttagg 131820
tttctgattg acagacgatc aagactgcag ccaaaaacgg tgtccttagt ccaagtattg 131880
ctttaaatgg gaattcagta ggagggttcc ttaatcaagt tgatgcttta tcgaagacgt 131940
acccacgatc tttttaacag tgcggtttca cctaaaggca aacctttgga tgaaaacagg 132000
taaaaattat ggcaagcctc ctagtggtgt ttgcaagtgc atggttttaa gaacctttaa 132060
attaccagtc ccaatgcaga gtagtcaatc accttgaagc agctcctctg agagtgttaa 132120
tgcagccttt ctgaatacat ttatgctcca ttaatggaat ctataaatgg gctggggtag 132180
attcagactt ggagggcttg aaacatatac aatttggggg acctctttga gacataaaat 132240
acaaattata aatacaaaat tagcaatcag aagattacta tagagagaaa atacatcaca 132300
acaaatactc gagacttgga gattagtacc cttttttttc ctgagatctc tttaggctac 132360
ttattagaaa tgcttacata aaaatgcttc cagatggccg ggcgtggtgg ctcatgcctg 132420
taatcccagc actttgggag gccaagccgg gcggataacg agatcaggag ttcgagacca 132480
gcctgaccaa aatggtgaaa ccccgtgtct actaaaaata caaaaattag ccaggcgtgg 132540
tggcatgcgt ctgtaatccc agctactgag gaggctgagg caggagaatc acttgaaccc 132600
tggaggcgga ggttgcagtg agccaagatc acgccactgc actccagcct gggcaacaaa 132660
atgagactct atgtctcaaa aaaaaaaaaa atgcttccag acaacaactg gctttccctc 132720
tacaactccc agtttggagc ctgtgcaagt gagcagccct gaagtttaaa cttcattagc 132780
ttcatgataa atttccctct ggttggagta gatgtcctgg ataagaaaga ttaatattaa 132840
tatagtctca taactatcat ctcaataaag aggaggtgga tggaaagaaa agtaacaatt 132900
ttgagagtct actctgtgtc tggcattgta catggctcct tcattagtca tcatgaccac 132960
cctctgtaat ggatatttcc tcacctacca gagatgagga agctgtggca gagagaggtt 133020
gagtaacttg ccaacaattg gacaaaaaga gtggagttgt aatttgaacc caggactgtc 133080
ttattccaaa acctgtgatt tgctcatctc atttatgact tgacaaatat ttgttgaatg 133140
ccttacagat accaggtatt ataaaggtgc tgggaataca gcagtggaca aaaaagacaa 133200
aagtttctgt cttgatggag attaccttct agctgtgtgg gaaaaacaga taataatcaa 133260
gtgagtaagt gatatgaagg aacaggtggt caggaaaggc ctctttgagg aagtgacatt 133320
caggcagacc ccagaatgat gagaaaaagt aggaaatgtg aaggtctagg gtgtaatctt 133380
tcccactctc actcaaacaa gtataaggtg taaaccacct agacaacttt tttttctttt 133440
ctttcttttt ttttttttga tggagtctcg ctctgtcacc aggctggagt gcagtggcgc 133500
gatctcggct cactgcaacc tccacctccc ggattcatgt gattctcctg cctcagcctc 133560
cagagtagct gggactactg gcgcacgcca ccatgcccag ctaatttttg tatttttagt 133620
agagacggga tttcaccatg ctggccagga tggtctcgat ctcttgacct catgatcccc 133680
cctacctcag cttcccaaag cgctgggatt ataggcgtga gcctccatgc ccggcctaca 133740
acttttttag aagcagaaaa agtaatgtgc tgaagtcatt ctttgcctta gataggaaaa 133800
ctagacacat attcaaatat aagtattctg atatactgga agccaaatat tagtgtttat 133860
ctgtgctgta agttccttcc attagcccag atagaataga gttcaccata gagctgagtc 133920
aggagttcct aatgattgaa agaaaaaaac ttaaaaagga agcccagtgg tgacagattg 133980
gcgtttattt cctaattggt aatgttcaga aaaataatat cttttgtccc cccagatgtt 134040
ttagcagagg aaagaaaagg aatttttttt ttttttttga ggcgaagttt tgctcttgtc 134100
acccaggctg gagtgcaatg gcatgatctt ggctcagcgc aacctccgcc tcccaggttc 134160
aagcaaatct cctgcctcag cctcccaagt agctgggatt acaggcatgc gccaccacac 134220
ccagctaatt tttgtgtttt tagtagagac gggtttctcc atgttgatca ggctggtctc 134280
caactcccaa cctcaggtga cccgcctgtc ttggcctccc aaagtgctgg gattacaggc 134340
gtgagccacc gcgcccgggc aagaattttt tttttttttt tttttaaata ggccgggtgc 134400
agtggctcac acctgtaatc ccagctcttt gggaggatga ggcaggtgaa tcacttgagg 134460
tgaggagttt gagaccagcc tggctaacat ggtgaaaccc tgtctctaca gaaaaattaa 134520
aaaattagct gggcatgatg gtgtgcacct gtgtcccagc tacttgggag gctgaggcac 134580
aagaatcgct tgaacctggg aggcagaggt tgcagtgagc tgagatcgtg ccactgcact 134640
ccggcctagg ttacagagtg agaccctgtc tcgaaaataa aacttaaaat gtcttttgag 134700
actgggcaca gtgactcacg cctgtaatcc cagcactttg ggaggccaaa gtgggcggat 134760
cacctgaggt tgggagttcg agaccagcct gaccaacagg gagaaaaccc gtctctacta 134820
aaaatacaaa attaactgga catggtggcg catgcctgta atcccagcac tcggaaggct 134880
gaggcgggag aattgcttga acccgggagg cggaggttgc ggtgagccga gatgaagcca 134940
ttgcactcca gcctggacaa cgagtgaaac tccatctcaa aaaaaaaaaa tgtcttttga 135000
actaatctag cctattgtta tatattcaaa atcaaaatct gaattctgaa tccaccaact 135060
caaaattttg tacaaatagc taaagagaaa gcatacctag gtagttaata acaaaggaaa 135120
atttaaagtt agggccagac gcggtggctt atgcctgtaa tcccaacaat ttgggaggca 135180
gaggtgagag ggtgcttgag ctcaggagtt caagaccagc tttggctaca tagtcaggcc 135240
ttgtctatat aaaaaaataa aaactaaatt aggcagcagg tgcagtagct catgcctgta 135300
atgctagcac tttgggaggc caaagcagga ggatcacttg agcttaggag ttcgagacca 135360
gcctgggcaa catagacctc atctctattt aaaataaaga taaaatcaaa acaaaacaaa 135420
aaattagccg ggcatggtgg tgcatgcctg cggtcccaac tactctggat gttgaggtgt 135480
gaggatggct tgagcccagg aggtggaagt tgcagtgagc caaaatcatg ccactgcact 135540
ccagcctggg caagactgcc agaccctgtc tcaaacaaca attcttacaa gaccttaaac 135600
atttttcata aacatcattt ttatttgtat tattttttaa ggctcttgag caatgccaat 135660
tctttaatga acagctgagg aacattggtt ttagccatca attttctttt tctcaaaaga 135720
aaaatgtctc aaaagacatt ttttttttaa ttgagacaga gtctcactct gtcacccagg 135780
ctggagtgca atggtgtggt cttggctcac tgcaacctct acctcccagg ttcaagcgat 135840
tctcctgcct cagcctcctg agtagctagg attataggca cctgccacca cgctcagcta 135900
atttttgtat ttttagtaga gacagggttt caccatgttg gccaggctgg tctcaaactc 135960
ctgacttcag gtgatccacc caccgcagcc tcccgaagtg ctggggttac aggcatgagc 136020
cactgcaccc ggcctttttt atctttttaa aacggggtct ccctctgtgg cccaggctgg 136080
agtgcagtgg agtgatctca gctcactgca acctctgcct cccgggttca agcgattctc 136140
ctgcctcagc ctcccaagta gctaggacta caggcatgag ccaccacgcc ctgctgattt 136200
ttttgtattt ttagttgaga ctgggtttca ccatgttggc caagctggtc tcgaacccct 136260
gacctcaggt gatctgccca cctcggcctc ccaaagtgct gggattacag gcatgaaaac 136320
caccgcaccc agccttattt tatgtgtctt aattttctca tttatctctc tcactaacat 136380
gtaaatacca caagcataga aacctggttg ctcttttctc tggtgtatcc tcaacattga 136440
aagcaagccc tgggcccata atatttgttg aatgcataaa tcaagagttg tcttaactgt 136500
gtgtggaaaa tacgaattat agatcctgcc cataaagtaa ctcaaattta cctttaagga 136560
ctagcaaaga tgagtcattt aggaaaactc ctaacaaaca aatttataat taatcactta 136620
tctataaaac atttttaaaa attcatgttg cttttaggta ttttattacc atagtattgt 136680
acttaaaact aatatacatt ttaaaacttt ttattgggag gggcacggtg gctcacgcct 136740
gtaatcccag cacttgggga ggctgaggcg gatggctcac ctaaggtcag gagttcgaga 136800
ccagcctggc caatgtgatg aaaccctgac tctactaaaa atacaaaaaa ttagccgggt 136860
acagtggcag gcgtttgtag tcccagctac ccaggaggct gaggcaggag aattgcttga 136920
acccaggagg cagaggttgc agtgagccaa gatcatgcca ttgcactcca gcctgggcaa 136980
caagagcaaa actctgtctc attaaaaaaa agaaaaaaag aaaaaaagct gggcatggtg 137040
gctcacgcct gtaatcccag cactttggga ggccgaggta ggtggatcac ctgaggtcga 137100
gagttggaga ccagcctgac caacatggag aagccccgtc tctattaaca caaaattagc 137160
caggtgtggt ggtgcatgcc tgtaatccca gctactcagg aggctgaggc aggagaattg 137220
cttaaacctg ggaggtggag agtgcagtga gctgagattg cgccattgca ctccagcctg 137280
agcaacaaga gcgaaactct gtctcaaaaa gaaaaaaaaa aagaaaaaag agaaaacttt 137340
gtattatgga acaagtagtc aaacataaac aaaataaaag aaaatagtat aactattcca 137400
ggcaaaatat gattttagaa ttctatagcc caataatgtc aacatagatt ttgtgattta 137460
ggataaaaac attttgaaaa atacgatttt agaattccac agccaaacaa tgtccataaa 137520
taaatacccc aggcatccat tcattattca gcttccataa ttaccagttc atggccaata 137580
ttgttctatc ttttcctcca actccctcaa cctccactaa attattctga accaaatacc 137640
gggcatcata ttattaatat ttcatctcag tatttcagta tatgtctcaa aggacttaaa 137700
gaaaatccac aggcactaac atacttaaaa cattaagata attatttaat ataatcaaat 137760
atgtagtcag tgttcccact ttcctgagtg ttcccacttt cctgattgcc tcaaatgttt 137820
ctttttcaca atgggtttgt tcaaatcagg atccaaacaa gaacaaaaca cttcatttaa 137880
ttaatatgtt tccttttttt attttgtttt gtatcttttg aagatgatct tttttgaaga 137940
gtttattgaa gtattgcaaa cagaaaagta aacaagtgat aagtgtacag ctcagtgaat 138000
tttcaaaaac tgaacacatc cctgtaaaca cacccagaag ctctgcctta ttctccgttt 138060
cagtcactgt caccccaagg gtaaacacta acctgacttc taacacaata aattagtttt 138120
gcctggatta cagtttaata taaatgcact catacagtat atactctttt gggtctggct 138180
ttgtttaact atctggtgag atttatccat attttttaga ataatttttg tttgttcact 138240
ttcttttctt ttctttcttt tctttttttt tttttttttt tttttgagac agtctcactc 138300
tgttgcccag gctggagtgc agtggcgtga tttcaactca ctgcaacctc tgcctttcag 138360
gtttaagtga ttctcctgcc tcagcctccc aagtagcttg gattacaggg gcccaccacc 138420
atgccctgct aatttttttt ttttttttta gtagagaccg ggtttcacaa tgttggtcag 138480
gctggtcttg aactcctgac ctcagatgat cctcctgcct cagcctccca aagttctggg 138540
attacagaca tgagccaccg cgccggcctg tttgtttact tttttttttg agatgaagtc 138600
tcactctgtc agcaggctgg agtgcagtgg tgcaatcttg gctcactgca acctccgatt 138660
ccctggttta agcaattctg tctcaacctc ccaagtagct gggattacag gcatgcgcca 138720
caatgcccag ctaatttttt gtatttttag tagagacggg gttttcactg tgttagccag 138780
gatggtctca atctcctgac ctcaggtgat ccacctgcct tggcctccca aagtgctggg 138840
attataggcg tgagccaccg tgcctggccc atagcttgtc aattctaaat cttttattta 138900
tttatttatt tatttattta tttattttgt ttgtttgttt gagacggagt cctgctctgt 138960
cacccaggct agagtgcagt ggcagaatgt cggctcactg caacctctgc ctcccgtgtt 139020
caagagattc tcctgcctca gcctcctgag tagctgggat tacaagtgca tgccactatg 139080
cctggctaat ttttgtattt ttagtagaga tggggtttca ccatattggt caggctggtc 139140
tcaatctcct gaccacacaa tccatccggc tcggcctccc aaagtgctgg gattacaggc 139200
atgacccacc acgcctggcc aatttttgta tttttttagt agagatgggg tttcgccatg 139260
ttggtcaagc tggtcttaaa ctcctgacag gtgatccatc tgccttggcc tgccaaagtg 139320
ctaggattac aggcgtgagc cactgcacct ggcctctccc tcctcttttt tattttgcaa 139380
tttatttgtc aaaggagttg agtcatttat cctatagtgt tcccaacatt ctaaattttg 139440
ctgactgtat tctttttatt tttatttttt tgagatggag tttcgctctt gctgcccagg 139500
agcaacaaga ggagtgcaat ggagtgacct cggctcacta taacctccgc ctcctgggtt 139560
caagtgattc tcctgcctca gcctcctgag tagatgggat tacaggcgtg caccaccaag 139620
ctcggctaat ttttttgtat tttttagtag agacggggtt tcaccacgtt gcccaggctg 139680
gtcttgaact cctgacctca agcgatccac ccgcctcagt ctcccaaagt gctgggattc 139740
caggcgtgag ccagcgtgcc cagcctgctg actgtattct tacaatgtga cttaacatgt 139800
ttgtttctta tatttcctgt aaatggtagt tacatccacc gatttgatca gattcagcaa 139860
atatgcattt gagaagtgtt tactaagcac tgtgcttgct gctaggtggg atacaaaaat 139920
gactaagaca tcactcctaa tctattgttt attatgaata tgaaaggtta aggccaggcg 139980
tggtggctca cacctgtaat cccagcactt tgggaggctg aggcagctgg atcacttgag 140040
gccaggagtt ggagaccaga ctggccaaca atgcaaaatc ccgtctctac taaaaataca 140100
aaaagtagcg gctgggcgcg gtggctcaca cctgtaatcc cagcactttg ggaagctgag 140160
acaggcggat cacctgagat caggagttcg agaccagcct gaccaacatg gagaaacccc 140220
cgtctctact aaaaatacaa aattagccgg gtgtggtggt gcaggcctgt aatcctagct 140280
actcggaagg ctgaggcagg agaatcactt aaacctggga ggtggaggtt gcagtgagct 140340
gagattgcac ccttgcactc cagcctgggc aacaagagca aaactctgtc tcaaaaaaaa 140400
aaaaaaaaaa aactaaaaat acaaaaagca gccgggtttg gtggtgcaca cctgtagtcc 140460
tagctacttg ggacactgag gcagaagact cccttgaatc cgggaggcaa aggctgcagt 140520
gagccgagat catgccacta caccccagcc tgggtgacag agtgagaccc tgtctgcctg 140580
tttttttgtt tgtttgtttt gtttttgttt tttctgagac tgagttttgc tcttgttgcc 140640
caggctggag tgcaatgcca tgatctcggc tcacggcaac ttctgcctcc cagattcaag 140700
cagttctccc gcctcagcct tccaagtaac tgagattaca ggcatgtgcc accatgccca 140760
gttaattttg tatttttagt agagacagag tttctccatg ttggtcaggc tggtcttgaa 140820
ctcccgacct caggcgatct gccagcctcg gcctcccaaa gtgctgggat tacaactgtg 140880
agccaccatg cctggcaggg caggactttt taaattcatt tttgtgacta tcacagggta 140940
tgcacctaag ctcagtaaac atttgttgag taattaaaac cagctaaagg tgtttttgtt 141000
ttttgttttt tttgagacgg agtcttgctc tgtcacccag gctggagtgt agtggtgcaa 141060
tctcagctca ctacaagctc cgcctcccgg gttcatgcca ttctcctgcc tcagcctccc 141120
aagtagctgg gactacaggc gcccgccacc acacccagct aatttttttt gtacttttag 141180
tagagatggg gtttcaccgt gttagccagg atggtctcga tctcctgacc tcgtgatcca 141240
cctgcctcgg cctcccaaag tgctgggatt acaggcccga gccaccgctc ctggccccca 141300
tttttttttt caagataaga tcttgctctg tcacccaggc tggagtgcac tggtatgata 141360
atgactcact gcagcctcac ctcccaggct caagtgatcc tcccagctca gcctcccgag 141420
tagttgggac cacaggtgag tgccaccatg gctgagacta ttttcatttt ttgtagagat 141480
gaggtctgga gagtcttgga agaattctta cgttaaataa aaattcagga tgcaaaattc 141540
tatatccagg ttgagcatag tggcttaccc ctgtaatccc agcacttaag gaggtagagg 141600
cagaatgata gtttgagtcc acgatagttt gagtccagga gttcaagacc tgcctgggca 141660
acatagtgag actctgttct ccataaaaag ggggggaaag accaaaaaaa ccctatatat 141720
tcaattatga tctcaattac tgaaaatata caaatagaca actggaaaga aatacatcaa 141780
aatattaatg gtagtaattg ctgggtggta gaaatctgct atctctatac aggagttcca 141840
gaaagaaaca acagacgtgg caagtgaaat agtcaatgaa taattcaaaa acatttccca 141900
gactgaaggt aacaacttgc cagactgtgg ctcagcccaa ttaatgaaag tagatgcaca 141960
cattggctgg gcatggtggc tcacacctgt aatcccagca ctttgggagg ccaaggcggg 142020
cggaccatga ggtcaggaga tcgagaccat cctggttaac atggtgaaac cccgtcttta 142080
ctaaaaacac aaaaaattag ccgggcgtgg tggcgggcgc ctgtagtctc agctactccg 142140
gaggctgagg caggagaata gcgtgaaccc aggaggcgga gctttcagtg agccaagatt 142200
gcgccactgc acccagcctg ggcgacagtg tgagactccg tgtcaaaaaa aaaaaaaaga 142260
aagaaagaaa gtagatgcac accaaggcac atcattgcaa gatttctttt ttttgttttt 142320
tgtttttttt tgagacagca tctcactctg ttgccccagc tagagtacag tggcacgatc 142380
tcagctcact gcaacctcca cctcccgggt tcaagcgatt ctcctgcctc agcctcccaa 142440
gtagctggga ctacaggcac gcgccaccat gcccggctaa tttttgtatt tatagtagag 142500
atggggtttc accatattgg ccaggctggt ctcgaactcc tgacctcgtg atccacccac 142560
ctcagcctcc caaagtgctg ggattacagg cgtgagccac catgcccagc tgcaagattt 142620
ctttcttttt ttcttttctt tttttttttt tttttttttt tttttttgag acggagtctt 142680
gctctgtcac ccaggctgga gtgcagtggt gcaatctcgg ctcactgtaa cctccacctc 142740
ccaggttcaa gcgattgtca tgcctcagcc tccaagtagc ttggattaca gacgtgcatc 142800
accacgccca gctaattttt atatttttag tagagacacg gtttcaccat gttggccagg 142860
ctggtctcaa actcccaact tcaggtgatc cacctgcctc gggctcccaa agtgctggga 142920
ttacagacat gagccgccac accccgcctc attgcaaaat ttctagtcac tagaatgaaa 142980
tgctcagcta tgaacagaca taatgacctg aatacagata attgatttaa ccagaaattg 143040
taatattggg tattagagaa ggaaatgcag agaggatggt aaagtttgag aactaaacct 143100
cacctaccat aagatgtcaa taaataatgt ctaaaatgga ttaatcaaaa gataacggta 143160
taggtctggc acaggggtgg ctcatgccta taatcctagc actttggaag gctgaggcgg 143220
aggattgctt gagtccagca gcttgagacc agcctgggta acaaagcaat accctgtgtc 143280
tatgaacaac tttaaaaatt actgggtgtg gtggtgtgtg cctgtagtcc caggtactca 143340
tgaggctgag gcagtaagat cacttcaact gatgcagttg aggctattca ggaggtggaa 143400
gtggactgct ggaacccagg aggtcaaggg ccaggcgcgg tggctcatgc ctgtaattcc 143460
agcactttgg gaggccaagg tgggggaatc acttgagccc aggagttcaa gaccagcctg 143520
gacaacaaag tgagaccctg tctctacaaa aaatagaaaa aaatttgcca ggtgtagtgg 143580
catgtgcctg tggccccagc tactcaggag gctgaggcaa taggattgct tcaatgggag 143640
cagttgaggc tgcagtgata cctgatagag ccactgcact ctagcctagg caacagagtg 143700
agaccctgtc tcaaaaaaat aataaataaa taaataaata caaataacag tatatacaaa 143760
ttccttatga aaatagaaat gtgtaaatgc cagcaaaaac aactaaagtg gttggttgtt 143820
tctggggtaa aggtctagag gcattggtgt ggaataaggc tgagaaaata ctttttttgt 143880
tgttgttgtt acaagccttt tagaactatt taactttata aaacatttcc acgtttttct 143940
tttcttttcc tttctttcct ttctttcttt ctttcttttt tttttttttg agacaaagtc 144000
atactctgtc acccaggctg gagtgcagtg gcatgctctt ggctcactgc aacttctgcc 144060
tcccaggttc aggcagttct cctgcctggt attacaggta tgagctgcca tgcccagcct 144120
ccatgttttt ctttaagaaa aataaaaatc aatttaaaat agtttattcg agaagtacat 144180
atactcctga aagagaaatg ccagagcaaa cacgtgggca acgatgttgc attgttagaa 144240
ttcagtttaa ctagacttaa attattatta ttattattga gacggagtct tgctgtgtcg 144300
ccaggctggt gtgtagtggc gtgatctcgg ctcactgcag cctctgcctc ccaagttcaa 144360
gtgattctcc tgcctcagtc tcccgagtag ctgggaccac tggcacacgc caccatgcta 144420
aatttttttt tttttttttt tgtattttta gtagagacaa ggtttcacca tattggctag 144480
gatggtctca aactcctgac ctggtgatct gcccatcttg gccttccaaa gtgctgggat 144540
tacaggtgtg agccaccaca cccggcccca gctaatttgt ttgttttgag atggaacctc 144600
gctctgttgc ccaggctgga gtgcaatggt gtgatcttgg ctcactgcaa cctccacctc 144660
cagggttcaa gcgattctcc tgcctcagcc tcccaagtag ctgggattac agtcacgtgc 144720
catcacaccc agctaatttt tgtattttta gtagagatgg ggtttcgcca tgttggccag 144780
gatggtctcg aacccctgac ctcaggtgat ctacctgcct tggcctccca aagtgctggg 144840
attacgggcg taagcctccg tgcccagccg agaaatgttg attattaggc tgagtagttt 144900
gaactcaaca aaggcaatgt aaaatgacat ttcagggact gcttagtgca acaaaacact 144960
tacatggggc cattagaccc taactatctc tattagactt agccctgagg gcaagtacaa 145020
taattcagcc tgtatattca ctgccaggcc cacagtaagc atttcaagaa gagtatgaga 145080
aaagtggatg tgaccaggca tggtggctca tgcctgtaat ctcagcactt tgggaggctg 145140
aggtgggtgg atcacgaggt caggagttcg agaccatcct ggccaacatg gcgaaacctc 145200
gtctctacta aaaatacaaa aattagctgg gtgtggtagc gcgtgcctgt aaacccagct 145260
attcagaggc tgaggcatga gaattgcttg aacccgggag gcagaggttg cagtgagcca 145320
agattgtgcc actgtactcc agcctggcga cacagcagta ctccatctca attaaaaaaa 145380
aaaaaaagaa tacatgtgca cttagcaaat gaaaaaaact caatgggaaa gacagtagtt 145440
aagaacctgg gagttggaat gggaaagaat tctaagttcc ttccctaata gttaacctta 145500
tactatgatt aaagtgtaag tacagtgata tgctgtatga ctacagacaa acttatttgg 145560
ctagtttact gataaaatag tagaaaataa tagttcctac ttcacaaagt tgttctgaag 145620
attaaatgag atgatacaca caaagccttt agcatggagt ctgactcaca gtaaacccta 145680
ggtaaatggt agctgtatct attatcaaat ggagactctt gaatttcttg cgtcaaccca 145740
tgtgactgtt aacatatata ttgtttgaat atttgctaaa gagaaaggaa agacggccca 145800
gcagggtagc ttatgcctat aattccagca gtttaggagg ctgaggtggg aggattactt 145860
gagaccagga gttcaagacc agcctgggca gcatagggag acccccatat ctaccaaagg 145920
aaaaaaaaat tagccaggtc tggtggcttg tgcctatagt cccagctact caggaggctg 145980
agatgggagg atcccttgag cccaggaggt ccaggcttca gtgagccgtg atggtgtatt 146040
gtactccagc cttggtgata gagcaagacc ctgactcaaa aaaaaaaaaa gaaagaaaag 146100
gggatttttt ttttttttga gacagagtct agctctgtca cccaggctgg agtccagtgg 146160
tgccatctcg gctcactgca acctctacct cctgggttga agcgattctc ctgcttcagc 146220
ctcctgaata gctgggacta taggcgccct ccaccacacc tggctaattt tttttgtatt 146280
tttagtagag acggggtttt accatattta ttggccaaac tggtcttgaa ttcctgacct 146340
tatgatctgc ccatctcggc ctcccaaagt gctgggatta aaggcatgag ccaccgtgcc 146400
cagccagtgt actttatatg tatcccaggt actttatatg ttattttaat cctcacaata 146460
atagtaatta ttattatctc cattttacag ataaggaaac caaggctgat agaggtgaag 146520
taatttcctt caggtactca atatatattt attgagtaaa gccttacttt ggtgtaaggc 146580
ttgttcaaga cactgggaaa ataatactga acaaaaagac agggtcccta atgaaactta 146640
cattctatcc agggagacac acaatatatt gacaatataa tatattgtca atacacacaa 146700
tatattgcct gtagtgttat gaagaaaata gacagggttc tgagatagaa ggttataaga 146760
ggaaggccag gtgcagtggc tcacatctgt aattccagca ctttgggagg ccaaggaggg 146820
cagattactt gaggccagga gttcgagact agcctggcca acatggtgaa accgagtctc 146880
tattaaaaat ataaaaaaaa attagccggg cgtggtgggg cacttgtaat cccacctatt 146940
caggaggctg aggcacaaga attgcttgaa cccaggaggc ggaggttgcg gtgagccgag 147000
atcccgccac tgcacttcag catgggggac caggacaaga ctccgtctca aaaaaaaaaa 147060
aagaaaatta tgaagaatgt gctttagata gggtggtcag aagaggtgtt tttaaaagaa 147120
aaagttcaac tgagagctga agaatgagat gggtaagcca tgcaaagagc caaacagagt 147180
tcagggcaga gggtacgtca ggtacaaggg cctggccaca ggaaaaagct aggagctctg 147240
aagcatggta atgagatttt tggaaagtgc ctttagttgg tggtatggac aagggccaga 147300
tcatgaggac cctacagagc atggtaatga gtttggattt aattccaaat acaaatgaga 147360
aggtaatgta agattaaaaa gcagatgaag agcataatct tattttctta tttcttaaaa 147420
gacacttccc ttgctgagta aatgtagtgg aggagttggg ggtaggagaa aagggcagaa 147480
gagaaacttg ttaaggaggt agatgacatt tgttcaggga agagataaga gccttgtact 147540
agggtgatca tcggagggga gataaaatca acaggactcc actacacgtg gaggaatcaa 147600
aggttctggt tagcaccagt atccagatga gaaaagatta gggtggagat aggtcccatt 147660
ttgaaatgcc agcgagccac ctaagaggag atatcaatag acagctggct actcaaaata 147720
gagatgacca gcagaaggag ctgtagatgc agttatttcc ttggggcggg ttgtgcgggg 147780
gggcgcatgt aggggattag caagtggagg aacacaaaaa ggacatcaga gctttccact 147840
acaccgggtt gttacttaat agactaacac gacatttagg ttcccatggg ttgtgtgggc 147900
cagaagcgca gttccaggaa ccgactacaa catagtgtgg gatatgatgt ttctcttcaa 147960
ataacccgat caatctttta ttctttaatt catagtaccc tccctttttt ctctttttct 148020
ccttttttgc ttttgttaaa tgcccaggca cgccacaata ctaggcctta tcaataccag 148080
ctcacattcc tttccttatt tgaagggact aactttttag ctcattacag acaccccttc 148140
ccctttcctg tccgctttct tttaagtacc caccttagct aaaaaaaatc aaatgtttag 148200
ccaaccggga ttagtttaga ttatacgacc cgaccccagc caatgggaaa aagatacagg 148260
ggcaggactt gcgtcaaaaa tagactcctg cccctttgtt caggtgtgct ctcaaggcga 148320
ctggccaaaa aggcacccct ctgcgcaaaa ataaaattgc tttgctaaaa atcctttgtt 148380
caagtgttca atttccttaa gattttaaaa gttattcata acaataggct cctgtttcag 148440
tatttcctgg tgcggcgtac cacacctaca gcggtcgagt cacgccaggc actgccaaga 148500
cgccgaaaag ttctcccgca gcaggccgga ggtgaccgac ggcgcgcagg ccccagcgag 148560
cgctgcatgc tgggatccgt aggccggggc ggcggatgtt tgcgcagctt ccttgagatg 148620
ccaccgcccg ctgcgccgcg cagaaacggg gagcgcagcg cacccgcggc ctgcgtcact 148680
cggtcagtgc agtcgttcta aaatcgaggc ggaccctgct ttcttgcctg gagtcactta 148740
gagatgatga ctaaaggata aattcagagt cctccaaatg gcgtccagtg tccatttcat 148800
cacgatttgg aacctgtagt agccttatcg cttaacactt cctaattatt attattatta 148860
ttttcttttt gagaaagggc attgctctgt cgcctatgct ggagtaatcc agcgacataa 148920
tcccggctca ctgcagcctc tatctcccgt gttcagcgat ccttcttcct cagcatcccg 148980
agtagatggg accaaaaggc acgcatcacc acaccaggct attttttccc ctctattttt 149040
tgttgagacc cgttgggagg cgcggggagg ggggtctccc tatgtcgccc agactggtct 149100
caaactcctg gaacttaagt tatcgtccta cctcggcccg ttaaagtgtt gggattacag 149160
gcctgagcca ccgggcccca acattaatta ttcttcaagc ctattctgga ccgggcgcgg 149220
tggctcacgc ctgtaatccc agcactttgg gaggccgagg cgggtggatc atctgaggtc 149280
aggagttcga gaccagcctg gccaacatgg tgaaaccaca tctctactga aaatacaaat 149340
attagccggg cgtggtggca cgcacctgta atcccagcta ctcgggggct gaagcagcag 149400
aatcgcttga acctgcgagg cggaggttgc aatgagccga gatcgcgcca ctgcactcca 149460
gcctgggtga caagagcaag actccgtctc tcacacacac acacacacac acacacacac 149520
aaagactatt ctggtctctg cctccgggat ggcggctcgc tttggacctc tcttctgtgc 149580
ttctactgcc ctctcttaca attctctaat aattataatc atggcgagtg cttaccatat 149640
attccaatct atttacttcc acaagctcat taattctcac cctaaccctc tgagatggtt 149700
actattattt cctctctgca gctgagggaa ttttgggcta gggacgttat gtaagttgag 149760
ccacgctacg ctaaaagttc cacactcaat tctagcgtct cggctctgga ctaccaagtt 149820
ccggagcaag cagacagacc acctctttac gttcccgtag gccacgctcc gggggcgggg 149880
ctccagtgag gatactgcat cccatggtgc cttgcgcgcc agcgcagcca ttggtccggc 149940
tactctgtct ctttttcaaa ttgaggcgcc gagtcgttgc ttagtttctg gggattcggg 150000
cggagacgag attagtgatt tggcggctcc gactggcgcg ggacaaacgc cacggccaga 150060
gtaccgggta gagagcgggg acgccgacct gcgtgcgtcg gtcctccagg ccacgccagc 150120
gcccgagagg gaccagggag actccggccc ctgtcggccg ccaagcccct ccgcccctca 150180
cagcgcccag gtccgcggcc gggccttgat tttttggcgg ggaccgtcat ggcgtcgcag 150240
ccaaattcgt ctgcgaagaa gaaagaggag aaggggaaga acatccaggt ggtggtgaga 150300
tgcaggtagg gagagggctg acaggattcc gagcgctgcg gcttcgctgc tgggccccct 150360
actgcgcggt ccagggagag ggattttatt tgcatttcct gagggtccca gtttcttggt 150420
tctccgcgtt ctgttcaata aaaatgacac ccggttgctg tgtgtatgtg gtttttagga 150480
gaaaataaca tgtttgattt gatcactgtt ccatactgaa aagtgcgttc ttatgtttaa 150540
actatagtca ataaagatgt aggtgtcact tttatatgct acttcatgta gtttgtcagt 150600
ttggaagtaa gactgaatac ctattttgca gatggttaag cacttacgtt ggtattatta 150660
cctctaaaaa gcaatcaatc actgttctct ttattctaga tgcaatattt tctgcatccc 150720
tactaaacaa gtaaacatct ctgtctttaa acattaaaaa ttaataaagt gagtttatgt 150780
tttttaaaag atcaagacaa gttaagctag tgtaaatttc tgattgtgct gcagttttcc 150840
ctttcatctg cgtgtttgtt catagaccaa actaaacgga tatttcgaga taaagggatg 150900
tggaggggtg ttcggccctc ccctgactgg tttccaaata acttcccaat tttaaaataa 150960
gctagaaact gctgcttagt tagaattgat ttattcaact ttttagaggc tctccacagt 151020
ttactgagta tgtgtgtttt gtgtgtccgt ttttgtggct gttaacagtt gctgttgcaa 151080
cttttctgta aaccccaaag tattctaaaa ttaaaaggtt attttttaga aaaacttgta 151140
tcaacaaact ttcttaggct attgtttact cagccctggt aacttgaatt gtggatatcg 151200
aaggtgatga ctaggtttct aaacaaggtg tcagagagga aattgggtgg caatcttaag 151260
tctagccagt gacacttact agttgttgta ctctggggct tctgttagta gttagttgtt 151320
tgaagatttt tatttaaaaa tgcagtgcat ataacaagtg caaattttaa aattattttg 151380
gttgtttaat aaaaccaggc aatattacct tttccgtatg catttattta atttcttttt 151440
taatgttagt tacaaaatat ttcagactta caaaaagtta ttaagaacaa tgtaataaac 151500
atttgtatat tcatcaccca gattaagaaa caaaacaatc attgctggag cccccctttt 151560
ccctctccat cccttttcct ccagccactg ttaaccatta tcttgaactt gatgtttatg 151620
atccgtatac atttcaagtc ttagaagaga tttactttat caccaatgaa tagaatagtt 151680
ctttgcttgt gtctaatatt tacaataatt tttgtgtaaa gagatttttt ttttcagatt 151740
agcctttttc tttcaaatag ccttcctggt gcgtctgtca ttaagcatta agctttagta 151800
aataggcaac ttatgagttg cgaggtcctc aggggttggt aagccttttc tgtaaagggc 151860
ccagagaaat aaatatttat tttatttaaa tactttaggc tttgcagatc acatgtggtt 151920
tatcagtttt ttttgagacg gagtctcgct ctcttaggcc gtagtgcagt ggtgcaatct 151980
cagctcactg caacctccgc ctcccggatt caagcaattt tcccacttca gcctctcagg 152040
tagctgggat tacaggcgca caccgccatg cctggctaat ttttgtattt ttagtagaga 152100
tggggtttca ccatgttggc caggctggtc tcgaactcct gacctcagag aatccaccca 152160
ccttggcctc ccaaagtgct gggattatag gcgtgagcta ctgtacccct ttacatgtgg 152220
tttctgtcac attattttct gatctttttt tttttaagaa cactttaaaa ctgtaaaaat 152280
cattcttaaa tctgtcaggg caggggccag gtttgaccca caggttaaag tttacagatc 152340
cctacataat gcgctactgg ttctctttct tatgtccctc ctattccatc ccctattttt 152400
ttcccaattt aaaaagtcat tttcaaacac acataatata aaatttaaca tcttaaccac 152460
ttttaagtgt acagttcagt gatattaaat acataatatt ctgcaacaat tactaccatc 152520
catctccata actcttttca tcatgaaaaa ctgaaactct atacacatta aacaataact 152580
ccccatttcc ctctcccatc aacccgacac ctaccattcg actgtcttat gattttgact 152640
actctaagta cttcataaag tggagtcata atacagtatt tatccttttg tgacttgctc 152700
attccactta ggcataatgt cctccaggtt ggaccatgtt gtagcaaatg tcagggtttt 152760
cttccttttt aaggctgcat agtatttatt atatgtatat accacatttt gcttgtccct 152820
tcatatgtca atggatactt gagttacttc tttttttgtt gttgttgttt ttgagaccga 152880
gtctcgctct gttgcccagg ctggagtgca gtggcgcggt cttggctcac actgcaagct 152940
ccatctcccg ggttcacgcc attctcctgc ctcagcctcc caaggagctg ggactacagg 153000
cgcctgccat ctctcccggc taattttttg tatttttagt agagacgggg tttcaccata 153060
ttagccagga tggtctccat ctcctgacct tgtgatctgc ccgccttggc ctccctaaga 153120
gccgggatta caggcgtgag ctacggagcc cggccttgag ttacttctta cttttagcta 153180
ttatgaataa tgttgctatg aatatgggtt ttcaattctt ttgggtattt acccagaatt 153240
gtaattgctg gatcatatga taattctatt ttaaagtttt tgaggaaccg acaaactatt 153300
ttccacagtg gctggaccat tttacattcc taccaacagt gcataagggt tccaatttct 153360
ccacatcctc accaacgttt attttctgtt tctttttttt ttttcaagta gccatccatt 153420
gggtgtgagg tgctatctca ttgtagtttt gatttgtatt tccctaatga ttaatgatgt 153480
tgagcatctt ttcatgtgtt tactggccat tttgtgtatc tttggagaaa tgtctgttta 153540
agtcctttgc ccatttttaa attggtttgc ttttttgttg ttgagtttta ggaattttct 153600
atatattttg gatattttca gatacataaa cggcaaaaat tttttcccca ttactgtggt 153660
ttgccttttt actcattgat accgtgtggt cttttccttt ctttttcttt ttggaaccag 153720
tgcatggcct ctttgttgat tctgtgtttg gccccagtgc agcctgttct gtgctatgtg 153780
tctgcagtgc tgaaaccagg cctacccagc accatacaga agtccaggct gtagatacca 153840
atgcatgggt cacatttgat acccaaatct gtgtgttcct ggatctccaa accaaagttt 153900
ccagtatctg agaagttgtt ctttcttgat tcacactcct gcatctttag acctttcttc 153960
agggtttctt ctgctttggc cctttgtgca gtgggtggca atcttcactt ctcctgatgc 154020
caaaggatct gacaatgtat ttgactttgg agaacacagg ggtctggcct atgagctgct 154080
ccaacacctt ggctgctggg gtcagtcatc tccagtctcc tccatacaga tgttgagaca 154140
gaaaatcgtt ctgtcaccca ggctggagtg cagtggcaca gtctcggctc actgtaacct 154200
ctgcctgtca ggttcaagca attcttatgc ttcagcctcc caagtatctg ggattacagg 154260
tgtgcaccac catgcctggc taattttttg tatttttagt agagatgggg tttcgccttg 154320
ttggccaggc tggtcttgaa ctactggcct caagtgatcc acccattttg gcctcccaaa 154380
gtgctgggat tatgagtgtg agccctcatc tggccagagt tctctttttt ttaccttgat 154440
cttgcactat gatggagaaa aggaagataa agtctttttt tttcccttgg gtttgtttgt 154500
ttgtttgttt gtttgttttt tgagacggaa ttttgctctc gttgcctagg ctggagtgca 154560
ttggtacgat cttggcttac tgcaacctcc gcctcctggg ttcaagccat tttcctgcct 154620
cagcctctag agtagctggg attacaggca tgcgccacca cgcctggcta attttgtatt 154680
tttagtagag atggggtttc accatgttgg tcaggctggt cttgatctcc tgatctcagg 154740
tgatccgtcc accctggcct cccaaagtac tgggattaca ggcgtgagcc cgtgcctggg 154800
ctattttttt tttctcccct ttaaatatag tatcttgctt tattgcccag acttgttgtg 154860
aactcctgga ctcaaatagt cctcctacct cagccttcca ggtagctggg atcacaggga 154920
tgctgtcttt tgatacacaa acattttaaa tttttatgaa gtccagtttg tctttttgtt 154980
tttgttccct gtatctttgg tgttatatcc aagaaatcat tgccaaatcc attgttgtga 155040
agcttttgcc ttatgttttc ttctaagagt tttatagctt taggtcttac atacattttt 155100
gatccatttt gagttaatat ttgtatattg tgttagataa gggtccaacc tcattctttt 155160
gcatatggat atttagtttc ccagcaccat ttggtgaaaa gcttgtcttt ttctgattga 155220
atggtcttgg caaccttatt aaaaatcatt tgctcatatg taagagggct tatttctaag 155280
tgctgttatg ttccattggt ctataagtct gtctttatgt cagtaccaca tggttttgat 155340
tattgcagct ttgtagtaag ttttgaaatc aggaagtgtg agtcctccag ctttgttctt 155400
tttcaagatt gttttggcta tctggactcc cttgggattc catatgaatt tgaggatgaa 155460
tttttctatt tttgtaaaac acgtcattgg gattttaata gggattacat tgaatctata 155520
gatcactttg ggtagtattg gcatcttaac aatattaagt ctttcagttc atgaacaagg 155580
gatgtgtttc catttattta tgccccttaa tttctgccag cagttttttt ttgtttgttt 155640
ttgttttgag atggagtttc gctcttgttg tccaggctgg agtgcagtgg cacaatctta 155700
gctcattgca acctccacct cccgagttca agtgattctc ctgcctcagc ctcctgagta 155760
gctgggatta caggtatgtg gcaccacgac cagctaattt ttgtattttt actagagaca 155820
aagtttcacc atgttggtca ggctggtcac gaactcttga cctcaggtga tccacccacc 155880
ttggcctccc aaagtgctgg gattataggc gtgagccact gcgcctggcc tcagcagtgt 155940
tttatagttt tcattttata agtctttcac ctccttggtt aaattaatta ctaatatttt 156000
attctttttg gtgctatttt aaattgagtt gtttttgtaa tttccttttt agattgttca 156060
ttcttagtgt ataaaaatgt aactggaggc tggacgcagt ggctctcacc tgttatccca 156120
gcactttggg aggcaaggtg ggcctcccaa ctcctgatca cgagatcagg agttcaaggc 156180
cagcctggcc aacatagtga aaccctgtct ctactaaaaa tacaaaaatt agccatgcat 156240
cgtggcgcgt gcctgtagtc ccagctactt aggaggctga ggcaggagaa tcgcttgaac 156300
ctggaaggtg gaggttgtgg tgagccaaga tcgtgccact gtactccagc ctgggcaaca 156360
gagtgagact ctgtctcaaa aaaaaaaaaa aagaaaaaag aaacgtcgct ggattttgct 156420
tgttgacttt ctatccagct actttgctga attcacttat tagttctaac agtttttttt 156480
tgtgtgtgtg taattttaga gttttctttt cttttttttt ttttgagatg gagtctcgct 156540
ttgttgccag gctggagtgc agtggcgcga tctcggctca ctgcaacctc tacctcccag 156600
gttcaagcaa ttctcctgcc tcagcctccc gagtagctgg gactacaggc acacgctacg 156660
acgcccagct aatttttgta tttttagtag agacggggtt tcaccatgtt ggccaggatg 156720
gtctcgatct cttgacctcg tgatccacct gcctcagcct cccaaagttc tgggattaca 156780
gccgtgagcc actgtgcctg gctttttttt ttttttaatt aactattgaa cttctgttta 156840
ttattattat tattatttat ttatttatta ttttttgaga tgaaatctcg gtctgttgcc 156900
caggcgggag tgcagtggtg tgatctcggc tcactgcaac ctccgcctcc cgggttcaag 156960
caattctgtg cctcagcctc cggagtagct gggattatag gcgctcgcca ccatgcccgg 157020
ccaatttttg tatttttagt agagataggg tttcatcatg ttggtcaggc tggtcttgaa 157080
ctcctgacct cgtgatccgc ccgcttcggc ctcccaaagt gttgggatta caggcgtgag 157140
ccactgcact tggccttatt tttattattt ttttcatcaa cttttaagtt ctggggtaca 157200
tgtgcatgat gtgcaggttt atcacatagg taaacctgtg ccatcacagt ttgctgcaca 157260
gatcaaccca tcacctagct attaagccca gcatccacta gctattcttc ttgatgctct 157320
agctcctttt gccccactga attttagggt tttctttctt ttctattttt ttttcttttg 157380
agacagactc tcgctctgtc accaggctgg agtgcagtgg cacaatcttg gctcattgca 157440
acctctgcct cctgggttca agcgattctc ctgcctcagc ctcccgagta gctgggacta 157500
caggcatgcg cccatgccta gctaagtttt tgtagtttta gtagagacag ggtttcacta 157560
tgttggcaag gctggtctca aactcctgac ctcaagtccc cttggggttc ctgaagtact 157620
gggattatag gtgtgagcca ccacgcccgg ccagattttc tatataaaag atcatataat 157680
ctgcagacag ataattttac tttttccttt tctttctttt tttctttttt tgagatggag 157740
tctcgctctg tcgcccaggc tggagcgcgg tggcgccatc tctgcttact gttacctctg 157800
cctcctgggt tcaagcagtt ctctgcctca gcctcccaag tagctgggat tacatgcaca 157860
tgccaccacg cccagctaat ttttgtattt ttagtagaga tgggagttca tcatcttggc 157920
taggctggtc ttgaactcct gacctcgtga tccatccgcc ttggcctccc aaagtgctgg 157980
gattacaggc atgagccacc gctcttggcc ctcaaccttt tccttttcaa tttggatgcc 158040
ttttattttt atttttcttc cttttttttt tgagatggag tctcgctctg ttgcccaggc 158100
tggaatgcag tggtgcaatc tctgctcact gcaacctacg cctcctgggt tcaaacgatt 158160
ctcctgcctc agccttccga gtagcttgta ctacaggcat gtgccaccat aaacagctaa 158220
tttttttttt ttctcgtatt tttagtagag acggggtttc gccgttttag ccaggctggt 158280
ctcgatctcc tgacctcgcg atccgcctgc ctcagccttc caaagtgctg ggattacagg 158340
cgtgagccac catgcccggc ccttgttttt ctttcttgcc taattactct agctagaact 158400
tacagtatta tgtcgaatgg aagtggcaaa agtgggcatt gggcatcctt gtcttgctcc 158460
tgttctttta tttgttagtt tgtttgagat cctcctgcct cagtttcctg agtagctggg 158520
actatagaca cactactaca cccagctaat taaaaaaata attttttttt tttttttaga 158580
gatggggtct cgctatgttt cccatgctga tcctgaactc ctggcttcgg gtgatcctct 158640
actcttaccc tcccatagtg ctgtgattac aggcatgaac cactgtgctg gccctgttct 158700
tgtttttaga ggaacattct tcagtctttg accatcaatt atgtttgctg tgggtttttc 158760
atatgttgct tttattttgt tgaggtagtt tcactctatt cctagtttgt tgagcatttt 158820
tatgactaat gggttttgaa ttttgtcaca tgcttttcct gcatcgattg acatgattct 158880
gtggtttcct tcattctgtt aatgtggtat attacattga tcaattttta catgttggac 158940
catccctgta ttccaggaat aaatcccact tggtcatggt gtataatcct tgtgctgctc 159000
agttcaatgt gttggtattt tgttgaggat ttttttttat cagtgttcat aagggatagt 159060
gatctgtagt tttcttgtag ttgcctttgt ctggctttgg tatcagggta atgcttgcct 159120
cacaaaatga gttgggaagt gttctctcct ttgccagatt ttttctggga aaagattgag 159180
aagaactggt attagatctt cttgaaatgt tttatagaat tcacctatga aactatcaga 159240
tctagggctt ttctttgtca ggagattttt ggttagtgag tccatctctt tactggttat 159300
agctccattc agaatttcca tttctttgtg atttagtctt tgtaagtatt gtgtttctag 159360
gaatttgttc agctgggtta tccgatttgt tggcatacaa ttgttgaaaa tactctttca 159420
acaataagag agaagacaca aataactagt tcttttgttt ccagttcctt aagttgtaaa 159480
gttagggtgt tgatatgaga tctttcttgc tttttaatgt aagcattcat agctataaat 159540
ttccccctta gcactgcttt tgctgtgtcc cgtaagtttt ggtatgttgt attttcattt 159600
tcattaatct ctaaaatttt ctaattttcc ttgtgatgtc tttgaaccct ggttacttaa 159660
acacacacac acacacacgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgg ttttttgttt 159720
gttttgagac tgagtttcgc tcttgttgcc caggctgggg tgcaatggcg cgatctcggc 159780
tcactgcaac ctctgcctcc cggttcaagc gattctcctg tctcagcctc cagagtagct 159840
gggattacag gcgcctgcca ccacgcctgg ctaatttcac acacacattt aaaaaataca 159900
tctacctgct tttacttcag aatctttgca atttctgttc tctctgcctg aaaatttttt 159960
ccaccaaaat atctacaggg cctggctccc ttgcttttta ggttctgctt aaatatcacc 160020
tgcgtagaag cattccctaa ctaccctaaa atagcaacca actatcttcc accctcaaca 160080
cttcctatcc cccttaaact gctttctttt cttttctttt tttttttttt tttttgagac 160140
agagtctcgc tctgttgccc aggcctggag tgcagtggcg cattcttggc tcaccgcaac 160200
ctccacttca gcctcccaag tagctgggac tgtaggtggc tgccaccatg cctggctaat 160260
tttttttttt ttgagatgga gtctcactct ttcacccagg ctggagtgta gtggcacagt 160320
ctcggctcac tgcagcctcc atctcccagg ttcaagtgat ttctggctaa tttttgtatt 160380
tttagtagag atgaggtttc accatattgg ccagggctag tttcgaactt ctgacctcaa 160440
gtgatctgcc caccttggcc tcccaaagtg ctaggattac aggtgtgagc cactgtgcct 160500
ggcctaattt ttgtattttt aatagtgatg ggtttttact atgttggcca ggctgatctc 160560
aaactcctga cctcaagtga ttcacccacc tcagcttccc aaagtgctga gattacaggc 160620
gtgagccact gtgcctggcc tgctttattt tcatttctaa tttatcagca tctaatatat 160680
ttattcattt tatagtcatc tcttccttca actaagttaa aagctccctg agcacaggat 160740
tttggtgttt ttttctttcc tttaactttg tattgttcat tgctgtatct ccagtgtcta 160800
gaaaattacc tatcacatag taagtacttg atgaatattt gtggaatgag ttaatttagc 160860
acttctccac aggataggac ttaggttttt ttaatcctca atctcccttc cctcaccatt 160920
ttgattgttt gaatttttat ataactatgg tgaagccaac aaattgatgg atttgttagt 160980
gtgagcagcc gaaactttgc aatttctaat aagttagaga agtctgggta ggaaactaat 161040
gacttggcag tactctttct tagagtacac atagtcccta aagcttctct gagaattttg 161100
ataactttga ggaatgtgtg atctgtatga atttcctatc acttagtcct gacaatgtga 161160
atggtattca tttggtaact taattttata cgtccaggca agatactagt ttaggggatg 161220
ccaaaaataa tagactaatt ggaaaagctt tagccacatg agagcaattc actccacttg 161280
atgctcttgg cctacctcag tataagttgg ttctacctta gttttgttga agttttaata 161340
atactgtaca ttcatgttgg ttatatgcat tgtgtaagtt ttagtatagt tggcaaatga 161400
aagcattacc agatactacc tgggagttaa gtttcctagg atcacagatt tggtcttctg 161460
atcacttgga agtatactta gagtgggctg tgccagggga agttgaggta tccttcttaa 161520
ataagtagca acttggttta tctagtgata agggggaaat aatttcctgt ttggcacttt 161580
ctccaaaata tatgatactc aatgggaaaa atgaactcag gtcaagatta tgtctctccc 161640
tttggcccag acatgtattg agtatataat tgtcttattg atgctactct gtggactgtg 161700
atattagttt tcccataatt cctcttagga tgacatttat taggcaatgt agtttaacag 161760
atatttaaga acctactgtg tgctaagcat ggtagttgtt gctggggaaa cagtaaacta 161820
gacagtattt ctttctgtag tgatctgagg tttagtgggt aacacattaa aaaaaaaaga 161880
taagagaggt gatgtttaga aaaggtgtat aaagggtgct gtaggaatat atagcagaca 161940
tttaatgtgg tcttggttgg agcaggtggg ggaggcacat aggatagaaa ggacttcctg 162000
aggaaataat catttcaact aaatacctac tcaggcattt ccgtagaatg aggactcttg 162060
atccagtcgg cagtgtagac cttctgtgtc tattcactca tttaaaaatg gggctaataa 162120
tattaccaac ctcacagtgt tgtgtggaca aactgagtga gcacaatgca aagcacttga 162180
aacaataagt acctggcaca tagtaagtac tcaacttatt agtcattatt tttatgtact 162240
ttttattttg tgccaggtat ctactggcag attagtattt tgaacacaaa tttgacatgt 162300
ttttttctac tacatcataa cctaatttga tcggattttt tttttttttt ttttttgaga 162360
cggagtttcg ctcttgttgc ccaggctgga gtgcaatggc gcgatcttgg ctcaccgcaa 162420
cctccacctc ccaggttcaa gcaattctcc tgcctcagcc tcccgagtag cagggattac 162480
aggcatgcac caccatgccc agctactttt gtatttttaa tagagacaga gtttctgcat 162540
gttggtcagg ctggtctcga actcccgacc tcaggtgatc cgcctgcctc ggcctcccaa 162600
agtgctggga ttacaggcgt gagccactgc gcccagcctt gatcagattt taagaatagg 162660
agaaatggaa ttctgaaaaa taagtttggc atttttacaa gttaaaacct gttagtggct 162720
gggcacagtg tctcacttct gtaatactag caccttggga agctgaggtg ggataatcac 162780
ttgagcccgg aagtttgaga ccagcctgtg caacacagca agaccccatc tctaccatca 162840
aaacaaaaca aatctattcg tatctgctct aagaagctgc cagaattgta atgtcttaac 162900
atatctttga acatttaaaa aattatattt gtgaaacttg agacacttat aattttactg 162960
tctgctttag caatatcact tagtcctgat ttaatctcca tcaccaacaa tgtggtcaaa 163020
atttcgcatt tttcttgaca aatgtagtta gtgaaaatgt cattgataac ctgagaacta 163080
agagctcttg aatgactttg tgtatttctt tttatagacc atttaatttg gcagagcgga 163140
aagctagcgc ccattcaata gtagaatgtg atcctgtacg aaaagaagtt agtgtacgaa 163200
ctggaggatt ggctgacaag agctcaagga aaacatacac ttttgatatg gtaacatatg 163260
gtgcaatttc tttattatcc actaatgtaa aataatttta atatacatat tttacctgga 163320
aaatggtgta tacttagaaa tttcagttgt ctctgaattg tcagatggct tctagtgggc 163380
tgaattatga attagttaac atacgaaaaa caaaattatt aaaatgagta attttgaggt 163440
tgattttttt ttttttaatt tttttcgtta ggtgtttgga gcatctacta aacagattga 163500
tgtttaccga agtgttgttt gtccaattct ggatgaagtt attatgggct ataattgcac 163560
tatctttgcg taagtaaaag ggtgtttttt ctgatttatg aaaaagctta aatgcttgtg 163620
ttttttgttg ttgtttgttt gttttttgag acggagtttc actcttgttg cccaggctgg 163680
agtgcaatgg cgcgatctcg gctcactgca gcctctgcct cctgggttca agcaattctc 163740
ctgtttcagc ctcccaagta gctgggatta cagctgcctg ccaccatgcc cgctaatttt 163800
tgtattttta gtagagacgt ggtttcactg tgttggccag gctggtctcg aactccggac 163860
ctcaggtgat ccgcccacct cggcctccca aagtgttggg attataggct tgagccactg 163920
tgcctggctt gttttttgtt tttctagtct atcactaaga gtcatatggg tgcatgtttc 163980
tttttgattt aacacttgtt aatctttaca ggtatggcca aactggcact ggaaaaactt 164040
ttacaatgga aggtgaaagg tcacctaatg aagagtatac ctgggaagag gtatttattg 164100
tttataacat acttttatct ctaatgtgac tgaaatttaa ctgtataaaa cttgtttgag 164160
ggcctctgtc ttggaataga gatcagagta cctatgtcaa aatgaactta ggataaacca 164220
ctactacagt aaaattaaag tgcatggtat gactcctgtt taagaaacag cctcaatgga 164280
agaggaagga ccaatatata tggcacagtt atatgataaa agaggagtct atttatgaca 164340
gaatggttgg agcagaatat tgtagaaaag ttggaatatg agtgaagctt gaaggcaggg 164400
agggctttgt attgaaggaa tgggtctcag aaagttagca tggccagggg aagtatagta 164460
ctttattcat gatgatccta agtgttcaag aaattaagat gaatgtattg tttaatattg 164520
cagaaagcat atactatgtt ttacaaagat ttccatgaat ttaagtgagt ttaataacta 164580
agagagagaa ccaatactgg aagaaagttg aaagaagacc aagacaagtt gaactagagc 164640
tgaagtgtta aaacttttaa aaaaaagttt agttttaaat ttagtaatgt gtttaaattt 164700
aaatgagttt aataactagt taattggtcg ggcacggtgg ctcatgcctg taatcctagc 164760
actttgggag gctgaggcgg gcagatcacc tgaggtcagc agttcgagac cagcctggcc 164820
aacatggtga aacccggtct ctactaaaaa tacaaaaatt agctgggtgt ggtggtgcat 164880
acgtgtaatc ccagctacct gagaacgaga ctctgtctca aaaaaaaaca aaaaaacaaa 164940
aaaccaaaaa actagttaat ttaaaaaaaa aaagaatttt ttttaatttt tttttttttt 165000
tttaaatttt aaagtgatgg ggtcccactg tgttccccag gccagtcttg aactcttggg 165060
cttcaggagt cctcccactt cagcctcctg agtagctggg attagaggca catactacct 165120
tgcccagcta attttccaaa ttattgacag ttgggtagaa cctttcttct agtggttaca 165180
taattgagtc attaacttac tttacatata tagataataa agttatgaaa ttgttaccat 165240
aggagatatg gaataggctt aaagcatagt ttcgctgggc agaattattg aacttggcgt 165300
tttttttttg agacggagtc tcgctctgtc gcccaggctg gagtacagtg gctcgatctt 165360
ggctcactgc aacctccacc tcccgggttc aagtgattct cctgcctcag cctcctgagt 165420
agctgggact acaggtacat accaccaagt ccggctaatt tttgtatttt tagtagagat 165480
ggggtttcac cgtgttggcc gggatggtct tgatctcctg accttgtgat ccacctgcct 165540
tgacctccca aagtgctggg attataggcg tgagccaccg tgcctggccg aacttggctt 165600
tttaagtaga taggttccat tggtttctat gtaatgctca gggatgaaga tggctaagaa 165660
gtgtgacagg tttatggagg gtgtgaagac tactgtagaa cagactgttg taaatgactt 165720
ctctaacatt aggttagttg tttctttttt tgttttgttt taacttgctt tgccatactt 165780
atgtttaaat atattataaa ggaggcccat gtattttaac tgccacagta aatggcattc 165840
ttcctttata ttagtcctta ttataatttc aggatccctt ggctggtata attccacgta 165900
cccttcatca aatttttgag aaacttactg ataatggtac tgaattttca gtcaaagtgt 165960
ctctgttgga gatctataat gaagagcttt ttgatcttct taatccatca tctgatgttt 166020
ctgagagact acagatgttt gatgatcccc gtaacaaggt aattcagtct ttgagaatga 166080
aatgtctctg aattttaatg tgtgaggctt tgagaagtca gagagagaga gagagagaga 166140
gagagagaga gagagagtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtttta 166200
accaatctaa tggatgttct tttggtattt tggtcagaga ggagtgataa ttaaaggttt 166260
agaagaaatt acagtacaca acaaggatga agtctatcaa attttagaaa agggggcagc 166320
aaaaaggaca actgcagcta ctctgatgaa tgcatactct aggtaagaaa gccatagtct 166380
cttccctagc cccattttct tttaagaaga attaggaact tggagaaagt caaattgggg 166440
tgggtcaggg tatgtgggtc acgtacctag agtttgtgtt ataaggaggg gtcattggta 166500
attggcttga gatttatatg gaaggaacca atattggaag aatgttgaaa ggagaacaaa 166560
gataagttga accatagctg aagtattaaa cttttttttc ttttttgaga cgaattttcg 166620
ctcttgttac ccaggctgga gtgcagtggc gcgatcttga ctcactgcaa cctctgccca 166680
ccgggttgaa gcaattctcc tgcctcagcc tcttgagtag ctgggattac aggtgtctgc 166740
cactacgccc agctaatttt tgtatttttg gtagagatgt ggtttcacca tgttggccag 166800
gctggtctcg aactcctgac ctcaggtgaa ccacctgcct tggcctccca aagtgctgag 166860
attacacgtg tgagccacgg cgcccggcct aaactttttt aaagtagaag aatcctttta 166920
ttctagtaac atgatatatg gaagcccagg ataaaatgtg gctgctatag ttgattttgg 166980
aatgggacgc tctgaggctt acctccttag ccttatgaag gggtccctga ggcacttttg 167040
tggagccccc tactgatatt agaatatagt ttgaaaaccc ttgcattgaa taaggattag 167100
aagttaggtc ttttaaaatg gctttatttt agtgtgggag acagatggtc actaacactg 167160
gctttcatgg ggtgaaaggg atgatttttt tcgctctaaa tatcttttac cgaaatataa 167220
aattcctttt taaaaaattg tttattttcc tctcttacca ctctattcat tgagatataa 167280
aatccctgtc ataaatttac gtaagttaga aactcctttt ttcttgtaaa gacccgtaca 167340
ggacaaattc caaattctac agatatagtt tactaaaaca gaagtggttt ttttgtgtgt 167400
gtgtgtggtc tttaaacctc agaatgtaat agaaaaagca atggattgca attagatatt 167460
tgcgtgtagt ctcagtttcg cggttaattc agtatgtgac tttttgtaaa taagtgaatt 167520
tatctgctta tcagtttctc tgagctacaa aattattgtt aggattagaa gtcttatttc 167580
ttttctggct tgtagttgaa aatttctata aaatgccata acaattacag ttgcaactct 167640
aaaaaagttt gcatttaaag aaagaagaaa aatataacat aaaagtgatt aagatcatgg 167700
aattttggat gatttcaaaa ttttaattaa attttcactt aatggctttc caataaaatg 167760
gaaattttat tctgtggttg attataactt aatttcatgt agaattttga gaactgaact 167820
aaagactagg taaaatttct ttaggtacat ttcactaaag tataaaattt ctatttttcc 167880
ttttttcttg tatgtagact tgtataaagg tcacttttta tgaaggtatg tgacaaagag 167940
gagaagctaa ttaattcagt tttcccaaat tagagctaac ttcaatgctt atttgtatta 168000
attgcctaat ctggattagg aatgggtaga taatggtaga aaaacatgag atgaatagta 168060
ttattattat tattattatt tttgagacag agcctctctc tgtcccccag gctggagtga 168120
agtgatgcga tctcggctca ctgcaagctc tgcctcccgg gttcacgcca ttctcctgcc 168180
tcagcctcct gagtagctgg gactacaggt gcccaccacc acgcctggct aattttttgt 168240
atttttagta gagacggggt ttcactgtgt tagccaggtt ggtctcgatc tcctgacctc 168300
gtgatccgtc cacctcggct tcccaaagtg attacaggcg tgaggcacca tgcccggtgg 168360
agatgaatat tataattcag atctatagtt tacatttatg tttttcctta ggtcttcctc 168420
cttttctgta attttaaata atttaaataa ttttataaaa atgatacttg gctgggcgcg 168480
atggctgacg cttgtaatcc cagcactttc ggaggccaag gtgggtggat cacttgaggt 168540
ccggagttca agaccagcct ggccaacgtg gtgaaacccc gtctctacta aaaatacaaa 168600
aattagcttg gtgtggtggc gtgtgcctgt aatcccagat actcggtagg ctgaggcagg 168660
agaactgctt gaacccagga ggcagaagtt tcagtgagct gagatcatgc cattgcactc 168720
tagcctgggc aataagtctc aaataaataa ataattaaaa aagatactta attttttttt 168780
taagtaacat gaaagtacaa agaagaaaat tgaaacttac cagattctct gtcaattgtc 168840
agtgatatta acaaacataa taatgttcac caaatgccat tggatacaga aagaatgtct 168900
ttggtcatct gtataatttt ttttccccct aagataaaaa gcacagtatt tgttttgttt 168960
tttgtttttt tgtttgcttg tttgtttttt tgtgattttt ttttttgaga cagcgtctca 169020
ctttgtcacc caggctggag tgcagtaact caatcatggc ttactgcagc cttgacctcc 169080
tgggctaaag tgatcctccc acctgagcct ccagggtagc tggaactgta ggagcgtgcc 169140
accacgccca gctaattttt aaattttttt gtagagatga tggtcccact atgttgccca 169200
ggctggtttc gaactcctga gttaaagtga ccctcctacc ttggcctccc aaagtgttgg 169260
gattacaggc atgagctacc acccctggcc tgaatatcag tatttagcat aaggtagact 169320
tttgaacatt ttataatcta gcagtgatta tcttgtagtg ttttagtaat catgctgttt 169380
actatttctg ctgttagggg ataggagtca tctatttctg atgacagtct caaagcagag 169440
aagtgtactt gtgcatgtac acaacagctg acatggatgg gaaggtggaa gagtaaacta 169500
atgccttacc tggtaccatt tgaatttatg gtaatgacat atttcaaatg gttcttatga 169560
atagaagatg attacaagcc atctcttctt gacataccag gcaactgttt cgaccccacc 169620
cacatccagc tttcagaggt gcctcaggat tctaagtctt ttagagagct tcttattgat 169680
gcctcttttg caggcagtag atatgagaaa acaaaatcct aatcactgtt ctatctatct 169740
cctatcttcc aaaatattct tgatatctcc tgtctgatgt tatctttctc tactcatgtg 169800
gatttagatc ttctttcctg ttttcagtat ttcagaagca gcaaatgcta ttttacatta 169860
taatgactgg gcaacttgat attgttttct agtcgttccc actcagtttt ctctgttaca 169920
atacatatga aagaaactac gattgatgga gaagagcttg ttaaaatcgg aaagttgaac 169980
ttggtaagca tccaccttaa tactactgtt tcactcttaa acaccttata gagcagcttg 170040
aaattttgtc cttgagacaa aatttttgtg gtcactgggt gattagcttt gtagtgggag 170100
aagaaatttg ttaattacag aaaaaattat tttgctggcg atttaataca ttatgtatcc 170160
tgtgagaatg aaagtctttg aatccaaatc caatagactc actttttatt tttattttta 170220
aaattaaagg ttgatcttgc aggaagtgaa aacattggcc gttctggagc tgttgataag 170280
agagctcggg aagctggaaa tataaatcaa tccctgttga ctttgggaag ggtcattact 170340
gcccttgtag aaagaacacc tcatgttcct tatcgagaat ctaaactaac tagaatcctc 170400
caggattctc ttggagggcg tacaagaaca tctataattg caacaatttc tcctgcatct 170460
ctcaatcttg aggtaagccc tttgaaagga agctgcaagt gtagtagctg taattcttat 170520
ttggctatta tatattttaa aagttcattt actaggatgg acacagtgac tcacacctgt 170580
aaacccagca ctttggaagt ccaaggtggg cggatcactt gagcttagga gtgcctgggc 170640
aacatgccga aaccctgtct ctaccaaaaa tacaaaaaat tagctgggtt tggtggtgta 170700
caactgtggt cccagctact tggggggctg aggtgggagg atcacttaag cctgggaggc 170760
agaagttgca ttaagctgag atcatgcaac tacactccag cctgggtggc agagggagac 170820
cccatctcaa aaaaaaaaaa gtatgtgtat aaaaaaaaag aaaagtatgt gtatacacac 170880
acacacacac acacacacac acacacacac acacacacat atagtaggga aaaaaagttc 170940
atttagtagc ttcatttttt tttttttgag acaaatccca ctcttgtccc ccagactgga 171000
gtgcgatgac gcgatctcgg ctcactgcaa cctccacctc ccaggttcaa gcgattctcc 171060
tgcctcagcc tcccaagtag ctgggattac aggcacctgc caccacgccc acctaatttt 171120
tgtattttta gtagagacgt ggtttcacct tgttggccag gctggtctca aacccctgac 171180
ctcaggtgag ccgcccgcct ttgtctccca aagttctagg attacacgcg tgtgccacta 171240
ctcagcctag cttcgttcat tctatgctat aatgtaaaag aatctggaca ttgcatatga 171300
atatatacag gaggacactc ctgaagaagt tatctttttc cttcctggca gagtttttaa 171360
ccttaaaaag ccagtttctt aatggctttt tccacacagt cttcaaagaa aattgctgtg 171420
gtcattagca gtgggtggtg tatggagatt taattgagga cttagaagca ggccaagtga 171480
atgctcgcta gtgtggtaga ggctgcttag agaacactga agatggcgtt ggatgtgtga 171540
gaacagagag gaaaaccaag aaaagtaaca aagatggtaa aatgtacgct tattttattg 171600
ctatcatctg ccttaagtgg aaattttatt tatttattaa tttttttact tttagaggta 171660
gagtctcata ctgttgccaa ggccgcagta cagtagcatg atcatggctc actgcaactt 171720
aaattcctgg actcaagtga ttcccccaac cacagcctcc tcctgagtag ctagtactac 171780
aagtgtgagc caccaggcct ggctaagttt tgttttgttt tgttttaaat agagacagag 171840
gtctcactat gttgcccagg ctggtcttga actcctgggc tcaagggatc ctcctacctc 171900
agcctcccaa aatgctgcga ttataggcat gagccacctc acttgaccta aatggatttt 171960
aaaaagcttt tttaggccag gcacggtggc ttacgcctgt aatcccagca ctttgggagg 172020
ctgaggtgag tggatcatct gagctcagga gttcaagacc agcctgagca acatggtgaa 172080
accccatctc tactaaaaaa tacaaaaaat tagctgggca tggtggtgcg cgcctgtaat 172140
cccaactact caggaggctg aggcgggaga gttgcttgaa cccaggaggt ggagattgta 172200
gtgagccgag attgcgccat tgcactcaag cctgggtgac agagtgagac tctgtctcaa 172260
aaaaacaaaa aagctttttt aaggtgtcca actgcccctt cattaaaaaa aaatctttgt 172320
tgagatttaa ttcacatacc ataaaattca ctgatttaaa gtatattaat ttaataattc 172380
tagtatattt acagagttgt ccaaccatca ccaaaatcta agttttgaac attttcataa 172440
cctcagaaag aaagcctgta cccattgaaa ttacttttcc atttgcccca ctcccatcgc 172500
tactgctttt tgcatctata tatttgccta ttctgggtat ttcatataaa tggaattacg 172560
taataggtag ttttttgtga ctggcttctt tcacttagca taatgttttc aaggttcatc 172620
tgtgttgtac cagcaatact ttattccttt ttacaggtga atattattct atagtatgga 172680
tatgggattt ttttgttttt tttttttttt gagatggagt ctcgctctgt tgcctaggct 172740
ggagtgcaat ggtgtggttt tgactcactg cagtcttcgc cacccgggtt caagtgattc 172800
tcctgcctca gcctcctgag tagctgggat tacaggcgcc accaccatgc ctggctaatt 172860
tttgtatttg tggtggagac agggtttcac catgttggcc aggctggtct cgaactcttg 172920
acctcatgat tcacccgccc cggccttcca aagtgctggg attacaggtg tgagccactg 172980
tgcccggctg atataggaca ttttgtttat tcatcagttg gtagattgat tgagctttgt 173040
ggtttttttg ttttgttttg tttttttgtt tttttttttt gagacaaggt ccctctctgt 173100
tggctggagt gcagtggcac attcactgta acctcaacct cctggcctta agtgatcctc 173160
ccaccttaga ctcccaagta ggtgggacta taggcacata ctactatgcc cagctaatat 173220
ttatttattt attgtagaaa caggatctcc ctatgttgcc aaggctggtc tcgaattcct 173280
gggctcaagt gaccctcatg ccttggcctc caaagtgctg gaattacaaa tgtgagccat 173340
cattgagttt aagaatagtc taaaggaaat tatcctaagg gtcgagactc tgaaaattga 173400
agagaaggga aaaaaggatt gaacaacttc ctttttaaag gttgggcata gtggtcttaa 173460
tgactagatt ttaaaattag atataactat aaaatattac ttgtaagtta ttatataaca 173520
tattttagat aacagaacta cattattctc acaatatctt cagtaattga cctttccttt 173580
ccatgacagg aaactctgag tacattggaa tatgctcata gagcaaagaa catattgaat 173640
aagcctgaag tgaatcagaa actcaccaaa aaagctctta ttaaggtaac tgtgaatttt 173700
tgtagagtaa tgtaatcttg tttgacaaat gtgaaaataa gaaactgaag tgggagataa 173760
tagttaaaca agatttgtta aattgcccat ggaaggcttt ttatatagtg atttaaacta 173820
aatgtcttac atgttaacat atttttttct aatgctagta tgttgacttt accaacttta 173880
tcaactgagt tggtactctt agcaaaattt tcatttattt acatttacaa aatttattta 173940
tttgcatttt tgaggtataa tttacatgcc ataacatcca cctaatgtaa gcatacaatt 174000
caatgattta tagtgcattt acagagttgt ggagtataac cacgatctag ttttagaaaa 174060
tttgttatca ctatccagtt tccctttgct cctttacaat catgtggcca ctgacctgct 174120
ttctgtctac agatttgcct tttctggacg tttcctataa atggaatcat gtaatatttg 174180
gtcttttgca tctagtttct tttgcctagc ataaccattt ggggttccac ttataacatg 174240
tatcagtagt ttatttcttt ttattgctga atagtattca attatatgct gataatatga 174300
catttggatc atttccacta atgccattgt gaacatttct gtacatgtct ttgtgttgat 174360
gtgttttcat ttcttttggg tagatatcta gggatttaat ttctgggttg tatagtaagt 174420
ttatgctcta agaaactttt ccatgtagct gtaccacttt gtatttccta cagcagttta 174480
tgaggtctgc aatttctcca cctcctcttt aacacttgtt atggtcggtc tttttaattt 174540
taaccattct aaggagtata aaatggtact tcagtacggt ttttttgttt ttgtttttgt 174600
tttttttttt gagatgaagt ctcgctcttt cacccaggct ggagtgcagt ggcacgatct 174660
tgactcagtg caacctccgc ctcccaggtt caagtgattc ttcgacctca gtctcccgag 174720
tagctgggac tacaggtgtg caccaccacg tctagctaat ttttgtactt ttagtagaga 174780
tggggtttca ccatgttggc caggctggtc ttgaactcct gacctcgtaa tctgcccgcc 174840
tcagcctccc aaaatgctgg gatcacaggc gtgagccaat acgcctggcc ccaatatggt 174900
tttaattagc atttccctaa tgactaatga tgttgaacat cttttcttgt gcttattatc 174960
tatttgttta tcttttttgg tgaaatgtct attcaaatgc tttgcccaat tttaattggt 175020
ttgtcttatt aagttgtaag gagttcatgt agtctagata caagccctta atgagatatg 175080
atttgcaaat atttcctccc agtctggctt tactttttcc tttccttgat gttttttttt 175140
tttttaaata aagtttttag tattgagatg attatagatt cacatgcagt tataagaaat 175200
aatacagaga aaacaggcca ggcacggtgg ctcatgcctg taatcccagc actttgggag 175260
gccgaggtag gcggattgct ttagatcagg agttcgagac cagcctggct aacatagtga 175320
aaccctcatc tctactaaaa atacaaaaat tagctgagcg tagtggcacg tgcctgtaat 175380
cccagttact caggaggctg agacaggaga attgcttgaa ctcgggagac aggttgcagt 175440
gagctgagat cgagccactg tactacagcc tgggagacag agtgaggctc tgtctcaaaa 175500
aaaaaaaaaa gaaaaaaagt aataatacag agaaatcctt tgtacatttt gttgaactat 175560
agaatattac cacggggata ttgatattaa taacaatcca ctaatctttc ccaattttcc 175620
ttacatgtgt atgtgtattt aattctagac agttttgtca catgtatagg ttcatgtatt 175680
taccaccaca atcaagatac tgaacagttc catcaccacg aggacccttc atattgctct 175740
tttgtaacca cttttcttcc caccatatcc ttcctttcct ggtacccagt aacctgtcct 175800
ctatcatttc aagactgtta ttgattggaa tcatacatta tgtaaacgtt tgagattgcc 175860
ttatttattt atttatttat ttattttgag atggagtctc actctgttgc ccaggctgga 175920
gtgcagtggt gcgatctcag ctcacagcaa cctccgcctc ccgggttcaa gcgattctcc 175980
ttcctcactc tcccaagtag ctgggattac aggtgtttgc caccatgtcc agataatttt 176040
ttgtattttt agtagagacg gggtttcacc atgttcgcca ggctgggtct caaactcttg 176100
accttgtgat ccacccacct tggcctccca aagtgctggg attacaggct tgagccactg 176160
cacccagcag atctttcttt tttagtacta agtagtagtc catggtgagt atgtaccata 176220
catacagttt ttgtaaccat tcacttattg aataacatat gagctaattt cagtttttct 176280
gctattacaa acaaaactgc tattgacatt catttatagg tatttatgta aacataaatt 176340
tttatttctc tgggataatt gcccaagagt gcaattgctg ggttgtataa taattgaatg 176400
tttattattt tagggaactg cctgtttttc aaattggctg tatcatttta cagtgtatga 176460
gtgatctgat ttcttcacat cctcaccagc atttggtggt gtaacttttt tattttagtc 176520
attctgatag gtgtggtagg tgatagatat ctcattgtgg tttttaactt gaatttttct 176580
aaaggctaat gatgttgagt gtctttttaa tgtgcttatt tgatgtttat atatttatgt 176640
atatatagca tatacatata ttgcatattt atatataaca tatacatgta tatatatatt 176700
tatttcccat tataatttat ttggggaaat atctgtatat ttgtcctgta gagttttacc 176760
atagtatctt ttgacgtgtt cctctgttct ttgtattttc tttgtaaatc ggtagctgaa 176820
tcttgaggct tgattaaatt caagttttgt cttatttatt tttggcaaaa ctaattcata 176880
agcagtagtg tcttcttcca tttagaagta tgtaatgtct ggttctttgt cttttttttt 176940
tttttttttt tgagacggag tctcgctctg ttgcccaggc tagagtgcag tggtgccatc 177000
tcggctcact gcaagcttca cctcctgggt tcacaccatt ctcctgcctt agcctcctga 177060
gtagctggga ctacaggcgc ctgtcaccac gcccggctaa ttttttgtat ttttagtaga 177120
gacagggttt caccatgtta gccaggatag tcttgatctc ctgaccttgt gatccgcccg 177180
cctcggcctc ccaaagtgct gggattacag gcgtgagcca ccgcgtccgg ccagaggtat 177240
agttcttata ggaaaggcag gacacatgct tgattaattt cctttatttg ccaattttga 177300
gaataatgag ttggtttcct aggtttctta gtgtcattat aaactcctag atttaaacta 177360
tttgtgttaa cccattgtag ttgttctcct caccgatgct cagattggct catcttaggc 177420
caggggtatg ttagtctgtt ttcatgctgc tgataaagac atacccgaga ctgggcaatt 177480
tacaaaagaa agaggtttat tggacttata gttccacatg tctggggagg cctcataatc 177540
atggcggaag gcaaggacga gcaagtcaca tcttacgtgg gtggcagcag gcaaagagag 177600
agagcttgtg tggagaaact cctgtttgta aaaccatcag atctcgtgag acccattccc 177660
tgtaatgaga acagcatgga aaagatccgc ccctacgatt cagtcatctc ccaccaggtc 177720
cctcccgcaa cgcgtgggaa ttatgggagc tacaagatga gatttaggtg gggacacaga 177780
gccaaaccat attaaggggt tacatcttca agtggcttac tgagtccttt tgattaacct 177840
agtaggcttt gcttagctta tttccttgtc ttatttgaca agatgttccg tattcatctt 177900
gaatattttc tgcctcagtc ctggaatcag atgcttttat aaggaatcct ggttcatttt 177960
agtgtgaatt actcctacca acctgggtac tggaggttgt ggtttttctt gggaagtcca 178020
tatttctaga atgagtgtat ttaaaaagga gctttgaaag actttatttc taaacaaatt 178080
aatattgatt aaaaagtatg gttataactt tttatcatac ttctttaagt tttaaaagac 178140
ataaaaaggc taactttaca ttttatttgt tgcatgtcct tcccaaactg aatgaaaaaa 178200
gtactaaact gacacctaca acattcctct tgtgtaggag tatacggagg agatagaacg 178260
tttaaaacga gatcttgctg cagcccgtga gaaaaatgga gtgtatattt ctgaagaaaa 178320
ttttaggtaa gcccttggct atggagttaa tttccaagaa taagcatttc tgataacagg 178380
ctatttgaag taaaacttat gtagcagtaa gtaaaatctt tatatccagt gccgataaat 178440
acttcatttt gtgtgtgtgt gtgttttctt ttgagacaag gtctcgcact gtcacccaga 178500
ctggagcaca gtggcacaat cttggctcac tacgtcctca gtctcctggg ctcaagcgat 178560
cctcctgcct cagcctccca agtagctggg attataggca tgagccacca caccctgcta 178620
atttttgcat ttttttgtag agacagggtt tcaccatgct gcctaggctt ctattttgtt 178680
ttgacattaa caagtagcta tcaaacactt tttaaaaatc ttttactaac ttttaatttt 178740
taaatcatta attcatgtga agtttcaaga agagtacaag agaggtttca tgtattcttc 178800
acccagtctt cctcagtggt tatctcttaa ataattatag tacaaggctg gatgtggtgg 178860
ctcacacctg tgaatcccag cactttggga ggccaaggca ggcagatcac gtgaggttgg 178920
gagtttgaga ttagtctgac caacatggag aaaccccatc actactaaaa acacaaaatt 178980
accctgtgtg gtggtacatg cccgtaatca tagctactcc agaggctgag gcaggagaat 179040
tgcttgaagc tgggaggcgg aggttgtggt gagccaagat cgcgccattg cactccagcc 179100
tgggcaacaa gagtgaaact ctgtctcaaa aataagtaaa taaaataata gataaataaa 179160
aaataggcgc gataaataaa aaataggctg ggcgccgtgg ctcacgcctg taatcccagc 179220
actttgggag gctgaggcag gcagatcacc tgaggtcagg agttcgagac cagcctggcc 179280
aacatggtga aaccccgtct ctactaaaaa tactaaaatt agccaggcat ggtggcaggt 179340
gcttgtaatc ccagctactc gggaggctga ggcaggagaa tcacttgaac ctgggaggca 179400
gaggttgcag tgagctgaga tagcagcatt gcactccagc ctggggaaca agagcgagac 179460
ttcatctcaa aaaagaaaaa ggaaaaaata ataataaaat aaataaaaaa taattatagt 179520
acaatatcaa agctgggaag ttgaccttga tacaatatgt gtattagttt gttcttatac 179580
tactatagag aaccacctga gactgggtaa tttataaaga aaagaggttt aattggctca 179640
cagttccata ggctgtacag gaggcatggc tggggaggct tcaggaaact tacaatcctg 179700
gtagaagagc taaggagaag caagcacata ttcacatggc ggcaggagag agaaagtgaa 179760
gaggaaagca ctgcacactt ttaaacaacc agatcttgtg agaactcatt cactatcatg 179820
agaacagcaa gggggaagtc catctttatc actcggttat ctcccatcag gtccctcctc 179880
caacatgtgg ggattataat tcaacatgag atttcagtgg ggacagagaa ccagaccata 179940
tcaagatgtg tatatagtag ttctatgcca ttttgtcact tgtatagatt tgtgtaacca 180000
ccactgcaat caagatacag aactatccta tcatcacaag gatctttctt gctaattcac 180060
tgtagtcaca ctcacctcat cttttccatg attcctaacc cctggcaacc actaatctgt 180120
tcacttttta aagccctgga gtaatttgtt caaaggaaag cttttattga ggcccattgt 180180
ataaaacaac aataataaca gagaaaacaa ggggaagaag gcaagtggga tgctaaggac 180240
tataacttga aaattcctga ttgtgtttat ccttgaagat attaggaagc aagactttca 180300
cagagcattt tttaaaagtt aatagtgata aaagatatta gacctaataa taaccagaag 180360
cattttagta taatctttta ctgaactttt ttgtagatgt taacactcta atagtatata 180420
aatcatttaa taaacttagt tttttctgtg ttacttccaa ctgtcataat gtattccatg 180480
aatgtgtaag atgccctaga atcagaacaa tgtaagattg tgggttagtg aacagtttac 180540
catcactaat ggaggtgttc tttttttgat gctttagaag taaaaaataa ttggtgaggc 180600
actcaatcct ggcctgtagt ctttagaaat gatattgatt attggaggct ttcatctttc 180660
tgattttatt tttgaactta agaagtaact ttggttttca tttgtttagt cccatgattg 180720
aaaatatggt gtttgctctc ttttttttta acttttattt tagtttcagg agtacatgtg 180780
cagatttgtt ctatagatat attgcatgta acaggagttg gtgtacatat tattttgtca 180840
cccagataat aaccatagaa cccgatggat agcttttcaa tccttgctct cctcttaccc 180900
tccaccctca aagaggccca ggtgactatt gttcccttcc tcatgtccat gtctgctcag 180960
ggtttagctc ctacttataa gtgagaacgt ttggtgtttg gttttctgtt cctatgttag 181020
tttgtttagg acaatggcat ccagctccat ccatgttgct gcaaagaaca tgatctcatt 181080
cttttttttt tttttttttt ttcgagacag tcttgctctg tcacccaggc tggagtatag 181140
tggtgtgatc tcggctcact gcaacctctg cctcccaggt tcaagtgatt ctcctacccc 181200
agctgcccga gtagctggga ttacaagcac ctgccaccat gcccagcaaa tttttttttt 181260
tttaagtaga gatggggttt caccatgcac catgttggcc aggctggtct tgaattcctg 181320
gcctcaagtg atcaactcac cttggtatcc tggcgtactg ggattacagg catgtgccac 181380
tgcacccagc catcttgctc ttttttatgc ctgtgtagta ttccatggtg tatatgtacc 181440
acattttctt tatccagtct gctgtggatg gatagctagg ttgattccac gtctttgctg 181500
ctgtgaatag tgctatgatg aacatatgtg tgcatgtgtc tttatggtag aacaatttat 181560
attcctttgg gtatataccc agtaatggga ttgctggctc aaatagtatt tctgtgtgtg 181620
tgtggttttt tttttttttt gagatggagt cttgctctgt tgtccagggt ggactgcagt 181680
ggcacaatct cggctcactg caaactctgc cccccaggtt caagcaagtc tcctacctcc 181740
gcctcccaag tatctgggat tataggcacc caccaccgca cctggctaat ttttgtattt 181800
ttagtagaga tggggtttca ccatgttggc caggctggtc tcgaactcct gacctcaagt 181860
gatctgccca cctcggcctc ccaaagtgct ggattacagg tgtgagccac catgccctgc 181920
cggtatttct gttttaagtt ctttgagaag tcgccaaact actttccata atggctgaac 181980
taattttcat tagtagcata taagcgttcc cttttctcca caactttatc accatgtgtt 182040
attttttgac tttttaataa tagccattct gactggtgag atggtttctc attgtggttt 182100
tgatttgcat ttctctaaca attaatggtg ttaaacatag tttcatatgc ttcttagcca 182160
catatatgtc ttcttttgaa aaatgtccac atcatttgcc cacttttttt tttttttttg 182220
agacacagtt tcactgttgc ccaggctgga gtgcagtgtg gcacgatctc agctcacttc 182280
aacctccacc tcctgggttc aagcgattct cctgcctcag cctccgaagt agctgggatt 182340
acaggtgcct gccaccatgc ccggctaatt tttgtatttt tagtagagat gggatttcac 182400
catgttggcc aggctggtct tgaattcctg acctcaagtg atctgcccac ctccgcctcc 182460
caaactgctg ggattacagg tgttagctac cgtgccccgc tgggtgtata tgattttata 182520
cttagaaaac cccatagtct ctgtccataa gctcctagat ctgatcaaca atttaagcag 182580
agtttctgga tacacaatca ttgtactaaa atcagtagca ttcctatata ccaataatgt 182640
ccaagctgag tgccaaacaa gaatgcaatt ccattcacaa tagccacaaa aacagtaaaa 182700
tacctaggaa tacaactaac cagagaggtg aaggatctct acggtaagaa ttataaaaca 182760
ctgctgaaag aaatcagagt tgacactaac aaatggaaaa actttccatg ctcatggata 182820
agaagaatca atattgttaa aatggccata ccacccaaag ctatttacag attaaatgct 182880
gttcctctca aactaccaat gacattcttc acagaaaaaa ctattgtaaa attcatgtgg 182940
aactggaaaa gagcccaaat agccaaagca gtcctaagca aaaagaacaa agctggaggc 183000
atcgcattac ctgacttcaa actatactac agggctacag taaccaaaac atcatggtac 183060
tggtacaaaa acagacacag accaatggaa ccgaatagag agcccagaaa taaagccaca 183120
cacctacagc catctgatct tcgacagaac atgcaatggg gataaaactg cctgttcaat 183180
aaatggtgct gggataactg cctatccatg tgcagaatat tgaaactgga ccccttcctt 183240
aacgcccata tacagaaatc aactcaagat ggattaaaaa cttaaatgta aaacctaaaa 183300
ctaaaaaccc ttgtaaaaaa cctaggtgtt tgttctctaa tacacatgag gcataatctg 183360
agatagtttt gtctgaaaac gcttttggaa ttagtacagt gtcagtcaga gaagaatcac 183420
aaaaactaca gccaacattt aaaacaggat aatgctttat ttaagccaag cttaatagac 183480
attttaaaac catatcaaaa tcatctcatc catacagtaa caatgttgta ttaccccttg 183540
gcattcacta taaaaaagca tttcaaataa tcccgtttta cataaaagat ctatttctat 183600
ttattttatt ttatttttat ttttattttt gagacagagt ctcactctgt cacccaggct 183660
ggagtgcagt ggcgccatct tggctcactg caagctccgc ctcctgggtt cacgccattc 183720
tctcgcctca gcctcctgag tagctgggac tacaggtgcc cgccaccacg ccctgctaat 183780
tttgtttttg tattttagta gagacagggt ttcaccatgt tagccaggat ggtcttgatc 183840
tgctgacttc gtgatcttcc cgcctcggcc tcccaaagtg ctaggattac aggcgtgagc 183900
caccgcgcct ggctgtagaa gatccatttt taataaaaag ctaatatatttcatcaaaag 183960
actattagaa ttaactcttc tcttacagct cttttctagc ttttccttta gtcaacaata 184020
tctctagcta atacgttaag ggaatttgta ttcacggaag aatctttgtc atttaagcat 184080
aatgtgaaat agaaaattgt tggttgttat caaagaatta gatgagcaaa tacaggcatt 184140
catttctgaa actgactaat actcaagaaa tcagagaccc attaaagtgg gtttggaaga 184200
cctgtgagct ttgcgcttga gaaaagcatt ctctatttta ctttttatga cttcttttga 184260
ctttgacttc atcttctgat atttttgttg aatttagaaa tttttagttt ctaaaaactc 184320
ttttctgcca tccctgtctc tccttttgaa aaatatagca tccttttatt ttatagatct 184380
attccttatc tcagaaacat tattaatgtt attaaagttt tcttttatgt ttttctttcc 184440
tttgggatct tttttttccc tgttgtgata tctgtcattt gtatttattt tcttcaaatg 184500
tctagtgatt tttggctggc aattaagatg gattagaagc tttgtacatg ggtgaggatt 184560
atggacctgt aagttcacta tagatgaaaa agtggtgatc tcagtcttaa gtctgggctt 184620
ccatcagata tcattctttg ttggattttt tttcttttgt tctagccctc agtcttttct 184680
gagaagattt gcccagtttg cttggagatt tttctagctg ctgctatttt ttgattagag 184740
tgggtatagg gggctgagga ttccatcatt ttgtatgtag atttacactt aaatgctcat 184800
ttctagtccc taaaccttct acagtccatg atgtctagtg aaagtgaacc tggaaattct 184860
gctgcaattc ctatagacta gtggctgtca atggaagtgg tgttggggga ctggtggtgg 184920
tgggtggttt tgtcccccag aggacatttg gcagagtcca gtgacatttt ttatcatcat 184980
gacttggatg gtactgaaca tcctaaaatg tacaggacat ccccacaatg aagaattatt 185040
tgatccacta gtgctgaggc tgagaaactc tgctctagag agtaatcctt tgttctcatg 185100
agggttatgc ggtggggtga gagtggtgtt tgtgcgtgtt gcagcagcag ggggtgtaat 185160
tgctctgtat acatacttta agtctcagtt tttaacccct aatcttaccc cttccttcta 185220
agatacctga tgccttcaag tcccacattt ttccagcatt ctgtggggca tatttatatt 185280
ctgtatccct gattatagac acttaggttg cattccctcc tctctctact ttgagttata 185340
gtccgtcctc tgttagcttt ctagcttctc aaatctggaa cacacatgct tttcccctca 185400
taggtaggga ttcttagttt cagaattgag ggcaagggaa aaatatttca tttataaaac 185460
aaagacaagg aatataattt gttctttgta atttgtattt attgcttatt gacacaggta 185520
tcaagtgaca cttgggtatc aagtgatggt gataaatgtt ggaaatgagt ttgtgtagct 185580
gtcacaattg tgttagaata cattttatag gagttagaaa aaaatattaa ctgttaaact 185640
catattaaac tttattttag agtcatgagt ggaaaattaa ctgttcaaga agagcagatt 185700
gtagaattga ttgaaaaaat tggtgctgtt gaggaggagc tgaatagggt aagcacttaa 185760
aatgatattt actgttatgt gaaaagcaaa tattgaaaga aaattttaga atgaaagatc 185820
taatattttt tccttcaaga tttttttttt ttagacggag tctcgctctg tcacccaggc 185880
tggagtgcaa tagcgtgatc tcggctcact gtaacctctg cctcccaggg tcaagcgatt 185940
ctcctgcctc agcctcccga gtagctggga ttacaggtgt gcaccaccat gcctggctaa 186000
ttttttttgt atttttagta gagacagggt ttcaccatgc tggccaggct ggtcttgaac 186060
tcctgacctc atgatctgcc catctcagcc tcccaaaatg ctgggatgac aggcgtgagc 186120
cactgcaccc agctccttca gatttttttg gaaaaaaaaa aaagatttta ttttgctgac 186180
ccttatactg aaagtaagtt atatcatgat tttttactat cattaatcac atcaaaaaag 186240
ctgacagctt atattaataa aacattacaa gaaattaact gaattgtttg attttgttat 186300
tgaaactaca aaatagtgaa tgctgaagca gttttgttaa catgtttatc agatgaagga 186360
atacagatat tgggagagac tggatgttaa ataaaagtaa tataactagg gtaggcaaga 186420
ggcctgacct ttcagggcca ccctcatatt aataccttta tttatttatt tttaattatt 186480
ttttagagac agggtcttgc ttgatgccca ggctacagtg cagtggtgta atcatatctt 186540
actgcagcct caaacttctg gtctcaagca actctcctgc ctcagcctcc tgagtagctg 186600
gacttcaggc atgcactacc atgcccggct aattatttta tttttttgta gagatggagt 186660
cagtctcgct atgttgccca ggctggtctc aaactatggg gctcaagtga tctcctgcct 186720
tggcctccca gagtgctggg gttacaggtg tgaaccacct tgccaggcca cattaacact 186780
gttatattca aatccattga caatgttgaa ggaaactgaa gaattaatag tactacacca 186840
gacctattat ttaatctcaa agtgttgagt aagattacag aaagaaacag gatgacatat 186900
ttgtgttaac ctaccgggta acttcttaca acttttgcat ggaaataatt tgtttcattt 186960
ttctaatctt atgaactagc tagatatcct accagccagc tcagcgtttt ttaaattctt 187020
atatttaggt tacagagttg tttatggata ataaaaatga acttgaccag tgtaaatctg 187080
acctgcaaaa taaaacacaa gaacttgaaa ccactcaaaa acatttgcaa gaaactaaat 187140
tacaacttgt taaagaagaa tatatcacat cagctttgga aagtactgag gagaaacttc 187200
atgatgctgc cagcaaggtt tgtcccttgt gttgatttgt actcatatta agtagagaat 187260
gggtagaaaa aattttctgt gcttaagcat taaatattct gtttattcac cccaaatggt 187320
atttctgtcc atttaaaaaa cattatttta ctatttcatc catgtttttc tcactggaga 187380
tgtcgactta tgaaaaaact actcctgctc ctggagtttt gaaaatagaa cataacttag 187440
ctgggagtgg tggctcacgc ctataatccc agcattttgg gaggccaagg tgggtggatg 187500
acctgaggtc aggtgttcga gaccagcctg accaacatgg agaaacctgt ctctactaaa 187560
aatacaaaat tagccgggcg tggtggcgca tgcctgtaat cccagctact cgggaaggct 187620
gaggtggaag aatcacttga acccgggagg cagaggttgc ggtgagccga aatcacacca 187680
ttgcactcca gcctgggcaa caagagcgaa actccgtctc aaaaaaaaaa aaaaaaaaga 187740
aaatagaaca taactttata atatattttg tagacattta gaatagtgat gctgtgatgc 187800
tttttctttg tggggatgat tgaacctaat tagtcattaa gaatttagta tgttctgtcc 187860
aggcatggtg gctcacgcct gtaatcccag cactttggga ggccgaggtg ggtggattgc 187920
ttgaggttag gagttcgaga ccagcctgac caacatggta aaaccccatc tctactaaaa 187980
aaaaaaaaat acaaaaatta gccaggcgtg gtggcacatg cctataatcc catctactca 188040
ggaggctgag gcaagagaat cacttgaacc caggaggcag aggttgcagt gaaccgagat 188100
catgccactg cactccagcc tgggtaacag agcaagactc tgtttcagaa aaaaaaaaaa 188160
aaagaattta gtatgttctg atgatgaaag atgttgaaag tatttaattt tttttttttt 188220
tttttgagac ggagtctcgc tctgtcgccc aggccggact gcggactgca gtggcgcaat 188280
ctcggctcac tgcaagctcc gcttcccggg ttcacgccat tctcctgcct cagcctcccg 188340
agtagctggg actacaggcg cccgccaccg cgcccggcta attttttgta tttttagtag 188400
agacggggtt tcaccttgtt agccaggatg gtctcgatct cctgacctca tgatccaccc 188460
acctcggcct cccaaagtgc tgggattaca ggcgtgagcc accgcgcccg gcctttaatt 188520
ttttattagt tgtacttttt ttttttgaga cagactcttg ctttgtagcc caggctggag 188580
tgcagtggca tcatctcagt tcactgtaac ctttgcctcc cgggttcaag cgattctccc 188640
gcctcagcct cccaagtagc tgggattaca agcgcctgcc accacaccca gctaattttg 188700
tatttttagt agagactggg tttcaccatg ttggccaggc tggtctcaaa ctcctgacct 188760
caggtgatcc acctgcctag gcctcccaga gcgttgggat tacaggtgta agccaccacg 188820
tccggccatt aggtgtactt ctgaggaaat agtagaacat agaaggaaaa aaatttctga 188880
ggaagcataa ttattgcaat aactgaaaaa atcagttttc cttgcttgtg tagatggcta 188940
caggaaggga aataaacatt actgggcatc tggataaatt agcatgagtt aaagcatttc 189000
ttctgataca atgtctaaaa ttgacttttt ttttttgaga cagagtctct ctctgtcacc 189060
ccggctggag tggcagtggc acaatctctg gctcactgca acctccacct cccaggttca 189120
agcaattctc ctgcctcagc ctcttgagta gctggaacta caagtatgta ccaccacacc 189180
cagctaattt ttgtattttt agtagagacg gggtttcacc atgttggcca ggctggaaaa 189240
tttactagtt cttatcaaga taaatccttg tgtagatact ttcatcagat tcctttcacc 189300
gtatccattt tgtctaacac ttatttttaa aaatatagct gcttaacaca gttgaagaaa 189360
ctacaaaaga tgtatctggt ctccattcca aactggatcg taagaaggca gttgaccaac 189420
acaatgcaga agctcaggat atttttggca aaaacctgaa tagtctgttt aataatatgg 189480
aagaattaat taaggatggc agctcaaagc aaaaggccat gctagaagta cataagacct 189540
tatttggtaa gttcaggctg ttctgttcta gtcttgatgt gttaagtgta atgttgattt 189600
caaaactgat aattttgtga aacatagatg acggtgtcac caatactctc taccatgcac 189660
aaactatttg ttcagggtga agattaatgc ttttattgtc tttgaattaa aacaaatctt 189720
ttttccctcc ccaccctccc ttctgttttc tataaaatgt tacatttatt aagtaactaa 189780
gtatataaac gttagaagta gaagtcctct ttttccctga ctccggctcc tgaccctggg 189840
tcattgacca taggtgttat tgttaaattt cttgggcctt tttctggaaa ttttttgtgc 189900
ataaacattc tgcaactttt tttggggggg gcatatatat cttgatggtt tgctatattg 189960
tcagaatatg tttgtgtctc attgttttta actattatct ccctttagaa aaaagtatgt 190020
ttatttagtg tgaaatacta tcctcattat ggaaaatttg gacattcaga aatataaaaa 190080
tttaaaaaat catctagact ctagtctcac taagccaagc actatacttt ttggtattga 190140
tttccagttt tttttttctt ctgtaaactt accataattc tgtatattat ttttagttag 190200
agattaattg tagaatattc taattctttg tgtactatga attatagtta tgggtctgat 190260
acacttacaa atatgttaag ggcttacaga gcactataga aaattgtaat gtatatttaa 190320
atatttcctt gctttgtagt gagccagttc ttttaagctc gttattacaa attctacaac 190380
aagggtatac ttttgtcaac ttctttgaaa tggtttgaac ggtatttaat atatttatgt 190440
caaagtttac atctttctgt ttttgtttgt tataggtaat ctgctgtctt ccagtgtctc 190500
tgcattagat accattacta cagtagcact tggatctctc acatctattc cagaaaatgt 190560
gtctactcat gtttctcaga tttttaatat gatactaaaa gaacaatcat tagcagcaga 190620
aagtaaaact gtactacagg aattgattgt tagtacatcc tttaaaatat ttttgaaggg 190680
ttgcatttga taagtatttg ataaaatatt ttgaagggtt acatttgata agtctttata 190740
aacaatgtta actgctattc tttcttcctg agctttacta gacacagtca tagacacgtc 190800
actgtgagag actacatata tatatatttt gttttgtttt gttttgtttg tttgttttga 190860
gatggagtct cgctctgtcg tccaggagtg cagtggcgtg atctcggctc actgcaagct 190920
ctacctcccg ggttcatgcc attctcctgc cttagcctcc cgagtagctg ggactacagg 190980
cgcccaccac cacacccggc taattttttg tatttttagt agagatgggg tttcaccgtg 191040
ttagccagga tggtcttgat ctcctgacct tgtgatccac ccgcctcggc ctcccaaagt 191100
gctgggatta caggcatgag ccaccgtgcc cagccagcct ctccttttct ttatttgtat 191160
ttatttattt ttttcagcct ctccttttct cggctcactg caacctctgc ctcctgggtt 191220
caagcagttc tcttgcctca gcctcccggg tagatggaat tataggcatg tgccaccacg 191280
cctggctaat tttcgtattt ttttttagta gagatggtgt tttaccatgt tggtcaggct 191340
ggtctcaagc tcctaacctc aagcaatcca cccacctcgg cctcccaaag cgctgggatt 191400
acaggcgtga gccaccgtgc ccggccgaga gtacgtttat atttttaaag acagatctct 191460
ccactgttta ttctctccca gaaagattat tttcaaatgt atggaactaa cttttgaaac 191520
ctttttcact catgtcttgt aacattagga gtagcagtta ttagtgaggc ttctaatgac 191580
taaagggcaa gtttagcacc acatgatatc aagggacttg ttagttggcc cagaaattgg 191640
caagtcactc tttccccagg ggtcctggac ccaaccagaa ggggatattg ggtagctgat 191700
tttaaaacta cagtaatata tgatagtaat aatggtgcaa gaaaaatatc ttagaattct 191760
ggggacacat atcacttctt agggttagat ctgtgtggcc ccttctgggc cataactata 191820
aatcttttct ccagagttct atggaagtca ctcatctaat tgcacttaat attacctcct 191880
tcatacttga tttatatata gtctttattt tataattgta tggttggtct aggaagttct 191940
tagccatata atttatttgt tttctttgtg cagagtcttt gcctcccttt ttcagcttaa 192000
caatatttat taaacatttt ccaagtaaat actacaaatg ttagctgacc ctctgcctca 192060
attcagtgct tagatgacat tatttgatag gttttctcaa tcacccaaat ttgacaaaat 192120
taaaagatat cctgtcagta ctaacttttc aaatattgat tcattcatta aatggtggtc 192180
tgtatatata taggcatact tcagagatat tgcaggtttg cttccagacc actgcaataa 192240
agtgaatatt acaataaagc aagtcatgaa ttttttgctt tcccggtgca tataaaggtt 192300
atgttgacca ggcgcagtgg ctcacacctg taattccagc acacgcctgg tgggacaatc 192360
agaacacaac gtttatcagt tacatttgct gtcttatagg ggtacagttt atggtacccc 192420
aagacaatta caatagtaac atcaaagatc attgatcaca gtgtataatg aaaaagttag 192480
aaatactgta gtagttacca acatgtgatg cagagacaga aagtgagcac atgatatttg 192540
aaaaaagtca ctgatagacg tgctcaaggt agggttgcca caaaccttca atttgtaaaa 192600
atttggtatc tgtgacacat agtaaggtga agcacaataa aataagctat gcctgaattg 192660
atacatttct acttaccaag ttacaatttt tgcttgaatt aaaagaaaag aggcatgttg 192720
ctctcacaaa attagttgaa attggtatgc tagctcttct ttccaaaaga atgtcagtag 192780
acctataagt atttactaat gtatttctgt tatacttcct cagccctatc ctaccaaaag 192840
gagattagat caggattttt tttcttttta taaatatttc caatctatac tacattctta 192900
atttccctat ttcttgacaa gaacaacatc tttcacaagt tcttctgata caataggatg 192960
taagtcatct cagatcttca aagttaagta cttccagccc aagggctaat cttgatgact 193020
acttggtctc agcctttttg actggtaacc taaacttgtt tgaaatttat tttcttaaaa 193080
tatacctgta ggtttttaaa aatttatttt gtttggagtg ctgaaatctt attaactgtc 193140
attttcctct tttgaattct tctgacttct atttcattaa actattaaat agttctggct 193200
gggtgcggtg gctcatctca gcactttggg aggccaaggt gggtggatca cttgaggtca 193260
ggagttcgag accagcctgg ccaacatggt gaaaccctgt ctttactgaa atacaaacat 193320
tagctaggcg tggtagcagg tgcctgtaat cccagctact tgggaggctg aggcaggtga 193380
atcgcttgaa cccaggaggc ggaggttgca gtgagccaag atcacgccat tgcactccag 193440
cctgggccac gagagtgaaa ctatatctca aaaaaagaaa aaaagaaatt ctgtgttttc 193500
actgggcttg aaaaaagaga aattattata tttattgtaa tataataaat tattgtatta 193560
ttgtattgat ttatctatgt agagtataaa aaatggagaa tggggccggg cgcggtggct 193620
cacgcctgta atctcagcac tttgggaggc cacggggggc ggatcacctg aggtcaggag 193680
ttccagacca gcctggccaa catggcgaaa ccccatctct actaaaaata caaaaattag 193740
ctgggtgtgg tggctcacac ctataatccc agctactccg gaggctgagg caggagaatc 193800
acttgaaccc aggaggcaga ggttgcagtg agccaagatg ttgccactgt actccaacct 193860
gggtgacaga atgagactcc gtctcaaaaa aaaaaaaaaa aaaaaagaat ggagaatgga 193920
aatgtaaatt ttaatgtgaa tgtttagcta ccaaagtatt taagatatca tttagaaagg 193980
tttacagaag tggaaatatt ctttttaaag acctatttgt ttatttctga aaccagaatg 194040
tactcaagac tgatcttcta agttcactgg aaatgatttt atccccaact gtggtgtcta 194100
tactgaaaat caatagtcaa ctaaagcata ttttcaagac ttcattgaca gtggccgata 194160
aggtaacaaa tgctatgttc ttaatatctc aaaattgatg tgttgtttaa gaaggaaact 194220
catttttgtt tcttcaaaga tagaagatca aaaaaaggaa ctagatggct ttctcagtat 194280
actgtgtaac aatctacatg aactacaaga aaataccatt tgttccttgg ttgagtcaca 194340
aaagcaatgt ggaaacctaa ctgaagacct gaagacaata aagcagaccc attcccaggt 194400
atgttgttta gcggacttgg ggagtacaga aagagagttt taggatgatt tgatatgact 194460
tgataattaa tctatgttac acaatctgaa tactgtaaaa gctgaaacct gaaaatacca 194520
tagccactgt tgcttataac agtaattatt gtagaacaat tgagaatact tctcttaata 194580
tttgaagttt tgctacatct agaaccccat gcagaaccac aatatgacaa aacagtcctt 194640
ttctcacatc aagatgaaag atgaatctgg aaaaacatac ctttagagaa gaatggttat 194700
aacatttaaa gtgaaaatgt atctacatta aaacctgcta agttgtttct aggatggcat 194760
ggatagttgt ctttcataaa ccaagtccta ctttctctta tttctgtctc actgatagac 194820
atttaaaaca tagtaaatcg atacaacttt taattcttat tgattataaa tgtaattcat 194880
gatttatctt ccctgtaaac tgttcctcat tatatgaggc tttaaaccaa aaccaagcct 194940
tcaaaccata atctgtaaat atcagatatc tgaaaaacag cttctggtat tcttaagact 195000
ttaataatga ctgtctaaag ttttattaaa tgagcttatt ataatatgac agaactctta 195060
taatagttaa catttattga gcaattcact gtgtttattc ctcccatcaa tatagatata 195120
aattctatta ctagtcagat ttttaatgag gaaactgaga ccctgtgagc atctaatagt 195180
atgtagatca tcttgcagaa ggtggtagtg atcatactac ctgaaaagca tccatgtttg 195240
agtggctctt ttgtgtgttt tttggcaact taaaactgca gcattttctc atacatctac 195300
atagggtatt tcccttaaac ccgttgagaa ctttttaggt gtatattcta aggctgatcc 195360
cctttttata aatttgctgt tttgaaatgc ttaaaattgt tagacagctc tttaaaaaaa 195420
caatacaaaa aaatctgatc tgaaaagtat cttagcatga atggtttggc tttcctggct 195480
ttaaggaagc aagttcagta tgtgagctat ttcttaggtt ttccagaact tgaaatgagc 195540
actactaaaa taattatgta aaactttgaa cacatttaca tatagataaa tataatacct 195600
gcattagcat tcaaattatt aatctgataa tacctttgag actagtaaaa atactgacag 195660
actttattca taatcagaat gttagatatg tattgtcaac tgatgtgtta ttcagagata 195720
ctggccaacc agcagactga agagtgaata agaatgttgg atgcattttc agctcttctt 195780
tggtcattag gatggcttct tcattttgca atgtagcatg ttatatgcct ataagagctt 195840
gttttcaaag atgtaaaata tgagcaaaga taagactatt tacattttgt taatatgatc 195900
caactaggtt ctgtaacatt ttttagctcc agtaattgat aatatttttg gattgcttga 195960
cccgttagta tatcacatta attttccctt ctagcagtgt tatcagttaa aaagcaacta 196020
tatatatctg tcaaagtggg tcttaaacat aatgtgatat atgggttgtt tgattagttt 196080
tgaggtacac tgaaagtaat ttgttactta cagctaaaat agtaaagttt gctaattatt 196140
tactttttaa aaaatcctgg ctggatgtga tggctcatgt ctgtaatctc agcactttga 196200
gaggctgagg cagggtgatc acttgaggct aggagttcaa gaccagcctg gtcaacatag 196260
cgagatcacg tctctacaaa aaaaatttgt ttaaattagc caggcatggt ggctcatgcc 196320
tgtgtagtcc caactacttg gaggctgagg aaggaggatc ccttgagccc aggaggttga 196380
agctgcagtg agccttgatt gtgccactgc actacagctt ggacaacaga gtgagaccct 196440
gtctctaaaa aaaaaaacaa atcctacatt ttaatatcac ttccactgct ttcctctgta 196500
gaagaaaggt aaagttaact ttatctcttg tcaagaattt atataattct cacctatgga 196560
caatacttct tgttttgtta tcagtcataa aaaatgttca agtgtcataa ttttaagtct 196620
cttcacttcc cacacctttc ttacaggaac tttgcaagtt aatgaatctt tggacagaga 196680
gattctgtgc tttggaggaa aagtgtgaaa atatacagaa accacttagt agtgtccagg 196740
aaaatataca gcagtaagct atttttaaat tctcttaaac ttttctgtaa gtctgaaatt 196800
atttaagaag aaaaagcttt aaatagtaca aataattcct ctgtgtactt tcaaatttct 196860
cttttgttaa tattttatta tgtatgtgtg tatgtatata tatacatgca tataaatgtc 196920
ttttcattgc gtatttgtgc tctcttttaa gacattgaaa aacctggctg ttacccacaa 196980
tatattttcg aatttcctca atcttagaaa acacactaag taatttcaca atttctaacc 197040
tatattactg atgaaaaata tactaactag agcagggttt ggcaaactgt ggctagcagg 197100
ctggatgcct gttattgcaa ataatttcat tggacactgt ttacatgttg tctttggctg 197160
cctttgcact gcagtggcaa agttgagtca ttgcatccag gtcattgaac aatagcctaa 197220
aatatttgct atctggcttt tcaagaaaaa gtttgctgat tcctgtatta gggttttgtt 197280
ttttgttttt gtttttgttt ttgcgacgga gtcttgctct gtcacccagg ctggagtgca 197340
gtggagtgat ctcagctcac tgcaagctcc gccttccagg ctcacgccat tctcctgcct 197400
cagcctcccg agtagctggg actacagatg cccgccacca tgcttggcta attttttgta 197460
tttttagtag agaggggttt caccatgtta gccaggatgg tctcgatctc ctgacctcgt 197520
gatccgcccg cctcggcctc ccaaagtgct gggattgcag gcgtgagcca ccacgcccgg 197580
ctctgttttg ttttttcaga caaaaacaaa cagcctgctc tgtagcccag gctgaagtac 197640
agtggcgcga gtgcagtggt gtgatcttgg ctcactgcaa cctctgcctc ctgagttcaa 197700
gtgattctcc tgcctcagcc tcccgagtag ctgggattac aggcacatac caccatgcct 197760
ggctaatttt ttgtattttt agtagagatg gggtttcgcc acgttggcca ggctgatctt 197820
gaactcctgg tctcaactga tcctcctacc tcggcctccc aaagtgctgg gattgcaggt 197880
gtgagtcact gctcccagcc tgtattagag tttaatgttg ctttgtagcc tcattgcttg 197940
ccagttccgc atacttaaca tacactacag tcagacctat gttttctttt aggaatttta 198000
taactttatg gtttaacatt tatattcatg atccatttaa aaatttttat aaaacatagg 198060
agatttgtca agtttcagtt ttttgcctat tgagtattct atactcatga ggagaaagat 198120
actatattta ccctattttt ccccatttaa ttctgaaatt aaacttccta aagtttcaag 198180
ctttctttgg tgggaagtag tctttaaggg tgggtctgct ggacagaaat tgtttttctt 198240
tgtctaagaa tgtcttgatt tccccttcat tcctgaaagg tattttcact ggctgtggaa 198300
ttcagggttg acaattattt ttttccagca tttgaaatat ttccattttc ttctggcctc 198360
tatgaaatga gaaatccact gccattcaag taattgttcc cttataggca gtctccctat 198420
agctgctttc aaaacttttt tgtctttaga aacttgatta tattttgtct acttatattt 198480
cgttgagttt attctatttg ggttttgttt agcttcttga atctgtaggc ttatgccttt 198540
caccaaaact tgggaaattt tcatctatta attctttgaa tatttttcag ccctatactc 198600
tttttcatct gcccctatga ctttgatgac acaaatgtta gatcttttat tttggtccct 198660
caggagatat gtagaatata tttcttatag atacatatat tctctttgtt gttcacattg 198720
gggaaattct attgatctgt cttcatattc actgagtctc cttctgtcat ctctaaccta 198780
ctgttaaacc cattcaatga ggtgtttttt cagttattgc ttttttattt ttagttttat 198840
aattttcatt tgcttttttt ataccttgtt tcttagctag gatttttttc ttttccagga 198900
gtattttaat ttctttttgg agcatttttt gatggctgct ttaaaatcct tgccagacaa 198960
ttccaacatc tgagttttct tgttgttgcc atctattgat tgtcttttct cattcaactt 199020
gtgatttttg tggttcttga tatatgataa gtgatttcct attgtacctt gaagattttg 199080
ggtattatgt taggagattc aggatcctat ttaagtattt tttagctggc attcaccctg 199140
tttaggctta gcatacagat ccaggctcac ttttatgggc tgtggttcca ctgacaattt 199200
agtttttaga gcccttgcag tgttattctg ataatgcttt gtttgtgtgc tacccacatg 199260
agaaaaattt gtattctgct tttgttgcat gaagtgttct gtatgtgtca ctttgatcaa 199320
gttgattgat agtgctattc aggtcatcca cggccttact gagtttctgc ctatttgttc 199380
tatcagtgac ttagagagga gtattgaaat ctctaattgt agatttatct atttttttta 199440
ttagttctat cagtttttgc ctcgtgtatc tgaaactctg ttgttgggtg catatacatt 199500
taggattatt atatctcctt ggagaattga cctctttatc attatgtaat atccactttt 199560
atttctgata agctcttgtt ctgaagtctg ttttgtctga aatgaataca ggttttctag 199620
ctttcctttg attgctattt gtatgatgta tccttctcca tctctttact tttggtctaa 199680
gtctttatat ttaaagtggg tttcttatag acagcatgta gttgggtctt gctttttcat 199740
caaatttata atctatgatg ttttattttt atttatttat ttgagacaga gtcttgctct 199800
gtggcccagg ccggagtaca gtggtgcaat cttggctcac tgcaacctct gcctcccggg 199860
ttcaagtgat tctcctgcct cagcctcctg agtagctggg gctacaggca cacaccacca 199920
tgcccagcta atttttaaat ttttactaga gatggggttt caccatgttg gccaggctgg 199980
tttcgaactc ctgacctcaa gtgatttacc tgccctggcc tcccaaagtg ctgggattat 200040
aggtgtgagg cactgcaccc agctgccttt cctcatttaa attgagcact ttttatgttt 200100
ttatctcttc cattgactta tttatacttt ttaaatattc cctttttagt agtatgtcta 200160
ggatttaaag tatacatttt aagataattt gaatttgtat acattttaag ataatttgaa 200220
tccattatca aataatttgc tgtttcatgt gtagtgaaag gacattataa tagtatattc 200280
ctgtttcctc tttcccgttt gtcattattt tcatatgttt tacttaggtg tttatatgtg 200340
caataaacac ctaatacatt gttacttgaa ttgctttatc tattttcttt tttaatgtat 200400
ctactagatt tttttttttt tttttttgag agggagtctt ggtctgtcac ccaggctgga 200460
gtgcagtggt gcaatcttgg ctcactgcaa cctctgcctc ccaggttcaa gcaattctcc 200520
tgcctcagcc tcccaagtag ctgggattac aggcacctac caccatgccc ggctaatttt 200580
tgtattttta gtagagacgg gggtttcacc atattggcca ggctagtctc aaactcctga 200640
ccttgtgatt gcccgcctca gaaattttta attgcatatg tggcttctat tatacttctg 200700
ttgaacagtg ctgttctgga actctagcta tcatttatat atccctacct aacttctgtc 200760
atttgttttt caaccctgtt tttgatcctg ttaagtatag catgttccat tgtgaactct 200820
tcctatatgc tgaatcaaaa aagtatgtcc tactttttat tcctagtatc tagactgatg 200880
gatttctagg ccattccata gccgtggagg aaatactaga gtaggattga agtaggaagg 200940
gaagtatgat atactttctt aaggcccatg caaatcattt ttcctgcttt cctgacattt 201000
attgtctaca ccagagtttc ccagtatggt ctagggactt tgtattcatc acccaggaat 201060
ctaggtgatg tttatgtaca cactgcacat aactgcgttt ggcatttatt gtcatggctt 201120
atagtacttt taactcaatc tcgtcttccc attaagttgt gagattttgt tttgtttttt 201180
ttatttgaga cagagtcttg ctctgtcgcc caggctggag tacaatgaca caatctcagc 201240
tcactgcaac ctctgcctcc tagggtcaac caattctcct gcctcactct ctcccaagta 201300
gctgagatta caggcaccca ccaccacgcc cggctaattt attgtatttt taatagagac 201360
agggcttcac catattggcc ggactggtct ctaactcctg acctcaggtg atccacctgc 201420
ctcggcctcc caaagtgctg ggattagagg tgtgagccac tatgccttgc ccttttttca 201480
gtcattgtga ctttcacgtg taggtttggt ttttaacatc tttgctgact cacttggttt 201540
acccacgtgg tgtaccatct agtggtaata gagagtcact tcattccaca aggtttaaat 201600
cttgtaagat aacttggaat gctattatag ggcaacttac cgagatggaa attatcaatg 201660
aattttaaat gtatgtctaa ccaacattaa tattatatca agtttatgtt acattgttct 201720
atatgtataa tttgtcacac cccaatataa ttttaattat tagaaagctt tatagctaat 201780
ttaaacataa catttgtaat gttcatgatt tgttttacat actaggcaag ggaaactgct 201840
taataacaga ataaatgact gattgtccaa tttagggact agacaagagg ctgtagtaga 201900
tgccctgaag taacattgcc tagtaagagg aataaaacaa gtagacagat tttgaaagca 201960
tcataactgg aggagggttg tgaaaggttt catgagcaaa gatgatcctt tcaaaggtca 202020
tttaatggca atgctgtgaa aagacagtgg ccaaggcatc cattctcaat gcagaagatt 202080
tactaagatc acgggccctg gagccagact gtccacgttc agatctgcca cttaagagct 202140
gagtgatctt gggaagttat atatcctttg agtcttaatt ccccatttca acagtgtcat 202200
tctcttttcc tataggaaat ctaaggatat agtcaacaaa atgacttttc acagtcaaaa 202260
attttgtgct gattctgatg gcttctcaca ggaactcaga aattttaacc aagaaggtac 202320
aaaattggtt gaagaatctg tgaaacactc tgataaactc aatggcaacc tggaaaaaat 202380
atctcaagag actgaacaga gatgtgaatc tctgaacaca agaacagttt atttttctga 202440
acagtgggta tcttccttaa atgaaaggga acaggaactt cacaacttat tggaggtaat 202500
aactttgtaa gtggaactta ctttggggag aataataatc agaaagttaa atattcttgg 202560
ctaagaatag atttcaaaac aaatgatatt ttaagctata atgacttaaa cttttaagta 202620
taatatttgg tatgcttaca gatgatctcg ttttgtgctt tgttatatgt cttctcaatc 202680
ttggaattaa tttaacattt gtacagtttc tttcctgtat ttatccttgg tttctgactt 202740
agtagtttct tcaaagaagg gcaccctgtc ttttgatatg taataccttc cacttatata 202800
tgcttttttt tttttttttt ttgagatgga gtctcactct gttgcccagg ctggagtgca 202860
atggcacgat ctcagctcac tgcaacctcc gcctcccaag ttcaagcgat tctcctgcct 202920
cagcctctcg agtagttggg attataggca cctgccacca cacccggcta atttttttgt 202980
atttttagta gagatggggt ttcactatat tggccaggct ggtgtcaaac tcctgacctc 203040
atgatccacc cgcctcggcc tcccaaagtg ctgggattac aggcatgagc cactgcgccc 203100
ggcctatctt tgctttttac ttttctccaa gtacattcaa acctattatt attttaagta 203160
tttaggttat tgttctggta actaaaggat acttgatagg tttatggatt tgcttgaact 203220
taacgcttgc atgagccct ttgtaacttgg ttttttcttt ctttgcatta ataggatttt 203280
actaacattc tcaggaagta ggtacaaaga attaaaattt ttaatctata taagctgttt 203340
tcactaaaag gaactagagt ttgtatacaa atagctaatt tcagatttgt gataggaaat 203400
gtataatata tgagcctaga gatcttgtca taccagagag taaggaagct gttaaagatt 203460
tctgaggttg tcaaaaggtc ttagtagcca acctccccat cttcaaaggt ggcataattt 203520
gagcatcaaa aagaataatg gccataatgg attaaaacat atcaaatata ttaaaactta 203580
cgaactcata aaaatttcat aggtctcctt tggatcatgc tagggaagca acacattgtc 203640
ctgaaaatgg gtaaagaaga agaaaaaatt tagcatccat ctgtctttcc taaaagaacc 203700
atatcttggt ttagcagggt aatgaagaaa agtgtttcat tagactctaa gctaataaaa 203760
tggagaagaa atgatagaat taatatatca ccagtatgca actcttaata aaataacccc 203820
tgtggatgtt aatgatggct taaaccattt gatcaaaagc tgttggggaa ctttataatt 203880
aataggctaa ccatacctga actcactgat aaatcttaac atgagagaca cagcaaagca 203940
ttttgtgctt gcagtactac ctatgaaata ctcttgctaa aaaattaaac ctgaattaaa 204000
tcaagccttt aggtctaacc agtttgtaga aaataaagca tagaaatgca gttgaccaaa 204060
tccagaattt gagatgttca atggggtaaa gacctcatgt cttcagcaac aagttttttt 204120
tttcaaggga aaaaatatga gaaacaataa cctgtatatc aaaagagact taagactaga 204180
ggtccttaag tcttagtttg cctgagacag tccttgttga tattgttgta ttagaatgat 204240
tattaagaat gggccctttc actccaaaag gtgtcccaat ttggatgata aattatatag 204300
tcaccgactt aagacctatg aaccaaatct agtggtgaat tctggttcaa acaaagcaat 204360
tgtgacacac atttgagacg actggtgaag tttgaacatg gactagacat ttgataggaa 204420
ggaatcagtg ttaacctttt tagatgtgat agtggtctta aagtgctctt acctcttaga 204480
gatacatgct gaagtaaatg gatgaaatga tacaggattt gtttcaaaat aatcctgggt 204540
ttatggagga gttgagtggc cagtcctgtt ggtaaatgtc tgtactccac ttgttggtaa 204600
atgttgaagc tgggtaatga atacaaggga gttaattata ctgttctctc tacttttgta 204660
tgtgttttta aatcttttat tactgaaggc caggtgtggt ggctcatgcc tgtaatcttt 204720
ggaggtcaag gtgggcagat tgcttgagcc caggagtttg agaccagctt gggcaacatg 204780
gtgaaatgcc atctcttcaa aaaatacaaa aattagccag gcatggtggt acatgcctgt 204840
agtcccagct acctgggagg ctgaggtggg aggatggctt gagcctggga ggtcaaggct 204900
gtagtgagca ctactcatgc cactgcactc cagcctgggc aacagagtga gacttgtctc 204960
caaaaaaaaa aaaaattatg gaaaaggtaa gggaaaatat gatgttgaat aaaacactgg 205020
caactttaat tttagtaata aatatttatt tgcatcattt acaggttgta agccaatgtt 205080
gtgaggcttc aagttcagac atcactgaga aatcagatgg acgtaaggca gctcatgaga 205140
aacagcataa catttttctt gatcagatga ctattgatga agataaattg atagcacaaa 205200
atctagaact taatgaaacc ataaaaattg gtttgactaa gcttaattgc tttctggaac 205260
aggatctgaa actggatatc ccaacaggta ctttaaaaga gaaatagaat tgttaaattt 205320
tttgaagtcg aattcaactc tatgtagtgt cagatgttca gaaaaattag gtcctgccat 205380
tgcctgacag aaatttaaca tctcactgta atcaactcaa aatgggaaaa ctggaacctt 205440
aaaatagttt taatcaagtg tcatgataca ggtggtatca caattcagta taatttcaca 205500
tactttcagg gagttgactt tgttaatagg ggatttttta aaacaaatac aaacctagca 205560
ctgtttgtaa aaggacattt taataccaca aatatgggag agatgtaact gactaaatct 205620
agtttaatgc aaagtttacc acattgtgca ttttgttctg tgtccctctc attttgtcat 205680
agactattgc catttaggaa tccctgtttt aagagatcag ggatcaccat ggaccattag 205740
catttaggaa tcaacatttt gagagatcac atatggcaaa gatcattata ggctgtaatg 205800
attcagggag gtcttcatgg aagagatgta acaagctgtt ttacacagca tgaataattg 205860
ggttcttcat tggattatgg taatggttgc acaccctgta aactcactaa aatccactga 205920
attgtacaca taaaatgagt gttttatggt atgtaaatta tatcacaaaa ctcttttttt 205980
tttttaattt aaaaagcaat tgttaaaagg ggatggtgct acatagaatg ttggtttatt 206040
tattccccaa gagacctgta ggatttactg tctacttttc aatgaagtta ggaatgtaaa 206100
tgttgagtga aaaggcaagt acttttgtaa atcatgagag catgagctta ttacagagaa 206160
atataactgg gggcctgatg cggtggctca cacctataat cccagcactt cgggaggcaa 206220
ggcaggagga tcgtttgagc ccaggggttc aagaccagcc tgggcaacat agtatatgta 206280
tataactagt taatatttaa ccaccacacc acatttatag tgaaatgatt actcctatca 206340
aagtaatcat gttgggaaat ctactcattc caataatgct gccatttttt tcagaacact 206400
ataactcctt ttagagctta taacacatct tttatatatc ctcagagatg gtaaacttca 206460
tgttttagag agtaggatga atcttgaaaa cagtaaagtc tgtcatcaaa ttagaaagta 206520
ttgttagttg caaacaatgt ctgacaggta gcaagactga tcctcatata tggcaattat 206580
atttttatat ttttagaaga caagattaat taggaagttt taggtttttt taaaaatatt 206640
tacatctcat actttaattt ttacctctta tctaatgtcc gttaaaggta cgacaccaca 206700
gaggaaaagt tatttatacc catcaacact ggtaagaact gaaccacgtg aacatctcct 206760
tgatcagctg aaaaggaaac agcctgagct gttaatgatg ctaaactgtt cagaaaacaa 206820
caaagaagag acaattccgg taaatttaaa ggatcatatt ttataataga actcttttat 206880
gaactcttga tgtggctgac ttcatgtgaa gaattttact gtttacccct caatcttacc 206940
ccgcctctca ttaatgatag gcctagccct taggcttgtt tcttttaatc ttactagttt 207000
ttaaattatg ctagtagata ataatagcca atacttaata ttgtgcttag taacattgtt 207060
atataggaac ctctactctc tataaaaata tattaggaat tattttcatt aatttgtaga 207120
ctttctaata attttgttga ggtttaactt ttatattata ccatgggcat tgtgttatac 207180
tcaaagctgc tgttactctt gtgatgactt ttaagccctt ggacttagtc actcatccag 207240
tttcttcttt taggatgtgg atgtagaaga ggcagttctg gggcagtata ctgaagaacc 207300
tctaagtcaa gagccatctg tagatgctgg tgtggattgt tcatcaattg gcggggttcc 207360
atttttccag gtatgtcata tcagataacc cttccacatc tgatgtaagt cattcattta 207420
ctattcatta taaaggtatt gttcgtggtg agcagaacaa caaaaacctt tcaattattc 207480
cttaggatgg cttgttaacg cttatgattt gaattattta caaaaatctg atattgataa 207540
ggggttttct gtagaataaa tgaaggcaga atttgactta atctacatcc ttagctgata 207600
atctttagct attgtattta ttccttttct gcttgctata tactcagaca ctgagagcag 207660
tcatttctct tccttctacc tttgacatgt aagtcttgga acctatctct gcccataatc 207720
agactttgaa ggcaaagtga ttgaaaagat tgagagactg gaggcctctt aggttatatt 207780
agtgatttct cctgcctgag gctcttctct tgtaaactgt tgccccatgt ttcctgagca 207840
ccttgcctgc tctaatccag tgtaattaaa tcctgtgacc tctcctaccc actcttcttt 207900
tttttttgag actgagtcac ccaggctgaa gtggagtggc acgatcttgg ctcacttaac 207960
ctccgcctcc tgagttcaag tgattctcct gactcagcct cccgggtagc taggattaca 208020
ggcatgcacc accacacctg gctaattttt ttgtattttt agtagagaca gggtttcact 208080
atgtgggcca ggctggtctc aaactcctga cctcaagtta tctaccctcc tcggcctccc 208140
aaagtgctgg gattacaggc gtgagccact gtgcccagcc ctctacccac tttttttttt 208200
cttttgagac ggagcttcgc tcttgtttcc caggctggag tgcaatggca cgatcttggc 208260
tcaccgcaac ttccacctcc tgggttcaag tgattctcct gcctcagcct cccgagtagc 208320
caggattaca ggcatgtgcc accatgcctg gctaattttg tttttttttt tttttttttt 208380
tttttttttt tttttttttt tagtagaggg ggtttctctg tgttggtcag gctggtctcg 208440
aactcccaac ttcaggtgat ctgcctgcct cggcctcccg tagtgctggg attacaggtg 208500
tgagccaccg gcccggcccc tcctacccac tttttaacac tgttgagaac atagttggtt 208560
tatgattcat ctcagcattg atgactgagt acacaatcaa tgtcaccagt cccttaatgt 208620
tctctatggg taagtaggag gattccaatg aaatacaact tccaagtgag gctctataaa 208680
gtgctggtat cttttcctct aatttgaggg tacaagccta gacagagtgt gtgaaggaaa 208740
aatttcctta cgtaggacat tggtatctac atttacagtt gaagttctac ttctgagatg 208800
catatgcttg tacctttttt tttttttttt ttttttaaat atatatagag agagggtctt 208860
gctatgttgg ccagactggt cttgaactct tggcctcaag cagccctcct acctcagcct 208920
cccaaaatgc taggattaca ggcatgaacc actgcgcctg gctgcttgta ccttttttgt 208980
gtgtatgtct tgttttgttt ttttgttttt gagacggagt tttgctcttg ttgcccaggc 209040
cagagtgcaa ttgcctgatc ttggctcacc acaatctccg cctcccgggt tctagcgatt 209100
ctcttgcctc atcctcccga gtagctggga ttacaggcat gcaccaccac acctggctaa 209160
tattgtattt ttagtagaga aggggtttct ccatgttggt caggctaatc ttgaactccc 209220
gacctcaggt gatccgcccg cctcggcctc ccaaagtgct gggattacaa gcgtgagcca 209280
ccgcgctcgg cctgtaactg ttttttaata gatctacagc tccttccctt aaggtctaaa 209340
gattctccat ccctgctttc aacagttaac aaagttccaa ctcagattct cataaattcc 209400
tccctgtctt cctctgggca attttattcc caaattcttg ccaccttttg ctttattcct 209460
tactattaga gaactataaa tatgtttctt tcagttttca tccttttctt ctatcttata 209520
aagttaggaa agggggaagg ataagagaac ctggtgacta cttaatccct ggtcagaaat 209580
cgttttatta ttattatctg tggcattttg aattaggctt agtgattcag ctatggcaag 209640
gaagtcccta cagtaccaga aagggtttgg gatggggttt tagaccttca gctgaagtcc 209700
agaaatgatc ttttccctag tagcagtgtg atgtggggat tttctctgcg tttaaaactt 209760
taaaagttgg tttacaattt gtctcagcat tgatgactga gtgtacaatg aatgtcacca 209820
gtccattaat gttctgtatg ggtaagtagg attccaatga aatagaactt ccaaatgagg 209880
aatatgaaat aggttttaca aataaaataa aatacaattt taaaaaacaa gtaaaagtgt 209940
ttttaaggtg gcccatatac cagtttctct gcttaaaaca gaattggctt ttctgcatga 210000
cagcaaatct ttgtttcctt agagcagggt ttcttgacag cagtgctatt ggcattttaa 210060
actggataat tctttgttgt gatgggcttt cctgtggact gtactatgtt ggtacacaag 210120
aaaaacagtg tactatgtga atactcactc aaagccagta gcactccctg attgtaacac 210180
caaaaaagtc tctcagcatt gccaaatgtc ccctgtggca gcagaatcac tccctgatga 210240
gaaccactac cctggagtaa aatctataac tatgtcttag aaaataacac agaaaattaa 210300
tatttctttc actctactcc ttccattagt gatcaaataa agaaggcatt tggcgctact 210360
tgccaaattg ttggctcaaa cttgtgctga accttttttg gttttctaca cttaagtttt 210420
tttgcctata acccagagaa ctttgaaaat agagtgtagt taatgtgtat ctaatgttac 210480
tttgtattga cttaattttc ccgccttaaa tccacagcat aaaaaatcac atggaaaaga 210540
caaagaaaac agaggcatta acacactgga gaggtctaaa gtggaagaaa ctacagagca 210600
cttggttaca aagagcagat tacctctgcg agcccagatc aacctttaat tcacttgggg 210660
gttggcaatt ttatttttaa agaaaactta aaaataaaac ctgaaacccc agaacttgag 210720
ccttgtgtat agattttaaa agaatatata tatcagccgg gcgcggtggc tcatgcctgt 210780
aatcccagca ctttgggagg ctgaggcggg tggattgctt gagcccagga gtttgagacc 210840
agcctggcca acgtggcaaa acctcgtctc tgttaaaaat tagccgggcg tggtggcaca 210900
ctcctgtaat cccagctact ggggaggctg aggcacgaga atcacttgaa cccaggaagc 210960
ggggttgcag tgagccaaag gtacaccact acactccagc ctgggcaaca gagcaagact 211020
cggtctcaaa aacaaaattt aaaaaagata taaggcagta ctgtaaattc agttgaattt 211080
tgatatctac ccatttttct gtcatcccta tagttcactt tgtattaaat tgggtttcat 211140
ttgggatttg caatgtaaat acgtatttct agttttcata taaagtagtt cttttataac 211200
aaatgaaaag tatttttctt gtatattatt aagtaatgaa tatataagaa ctgtactctt 211260
ctcagcttga gcttacatag gtaaatatca ccaacatctg tccttagaaa ggaccatctc 211320
atgttttttt tcttgctatg acttgtgtat tttcttgcat cctccctaga cttccctatt 211380
tcgctttctc ctcggctcac tttctccctt tttatttttc accaaaccat ttgtagagct 211440
acaaaaggta tcctttctta ttttcagtag tcagaatttt atctagaaat cttttaacac 211500
ctttttagtg gttatttcta aaatcactgt caacaataaa tctaacccta gttgtatccc 211560
tcctttcagt atttttcact tgttgcccca aatgtgaaag catttcattc ctttaagagg 211620
cctaactcat tcaccctgac agagttcaca aaaagcccac ttaagagtat acattgctat 211680
tatgggagac cacccagaca tctgactaat ggctctgtgc ccacactcca agacctgtgc 211740
cttttagaga agctcacaat gatttaagga ctgtttgaaa cttccaatta tgtctataat 211800
ttatattctt ttgtttacat gatgaaactt tttgttgttg cttgtttgta tataatacaa 211860
tgtgtacatg tatctttttc tcgattcaaa tcttaaccct taggactctg gtatttttga 211920
tctggcaacc atatttctgg aagttgagat gtttcagctt gaagaaccaa aacagaagga 211980
atatgtacaa agaataaatt ttctgctcac gatgagttta gtgtgtaaag tttagagaca 212040
tctgactttg atagctaaat taaaccaaac cctattgaag aattgaatat atgctacttc 212100
aagaaactaa attgatctcg tagaattatc ttaataaaat aatggctata atttctctgc 212160
aaaatcagat gtcagcataa gcgatggata atacctaata aactgccctc agtaaatcca 212220
tggttaataa atgtggtttc tacattaacc tgcttgctcc ataatttctt atctggcaat 212280
gggtatattt agagggttag gcaagctgca ttcacattcc caaaataaac tatgcaggta 212340
ccaccaaaga agaaggagcc tagtaaactt gcaggctttt agttgcactc caaggggcta 212400
tcctaggtgc aaggttagtg agaagtaggc cctcacacag cctgaagtcc agctttaagt 212460
catctcattc catgactgtt taagttatct gagaatgctg atgcaactaa tttcactgcc 212520
tcacagaagc aaaaataaat ctccagataa ttttaacatc cagaaccata aattagctct 212580
accaattttc atacacaatg tcgggccctc agaatgtaac caagagtagc atcagcaaaa 212640
atggccaagt aggtagcttc aaggaccttt tcctccacag aaacattgaa aaatcaagca 212700
aaaacattca gaaacaactt tgtcagaacc caggaaaata gtttacagca ccaagcattt 212760
gctgaatcaa gaaaaaggca acctagaaag aaggaaagct tggcagtgtt ttacttgccc 212820
tttccccatt ctcagctctg caacagtctt gaagacacag aacacattct cagcgtggag 212880
tcctggtctt gggtttctag ggagaagaga gatttttttt tttttttttg gagacagtct 212940
caccctgcca cccaggccag agtgcattgg catgatctcg gctcactgca acctcccggg 213000
ttcaagcaat ttgtgcctca gcctcccaag tagctgggac cacaggcaca caccacgaca 213060
cctgctaatt tttgtatttt tagtagagac ggggtttcac catgttggcc aggctggtct 213120
caaactcctg atctcaggtg atccaccctt ttcggcctcc caaagtgctt ggattatagg 213180
catgagccac cacatccagc cccagagatt ttattcttga aagattatgt ttgttctgac 213240
ctgtctggca gcctcaagga ctgacaaaag gcacctgcat ttgtttcgtg taactcagaa 213300
ctcagtcatg gtgtgaaatt ggcatgtttg tctctaaata ttgtaaggca aatgaacaac 213360
ctgctgccgc ctgaggcaaa aagttaccgt tgaggtaaac aaaggagtgc ctaaagcctg 213420
agaggaaagc agcagaattt cttagggaaa ttaggttatt caaaagcacc catgtatatt 213480
gggggattta gaaagccatg cacagtccca ggtcagtctg cacattcaaa cctgagaaga 213540
ccccaagatt tcatgtctgg ctgatgtgta ggctcagtgc aagcaggaag taaaggctaa 213600
ggccaatttc agatgggccg gataggcact gaaggtgtgt cccaacacag ccaatcagca 213660
aaaactgagg gtttttttct tcccttgtca atttttgggg caaccagttt tcaaagaaat 213720
ctcatcacac tggctgaacg ctagcaaaag gaagacacct tacagaccgt gtaagtcaaa 213780
gaacagaaac tttacaaaaa caggtcagaa aagtcactag gaccgggtgc tgtggctcat 213840
gcctgtaatt tcagtatttt gggaggccaa ggtgggagga tcacttgaac ccaggagttc 213900
gagacctgcc cgggcaacat agtgagaact cgtctctata aaaaaaaaat tttttttaat 213960
tagttgggca tggtggcaca ggcctatagt cccaaccact tgggaggctg aggtggaagg 214020
attgattgag cccaggggtt tgaggctaca gtcagctatg attgcgccac tgcattccag 214080
cctcgggaac agagtgagac tttgtctcta aatatataca agtcactaaa caaaccacta 214140
caacccacag caagtaacaa aaaccctgag gagtgaagaa tctggtttcc agagttaata 214200
gtaaagtaat attcagcttt caacaaaaaa ctgtgaagca tgcaaagaaa gtatagccca 214260
ctcagaaaaa attagaataa actatctgag gaagcataga cattggactt taagacaaaa 214320
tttgtaaatc aactgtctca aaagtgctca aagagctaag ggaatacagg gaaacaatgt 214380
ctcatagaga ttgtcaaaga aattataaaa aggaaccata aaaattagct gaaaagtgca 214440
ataaaaaaaa gggggaaaaa atggttctaa tagcagatat ggccagacag tagaaagaat 214500
cagtgaagct gaaggtagat caattgaaat tatccatctg aggagcagaa gaacaaagga 214560
aagaagcaat ttaagaaagc tatggattac caccaagcat accaaccttt gcaaaatagg 214620
agtttcagaa ggagaggaga gagaaagggg cagaaagaat atttgaagaa ataatggccc 214680
caaacacctc aaatttgtga aaacacttga atgtacccat ccaagaagtt tgcagaactc 214740
caaataggat aagtcaggaa agatccatgt cgagacatat ataaactgtc caaaactgaa 214800
aagaggatct tggaagcaga aagacagtgg caatcagaga aatgcaaatc caaatcacag 214860
tgagatacca cttcacatcc agaaggatgg ctataacaaa aaatacagaa aaaaaaaagt 214920
ggtgttgggg atgctggaaa atgggagcct tcttgttttc tgagatggag tgtcgttctg 214980
tcacccaggc tggagtgcag tggtgctatc tcagctcact gcaacctcca ccttttgggt 215040
tccagtgatt ctcctgcctc agcctctgga gtagctggga ttacaggtgc ttaccatcac 215100
acccagctaa ttttttttct ggctaatttt tatattttta gtagaaatgg ggtttcgcca 215160
tgttgcccag gttagtcttg agctcctgat ctcaagtgat ccacctgtct tggcctccca 215220
aagtactaag atttcaggcg tgagccaccc cgcccagcct gaaaatggga accttgtaca 215280
ttgctcatgg gaatgtaaaa tggttcagcc actgtagaca aagagtttgg tggttcctga 215340
aaagataaac agaattacca tatagcccag caattccatt cctagctgtc ttcccaagag 215400
aaaagaaaac atgcatccac aaaaatttat acacagatgc tatagcagca ctattcacaa 215460
tgacccaatg gtgtaaacaa cccatcaata tatgaatgga taaacaaaat gattatatac 215520
gtatgataca ttactcagcc ataaagatga agtacagata caggctacca tatggataaa 215580
cctcaaaaac attatgctaa gtgaaagaag acacaaaagt tcacatattc tatgattcca 215640
gttttatgaa atatccagaa taggtaaatt catagagtca gcagactggt ggtggttaaa 215700
tctggtgtga ggggaatgca gagtgactgc ttaatgggta tgggattttc ttctggggtg 215760
ttggaaatat gttggaacta gcctgtaatc ccagcacttt gggaggccaa ggagggcgga 215820
ttacctgagg tcaacagttt gagaccagct tgagcaacat tgtgaaaccc cgtctctact 215880
aaaaatacaa aaaatagcca ggtgtggtgt cgggtgcctg taatcccagc tactcgggag 215940
gctgaggcag gagaatcgct tgaacttggg aggcagaggt cacagtgagc cgagattgca 216000
ccactgcact tcagcctgag tgacagagtg atactctttt tcttgtttgt ttttgagaca 216060
gagttttgtt cttgttgccc aggctggagt gcaatggcgc aatctcggct cactgcaacc 216120
tccgcctccc aggttcaagc gattctcctg cctcagcctc ccgagtagct gggattacag 216180
gcgcctgcca atatgctcag ctgatttttg tatttttagt agagacaggg tttctccatg 216240
ttggtcaggc tggtctcgaa ctcccgacct cagatgattc acccacctcg gcctcccaaa 216300
atgctgggat tacaggcagg agccactgca cctggccagg gttcttacac tatatatgaa 216360
tcagtataat aatttttgaa agtagaccat ggttaattca tgatgtatat tgtaagcctt 216420
tgggcaataa ctaggcaaaa attttaatgg atataaaaca gattcataaa agtactcagt 216480
taattttaga attacagaaa atgaggaaaa ggggacaaag aacaaagaaa ataaacagaa 216540
aagaagtagc aaatggatat atttaagccc aataacatta aatgtaagcg gtttaaataa 216600
actaaaagat agatcatcag attggataaa aagtaattat aatctatcta caagaagccc 216660
acttcaaata gaaaagatat atacagataa acaagaaaag gatgggaaag atacacaaac 216720
cctcatcaaa agaaagctag agtgtctata ttactaccaa agttgacttt agaatgagga 216780
atgtcaccaa ggataaaaag gaacattaca taatgataaa gatgttaatt cacaaagagg 216840
tataacaatt ctacatatgt acgtacctaa caacagaggt gctagaaact gacaggactg 216900
aaaggagaaa taaacaaatc cacagttata gctagagact tgagcatttt aagtaacaga 216960
actagaaaga aaatcaataa agatattgaa gacctgacaa ctctgtcaac caccttgacc 217020
taatgaatat ttatagaaca atatacccaa caatagggga atatattatt tgtgaagtgc 217080
ttgaaacatt caaacaatat gaactatatt ctgggtcatt aaaaaacaac tccacaagtt 217140
ttaaagaatt gaaatcatac aagtatgaag tcaatacata caacttgaag tcaacaatac 217200
acacaacttg aagtcaatac aagtcataca acttgaagtc aataatacat agttggggat 217260
tttccaacta tgtattattg acttcaagtt taactcaatt ttaagtcaag tctaattccc 217320
caaatatgtc caaatatttg gaaattaaac aacacacttc tacacttgat cttagccaaa 217380
aggccgagaa acgataaaca acacacttct aaacctactc atcaaagagg aaatcacatg 217440
taaaactaga aaaatttaaa cggtggcttg tgcctataat ctcagctact ctggaggctg 217500
aggcaggagg atcacttgag gtcaggaatg tgagagcagc ctgggcaaca tagcaagacc 217560
ccatctctag aaaacaaaaa aattaaaaat ttggctgggt gcagtggctc acgcctttca 217620
tccaacactt tgggagtcaa aggcaggcag atcacttgag catgggagtt caagaccagc 217680
ctaggcaacc tggtgggacc cccatctcta ccaaaaatag aaaaaattat ctgggggtgg 217740
tggagcatgt ctctattccc agcttcttgg ggaggctgag gtgggaggat tgcttgagcc 217800
caggaggcag aggtggcagt gagccgagat tgtgccaccg catcttggcc tgagtgatgg 217860
agtgagatcc catttcaaaa aaaaaaaatt agctgagtat ggtgacacac acctgtagtc 217920
ccagctgagg ctaaggcagg aggatcactt gatacacata tgcatacata catacatata 217980
tgtatgtagg tgtagttata taatatatat aggtatgtac aactataaat ttcttcctca 218040
gcactgcttt agttacatcc cataaatttt ggtgtgttgt atatttgttt cattcctctc 218100
atagtatttt ataatatccc ttgtgacttt ttctttaacc aacgggttat ttaggagtgt 218160
gttgtctaat ttcctcatat ctgtgaactt ctcaaatgtc cttctgttgt ttatttctaa 218220
ttttgttcca ttatagttgg agaaaaatac ttggtattac tgcagtcaat ttaaatgtat 218280
tgagatttgt ctcatatgtc ataggcctaa catatggtct atcctggaaa tgttccattc 218340
ccacttgaga aaaatgtatc ttatactgtt gtttggtaga atgttctata aaaatctgtt 218400
aggtctaatt ggattatagt attgttcaag tcttttatta ccctcttgat cttccgccta 218460
tttgttttat tagtaaaagt ggcatattta agtctccaac tgttattttt taattgtctg 218520
tttctctctt caattctgtt atttttttgt ttcatgtatt ttggggttct gttgttaatt 218580
acatatgttt gtagttatgc cttgctaata aattgatatt ttatcattaa aatatatccc 218640
tttttataac tggtaacaat ttttgcctta aaatctattt tgtgggatac tagaatagcc 218700
actgcagttc tctctgagtt actgtatgca tggtatagct tttgccatct tttttttttt 218760
ttttgagatg gagtctcact tgtcacccag gctggagggc aatggcatgg tctcggctca 218820
ttgcaacctc cgcctccaag gttcaagcaa ttctcctgcc tcagccacct gagtagctgg 218880
gactacaggc tcatgccacc atacctggct gatttttgta tttttagtag agacagggtt 218940
cactatgttg gccaagctgg tctcgaactc ctgacctcgt gatccacctg ccttggcctc 219000
tcaaagtgct gggattacag gcgtaagcca ccacgcctgg ctgccaacct tttactttca 219060
acatattcat gtctttgaat ctagtgtgtc acttgtaaga caacatatag ttgaatcatt 219120
ttttaaatcc attctgccat tctcttactt ttgactaggg tgtttaatca atttcaagtc 219180
attactatta aggaaggaat tatgtctgcc attttgctat gttctctata tgtcttatgt 219240
catttttcct ttctattttc cattattgtc ctcttttgta ttaaatagat attttctaat 219300
ataccatttt aattcctttg tcattttttt ttttttacta tgtatatttt tgagtcattt 219360
cccttgttgt tttggagatt acaattaaca acttaattta acaatctagt ttaggttact 219420
accaacttaa tatcaatagt atacaaaatc tagctcctat gtagctctgt tccctttccc 219480
cttctttgca atgttgtcat aattacatct tcatacattg tgtgcccatc agcacaggtt 219540
tataattata actttatgca gtagtcttta aaatcattta aaagaaaaaa agaattataa 219600
gcaaaaatat attcatatgg tcttttatat ttatctattt agttaccttt accagtgctc 219660
tttatttctt catgtggtct caaattactg tctcgtgttc cttcatttca agatggactc 219720
cctttagtat ttcttgtgtg acaggtttgc taatgacaaa ttgtctcagt tttgtttatc 219780
tgggaatgac ttacattttg cttcatcttt gaaggttggt tttgttggac ataaaattct 219840
tggttgacag tcttttttcc agcactttga atgtcatcct atggctttct ggcctccatg 219900
gttactgata agaagtcagc tgttaatcat gtgaaggagc tcttctaaat gattaattgc 219960
atttctcttt cttcaagact cttttctttg gattttgaca gtttgattat gatgatgtgt 220020
ataggtgtgg atctttttaa gtttgtccta gttggagttc attgagcttc ttggatatgg 220080
agataaatgt ttttcagcaa atttattttg tttttcagca aattgtttct tcaaatatta 220140
tttctgtcct tttctcttct actaggactc tcattatgtg tatgttggta tacttgataa 220200
tgtcccacag gtctctgatg ttctattcat ttttatttat ccttcttcct ttctgttcct 220260
cagactagac aatcacaatt gacttttctt caatttttct tcaactgact tttttaagaa 220320
cttcaagatg cctcctgcat gaacttgtag gttttgtttg tgagttattg ttgactctaa 220380
aggaacacct cgttgcagaa acaggaacca ggtctaggag ttttactgtc aagccatata 220440
aatatgcctg acagtaggcc ttgagccatt taatatattc ttcactgata attctggtgt 220500
ttcctagaga tccaatggat caaaaaagac ttttgctgag tcttggtttt tacttaatca 220560
catttgcttg tgagtccctc tagtgaattt ttaatttcag ctattgcact ttttaacatt 220620
gttaaaaaca tgatttcctt taatgctttg aacacattta aaatatcttc aagtatagtt 220680
tctatagatt gctattttcc tgtgtaagaa caatacttta tttctttgct tgtctcataa 220740
ttttatacag aaacctgaca tttctttttt gttgttgttt tttgagatag agtctcactg 220800
ttgcctaggc tggagtgcat tgttgctatc tcagctcact gcaacctctg cctcccggac 220860
ttaagcagtt ctcatgcctc agtttcctga gtagctgaga ctaaaggcat gtgccaccac 220920
gcctggctaa tttttgtatt tttagtagag atgaggtttc accatgttgg ccaggctggt 220980
ctcaacctcc tgacctcaaa tgacccacct gcctcagcct ctcaaagtgc tgggattaca 221040
ggcatgaact accataccag gccaaaaact gacatttcta ataatacaat gtgactactc 221100
tggaaatcaa agtatttccc cttctaagga cttgttgatg ttgttttttt gttgttgttg 221160
atgttgggtt tttttttagt ggcttttcca aatgaatctg tgaagtatgt cttctttgtc 221220
actgaattct ctgctcactt agcttagtgg tcagctagtg attagatagt tacttaaatg 221280
actgaatcta tttcccctcc cagccatttc caagaggctc tgtgtatgtt tgtgttgggg 221340
cacccttcac actcaggcag gcatttaaaa atgctgactt agctttcatc atttttttgc 221400
agtgcctcaa ggtcagccag atgttgtgtt agaacttagg accttcttag gtctttcctg 221460
gcatgtggac agatttgagc atgcacataa gcccatgtat gtgcatggcc ttctagagtc 221520
ccatgaatat gtcagcagtt tttttgtttt tttgagatgg agcttagctt ttgttgccct 221580
gtctggagtg caatggcgca atctcggctc actgcaacct ctgcctcctg ggttcaagca 221640
attctcctgc ctcagcatcc cgagtagctg ggattacagg cgcccgccac catgcctgga 221700
taatttttgt atttttagta gagatagggt ttcaccatgt tggtcaggct agtctcgaac 221760
ttccgacctc aggtgatccg ccagccctgg cctcccaaag tgctggatta caggcataag 221820
ccactgcacc cagcccctaa tttttgtatt tttagtggag atggggtttc accatgttgg 221880
ccaggctggt ctcgaactcc tgacctcagg tgatctgcct gtctcagcct cccaaagtgc 221940
tgggattaca gttgtgcgcc actgcaccca gtcatgtcag cagtcttgaa agcctcctat 222000
ggacacctca ttccccagct cttctttcga tggttttttg tttgtttgtt tgttttggct 222060
ggggggcagc ttgtttgcct caaatgttac cactacctta gttagctgca tgttagccat 222120
gctactgatt attttcaaca aatgtcccag gtgaaaggct attctcaccg agtggggtct 222180
gagttgggtc aaataaaggc aaacatttgt ttgttttttt ttttcgagac agggtcttgc 222240
tctatcaccc aggttggagt gcggtggtgt gaccatagct cactgaagcc tcgaactcct 222300
gggctcaagc aattctcctg cttcagcctc ctgagtcgct gagactacag gtgtgtgcca 222360
ccacacctaa ttttttttta tttgttgtag gggcagtgtc tcactctgtt gcccaggctg 222420
gtcttgaacg cctagcctca cacaatcctt ccacctcagc ctcccaaagc aatgggatta 222480
taggcctgag ccaccgtggc tggccaaggc aagtcttgtg aatgagggtt tccaggcaat 222540
tgtcagacag gccaaataag gacagttctt aaataatgct attttgggga tctccaaacc 222600
cattcggtcc tagcaagtgg ctgctaggct actggtcttc actatgattg tagaactgtt 222660
ggttttgaag actaatgagg agttgaggag agtgttgaga ataaggcaag ttaaaacacc 222720
acaaagctca ccattcttac tgagatacag ccttaaaaaa aaaagttcct tgggttgttg 222780
caaagctttg gttaatttca gaattctgaa catttttatt ttacaagtta tctcaatatt 222840
ctcattgctt ttatggagga ataggttttt agaggccctt atgccaccac tcctactgac 222900
ctcacctcat tcagttattt gaaggcatca tgccattgtc tgcttcaatt ttttctgttg 222960
caaaatcata gtcagtttaa ttattgttcc tatgaaaata atctttttct ccttggttac 223020
tttttttttt ctttgagaca gggtctggct ctgtcactca gtctggaatg caatggcacg 223080
atttcagctc actccaacct ccatctccca ggctcaagcc atcctcctac ctcagcctcc 223140
tgagtagcta agaatatagg cacgcaccat catgcccagt tagtttttgt atttttagta 223200
gagacagggt tttgctatat tgcccaggct ggtcttgaac tcctgggctc aaagcaatct 223260
acctgcctct gctctttggt tagttttaag atatttctgt atctttggtt tttagcagtt 223320
tttctctgat acacctcagt gtgactttgt atgtatcctg cttagggttt atacagtgct 223380
atagacctaa ttgtgtctcc ccacccccca atccacatat tgaagcccta actctcaatg 223440
tgaatgtatt tggaaatagg ccctataagg agtgaattaa ggtttaatga ggtcttgagg 223500
gtggggttct gatactacag aatgtgtgct tgtaaagaaa taccagagag cgccctttac 223560
cccgcatccc actctgccat gtaagaacac agcgaaaaag caccatctgc aacccaggaa 223620
gtgagctctc accagaaccc aactgtgctg gcaccctgat cccagacttc cagcctccac 223680
aatttgaaaa catttctgtt caagccaccc attctggctg ggcatggtgg ctcacacctg 223740
taatcccaga actttgggag gccaagccag aaggatcacc tgaggtcagg agttcgagac 223800
cagtctggct aacatggtga aactacgtct ctactaaaaa tacaaaaatt agccaggcat 223860
gatagcacgc atctgtagtc tcagctacta gggaggctga ggcacgagaa tggcttgaac 223920
ctgggaggcg gaggttgcgg tgagccgagg ttgcgccact ctgctccaga ctgagcacaa 223980
ctccatctaa aaaaaaagcc acctattcta tggtatttta tggcagcatg agctgactaa 224040
tacatagagc ttcagtccca ttcttttttt ctgagatgcc agttacatgt atgttagact 224100
tctttaccat aacctcttta tgtttctcat ccttttgcct acccatgcct tagtaacaga 224160
tgacccttaa ataatgcagg ggttagggtg ctgtcccacc ccatgcagtc gaaaatttgt 224220
atataacttt tttttttttt taagatggag tcttgctctg ttgccaggct ggagcgcagt 224280
gggatgatct tggctcactg caacctccgc ctcctgggtt cagggttcaa gcgattctcc 224340
tgcctcagcc tcccgagtag ctgggattac aggcgtgagc taccgtgccc agccgtgtat 224400
aacttttgac tccccccaga gctcagctat gaatagctta ctattgactg gaagccttac 224460
tgataacata aacagtccat taacacatat tttgtatatg tattatatac tgtattctta 224520
caataagtaa gctacaggga ataagatgtt atttaaaaag tcataaggaa aatatattta 224580
ctattaagtg gatgtggatc atcataaagg tatttgtctt tatggtcttc atgctgagga 224640
ggaggaaaag gaggaagagg aagaagaggg ggggaggagg aggaagggtt ggtcttgctg 224700
tctcaggagc agcagaggtg gaagaaaatc tataagtgga tccacacggt tcaagcccat 224760
gtttttcaag ggtcaactgt aactattttc ttacaactat cacccagtct ttttctcttt 224820
ttaactgact agccagtaag taaatccatc tcgtttgtaa ttttttgcat ttttcaattc 224880
tagaatttcc atccgattac tttttatagt ttccagccaa agtactccat cttgtcttta 224940
aagtttttga aaattcaatt tttaaaaatc ttgcttgggc tgggcctggt ggctgtggcc 225000
tataatccct gcactttggg aggctgaggt ggaaggactg cttgaggcca ggagttcagg 225060
accagcctgg gcaacatagg gagaccccgt ctctacaaaa cattaaataa ttacaccagt 225120
atggtggcat gcacctgtag tcctagctac ttgggaggct gaggtgggag gatcacctga 225180
gcccaggagt tgaagctgca gtgagatata atcacactac tgcactccag cctagataca 225240
gagcaagacc ctgtctcaaa atcttgtttt gcaactccat tagctgtatg gtgctttttt 225300
ttttcacagt tataataact ttaatttatc ttgcatttta cagaaatcta tgaacaattt 225360
ttaaaaagca cctccttacc tcatatcatg tttctctgac aggtgttaaa gtaggcaatg 225420
agtatgtcaa cagcttgagc atcagcgtct tgtaaggact tcagaccaat caaccactcg 225480
ccaaaaatct tggcaggttt tttatcttgt ttttaataca acggtatatc cactctgatg 225540
gcaaacctgt ccagccacat ctccacaaca cgctttgcaa aatcagtgat tagcaaatta 225600
gttagctttg gcacggtggt gtgctcactt gcccattaca gcctggtagc tggttttgat 225660
gctggcaaca gaacatctag aatgacaagt ttcgcactgt aggaaataga ttcgcatgtc 225720
cttctgcagg attgtgtcca gtaatcggca cgtgtgacaa gtgacatatt ccttgatata 225780
tcttctcaag acattttcta tctgtttctg ttggaatctt cctttgatta caagttggtt 225840
attaccatct atagaaccac ttgtacccaa ttcagccaac aaaaatgcac ggagatgttt 225900
gggctgatga tgtaatagtt tacagatatg tgtaaagttg acaaaagacg ttttcttggt 225960
tcctactcgg acgacctgtg gaggtttcat gacaaatttc cttatgtccc cagcaaccat 226020
acctggattc ttttccctca tgatgttgaa cactcgattc agtagctcct catatgtgta 226080
gtctctttct gagcctgccc aagcagggcc tgtctgatta ctgaatgaga taccatcatc 226140
ttttttgctg tcttcctctt ctagaacttc atctttctct agtatttcat cctcatctgg 226200
gaacttgacc ttcttcttct tctttttatt gccaagcata atgtcaaggt cattctctgg 226260
ttcagttggt tcttgaacat cactttcaat cttaagatcc tttacacctt cttcagcttc 226320
atcaatatca aatatctttt tagttttttt tttctttttc ttttgattaa agaagttcaa 226380
gtcatctaga tcaccagcag catctttttt cctagtgtcc tcttcatcag cttccaaatc 226440
tttgtcctca gttggctctg gccccacttc ttttgtttct gaaggctggg ttatctctgt 226500
ttgggtatcc gcttcctcat ctaacataaa aggcttcttt ttcttctttt tctttttgct 226560
cacagtagga tcaaaaatca tctcgtcccc agacatggct gcggcttgag tgggcttggc 226620
acggacggga agtcagacgg gtcaacccca gaccccggct gcagcgctgc tcctgccggt 226680
acctctccca ccaccgcact ggtgctttgt tgttgttgtt gtttttcttt ctttctattg 226740
tctgtctttt ctcttggttt tggatcaagg gctatctcct tgtatatcag ggtttttttt 226800
tttgtttttt ttttttttta tatggagtct tgctctgtcg cccaggctgg agtgcaatgg 226860
caggatctcg gctcactgca acctctgcct cctgggttca agcgattctc ctgcctcagc 226920
ctccaaagta gctaggacta gaggcgtcta ctaccacacc cagctaattt tttgtatttt 226980
tagtagagac agtgtttcgc cgtgttagcc aggatggtct cgatttcctg acctcgtgat 227040
ccgcccgcct cggcctccca aagtggtggg attacaggcg tgagccattg cgcccggccc 227100
gtataccagg ttcttttttt aaggagtgta aacagctata ttttatgggt gcacaatatt 227160
tttaaaattg taacatttaa gaactttttc atatctgcta attaattcta cgaacaatat 227220
tgatgcctct ttaatatatg ataggacact atttacttat tttctttttt aaaaaatttt 227280
aattaaaaaa aaaaagacta ttggctgggc gcagtggctc atgcctgtaa tcccagcgct 227340
ttgggaggcc aaggcgggca atcacctgag gtcgagagtt tgagaccagc ctggccaacg 227400
tggtgaaacc ccatctctac taaaaataca aaaatttagc caggcatggt ggcaggcact 227460
tgtaatccca gctactcggg aggctgaggc aggagaatcg cttgaacctg ggtagcggag 227520
gttgcagtga gctgagattg ccctattaca cttcagcctg ggcaacaaga gcgaaacttc 227580
gtctcaaaaa aaagaagaac ctagtaacag tacactttaa attcttctct ttaatctttt 227640
ctatgattgt ttttatttat aaatgtgcaa taaacccata atacattcca ctatatttct 227700
ttttgaaagt caataatttt tatagagatc aaaaataaaa gtaaaaaagt ttttatattt 227760
aattttagtc atttccagaa atcttcattt tttgtgtgta gatttctttg ctttgtgtat 227820
aataccagat tctgtgatat catattcctc ttgcttaaag aacttttttt tttttttttt 227880
ttgagacaag ctcttgcttt gtcacccagg ctagagcata gtggcatgat cacagctcac 227940
tgcagcctct acctcttggg ctcaagcagt cctcccaccc cagcctccca aggagctggg 228000
actacaggtg tgcaccacca tgcctggcta attcttttct ttttcctgta gagacaaggt 228060
ctcactatat tgcccaggct ggtctcaaac tcctgggctc aagtgatcca ccttggcctc 228120
ccaaagtgct gggattacag acgtgaacga ccgcgcctgg cctaagaact ctctttaaca 228180
tttcttttct ttctttcttt cttttttttt ttttgagatg gagtttcact cttgttgccc 228240
aggctggagt gcagtagtgt gatttcggct caccacaacc tctgcctccc gggttcaagc 228300
gattctcctg cctcagcctc ccgagtagct agggttatag gcatgtacca ccacgcctgg 228360
ctaatttttg tatttttagt agagatgggg cttctccatg ttggtcaggc tggtcctgaa 228420
ctcccgacct caggtgatct gctcacctcg gcctcccgaa gtgctgggat tataggcggg 228480
agccaccgtg cctggccaat attgcttata gtacaggact gttggtaata aattgttagg 228540
tttgttttct aaagaagtat tcattttgcc ttcatttttg aaaagtattt tcattgagta 228600
tagaactctg gttaaaagtg ggttttttca atatttttta aatgtcactt tattgtcttc 228660
tggtttgcac agtttctaat gaaaaacctg gtataattct tatcttcgtt cctctgcatt 228720
taatgtgtct tctgtttttg gtcgtcttca aaattttctc tttctctttg gttttcagta 228780
gtgtggtggg cggctaggtt gctagagggt gtttcttttc ctttgagaca agcatcttgc 228840
tctgttgtcc aggctggagt acagtggtat gattacagct cactgcagcc tcgaactcct 228900
gggcaggagt cagtacagag cagcaaatgt tgaggtttca caggggcctc tctggaaacc 228960
tcgccctcaa ggccctggct tgtcttaaag agctctgaaa tgcctctggg atcatccttc 229020
cattgcctta ataaatggaa cctggctttc ctctattcct acaatctctt tagcaaattt 229080
ttgcttggct atggccttgg tttgctctcc taaagatgcc ttttcactct ttaccaggcc 229140
aggctgtgaa ttttctaaat ctttctgctc tgctttcctt ttagtgataa attcaatctt 229200
taaattattt ctcatcaggc ctggtgcggt ggctcacgcc tgtaatccca gcactttggg 229260
aggctgaggt aggtggatca cttgaggcca ggagttcgag accatcctgg ccaacatggt 229320
gaaaccctgt ctctactaaa aatacaaaaa tcagccagac gtggtgctgc acgccagtaa 229380
tcccagctac cagggaggct gaggcaggag aatcacttga acctgggagg tggagcatgc 229440
agtgaactga gatggcgcca tagcactcta gcctgagcga cagagccaga ctccatctca 229500
aaaaagaaaa agaaagaaat acaattaatt atttctcatc agttctttgg taggggtaaa 229560
agaattcttt ctatcctctc acatcttact acatgcagtt aaaagtagcc acgaagctcc 229620
ttcaatattt ttcatcttag agattttttc cactagatat tctatttatt ttttattatt 229680
gtttattttt ttgagatgga gtttcactct tgtcacccag gttggagtgc aatggtgcag 229740
tcttggctcg ctgcaagctc caccttctgg gttcaagcga ttttcctgcc tcagtctccc 229800
aattagctgg gattacaggc gtgcgccacc atgcccggct aatttttgta tttttagtag 229860
agacgaggtt tcaccatatt ggtaaggctg gtctcgaact tctgacctca ggtgattcac 229920
ccaccttggg ctcccaaagt gctgggatca caggcatgag ccaccgcgcc tggcctactg 229980
tccatatttc tatgaacatt ctaaacatca ctgtagtaat ctctaggaag gtccaaactt 230040
tgctgacagc ttttctcttc ttctgagcct tcttaagaat tgcccttagt gcttcattca 230100
tggcagtcta ggtgttttct agcccgctcc tctaaattct tccagcctct atgcattacc 230160
cagttccaaa gttgcttcca cattttcagg catttgttat agcatcagcc ctgcttcttg 230220
gtaccagttt tctgtcttgg tccattttgt gctgctataa gagaatatct gaagactagg 230280
taacttataa agaagaaaat gtatttagct cccaaacttt caagttctgg agcctggaaa 230340
gtccaatatg aaggtgccag catctaatga gggctttcct gccacatcat cccatggcgg 230400
aaaatgagag ggtgggaaag gatgagagaa ggctgaactc gattttataa caaacccact 230460
atcttgacga tgaacccact cccgtgataa caaccttaat ccattcatga aggcagatca 230520
ctcatgacct aatcacctct tacacatccc atctctcttt tttttttttt ttttttgtat 230580
ttttttttaa atttattttt ttattgataa ttcttgggtg tttctcacag agggggattt 230640
ggcagggtca tgggacaata gtggagggaa ggtcagcaga taaacaagtg aacaaaggtc 230700
tctggttttc ctaggcagag gaccctgcgg ccttccgcag tgtttgtgtc cctgattact 230760
tgagattagg gattggtgat gactcttaac gagcatgctg ccttcaagca tctgtttaac 230820
aaagcacatc ttgcaccgcc cttaatccat ttaaccctga gtggacacag cacatgtttc 230880
agagagcaca gggttggggg taaggtcaca gatcaacagg atcccaaggc agaggaattt 230940
ttcttagtgc agaacaaaat gaaaagtctc ccatgtctac ttctttctac acagacacgg 231000
caaccatccg atttctcaat cttttcccca cctttcccgc ctttctattc cacaaagccg 231060
ccattgtcat cctggcccgt tctcaatgag ctgttgggca cacctcccag acggggtggt 231120
ggccgggcag aggggctcct cacttcccag taggggcggc cgggcagagg cgcccctcac 231180
ctcccggacg gggcggctgg ccaggcaggg gggccgaccc cccccacctc cctcccggac 231240
ggggcggctg gccgggcggg gggctgaccc ccccacctcc ctcccggacg gggcggctgg 231300
ccgggcagag gggctcctca cttcccagta ggggcggccg ggcagaggcg cccctcacct 231360
cccggacagg gcggctggct gggcgggggg gctgaccccc cccccacctc cctcccggac 231420
ggggcggctg gccgggcggg gggccgaccc ccccacctcc ctcccggacg gggcggctgg 231480
ccgggcgggg ggctgacccc ccccacctcc ctcccggacg gggcggctgg ccgggcgggg 231540
ggccgacccc cccacctccc tcccagacgg ggcggctggc cgggcggggg gccgaccccc 231600
ccacctccct cccggacggg gcggctggcc gggcgggggg ccgacccccc cacctccctc 231660
ccggacgggg cagctggccg ggcagagggg ctcctcactt cccagtaggg gtggccgggc 231720
agaggcgccc ctcacctccc agacggggcg gctggccggg cggagggctg acccccccac 231780
ctccctcccg gacagggcgg ctggccgggc ggggggctga ccccccacct ccctcccgga 231840
cggggcggct ggccgggcag aggggctcct cacttcccag taggggcggc tgggcagagg 231900
cgcccctcac ctcccagacg gggcggctgg ccgggcggag ggctgacccc cccacctccc 231960
tcccggacgg ggcggctggc caggcggggg gctgaccccc ctacctccct cccggacggg 232020
gcggctggcc gggtgggggg gctgaccccc ccatctccct cccagacggg gtggctggcc 232080
gggctgaggg gctcctcact tcccagtagg ggcggccggg cagaggcgcc cctcacctcc 232140
cggacggggc ggctggccgg gcggggggct gaccccccca cctccctccc agacggcacg 232200
gctggccagg cggggggctg acccccccac ctccctcccg gacggcacgg ctggccgggc 232260
gggggggctg accccccacc tccctcccgg atggggcggc tggccgggcg gggggctgac 232320
cccccccacc tccctcccgg acggggtggc tgcccggcgg agacgctcct cacttcccag 232380
atggggtggc tgccgggcgg agaggctcct cacttctcag acggggcggt tgccaggcag 232440
agggtctcct cacttctcag acggggcggc cgggcagaga cgctcctcac ctcccagacg 232500
gggtctcggc cgggcagagg cgctcctcac atcccagatg gggcggcggg gcagaggcgc 232560
tccccacatc tcagacgatg ggcggccggg cagagacgct cctcacttcc tagatgtgat 232620
ggcggctggg aagaggcgct cctcacttcc tagatgggat ggcggccggg cggagacgct 232680
cctcactttc cagactgggc agccaggcag aggggctcct cacatcccag acgatgggcg 232740
gccaggcaga gacactcctc acttcccaga cggggtggca gccgggcaga ggctgcaatc 232800
tcggcacttt gggaggccaa ggcaggcggc tgctccttgc cctcgggccc cgcggggccc 232860
gtccgctcct ccagccccac atcccatctc tcaacactgt tgcattaggg attacattta 232920
caacacaaaa ctttgtggga tacattcaac tcatagcatt tgcataattt atgaagttaa 232980
aaaatcaatc aaccaaaaaa ttgttccccc ttaaacatgg gagaaggaaa gatgggctga 233040
actaaggttt tcggtttttt tttgtttgtt tttgtttttg tagagatggg gtttcaccat 233100
gttgcccagg ctagtcttga acttctgagc tccaaagatc ctcctgcttc agcctcccaa 233160
attgctggga ttacaggcat cagccaccat gcctggcctg aactagtttt ttctacagct 233220
atttttctgc aaccaggcaa aatttattct actctcctca aacagaagga agtagagaac 233280
ttactgggtc aggcatagtg ctaaacattt taaaaccata tgatatgagt gtgattatta 233340
gccggccttt ttttaaaaag gctaaactca aaacaaaaca ataaaacaac acatgctgag 233400
aaagagtaaa tgccttgccc aacatcacag agttaggaag tttagagaaa agtccatact 233460
tttaactact gggtaacact accttctata ttcaggaata tatatataaa cactgtataa 233520
aaaattcaga tgctaaggaa tatatatata aacactgtac aaaaaattca gatactaagc 233580
aaaggtaaga agatgagaat attgtccatg tgatatttgg cgcattgcca cactagtgac 233640
tacagaatct agagtattag attcattgaa tttccagctc ttgaatcttc atgttatatg 233700
ttcattataa ttaaatccac tacaataaca acaacaaaaa tcagatagtc tggagcttct 233760
caggacattc atttaccaat accattataa caaagctctt tgagggcagg agccttgtcc 233820
tattaatatt tatattctcc actagatatt tatatctagt atgatagacc ctggataaat 233880
ttctaactga aaatctccct ataataacag atttccaaaa tccagacaag gcacatttat 233940
ttcaagtaat atttgctaga gaaatgctct agtttaccat aaaagaatta gaagagcccc 234000
ttgagctact tttctccatt atcattattg tagttgtcat tggtattatc attattatca 234060
ttaccatggc aaccaccatt tattaagtgc ttactctatg ccagtgattg acatgcaaca 234120
actcatttta tcatcacaat actattatga gaaagatatt accatcccta tgctacttaa 234180
aggacactgg ggctcgaaga agttaagaaa ctaatcaggc cgggcttggt ggctcacact 234240
tgtaatccta gcactttggg agggtgaggc gggcagatct caaggtcagg agttcaagac 234300
cagcctgacc aatatggtga aacctcgtct ctactaaaaa tacaaaaaat tggctgggcg 234360
tgttggcaca cgcctgtagt cccagctact cgggaggctg aggcaggaga atcgcttgaa 234420
cctgggaggc agaggtttca gtgagccaag atcacaccac tgcactccag cctgggcgac 234480
agagtgagac tctatctcaa aaaaaaaaaa aaaaaaagag gaaactaatc aaagcacaca 234540
gtaaaattgg tgggacttga attcaaaatc atgtttgcat ttgacccatt attgggtata 234600
tactcaaata aatagaactc attctaccat aaagacacat gcacttgcat gtttgttgca 234660
gcactattca ccatagcaaa gacaaggaat caatccaaat gtccatccta agtagactgg 234720
ataaagaaaa tgtggtacat atacaccatg gaatagtaca cagccataaa aaagaatgag 234780
attatgttct ttgcagcaac atggatggag atggaggcca ttaccctaag caaactaatg 234840
caggatcaga aaagcaaaca ccacatgttt tcacttatca gtaggagctg aacattgaga 234900
taaagaaggg agcaatagat gccagggcct aattgaaggt ggagggtagg aggagggtga 234960
ggattgaaaa actcccaaaa gagttctatg cttattactt gggtgacaaa ataatatgta 235020
caccaaaccc ctgcaacatg tgatttatct atagaaccaa cctgtacatg tacctctgaa 235080
actaaaagtt ttaaacaatc acgtttattt gactccattc attcagcttt tcaaaaatat 235140
ctattgcagc tactatatgc cagacacttt ttagaaaccg ggttcaaaat tcaaaaagta 235200
caaaaatggc agcataaagc cacttcccat ttcattctcc cctgcccctc tccaccacta 235260
tgtctacacc accaactagc ttcccccttt tgagcaacca atgttatcag tttctggtga 235320
ggcctgtaac ttcagtcagg gagttctttt ttgggtcaaa ccttggcgtg ggcaatctct 235380
cctaccactc tcacacctct ccgaatgacc tcagacttct tcaataagca tttttttttt 235440
ttttgagatg gagtctcgct ctatcaccca ggctggagtg cagtggcacg atctcggctc 235500
accacaacct ccacctcccg gcttcaagac attctcctgc ctcagcctcc caagtagctg 235560
ggattacagg tgcgtgccac catgcccagc taattttttt attttgagta gagatggggt 235620
tccaccatgt tggccaggct ggtcctgaac tcctgacctc gtgatccacc cacctcagcc 235680
tcccaaagtg ctgggattac aggcatgagc caccatgccc agccagcgat aagccaatct 235740
taaataattc agtgtttcag gcccatttca cgtctggggg aaacgtgtcc tcaggaagcc 235800
agcttatgtt tgaactgaga tgaagtcctt gtggaacggg agagtgagtt cctgtaggtt 235860
aattcacaat gtctttacca tgaagcttct gtttctctag gaggcggcca tcctcagctc 235920
tcatagatcc ccacacttag aaagtccaca ctacttccct tccacctctg tctgaaccaa 235980
gctctctctg acagccctgc agaacacagg tcttgcgact tcaaaatgtc gcatcacaac 236040
attttgttgc cttttccttc tctcaaaata gaatctttac agtgctcctt aaagtgaagg 236100
atggtggaat tttgtggcca tatgatctga gaggacgttg tgaaaatttt aaaaacaggg 236160
acagaatttg gactggcttt caggttttcc aatagacttt gagttctctg gcagtaagtc 236220
cctcttcccc tactttctcg gtgctgatga gaagggcacc tggttggtga aaaacatgca 236280
aattaagagg cacaggatca agaggaacag gctgctcctc ttgaatagag gtgtccgtgg 236340
tccagacagc atgagtcctg gtttatcatt gagattaata gtactggcca ggtgtggtgg 236400
ctcacacctc taatcccagc actttgggag gctgaagctg gcggatcacc tgaggtcggg 236460
agttcgagac cagcctgacc aatatggtga aaccgcatct ctactaaaaa tacaaaaact 236520
agccgggctg tggtggcacg tgcctgtaat cccagctact caggaggctg aggcaggaga 236580
atcgcttgaa cccaggaggc ggaggttgca gtgagtcgag atcacaccac tgcactccag 236640
cctgggcaac agagagagat cctgtctcaa aaaacaaaaa atggccgggc aaggtggctt 236700
gtgcttgtaa tcccagcact ttgggaggct gaggctggtg gatcacctga ggtcaggagt 236760
tggagaccag cctggccaat gtggtgaaac cccatctcta ctaaaaatac aaaaattagc 236820
cgggcatggt ggtgggcacc tgtaatccca gctactctgg aggctgaggc aggagaatca 236880
ctcgaacccg ggaggcggag gttgcagtga gctgagatca cgccactgca ctccagcctg 236940
ggtgacagag agagattctg tctcaaaaaa taaaacaggc tgggcgcggt ggctcatgcc 237000
tgtaatccca gcactttggg aggccgaggc gggcggatta cgaggtcaag agatcgagac 237060
catcctggct aatacagtga aactccgtct ctactaaaaa tacaaaaaat tagccgggca 237120
tggtggcggg tgcctgtagt cctagctact cgggaggctg aggcaggaga atggcgtgaa 237180
cccgggaggt ggagcttgca gtgagccgag atcgtgccac tgcactccag catgggcaac 237240
agagcgagac tccgtctcaa aaaaaaaaac caaaaaacca aacctacaca gttgtattga 237300
ggtgtaattc acatatcata aaatttaccc atttaaagtt ttttttggcc gggtgtggtg 237360
gctcacacct gtactcccag cactttggga ggccgaggtg ggtggattac ctgaggttag 237420
gagttcgaga ccagcctggg caagatggtg aaaccccgtc tctactaaaa atataaaaat 237480
tagttgggtg tggtagcaca cgccagtaat cccagctact tgggaggctg aggcaggaga 237540
atcacttgaa tctgggaggt agaggtttca gtgagccaag atcgcaccac tgcattccag 237600
cctgggtaat agagtgaact ccgccttgtc tcaaaaaaaa aaaaaagttt ttttaaagct 237660
attcacaaag ttgcgtaaca accaccacaa taactgttag aacattttca tcactccaga 237720
aagaaaccct gtacccacca gcagtcaccc ttcatttatc cccaagttcc ccagccttgg 237780
caactactat tccacttttc atttctacag atttgcctat tctggacatt tcatatacag 237840
caggggtgtc caatcttttg gcttccctgg gccacactgg aagaagaact gtcttgggcc 237900
acactaaaat acactaacac taaagattgc taatgagcta aaaaaaaaaa aaatcacaaa 237960
aaatctcata atgttttaag aaagtttacg aatctgtgat ggactacatt caaagccatc 238020
ctgggccaca tgagacccat ggccatgggc tggacaagct tgaaagatac atacaatctg 238080
tgggcttttg cgtctgactt cttttgaaga taaatgtttt caaagttcat ccatgtatca 238140
gatgtatctt tttattacca aataatattc cattgtatgg atataccact tttaacatac 238200
cccttcatca tctgagggac atttgggttg tttccacttt ttggctatca taaataattc 238260
tgctgtgaac atttgtgaac aagtttttgt gtggacatat gttttcattt tgtgggaata 238320
tacccaaggg tagaatcatt gggtcatgtg gtaactttgt ttaaactttt caggcattgc 238380
cagactgctt ttcaaagctg ccgcttcatt ttacattgcg attagcaaag catggggggg 238440
ttctaaatct ccacatcctc accaacactt gtgtttttaa aattattata gctatcctag 238500
tgggtgtgaa gtggtatctc attgtggttt tgatttgcat ttccctgatg actaatgatg 238560
ttgaacatct tctcatgtgc ttattggcta tttgtataac ttctttggag aaatatctat 238620
tcagaccttt agctcattta aagaattggg tgctcttttt attattgaat tcatcatcac 238680
tttttaaatt gagcagttac ctaaagctct acctaaagct ccatggtcag taaaattcac 238740
ccacatgaca gtggtatgtg aggcaccttg tagtatgtaa aatgtggaaa aaggccaggt 238800
gtggtggctc atgtgggtaa ttccagcact ttgggaggct gaggccagtg gatcacctga 238860
ggccaggtgt tcaagactag cctggccaac atggtgaaac cccgtctcta ctaaaaatac 238920
aaaaattagc caggtgtggt ggcaggtacc tgtaatccca gctacttggg aggctgaggc 238980
aggagaattg cttgaatgca ggaggtggag gtttcagtga gctgagatca cgccattgca 239040
ctccagcaag actccatctc aaaaaaaaaa aaaaaaaatt tggaaaaaga acgtcactga 239100
agcaatgtca tgatgcctac tgcttatgta tttctgggct ctgattccat ttatgatggg 239160
gatgaggcat ctctatttga tcagattagt attttgtaag ttagtaaacc actaaagcac 239220
taattagatt ttctcaaata tttgaaaaat atatgatttg atatgaaatt taataaatga 239280
tttagtcatc ccaacagttg aagacaataa aacttagtgt aagtataaat agaaccaagt 239340
tgttataata aaatccattg atgctttcaa gctatagcta acttgaattt ttacctctac 239400
atacatttat taaaagtaag ttgcaaaaag tttacctaaa ttccttctag ataaattaca 239460
aatgagaact accttctctt cttacaaatt tggtcttttg cacaacagca atggctaaaa 239520
taaatggggc caaatttttg gagaaagtct tgtttaattc agaatataat taaaatctca 239580
tggagcttta ttggttttag tatccagcag agaaaaaata ttgaaactcc gagagatttt 239640
gaattaaata gctacaacag ttttgtttta ttagttctga ctgtgcgaaa aaaaatcata 239700
aggacaactt tggaaaaatc tagttctttt agggccccaa atgactttta aaacaataaa 239760
acaaactata aaacgtacac aagtgatatt aaaaattatg tagttcaata aatgtgacat 239820
tcacaaatgt ggttgtaatt ttttttttta actttagcta agtgaacatg gatctgacga 239880
ttggatgtag aaaaggtagt gttatatagc ttaacagaat taagtgcttc agtttgccag 239940
tgaggattat gtagctgagt tggaatgttt taccaatgcc ttttaagatc tccatctact 240000
tcccaggatc tgtggctaaa tcaaagaaag agaggatgct tcttttatta gtgattattt 240060
aataactcta tgctagtgat tatttcccta gcattttaat atatatattt aaaatatttg 240120
tagatttaga tcttttggct ggtggctgaa tcatgcacag aataaaccag taccataaac 240180
taatactgtt tctgaagatg ggtaataaag gctggctagt gatttactgt gaaagcacta 240240
aatgaattgg agtcatcatc tgttactcgc agagtagaag gggcaggaaa aaggtgcaca 240300
atgaaaacaa aggggaaaca ttttgtgaga aaaataatta gcacagtttt ctgcttagtt 240360
tactgaggtc ccaatctttc aagacccaaa cataacttgt gaaggtaggt ttgaacttca 240420
tataataact gttctttctt tgtccccaga gcttgtctct gtccttggaa cacagaagag 240480
cacattgaat tcatcaggcc atgggatgag aatgatgacg gtggagatgg cacaacactg 240540
gagctgaccc acagagggat caatgattcc caaataaccc ttcactgata gtttccaggg 240600
gcaaggcact acctgaaatt gtatgcaatc ttcagaaatc attcaaaaca ttatgccttt 240660
aaaaaaaatg gatggtaggc cgggcgcggt ggctcatgcc tgtaatccca gcactttggg 240720
aggccaagat gggcagatca caaggtcagg agatcgagac catcctggct aatatggtga 240780
aaccactgtc tactaaaaat acaaaaaaat tagccgggtg tggtggcagg cgcctgtagt 240840
cccaactact cgggaggctg aggcaggaga atggcgtgaa tccaggagga ggagcttgca 240900
atgagccaag atcatgccac tgcactccag cctgggcgac agagcaagaa tccgtctcac 240960
acacacacac aaaaaaagta tggtaaaaag gaatttgaga ttaaaaaaag aaacagtgtt 241020
ttcatttttt ctcattttta tattgaaaac tttcaaatct acaggaatta tggtaattta 241080
attgctatgt gtaattataa aaaagctaaa attagagtac aacaaatact tgtatactct 241140
gaaattatag tacaacaaac acctgtatac tcttcaccta aattcaccaa ttaataattt 241200
tcgatacagg catacctcat tttattgtgc ttcacttcat tgtgctttgc agatattgca 241260
gtttttacaa attgaaggtt tgtgacgccc ctgcatcaag caagtctatt ggcaccattt 241320
tcccaagaac atgtgcccac ttcatgtctt tgtgtcacat ttgggtaatt ctcataatat 241380
ttattgttat atctgtaatg atgatctgtg atcagtggtc tttgatgtta ctattgtaat 241440
cgttttgggg caccacaaac tgcacccata taagacagct aacttaataa atgtcgtgtg 241500
ttctgactgt cctactcaca ggctgttccc cagtctcttt ccttctctcc ttgggcctcc 241560
ttattccttg agagacaaca atattgaaat tgggcaaatt aataacccta caacagcccc 241620
taagtgttca aggaaaagca agaattgcat gtctctttca atcaaaagcc agaaatgatt 241680
aagctcagca aggaggccat gtagaaagtt aagatccact aaaagttaga cagcgctgtg 241740
aacacaaagg aagagttctt gaaggaaatt taaggtggta ctccaatgaa caaatgaatg 241800
ctaaaaagtg aaacagcctt attgatgatc tggagaaagt gcaagtggtc tggatagaag 241860
atctaaccag ccacaacatt cccttacacg aaagcctaat ccagagcaag gccccaactc 241920
tcttcaattc tatgaaggct gagaaaggtg aggaagctgc agaagaaacg gtggatgtta 241980
gatgaggttg gttcacgagg tttaaggaag gaggccatca ccataagtgc aaagtgaagc 242040
agaaaatgtt aatagaaaag gtgcagcaag ttacccagaa gatctagcta agatcattga 242100
tgaacgtagc tacactaaac tgattctaca cgtagaccaa aaagccttct attggaagaa 242160
gatgccatct aggactttca tagctagtga gaagtcaatg ctggcttcag agtttcaaag 242220
gacagtctga ctctcttgtt aggtgctaat gcagctgaca tttaagttta aagcaatgct 242280
tattggccat tctgaaaatc ctagggccct taagaactat gctacatcta ctctgcctgt 242340
gccctataaa taaaataaca aagcctggat ggcagcacat cattttactg tgtggtttac 242400
tgaacacttt aagcctactg ttgagatcta ctgcccagga aaaaaatatg cctttcaaat 242460
tattactgat cattgacaat gcacctggtc acccaagagc tatgatggag atatacaaag 242520
agatgaatgt tgttttcatg cttgctaaca caacatccat tctgcagccc atgaatcaaa 242580
gagtgatttt gattttcaaa tcttattatt taagaaatat attttgtaag gctatagctg 242640
ccatagatag tgattcctct gtgggcaaag tcaagtaaaa cccttccaga aaggattcac 242700
cattccagag gccatgattc atgggaggag gtaaaaatat cattaacaga agtttggaag 242760
aagttgattc cagccctcac agatgacttt gaggggttca agatttcagt ggagaaagta 242820
attgcatatg tgctggaaac agcaagagaa atagaagtgg agcctgaaga tgtgactgaa 242880
ttgctgcaat ctcatgatac aacttgaatg gatgaggagt tacttcttat ggatgagcaa 242940
agaaagtggt tttttgagat ggaatctact tctggtgaat atgctgtgaa cattattgaa 243000
atgacaacaa aggatttaga atattacata gctggatgta atggctcatg cctgtaatcc 243060
caacatttag ggaggcagag gcaggagcat agcttgagcc cggtagatcg agacatgcct 243120
gggcaatgaa tggagcgaga ctccattctc tacaaaaagg aaaaaagaaa aaaaaaaaag 243180
gacaaaaaag agagactatt acacaaccat acttgataga gcagcacagg gtttgagaaa 243240
atgaactcca attttgaaag ttctactgtg gataaaaatg ctatcaaaca tcaccacatg 243300
ctacagagaa atcttttatg aacggagggg tcaacagatg tggcaaactt catggttgtc 243360
tttaaggaat tgccacagcc accccaacct tctgtgcctc ccaccctgat cagttagcac 243420
tcatccacat caaggcaaca cccctcaaaa gcaaaaagat tatgacttgc tgaaggctca 243480
gatgattgtc agcaattttt tttttttgag atggagtctc actctattgc ccaggctggt 243540
agtgtagtgg catgatctca gctcactgca acctctgcct cccgggttca agcactttcc 243600
agctaatttt tgtattttta gtagatacag ggttttacca tattggcagg ccggtcttca 243660
actcatgacc tcaagtgatc cgccagcctt ggcctcccaa agtgctagga ttacaggcat 243720
gagctaccac tccctgccaa tcgttagcaa ttttttagta acaaagtatt tttaaattaa 243780
ggtatgtacg ttgcttttta agacataatg ccattgcaca ctgaatgtag taaagtgtaa 243840
agataacctc ctttacatgc actgggaaac caaataattc atgtgacttg ctttattgct 243900
atgtttattt attgaagtgg tctagaactg aacctgtgat atatccctct ttatatatac 243960
atatatgaac atatacatag atatgtgtgt ataaatatac atgtagatgt gtatgcacat 244020
atatgtatat actgaagtat ttaaaagtaa gctgcacata tgacccatca ctcctaaaga 244080
ctttagcttg caactcccaa gatcgcattt tccaaagtaa cctgcaatac caatcacatc 244140
taaaagatta ataatgtcaa ctaacatata cttcatagtt tgtccccatt atcccccccc 244200
ccaataaaaa gcaatgtctt ttatagtttt tttttttttt ttttttttga gacagagtct 244260
ggctctgtca cccaggctag agtgcagtgg ggcgatctcg gctcactgca agctccgcct 244320
cccgggttca cgccattctc ctgcctcagc ctcctgagta gctgagacta caggcgccca 244380
ccaccacgcc tggctaattt tttgtatttt tagtagagac ggggtttcac cgtgttagcc 244440
aggatggtct cgatctcctg acctcgtgat ccgtccgcct ctgcctccga aagtgctggg 244500
attacaggcg taagccaccg cgcccggctc ctttatagtt ttttaaaatc cagaatccag 244560
ttaattggga tgatatgagg ccttgtgaaa atcctcatcc tcaacatttc acgtgttagt 244620
tgatgatgat tcttcttgaa ttattacgtt tttcgattgt accaattttt ctacatctgt 244680
tagctgacat tcttccagct ttcacatcca ctgcaccttt ctctctctga gtaccactat 244740
ggactcacga attgttatat tcaaagtggt ataatcccta atgtattttt aaagcttaaa 244800
tcaccccaaa ttttgccgat ggaaaccact ccaaggtggt ccctattttc ttttcacggt 244860
ttctgacaca agatgtcttt gagcttacct tgtacttttc ttgccctaga tctagaatca 244920
gccacttctt caaggactta cttatacgac gaataggtca tacatccatc tagtgcaagt 244980
ctccagatat tcacagctat acatgccccc tccaatccca cccctttctt catagtcagc 245040
caatgttcgt tatccttcca gagatagttt atatcatgga gtaatataaa aatttatgat 245100
ttatatgtac tgtataatag agcaattata ttcttaccta cccccaacat gctttaactc 245160
gaatgatagt gtagtacact tctgagtttg tctgggcttg tagctcttgt ctgtcattag 245220
attctgaaag atacctatta acaataccct aaaaagatca ggagccttta ggccaagtct 245280
caaacgtttc agatgagaaa aactcaggga tggtgacttg acggaagttg cattaactaa 245340
caacgctcac atgctattct ccggacttgg aatgtccaag caccctgggc ttgctgacac 245400
ccactcactt ttcaaacatc aattcttggg aagcttttcc cttccttaat gtcccccaga 245460
taggtgatgt ccccttgtta caggttttag accattatgt acatctcctt cctagcacat 245520
acatatatta aaatttgttt cctgtctcca tgaggagagg gactacatct gtccttttca 245580
ctgctggcac acagtagttg ctcaacaaaa atgtgcgaag tgaaactcga acaggcggtc 245640
aggctcacag tccacgcctc ttcctactgt ggtcccgctg tgtttggaca aatagttcca 245700
aattataaac tttaaaacct gagaagtctc aatagaaaaa gcagggaagt ctttccattt 245760
ccatgattaa tggaggacct tagcagaaac ggctcaccgc gaggtgtgac gaccgcagga 245820
gggcgtcaat caagaaaatg cccccttgcc ccggaggcga gtggagccgc acagagccga 245880
ggccatacgg gccagcagta cggactgctt caatagaaaa cacgtgcaaa accagagagc 245940
cagactgcgc acagctgcga ccctcaagag ccagcagaaa agacacggag cggcccccac 246000
tggattgaag actgcacagc ctctgcccgg cgctgtagga cccgggcctc cccttgattg 246060
ctgctcgcag cggcttctgc cagcatcctc gcggaagtgg cttgagtccg aggcctccaa 246120
atgaaaccag gtggagcggc caagagtccg cagcacctga actccagcca tccgaatccc 246180
gccgggccgc agctaagggc cgccgagcag ccctaggaac ccagggcact gtcccgctct 246240
gagctcggga aacggccggt gtgacagcca gcgccactgt taatccaggg agcgcaccca 246300
ccgaggaggc gtcttaggcc agcggcctct gctcccgccg agtcccgctg ctccccgcct 246360
ggaaacagct gctgccaccc cgggcccagg cctggacggc gcccccgaat ttcggcagag 246420
gtgcctcggg gccgcggaca gggccgctgg agcccgcact ccagaaggcg gtcttcctcg 246480
cgcctccccc gacggacgcg gcccagggct tccctcgagg gcgtcctcgg ccggggtgcc 246540
tggagtcccc aggggcgaca gccgcggcgg ggcaccaggg cgccagttcc ccggagcctg 246600
gtagcaagcg cgtcccgggg gcggggagct tggccggcgg ggcgggccgg ggtgggggcg 246660
ggcggctgct cttggggccc cgcgccattg gccgcagggc ccggcggcag gaaggggcca 246720
gcgagcgcgg ccccaccccg cggccggcgg agcctatcgc cgggagcgcg gagcgcggat 246780
aaatgtagcg ccgcggcgcg ggccagcagc tctgcgaggg gccggagcgc ggcggagcca 246840
tgcagtaccc gcaccccggg ccggcggcgg gcgccgtggg ggtgccgctg tacgcgccca 246900
cgccgctgct gcaacccgca cacccgacgc ccttttacat cgaggacatc ctgggccgcg 246960
ggcccgccgc gcccacgccc gcccccacgc tgccgtcccc caactcctcc ttcaccagcc 247020
tcgtgtcccc ctaccggacc ccggtgtacg agcccacgcc gatccatcca gccttctcgc 247080
accactccgc cgccgcgctg gccgctgcct acggacccgg cggcttcggg ggccctctgt 247140
accccttccc gcggacggtg aacgactaca cgcacgccct gctccgccac gaccccctgg 247200
gtaaggcggc cgggcgaggg tgggggcgag gaagcgccac ccggcaggtg gcagcgcccg 247260
ggaggccgag ggggcgaagg gggcaggcgg tagacggctg tggcaaaagc gatggaaaag 247320
cagctgtcgg gcacgcgcgg ggacgggagg cgcgcggccg gggctgaccg caccgtgact 247380
cagattggtt ttcctgcacc ttgcaggcgt cggggcgcgc gggccggcgg tagacctcgc 247440
ggcgagggca gccgtggacc gcgcgtgtta cccggggtgg ggctccctta gcgggaggga 247500
cggggaaagg ggcgtcgagg cgcccctggc cggcccccgt ggggcaggga tcgcagccgg 247560
gcccgggtgg ctacggggct ggacctggtt caacaggctt gtgcagtgaa agagcctgac 247620
cctttccgtt catacaggaa atcttgagtt ctcgatagga gtaaatctgg tttcagaagc 247680
tgttaagtgt tttcctggct gcatgaagca gacccttccc ccggagtagt gcacgctgct 247740
gattatttta tcgacatcct caatacagac aaattcagga aacactcgac ttctgatagc 247800
cgggatccgt ttttctgata ttgttcattt cctctgtgtg ggatgtgact ttatggcagc 247860
taaaataacc agttgcttcc tatttttttt cgaggctgtt ttgcaccagt ctgaagcctg 247920
acgcaagcaa aatctcgaaa aaacaatgct agctttcaaa gcgttgaggt gcaggcctgc 247980
agacggacgg acggaccgac cgacactact ttgggagtag gaagcagcag ctcctggtcg 248040
ccagttagga agggaagcag ggacacatag ggcatatatt ccagaacttc ggggcttgtc 248100
ccctcaatgt gcggcctaac aaataagccg cccgtggtcc gcgctgtggg tgacccttgg 248160
cgccttcgag gtctggagcc ctagggtaaa taaggaaacg gggcgcctct agagttttaa 248220
atgaactctg ttattggaag cttcagtagg gaccctgaaa acaattaacg tcttaattag 248280
cattttaatg tctccattat tacggcgcgg gctctagctc agccctttac cttaccttct 248340
caccgttaac aggggagggg gattgtattt ttagttcatc tttttatgtt tttgagttgt 248400
tatcctgtct gtctgattcc agcctcgagg gtttgatgat gcggcccgag cctggctgtg 248460
gtcgcctgtc ggggctggag cgggaccctc agccgggccg ggcctggggg ctaacgtttt 248520
cacagtgcgc cctgagtttc cttgggttac tgctgggacc gcgcaggagg aagcaaagag 248580
tttttcgagc tagaccaaca ggaaacacat tgacggaaat gttgccatag cccatggggt 248640
ggctttaact ggccgccccc gcgggctggg tgtgaaatca gaggaggccg cggctccccc 248700
ggccaggatt ggaggctcct cgcgcaacct aatgcgggtg tccgggcccg agcgcttccc 248760
gcgcagccag gccttgtcgg tgcagcagcc ccgctcctcc ccaacacgca cacacccggt 248820
gttcgcaagt gcggctcacc aagggagatc caagggggca aaaagttatg tataaatccg 248880
agagccactg gggaaagagg gtcgtggtat tgtaagccca gccggtagtg cccattcttt 248940
ttggggttaa aggtatgtct ggatttagtt ctaggtgtga cggctgtgtg tctgtctgtg 249000
tgtgtacaag gctgtgtgca tctgcggtgt cagtgtgtga ctttgggtat gtttgtgtgt 249060
cggtgggaac gtgttaggtc cacgtgccgg tgggtgtatg tgaatgtgtc tggttgggtg 249120
gcctcctggc ctacctttgt catccctggg gcccgacagc tctggggtct ggccaggccg 249180
ctccagggca gtgggtgagc gccgctcttc ccgctcgcag gcaaacctct actctggagc 249240
cccttcttgc agaggcctct gcataaaagg aaaggcggcc aggtgagatt ctccaacgac 249300
cagaccatcg agctggagaa gaaattcgag acgcagaaat atctctctcc gcccgagagg 249360
aagcgtctgg ccaagatgct gcagctcagc gagagacagg tgagctcgcg gggggcctgg 249420
ggccgcctcc ggggaaggga aggcttctgg ggtaggggtc gccgggccac cgagagagag 249480
gcgaggagcc accctgccct ctggcacgtc ccgacgcggg ctcgttgcaa gttttctttc 249540
tctctttcct gtaggtcaaa acctggtttc agaatcgacg cgctaaatgg aggagactaa 249600
aacaggtatg gacatggttc tgtttctatg gaaataaata tttttatcac gttatctcag 249660
tgcaaggctg ttatgggttg tatgcaaaag tcatttaaga gactatttaa cctcttcacc 249720
ttgattaaaa agacaaatag tcctgctgaa attcaggatg agttgctcag gtggcagtaa 249780
tgctaaaagc acctaatggg gttcctatat caattatgct tgggttttca cacactggct 249840
agtaaaactc ccagctaatt gttttataaa tcccatatgt tgtttatcta attaacgcag 249900
gaaagataaa gtaattgaca gtaaatgact taagcagtta tctttgggag aaaattgttt 249960
cttttattta cacagccata ataaaaccag gcagaactca agggtaaata taaagaaaca 250020
aacaccttta agtcttgttt gcactgtttt ttagaagaat taaatgtcag ggagtttaaa 250080
aattattttc tatctaatgc cagctcttaa aatgaaatat aaaacttgta agaaaatatt 250140
caagcctgtt tggaaagtag cttaaacttt ctgacagtct ttcttccagt gaccttgttt 250200
cgcaatatgt gataaaaagt aaaggctaat ttagttttta aaagaaggtc tattgactta 250260
aagctaaaca ttcatttttg atgtcctaat ctggatatac tctccaaagt tatctgataa 250320
ttgctgaatt gttcacttgt cattgtatat acggtttata ataaatactt tgaaacaatg 250380
agtggagaaa tttaaagcaa agtggatttt aaaatttatt gatcattact ttttcattaa 250440
aaagaaagtg tcccaaagaa tattttgaaa tagaaaataa aattcccata acttcattac 250500
tgtaatgtga tttaaatatt tgcagatcca ctctcaattt ctgaccgtgt gcatctattt 250560
gttgagacta taaggcttgc aactatataa caaatagatg ttgtaatatt tctatgtagt 250620
atttatatgc attttagaca agtataggat taaaaattca ctagcttata tacttaatag 250680
ttcttggtca aaggcttgac tctagtttac cagagacctt aaaaatagtg gtttctatgt 250740
aatatgggga tatttcccag aaatttgccc cagattttaa gtaaattgta gctaattgtc 250800
taccttctca gttggcagca ttcttccaga tccacatttt ttttctttgc catgattttg 250860
taacttgtta aacatttaca catgtagatg tttataaatt tactccagaa aatgaatgaa 250920
aaaagatcca gtctaataat tgaaacttta ttcttaattc accagttagc attcttattt 250980
ttaaaatgtc tgaaagactg ttaattggca aggtttaaat atttaaagaa tgaagttaag 251040
gtgtatctgt agtgatctga ttttctttcc cccttaaact tttgagaata ttgaaaccat 251100
gggtctgggc tggtgttaag taaccagaat ttgtgttcaa ttggtataat gtatctgccc 251160
ttatgttcat gactggttct catcttttaa gcatataatc tttgatatga cagaaacttt 251220
tcttccaaag tgagcaaaaa gcacaagtat gtatctcctg aataagatta taaactgaaa 251280
acactcagta aactaaaaat taaaagatgg aataggatga agagacattt tgatttattc 251340
ctctcaccta tccatatttt atgatccttc caatctccat tttcatttcc tttattcagg 251400
agaaccctca aagcaataaa aaagaagaac tggaaagttt ggacagttcc tgtgatcaga 251460
ggcaagattt gcccagtgaa cagaataaag gtgcttcttt ggatagctct caatgttcgc 251520
cctcccctgc ctcccaggaa gaccttgaat cagagatttc agaggattct gatcaggaag 251580
tggacattga gggcgataaa agctatttta atgctggatg atgaccactg gcattggcat 251640
gttcagaaaa ctggatttag gaataatgtt ttgctacaga aaatcttcat agaagaactg 251700
gaaggctata taagaaaggg aatcaattct ctggtattct ggaaacctaa aaatatttgg 251760
tgcactgctc aattaacaaa cctacatgga gaccttaatt ttgacttaac aaatagttta 251820
tgtactgctc ttaggttgtt ttgataaagt gacattatag tgattaaatt cttccccctt 251880
taaaaaaaca gttagtggtt ttcactattt ataaaaaatt aattttgaac tttttgttaa 251940
atttttaagt tatagcttta aaggttttaa taggaccttc ttgaacgact tttctgtaat 252000
ctgtttatct cccacttaat ggaaaggcaa aggggtaccc caaatccaga ggtgcctaca 252060
tttcaggcag ccttggagta ttttaaaagg aaaacattct ttacttttat atgacattct 252120
tatactgctg tctcaaatcc aaaaacattt cagagctctt gtctcagaga tgtgtgttct 252180
ttttgtcaga gatatggttg atgagaatct taaatgcttg ttttgcacta tcacttagta 252240
cctgtttgac caaggtgtta aggggatagt acctcccaat tcaagcagag aaactgacct 252300
gactaaagtt aatcgcagat gaactagaag tcacaggtta attaaatgta agtagattgt 252360
agatactgtt ttatatcaaa caatgtttat aatgtgtata tagaattgtt cactgtaaaa 252420
aaaatggcca aaatgtgttt tttttttaat aagtaacttg actataaaat aaagccgtcc 252480
gtgggacgac tgacctcgtt gcaagtctaa ttctttgatt ttaaaaagtc tgcacacctt 252540
ttcttgacct cctgggtgct tggcgcggtg ctgggctctg gggacaggaa gtgactgggg 252600
ctggatcttg ctggggctct cgttccgagt cccaggaccg cgggtgggtg cctggaacgt 252660
gggttggggc ctctcccggg ccgtttgcct tggggctctg ctcctgccgg cggattctga 252720
gcctgatggg ttcgagtccc gggcacgcca ggcgacggga agagccgagg cccaggcccg 252780
ctgcgccgtg aagcgcgggg ccgtccggac cccatcgatt ctcctcggca gccgggctgg 252840
ccctcgccgt ccttgaccgg ctgagtcaac accgcgtcat ccctcggcgg ccggggctcg 252900
ggaacccgct ccgcgcggcg gggcccgggg tggggaagtc cgagtggggg gatggggggc 252960
cctggccgag gggagcgggg aggtggtttg cgctgagaac tgttctcgaa cccggggaac 253020
ttcagaacct ccttcagggc ttgtggaaac ccagccctgt tcctggagtt ctgatctgtt 253080
aggactggac tgggctggga ccgggaattt gtatttccaa caagagctga ggtgacgcca 253140
aagctgcggg cccggacacc acccctgggg aaccactgat gtgagcgcac ggggtgcgga 253200
agaagcaggt gggaccggca ctcctcctcc cacctgcttt cccgggtaac tttggccagc 253260
aagaggctgg ggcggagggc tgagaggagg gaaaggcgag ggggaacaga ggaacgaggg 253320
gcttatccag gctagtggga gtgaggtttg atggctgagc agagggcgca gggtggagga 253380
aggggcacca tgaaatggag ggagaatggg gtgctgaggt taggtgggtg tgggggccca 253440
ggtttctgat tgttcagggt attgctagtc tgtgccagct gcttgcctag ctgagccatc 253500
aggtctctga cattccagga aaaccatgac agagatctgt tggggagagg cctggaaatc 253560
tctctctagc cttggcctcc atggaggagg cctctagttg tagagttggg tacttctggc 253620
taaggacctg tgaaggaatg acccgacccc tggcctggct tctagggctg tggcgccccc 253680
agatatggac actcagatga agacagtgaa aaagtcattg ctgttaggga gttgtcacat 253740
ccggcttcct tagattctaa gaaggcccct ggaattgcag gggaattaag atttctgaga 253800
aaagcagtac tagtccttaa gcagagaggt gtggatagac ttggaagttt gaagatttgg 253860
gttggtgttt tagttcttgg cctcaaatca tttgtagaac gaagatggtc tagttgaacc 253920
atatgaaccc cttccagctc tcaaattccc aactcagttc atgccgtcct tattgttctc 253980
cctactcccc atgtctcctc agccttggca cccaaagtgg ccacccagtg ggagagtttc 254040
ccttcccatc agctgtaggc tccagaccct gcctaaaaga ttacatcaag gtttcaaagg 254100
tcataatcca tctcgtctga aaatgggact gtccatatca gatttttgga gaatgggagg 254160
cttatggatg taatattgaa ttaaagtaat cctgaaacct gtgtgtgtgt ttcgcttctg 254220
gagataaacc ctagttaatg tttattcttg agagtaactg tatcatgact attcagcaga 254280
gaaatacaga ctaatgtatt gattcacact agaaaacttg aggggtaaaa tctagtttta 254340
aagttgacaa ttccctgaaa ccaggctgct ttccaatttc aagcttatcc taactctcac 254400
caagttgctc ccattctgaa tctaaacatg tccttaagaa tcaagagcat gttaactgga 254460
ggaggtgcag tattctcagg acacaggtag gcattcactt ttctgaagta ccccattgca 254520
aattgccaat gaagatttag aaattcctcc tacgatccgg gcatttttca tttgtttgtc 254580
ttttggctat ttcatgttga agcagcacgt atgaccttta cagcacaact cctgtcttgt 254640
ttttaactct ttttatcagg agattcaact tgaaaacggt tatcatttag cagaaagaca 254700
aacagaaaag cacatttttg taagcaaaac gttaatgatt tgaagaattg ccctaatttc 254760
accatgacag tgtgtgatta atcccctggg tcgtgtactt atactttaag ttattcagtt 254820
cattggtctt tgatattcag aaccctttct aacttaatat gatattgatt ttgttggatg 254880
atgtactttg actctttaaa caacatcact tttagagatg actagcctac tgtgtagtat 254940
tttatttaga gaactattgc ataacatttc tttttcttac tttttatggt tttctcaggg 255000
tgaaagctga taagacatct tgtgggaaaa gaggaaacca tctctgatag tgaaagtacc 255060
ttttctcttt caacttttct gacaagcttt tgtgtgtgtg tgtggttttc attactgtga 255120
atattcatct tagttaatgg gggcaggaca gggattcaag aaaacttatt tggacttggg 255180
gaaaacgcat tttgctgggg acagaagaga cttactaaaa ttcccactcc agatatattg 255240
gcatgtggaa gctgatgaac ccaaaagctc ctgattttga ttttttttca agatctaata 255300
atcaatgatc ctttaaatct gtgattggag atacttatgt gactacttac aatatggggc 255360
agaatgtgaa taccaaacaa tcacccagga tgcttctaaa tgattgggag ttcaagccag 255420
gcactttggg agaccaaggt gggcagatca cctgaggtca ggagtttggg acaagcctgg 255480
ccaacatggt gaaaccctgt ctctactaaa actacaaaat tagctgggcg tggtggcacg 255540
tacccgtaat cccagctact cagtaggctg aggcaggaga atcgcttgaa cccaggaggc 255600
ggaggttgca gtgagcggag atcatggcac tgcactccag cctgggcgac agcgtgagac 255660
tgtgtctcaa aaaaagaaaa aaaggctggg cgtggtggct catgcctgta atcccagcac 255720
tttgggaggc cgagccgggc ggatcacgag gtcagaagat caagaccacc ctggctaaca 255780
aggtgaaacc ccgtctctac taaaaataca aaaaattagc tgggcatggt ggcgggcgcc 255840
tgtagtccca gctactcggg aggctgaggc agaatggtgt gaacctggga ggcagagctt 255900
gcagtgagcc aagattgcac cactgcactc cagctgggcg acagagcgag gctccatctc 255960
aaaaaaaaaa aaaaaaaaaa aaaagagaaa aagaaaaaga aaaaataaat gattaggagt 256020
tcagtatttt gtggtcaaag taatcaggtt gtactgacat ttatttttca gtccatagag 256080
gttttaggaa gtcccagttg ggctctgtat atgtctaatc taattttgtc ccttgatatg 256140
tattttttgg tattgttgat aactccaaat aagaagcact ttggagactc ctcccactca 256200
cccaaagatc tgagacccca ggtctacaga ggagcagcta ccccttcaca tgtggctgta 256260
ggaaaaccag aatcaccccg ttgtggatac aagcatggct agcagcaggg gagatggaac 256320
tctctacccc tctcttgctg gctggggccc cgggggacac ccgcatcctc agcctccggt 256380
ccctcagttc ctagagcgga gcgtggctcc cccgagagga ggcggcgtcc ctggcacgcc 256440
gtgggccccg agtgtcagtc agaaaagcta gacaccccga gtgattccct tcttttgaga 256500
cagctcttgg gttcgcagtt gcaggcggga aacctcttca gtcggatgta cacaaatctc 256560
tctggataac acagagggct gctgcgaggg gaccttggag ctggcagaga catttcctgt 256620
ctgcggagga tcttgacttt ggagcatccg gccgtcgccg cgagcggccg aacactttcc 256680
tgcgtcgctg ctttaaaaaa aaaaaaaaaa aaaagtcact gccaatatca ggcgactttc 256740
cacatgaaag ccaatgaggc cgcttcccac ttaaaaaacg tttgtttaaa acgtattttc 256800
taagcattcc ttagactccc ttttattttg gagggggaat ttggtatctt tgaaaaacct 256860
taagtattca aaaatctcat tctctctgaa gacaacttct tagatgaaca agtacgggaa 256920
acagttaaca gcagagggtc cctcccctgc tgctcgcaga cgctcgcagt aagatatgct 256980
cataatcttg tttatgtttt tctttcatgg tttatgccct tccatccttc ttctgctccc 257040
cacttttcct tccttattca ttccctttct cttttttccc ctttcccttt taggattcct 257100
catcttcatt cagtttactt ccccatttag aatagaaaat gactaatctc tactctttag 257160
atgtcactat tggggccaag attttcttta aatttataga aatgttcaac ttttagctga 257220
ggtgagaatc ctgtttgagg gaaatgttgc tggagtccta atgcgttgct cccatcccgc 257280
cagggatagt tttttttttt tttaagccaa gataagcaat aataataaca tagtccaggt 257340
acagtggctc atgcctgtaa tcgcagcact ttgggaagcc gaggcaggca gatggtttga 257400
ggtcaggagt tcaagaccag cctggccaaa atggtgaaac cccgcctctg ctaaaacaaa 257460
tacaaaaact ggcagagtgt ggtggcaggc gcctgtaatc ccagctactc gggaggctga 257520
ggcaggagaa tcacttgagc cactgcactc cagcctgggc gacaaagtgg gactccgtct 257580
caaaaaaaat aaaaaataaa aaataaaaaa ataaaataac ataaatagaa gagtaataat 257640
ccctgtgtct ctagggtcca ggcgctcagt agcactggag tgtaatggag ctgacagaga 257700
gagatgctgc aataagggat ggacagcaga tgctgtggca gagtctgcca gggactccca 257760
accagagaaa aggaaaagga agcctgttcc acggagaagg atgatcctgt acacaggtct 257820
ggaggtgaaa gagggcaggg agtatgcatg actggactcc agagattttg gatttcagaa 257880
ttttcagtga gggattgtgg acctgtgttc tctctaccca ccacacaggt ctctttgttt 257940
ttgcattggc actttcctct ctctggaact ctctttcccc caaatatagc ctcatggctt 258000
gctccctcct ctccttcaga catggccttc ccttgccacc tttttacaat gccaacctcc 258060
tcttcctatc cccaacaaca atttaatttt ctctatttca cttaatgcaa ttggatataa 258120
tatttttact gccttattta ttatgtgtct ctccttattg acatatgaac atcatgaggg 258180
tagggatttt tgtctgttat gttcactgtt atgtatcttc agcctgacct attaggtgct 258240
tgttaaacat atgttaaatt agtgaattac tgagcacgta ctatgtgctg ctaaatatta 258300
tgtaagtctt tcacctgctt tatatcttta tatcatttaa tcatcaagga ccctatgagg 258360
tagaggctgt tatcaacccc attttctaga taaggaaact gtcaccgaga aattaagccc 258420
aaaacctcaa ttactatacc gatcaatgtt gtaatgagaa cttcctcact gaatctgttg 258480
cattcttgaa aaggtatcaa atcaagtcta actctagcta taagaagaga attgaggttt 258540
tttgctcatc ttacctaatc ttttgccttg attcaaatct agcattacat ctggaaggga 258600
ctatgataca gtataaggga taagcaattt ctgtgtatgt aacatattat tctattacat 258660
taggaaacta ataagctgag tgaagtacca tttccctctc agttttctgg ggaaatgagc 258720
attataagat acactttcta tctttcaaag cgttaaaaaa aacaagttgg ccaggtgtgg 258780
tggctcatgt ctgtaatccc agcactttgg gaggccgagg caggcagatc atgagatcaa 258840
gagatcgaga ccatcctggc caacatggtg aaatctcatc tccactaaaa atacaaaaat 258900
tagccgagca tggtgttgta ctcctgtaat cccagctact cgggaggctg aggcaggaga 258960
atcgcttgaa cctgggaggc ggaggttgca gtaagctgag atggcgccac tgcactccag 259020
cctggtgaca gagtgagact ccatctcaaa aaagaaaaaa aaaaaaaaaa aaaaaaaaaa 259080
tatatatata tatatatata tatacatgaa agtttgcttc tcatctggcc cattgaggca 259140
tgttccacaa gggactctga acatagcagt gtgatagctt actatttgtg tccccagcat 259200
ccagcagtat gtttggccta cagtaaactt ttaataaata tcgttctaca gaatgaaagt 259260
gaaagccatg tgccctccct gaaggagagg agagccattt ccctccctcc ccaagccccg 259320
caccctcatt atacttccta ttccttggcc accagaaaca tagcttgcct ggatggcttt 259380
ggcagcacag cagaaagcat agtgggcagg ggctgtaagt gctacagaga cacttgacag 259440
gtgccaactt gggccacggg tgaggcgagg acatatttag gtctgtttaa gcctaaaaaa 259500
gctctaaaag aaaatgttct cttccatctt tttaccagca taaatttgaa gaggaagtgc 259560
ttctgtggtt ttattggcca tttttccctt tcagacttgg caatttcatt caatttccag 259620
ttcacacttc tcaatttcaa tcaagagttt tatttaaaat atatccagtt tactgaaaaa 259680
aaaaatgaca ctatttggga tgggctgtga tcactactat ttttatcagc atcatctatt 259740
cagaaaatgg aagcaggaaa tccaaatttt ttaaatgatt tctgaaagta ttttatgaat 259800
tgcaaagaag tgtagaagga agagaaagtg tttactttac atgctctttc aaagtttcaa 259860
ctttccttag ctgcttatgg aaactgcatt actacagact ttccattaat tgtagattag 259920
accagactaa ttcaataaat gtttaaaaaa tggaccctga gtgcaggttc agacgtccag 259980
aggaaatgac ttgatggtac ggaggtggaa gggagtgaga agttatgatg atgtatggtg 260040
ctgttgatca gttaattgaa ggtaaaattt ttagcttcac caagcaatta cctgatctca 260100
ttaaggtctt taaaatcacc taagtaccat ttgcctgagg cagctgcccc ttgtttagat 260160
gttatttcag gcattaagaa aaacacaatg ttaatggtga ccagggaatt tatgcatgca 260220
gtttctagta tatattattc tgaatcttag ccattattca gtattatcca ccttgtataa 260280
acatggggca ctaaaatata tttacatttt gtatatattt acatttcata tttgttttac 260340
ttttcaaatg tattttaaag catcatgttt acaagatagt tttttttaca aataaaattt 260400
gttaagagat atggattgtc aatttttttc aagaaactac ttattaaagt ataatatata 260460
tattttaaaa gctcacaaat cataggcgta cagcttacaa catgactaca ctatgtaact 260520
ggaatccata tcttgaaata gaacattacc agatcccaag gagctctctt tatgccccct 260580
ctagttcact atccccaacc ccactccaag ggactccact aacctgattc caaatggcat 260640
agattcattt gccaattttt gaactttatg aaagtaaaat cccacagtat tttctctttt 260700
atctcccatt cctcctccat tcctttagtt ttcaggtttt gttttgtttt gttttgtttt 260760
tggagacgga gtcttgctct gtcacccagg ctggagtgca gtggcgtgat ctgggctcac 260820
tgcaacctcc gcctcccagg ttcaagcagt tctcttgtct cagcctcccg agtagctggg 260880
attacaggcg tctgccacca cacctggcaa atttttgtat ttttagtaga gatggagttt 260940
caccatgttg ggcaggctgg tctcgaagtc ctgacctcaa gtggtctacc tgcctcagcc 261000
tcccaaagtg ctgggattac aggagtaagc caccgtgccc ggctgcccat tcctttagtt 261060
ttttttgctg gtaaattcat ccacgttatg gtgtgatgtt gtttgtttat cctcagtgct 261120
gcaaagtggt ccattgtatg aattttcatg ttagtttaaa taatgcatct aaatataaaa 261180
actgtgccat aaaatttaca tttgaaaatg acactgctca cattttactg gactccaaga 261240
ctcctaggtg gagagaaaga aagacagcag aaagagcagg agcatcaggc tgtcaagaaa 261300
caaatcactg caacctttca catatcagaa gggctgggaa tatcagtaac caatatctca 261360
cttatttttc ccactcaggt gggttaaatg aaaaaaatca ccgaggccgg gtgcggttgc 261420
ttatgcctgt gatcccagca ctttgggagg ctgaagcagt tggatcactt gaggtcagga 261480
gttcgagacc agcctggcca acatggtgac acccatctct actaaaacta ccaaacaaca 261540
acaacaataa caacaacaac aaaaccccta aaaattagct aggcatggtg gcaggtacct 261600
gtaatcccag ctactcggga ggctgaggca ggagaattgc ttgaacctgg agggcagagg 261660
ttgcagtgaa ctgagatcat gccactgcac tccagcctgg gcaacaaagc aagaatccat 261720
ctccaaaaag aaaaaaacaa tttctaaaga caacttcaat tctggacata ggtctccctg 261780
tagttacagt attttatttc agtagtttta ggaatttagt ttgcattcta ttccactttc 261840
attttcctgt tactttgatg tttaaaatta taaagctgct atgggatgat gtgctggatt 261900
ttatgtctag atttgctgtt aagtagcaat gtgactctgg agatatcaat tacctctctg 261960
tttcctcatc ataaaatgag gctcttggac caggtgattt tttttttttt ttattttttt 262020
ttatttgaga tggagtctca ctctgtcacc cagtctggag tgtagtggcg cgatctcggc 262080
tcactgcaac ctccacctcc cgggttcaag agattctcct gcctcagcct ccggagtagc 262140
tgggattaca ggcacgcacc accgcacctg gctaatattt gtatttttta gcagagatga 262200
ggtttcacca tgttggccag gctggtctca aactcctgac ctcagatgat ccacctgcct 262260
tggcctccca agtgctggga ttacaggcgt gagccaccgc gcccagccta ggtcattttt 262320
aatgttgcac ttaacactaa gacgctatgt ttttctgttc tatatctttc taagcaacat 262380
attcacccct aaacttcagt ggctatgtgt tttaggtgtt gagaggaatg aagaatcttt 262440
tatttcaccg gtcttctggt gttcactccc tgagcaggac ttgtcttata cttaagctca 262500
gttctaaggt taaatcaagg ctcagtctct atcaaagcct ataaaagctc accctaggga 262560
agtgttctta gggaagcatt tctaatattt ccagctgtcc atatattttc aaacaaataa 262620
tagggtattg aagtaaactc gaatgttgat tatacgtttt ctatcaaatt attcaagtat 262680
tcattcagaa aatatttatt gagcacctac aatgtggcac gtggaagcac tggggatata 262740
aatttgcata aggctcagta ggatgacttt gaagaccaga gaaaacatcc ttttaatttc 262800
caacattcaa ggatgaacct attttcaccc taagcataat tataattttg cttgctatat 262860
aataattcca tgctgtttaa gttcttgagt ttgtcttgca aaagcttttg ccaaacaacc 262920
catagcaaaa tatttaataa cacatgggca ttccaaagtc ataaataaga aactaagtgg 262980
acctcctttt agagcatgtc cccacaaatg ccctcttctc tcctcatttc catcctgggg 263040
tgaatgccca cttcacctcc cacacagtgt ggtttaaata actataaaat gtctccaaca 263100
gatggtatta cctggcatcc cacacccagg actgtcatct ctccctcaac atttccttcc 263160
tgtggcattg aggctgtgaa tctgcccatc tcccctggtt ttctccaggt aactccacct 263220
tgggcaaggt ctgcattata tatatatcta tatttacatg tataaaagta tatgtatatg 263280
tatttttaga ggcaaggtct cgctctgtca cccaggctgg agtacagtgg cataatcata 263340
gctcactgca gcctccaact cttgggtgca agggatcctt ccacctcagg cccacaagta 263400
gctgggacta taggtgcatg ctaccacact tggctaatta aaaaaaaaaa atttatagag 263460
gtaagatctc tatgttgtcc aggctggtct cgaactcccg tcctcaagca atcctcctgc 263520
cttggcctcc caaaatgctt gggattacaa gcatgagcta ccctttgggc acataatgta 263580
ttattaggta gagatggcaa aatttcttaa ttctcttgtt gggttaatgt tgttttgttg 263640
ttataaaatt tctaaaatga gaatgttttg cctccatttc agtttaattc cttctagagt 263700
atattcatag aaaatgaatc attgagccaa agggatgaat atttgataaa tattgtgaaa 263760
tacttcctgg gaaagttttg tcaatttata tcagtggaga acaaggatca tttctccata 263820
tcttcaccaa tggaagtgct gtttttctaa tctttcttaa ttatttagga gaagaatagt 263880
atgctgtatt ttaattttac atttcattcc tttcattatt cccaaagttg aattttaaaa 263940
atatgctggc caggtgctgt ggctcacgcc tgaaatccca gcactttggg aggccgaggc 264000
gggaggatca cgaggtcagg agatccagac catcctggct aacacagtga aaccctatct 264060
ctactaaaaa tacaaaaaat tagccaggtg tggtggcacg tggctgtagt cccaactact 264120
tcggaggctg agggaggaga atcacttgaa cccgggaggt ggaggttgca gtgagccaag 264180
atcacgccac ttcactccag cctgggcaac agaacgagat tctgtctcaa aaataaattt 264240
aaaaaaatgc taacctttta tttctttttc tatttgaatt cttttatttt ttattatttt 264300
tattattttt tgagacagtt cagtctgttg cccagactgg agtgcagtgg tgccatctcg 264360
gctcactgca acctccacct cccgggttca actgattctc ctgcctcagt ctcccaagta 264420
gctggaacca caggtgtaca cccccatgcc cggctaattt tttgtatttt tagtagagat 264480
ggggtttcac cattttggtc aggctggtct caaactcctg acctcaggtg atccacccgc 264540
ctctgcctcc caaagtgctg agattacagg cgggagccac catgcccagc tgatttattt 264600
gaattcttta tgttggaaga tataagcttt ttgccttatt tattgcaaaa atgtttctca 264660
gttatttaaa aagatattta ggaaaatgga acatttttgt gtagtcaaat gtaacaatat 264720
ctcttttatg atttctttct tttctcgtaa gcttagaaag attccctcat tcagaaagca 264780
aatgaacatc cacatacatt aaacaaaaac cctcccccta aaactctttc atctctcccc 264840
cacccctcat tattttacat ttaatgctta atctacctga aatttatttt gctccacaat 264900
atcaggtgaa gaactaaaat aaaaaattac ccctaaatac ctaactaagt gttccattca 264960
tgcaataatc attccttttt gcccttttcc tggattaaat gtctaaaaac accttggttt 265020
ctggactaat tgcaatgttc tttcaatttt gtttttgtat tttgtatatc tgtggctggg 265080
cttaaggact agaatttgtt ttctgtagca gaaggatgag agcttctgtt cattcttaca 265140
tataatcaga tttctccttc ttcgaaggtt gagttggctt ttgaaacaaa caacagctag 265200
ctttctcatc agagtctacc atatctgggt tttcctatgc atgacttttt tttttttttt 265260
tttttttttt tttgtgagac tgagcctctc tttgttgccc aggctggagt gcagtggcgt 265320
catctcggct tactgcaacc tccgtctccc ggattcaagt gattctcctg tctcagccta 265380
ccgagtagct gggattacag gcgcctgcca ccacgcccgg ctaatttttg tatttttagt 265440
agagatgcgg tttcaccata ttggtcaggc tggtctcaaa ctcctgacct caggtgatcc 265500
actcgcctca gcctcctaga gtgctgggat tacaggcgtg agccaccgtg cctggccttc 265560
tgttcatgac tcttgcactg cagatgaaag tgctctcaga acctacagag caccaaactc 265620
aagaaacaga acttaggaag ctttatgtgc atcattggct gaatcctctg catggaggag 265680
caagtgccct gcctgctagt ccatttctat tttaaattat agtgtaccac tccagtggtg 265740
tattagaaaa agtctttaga tggccaggca cagtgactca tacctgtaat accagcactt 265800
tgggaggcca aggcaggcag atcacctgaa gtagggagtt cgagaccagc ctgaccaaca 265860
cggagaaacc acgtctctac taaaaataca aaattagccg ggcatggtgg cgcatgcctg 265920
taatcccagc tactcgggag gctgaggcag gagaatcact tgaacctgcg aggtggaggt 265980
tgcagtgagc cgagatcgtg ccattgcact ccagcctggg tgacagagcg agactccatc 266040
tcaaagaaaa aaaaagaaag aaagaaagaa agaaaaagtc tttagattta aaaaatagct 266100
aacacttact ggagacttat attttttgca tttttttcaa atctttatga atgtcttcag 266160
taaagttaaa tattttcttt atttaataca tactcattaa aaattttcaa gtttattgag 266220
gtgaaatata acataaaatt agccatttta aagtgaacaa ttcagtggca tttagtacat 266280
tcaaaatgtt gtgcaaacac cacctttatt tgttccaaaa gattttcttt tcttttcctt 266340
tttttttttt ttttgattta gcttttgttg ctcaggctgg agtgcagtgg tgtgatcttg 266400
gctcactgca acctccacct gctgggttca agcgattctc ctgccttagc ctcctgaata 266460
gctgggatta caggcaagtg ccaccacacc tggctaaggt tttgtatttt tggtagagat 266520
ggggtttcac catgttggcc aggatggtct cgaactcctg acctcaggtg atctacctgc 266580
ctcggcctcc caaagttcag ggattacagg cgggagccac catgcccggc ctgttccaaa 266640
acattatcat ctttccaaaa taaaactctg tatccattaa gcagtcactc cccattctct 266700
cttctcccca ttttctggca accaccaatc tgctttctgg atgggtttac ttattctaaa 266760
tatttcacac aaatggaata atacaatatg tgatctttcg tgtcttattt ataaaacatt 266820
agcataacgt gtttgaggct catccacctt gtagcatgta tcagtacttc attccttttt 266880
atggctggac aatatttcat tgtataaata aaacacagtt tatttatcca tttgttagtt 266940
gatggacatt tgggttgttt ccaccttttg gcaattgtga gtagtgctgc tatgaacatg 267000
tgtgtccaag tacttatttg agtatctatt ttcaattact tgggatatat gcctaggagt 267060
gaaattgctg ggccatatga caattgtata tttaactttt tgaggaaatt ctaaaccatt 267120
ttccatcatc actgtgccat tttacattcc caccagcaat gtttgaggat tccagtttct 267180
ccgtccttac caatacttgt ccttttatag aaaaattata ggcaagctca tgggtatgaa 267240
gtggtatatc attgtggttt tgatttgcat ttctctaatg actaatgatg ttgggcacct 267300
tttatatgcc tatttgcatt tgtatacctt ctttgctttt ttatttttta tttttgagta 267360
gtcaagtgca gtagtgagaa gggggaaaga gtagaacaag gagttcgatc tataactgac 267420
tgaacaatca actgagataa ctcactatgt tcagaccagc ctgtatacct tctttggaga 267480
aatgtctttt caaattcttt actcatttaa aaattgtgtt gtttgtcttt tttgttgttg 267540
agttgtagga gtttgttaca tattctggat actaaacgat tatcagatat atgatttgca 267600
aatatttttt tcccattctg taggttgtct tttcactttt taaaaattga tacacaacaa 267660
ttgtacttat ttagagggta cacatgatat tttgatatgt gcatacaatg tgtaatgatc 267720
aaatcagggt aactggaaca tccatcacct caaacattta tcatttcttt ttgttgagaa 267780
cattccacat cttctagcta ctttgaaata tacaataaat tattgataac cattctttca 267840
cttttttgat aatgtccttt tttgcataga agtttttaat tttgatgaag ttccatttct 267900
caatttttct tttgttgctc aagcttttgg tgtcatagtt aagaatcaat tgtcaaatcc 267960
aaggtcataa agatttaatc ctatgatctc ttcaaagaga tttattattt tagctcttat 268020
atttgagttg ttgattcatt ttgagttact ttttggatat ggtatgaggc agcttcattc 268080
tttttttttt taatgttttt tttttttttt gagacagagt ctcactctgt tgcccaggct 268140
agagtacagt ggcgtgatct cggctcactg caacctcggc ctcctgggtt caagcaactc 268200
tcctgcctca gcctcccaag tagctgggat tacaggcatc tgccaccagg cctggcaaat 268260
ttttgtattt ttagtagaca caggtttcac catgttggcc aggctggtct tgaactcctg 268320
acctcaagtg atccacccac ctcggcctcc caaagtgctg ggattacagg cgtgagccat 268380
catgcccgcc caaagcttca ttgttttgca agccgatatc caattgtcct agtaccactt 268440
atagagatta tgcttttccc attgaatggt tttagcaccc tgctgaagat caattaacta 268500
tagacatatg ggtttatttc tgtactttct ttttttttat tttaattttt tttgaaatgg 268560
agtctcactc tgttgcctag gctggagtgc agtgacgtga tctcagctca ctgcaacctc 268620
cgcctcctgg cttcaaggga ttttcatgcc tagcttccca agtagctgag accacaggta 268680
cacgccacca cgcccagata atttttgtgt ttgtggtttt tttttttttt ttttgagaca 268740
gggtcttgct ctgtcaccca ggctggagtg cagtggtgcg atcttggctc actgcaagct 268800
ccacctcccg ggttcatgcc attctcctgc ctcagcctcc tgagtagctg ggactacagg 268860
tgcccgccac catgccccac taattatttt gtatttttta gtagagacgg ggtttcactg 268920
tgttagccag gatggtctcg atctcctgac ctcaggtcat ctgcccgcct cagcctccca 268980
aagttctggg attgcaggcg tgagccatcg cgccaggccc tgagcttatt ttcttaagaa 269040
ttacatactg tctctgtcaa agaggagaga taattggtgc ttaatacatt gtttacttta 269100
ggtttacaaa aggttagata aacaaaaaga ctaaaaatct gtttgtacta gtgaacattt 269160
ctgaattggt aacccacagt taggttgata gatatactga aagcaatttt tcttgttcac 269220
atgtagtact taatttttgt caagtttgtg actgctcagt tttgcatcct cctttgtctt 269280
cctcttttca tgaccttatt tcttcagatg gatgttttga ggttattttc tattttgaga 269340
aaactataca aatctgattt attatcacaa tagctcaaaa gtagttcctg ttgaagttct 269400
ccacattgtc aatactgtga cctcctgtca gtctcctaca ctggatggga aactcaagct 269460
gagatttctc caaagtgctt cccacagcgg ctgaactaat ttcaattacc accagtggta 269520
tacaggcgtt cccttttctc cacagcctcg ccagcatctg atgttttttg actttttaat 269580
aatagctgtt ctgactagtg tgagatggta tctcgctgtg gttttgattt gcatttctct 269640
gatgactagt gatgttgagc attttttcat atatttgttg cctgcttgtg tgtcttcttt 269700
tgagaagtgt ctgcttatgt cttttgtcca ctttttaatg tggttatttg ttttttgcct 269760
gttgcattat ttaagtttct tataattttg gatattagac ctttgtctga tgcagtttct 269820
gagtattttc tctcccattc tgtaggttgt ctatttactc tgttgatagt ttcttttgtg 269880
tgcacaggct ctttagttta attaggttcc atttgtcaat ttttgttttt gttgcaattg 269940
cttttgagga cttagtcata aattctttcc caaggccaat gttcagaatg atgttttcga 270000
gattttcttc taggattttt ataatttgag gtctaacatt taaatctttt aatccatctc 270060
gagttaattt ttatgtatgg taagagggaa ggatccagtt tcgttcttct gcatatggat 270120
agccagctat cccagcacca gttattgaat aggggagtcc tttccccatt gcttattttt 270180
gttgactttg ctgaagatta gatggctgta ggtttgtggt gtcatgcctt ttgaaggttt 270240
ttttttttaa tttttcactt agcacaatgc atttgaggtt tatctgtgtt catatgctta 270300
ttaatagtcc ctttgtttct gagtagtatt ccatagtatg gatgtagctc agtttgttta 270360
tttatttgct acttgaagaa catttgaatt gtttccagtt ggtgattaca aataaagcag 270420
ctataaacag ttcacttaaa tgtatttgtg taaacatatg ttctcatttc tcttgggtcg 270480
gtatctagga ataggattcc tgaattatag gataaatgaa ggtttaattt aataagaaac 270540
cgccaaacat tttcagtttt gtagagccac cataatgaat taccatggtc ttagtggctt 270600
aaaacaatag aattttattg ccttatagtt ctgtgaggct cgaagtccaa attcaaaatg 270660
ttggccagcc catactccct ctgaaagctc taggagagaa ttcttccttc cttttttttt 270720
tcctgagaca gacccgctct attgcctagg ctggagtaca gtggggcaat ctcagctcac 270780
tgcagcctct ttctgctggg ttccagcaat actcctgccc caggctgcca agtagctggg 270840
attacaggtg cccaccacct tgcccagcta atttttgtct ttttagtaga gacagcattt 270900
cactgtgttg gccaggctgg tctcaaactt ctgaccttaa cctgacctta agtgatctgc 270960
ccgccttggc ctcccaaagt actgggatct ttggggtatc gattttcttc ctgtaaacct 271020
ttgtggccgg tggcaccttt gcctgacttc ttgtgtccag gaagaatgag gtacacagac 271080
aagtgaaagg tgaagaagtc aaaggggagc tttatttagt gttagcacag ctcagaggag 271140
acccacagtg ggtagctcct ctctgtaggc aggttgtccc atggagtgtt cagctctcag 271200
cagagacgag gccctggaga gggtagctcc tctccacagg cgggttgtta agagcatctc 271260
tgctctcagc agagagggta gctcttctct gcagctagtc ttgccattgt ctctccatcc 271320
tctgccctct tcttgctgat cccagggctc agaggggagg aagtatgtgt tgattggtcc 271380
atgggcagcc ataggtgacc tggaggaggc acaagtcccc actccagtct gcgggactgg 271440
cagcccagcc cccagccttc aggccctctc tggcctgaag gtgtggcctt actggaacct 271500
gctcccttcc ctccaggaat ctgtctgcct tctgctgcca ttcatgaccc cggggctcag 271560
ccccaaccct gcttggagat tggagcaggc actgggagag gagaggccag gcaatgggag 271620
cagacaaccc caggcctgca ggtatgtttg gggaggggcc ttcttgggcc cccgcgggtg 271680
caggctgcag agatgcctgg gtcctgtgcc tgggagggca gctgcagctg cactctggga 271740
gctaccaccc ctccaacttg gaagcctctt cctctgcttg tccctggctc ctgcctgctc 271800
tgtggagcag gaggcccagg tctacagctg caggtccagg ggctgcagtt gcacccagga 271860
gggcagatcc tgcctgctcc tggccaccct caagagtgag gggaggcttg cgtccacagc 271920
tgcagtttgg gtgcccgtat ccccacccag gggggcaggg cccctgcctg ctcagtagag 271980
caggaggcct gggtctgtgg ccatggtcag ctgccgtcat tgggatgaca gtcatgagcc 272040
actagcctga cccttccttc ctcttctcac cttctggtag ctcctgcaaa ctttggaatt 272100
ccttggtttg taactgtgtt actcaatctc tgcctttatc tgcacatggc cttctctcct 272160
gtgtctttct gtgtttttcc cttttttttt tttttttttt gagaccgagt cttgctctgt 272220
cgcccaggtt ggagtgcagt ggctcagtct cggctcactg caagctccgc ctcccgggtt 272280
cacgccattc tcctgcctca gcctctgagt agctgggact acaggtgcct gccactgcgc 272340
ctggctaatt ttttgtattt ttagtagaga cggggtttca ccgtggtctt gatctcctga 272400
cttggtgatt tgcctgcctc ggcctcccaa agtgctggga ttacaggcgt gagcgaccgg 272460
ttttttcctt taaggacact agtcattagg tttagagccc acccttatca agtatgatct 272520
tactttaact aattatatct gcaaagactc tattttcaag taaggtcaca tttttaggtt 272580
caggatggac atgaattttg gggggaacac ttattcagcc caccctggtg gctgtaccat 272640
gtcgcattcc ctctagcaat gcctgagagt tatagttgtt cccaatcctc acaacttcag 272700
gcttagcaac tttaggagat agaatagttt catgagaagt ctcaggactg tctctcattg 272760
ggctgccttg gcatacatgc gtgttcatcc ttgaacttat cgctacagcc cagacatgga 272820
acatgatggt tggccaggat gtggtcatat ccctactcct aaaggtaggg atggggaggg 272880
gtcagtgcca catgaatcac gtaaattgat tgtggataag gggcttcctg gaagaaaact 272940
agggagatgt taacagaaga agaggaactg aaagattgtc aggtaaaaac aactaatttc 273000
ctctacaaat aggaaagtgc cttttgcaac cgctccttat cagtttccaa caactgctgg 273060
aatcaaagca attgattgtt ttctatgtgc caggcactat gccacgtgtt ttacatggct 273120
ccaagttccc cagcattcct ctggggaaga ttccattact ctcacctttt ttttttcttt 273180
tttttttttg aggtggagtc tcgctctgtc acccaggctg gagtgcagtg gcatgatttc 273240
tgctcaccgc aacctccacc tcccaggttc aagcgattct cctgcctcag cctcctgagc 273300
agctggtatt acaggcgtct gccatcatgc ccagctaact tttgtatttt tagtagagac 273360
agggtttcac catgttggcc aggctggtct tgaacgcctg acttgaagtg accgccgacc 273420
tcggcctccc aaagtgctgg gattacaggc atgatccaac gtggccggct cacccttttt 273480
acatgtagga aactaagact tggagacttt ggataacatt gcaaaagtca tgtagctatt 273540
agggggtgac cctggatcct gggccaggtc tgctaacatc agtgcccact atactgtcct 273600
tgtatttctc cccagtctcc caaccagcca gtctgtccct gtctaccttg atttgaccct 273660
ttcattgaca tgcataattt cttggagtgg taactttttt tttttttttt tttttttaac 273720
atttcagggc ttctcctagg gataacctct gcttcctgct gagttctgga tctagagcac 273780
aggcaggttt cctccccttt ctcctgtagc tactgaactc tgactttgta gtggtggagt 273840
gtctggggcc caagagagat ttctgtcctt cacctggcaa tagtagaccg ctaatttata 273900
tcagagtgtg tttcttgccc ctcccccagg ggcagacagg cactaccttg tcctcagaca 273960
aggctcaaag catgaatggg cttccctagg tatgcctgct ccaccctaaa accttggcag 274020
gtgctgtagg gctgtaccac caagggggaa gggtctccca ggcctactgt gctgtttcca 274080
gtctttctca tgcatgccga gtagaggcca gtggaaaagg atggcaagca gatggggatt 274140
tctctgtgtc cagcgtgctc agggcttcta aactgccatg ctagcttatc ctttgccggt 274200
aagaatttgt tttcttctta tccagttttc tgatgattgt ctgttcttcc cattctctgc 274260
caaaagagga aacagctggt gtgtcttgtc cctttttgga aggacttgtg agccttagga 274320
attcagttcc cttgattgct ttgtataaca gcgcaattcg ctgatggact aaaacaaaat 274380
tatggttttg tatgtcttat aggttatcca ttttttaatg tatttatttg acacattgcg 274440
gggttttttt ttgttttgtt tttgagacag agtctcactc actctgttac ccaggctggt 274500
gcacagtggc acaatcttgg ctcactgcaa ccaacctctg cctcccgggt tcaagcgatt 274560
ctccttcctc agcctcccga gtagctgtga ttataggtgc ccgccactat gcccagataa 274620
tttttgtatt tttagtagag atggggtttt gccatgttgg ccatgctggt ctcgagctcc 274680
ctcctgacct taggtgatct gcccgctttg gcctctcaaa gtgctgggat tacaggcgtg 274740
agccaccgcg cccggctggt tatccatttt tctcttgtgt atgccagcct cacagagtgt 274800
gggatatgag aaagaaagga tcctagggtc gaataaactt gagaaatttg tcatgtctta 274860
tcctcttctg gatatttaca tggcacattc atatcttaaa gaccctgaga attcctgcaa 274920
aatagagaaa tacatatata ttgagatgga gtcttgctct tgttgcccag gctggagtgc 274980
aatggcacaa tctcggctca ctgcaacctc cgcctcccag gttcaagcaa ttctcctgcc 275040
tcagcctgtc aagtagctga gattacaggc gtgcaccacc acacctggct aattttgtag 275100
ttttagtaga gacggggttt caccatgttg gtcagggttg tctcaaactc ctgaccttag 275160
gcaatccacc agcctcggcc acccaaagtg ttgagattgc aggcatgagc caccacactt 275220
ggccttgaga aataaattta actttgttta acaaagtgtc cctaaagttt gtttgatcat 275280
agaacccttt gcttgtgtag cactcattat ttcccttaga attaatattc tgtgaacacc 275340
gttcggaaaa tagaggctgg atgttgatgt tttattgtct gggaacctgg aaccaaaata 275400
gaaagccagg atccgtaaag atagtaacag aattgttgtg ggtatttcta gaaccaaaag 275460
ccatcctgga gattgggtgg aggttctggg tgataccgag gcactcagca acggccttgg 275520
tcagtagtcc gtgtttcatc ttgtatgcta taacttcaga gatggccggg tgtggtcact 275580
cactcctgta atcccagtgc tttgggaggc cgaggcgggt ggatcacttg tggtcaggag 275640
tttgagacca gcctgaccaa catagcaaaa cctcgtctct actaaaaatc caaaaattag 275700
ctgggcgtgg tggcaggcac ctgtaatccc agctacttgg gaggctgagg caggagaatt 275760
gcttgaaccc gggaggcaga agttgcagtg agcagagatt gcaccactgc actccagcct 275820
ggacaacaga gtgagattct atctcaaaac acaaacaaaa acaaaaacaa caaaagactt 275880
cagaaaggat acctggatga atgagacaca aagtcaaaat aaaatgtctg gaagtctcta 275940
gatcattcca tggactctcc tttctgacac acccatctca ctctctgaaa ccaataagaa 276000
atctcttcct ggcccctgtg tttatatggc ttgctccgat ggctggtgct caccaaccct 276060
gctgtggttg gggcagaccc tgaatatgga gctgctatga tatagtaggg ctggatgcca 276120
acctgttttg tggatcttca tcctagttgt aaatgatttc tcagcctcaa attaaaactc 276180
cttggcttct gggccctggt ctaccccagg gatttgagag tgggcatttg gtctctctgc 276240
tctgtgcccc tcagttccat caccatccct tgaagtagcc tagacacctg ttgttttcag 276300
ctccttgcac cctttttatt tttgctcatg ggacaccacc tctctcctcc atcccatatt 276360
caggtgggtc tgcccgtcca taattgctgg tagggaccaa agctggggtt caaacccaaa 276420
gggtctgatt ccaaaaccca ctttcataaa aattactcta gcttgcttct tggtaccaaa 276480
ttacattgct tttcattgca tttaattttt aatactctag atcgtgaaat acactacaga 276540
gtttctctcc agtcatttgg ataaccacat gtcagacaat atctgagtca aggtttaaaa 276600
ttacagaaaa taaaccaaag ttctcatctc acaacaggga aaagtatcat cttactagtt 276660
ttaatggtgg caaaaaatta aggttctgtt tgaaaaatta ttttttctct aagatcctta 276720
gtccttttta ttcctcaact acatcagcta aaatgtggcc atgtatgatg attatggaag 276780
tgaagagctt tttctgaagg atactaacct aattactctg gaaatatcaa ccatgaatca 276840
agaagtatat tatgacttaa ttgttcattg cacaaaattt agataatcac tcatttcttc 276900
catgccttcc ctctctccat ctagaagcgt atttaactaa gtaatcaggg gtactaacta 276960
ttcacagcct aggcaagtgt agggaaataa ccaccttaat aacacattgg cagcctcaga 277020
aatggctggc tatgaataag cacatagcac ttagtctgct ccttcttatg tgtttgccaa 277080
tacatttgca atttcatgca cagttttaaa tgttaagcat taatctgaaa tgggcactga 277140
agcatcagta aagtctacaa tttgccccct ttaatgaaaa tgctagatat agatttaatg 277200
caaaactaaa catttggctc aatatgttct actccagtaa attagctagt gtttgtcatc 277260
tttttaaaaa aacaggggct gaatgcctca tttcacatgg ttttgttagg gacagataga 277320
aagctgataa acaacccatt tgttctatga cagtagctcc tcgtcacact actgaagtta 277380
agcttcagtg ctcatttcct ttacaaatcc tgttgtgagt ttaagtggcc tgctgttgaa 277440
tatttgactg aagtgtagcc ttgacaagag tgttttccca gaggaataca gacgaagctt 277500
ttttggacag tggccctaga gcagtggatt ctgaaatgac tcagtgaacc tgaaagggaa 277560
cagagcatca gggagcccta ctgtgcgcta tttgaaactg tcagagatgt tatcccacaa 277620
cagcctgaac aggttcatgt aattgccctc tacttggcca gatgaggaaa ccgagggcta 277680
gagatgaagc tgtgtagcta ggaagtgatg aaacctggat ttgaccccac tcactatttc 277740
acactgtctg gcagctggga gctgcccttc ctatatctct accagtggtg cattatttgt 277800
cattcaagga gcttacacat gccacctcct ccaggaagtc attgctgatt gctcactatt 277860
aaccatcctt cttatgtgtt tttattgtac tttatctgtt tccaatgtag tccttaggac 277920
attctgcctt ttggtacatt aatttaatac ctatctgact ctttataaga gaaagggcta 277980
tctcttacat tgaccttgta tatactgcag gactttcact atcaaaggca ctcgataaat 278040
gttcattgag gctgggcatg gtggctcaca ctcctaatcc cagtacttac agtggccaag 278100
gtggaaggat cacttgaggc caggagtttg agaccagcct gtgaaacata gcaagatctc 278160
atctctgcaa aaaaaaattt tttttgagac agagtctctc tgtggccagg ctggagtgca 278220
gtggtgcaat ctcagctcac cgcaacctct gcctcccagg ttcaagtgat tctcctgcct 278280
cagcctccca agtagctgag actacaagct cgtgccacca cgcccggcta atttttctat 278340
ttttagtaga gacagggttt caccatgttg gccaggatgg tctcgatctc ctgacctcgt 278400
gatccacccg cctcagcctc tcaaagtgct aggcttacag gcatgagcca ctgcacccag 278460
ccaaaacttt ttttaaaaaa attagccagg cgtggtgggg ggcaccagta gtctgagcta 278520
ctcaggaggc tgaggtggga ggatcgcttg agtccaggag gttgaggctg tagtgagcta 278580
tgattgcacc actgcactcc agcctgggcg acagagtgag acctgtctcc aaaaagagtt 278640
aaaaaactgt tcattgaaat gtttataaaa taggtggttc tcaatatctc atatcctatt 278700
tccagaatta tgaagaacag tacagtcaag ggggcaggtc ttgcagtaag cctgacgggg 278760
tttgaatcct agctgtatga ccttaggaga gttgcagagc ttctctgagt ttcagattcc 278820
ttccgttata aagcaaggat aggaatagta tctactgcaa aaggttgttg cagaagtcca 278880
ataagagaag gcacacatag tgcttagaat agtgcctggt acatagaaaa tgctcactga 278940
accttggcta ttattttcca gaaatgtttg gagattttta tagcataggc aagaaacttt 279000
gtggcactgg ttgtttacca ttttaaaacg taaaaaaaaa ggaaatattt gatttgatta 279060
atagctgaac tgacaacgtg caagaaccat ttaataatga ccaatcatga aaggttatgg 279120
aaaaagaaat gatttaagaa ggccaagagc caggccaagg tattggtaag ccctgaactc 279180
atcacatgtc tggagtcgac agttcaatca gttctagttt tgaatatggg caggagcttt 279240
ttgtaaaaaa aaagttttgg ggcaatatgc atatggcttt cttttctttc tttctctttt 279300
cctgccccct gggcaatatg tgtactgctt tctgagaatg agtttccaaa taattgtgta 279360
cctactgatg caggtgtcat aatttccaaa cccatccaat caatatgtac cagacagaga 279420
tctgctcaca gttcagagag ctaatttggt aactccagtg ggccattctg ttccttgttc 279480
tggataacca actaactaac ctactgccta ttgaacacta tttgacttgg gtaagcccct 279540
caacatccct ttgagcttga atcttcttac agataaatat ggatcaaaat atggatacct 279600
ctccttcctt tccctctttg gacctcagtt tctcatctgt aaaatgacca ggtggcctct 279660
tagtccctcc tctggctaac cttccatggt tctgcctccc ggggatgctg taaagtcagt 279720
tcaatgatac tgactaaact gctgtgaact tcctgaccca gggcccagct ggacaccaac 279780
tgactaggag agttgatggc tacacaggga agaactgcct ctcctcagct gccggacagg 279840
gaaaggaggg gtggggcagc tgctgatggc tatgtcttcc cctgtgtcct ggtcacctgg 279900
cctctgcttg catgcaacaa ctctattttt gaacaggagg tgggtggcag gagggcccag 279960
aggtctgaat tttcttaaac agccaggcat ccttgggctg acacggtgtc ctagctctca 280020
tcaaaggaca tgatgaatat atataaagcg aagctacatg agcagcccac tgtgaaggga 280080
ggagaagggc aggaggtctt gaagcctaga ttctttggct ccaggtggct ggaaaagtca 280140
atagctctag acagtttccc aagccggctg cgcaccagca gcacttggaa aggtagtttg 280200
aagtacagat ttgtgggctg tagcttagaa cactctgatt cagtaggtga atggcctgag 280260
catcttattt ttagcaaact ccccaggtgg ttctcgtgca cagcgaggct tgggaatccc 280320
tttggtttag gtcatgggga gatagaagtg ataagagaag aatggctggt gaagcacatc 280380
agctccaccc atgctccagt ggggtcagta gtatgtggca tcccagctca ttccagctgt 280440
gccatggttg tgtcttaaaa tctcaaagcc tcgatgctta tttctaaaat gggaatcata 280500
ctgacaaccc catagtatca atgtgagggt gaaatggatg atgcccataa ggcacttagc 280560
agggggcctg gcacagagtg agcattgaat aaatgtcagt tattgtcatt attgttgttg 280620
ttaacattat tatggagggc caggtgcggt ggttcccact tgtaatcgca gcactttggg 280680
aggttgaggt gcaagaattg cttgaggcca ggagttcgag accagcctgg gcaacataac 280740
aagatccttt ttctacacac acacacacac acacacacac acacacacac acacacacac 280800
acacctacct gaaaaaatta gccaggtgtg gtggtgcatg cctttagtcc tagctactca 280860
gggggctgag gagagagaat cacttgaact caggagttgg aggttgcagt gagctatgat 280920
tgtgccactg cactccagcc tgggcaacag ggtgagacct catctcttaa aaaaaaaatt 280980
aaaaagaaaa gatttttaaa ttcttaagaa aaacccaaac aaaaaccatt attaactaat 281040
gaagcacttg gccattcctg gctgttgttc tcttccttat tgtagggtgg gtgtcttagt 281100
ttgggctgct agaacaaata taacagactg ggtacttaaa caacaagcat ttctttcaca 281160
cagttttgga gactggaagt ccaaggtcaa ggtaccgaca gatccagtgt ctggagcggg 281220
cccactttct ggttcataga tggctgtctt ctcactgagt gctcacatgg cagagagcag 281280
agagacacaa gaagcaacct ctcatgtctc ttcttagaaa agcatgaatc ccatttctga 281340
gggctctact ctcatgatct aatcacctcc caaagcccct gcctcctaat tccgtcacac 281400
tgggggtaaa gatttcagca tatgaattct gttcagtccg taacagtggg atagccctgg 281460
acaacatcta aggtcttgtt tgctagatga tttgctccag agcccccaag gaactgcact 281520
tgtaacacat tttacaaaga tgactcatga aagtagggct aaaaaatgtt aatattattc 281580
aatctgggtg atgagtattt aactggctat taagatattc cttcaatttc cttgatattt 281640
gaaatatttc ataacaacaa cagcaacaat aaactaagct aatattcata aatgccagct 281700
gattggttca tgttaatccc agctggtgtg accttgatca aaatgggtaa gcaggcaaaa 281760
tggccttcag gaccctccag ctgtgtgact atactcccct ctttgagacc cctgtcagtc 281820
cctcccaagt tcttcagcca cccaagacct cccttcctca aggacctgtt cttccagagc 281880
tcctgatctc atcttggagc tagtttgatt ggaggtatta acagttgatg gtggcttaat 281940
ttcgatttct gctttttctc ataaaccaag tggcatacac gtcagacccc tgcctgtgag 282000
tgatgaggac attttgatgg agggtgaaag ttgtacacta ccctcgcaaa ctactaggtg 282060
taaagctgct ccctttttgc atggaaaggt caactccatt tcaacattat aatgttcagt 282120
ttctgttcag agcaattcag aatggtgcca aaacaactgc tcaattcagg cttgtgtgtg 282180
tgtgtgtgtg tgtgtgtgtg tgtgtgtgtt taacctgtca gtttgttggt tgaaaaaaaa 282240
agtgatgagt tgcctttatg taaacaaaat agccagttaa ttgactggga ttttcacttt 282300
ttaaccagtc atcattgcaa agacaaagag aacttttgct aagttgcttt tcacaatgtc 282360
acacagtcaa acaagcacac attcaaggtg agattttcct ttagttacta tccttctctg 282420
gtgtgaatac ctaacctgat atcaaatctt tctcttcttc cttcacagtc ctccagatgt 282480
gctttgattt ccttagtgtc ttagttttct cctgaggagc cacctctctc tcttggatac 282540
ctgcagcgct gcccaaactt caatcattcc catatggctc tcctggtttt tgccatatcc 282600
atgtaccacc cttcctatca ttgaaccagc tttctttaca taaaatcact tagaaaataa 282660
gtttattttt gccaggcacg gtggctcacg cctgtaatcc cagcactttg ggaagccgag 282720
gccgttggat cacctgaagt caggagtttg agaccagact gaccaacatg gtgaagcccc 282780
atctctatta aaaatacaaa attagccggg catgatggta catgcccgta atcccagcta 282840
cttgggggac tgaggcagga gaatcgcttg aacccaggag ggagaggttg cagtgagcca 282900
agatcgcgcc attgcactcc agcctgggtg acaagagcga aattccatct tgaaaaacaa 282960
acaaaaaaaa gaaaagaaat tcatttttaa agagtactta acattattac cataaatgga 283020
aaaccagtat taccatacat agagggtaac cataaaaatt aacatagtga aaacaaaaag 283080
cagtgttcct aaattctagc tggattccct aataagaatc ttgatctaag acctgctttc 283140
tctttgttaa taaaaaaata aaggggggcc ggtcacagtg gctcacgcct gtaatcccag 283200
cactttgaga ggctgaggtg ggcgaatcac ttgatgtcag gagttccaga ccagcctggc 283260
caacatggca aaaccccatc tgtaccaaaa atacaaaaat tacctcagcg tggtggtgca 283320
tgtctgtagt ttcagctact cgggaggctg aggcagggga atcacttgaa cctgggaggc 283380
agaggttgcg gtgagccgag atcatgccac tgcactccag cctggatgac agagtgaaac 283440
tctgtctcgg gaaaaaaaaa aaaaaaaaaa aaaggagtct caagtgttaa agtttaaaaa 283500
catatagcac caaactcaga tttttcctcc ttcatttaat ccaagcaacc aaaaaagcta 283560
ataacctctt taagtaattg ttatttaatg cctacaatga tcccttatgc taaaagtagc 283620
cacatcccac tctttggagc tctggatcct tgcaaattgt cttccaatca gactacctgc 283680
ttctattctg gccttaatac cttgaatgcc caagccaccc actcccttgt tagctttgga 283740
gtcttgcatt ctttcccagc gtcttctaca agtctcttca ggcctttgta cctcacctca 283800
cttgccactc attcaggtcc acatagatgc taattttgtg agcatttttc ctaaaccctg 283860
cctcaaagac ttccaaggaa ggctgacagt gtgtcactta gcctctcaga ggctccattt 283920
cctcatctgc aaatcaacga agtctaatca ttctggcctc acagactgtt gcgaggatta 283980
aatgatccaa tggagagaaa gtgccttgca caatggccgt aatacatgct caataagcgg 284040
cagcacgttg ttagccatgg agtcatccag tctgactcca gtgtcaactt ctttcagtca 284100
gttcttgaac tacctcttag cccttgctac acacattcac tggctcttac aaatgccaat 284160
aagctgttgg tttactcaaa ctcctggtca gtgatggttg attgtctgga atgccatgtg 284220
tgtttgcttg gtgtatctca gacctggagt agctacggaa gtcaaggagt cctgcaaggg 284280
agtgtgattg gatagcatgt cctactgttc ccaccagaga gattcatgtc ttcgtggaca 284340
gcttttgtta tcctccctta gtggacgtaa gtagagggac agtttttaca agggactgtg 284400
gccatttgct ctcaagaggc tttggagtct cctcctgagt atgaggcact aagttcccat 284460
ggtaagatca gtttctttgt acaagcctct ccagcccgtc tttgccttac acactagact 284520
cttgggaccg gggctcatcc tcttataaaa ggatgccaag cagagggtca tacagtaaag 284580
ggaggtaaac attcttttaa actagtttat caattcaaca cagcacagtg gtgttgggtt 284640
accagggtca acagccagca gtcacattcc tcagctgttc gattggggca atgcattaac 284700
aaacagccag ggaaactctt tttgattaat tatgttgata agtttttttt gtagtgtgct 284760
acaagctctt ttaatttaat ctgcttggac agcagcccat tcaggtgaaa tgtcttgact 284820
tatctaaaga agcactgatg agaaggctac tgggcctggg attttactgt actatcatgt 284880
aatcattaac acatgtggct actgttttcc tgcattttga ttttcttctt gaaaggaagg 284940
acatgtctgt ggggtggggg tattagggaa gtaaggatga gacccagcag cagtgacagg 285000
aaaagaggat gctattacta gctgagcctg aaaacactat ctttattgcc aggagtaaaa 285060
gtatttgaaa gcactaataa tggcttcagt gaatactcat cttttaaaaa agagcaaagg 285120
atggcgagga catagttctc tcaaagagtg gaagtcttct agattagggt tgtccaatct 285180
tttcacttcc ctgggccatg atggaagaaa aattgtcttg ggccacacat aaaatacact 285240
cacagtaatg ataggtgatg agctaaaaaa aaaaattgca aaaaaacctc ataatgttat 285300
aagaaagttt atgaatttgc attgggctgc atttaaagcc atcctgggcc acgtgtggca 285360
catgggccgc gagttggaca agcttgttct agatcaaggt ttctcaacct tggaaatact 285420
ggcactttgg gctggataag gctctgttgt ggggtgcctg tcctgtgcac tgtaggatgt 285480
gtagcctttt cccagtaggt gccagcagca cccctcaccc ccacaattgt gataaccaaa 285540
aagatctgta gacattgcca aatgtctcct ggggcacaaa attgccccca gttgagaacc 285600
actgtcctgg atgtacagat tcaaatctta agtagcctat tatgactctc tgctgatctc 285660
ttttccatca cctcatttaa gacacaattc aacacatagc tcttccaggg ggcccatcct 285720
cactgacttc tttgtcatct gtcctcccta cattaagacc ttgggtctat actatcttct 285780
ccttccttct cttcttcctc ttctcccttc ttgttttttc atccacagaa ctctgttctg 285840
agcaactgtt caatatcaac ttgtgaatta accagggaat tatttctcct ttatgtttgt 285900
gacagacatc tatctcagag gcaacccaga caaaatttcc caaattatcc aaaaataaaa 285960
tgaatattct ctgattaaaa acagatgtct aggccgggca cagtggctca tgcctgtaat 286020
cccaacacct tgggaggctg aggggctgga tcacctgagg tcaagagttc gagactagtc 286080
tggccaacat ggtgaaaccc cgtctccact aataatacaa aaattagctg ggtgtggtgg 286140
tgggtgccta tagtcccagc tactcaggag gctaagacag gagaattgct tgaacccggg 286200
aggcggagtt tgcagtgagc tgagatagag ccactgcact gcagcctggt caacagagct 286260
agactcagtc tcaaaaacaa acaaacaaac aaacaacaga aaaaaaaaaa cacagacatt 286320
taggccaggc acagtggctt gtgcctataa tcccaacaca ttgggaggcc aaggagggag 286380
gattgcttga aacacagagt tcaagaccag cctgggcaat gtagtgagac cctgtctcta 286440
tgaaaaataa gagagagaga gggagagaga aagtaaaaaa tacatgtcta aatgggggca 286500
gtttaaattt gaggtgagaa gtggcaggaa gaagtggcag aattacagaa attaaaaata 286560
gctcacactc tgcaagcact taatccacta tatgccacat gcattatctc atttaattca 286620
cacattatcc ccattttgta gaggaggaaa tagagtttta gtgaagtgaa gtaatttgtg 286680
caagttccca gagtcgaact ggcagtgtga ggatttgaac ctgggcagct tgatcctggg 286740
gtgggtcctc agcccctgtt cacacagtta aagtcctaaa ggggtggaag gtcaccaaat 286800
aaggctgagg accaagggca ggatgacttt tcaggagctg taagaacaac ttgtctgaat 286860
tctagaggcc ccagctttca gtgtctggac ctatgcaaat ctgcaagtga atgtggtcct 286920
atttctttgt ttccatctgt cttagtgaaa ctggccctgc ttagcggtgt ggagtattgc 286980
tttcccagtt tagaaagtta atttataaag acagctgaag tgaagaatat ggttcaagag 287040
ggtgcctcag ctagcaggtg ctgggggagt ggggtggggg tggggaatat agggctggag 287100
aatcacgaaa taaattaaat ctttgacatt tgatgactgt gggagaaaaa catgtgtgga 287160
catagatcca aagccttggc agtacctatc ccttacctgt cctaagcccc gttttttgct 287220
cattttgtat ttctcatacg aaagcacaaa gttgtagttt ccaatttctt ggtggcaaaa 287280
ttgtggcaaa cattgaaaaa tatttgaaaa agatttccct ttgctcttct ccttctcctt 287340
ctccctctcc ctcttcctct ccttctccct ccccctccct tcctcctcct cctcctcctc 287400
ctcctcctcc tcctcctcct cctcctcctc ctcctcctcc ttcttcttct tcttcttctt 287460
cttcttcttc ttcttcttct tcttcttctt cttcttcttc ttcttcttct ttcttcttct 287520
tcttcttctg tgtttgtttt tttgagatgg agtctcactc tgttgccagg ctggagtgca 287580
gtggctcggc tcactgctgc aacctccgcc tctgggttca agagattctc ctgcctcagc 287640
ctcccaagta gcacagccat catgtctggc taatttttgt attcttagta gagacggagt 287700
tttgccatgt tggccaggct ggtcttgaac tcctctcctc aagtgattca cctgcctcag 287760
cctcccaaag tgctgggatt acaggcgtga gccaccatgc ctggcctcta gcctcctctt 287820
gagccacctt ttcttcatac tgtgttgcag ccatactggc ctttccacta ttcctgcctc 287880
aggacctttg cacatgatgg ttgctttaca tgggtgctga ctcctttgcc caggtaagct 287940
cctcaggtct ttgtttacat atcacttcct cagagggcct tcctgacttc cttctctaaa 288000
ctgatttctt tcttcactat tctctcagca cttctttttt tgggggaggc agggaacaca 288060
gtctcacttt gttgcccagg ctggagtgca atggtgtgac cttggctcgc tgaaacctcc 288120
aactcctggg ttcaagggat tctttgcctc agcctcccaa gtagcacacg ccatcatgcc 288180
tagctaattt ttgtattctt gtagagacgg ggtttcacca tgttggccag gctggtctca 288240
aactcccgtc ctcaagtgac ctacctgcct tagcctccca aagtgctggg attacaggcg 288300
tgagccatga tgcctggcct tctcagcact tcttttggag aattttccat aacttaatgt 288360
ttgtctccca actacactat aagttccctg agaacaggga cttagcattc catatggcaa 288420
tggcacacaa tggcctggca cacagtgttt tataaatatt tgttgaggga atacatgaag 288480
tctagactga atatttggtt gatttggaag cagttgtgtg tttcctgcct tttaacttta 288540
aaaatgtgtg aaaattttca aatgtacata aaattagaat aatacaacga gcttttatat 288600
acacatcacc tagattcacc aattgtcaag attttgccac atttacatga ttttttccct 288660
tttttaactt aaaattttcc ctagccttct aaaggcacag aaacattagc taccattcat 288720
tgtgtttact atgtgccagt aaatgtgcta catgtttctt tctttctttt tctttctttc 288780
tttctctttc tttctttctt tctctctctc tctctctctc tctttctttc tttccttctt 288840
tctttcttga gacagagttt cgctctgtca cccaggctgg tgtgcagtgg cgagatctcg 288900
gctcactgca acctctgcct gccaggttca agtgattctc ctgcctcagc ctcctgggta 288960
gctggaatta caggtgcctg ccaccatgcc tggctaattt ttttgtattt ttagtagaga 289020
cggggtttcg ccatgttggc caggctggtc tcgaactcct gacctctcgg cctaccaaag 289080
tgctgggatt actaggcatg agccaccact cctggcctaa atgtttctta cacatgttgt 289140
tatttaatct tctcaacaac tctgctgtgg gtattataac cctattttag aaaggtggac 289200
gctgatacag tgtaagtcac tttatgcagt gttcccactt tagtaggtgg aaatctggga 289260
ctccaaagtt catgttctga actatcaaac attgctcttt atagatttaa tcaaacacat 289320
ttctctccca cctgcccttt cttcttcata cctacagact tctaaatgtt tgaaacattt 289380
ttattgtgcc taacctgtag ggcaagtgct ctagtgcctg tgaaaaaaga acagcaattg 289440
gctttattat tctcctaata aaaaatcaat ttggcaataa acatttattg aacccttttt 289500
tatttatcaa gcaccatgtt aagagctggg gagacaacag tgaatgaaat aaatattgtc 289560
tctactctta tggagcacag tagagtcagg ttctccaaca gtaattcaga tagtttgtgg 289620
ttggatccac cctatataaa catcctcttg gctttccaac tgagaccata ggtttacaat 289680
gagcaaagta agatggagca gctgacgctg agggttcaaa atagtcacct ccactattta 289740
gaggtttctt ctctgacact cccctctact tttttccttg ccctctgttt cctgactacc 289800
ctggagcagt accgctttca tctctgactg gacaaaggaa gggacaagat gtgtaattca 289860
aaattactct gatagctaca caggtcttct gtgtacagtt gtgcaatttg tgcactgcac 289920
aaagacatct ggttgaagag ctggctggag ctgaaatcct gcttgtgccc catttgccaa 289980
ggggtggccc cacctggggc tctaccagcc ttgaggaagg gcctcttttt ctacctacca 290040
agattcccat ggtggacacc aggagccacg ggtccatgag ctacagaggc atcccatgaa 290100
cttgaggaag ccctcttggc caacctagaa agagaatgga aagggagatc attgaaacag 290160
ctgggtcaaa ttgaaatttt ggattatgtg tgaaactcac catttgtact ctccatgtcg 290220
cccatggact cagtggatgg aattcaaagt gccttcaggt ttgatggcag taagaatgcc 290280
agttcaacac aggaaaaagg catggtggtt aaaagcatac actttatagg gtacataatg 290340
tgacttgaga gacttcttgc cagcaagcaa tagaagagaa tggaagaatt ggtaacaatt 290400
ttcaggtggt ttatttccta gacttaattc ctagagctaa gcagatcacc tcatcataat 290460
gacgaagaga atactaacac ctgcacagcc ctctccagca taaaccaaga ggtttttcac 290520
agggagtaca ttcattgttt ttctgattgt gtatagcaat attaatagct ttcatttagt 290580
aagcacagat acgtgcagga cattgtactg agaatttcac ctacattgct ttattgaatt 290640
ctctttatat ccccataaag gaggtatgat tatgttttat gcatggggac actgacattt 290700
gagttactca aggtcatata ataaagaagt ggtacagcag ggcttgaacc cttgcctttt 290760
gacttcaaaa cccacacctg gccatgcgca gtggctcaca tctgtaacct agcactttgg 290820
gaggccaagg tgggtggatc acttgaggtc aagagttcga gaccagcctg gccaacatgg 290880
tgaaacccca tctgtactaa aaatacaaaa attagctggg catggtggca ggcacctgta 290940
atcccagcta ctcgggaggc tgaggcagga gaatcgcttg aacccaggag gccaaggttc 291000
agtgagccaa gattgcactg ctgcactcca gtctgggtgt cagagcaaga ctgtgtctca 291060
aaaaaaaaag aaaaaagaaa aaagaaaaaa agaacaaaac ccacacctgg aaccaccacg 291120
catgcctgtt ttgggggaac agtctgttct gttaggtctc tatcctggcc tgggggacct 291180
accacacaca tctcagactt ctgttcaaac atatccaact tcctccaagt tgggaccttt 291240
tttagctttc ttttcttgaa tacccactgc cttcatgcat aactgatgaa taggaatcct 291300
tcttgggcag tgcagctaac tggccacttt ttctcagtat tatcacaatt cacactcatg 291360
tcacaagcca caccccagcc aaggtcttta acctctgcca acctcatggc tcagtagaag 291420
cactgctata agtatattaa aaaactttgt aagggttttt ctgccaacct caatagctca 291480
gcagaaaact gacataagaa aatattttaa aaaccttata aggatttttc tctttgacaa 291540
tctccccaca gctgtccata ggagaggaca gtaatcgaaa agacgtgtct ttttgattaa 291600
tgggatttgg ggggatcaaa gagacttcag tttaacagga tgaaaaatgt ctttcgtggg 291660
aagttggggc aggaggtgtt ggcactgaga gatggttttt atggtcctaa gatcacaggt 291720
atgatcaggt gttgacgata attatttttt gttttgggtt caagtgagag aattggtacg 291780
caattcttgg ctagcatggt attttctttc accttttaaa ttgaagagtt tatagttttt 291840
gtaagacaaa gttaccaacc tcatgataat aataactcac atttgttttg tacttacctt 291900
ataatgcttt aagtagttta taaacattct tttatttaat cctcacaacc actttgtgaa 291960
ggaggcatat tcattattgg cattttacag atgacaagat ggagtctcac aaagggtatg 292020
aagttgtgac tgtgtaagct gaacaagtgt agagagctaa tgtacaatat gaaaactaca 292080
gttaataata ccgtattcta tactggtgtt tgccaaaaga gaagatttta ggtacttttt 292140
tttttttttt tttttttttt ttagacaggg tcttcctctg tcactcaggc tggagtgcag 292200
tggtgaaatc tcagctcacc gcagccaaga cttcctgggc ttaaatgagc ctcccacctc 292260
gacctcctaa agtgctgggt ttacagacat gagccaccat gcctggcaga ttttgggtac 292320
tcttaataca cacacaaaca cacacacaca cacacacaca cacagagaaa gagagagaga 292380
gagagagaga gagagagatc tgggtgcagt ggtacacgcc tgtagtccca gcttcccagc 292440
tacttgggag gctgaggtgg gagaattgct tgagcccagg agttcaaggt accactgcac 292500
tccagcctgg gtgacagagt gagaccctgt cttaaaaata ataataataa taaaataaaa 292560
taaagaaaag taaaaaatgt agctgtgagg tgatgggcat gttaatttgc ttgactatgg 292620
taatcacctt ggcatgtata tgtacctcaa gataagtgta gcaaaacgtg ttacacatct 292680
taaatatgta caatttaaaa acaagacaga ggctcaggaa ggagaaataa ctcgctcaag 292740
gtcacatagc tagtaagtgg cagagcctga gttcaaaact gagcagctgc ctccaggttg 292800
ggcttgtcac cactatttca tgtaccctct aaattcttgt ggggaaatca gggtgcttat 292860
tcaatccatg ctgcatatag aggttgttag atggcgaata cttcttgtcc attttttgtt 292920
tcaaagatgg ggtttccccc aaatacccct ggcctctgtc tcacctttac tcaccctctg 292980
gggagatgag cgagttgctt gagtgcgaga agaaagggca ggcatggcct agtcttttaa 293040
ctacacatct tgttctgctc ctccaggggc aggtcttcct agttgggtga ggttggggga 293100
ggccttcaga gttctagtgc tccagagtat gtgagtctgc ctatctttgg agtttaatgt 293160
gaggagaccc ttgaccctat ataagccaca gtttccaaat cagcttatta aaggggatat 293220
ttgtccccta tttgattatt ctcttgtctt ctttctctgt tgatctagaa ctcagatgga 293280
gaagagagtg ttatgtatcc tcactgcacc ctctcccaag agctattgtg gaaaataaag 293340
aaaaaaatgt gggtcgggtg cagtggctca cgcctgtaat cccagcactt tgggaggccg 293400
aggcaggcgg atcacctgag gtcagaagtt cgagaccagc ctggccaaca tggtgaaacc 293460
ccgtctccac taaaaataca aaaattagtc aagcgtggtg ataggcgcct gtaatcccag 293520
ctactcggga ggctgaggca gagagttgct tgaacctggg aggtggaggt tgcagtgagc 293580
caagattgcg tcactgcact ccagcctggg tgacagagcg agactctgtc ttaaaataaa 293640
aaaaaataaa aaagaaacaa agaaataaag aaatgtagtt aagtatcaag acatcatatg 293700
aattaaagac tcgatctaac atccaatgaa ttggctgggc acactggctc atgcctgtaa 293760
tctcaatact ttgggaggtt gggcgggtag atggcttgag cccgggagtt caagaccagc 293820
ctgcacaaca tagagtgacc ctgttctacc aaaaaacaca atatattagc cgggtatgtg 293880
gcatgcacct gtagtctcag ctactcaaga ggctgaggag gcaggtttgc ttgagcctgg 293940
gatgtcgagg ctgcagtgag ccaagatcac accattgcac ttcagcctgg gctacagagc 294000
aagaccctat ctcaaaacaa aaacaaaaca aaacaaaaaa acaaaaaaca aaaaaaacca 294060
acaacaaaaa aaacccatcc aatgaatcca ctgccttcct tggcaattgg ttcatcaatt 294120
gagttaaaga aggagaatat agcaggtact tggcttacat gatctcactt aactgctgta 294180
acaatcaggt gagatgagat ttattatctc tgtctcatcc ataaggaaac tgtatcttat 294240
tcagtcaaca aatattgggt atagcccata gccagacttt gttccaggag gctcaggaag 294300
gtcatgggaa ttgctcgaga ttgtatatgg ctggtcagtg gaagaactag gatttgaact 294360
caggtctgac caatgacatg ggttaggttt tccttggaga gatgggagag tgggaacaaa 294420
taaaataatg acaataaaca tattgagccc tgactttata ccagtatgaa actttatacc 294480
actcagtgct tgtgaagcat tacctcatcg agtctttgca tcatccttac aaagcaggta 294540
ttgttatcct cattatacac gtgaggaacc tgaggctcaa tgatgttaaa cagtaaatgg 294600
gccggggcgg tggctcacgc ctataatccc agcactttga gaggccacgg tgggtggatc 294660
acttgaactc aggagttcga gaccaccctg gccaacatag tgaaaccctg tatctaccaa 294720
aaatacaaaa attagccagg tgtggtggtg catgactata gtcccagcta ctcaggaggc 294780
tgaggtggga ggattgcttg agactggaag ctggaggttg caatgagctg agattgcacc 294840
actgtactcc agcctgggtg acagagtgag atcccatctt tttttttttt tttttttttt 294900
ttttgagaca gagtctctct ctgtcaccca gactggagtg cagtggcatg atctcagctt 294960
actacaacct acaacctcca cctcccaggt tcaagcaatt cttctgcttc agcctcctga 295020
gtagctggga ttacaggtgc ctaccaccat gcctggctaa tttttgtctt tttttcagta 295080
gagacgaggt ttcaccatat tggcagattg gtctcaaact cctgacctca agtgatccgc 295140
ccacctcagc ctcccaaagt gctgggatta taggcgtgag ccaccgcccc tggcccattt 295200
actgtttaac atctgggatc acaggtgtga gccaccatgc ctggtcaacc ccgtcttaaa 295260
aaaaaatcaa aacaaaacag taaattgcaa tccaggtctt ctcattccaa accattagcc 295320
gatgtgtagt attgcctttg ttttcctgcc tttacaaagg tagttgggag acctagcctc 295380
tgacatgaca tcagaacacc ttgaaacaat caattaaaaa gtgcagatca atctggtggg 295440
tgtgcatgga ggtgcccaag agtggggaag gaaggcagag gatgatgtat gtgggaaaaa 295500
gggctgggga gcagagtgaa gtaatgaata ttgggtaggt tgaaccaggc ttcatgggag 295560
gcattttcag ccaggtttca gctgatatat gcttgatgac agcagagaaa aataacaggg 295620
taagtttgag aatcagggat aatttttgaa tggcatgact ttgtacatgg taagtcagtt 295680
gtaaaaaaac aaacaaagca acagcagcaa cagcaaaact ccatctcctc tgaaacggag 295740
gaattgccct gttgaagaga tgggctgcat ccaggtcctc agggttttgt ttgggtgaat 295800
attcaaggcc tgaaagaccc tggatcttgt catggctttc tgttgttaat taactggggt 295860
gactatgggg tcatttaata tccttatctc taaaatgaat gttgagtcct ccaaagttcc 295920
atctagtgat aatatttatt ctcctttctt gcaaggcaga gagaggacag taccagcctc 295980
tggcacacag atgatggctg agaaatgctt gttggctggg tgtggtggtg caaacctgta 296040
atcccagcac tttgggaggt caaggtaggc aagttgcttg agtccaggag ttcgagacca 296100
gcctgggcaa catggcaaac cccatctcta caaaaataaa caaaaaatta gctgggcatg 296160
ttggtgcaca cctggggtcc tagctgctca ggaggctcag gtgggagcat cgcttgagcc 296220
caggaggtcg aggctgcagt gagctgtgat tgctgcactc caacctggga gacagagtga 296280
gactatgtct caaaaaacaa aaacaaaaac aaacaaacaa aaagcttgtt gacttgaact 296340
aactaaaggc agtagtacta attgaaaaag aaattctcac catgtgggca tgtcctgctt 296400
gctatcttgt tgcctgtgct ccataggtca tgtggccttg accctgagtt atccagcttg 296460
tcatctcctt gttgaagaaa actctccccg ccattcccca cacaaatcac tttgcttccc 296520
tcattctaaa ctctctctaa tataggctca atctctctat gaatcccttg cattcttaat 296580
tcttccttcg ccgtctgact ttcctgttct taggtatggc tgaactggcg gtgtgcttat 296640
ttttcatttt tcatttgatg aatggataga gcatctcact tgctctcttc ctagagactg 296700
aacacatgcc tgcatgaagg gtatggagga agccgtgtga ctgagggggc aatgtctagt 296760
aatgtcatgc cttaattgta ggctcaaatt ttaatgatga attaacttct tgaaagttga 296820
tctttaggcc aggtacagtg gctcatgccc gtaatcccag cattttggga ggttgaggca 296880
ggaggatctc ttgagcccag gaatctgaga ccagccttgt gacatggcac aactccatct 296940
ctactaaaaa tacacacaca aaaaaaatta gcttggcata gtggcgcaca cctgtaatcc 297000
cagctactcg ggaggctgag gtggggagat cacgtgaacc tgggaggttg aggctgcatt 297060
gagctgtgat catgccactg cactccagtc tgggtgaaaa agcaagactc tgtctcaaaa 297120
aaaaaaaaaa agtagatctt taaaatgaag tgtacagtga gaagaatatt tcaaggagga 297180
aggatgcttt catatattgg gtttaag 297207
<210>3
<211>125421
<212>DNA
<213>Homo sapiens
<400>3
caacaaatta ctacaacttt aggggttgaa aacaacgcaa gttcattgtc ttacagttct 60
ggaggtcaga agtcttatta agtgggtctt acggactaac atcaagatat ctgcagagct 120
gcgtgtcttc tggaggctaa atgggagaat ctgttgtctt aactcttcca aattctggag 180
atcacatgca ttctctggtt tgtgatccct ttctccattt gcaaaacaca acacttcaat 240
ctctacttct gtcatgacat cattcctttt tattctgaat tactgcctac cgcttataaa 300
gactcatgat taattgggac cagttggata atccaggata atttccccat cgcaaaatcc 360
tagtttaatc ccttttgcaa agcccgtttt ataatgtaat gtgatgtatt cacaggtttc 420
agggaaaagg acatgacatc tttgggaatc attattcagc ctgctccaca ggatgcaatc 480
tggccccaaa agattcacgt tcaccccaca taaaaataca cttgctcctt cccaaggtac 540
cccaaagtct caatgcatta caccatcaac tcaaatccaa aatctttatc taagtctttt 600
caggtcaaat tctcaaagtc gctgcagttc caaatctcat catctacatc acctaaatta 660
ggtttggatg aggttctggg tataaagcat aatggggaaa aatttatctc catctgtaga 720
cttgtgaaac taaaaaacaa gttatctgct cccaaaaatg caatggtgga gcaggcatag 780
gataacagtt atggacattt ctgatccaaa ggagataaaa tagaaggaaa aaagaagtca 840
catgttccaa gcaattttaa aatccagctg ggcaaatacc acaagaatcc agtttctgga 900
aataaacctc catagcttga gtatttgcct tttgggcctg tggctctgcc cttggtgtta 960
tccttgcttt ttttttgaag ggtatttgcg gctgagtagt tttatcagcc tgtttcctgc 1020
caatagccag ggaccgggga ggtggaaggt gggggtgggg gtggaggtgt ggagggtggg 1080
ggtgtagggt gggggctgga gggtgggggg gtggaggggt ggaggatggg agggtggggg 1140
ttggaaggtg gggggtggtg ggagtggagt gtgggggctg gagggtgggg atggtggggt 1200
ggagggtggg gggtggaggg gtggagggtg gagggtgggg gtggtgggag tggagggtgg 1260
gggctggagg gtggggatgg tggggtggag ggttgggggt ggaggggtgg agggtggagg 1320
gtggtgggag tggagggtgg gggctggagg gtgggggggg tggaaggtgg gggcggaggg 1380
gtggaggatg gggggaagtg gggtggttcc gcagccttct ttcatttcat cttcactttg 1440
tccctttctg tacaacctgc agtgtttgcg ttgttataac attatcaaaa gctttatgga 1500
tctcccattt atatctggag attcacttca ttagacacaa ggcttcttca ccaatgtttt 1560
ctggataatc acatctccat tcttggcttc tgctgagatg gctggtggaa ttcattatcc 1620
acacgcctaa tctcttcaaa gatcctttta tttgactgaa tactctttca gatccttgtc 1680
aggcactggc aaagtgttat ccagctcgac ccttgagttt ctcttcagag ctcatgttcc 1740
tggcaataaa actaatttta ggatattttg aagtctgtag aggctgataa tttctcaaat 1800
cataaagtcc tggttcctgt ttgcttaaca gacttttcct taacttatgc tttcctctca 1860
tcctttatca taaacagtga gaaataattg ggttgcccct tctacacagc ttggaaatct 1920
cttcaggtaa atatccaaag tcatcattta caagtgttgc tttccaccta acctcatgat 1980
aaaatttggc tgcatttcct gtcactacgt agcaaggatg ccttttcctc cacttcacaa 2040
tacatagttt ccgtcgcctt ctgagccctc actagtagtg ttttaacact catctttcta 2100
ctaaaactct atttgtgatg atttagttac tttctaagat gatacagctt tactatactc 2160
ctcacttctg tctgtgtctt caccgtcagc aatttaacga ccctgtttac aatgatctag 2220
acattcttta agataataca agtttttttc ctactatact cctcattatt cttccagcct 2280
ctgcccctta cccatttcca aagccagttc tacatttttg gcatttattt catatagcaa 2340
ctctccattt ctagatacca aaatcgattt accatttttt gaatgtctcc cattttccag 2400
aattaagcta agtgctttat aaacatctta attatcttaa caactaccca aggggcacat 2460
tattatatat attttacaga tctgaaagac agttcagtat gatttaaaaa tgtcagtctt 2520
tggccccagg cagatgttga tttaaattct gatttcataa cactttagct acatattctt 2580
gggaaaatca cttagtcttt taaaattttc tgagcttcag ttgcctaaaa actaaaatag 2640
tagaaataaa ccctactata gaaaacaaat tttattgcct tttcttttat acattttttt 2700
tctttttctt ttttgttgtt gttgaggggt acatagatat gcagtctgct tgccttaatg 2760
ggcatgcaat aggtgttatt cttgatgttg ttctattctg ttgtcaccta tggaattaat 2820
tcatatgaaa aagaatatta ccagaggaga ggaaatatac ataataaaga tgcctggtaa 2880
aggagaataa aataggtata tttggagaga cttaggctaa tgtagctgat gaaagtttct 2940
aaaagttctg tggtgactga aatgcttact ggctccctca actttccatt gctttccttg 3000
aggataatta atagatatca gatatatcat gaattaatta gggaaaagat ggtagagtca 3060
gcaaagtttt tgtttgatga aaattcagat gtggaggagc tctgtatttc tttctttctt 3120
ttcttttttt ttttaaacag agtttcactg tgtcgcccag gctagagtgc agtggtgtga 3180
tcttggctca ctgcaagctc cacctcccgg gttcacgcca ttctcctgcc tcagcctccc 3240
aagtagctgg gactacaggt gcccgccacc atgcccgtct aattttttgt atttttagta 3300
gagacggggt ttcaccgtgt tagtcaggat ggtctcgatc tcctgacctc gtgatccacc 3360
caccttggcc tcctaaagtg ctgggattac aggcatgagc caccgcgcct ggctggagct 3420
ttgtatttct aaggaaattt agtgataagt cctgaaatca tcttgaacaa tgaaaaaggg 3480
ttattataat cggtagttac ttctaatttg tgttatatcc ttctaaagta gtttacttaa 3540
taaaaagttc catatttggt ttgtcaacct tggggattta acacttcaat taaaatgtga 3600
tacttaacat aaaaagagaa aaaagaaaga gagggaacct ctgggtctat atttattact 3660
tgcctcctca ataaagaaag aattgtttaa aacagtttat tttaacataa accaacccag 3720
gcgtcatctc agagctatct ctttggaatt tgtctgcaaa tagaagtttt atcatttgtc 3780
ttttgaaaag ggcacccaag taggaagaac agctgtagtc tacaattttc aaacttcata 3840
acgtaatttg ttgactactt ttttcaatta agttttatgc tgtcagatag tgaggtggtt 3900
aatttatata tgtttgcata aatatatact gtgctggaaa ccccaaattg tcatcatttt 3960
tataggttta aaataagatt tactgatgaa actgtttcct ttctttttca tgggaaagga 4020
ggaatcatgt gaagaatttg gacataaaat agaccaaaat tcaaattcca gttctatcac 4080
gtactctctt gtacctcgga tcttaacttt gttgagcttc ttactcatct atgaaatggg 4140
tgtaataata catttaaatg agataacata cagctcctgg caaataatat tttctcagta 4200
aacatttttg ttattcttta ttttcatagt ctctgttaca agtcaggtaa attatctcta 4260
gtcctaccca attgccaaac cagtaaatat aagttactgg ggcagttcag aattttcaat 4320
catcctatgg ccttacctcc aatttttctc tacacatata tttcctttat accactaagc 4380
aaaattagaa atgtcttggt ttatatattt cttcccatgc tttctgtctc tacaaccaga 4440
atataagctt cagaacaaga gtattcaaat ctggtgtgtt tatccccaga ctcacaacac 4500
tgggtccaga gcaggtcctc agtagatgtt tataaatatc aggatgtatt acatatatta 4560
actttttatg agtagttatt atttattata ttccacttag atatggaatt attacttcag 4620
gtggtagctg acttgtactg gaaaagtgac tgagcccaac tctaatgcta atgttaattg 4680
gatttatatg aagtttcttt ggttttaatt gcactttgaa tgaattcaag aatttcacta 4740
gctataactt ctatctatgg taagaagcgt tgtccaagcc acagaacctc aagattacaa 4800
cagagattta aattaaaact cttctaattg ttattattgt aattattata attcaccaac 4860
tggacaattg gaacaataaa ctcttggaat ttaaattaag tatggaatgg ggatggatgt 4920
ttggggtgct ttatagtttc ctgtctccat ctctctcagt ggcctcttac tctcaaatag 4980
cagtggtaat tcttcttgga tcattaacct aaaagagtct caggaatgct ttgtgaccat 5040
tgctaccgaa atataactac tcaatatgag tgggagataa tgggtctgga tatgttggcc 5100
acatacatac caggtggaag ctaggcctgg gtaggaaccc tgaaaaacgc tttgcaagaa 5160
aattcaatat ctttgaatta caataacttt caggaattct cattccattt agaatgtaga 5220
caaaggctaa taaccaaact gaggtctctc cctgaccatt gaaggctggt tctgggttct 5280
gcagggtgcc ctgccttgca aagctctgat gtgctctggt ttcccacatt ttcccacatc 5340
agcgctctgt ttgcccacat tttcctttct gtataactgg atgaagaccc cattactgag 5400
ttttaatcag aacactacct ctacatgcct ggctagggta aaagctattt ctcatagtca 5460
gttcttccaa ttccagacat agaaaaatca caacattcta ttttcccaga aggaatgaag 5520
tccatctcag ccaactgctc ttgacatctg tctccatctg tattagtctg ttttcacact 5580
gccataaaga acttccctga gactgggtaa tttataaaga aagagattta attgactcac 5640
agttccgcat ggctagggag gcctcaggaa acttacaatc atggcagaag ggaaggcagg 5700
cacatcttac ttggtagcag gccagagaga gaaagagtga gtgaagggca aagaatccct 5760
tataaaacca tcagatcttg tgagaactta ctcactatca tgagaagagc atgggggaaa 5820
ccactcccat gatccagtta cctcccacca ggttcctccc ttgacatgta gggattacaa 5880
tttgagatga gatttgggtg gggacacaga gccaaactat atcactattt tttgggtacc 5940
ttccatttct ttcagcaaag ttagattttt tttggggggc tttaacatag ggttcctttt 6000
tggatgttcc accaaattac tatgacatta cagtaatata aactgagaaa acagatcagc 6060
aatagacaga agagtttgtt atgttttgtc tttttttttt ttttgaggtg gggtctcact 6120
ctgtcaccca ggctagagtg cagtggtgtg atcgcagctc tctgcaacct ctgcctccca 6180
ggctcaagtg atcctcccac ctcagcctct tgagtagctg ggaccacagg catgtggcac 6240
catgcctgac taatttttgt ggttttggta gagatggggt tttgccttgt tgcccaggtt 6300
ggccttgaac tcctgagctc aagcaatcca tttgccttga cctcccaaag tgctaggttt 6360
atttattttt taagacattt taaattaatt tcagtcaaat acattttaga agaagatctt 6420
atactgagaa ggtatcccca ggtctttttt atccaaaatt gcataaaacc tcatttcttt 6480
tcactgcaca attattttca gtgtgcataa tattcacccc taaaaaaatt ataatgatat 6540
atgtaatatt ggtatagaat gctgtatatt caacttaaga aaacgtacaa ttgcatttgt 6600
gaactaaata acacgttact aacagtttat tttcactttt tttttttttt tttttttttt 6660
gagatggagt ttcaccttgt tgcccaggct ggagtgcaat ggtgtgatct cggctcaccg 6720
caacctctgc ctcccgggtt caagtgattc tcctgcctca acctcccagg tagctgggat 6780
tacaggcatg caccaccacg cctggcaaat tttgtatttt tagtagagac agggtttccc 6840
tatgttggtc aggctggtct caaactcctg acctcagttg atccgcttgc cttggcctcc 6900
caaagtcctg ggattacagg attgagccac cacacccgac ctattttcac ttatttttgt 6960
ctttgtcttg tgggttgact tgtcctgcat ctatttagcc cagtaaatgt tgctgttcta 7020
cttttcctgg cttcataatc cacttttagg ttctagctgc atctcaaata aatattggca 7080
aagcaattca aatccttaaa atgcctcagc agattgatgc attctaagct ggagaaaaat 7140
aaaaacagaa tcatacatca tgtttatcag cctttgccat agagttcttt gactataatg 7200
aattcattga gtcaaacaac tttttccatg tctctctctc actctgaaat ctggctaaat 7260
aaacgtgatg tttaatatct tgcaagtata tttgcatgtg atggtagttt cctgtgtaat 7320
taaaaaaatg gggaaaatga gcttatatat tggctctgat agaacatatt atggtggtaa 7380
atttgaagga agctgaaggg caaacatagc tgttgacttg gcaagttgtc atgactaaga 7440
aggtattctg ggacctatac tcagtaaaaa aacaataagc cactctcagc ctagattttc 7500
ttctttggag gacttggccg ccagtgtcat ttgcaaccaa gaaaactttt ataggtctgt 7560
tcttatcaga gccttccttt catgaacagg caggtcagtc aactcatctt cttttctcaa 7620
caatttagtg aagtcagaaa gactgaaaga caaagcaaaa agaaagccag agttacagtg 7680
ttttagataa gctaaagcat ttattgtttc accatagcgg tggctgatgc agatatatgt 7740
agctaatatc tttaaaattc tggttgattc cctttctggt tggttgcata cctttcccag 7800
tgtaacttct cagtgggagt aaatgatgtt catctagacc aaagatgcaa gctatcattt 7860
ctgtttcctt cccacatagg tatttgtctt tgttttgcaa gattttcttt ctctatccct 7920
caatttaaaa ggcaaattcc ttaaaaatcc atggacaatt tcaggtgtat agtgcatggt 7980
tcaattgccc cagaggataa tgttgaagtt gctttgcaga ggggtgcaag agaaaggggg 8040
gtgtaatcca agataatagt aaaagccgat aagcattgcc atttcaggaa actctgccac 8100
ataacttggc agtttgaaaa tttagccaac ctttttatag ggaggtcatt aaattccagt 8160
atttcataca tattgtgctt caaagcaggc aggtattcat tatttttact tcattttgtt 8220
ttattaaaat aaatgcttca gaatacctta tgcaaaacaa aatccaaata catttttgaa 8280
aacacatgta tactttgctt ctaaactaga aaaaaccata ttgctggttt aggtgacaat 8340
tcaataggaa gtgaaatttt ttaatctaat acatttttaa aaagctgaga gtttactagt 8400
ttcatatgta ttttgccact aaatccctct tcattaagga tatttattgc aaaataatca 8460
tctttagaaa aaggaggtag atgtcttgat tttgaaactg aacgataaca tgaaactgaa 8520
taacatgcat aacctttaca tttagaaaca aattgctata attgcctctt gaacaacaaa 8580
taagcaaaaa taacattaaa attaaaatat tgtaatattt cttttaaaat tattttcctt 8640
tttttgagac agtctcactc tgtcacccag gctagagtgc agtggcgtgg ttatggctca 8700
ctgcagcctg gacctcctgg gctcaagtga tcctcctgcc tcagcctccc aaaatgttga 8760
gattagaggc atgagccacc gtgcctggcc ttgtaatatt tttcaaaaaa ccgcttctta 8820
aaccaggaag agaaaaagat atgcatcata attcagaatg cagtgtgtca aatgacttaa 8880
ttttatttga tctgcagcag aaaaatgtaa tttcttattt gagattcttt gatgttgcca 8940
tttcactttt gcctttcttg ttggctaata gctgggaatt atttcctgga aaagtatctg 9000
atccattaag gaaagaatat gaccatgttt cgtggaaatt aaaaagtaat gtagacaaat 9060
attcatagaa gatacaagtg tgaccctaaa gttagtattt gaatgttgtt ctcctatttc 9120
tgattttatt gacccaaaat atcaagctag gtttatccat gtttgcgtgt agcattttaa 9180
ttacttagcc taagaaaaga tggaattgaa tttcaaatgt aaagcagtag gaaaaaaaaa 9240
gagagatttc tctttaggtt ttcaaaaatg tatttcttgg agcttcttga ttttggcaga 9300
atgagcaaaa acaggactga gaggactgaa ctaactattc ttgtcgttga atgtgggagt 9360
tatttctgtt tgcttcctct acaaaatcag tttctccaac cacatcttga gaagttttcc 9420
tttgcacaac atgtttgaca aattgccaag caaaggcatt gaagaatctc ggcttcatcc 9480
tacaaccttt ttcttcatct cagaaccttc aacctacaac tttttcttca tctcagaaca 9540
cgtcttagaa gatatgagtc tccaaaatag cagaggggaa tgttttactt aaaaaatgtg 9600
tgtgagatca ctgtacataa agttattcac atgataatgg atacagaact ccattgatga 9660
aaaacgttag gaattttctc atatatttaa atgtttgata agttattttc tttattttgg 9720
tgaccagaat gcacacaaat ggttgcataa tttatgtaga tatatgcaaa agctctgcca 9780
acaggtactt gtgggagaat caaagtctgc tctatccatg cattcctttt acattccttt 9840
tacttccacc tccacttctt atttctttct tttttttttt tttttttttt ttggagacgg 9900
agtctcactc tgttacccag gctggagtgc agtggcacaa tcttggctca ctgcaacctc 9960
cacctctcca cctcccgggt tcaagcaatt ctcctgcctc cgcctcccga gtagctggga 10020
cgacaggccc acgctgccac acccggctaa ttttttgtat tttagtagag atgggttttc 10080
accgtgttgc ccaggctggt ctcccaaagt gctaggatta tgggcatgag ccactgtgcc 10140
cggcctcacc tccacttctt aacaagtact ctatgctcta agttcattat tttctcccac 10200
tgggctccct ttgctattgt acttctactc agttattgct cccgttttta ttatctgcct 10260
ccttattctt cttgtaaccg aagcattctc tctgtaggtc caaaataaag tccaaggact 10320
agcagacact ttccttttca ggacacaata tgttatctca tccctggaat acctaggata 10380
catcgcacac tccgtgcacg gtgtgtgtgt gtgtgtgtgt gtgtgtctgt ggtctgcgtg 10440
tgtatgcttc tttgttatgc attgttaatt tctttcacta caagtatttt aagttttttg 10500
aacaaaaaga actatatttc aataatagtt atcatctact gaacatctga gagtagcaac 10560
aaatgttaga gtgaacattg taaaataatt aaaaggacca aattacactt tcttacctgc 10620
cttttttttt tttttttttt tgagatggag tcactctgtt gcccaggctg gagtacagtg 10680
gcgtgatatc ggctcactgc agcctctgcc tcctgggttc aagcaattct cttgcctcag 10740
cctcccaagt agctgggatt acagaccacg caccaccaca cctggctaat tttttttttt 10800
gtatttttag tagagacagg gttttgccat tttttctgta atattgagta gacatctggc 10860
ccattcacat acctaatagt aaatgttata atgtaaaaaa aaaaaaaaaa aaatactagt 10920
ttctacaaat attgtctctt ttacttttat agccagagaa tcactttctt atagttagat 10980
atggtattgt aactagttct ggctaatgga atgtaagcaa agtgttatgt gcaacatttt 11040
ttcaaaggtt tagggcaaga tcttcatcct ttatggctgg ggtacaatca atcatttcgg 11100
accagaaatg aacatgggat ctgcatggct tcatattatc agccatggac aatatcattt 11160
gagaaaagga ttgttaaaaa ttaatcatag aaacaaacaa aattttattc tattttgttt 11220
aagccactgt tagtttgggt ttctgagatt cactgctgaa tgttattctg atacctccac 11280
ttcaacctac gatgtgtaat tcccagttta cctgggacag tcctgaatta cagtgatgtc 11340
ttgtaatttt tctgctactt cccaggaccc tcaaaagtac ctggcttagg tgatatgtta 11400
tatagtgatg cttcttaatt ctctagactc cgaatgttat tagctacagc aacatttatc 11460
gaagtaacat ttttcctagg agtttaaatg taaaacagct aaagacaaag ttactctggg 11520
tcagacatgt ctggaagagc tacagttctc cctaatacct acagaccaca aactgggcct 11580
gaactggtat ctatatatgt gtgtgttggt gggggtgata tctatttatt catttgttta 11640
ttttttttcc actggatagg aatttctaac aattaaaata tgttattgtt cttagagcta 11700
aacataatga taaatgtaag ggagacataa ggaagatcct acatatagtt tttgtttgtt 11760
tgtttgtttg tttgagacag agtctcactc tgttgtgcag gctggagtgc aatggtgtga 11820
ttttggctca ctgcagcctc tgcctcccag gctcaagcaa ttctcctgtc tcagtctcct 11880
gaatagctgg gattacaggt gcgcactgcc ctgcccgaat aattttttgt atttttagta 11940
gagccagggg cttcaccgtg ttccccaggc tggtcttgaa ctcctgagct caggcaatcc 12000
acccgccttg atctccaaaa gtgctaggag tacaagcatg agccaccgca cctggcccct 12060
ggtgtatagt tttagagact ccttttcaat tgtgtcagaa gtgtttgaac cagagcaact 12120
ccatcttgag taggggctag gtaaaatgag gcagaaatct actaggctgc attcccagat 12180
gcttaggcat tctaaatgat ggatgggata ggagatcggc acaagataca gttcataaag 12240
accttgctga taaaacaggt tgcaattaag aacctggcta aaacccacca aaaccaaaat 12300
ggtcacgaga gtgacctctg gtcatcctca ctgctacact cccaccagtg ctgtgacagt 12360
ttacacatgc caaggcaaca tcaggaagtt accctatatg gtctaaaaag gggaggcatg 12420
aatactccac cccttgttta gcatgtcatc aaggaataac cataaaaata agaaaccagc 12480
agcccttggg tctgctgtgt ctatggagta gccattcttt tattccttta ctttcttaat 12540
aaacttgcgt tcactttact ctctggattc tccctgaatt cgtctttctt gagcgaaagc 12600
caagaccctc tcttgggatc tggattggga cccctttcct gtaacaattg tgtcctatta 12660
cattcagcca atttagcaaa ttgatttatg attcttacat atccatagga taagtatgtg 12720
gattatcttc attttgttac tgatgagaaa atggacactt aaagaaattc agtgactttc 12780
cagggttaca taccatctga ctaaatggca aaactgaaat caagcccaaa tacactctac 12840
ctcctctcac aaattttgga aacatttaat ccaatcctta aatcaacaga ggatcaaact 12900
gaggcgcagg gattgctgaa atagacaagg agtaacaaag tttgccccgt tagagccaaa 12960
tgatgagctt ctggcctttt gaaaagttta gttgtaaaat agtccctatg agtaggaagg 13020
aagacacatt tttatttagt tttatgtaaa tggtgcgaga caaattcagc cttgcctaag 13080
gcaactgaaa aaccatcttt gagtgaatta ttcctttgaa gctttatctt caaaaggcac 13140
tgctactctt taactttggc aggaaagggg aacacctgca gcaccgttgc ataaattggc 13200
ttcgttggac ctccttcatc agccagagct ggagacctac gtgggttata atgtctggaa 13260
agggcaaggt ggtggcctga gtagtctgaa gtctgttatt ctggtaatgg ttccctactt 13320
agggtatttc taactatgaa tcaggccttc aggagaaata ttactaactg gaaatattga 13380
gggacgtgga gacaagatgt gagcctacag cacagctttc cttccagtgc gtatggtaca 13440
taggatgccc aagtgcgggt tcaaatatgc tgagatgttg attcgctcag cccttgggat 13500
ggatcttgca atgagacttg ctcagtctca tttgtttttc ccgagtacag ggtaccatgt 13560
acctaaaatc gtttgcttgt tactctggga tatcatggga agccctggcc tagggcatct 13620
agaactacat gcagctagca tgtggaggac tgaaagaatt ctctgaaccg aagagcatgt 13680
gatttggaat ttttaaacat agtttctgta aatatagatg gagtcttccc tgcacatata 13740
aggcatatcc cgtggatcca aaggggtaac tatgtgattt gacagcaaaa gtggatgtga 13800
tccacaaagc agagaaatac ggagccaggt ccaccttgga aaaacaggaa cacatatcca 13860
cactcagtac gcatcctctc tcatctcacg actctcatcc cagtcagagt tgtctgaatg 13920
gacaggatta ctggcaaata tttgctatga tgaagatttt tttcccatga ttgctaatat 13980
tttatgcctc aatcccccat tcctccccac cccagcaaac caagtccttc atgatgtttc 14040
catggctctt gcagttccta gacaatctgt ttggcttccc catccaagat ttttggagtg 14100
gaacactgga atataggata gcatcacaag ttagtcaaga aagtcaatag gaagtgatgt 14160
ttatagctgg gataagatgt ccttttaggc cacttaagct ttgtacatgg acctgcactg 14220
tgtgtatttc atagaacagc taggtagcat ttcccatgtc gtgtctacca catgttggat 14280
gtcagggtgg gttgaaatgc tttggatcag tatttgtcaa attatgatct gtcgactacc 14340
atcttctagg actaatacat taaatatatt ttaaaaaatt ggagttatat gtttaagtaa 14400
tggtcaggat taattctagg gatagaaagt tctagggata aatattcaag actaagtagc 14460
tgagagacac aatcaagaac agaatttttt tctttatttt tttaaactac actatgtttt 14520
gtgtgttggc ttctctacat ttccctcatg gttactagat gattgttatg tctctatgac 14580
tgcatcttcc ttccagagag gagaaaagag gaagtaaaaa ggggttaaaa gtcatgttag 14640
tggagtcttt cccttttatg agaaaaacaa aaaacattcc taggaacttt cctcttaagt 14700
cgcactagac aggatttagt cacatggcta ccatcaatac atctttggaa aagggtaaca 14760
agattaccac aactagtcat ggccagggtg accacctgcc tatcaaagat ttctgtttct 14820
ttttcatggt gtagaatggt tgctaagaag gcacacttcc aagcctttct tttttttttt 14880
ttttttttta attttttttt tttttattat actctaagtt ttagggtaca tctgcacatt 14940
gtgcaggtta gttacatatg tatacatgtg ccatgctggt gcgctgcacc cactaacgtg 15000
tcatctagca ttaggtatat ctcccaatgc tatccctccc ccctcccccg accccaccac 15060
agtccccaga gtgtgatatt ccccttcctg tgtccaagtg atctcattgt tcaattccca 15120
cctatgagtg agaatatgcg gtgtttggtt ttttgttctt gcgatagttt actgagaatg 15180
atggtttcca atttcatcca tgtccctaca aaggacatga actcatcatt ttttatggct 15240
gcatagtatt ccatggtgta tatgtgccac attttcttaa tccagtctat cattgttgga 15300
catttgggtt ggttccaagt ctttgctatt gtgaatagtg ccgcaataaa catacgtgtg 15360
catgtgtctt tatagcagca tgatttatag tcctttgggt atatacccag taatgggatg 15420
gctgggtcaa atggtatttc tagttctaga tccctgagga atcgccacac tgacttccac 15480
aatggttgaa ctagtttaca gtcccaccaa cagtgtaaaa gtgttcctat ttctccacat 15540
cctctccagc acctgttgtt tcctgacttt ttaatgattg ccattctaac tggtgtgaga 15600
tgatatctca tagtggtttt gatttgcatt tctctgatgg ccagtgatga tgagcatttc 15660
ttcatgtgta ttttggctgc ataaatgtct tcttttgaga agtgtctgtt catgtccttc 15720
gcccactttt tgatggggtt gtttgttttt ttcttgtaaa tttgtttgag ttcattgtag 15780
attctggata ttagcccttt gtcagatgag taggttgcga aaattttctc ccatgttgta 15840
ggttgcctgt tcactctgat ggtagtttct tttgctgtgc agaagctctt tagtttaatt 15900
agatcccatt tgtcaatttt ggcttttgtt gccattgctt ttggtgtttt ggacatgaag 15960
tccttgccca cgcctatgtc ctgaatggta atgcctaggt tttcttctag ggtttttatg 16020
gttttaggtc taacatttaa atctttaatc catcttgaat tgatttttgt ataaggtgta 16080
aggaagggat ccagtttcag ctttctacat atggctagcc agttttccca gcaccattta 16140
ttaaataggg aatcctttcc ccattgcttg tttttctcag gtttgtcaaa gatcagatag 16200
ttgtagatat gtggcattat ttctgagggc tctgttctgt tccattgatc tatatctctg 16260
ttttggtacc agtaccatgc tgttttggtt actgtagcct tgtagtatag tttgaagtca 16320
ggtagtgtga tgcctccagc tttgttcttt tggcttagga ttgacttggc gatgcgggct 16380
cttttttggt tctatatgaa ctttaaagta gttttttcca attctgtgaa gaaagtcatt 16440
ggtagcttga tggggatggc attgaatctg taaattacct tgggcagtat ggccattttc 16500
acgatattga ttcttcctac ccatgagcat ggaatgttct tccatttgtt tgtgtcctct 16560
tttatttcct tgagcagtgg tttgtagttc tccttgaaga ggtccttcac atcccttgta 16620
agttggattc ctaggtattt tattctcttt gaagcaattg tgaatgggag ttcactcatg 16680
atttggctct ctgtttgtct gttgttggtg tataagaatg cttgtgattt ttgtacattg 16740
attttgtatc ctgagacttt gctgaagttg cttatcagct taaggagatt ttgggctgag 16800
acgatggggt tttctagata aacaatcatg tcatctgcaa acagggacaa tttgacttcc 16860
tcttttccta attgaatacc ctttatttcc ttctcctgcc tgattgccct ggccagaact 16920
tccaacacta tgttgaatag gagcggtgag agagggcatc cctatcttgt gccagttttc 16980
aaagggaatg cttccagttt ttgcccattc agtatgatat tggctgtggg tttgtcatag 17040
atagctctta ttattttgaa atacgtccca tcaataccta atttattgag agtttttagc 17100
atgaagggtt gttgaatttt gtcaaaggct ttttctgcat ctattgagat aataatgtgg 17160
tttttgtctt tggctctgtt tatatgctgg attacattta ttgatttgcg tatattgaac 17220
cagccttgca tcccagggat gaagcccact tgatcatggt ggataagctt tttgatgtgc 17280
tgctggattc ggtttgccag tattttattg aggatttttg catcaatgtt catcaaggat 17340
attggtctaa aattctcttt tttggttgtg tctctgcccg gctttggtat cacaatgatg 17400
ctggcctcat aaaatgagtt agggaggatt ccctcttttt ctattgattg gaatagtttc 17460
agaaggaatg gtaccagttc ctccttgtac ctctggtaga attcggctgt gaatccatct 17520
ggtcctggac tctttttggt tggtaaacta ttgattattg ccacaatttc agagcctgtt 17580
attggtctat tcagagattc aacttcttcc tggtttagtc ttgggagagt gtatgtgtcg 17640
aggaatgtat ccatttcttc tagattttct agtttatttg cgtagaggtg tttgtagtat 17700
tctctgatgg tagtttgtat ttctgtggga tcggtggtga tatccccttt atcatttttt 17760
attgtgtcta tttgattctt ctctcttttt ttctttatta gtcttgctag cggtctatca 17820
attttgttga tcctttcaaa aaaccagctc ctggattcac tgattttttg aagggttttt 17880
tgtgtctcta tttccttcag ttctgctctg attttagtta tttcttgcct tctgctagct 17940
tttgaatgtg tttgctcttg cttttctagt tcttttaatt gtgatgttag ggtgtcaatt 18000
ttggatcttt cctgctttct cttgtaggca tttagtgcta taaatttccc tctacacact 18060
gctttgaatg cgtcccagag attctggtat gtggtgtctt tgttctcgtt ggtttcaaag 18120
aacatcttta tttctgcctt catttcgtta ggtacccagt agtcattcag gagcaggttg 18180
ttcagtttcc atgtagttga gcggctttga gtgagattct taatcctgag ttctagtttg 18240
attgcactgt ggtctgagag atagtttgtt ataatttctg ttcttttaca tttgctgagg 18300
agagctttac ttccaactat gtggtcaatt ttggaatagg tgtggtgtgg tgctgaaaaa 18360
aatgtatatt ctgttgattt ggggtggaga gttctgtaga tgtctattag gtctgcttgg 18420
tgcagagctg agttcaattc ctgggtatcc ttgttgactt tctgtctcgt tgatctgtct 18480
aatgttgaca gtggggtgtt aatgtctccc attattaatg tgtgggagtc taagtctctt 18540
tgtaggtcac tcaggacttg ctttatgaat ctgggtgctc ctgtattggg tgcataaata 18600
tttaggatag ttagctcctc ttgttgaatt gatcccttta ccattatgta atggccttct 18660
ttgtctcttt tgatctttgt tggtttaaag tctgttttat cagagactag gattgcaacc 18720
cctgcctttt tttgttttcc attggcttgg tagatcttcc tccatccttt tattttgagc 18780
ctatgtgtgt ctctgcacgt gagatgggtt tcctgaatac agcacactga tgggtcttga 18840
ctctttatcc aacttgccag tctgtgtctt ttaattgcag aatttagtcc atttatattt 18900
aaagttaata ttgttatgtg tgaatttgat cctgtcatta tgatgttagc tggtgatttt 18960
gctcattagt tgatgcagtt tcttcctagt cttgatggtc tttacatttt ggcatgattt 19020
tgcagcggct ggtaccggtt gttcctttcc atgtttagca cttccttcag gagctctttt 19080
agggcaggcc tggtggtgac aaaatctctc agcatttgct tgtctataaa gtattttatt 19140
tctccttcac ttatgaagct tagtttggct ggatatgaaa ttctgggttg aaaattcttt 19200
tctttaagaa tgttgaatat tggcccccac tctcttctgg cttgtagggt ttctgccgag 19260
agatccgctg ttagtctgat gggctttcct ttgagggtaa cccgaccttt ctctctggct 19320
gcccttaaca ttttttcctt catttcaact ttggtgaatc tgacaattat gtgtcttgga 19380
gttgctcttc tcgaggagta tctttgtggt gttctctgta tttcctgaat ctgaacgttg 19440
gcctgccttg ctagattggg gaagttctcc tggataatat cctgcagagt gttttccaac 19500
ttggttccat tctccacatc actttcaggt acaccaatca gacgtagatt tggtcttttc 19560
acatagtccc atatttcttg gaggctttgc tcatttcttt ttattctttt ttctctaaac 19620
ttcccttctc gcttcatttc attcatttca tcttccattg ctgataccct ttcttccagt 19680
tgatcgcatc ggctcctgag gcttctgcat tcttcacgta gttctcgagc cttggttttc 19740
agctccatca gctcctttaa gcacttctct gtattggtta ttctagttat acattcttct 19800
aaattttttt caaagttttc aacttctttg cctttggttt gaatgtcctc ccgtagctca 19860
gagtaatttg atcgtctgaa gccttcttct ctcagctcgt caaaatcatt ctccatccag 19920
ctttgttctg ttgctggtga ggaactgcgt tcctttggag gaggagaggc gctctgcgtt 19980
ttagagtttc cagtttttct gttctgtttt ttccccatct ttgtggtttt atctactttt 20040
ggtctttgat gatggtgatg tacagatggg ttttcggtgt agatgtcctt tctggttgtt 20100
agttttcctt ctaacagaca ggaccctcag ctgcaggtct gttggaatac cctgccgtgt 20160
gaggtgtcag tgtgcccctg ctggggggtg cctcccagtt aggctgctcg ggggtcaggg 20220
gtcagggacc cacttgagga ggcagtctgc ccgttctcag atctccagct gcgtgctggg 20280
agaaccactg ctctcttcaa agctgtcaga cagggacact taagtctgca gaggttactg 20340
ctgtcttttt gtttgtctgt gccctgcccc cagaggtgga gcctacagag gcaggcaggc 20400
ctcctggagc tgtggtgggc tccacccagt tcgagcttcc ctgctgcttt gtttacctaa 20460
gcaagcctgg gcaatggcgg gcgcccctcc cccagcctcg ttgccgcctt gcagtttgat 20520
ctcagactgc tgtgctagca atcagcgaga ttccgtgggc gtaggaccct ctgagccagg 20580
tgtgggatat agtctcgtgg tgcgccgttt cttaagccgg tctgaaaagc gcaatattcg 20640
ggtgggagtg acccgatttt ccaggtgcgt ccgtcacccc tttctttgac tcggaaaggg 20700
aactccctga ccccttgagc ttcccgagtg aggcaatgcc tcgccctgct tcggctcgcg 20760
cacggtgcgc gcacacactg gcctgcgccc actgtctggc actccctagt gagatgaacc 20820
cggtacctca gatggaaatg cagaaatcac ccgtcttctg cgtcgctcac gctgggagct 20880
gtagaccaga gctgttccta ttcggccatc ttggctcctc cctctccaag cctttcttga 20940
atctaggtag agccatgtga ctaggtgctg ccaatagaat atgcatggta gttattgggt 21000
tctccaggct gaagcagtga atgagtgggt gtcccctttt tccttgttcc ctctctctct 21060
gtatctcact gtgtgtgagt gtgtgtgtgt gtgtgtgtat ggaagctgga agaaagctga 21120
ggccagggga gaaggtggag agccataaga tgttcatgag cattacctga caagtacccg 21180
cttttgttgc tgtgtgaaga gtaagtgaaa tttttatttt gttaattcaa ggagatttta 21240
ggggtccatc tgttatagta gctaccctta ccctaaatgg taccctggtt taggccagtc 21300
atgattcatc ctctgaaagt agagtgacag ggcctatttt ccttaagttc ttgatgtctc 21360
cctgctctta acctattaat tgagcctctg ttaccaggta tagatattat aacaatagta 21420
gaaagtgtct cccacagtac gtcataacaa tttttgtatg cctgttgaaa atacagagcc 21480
cttaactctt ccccaggatt actgaattag gctcagctgt ctacatattt aaaagtactc 21540
taaatgattc taatgtaccc tgaatttgga cacaactgct ccataaggtg tcatggagca 21600
gcaacccatg gatcatcaat gtaccgtcct cctccagagc attgatgtgc tgagaagtag 21660
aaggtattag aggcagaagc catcattcct tacaagggag aatgggagta gtgggtcttg 21720
atgccgacag cagacacagg gatgatgtcc accgtaacct ggataacgtc ataataatgt 21780
aacatagtaa atatgattta aaggaggagt accttcaaaa taagacagga agaagcctgt 21840
caagtcactc aagctcttct ttgccccaaa acagttgcct atgacacttt accaaagcat 21900
tattacttaa aggttttaaa gtaacataga ccagcagcac caccttgcat gccatatttg 21960
agctttttaa agagcccatt gcctagtaag ggtacatgaa ttcagtaagg cataggtctg 22020
ctagacacag gcattcaacg ctctggagga gattatatga aactaagagt tgagagtcac 22080
ttttgagtga tgtagcgacg caaataagaa ttttcaaatc tgtcaaaatg taggctgtta 22140
agtttgacct ggggcactaa gtctgaaggg ttaacagaga agtgatgagc caatgtaaag 22200
ctgtatacac acgtgcacac acacaggagc tgggtgagaa tgttcataaa gcaacaggga 22260
aatgtggtta gtgagcacct ggcaaaatgg ctttacaaat gctttccctg agttttaggt 22320
agaggaatgt cttgttggtg taagcataca aaccaaagag ctctgaagta attcactatt 22380
gccttgaact ttaggtgacc caaatcaagg aataagggat agcttaattt aatgaaagta 22440
gaaggtaaaa aaaaaaaaaa aaaaaagttc agtctatttg gtaagaaaac ctctttggca 22500
aacagcagac atgatgttgc tgatggaatc tttaaagagg tagcggattt ggtgaaggac 22560
ttgtgtttgg catggaaggg ctgggcaaga agaaccctct ccattgcata gcagagggtt 22620
ccccctttca agcgtgctta gggagatgga gttagtggct gaaacacaaa tttaagccct 22680
ttgaaatgta atatcctacg ccctctggtg gttacatttt ttaaaaatcg tactaaagaa 22740
aacaggttga agaaatcata gaatgccaat tacaggaata acagggatct taagtgtcat 22800
ttgtcagagc tgtattcaag atgcccttat cgttagttaa aggtgtcagc ccctgaatca 22860
gaaagaaaaa aaaagcctta tttaatcagt tttgcaaatg cttgagaagt cagctttctc 22920
ccggtttttc tccatcactt ttcaggaact tttccattat gcctctgtcg tcagaatact 22980
tccgagattt ggaagatagt gactccactt gggatggctc tgaggtcctg ctccttcttg 23040
gtattttgat agaaaagcct tttgccactc aaaggggaat ttctaaaaga aacttcaaac 23100
cacacaatta ctttaaaagg gtgatcacac agtaatacat tgaatgatgc ctggccagga 23160
tctaacaaac ttgtctgttt ccctctttgt tgtgtctggg agacagaaag acagcctggc 23220
cattctcatg gcaaactgtc attaaattat atcaagtgta aatagttgtg actgacactg 23280
ttaacaaaga acaggaatcc aggaacaaaa gggcacttca atgaagatta gatgttcctt 23340
aagcaacaga ttttaaggac ctagattcca taaaattaga gttctgatta agtcttatag 23400
atatatgtgt atgacatatg caagaggact tagataaaaa tttcagagaa aaattgaatt 23460
aaagataaaa attaaaaata caagctttgt caacataagc ttcctcaagt tcaggacact 23520
tttgtaagtg atgatacctg ccatttagtc cacctataaa aaaccgatgg tccttggaat 23580
ttaaccatgt caatgcagtg ttttttccat attagctgaa gaaaaatagg taccttaaaa 23640
ttttttgttt taggatcaga aaacaaaaag aagtcagaag gatccagatt aggactgtaa 23700
catggatact taatgatttc ccattaaaac tctcataaaa ttgtctttgt ttattagagg 23760
gatgagcagg agcattgtcc tggtggagaa gcactctttg gtgaagcttt cctgtgtgtt 23820
tttctgctaa agctttggct ttctcataac actcttatca taagcagatg ttattattct 23880
ttggccctcc agcaaatcaa caaacaaaat gcctcaagca tctcaaaaaa ctgttgtcat 23940
gatctttgct cttggctggt ctactttggc tatgactgga ccacttctat ttcttgggag 24000
ccattatttt gattgtgctt tgacagcagg acacactaat aaagccatgt ttcctctcct 24060
gttacaattc tttgaagaat acttcaggat cttgattcta tttgtttaaa attttcattg 24120
aaagctctgt tcttgtctgc agctgatttg tgcacaacag tttttgcacc tatcaagggg 24180
aaagtttact cacctttaat ttttcagtca aaattgtgtg gctcacgcct gtaatcccag 24240
cactttggga ggcctaggca ggtggcttac ctgaggtcag gagttcgaga ccagcctgcc 24300
caacatggcc aaaccccatc tctactaaaa atacaaaaaa ttatcagggt ggatgtggtg 24360
gtgggtgcct gtaatcccag ctactcggga gtctgaggca ggagaagcac ttgaactggg 24420
gaggcagaag ttgcagtgag ctgaggttgc actccagcct gggcgacaag agcgaaactc 24480
cgtctcaaag aaaaaaaaag aaaagcaaga aaaaagccgt gtaaactaaa cgaattgaaa 24540
tatctattgt gttggctgtt ggttctgcta ttaattggtg gttctcttca attaaggcat 24600
aaacaaaatt aatttttttc ttgcaaactg atgtcgatgg tctgctcctt tgggctcatt 24660
tcaacatcat ctcattgaca atttaaagtg agttactcat ttataaatta ctgatttatt 24720
tgggacattg tttccataaa cttttcataa agtgacaatg attttgccat tctttcgccc 24780
aagctttacc ataaatttga tgtttgttct tgcttcaatt ttggaagaat tcatattgct 24840
ttgataggga atcatttcaa actcatgttt tgtaattctt agtgcctcta attagatcct 24900
gttcagtcat attgtaacaa attggtacaa gtttatttgg tgcaaaattt tttttgaaat 24960
ctatacgtag attttgcaaa ataaacattt tccataaagc ttctgacaac cccttattta 25020
tagcaagatc tttatttagc tagatatgta aagctagaat atacattcat acaatgcaaa 25080
cagcataata tatgtttaac aaatatttac gaataaatca aaggaggaga aatctgaaaa 25140
ggtcttatat tttgtgccta gatttttctg aaaattcatt ttctaacaaa tcatacggtg 25200
attttgattt ggtggtggga agagtggtgg gtgggttgga ggatgttgtt aaattggaga 25260
aaatttagat tcagagcaaa ataaaaggta tctttaaaac attttttgtt ttttaaattt 25320
gtttgtattt tctattttca tttttttcta catttaaata agaaagtttg tattatgcaa 25380
atcccaggaa ctgtattaag ttgaattatc actatagagg tgaatggttc cgtaaaatac 25440
agttagtgga accttgttaa aaacacccta atatcctaaa atgactttga aatgtgatct 25500
gaaaaccctg tcattttcca gtttgaaaat gtagaaatct aaaaccaatt ataatagaga 25560
aaaaaattta agtgaatgtg attttgtgtg ttgttaggag tcccttcact ttcctgtgta 25620
caattggctt tcctatatga aagcacttgc atttgatggc ccgtttataa ggaaaacctt 25680
tgattgcaac cttttggaat tcgttatgtt tatttccagc tggacatcaa tttaactgac 25740
cattgtgttt ggcttccagt tatgaaatga aggaatctct aggtttaaaa atcctaataa 25800
ttgtttctga agcagcttta accactggca gtccagcaga tattcactga ggcactagta 25860
tttctcttga gttaaaagaa aaagaaaaag aaaaaagaat gaagaaaaaa aaggaaggaa 25920
ggaaagaaga gagctggaga gagggaatga gggaggggga aaggagggag agagggagaa 25980
agggatattt agcagctctg tagatttctc ttaaaaagaa acacttaaac aatgtacttt 26040
cacacatttg tccagtgcag ttactggcat ataactgatt cacaataatg atttttaatt 26100
aaactcttgg tcccagtgca aaaaataaat atgcccagaa aataaaaata taaaagcctc 26160
accaaaactt taataacttt aataaacttt tattatgtta attgacatat aattgcacag 26220
atttatgggg accagtgtga tgttttgata catgtatgca atgtgtaatg atcaaatcaa 26280
ggtaattagc aaatccaaac cttaaatact tattatctct ttgtgttgag aacattcaaa 26340
attctctctt ctgattgcta aaaaaataga ataaattgtt aactacagtc accttacaaa 26400
tgctgtgaaa cactagaact tattccttct atcttgctgt aattttgttt tggttaaaca 26460
acgtctcctt atccctgctc acttttcatc ctttagtaac cactattcta ctctctactt 26520
ctatgagatt gacattttag cttccatata tgagtgagaa catgcagtgt ttatctttct 26580
gaacctggct tatttcactt aacacagtgt cctacagatt catccacatt gccactaatg 26640
atagaatgtt actctttttt aatgactgaa tattccattc tatattataa ttatatataa 26700
tggagtaata tgtatttata atatagttgt ttatatatgt gtgtgtgtgt gtatatatat 26760
atgtgtgtgt gtgtgtatag ccattatgga aagtaatata attatatatg tatatataaa 26820
atcacatcta tatgtaaaac tttttatttc tttgttcagt cttgtaggtt atatgtatcc 26880
aggaatttac ctatgtcctt taggttttct aatttgttat tttacatata tatatatata 26940
tatatatata tatatatata tatatacaca cacacacaca cacacacaca cacacacaca 27000
catatatata tatataaaca ttttcctttt ccattcacct gtagatgggc attgaggtca 27060
tttccatatc ttggctgttg taaatagtgc ggccataaac attagaagcc ttgtcttcag 27120
gttcagaaat tcttcttttt gatttagtct cttattaaag ctattgattg taattttttt 27180
tgttttttat tatgtttttt ggctccaagg tttttgttta gttcttttaa aggtatctat 27240
ctcattgttg aatttttctt tcaaatcacg aattttttct ttttcttttt tttgagatgg 27300
agtctcactc tgtcacccag gctggagtat ggtggcgcga tctcggctca ctgcaacctc 27360
tgcctaccgg gttcaagcga ttcttctgcc tcagcctccc aggtagctat gactacaggc 27420
gcgtgccacc atacctggct gatttttgca tttttagtga agacagggtt tcaccatgtt 27480
ggccaggctg gtctggaact cctgacctca agtgacctgc ccacttcagc ctcccaaagt 27540
gctggaatta caggcatgag ctaccgtgcc tgacccaaat acccaaatca tttttttttt 27600
ttttgctcta ttttctatct gtattctctt gtatcctgta tagtttcctt aagatcattc 27660
ttttgaatta tttttctggc atttcatgaa ttttctttgg ggtctgtcac tagagaatta 27720
ttatgtgtct ttgaagctgt catttttttt ttttgcttgt ttatgtttct tatgtcccta 27780
tgttgatatc tgcacatctg gtggcaaact tgtccctttc agttttatgg agtagctttt 27840
gtaggaaaag acttattcct gtagatgagc tctagcatat tggctgggta agatgcactg 27900
gttttggttc tgggttgaca cagtaatata gtttagtttt catgcaattt cttaggttat 27960
attttatgtt aacaagatat ttgcaagtgt ctaagtggcc taggctgtgg aagtttgtga 28020
tcatggtggt gtagctttgc cagctggccc tactgggcct actgggctga ttctcaggcc 28080
acagacatgt aaattctcat cagagtaagg tcactgagat atttccctta ccaggggtac 28140
agacatgcag acgtgtggca gggtatccaa ttcagaggaa aggccacatt ggatgcgttt 28200
gctaggctgt ttctctggct gagaacataa ggatgcagca gcttggcccc tggggaatga 28260
actgagtgct ggaggtgggt cggcctgcta ccctagccca aggtgtgggc acatggtagc 28320
ttggccagct tggaggaaag ttgaacttat aaatattgtg acatccaaga tttattttta 28380
tttcaaagga ttaaaattaa tagttgtaac agtccactgt gaagtaaaaa ctacccaaat 28440
atttattgac aatagaatgg atatataaat tgttgtctaa tcatacatta gagtaatatc 28500
caacaatggc agtaaattat ttgtaattgt atgcaacaac atggacgaat ctcaaaacat 28560
catgttgagt aaaagaagcc agactcaaga gtgtacatat tgtataatac cccttataga 28620
ataaacaaag ttgtgcaaaa ttactctttg ctattaaagg gagaaatgct agtgacagaa 28680
agtgatcatg agacattttc tttgatgcta cttaagtata tgcatttgag tatatgcata 28740
catattttaa agatggcatc taaagtggtc tttctaaaac aaaaatataa tcataaaaat 28800
ctcatgccca aaaagtttat actctaaaag gctcaccatt tcaaacagat aacatccgcc 28860
taatctttaa catggctaac ccaatttttc atatcgactt ctctgacttc tcttggctta 28920
tctttctttt ggattcctta cccaaacctt tatatattca tgtacacctc actaattatc 28980
cacagttttc ttattattca ttaaattccc cacttggtct ccttcttaca tgttctcccc 29040
aatctggtta gagatctctc ctacttcctc ccaaagcacc atgtgtttcc ttcagcttag 29100
cagttaccac attccttggt aagtactaat caatttgtat gtttaccgga ctcagactgt 29160
gacgacaagg accttattta tccccactgt aaagtcttgt gcctggaata caaccgaatc 29220
tgaatatctg ttttctgtat aactaattct atcatggatg agatttttat aattattaat 29280
gtgatgtttc ttcttttgct attttattta taaggctttt tgtctttatg aatgtacaat 29340
tgggacctct gtttgtgtga gaaaattaag atttattata taaagtattc atgaaaaatg 29400
ctgcatgtta gaatattaat tcatcttcta acatgcagta tgtgagtatg tgtgtttata 29460
ctgtatatgt attctttatg cagatataga aagtaagcta atattgactt ccctcttttt 29520
tgtcattata aggttttata ggaaaccaag tgttttctca agaaaaacta agaatcacct 29580
taaaaattag tttgagttac ccaattgtat attagttata ttattttact ctgctaccca 29640
tgaggcaagg ttaaagacag aaggctagat aagtgattca caaaaataaa gataatagaa 29700
tatatatata atatatatat acatctccaa gttgtactgt gagtttaaat atatgaccca 29760
cttactaatc aatcaatgga gaaaagctat gacatatttt taaggtgaca tgttagaggt 29820
atttcatgaa tatttgagat ttgataagac acacatatta ggtattagct aagtattcct 29880
aattattaat gtaaactaga ctagaatgtg cattagaaag agtagtagat atattgtcac 29940
aaaagctgag tgcgaaaagc aacaccagca acttccaggg tgatgccgaa tatattattg 30000
caggcttgag ttccctcatc tctaaaataa aagaattgga acagctgatt catatgttag 30060
gctaatattc tatgctcttc cctgaactga aaagcctctt taaaagtttt tttaatgtaa 30120
tgatttagct ataatactgc ctttaaaaag ggattgacag aggaagcatt tgaatttcaa 30180
tctggcctta catgcatttc ttttgaaaag tcattagaaa tgtctttaga aaggtactat 30240
gtgtttttaa gggcaacata ttagttattg tagtttttga gcacatttcc ataacacatg 30300
tgttgtgggg tgttaccacc accccaatca aaacataaaa tgtttccatc ttctcataac 30360
ctttcctctc actacatttg gtcaatcctc ttgtactcta tcaccatgga tgaattttat 30420
ctattttaga acttcatata aattgattca tgtagtatgt tctcttttgt ctcatacttc 30480
cctcattcaa aataatgtct ttgagaatta gtttgtgtta tttgcctatc agttgcttgt 30540
atgtttttta atttttattt ttcaacttag tagtcttcca ttgtatacat ataccacaat 30600
ttatttctcc attcacttgt tgataaacat ttagtttttt tttctgatat ttggtaatta 30660
taaataaaaa tgctagaaaa attcttgaac aagttttttt gggagacaca tgtttttatt 30720
cttctaggat aaacatctga gagtggaatt gttgagtcat ggacttaaca catttttaaa 30780
ataaaatttt aaagtaaaaa taatttaaaa aagaaaagat ctttcatgaa tttagtagtt 30840
tatttctgag tgtaatcgac ttcattattt tggccttttt tttgatgggg gggtggtggc 30900
tggagacttg cctaacttat gggaatctgg aattgccctt ggcattgcat gtggcaacac 30960
aatggtacag aagcttttgt gaaagttttt gtttccatga atgatgggaa ctgccaaaat 31020
tgagaattat gcactctgag tgaactttca catagcttag gttggcatca ccaagtgaga 31080
agcccaggac ttaaaaaata aggtaaaaca agacaggagg tttttgtttt gtttttccta 31140
gactatggct caaggaccac ctgaacggaa atcccctggg tagctgatta ggcatacttt 31200
tttttttttt ttttttcgag atggagtctt gctgtgtcac ccaggttgag tgcaatggca 31260
tgatctcagt tcactgtaac ttctgcctcc tgggttcaag cgattctcgt gcctcagcct 31320
cccgcgtagc tgggattaca ggcacccgcc accaagcccg gctaatgttt gtatttttat 31380
tttagtagag atagggttct gccatgctgg ccaggctggc ctggaactcc tgacctcagg 31440
tgatctgctc acctcggcct cccaaagtgc tgggatcaca ggcatgagct accatgcccg 31500
gcctagacgc actttttaac aagcatgtaa gagactctta cacatagtga aatgtaagaa 31560
ttgctagatc aatctaaacc agagatcctc tgctgtggcg gcacatttgc atcaccaagg 31620
gagctttcaa aattgccatg cccttgacat gcctaagacc aattaagtca ggatctctag 31680
gcatgaccac cagatatcag tattatttaa gtctccccaa gtagttacaa tgagtagcca 31740
aggatgagat cttctggtct attctatgtc atccttagca catctgagag ctccactgcc 31800
tgcatgtctg atgttttgtt gtacaactac gtcattctga caatatttca aagcattgaa 31860
agaacattta tttcctttgt acaaaatgcc ttgagcaagc aagattgtct caggtgcatt 31920
tgccgtaatt gcttgccttg gtcttattac cttgtgaaaa attatgtatc tttaatctat 31980
gctttgggtg gcaaaattaa ttaataatta actttgtact tacctaataa aataatgttg 32040
atgtaaagtc ctctaagtag tcctctcact tactaatttt atctatgtat tggcatgaat 32100
atcttggttg aaccaaagaa ataaaaccta aaatatatgc ctcacaaaaa tgtgctttat 32160
ttaagtagat tcaatgaatg agatatcagc tctcctcaaa tcattataat gtacctgtaa 32220
gtaacgaact ttcataatgt atttaatcta taaaaatgat tctatatgca gcttttcagg 32280
aatgcatgca tatatttcag aaagtaaaat tccctttttt tccaggggaa gacttactaa 32340
gatattccac ttgaatgtat gaagtacttg ataaagtgca tttctgcttt acatggttgg 32400
aattctactt tagtgctaac cttgcatttt taaattttgc taatagagac catataaaaa 32460
ttcacctgca aagcaactta aagaatgttc ttatttatta tttgtaaatg tggcatcctt 32520
tacctatcat gcacaccctg caacatctcc cccaattaaa tagattttgt tcttcaatga 32580
agtacaatag tttgatggat tacatttagg aaaacacgtc agtcaatatg aaccaccgaa 32640
tcccaaaatg ctattcaaat acccttatga tctctttaac aaacaaaagc cttcagacat 32700
catgtaagaa ccattgcatc tttacttttt aatgcaatac aattatttgt gcctacctaa 32760
aggtaaccag atacaggttg ccttttgaag tttgcagtag cttcatttta ttctgaacca 32820
gaaagttagc cacaccagag aacacttttt tcccattgtc ttcttcaaat gtggttttga 32880
ttgcccattg acaaagccaa gctagtgatc agtatattgt gattctccta attaaaagca 32940
tgctaacacc tagaatacag tagttgtccc ttatccatgg gggatatgtt ccaagatccc 33000
cagtggatcc tgaagccgtg ggtactgaac cctatatata ccctgttttt ttttctatac 33060
atacacacct atgactaaat ttaagtataa attggacaca gtaacagatt aacaacaatc 33120
actaataata gaaaaattat gacaatattt tgtaatagaa gttgtgtgaa tgtggtctct 33180
ctttctctca aaatatctta ctgtgatgta ctcacctgtt ttcagaccat ggttgactgt 33240
gggtaactaa aaccatggca accaaaacca gggataaaag ggcactactg taaaccattc 33300
ccatagtccc acaactccca tgataaagac ttcacactat aaatgaaaga actgtttgat 33360
tggcacagac ccatctttga gagagcctgg agagcctggc caccctcccc ccacaatatg 33420
tgccaatgtg ccaatcatgt ctctttggaa tttcacaggc catagccttt gttatgtgcc 33480
cttaaaacag cccctgtcca tctaccatgc tgttggctct ctgccatcct ttctagcact 33540
cttaaaacag actataattg gcatttgctt ggctgggagt caatttattt tattgctacc 33600
tttcccagtt gccttaaccg ttggatgtct tgacagttgg caaatgtaga ctgtggttct 33660
gatatgtcac ataaaatctg ttggatgaat actttcctct aggaaaaagt gaaagtgaaa 33720
tcccatcctg gtccaccctc tttccaaaac aataaacatt tcctggcttc cacaattccc 33780
ctaatcattt aaagcagtac agttgttatg gaatgggcag ctgaacttaa tgcgtgtctg 33840
gaccatctgt tctagtattt agtcttaaag aagattgctt aagtgactac agttcattgc 33900
aaagagggtg agttccatca gggaaagtgc tagggtacta tgtcatagaa aaacataatc 33960
cagagactct ggaaggacta tttttcactt agcaatgctt aacatccatg gaaaaaaata 34020
aattattttc ctaggtacac aagtaataca cagacaaact tgtctccaaa tttgtgattt 34080
gtgttacatt atggacacaa atatgatatg gttgtagaag agacacaaag agttttatcg 34140
ctttattttc ttgagacata tttttttaat tatagaaaca tcctaagttc aaaaagtcag 34200
acaaaggtca gacaatttac acataataaa tggcatgaag tagataggca agaaatactg 34260
agattataga ggtaagaata ttttattaag tgaaattcct cacatagctt ttaactatga 34320
cattcactat atttctgggc cagagatttt acaaataatg aagtactttt gttgtttcaa 34380
cagactttac cacaaggttc tggttttgtt taacgttagt gtaggacaaa ttaagactgt 34440
ccagtatatg aaaccttcaa acttgagaat atataaacag tacagacatt atggaccata 34500
gtactcactc aggaaactaa ttttttatgt gatctcacat catccaagtg catgactcag 34560
ttcagtcaaa tgcccagaat ataaatggaa gcaaggctag tagtaacagt gacagagcaa 34620
gttgttataa gaagaacttc caattatgaa gcatccaaaa atggaaattt ctgcctcttc 34680
aagcaggaag taaatcaaat ttgaaaatat tagggaaaaa aagcctggga gtcttcaaat 34740
gagatacata ctttggatga gggatttgcc aagatggcca ttgtgattct agagcgttaa 34800
acaaattgta ccttaaaatt gtataatgta gacagtcgtg tgtgggccaa gtaacttaat 34860
gaaagacaaa tagagtttcc tttttctttg tgtcattgag aaaaatatat atattttaaa 34920
tttacttcta ggaatattta ctaattgaaa ataattcata aatggagtca aatagagttg 34980
acaatgaatg aataaccaag agtagatgga aagagattag taaagataag caattgtgga 35040
ataatcttat agtacttttg agtgcttttt attttgtgaa ttaaattatg tgaaatgata 35100
aatgatttat agcataatcg aaagaagaat gacaccacag cgaaggccca tattgagcag 35160
caagtcaact gctagggcta cgcgaggcaa agttggttgg caaccaggtt cctcaatagc 35220
tgtcatgctc cagaaaagag ccacttctac agcaggttcc agaaaaaata aaggggatag 35280
attaacgtaa acattcaaat aaagaaaaaa aaagacaaca cgtaaggcgc aaacgttccc 35340
tgctagtttc tactgacatt ttttattaac attccttcag tttgttggag gagacagtga 35400
tttaccttaa aaatatttcc tagcctcact tgaggttgca ggtgaccgtg cgattaggct 35460
ttagccaatg aaatatgaat gagtgtccca tgaatgaggg cttccagaaa gctccatgag 35520
agtagatttg tgtgaaaagt tcattttgct cctctctttt ggaataagga ggccaatttg 35580
aaagcacaag aaacagtgaa agaagagcta caaggagcct gagttttcaa tcaccatgtg 35640
cttgcgtgct agctttgggt tgtctacttc taaacttaat ttatgtgaga gaaaaataaa 35700
cttctaatct atttaagcca ttgtgtttta gttttctatt tttatcagtt ctaagcagtg 35760
tcgaattgat aagagcacag acctgttatg acatctatgg catagtttat ttttctgttt 35820
gtgaagaccg tcacttatat aaggagaaaa catctcttta taatccagtt taccatgtat 35880
gatagattta ataataattt atggaaaatg taagaagaaa gtaattttaa gacatcaggg 35940
gaattaaaga gaagaatgct ttgaacattt tactttgtct gattgtaatt gaactgaaat 36000
gtatttccta tatcttgaaa aaccctctca gttttaaatg tggtcatctc agtttcttat 36060
acagtaaatc tctgccccca aaacatttat ccaggatttt gagaatctgt cccttgtctg 36120
taggacgtta ccgtagagag tcctcttcaa ttcttggttc actctttcac tgttcctttt 36180
taatccatct aattcattca tcctcaaaaa agcgcttaag catactgtgt aacatactca 36240
gaattatgct aagtactatg aaggaagcca caaaacacaa aacagcatgc gtattcagct 36300
tgatgatgtc atgtatctct tctttgaagc ttgggtagta ctttataaca tcaagtagga 36360
aatatttttt taaaatttta ttattattat actttaagtt ttatggtaca tgtgcacaac 36420
gtgcaggttt gttacatatg tatacgtgtg ccatgttggt gtgctgcacc cattaactcg 36480
tcgtttagca ttaggtatat ctcctaatgc tatccctccc ctctcccccc accccacaac 36540
agtccctggt gtgtgatgtt ccccttcctg tgtccaagtg ttctcattgt tcaattccca 36600
cctatgagtg agaatatgcg gtgtttggtt ttttgtcctt gcgatagttt gctgagaatg 36660
atgtaggcaa taattttaac aatttgaata gctcagtagc tcagtcttta cttttttttt 36720
tttttttgag acagagtttt gctcttgtcg cctaggttgg agtgcagtgg cgctatctca 36780
gcaaactgca acctgtgcct cccgggttca agtgattctc ctgcctcagc ctcccaagta 36840
gctgggaata caggtgcctg ccaccgtgtc cggctaattt tttgtatttt tagtagagac 36900
agggtttcat catgttggcc aggctggtct tgaactcctg acctcaggtg atccacccac 36960
cttggaaatt agaaatgact catggaaaat tctttatttt gtgtccaagg aatcaactgc 37020
tgaaatacag attaggtgaa gattgaattg agaagagttc aattgaccca tgtaaaaaat 37080
ctgcacatgt accccgaacc taaaataaaa gtagaaaaaa aagaaaaata atccccccca 37140
gagaatacta aaagcccaaa gtagagattttatttgacaa tcagcattat acataaaaga 37200
ctgattcaac agctaagcaa gagcagtggg gagcatgtga taagaacgac tcacatttat 37260
tgacccttta gtaatggctt ggcatatgct ggtttaccta tatgtattgc ccatttaata 37320
atagacaata ataatgtcac tgcatcctgt gcttgtcaat caatgtccag agagtaatat 37380
gcaatggtag gtgacagttt taagaaggct gagcaaccta tatctagaga agaaggactg 37440
caatgggaaa gattaggcat catggcattt gagatcagga aagaaaatga gtgagttcgg 37500
aatattcaca atcagtgggc ccagatcata acccaattct atttaatcta atacaagctg 37560
ctgatcttta agtagctaca gactccatca acttcaaata tctttgagac ttgtaaatct 37620
tccttgtcat tgtattttgg aaagtgttgc cctgacactg gcccaagtaa ccacagttat 37680
tttctaaaaa tggcaagcat agttctccat aaggagttcc aggcaaaata tatgacctag 37740
acttttcaaa tataccaaag aagattccct tagactttct gacttccagt atgggacatt 37800
gaatggttaa gcaaaacaat cttgtattaa ttttcttgat tcattagaaa agcagccttg 37860
tgtttaatat atttacaggg gcatagatta ggaccatttg ttttggttcc acctgttaac 37920
gttgatcaat tatctgtttt agcacccaac tacataaaag atttacactt tctttccatt 37980
ggaaaacatt ctttgaaatc cacacattca ttttaggatc ttctgtaaag actctttttg 38040
tgctatttat agaaacataa gtccaaagca ctggatgttt caagcatgaa ataacaataa 38100
tagaaaaata ttctaatcca gtcattaaca cctttaggtg ttcagtcagt tagattagca 38160
tttcattcat ataggctgtt tttagagtct caaaattaat ctatgagtaa taataatatc 38220
ctaaagacaa acatatatag attgactttc aagtttttgg caagagaaga cctcagaata 38280
attttaatca agaatatttc agtccatacc cttttagttt cagttatgaa caaaagaatg 38340
catatactat gcatatattt tatcaatatg taatcaattc aacaatgcaa caattattgt 38400
actaaagtac tgttggaata taatatgata ctttcccaca aggaggtccc aacatagcca 38460
aagttgatga gtaagtaggg gattgagtta agaagtatta ctttggcaaa aaccaaagca 38520
tacagactaa tggataccta tgaaactaga ttctttttgt cttctaaatg gctctaaagg 38580
caaatccaaa aaggaattcc caaaattgtt tgtgagttga gcattattgt ataagcagaa 38640
acgttttgta ttaaaagcat tttgtatatg cagaaatata caaaaagcat attgtataag 38700
cagaaacatt ttgtattaaa agcattattg tataaacaga aacgtttagg aggtttcaac 38760
ttaagatgct gttacgctat gtatctaaga gaaagtcatt tctgttttct cattctcatt 38820
tttctcattt aaaaaataaa gaagtaagaa tacatcatct ggctgggcaa ggtggctcac 38880
gcctataatc ctggcacctt gggaggctga ggtgggtgga tcacctgagg tcaggagttc 38940
gagaccagcc tggccaacat ggtgaaaccc tgtctctact aagtgtacaa aaattagctg 39000
gtcatggtgg cccatgcctg taatcccagc taccccggag gctgaggcag cagaatcact 39060
tgaacctggg aggcagagtc tgcagtgagc cgagatagcg ccactgcact ccagcctggg 39120
caacagagcg agattccgtc tcaaaaaaaa aaaaaagaaa agaaaaaaga tctgattaac 39180
atacttttga actgtcttca gctaacctaa atcatttgga gatgtgtttt atgtgttaga 39240
aaaaaaaatc ctcaatgata aagactaata aagtatcttt aaatataaat atgttacttt 39300
ttatgttgga atagttattc tacatcaggg agtgtgtgta tgctaatgtg agccctatca 39360
aaggatgaca ataattcagt tctttcattt cgactccaac tttccactag gaaatacacc 39420
catgttcatg agtcagaaga agaagaattt taaaactatg catatcttac tgtggagatc 39480
cagttcatta aagtaaagaa caggtaaaga gctgaaaatg gaatggaaag aaagcaaaaa 39540
agtatctgtg ttttctactt aatttatcca ctgttaagcc catcatttta atctatgttc 39600
tagtaagaag ctatataaac agtctgagaa acaaaccaac ccaataaaat tgatcagttc 39660
agtcccacac agatattttc agaaatcttt gattattcta ccttaaagca ctttgtctac 39720
ttaaatggta cacaacagta aatgatacac aagagggagt tgatatatat tattagttac 39780
actttataga agaaatatta ttttccaggg cagcaaccca gactctgcag taaacaccat 39840
aaatcaaaga caccataatg ctgatcagaa gctgtcctta agccctgttg acaaggctga 39900
gtactataga gtggattaac ttcttccacc attgcttgtt ttagctgtcc ccactccatg 39960
ttcagataga cactctcagt tcaaacagga gtgagcaaaa atatattgta gattcaaccg 40020
gtggaaaaat aactttacag cagagtttca ttttcagtag gaccattttt ttctgggacc 40080
tctctgtgca cacaattaaa tgaatgctcc ttgattaaat ataaaacttc tgggaaatgg 40140
tcgtttaaat ggcataattt tatgtaacag atattttaaa gacaagtagg acatttagtt 40200
catgataaaa tagggacact cgattaaacc atcaaattat aagattttac tatgtaacat 40260
tatctggata attctttttt ttttttttta agacagagtc ttgctctttc gcccaggctg 40320
gaatgcagtg gcatgatctt ggctcactgt aacctccacc tctcgggttc gagggattct 40380
ctggcctcag cctcatgaat agctggaatc acaggtgcct tccactgtgc ccagctagtt 40440
tttgtatttt tagtagagac ggggtttcag cgtaatggcc aggctggtct caaactcctg 40500
acttcaggtg atcagcctgc ctcagcctcc caaagtgctg ggattacaag cgtgagctgc 40560
tatgcccagc ctggtaattc ttatatgaaa cacaggaact atccatttca tataggccag 40620
actagttttt cccatttaaa atgagtatgt gctttctgaa tttttcatct gcactttggt 40680
attattttaa cttgtaaatt agtttttgaa gcactaccat tcctatccca tgacttggag 40740
ttaaaccaca tgtgcccttt ttggctaata atttacacag ctaacatttc aggttgaatt 40800
tacgtgagta aaagtaaaac agcatttttc cattcttttc aaattcattc cgataataaa 40860
atatttaaat tctggtaaat gacgtaatgg gaaaaacaat gttttctgaa attgtaatgg 40920
tagaccttag tgctgataac aaatgtgtcc aaatatttca caccatttca gatagacatg 40980
tgcatttatt tggaaggctg agctgttatt aaaaacccat ctattgtcat ccatgtctca 41040
gaagagaatg ccaatggcaa tttttcccat attttgaaaa aactgaagat gtatcacttt 41100
taatgtccta cccaattgct tagaaaggaa gacatctctt attttttcca tattaaagct 41160
cttctcatgg ggacaattaa atgtgcataa attcctcaga gtttttattg cttttgttca 41220
atctgtagtc tcaatcagaa atctccttca cctttgaaat acctaatggg tcctgtcact 41280
cttgaggttt actgtagaaa ttataatctg tgcactattt atagcgtgta tctttttcaa 41340
ctatatagac atgtctctgt ctccaaaaga aaatgcaaag aataatacca tcttccaaat 41400
aattttttaa ccccaaatgt tttatgcaat ctatcatgtg ctgagtatgg tcttctaggt 41460
caaaataata cattgctggt aagataatct atgacaaata ttataaaatt aatttgaaca 41520
aaacagtcta attcagagca tgatagctgg caaatacaat aactctgtaa gctatgatgt 41580
tcccagtgtc tcatttttcc cttacatcac acattttgta ttcaatagac aaattaaaaa 41640
agaaattggc aggtatgagt acatggcagt gaataggaag gaagatattt agacctctgt 41700
gtctggcatc tattttaagt ctgatgtgat accatttttt tgcttaggca aatctcgaag 41760
agtatgactt catgaagaat tttttttttt tcagttcaga cctgcaaaaa tctaacttca 41820
taaaacaaat gtaagtttac ttttagcgct atgccagtgg tttcttagat ttaggataca 41880
taagataata tatttgtctt agacagattt cacaaacata tttaacttct tgatttcaga 41940
actttgctgt gaaatagtat cctgcaacta tctggcatac taggatgatt tccaattcaa 42000
tttaatcaat attaaatggg aacttaatgg gcagctcact agtgataatg tattctcata 42060
ctcagaattt acatcttaca tataattata aattataatt cttaaccatt tttgagaatg 42120
aagaattatg tccttctctt tattaccacc tcttgcttcc tagtctcctt ccttggccca 42180
cactaatctc acttaatgag gcttaactat ttcttcagtg ttgtatagta tttatcggtc 42240
attctcatta taggtaatct tattggctaa agtctagata gatgattatg atttactttt 42300
ctttcaaaat taaaggtatc ctaaatctga ttgcagtttg ccttaacttt gttttcttag 42360
taaaaattag atttgccaag atagtactag gtatatttga agctaaaact cagaaaaaaa 42420
ctgattccta tggttacttt tgccagagag ctgtagtgat ttgtctaata tttcaaaatg 42480
attatagaag cagttctaaa gattatattt gctaagtctt ggaaacatgc acatttttct 42540
tacacattga tccagtacct agtagtttgg ttctttccca tcattagctt ataataccga 42600
caaaataaaa ttgtgaccaa tgcacatttg ctttctcaca atgtttctgg tatttttaga 42660
gaagttaata actaccatta tcaccaaagt gcagttgact tcaattatgt gacaatgtat 42720
ttatcaaaat agtgactcct cctgtgagaa tgaactacat gaattcttga gtttgaaaaa 42780
taatatactg agtttcctgg tcaaatagaa ttactcaaag taaaatatgg atgagaattt 42840
aaaagtgcat atttttaaca aacaaatgca tgagctatgt ggtttcattc caagtgtttc 42900
agtagtcaaa taaacagaga gagttgttca aacataaact tatttgtttt gaattttctt 42960
tcaattcaaa gtaaagcaat ttaaagcaag cataattaca tatgaaaaat cctaccaaaa 43020
aggtagcata ggcaataatt tgaattgcat atttccagtt gcctaagttt tctatgtgaa 43080
aagtataaag aaagttgaat agtttatgca gaaaagataa agccatgagt cccttcattc 43140
atttactgat tcagtcaatg ttaagaaagt atttatgtgt aagacactgt tttcagcaca 43200
caaaaacatg caaatacaca taaaatatga ccccatgcaa tggtggagac tgcacctggg 43260
aacaattaga aaaaaagaca ctaaatctag cctaggtatg aggaatcaat cagttcagga 43320
accccttcga acctaaggtg aatattgagt gcatcgtagt agactgtacc tggagctgac 43380
cagcctcatg tcaaggggaa gcaagaacac ggaggacagc acaagaaatt gcactaggta 43440
aggaaaatgc tttcagggaa gtgtaagaag ttctgtgttg ctaaagctta aaatatcagc 43500
aaagattggc taggagactg ggaagttagc tgggagggcc ttacttacaa agatgctaga 43560
aaatacagtt ctttatggga aattttaaag caaaaagaag ttgtagatat atagaaaaaa 43620
aggataatgc actgcttctg aatttctgag tggagatagt atagatttga gttaaacaga 43680
gtgacaagtc attccagaat ttttttaggt aaccaatagg aaatcgttca caagtttaag 43740
atataagtgc tgccacatat attgttacat ctttttatta attactaatt gaagtccctg 43800
tttggcagaa agtatagact ttcctccttg aagttccccc tatggttaca agaattgaac 43860
aatagtgtgt atatatgtat gcatgcatct ctatctatct atctgtctgt ctatctatct 43920
atctatctat ctatctatct atctatctat ctatctatct ctacctgtct atttctttcc 43980
ctccctattt atctcttttt ctctctatca catcatatgt ccagcaacac caatggtttt 44040
tctgtctaaa aacaatcatt tctaattcta aatgtggaat gaaagccaaa agacagatat 44100
ctgcaaaatc caacctgttt attagcgctt ttgcttcagt tgatacactc agggctgccc 44160
ttaaggatct ataccctgga aagagcagta aacagcactg atgctcttgc agcgtcccaa 44220
aatgagagat cttttggttc atgctccaat ggccatcctt tttttctaag cattctccca 44280
accaggaggg agggattcag aggagctaca acctgtgcag aaaccagcca tgtactatgg 44340
ctttcaaaca cagccatatg ctatgcagac accagccacc tatgcttcga cctttgtgta 44400
gatcttgtag aaccagctct gaatccctcc ctccttgtga gaccttcatt aagtaccttg 44460
gtatcatttt ttttcttttt tctaccagca gctaagttga ggctatccct ctagattctc 44520
ctaactaaaa atacttagga agtcaaaatt ggaatctcag tgacatcact tccgggaggt 44580
cctaagatcc tcatgctcac ctatttgtct ccgggtcaat gaatactaaa ggacatccta 44640
attttcaaat gtctatttta cacttctctc agtgtattcg ttgcttggtt gtgaaaatac 44700
acagaaagca taagaattct aaaaagctac tgactttggc tcaaacttga tcaccataac 44760
cactactaac aattacaata atgaatataa ttttactgtt agtagtgtga agaagacaca 44820
aattcagata caaacaattt aaaggagaaa agagattaca tcttcaaaac ttcttcacat 44880
tttgcacata acatcatcta atcacaggag tgacatcaac atatccaact caaaaaaaag 44940
gagttacaca ggatattata cattaacatt acagggtgag tctattagag gctctcttag 45000
aattctgcgt actgcaatat ctacatcatt atagcttcat atctatctat ctgtctatct 45060
atgttttaag tattaccagt tgattttatt ctagaagaaa ataggtaata tgcagctact 45120
tccacctctc ccctttctct cccattctaa ttgctgtaat tacttttaat tcatcatagt 45180
ttatcatttt tcactttatt cttgaggtaa aatgatgcag ttgtttaatc ttctatattc 45240
aaatgaaatt agtgcttact gtcagcattt ttatcattta ttctctgctt ttaacttctt 45300
tgtcttgatc tcttggttgg ctggatataa acatcaagaa gtttatgtat tttaaatata 45360
tattaaattt ggaagagcta atatttcatt ttgccttctt aactggataa cattttgctt 45420
ggttagaatg tattcttggt cagatttggt tctcttctga attttataga cagtaatcta 45480
atttctctgg cactgaaagt tgcaggggag tccaaagcta gcaagatatt tatccctcgt 45540
gggtgacttg ctctattttt actgatactt aaactagtct ttctttatca ttgatgttca 45600
aaaacttaac cagaatatgc tttagtgcca attattttat gttttttctt tttctaacac 45660
agaagaaaat gtgtgaattc attttctcct tggttccaga aacaattgcc tgtattatat 45720
tcagaatagt tctgccacat ttgttggata ctttatatta aggatgccaa ggattcttag 45780
accatactgt ttttatcttc cgaagctatc atctctgtag ttgtgagaat cactattttt 45840
ttctttattg ccattatctc agttttcaac atcatgacat taatgaaggt tttggctatc 45900
ttattctggt ttctatatgt tattaatgta tttattatat tttaataatt gctggttttt 45960
tcaaatattt tatggtttat gaaataattt ttaaaatagt gttttggaat gtgttttatt 46020
gaattttcat taagtttttt aatcatttaa agtattcata gggaaactat ttctctacca 46080
tgtaccaggg gttacatttt cttcaagatt ttggtcattt ttaaacagaa tttttatgtg 46140
tatgtgttgt atgtcttgtg tttatttatt catgtgtgtg tatgatgttt gcatgtgtat 46200
cagaactttc ctttttatat agttgttgac attgaacaag attctctttg ctgaacatcc 46260
ttgtttctgc cctggtacac ccaaacttta gttggtcttt cccactctgg acttgcatct 46320
tgcactttac tagcatcttg ggagttgatt tcttacctcc attcttttct ctgaggaaat 46380
agcagtgggt gtagccagag agcctcagaa accactttca gttcttgcta atggacattt 46440
attcttgtct ttcttctggg gtctgaacat cctgtgccag tgcccattcc aagcagtgaa 46500
gagtgcattc cgtccacttc caccacgggt ttattctccc ctggcagcac agccaacctc 46560
tggggtggtc ttgttataga agtgggtggg tctactttga tgattcttct tggttttcca 46620
cacggtgcct atctgcctga tatggtttgg ctgtgtcccc acccaaatct catcttgaat 46680
tgtactccca taattcccac atgttgtggg agggacccgg tgggagataa tttgattcat 46740
gggggcggtt tctcccatac tgttctcgtg gtagtgaata agtctcgtga gatctgacgg 46800
ttttatctgg ggtttccgct tttgcatctt cctcattttc tcttgctgtc accatgtaag 46860
aagtgttttt tgcctcctgc catgattctg aggcctcctc agccatgtgg aactgtaagt 46920
ccaattaaac ctctttttct tcccagtctc aggtatgtcg ttataagcag cgtgaaaacg 46980
aactaatata caaccccttt ccatccctta tgtattctcc ttccccagaa ctaaaactct 47040
aaataaaaat tttaatattt ttctctttcc tggactgttt ctggagtttt taaagctatt 47100
ataaaaagta ccagcctttt ttttttcttt tttcaacccc tgttttttac aagattgtgg 47160
ttagttgctc acttttctgc aggtgtgttg gtgttccttc ttccttccta tcttttacta 47220
cctttgattc gtttcactgg gttttggagc ctgctatact ccaccctctt caatggtcag 47280
tttccatggt gagagacaga actagctgga tttcctaggc cgactaagaa ttcttaacct 47340
tagctgggaa atgtgaccgc acctaccttt aaacacgggg cttgtaactc agctcacacc 47400
tgaccaatca ggtagtaaag agggctcact aaaatacaaa ttagactaaa agcaggaggt 47460
aaagaaatag tcaaatcata taccgcctga gtgcacaggg ggaaggacaa tgattgggat 47520
ataaactcag gcattccagc agggagtggc aacccccttt gggtcccctc ccttgtaggg 47580
gagctctgtt ttcactctat taaatctttc aactgcacat tcttctggtc cgtgtttgtt 47640
acggctcggg ctgagctttc cctcgccgtt caccactgct gttcgccgcc atcccagacc 47700
cactgttgac ttccacaccc ccggatccgg cagggtgtcc gctgtgctcc tgatccagtg 47760
aggcgcccac tgccgctccc gttcgggcta gaggctcacc attgttcatg tgcggctaag 47820
tgtccgggtt cgtcctaatc cagctgaaca ggagttgctg ggttccacag ttctcgtcca 47880
tgacccacgg cttctaatag agctataaca ctcacctcat ggcccaacgt tccattcctt 47940
ggaatccatg aggccaagac ccccagttca gagaataaaa ggcttgctgc catcttggga 48000
gccgcctgcc ccatcttggg agctctaaga acaaagaccc gcctataaca atggtatgct 48060
cttcattttc aaagtgctcc aacaactctt gctgaggtac aggtgagttg gcgaatcttt 48120
caaatcactt tcttgctaac cagccaagga ctctcattta gggccctatg gcctgttgat 48180
ttgtgcttat tatatttttg aagtttttaa tatttcttac catcttatcc acacctcttc 48240
ctgcactaca ctgaactccc ataatataag ctcgattaag gcaacaagta agtgtagttc 48300
tgttcactta gcagagctta ccacagaata ggcacccaat aagcatatgc taaaggaatg 48360
aatgaataaa tgcatccctt gtgaatcatt atcatccaaa gtacatatgg tattatgtta 48420
taaaaataaa ttaaagaata atagtttttt gttaattggc ttatgtaaga aaggttataa 48480
attgtcttgc attttcttat actaaatgga atgtgaagtt actactaaga gtgtctttcc 48540
agaatttgaa taagcagttg tgatactaat ccacattatc tcttcaagac acctgacagt 48600
aattacctcc gtgataaatt tgaagaaaga aaattgcttt gttatcttga gtttaagaaa 48660
ctgataaaga attaaatgaa tttaaatgga ggatggattt tctaacaccc tgacactttg 48720
accactgaag tgctcatacc atgtgaggga tctgtggcct gaaagcaaga aagcttttat 48780
tagtgatcct ttaaacaata taaggttttt aaccctatcc ctttctacct aatgacattt 48840
ttactaccac aaatgagagg ggccgaattg ctcagctcat gtttgatgac atttatattt 48900
acttaaattt atttatatat ttatttaata ggtgtgtcaa gcacctatta gtataagaaa 48960
atgtgctaaa acaccaataa ttttaaaaga tgggcaatgc atagcccttt ctctcaaaat 49020
atgtggctta aaagaaggca tcaattttaa gtaattcaag gtagaacaaa ataaacacca 49080
taagagagtt caaagaggga gggtgattct aaggacgaaa cagtcacatt agggaagcac 49140
tgtggtttag taaaaaacat actcagactt tggaatcaga gaggcctgat tctcctgttt 49200
atgacataaa catatgtggc cttggtaagt ctcagttttc ccatgtataa catgagagca 49260
cctatatgag cctgttgaca ggggtttttc agccaacata gtcttgtact ttattctttt 49320
gactccaaaa atggtaaaga gaaagaagat ctatctaaca tatcattggt ccacattctc 49380
tcagagtgat cattctaaat ccaaagctga aaatgccatt gttttccttt gatacctttc 49440
atagttacac atcttcttct gctaaagctc aaataattta agataatgaa caaatctctc 49500
catcatccag tacttgagtg cctcttgaat catgtctaca gccactcttt gttatgtcct 49560
ccggataaaa gatgtactag cctgtttgca gttcttcaga cacatcatga gccttccatg 49620
cttgcatatg ttatgccttc tgttgaaaaa gctaccccaa tttagcaacc agcaaatgtt 49680
gtatcctcca tagattagtt tagaactttt ttgttaaatt ctgagctttt ctcttccccc 49740
atcaaagcat ccactcctca ctcctatcta tatcactccc attagcatat acatactttc 49800
attttagcat gtatattgtc atttattaca tttattaatt tagtatctgt cttttttcta 49860
gattcataat acgtatgtgc tagcaatata tttattgata tgttctttgt cccatgagac 49920
tgtaagattt aacaagtaag gttttatttc tagcttaact ttaaatttcc aaagagccta 49980
atttcttttt gaaaaaagaa agaaacaatc aatcaaccaa tcagttaatc catgtagcaa 50040
ataggtaaca tattgaatat gcttctgctc atctagaata gacagtgtac ctccaaatac 50100
aatcatgagc ctaaagaggg gtgaatggat actatggttt ggctgtgtcc ccacccaaat 50160
ctcatcttga attgtagttc ccacaatttc cacgtgtcat gggagagtcc cagtgggagg 50220
taattgaatc atgagagcag gtctttccca tgcttttgtt gtgatagtaa ataagtctca 50280
tgagatctga tggttttata aagaggagtt cctctgcaca tgctttctct cttgtctgcc 50340
gccatgtaag atgtttcttg ctcttctaac atgcttatga ggcctcccca gccatgtgga 50400
actgtgagcc aattaaacct ctttccttta taagttaccc agtcttgagt atgtctttat 50460
tagcagtgtg agtacagact aacacaatgg acaaatattt catagtgacc cactgaccat 50520
tgatatcatt tggatctgtg tccccactca aatatcatgt tcagttgtaa tcctcaatgt 50580
tggagctggg gcctaatagt aggtgattgg atcatggggg tggattgttc atgaatgaat 50640
ggtttagcac catccctttg gtgctgtttt cgtaatagag ttctcatgag atctggttgc 50700
tttaaagtgt gtggcatctt tcccttctct ctgttcctcc tgctccagcc atgtaagatg 50760
tgcctgcttc cctttgcctt ccaccatgat tggaagcttc ctgagacctc cccagaaaca 50820
gaggccacta tacttcctgt acaccctgca gaattatgac ctaattaaaa ctcttttctt 50880
tataaatttt ttggtcccag gtatttattt atagcaatgc aaaaactgac taacaaccat 50940
catcttgagt ttccagactt gtgcttgtaa ggatgcctct cagctggtgt acataaagag 51000
ctatcaagaa ctctacctca agagtcacct tgcaatagac agaaatattt ttccttaatg 51060
tcaaaattat tctttactgc catccaatac aatcacctca ccagcaacaa caaaatgtgt 51120
ttgcacatct atgtgtttta ggatagaaga cataggaaaa gcacaatttg agatgaagtt 51180
gcagagtcag tattagaact atattccagg aatagagtca gtaaattctt taatgtgctt 51240
ctatactgag aattatttct cttccttcta ttgactgtaa taaaagaata ataagtctgg 51300
aagcacaaga gcaaggatac caacacatat aagattaact gttggtacca ttagaggccc 51360
cagcacagat taatgcctgg gtgttataac acaatcagcg gtagagagga agtttttcaa 51420
atcaaagcac tccatttgtt ttcataaaca ctgattcact ttaaaaattg atcagaaaac 51480
agattggtca tgaatgtcat ttaagattca gaggaaaaca cagtaccaaa aaccaagcaa 51540
agagaagagc caataaacct tattacattt agatgtcctt tctaaatctt ttcctccctc 51600
tctcccttgc ccctggttcc tttttctctc tctctcccaa ctagttatgg tgagaattca 51660
gcattgccat gatgccattc taaataaaca ttgacataag aatgagtctt gttgccaata 51720
ctctaatgat caacacaaga aaccaccgat taaatcggta gatacacagg tgttaatccc 51780
cagagccaag tccaactctg catgttcacc aacattgaga taagtgtgtt tcttgagata 51840
ttactgctca aatgaattta gcaagtgcca atagaacgtt tttttccatt ctaacaaaac 51900
tttattaaca tagtaggtct tgaaaaattt aatatgcagg ttttctctat atgaaggaaa 51960
taacagaaac attagatatt gcaatatttc ctaattttca cgtaccaatt atttcatttt 52020
agataacttt tgtaacactg tataatattc attttgtcaa cataaggtgt tgaagtgtca 52080
aaagtattaa gacacaaaag tttctcaaat atttctccca catgtaaagt ttctggttct 52140
gaaatcaaat atatttcaga agtgtggggt tttaatccta tgcttttctg gcacatattt 52200
tgcttcttgt ttccaagttt acataaaacg attattagga actataattc tgaactatat 52260
tgttcactta tgaaatgtat tgattattct tgcttatgca tactgaaaca tccatttaat 52320
tgttcaaaaa ctgtgaaaga tattttaaaa tgtttatttc tatttgaatt atagaagcaa 52380
aaaatatttg tcattaaaag ttatttaaga aaacttttat ttaggactca tcctaatgaa 52440
atccatttaa aatggcagtt atttaaaaat agatacctta gttcaggtaa aaagattttt 52500
cttctcattt caaaatgcta tagatggcaa tgagtcaaaa agagtcaaga taacaaactg 52560
ttctttgcgg cattagagaa gaagaaactc cccttcatga tattaagaaa gtcattaagt 52620
taattaatat tcagtacata aaaggactga acagatttaa atgcattttg tggcctcatg 52680
attttaccaa cacttagtca aaaatatgca acacagtgaa tttgaaaagt ccccccttcc 52740
tcctcctatt ttttcttttt ccttttttta aatctctttt atttttcaca aaagaataat 52800
gataattaat aatattatga gtatcagtaa ttgagccttt attttttacc gcacactctg 52860
ctaggcttct cactggtatc gatgcttctc ctaccaatgc ttcaagtcag gtcactgtgc 52920
tggatttctt ttctttttcc ctctagatct actacttttt tttttaattc ccctctgggc 52980
atcacctggc tgacccctaa ggtctacaac atcccttgct ctttgggttc cagttggtct 53040
cacagtggga agctctgttg agagtacaga gagcaagagg tgagtgagaa aatggccatt 53100
gatttcttct ctctctctct cttttctttg ttccctgtgt gggctgtgtt cctctactga 53160
gagcactagc tcccattagg tgggaatctc cctacaccca ccttctccaa ggcagtgaca 53220
atgcttaaca cttaccccaa actctttaac agctttatat tctttcattg gtcctgggcc 53280
tcattcctcc tgtcttctct cagcatcttt gccttcctaa attcatcctg tgctgtggtt 53340
tctttctcca aacttcttta gtggatctta gtgatacaga cagttatcac aaatatctcc 53400
tttcttattt tgaaatgtgt agcatatatc ctgtctgcca cttgaaacca aagcaacagc 53460
cctttttttt tacatttaac atatttaatg cagattgaaa tctgcacaag ggatcatgta 53520
accttcttca gctatctact tttagatctc aaaacttctg atttgacact tacatcctca 53580
tctccccttc accatgcact cttagagatt aacctgtgta ttcagctaaa agtgattaaa 53640
gtgattaaag taggttcttg tatttcaccg caaggttatt gaataaattt tgttttacat 53700
ataaattata ttttaggtat ttattaccag gatcaaattt tgttttggga aagtttgatg 53760
tctgtgtttt aatctcatgc caaaagcaaa taaaatatcg tggaaattat tttgatatta 53820
cttttactgt taattgcaaa tggaaatcaa agacaattgg aaaaaaattt ggctataaat 53880
atatattcct atatcaagaa tttaaaatat aaacttataa gccagcccta ccattttcac 53940
cttcataagc ttttcttttt gtctattttt gatctattta attagagttt tggagggaag 54000
agcagttgga agccttgaaa cttgaaactt tctttttcta gaaaaaacac tgatttatat 54060
aatcttaaaa attcactccc agctcctcgg gaagctgagg caggagaatg gcgtgagcct 54120
gggaggcgga gcttgcagtg agctgagatc gcgccactgc actccagcct gggtgacaga 54180
gcaagactct gtctcaaaaa aaaaaaaaaa aaaaaaaaaa aaaattccca gttcatcact 54240
ggccatcagg tagaagggag tgtggtggag ataatttaaa tgtaaaagtt taggtgactt 54300
gattttgtga tgattaatcc tagaaggctt acttggcttt cttatagcct ttgactttta 54360
gaaaaacata tagtaacatt gatgcaggat ttttcttgga ccctttgcca gactcacagt 54420
agggttgccc tctctactcg gaccccggca ctcaacccct tgcaggaggg agcatgtgag 54480
tgagctggtg tggaattcag caggccactc cagcacctac acagcaacga gctccatgca 54540
gggcctgtgg ccagcccagg tatgtcacct caaggggaat gtggtggcac ctagctgacg 54600
gtgcctgcaa ccctgaagcc ccagaggggg tgttacagtg ttcctttagt tccactgttt 54660
gcagatggtg gtgtgttagc agcttaattg gccccttgct tcattgtgtg gggcagctcc 54720
cctctgttgg caagggcaaa aggctggtgt gacagccttt ctgggtaccc gcactcggtg 54780
ggtcccaagc tcttgtctgg tgtccaagaa gaatgaggtc acacagacgg ttgaaggatg 54840
gtgaaggtgg aaaattttat tgagcaatga aaatggccct cagtggagag gggagctgga 54900
gaggggatgg gaagggcagg tcatcttccc tgaagtcagg ttgtctcttc cctgaactca 54960
ggtcatcttc tcctctactg actgagtcca gggtctttat aggcacagga aggggagtgc 55020
atgctgattg gtttgtgagt atgcaaaaaa ggttcaaata aaaagtatca ctcaaaggtg 55080
ggcacaacag tgtagaaaac caattaggaa agggaaggta tatgtaaaat aggcgaaggg 55140
tgggcatcaa tcaggggaaa acatgccaag caggaagaca agttctcagt ccattctgaa 55200
gatttaactt gtattttggc tttcaggctt taaactatct tcagcttggt ggtggggttt 55260
cactgggtac ccacccctat ctacctaggc atttggctgc ctcctgtcac tatcaatatc 55320
ttattgatac taaatattat tttcacatgt agttcttaga taaaatgtat gtaaaatagt 55380
gtgcttgaac ctgttgtgtt agcaacatgc taggagcaag tttagcctta caagctttac 55440
aaaaataagt ttgttcgttg gatttaatat agcacagaac tgtatttgaa agcagtagtc 55500
agaaaggaac acaacccagg gagaagtctg gctcttatca caaaatatgc atttcttgaa 55560
ccaatccatc atccaaaaat ggaaagtaga gacaataata aacaagaagg cttgatggcc 55620
atttccaagt tcaagtttct attacttgat tgcaattgag tgggcattac tgtttctcca 55680
gattattaag caagaacaaa attattatat tattatttct gaatttttga gtggtgactg 55740
gataaacaag aggcattagg aaatagagtt gcttttcttt ttttaattta catttatgct 55800
ggtagtagat aacataattt gttggtatat tgttatttat tttaaataac catattcata 55860
ttcagaaagc atgtgaggag taccttgtat tgaacattta ttaaaagcag aatgttgtca 55920
aaatttaggg gatttaattg taccatcaaa ttgtactccc aaataaatat tacagtacaa 55980
aataaaccca tggataatga aagtaaatac agcaaaaggg gtaagaatga atgggtaaat 56040
ttcatggaat tttaggaatc catgaataca taggcactta ttaattgtta tttgtataac 56100
aaattcttaa tgaaagtgct ttaacaacaa tattttaaaa gactgcattt gttttactgt 56160
gtaccaaatt acttccagtt ttagtggcct gaaattacag ttatttatta tttcacagct 56220
tctatagata tggctttgtt gggtatctct agctccgagt ctctcataaa gttgcaatga 56280
gattgccagc caaggctgca gtcatctcaa tgcttgacca gagaaggatc aacttgcaag 56340
ctcactccca tgggccacca cacagagcat acaacatgac agatggcttc ctcagctaga 56400
gggtcttaag aggcagcaaa tgagaggaag tgacaagaca gaagccacag tcattttata 56460
acctacactt ggacatgaca tcccatcact tttgtagcat tctattcata ttctatttat 56520
tagaaatgag ttaccaggta gagtgaggtg aagtgattaa acaaggcaca gataccagaa 56580
ggcagagatc actggggatc atcttacagg cttcctgcca gaaaaagatt aacacaaaat 56640
gatgcactgt gtgaaattac tatactagtc ttggaacact aaattttgat aatggagatt 56700
caatcaagca acacttttaa agcattaaat ggctaatcat aatcattcta aactcagaat 56760
ctttacgtta tttagacatt aaagtccagg ttaaaaaaag aaaacaacaa aaggacatta 56820
ttgagcatta ttgagttaaa ttgctcacgc ccccacagtc actctgtgga ccaagaacta 56880
tggctgggct tattttagga tgggaactga agagtaagag tgtaagtttt cagataaaat 56940
aggaccctta aacaattatg ctcccacact ggtttaactg gatcttaaca agtttaactg 57000
gatcttgtgt gttagcgcgt aggtgtttat aagcataata gtgctcccca agggaaaggt 57060
aaatttgcca tcacttccct accaaaatgg cctggtactt tcttacctgc cctaaagtca 57120
tgcttctttt caaactttga attatggaat gccagaggcc acaaagcaca acttatttct 57180
ttcaaattgt aaatctaaac ctcctaggaa aaaaaaatgg gcattaattt cattcgtgaa 57240
catatcccat aatgagtcta taacccagtt attttgtcct tccaacttta ttatgatgtt 57300
attctatttt gttttgcact aatgtcaaga agcattttat taaatcaaag ccaagatttc 57360
tagttttctt ttaaattttt atggggtgca tgccatactt tgatacagat atacaatgca 57420
tacgaatcac accagggtaa gtatggtaag gaattctagt tgtaatatta agcccaaatt 57480
atttctgcct caataagagt gacaattaga taatttctca tttttttcaa aaaactgaaa 57540
atcctataga catgtctttc ctttttgaaa agaaaaaaat aggggtagtc atcttatatt 57600
gattatattg taatattctg atttgttttt cagagcaata aggttatatt tgatgaagag 57660
ttttcactgt ttttttttaa aagaaatggt ggatgctttt gtgacttaat tatttttcag 57720
atgttcatgt tccatttagc taagacataa catgatcatg ctcagaactc ctgctgtttt 57780
ggcaggaagt ggaataaaaa acaaagtttt actaacttaa aacagtttct cttggccttt 57840
ctgttgactt attttatttc ttgaattatt ttattttgtt gacatcttga aaaggagtga 57900
ttctgaatta actgaattta aaaagatata ttcattcatt tttaccttgt gaaaccttcc 57960
ctgtatggat gaaaggtaga agagaagaca aaaaaatagt acattcagag actgactagt 58020
tatcttatgt gaaacgtaaa atcaaatctt gttaaacacc ttgcttggtc ttccaatggc 58080
cttcctttac atttagaatt aaattcagtg ccttcatcat ccatccggtt ctatgtgtcc 58140
taatctatgt cttctgttcc aacattgtgc tttgctattg tggctcaagc cactcaggtc 58200
tgtgtttttt tttcttccaa cccatgaatc tcattctacc gcttaggact tttatgctca 58260
ctgctcccct gtctagaaaa ctatgactca ttctttctcc tcattcagtc tctaattaga 58320
acagctttcc actgtgacct aatctatggt ctaaatctcc actaaccaca gtagctggga 58380
ttacagatgt gcaccaccat acctggctaa tttttgtact tttagtacag acagggtttc 58440
accatatcaa ccaggttggt cttgaactcc tggcctcatg tgatctgcct gccttgacct 58500
cccaaagtgc tggtattata ggtgtgagcc accatgccag gaccaagcta tgttttcttg 58560
accttctttc ttttcccttc catgcccata tggaaagtaa acaaataatc cactgcttca 58620
atatggtttt gctttgtttc tattgaatta cactctgttg tttttataga acatatcact 58680
atttggaaat taatgtttgc ttgggtttgt gtagttattt ttcagtttat catgtgcctc 58740
tcttgctgga atgtaagcta caatcatata gggatcttga aaacctgtac tttaaaaaaa 58800
taatttcaac ttttatttta gattcggtgg atactggtgc acgtttgtta catgagcata 58860
tggtactgga ggtcctagcc agagcaatca ggcaagagaa agaaataaaa gtcatccaag 58920
cagaaagaga agttgaactg tctctctgct tgtaacagaa aacccgaaag actctgccaa 58980
aaggctctta gaaccgataa atgccttcta aagtttcagg atacaaaatc aatgttaaaa 59040
aatcggtagc atttctatac atcaataatg tttaagctga gagtccaatc aataacacaa 59100
tcccatttac ataagtcaca aaaataacaa aatacctagg aatacagcta tccaaggagg 59160
tgaaaggtca ttacagggaa aactacaaaa cactgctgta cttttaaagc aagcagtaag 59220
caaggctgca ctttggaggt gtgaggtgtg gagagatagt gcctcatctc tcaaggttgt 59280
tcactgcaag tacaatcctg agaatggcca tggcccaaaa acacaaagaa gcaggagata 59340
ttgatggcat gtctccaaac actgtgttaa ctcccttcgc tgaggaagaa gagaaaatgt 59400
ttctgttgaa aaatgtcttt ttctacttcc tatgtaatgc ctctaatcca ataattaaca 59460
taattttata aattatattt tttgtgtttc aagattatag acttcggttt aagaatctgg 59520
tttttcatag ctctttttgt gagaaaaata cacacacaca cacatctcca cattacaatg 59580
tgtgtgtgta tgtgtgtgta aacatttatt tggtagctta ggcataaagt atggtttttc 59640
cttggcatcc tcgggggact gcttccagga ccccctgtgg atgccaaaat tgaagattgc 59700
ttaagtctca tataaagcga cataatattt gcatataacc tatgcacatt ctcccgtata 59760
ctttaaatta aattatgtct agattaccta tagtgcctaa tctgtaaaca gttgttatgc 59820
tgtattgatt tttttatttt tatttttaat tattgttgtt tttaaatttt attcttattt 59880
ttctatttta ttttattatt attatttttt tgagatgtag tcttgctctg tcgcccaggc 59940
tcgagtgcag tggtatgatt cagctcactg caacctctgc ctcccgggtt caagcgattc 60000
tcgtgcctca gcctcccgag tagctgggat tacaggcgct cgccaccacg cctggctaat 60060
tttttgtatt tttagtagag atggggtttc accgccaccg cacccggccg ttgtttattt 60120
ttattttttt gctaattttg atccatggtt ggttgaatcc atgaatgtgg aagctttgga 60180
tatggaaggt ggattgtact gcagtttgaa atatagctaa atttttagaa ggattttaat 60240
ataaaattct aaagcagaac tataatgcag gagactgctc atgtcctgat agaatcattt 60300
tcaggttata taatctgttc aaaatacttg ttttccaata aaaggaagtt gttttccata 60360
atcggttttt tggcagaagt ctcatttatt gtagtaatct gtacaggtat ttatttattt 60420
atttatttat ttatttattt ttgagacaga gtctcgctct gtcgcccaga ctggagtgca 60480
gtggcgcgat ctccgctcac tgcaagctcc gcctcccggg ttcacgccat tctcctgcct 60540
cagcctcccg agtagctgag actacaggcg cccgccacca cgcccggctg attttttgta 60600
tttctagtag agacggggtt tcaccgtgtt agccaggatg gtctctatct cctcacctcg 60660
tgatccgcct gcctcggcct cccaaagtgc tggattacag gtgtgagcca ccgggccgtc 60720
ctttattttt aataaatatt atcctctgct attatcaaat atttgggtga gcactctaaa 60780
agtataaatt gttaaatgaa acagagtatt tgatattact ttttccatat tactagaact 60840
tataaaaaga ggttagaaat ttttttgtgt gtatccttat attactttct aattttcttt 60900
tctttttttt tttttctttt gagatggagt ttctttccct cttgtcaccc aggctggagg 60960
gcagtggcgt gatctcctgg cttactgcaa cctccaactc ctgggtctat gcgagtctcc 61020
tgcctcagcc tcccaagtag ctgggattat aggcatgcgc caccacgcct ggctaatttt 61080
tgtattttta gtagagttgg ggtttcacca tgttggccag gatggtctca atttcttcac 61140
ctcatgatcc gcctgcctcg gcctcccaaa gtgctgggat tataggcgtg agccactgcg 61200
cacggcccac tttctaattt tctcttggca tatcatagtt gtataatcac attatacagc 61260
acataatatc tagagcctgt catagtttca aatttcaaac attgccatat attagttctg 61320
tcaccttggt caatttatat aattatgcct ccattttttc atctataaaa tgtaggtttc 61380
ttatgtgtta ttgcaagaaa taaacaaatg aatatatgta gaatgtttag aacagtgcca 61440
ggcacaaaaa taagaaacgt ataaatgtta gctagtatta ttatagcaag caaattgtaa 61500
agtccacatt tttttttttt tttttttgag aaggagtctc actcttgtca cccaggcaag 61560
tgcagtggca cgatcttggc tcactgcaat ctccacctcc aggattcaag caattctcct 61620
gtctcagcct cccaagtagc tgggactaca ggtgcctgcc attacacctg gctaattttt 61680
gttttttttt tttttagtag agacagggtt tcacgatgtt gatcaggcta gttttgaact 61740
cctgacctcc agtgatccac ccaccttggc ctcccaaagt gctgggatta caggcctgag 61800
ccacctcatc cagacataaa gtccactttt catgttctca cttgaatctc cttaagtttc 61860
tgatacagag ttcacacagt aggtcgagag caaaggctgt tggatatttt acaaaagaac 61920
actaaataat ccattttaaa agatgtttac cagttgggtc ttaccagatc tccatggagt 61980
gcataaatgt tatttgaaaa gctagaaggc tcttccaaaa tttgagcaat atactctacc 62040
caaggacaaa atgaaaaatt attaaacagg tatttgatag ctaaattggg ataaagtctt 62100
tataagaaca gtaagaggtt tgcatctaat tatgctcatt taaaaatcca ttacttaacc 62160
attaattaga caaaaagaga agaaagagta tgacttcatt tttatctaat taataggaat 62220
tttggaatgc gcagttccag aaaagcttat ctcctagagt gttatagtca tcactttaaa 62280
attaattatt tttaagtcaa agtgatgaga agtgcactac aaaaaattca atgtttgttt 62340
tgactaaaga aaatattatg aatataataa tataatatta aaaacaaacc aaatacacta 62400
actagcattt agaatgagtg aactttcatt atttcccagt gtatataatg gcaaacttgc 62460
acagtcactt gattttatag aaaaaaaaga atataaagag ttctgctaat ttcatgtgcc 62520
atcgcttatt ttatctgcat taatttaaag atatagtctg cctgtgccca ataaaaacat 62580
ctgtattgca aaatttaata atttatattc atatattcgt atagttgtac ttgtctttga 62640
tgaatattat cactgaaaaa aattgttgag tgatctgttt ctttcccttt tctttttgtt 62700
ttctctttct cacacacaaa cacagaacac tcaatcacat gtttaaaaaa aatgaatcag 62760
tcttttctaa tattttccct tatctattta atcctttgtt taaaaaatgg ctataaattt 62820
catgtattta aaagtaaaca gacttccatt ttacatattc agcctgcatt tattgaaaag 62880
ggaacaaaga aagaaaggta tcacgtagtt gcatgctgtc aggtaacatt tttataataa 62940
ttcatgtgat tccttttgta catatctatt cttgagttga ttgtattcag gcgaacctga 63000
gtcacttctg ttaatgtcac aatgagggct gagtccatta attctacttc actacacact 63060
gagtctgtga tagggataca actcctcttt ctagctcatc ttttagtaaa cttttgttat 63120
tcaattatga cacataaata aaatgaacag atcttaaatt acaccttgat acattttaca 63180
aaatgaacaa attctttaac cactaaaatc aaaatagaga atattgtcag tactccagag 63240
tccttccttc ttgccctgtc cctcactaaa gtgactatta ttttgacttc tgtcatcaga 63300
gattagtttg ttcttttatt tgaaatttat ataaataaaa tcttacagta cacacatttt 63360
tgtatctggc ttctttttcc caaaatcatg tctgtgagat tcatccatgt tacatgtaac 63420
attactatag aattctcatc cctgtatgat attctattgt atgaatacat cacaatttat 63480
tcattttact atagatgaac acttgcattg ttttcagttt catgatatta tgaataactt 63540
gctacaaata tttttagaca tcagttggag cacatgtgtg ttatatatgt aaatagaacc 63600
tctggatctt agagtataaa catgttcaaa ttcattagac tatcaatttt cccaaattgg 63660
ctgtactagt ttttaaattc caccagcagt atatgaaaat tgtagttgct acactatctt 63720
gacaatatta tatattgtca cttattttta atttttgcta ttatggctta attaatattg 63780
gatttttatt ttcctaacaa ctaataatgt acatctttta taagcatatt ggcatctttt 63840
tatgtagttt ctgttcatat ttagtaaagt gctcactcaa tattctccct ttttctaaaa 63900
aattgagttt tttatattct taaatgaaat aatgtataag aagtatgtct cttttaccta 63960
tatatcatat attttatatt actcatatat agatacatat tctctcaacc tatggcttcc 64020
atgtttactt ttaatactga gttttagtta agagaagaac ttaattttaa aagtctaatc 64080
tcacaattct ctcttttatg gttagtgctt tccctcttct atttaagaaa ctcttgtaca 64140
actccagaat cataatttat ttatattata gaatttcttt taacatttct aaactgtaat 64200
gtggaaacat tttcgtgttc attaatctac ataaatatag aacataaata tataagtgat 64260
ctggtgcaat atattgaaaa gaccacattt cccttactgc accttcccta gtatgatatc 64320
ttgctgtaaa taagttaatt ctattcatat gcatctgttt ctggattttc taatctattc 64380
cattggttta ttttttcttt ccttctggta gtacaacaat gtattaagtc ttaatatgtc 64440
ttgttatcct gcaggatgaa tcttccaact ctcttcttcc tcaggattct ctacccactt 64500
tcaaagtcct tcaatataat actgaagaga tgtggtgata aatgacatcc tactctagtt 64560
tttgatctca ggatgtaagt ttttgaaact gcagcattag catgatgtat acttgtagaa 64620
ttttgaaaat aacctttatc agattaaaaa acttcacttt tgcttctaag tagaccaggg 64680
cttctactga aacaataaga atagctgaat aaattacaaa agttgtattt ctaaaggaat 64740
tagaggacag aagaaataat gaggattaaa gtgactgaaa ctccagagag aatagagctg 64800
ttcaaaggtg aatgaagatg tgcactggcc ttttccaggt tgcatttgcc cattcagaat 64860
tggcagaagt ggagaatact ggcttcgccc aggcattagt catttcctgg ggaataagaa 64920
gtcagtagag gccgggtgca gtggctcaca cctgtaatcc cagcactttg ggaggctgag 64980
gtgggcagat catgaggtca ggagttcgag accagcctga ccaacatggt gaaaccccct 65040
gtctactaaa aatacaaaaa ttatccaggc gtggtggcat gcgcctataa tcccagctac 65100
ttgggaggct gaggcaagag aatcccttga acccgggagg cagaggttgc agtgagccga 65160
gatcgcacca ctgcactcta ggtgacagaa tgaaattctg tctcaaaaaa agaaaaaaaa 65220
aatccactga aactctgtct caaaaaaaaa aaaaaagtca gtagaagttt tagtagttgt 65280
gcaaagatac agtgacaaaa tcagagactt aatggggtct aaaacacaga gttggttttc 65340
tcccaaagac gtgcctcacg ttctgaagct tcctgtagca agagactaaa aaaaaataaa 65400
gcaaaagatt ttggaaaaca cagcaaaact cctacagtct tggagtgctg gagaaacaga 65460
cacattcccc aaactctcac ccaaaatcct caaacagcca agtgttaagg atgaagggta 65520
agccacaagg agactgagtc ctaaggactg caaccaactt tacccatgct caggcctcga 65580
ttggattaag tgatcaattt ccaaacctct tctggtgaaa gttacatcat ctaatgcctg 65640
tattttttat agtaaataaa cagcatgcaa ttaataatta ttctgcttgt gcaataggag 65700
catatgccaa tatcacaaag aaacaaacaa aaatacaagc agattctgtt tttcaggtta 65760
gcagataaga acttaagata cttaagatta atatacaaaa gaaaaagaag aaaaggaaaa 65820
aatattgact gaaaagtgag gaatttcagt ggaaaattag aatctataaa tagatttaga 65880
acaacagtct agaaacaaaa atgtgaccta tgaacttaag aactcaactg ataggttaac 65940
aatggatcag acacagatga taagagttct gaactggatc aatgtaaaat atacaaactg 66000
aagcacagag aaaaagaaaa agaaaagcac ataagagaca tatagaacat acttataatg 66060
tgttaaataa cctgtaatta gagtctcaga atgaggtggg gaatgtaaga gggggttagc 66120
attattcaga gaaaaaagtg gctgaacata ttatattatc tttatgttgt ttcttaaggc 66180
gtaatagtga aatagtatga agtattaaat gatgacctta aaaaaaatct tttatctgaa 66240
atattccaac acagttttaa atgtgagata gatctgagtt tactgcctca aaaatttata 66300
atcagaaaca ttttaagcca aacagcatct ctaggatgat ttatctgaaa cccctttgtt 66360
ttatttctga ggtcacaaag ggattgaaag acttgctaaa ggttctagag cagataacgc 66420
ctcaactttc catcttattc tatttatgga gacactttcc gtgaacctga ttactaatat 66480
gacccaattg cagtagctgg gggtggggga acaagtaaat atttccttgt ttactctgta 66540
aagcattcag ttatgcacta aaaataagaa agaggatact ttgggaaata attctctgac 66600
tgttctagtt tatctaagtg aacctaaata aaacaattat tacgatatta taaattggct 66660
ctgggaatct ccagctctgt tttttgttcc attagatatg taccatctta ccaagtttcc 66720
tgatcctgtg aatttcttgt aagttctacc tcatttctca ttctgtttat gtattgactt 66780
acagtcctga acacccaact ctttatctaa tattgtgttg tctgttattc cttctactaa 66840
aagtcagaat gtgaatcctg gctctctgcc ccttatcagc catgcaattt gggcaaatta 66900
tctgcttctg agccttgttt tctttatatg taaaattagc ataataaata taacacctgc 66960
atagttgaag ctcaatttat atcattgtgt gtttcttaaa cattaaaatt ctactgttta 67020
ttaatcataa aattaattta agaatgagat aagtgaatag tttctaccat aggcaggtgt 67080
taagccaatt acattaaaca aaattctaga aataaagtac tttgatgagg gctcagcaga 67140
tacggtccat aggccaaatc ctgttcacca cctattttta taaataaagt tttattggaa 67200
cacaaccata tccatttgct tacataataa atatggctgc tttgctgtta aaaaggcaac 67260
tatatttgtg tagttgcaat cgactgggta actcacaaaa cctgaaatat ttattagcta 67320
gccttttaca gaaaaacagt tcactgacct catcttaaag cattgcctgg cataacaata 67380
cacattaatt gtgtattgtc aaccaaccct tttcaaaata atacaaaatc cagacttcat 67440
tatagttctt aaatacttac tgataagttt gattggtatt taggaaatcc acgccaattt 67500
ttatgaattc tacattaaaa gagcattttt tgctagtatg ctttcctttt tctgtgggtg 67560
aaaacaaatt tctggtgtat tatttttaaa tttaagagta gtaattatgc acagctgaag 67620
taaatacatt ttgcataata attaatgatt tatagagtct actaaggcta gtttgagcta 67680
ttggatataa tgtgcatttt ttctcttaac aatcctcata aatcaagatt tactcatatt 67740
aaataatact aaaaaagaaa ctcatttctc ttttgctgtt gtatacaaga acattaaaca 67800
tataactttc atttaatttc agatagttaa tataaaacat tgcagaggaa atggatatct 67860
attttggaaa gatggaggca attgttgtct atggcaagac agagaaaaca catttctttc 67920
tacttgaaac taagattatt tataaaagga aaactaccta ggagatgatg actgggtaaa 67980
gcttgtctaa ccagagccat ggcttcttcc tcatgctatt cctggaaacc accttttaaa 68040
attgttccca taagatgttg gatcataccc aatgtgaaac catcaaaaga taattgttat 68100
aatagcaaca ataataagtg ccataatgag tcactagaca tctgtattta catgctgagg 68160
gattatgtct tagtttctat atccaaagtt agggttgtaa ttattataca taagttacca 68220
ttatgcccag gtaggacatt taatatacac tcaataaata attatttatt gaataacaaa 68280
cagtttacct tgcattataa ggaaatctga ctagatgaga aggaatttta gtaactatag 68340
aaatcagtaa tgattctaac atgatcaaaa cgtcagcata tgtggaacat gaaagttaga 68400
agttttattg ctctatgata tagtaaaaca aactatggaa caaacgctaa tggtgaaaaa 68460
taaagaaaag ctatgagaaa cagtatatag aaaaaaatca tcagaagttg atgattaatt 68520
cagtgtgaag taatggatta aggaaaatga aatcaaggaa gtcctcattc ccactgtatg 68580
aaaaaatcat atttaggaat aatttcaata aagaattatg gatattgaga tgatggataa 68640
ttccatgata catttagttt taatgaagaa aagattatgt caagctaaat cttagaattt 68700
ggaagaaggg gaaaaaagta aaacagaaat ggccaggttc caaagacata aaacagtgcg 68760
ttgttggaac tgaagaaaat agttccaaga agttccatac aaatactaaa cgatcaaaaa 68820
gccctgaaaa aaaatgacac tatatttgat agtttgtatg ccacagtcaa ccttgttaat 68880
atcattttgg taaaataagg ggagtggaaa ccttgctatg gagccagcgt tctgacacat 68940
ggaaatgaag gcaattaggg caaacatatg tttgagaaat tggggtgtca aataaaatga 69000
atagtaactc tagagagtca cagcacgagg agtaatattt tcttttacaa ggtagaagag 69060
gccaaaggtt ttcctactta agacagaaat agcataaaga atgagcttgc atgtacaaga 69120
aagactgaga aaataagtag gaaggagttt aaaaaggggg atggaatctt cagcaatcat 69180
ttgattcttt gtctctttgc tcacctaatc ctctaagtta aagctatttc accttcatct 69240
atggctcaaa gtcaataata attgtatatt ttaaaataaa gaatgtgatt ggattgtttg 69300
taactcaaag gataaatgct tgaggggatg gatagcccat tctccatgat gtgcttattt 69360
cacattgcat gtctgtatca aaacatctca tttaccccac aaataaataa acatatttac 69420
atttatttat atatttatat aatatataaa atattagtga caatgaataa taataatata 69480
taaaatattt attatatatt tattatatat tcatttataa atatatatat ttatacatat 69540
gtatgtactc agtacccaca caaatttaaa aaataataaa aaatctaaaa aggaaaccca 69600
tatcaggatt caaaactaat attttcttga gagaggacac atatcaaaga caaagtcacc 69660
cttagaagtg tcaggagctc tttgctctgc aattacacat gtatacactg cccagtagca 69720
atggctaaga taagcaaatt gaaaatggag accagtttag ctttccagat cctttgtgag 69780
tctgtttgac ctaaggatgt ctaatagcat catggaaata agaccagagg ctcctagtca 69840
ttttggcacg agggaccagt ttcatggaag acagtttaac cacagaccag gtgttgggga 69900
ggttgtggat gattcaaatg cattacattt gttgtgcact ttatttctat tattattaca 69960
ttgtaatata taataaaaca gttgtgcaac tcaccataat gtagaatcag tgggagccct 70020
gagcttgttt tcctgcaact agatggtccc atctgggggt gatgggagac agtgacagat 70080
catcaggcat tggatactca taaggagcgt gcaacctaga tccctttcgt gtgcagttca 70140
tagtagggtt tgcgctcctg tgagaatcta atgctgctgc tgatctgtca ggaggtggag 70200
ctcaggtggt aatgcaagca atggggagtg gctgtaaata cagatgaagc tttgcttgct 70260
tgcccaccac tcatctcctg ctgtgtggcc cagttcttaa catgccacca tggaccagca 70320
ctagtctgtg gcctaggggt tgggaactcc tgaattagac aatgaggcct ctgaaaaaca 70380
agattctagg ataaattttg ataaggttat ataattcctt cttggtgatt tccctttcaa 70440
ttagcttgcc taatactatt ccatcttatg gaatggaggc ctactaaata tctaacagtc 70500
ccattttgga ggttttatca ttgagaattg tatccagtga tcttattttt ggttaacaat 70560
ggacaccttt tgataaggac cagataagtt ggaaagcaaa accaacaaac aaaatgaagt 70620
tgtcaatcaa acaagcgaaa taaaggatac ttctgtgtgg ttcagatatc tttgtgtcaa 70680
gacatcattg agcaaagggg atttgtgtcc tcagttgaag aaagtagaaa attaaatcct 70740
aagaatccaa atttaactaa ggatatctac tgttttaaaa cccattgctc tcacaaaatt 70800
gcaatcataa aacaagaaga aaataaaact gtggtgtaac agagctacaa aacatcagta 70860
aggaaccaag caaataaaaa agacatgtaa tattttcgca gacttcagtc ctatgtctct 70920
attgcctgga taattggtat gaatgtgcca gtcagtcaaa gcatacatag catttggtgc 70980
aatgacgtct cccatttgta acataaaaat aagattgtta ttttcataac agatcaccac 71040
attgtactct tttaattatt agacttaaag cttatattga tctctttgat tcctgatcca 71100
gaagagcctt tgcaaaccaa gactactcgc tcctgtgggg catcagtttt tcaggttgat 71160
aagagttgca gtaccttgag gaatttccag tgctattggc ttcacccctt cttcaagcta 71220
ctgacattct tgtccttcat tggtacttac gacattgttt gaccaagtgg aaggcccata 71280
tagttgttaa attcacttct cttcacatct gagccatttc aggtattttt tttttttttt 71340
tttttgagat ggagtctcgc tctgtcgccc aggccagagt gcagtggcgc gatctcggct 71400
cactgcaagc tccgcctcct gggttcacgc cattctcctg cctcagcctc ccgagtagct 71460
gggactacag gcgcccacca ctacgaccgg ctaattattt ttgtattttt agtagagacg 71520
gggtttcacc gtgttagcca ggatggtctc gatctcctga cctcgtgatc cgcccgtctt 71580
ggcctcccaa agtgctggga ttacaggtgt gagccactgc gcctggccca ggccttttta 71640
tatgctgaaa aactaaacag tggaagcaac cctatctgta tgctactcct gcctggaact 71700
catgagcaac ttccttacct gctaaaaaag agggtggctg tgtctgaggc aaaagtcatt 71760
gttgtttgtt taattttaat tacaagcaaa attaagtcta aacatgagac ttagaagcca 71820
aaccaaatcg aagctattgg aattggcgca ctattctgcc tgccacatgg cagggtgcag 71880
tctcaacaac ccttccgtgc cttacatcct taagaactag aatcttgggt ctggctggca 71940
ttttgaagaa ggcacatcca agcagaagag aagcacatta ttgaaatcag agaagcctca 72000
gagccattcc actatgataa tttactgtct gcacttttaa aaggctaatt tagaagtgga 72060
gtaagctcct agttttatgt ttacacactg ccctttttcg ttattgcagc tgaccctttc 72120
tcaactcgat gatgaatctg agattcttat acctctttcc aaataatttt aagagacaaa 72180
actcatcttc aactgaaggg aataaataac tcagagatgg cattggaatc cttactaggg 72240
gggcttaggt caggaaaggc aaagtttttc tatggtgtgt gcctcaaaca taagcctctg 72300
gctagagtca ggacttagta atcataagag tccttttcag cctctctcct aacttcacat 72360
attagtgcat gcgtttgtta caaccaaacc agactctact gtttcaggat gcagttcatt 72420
ctaaagcttt tttccaaagg ggatatccaa atctcccaac aggtgcgttc aaaagacctc 72480
cagtccatac tcagatggct ttgacctatt tccaccctcc gagtctaacc actaccctct 72540
tcagtaccat tcacacctca aggacattat ccgggtagac tccccttatt tagtgtatat 72600
ggactggtgt tctccacagc acacagagaa cttccgatga attggtctct tcagttacaa 72660
ttcagtctct ctctactgaa gcctctggaa tagtcagtga cttaggatat gaacataaac 72720
actgctctgt cccagcttga ggtctgggtt cagatcttgc attgggtgac tgaaggttcc 72780
agcctttgct tcctgattaa ctagaatctc tcccagccag gtgtggtggc tcatgcctgt 72840
aatcgcagca cattgggagc cgaggcaggt ggatcatttg agctcaggag tttaagacca 72900
gcctgggcaa catagtgaaa cactgtctct acaaaaaaaa aaaaaaaaaa agaaagaaag 72960
aaaaattagc tgggtgtgat ggcgtgaacc tgtagtcgca gctacttggg gggctgaggc 73020
aagaggattc cctaagtcca ggaggttgcc atcacaccac tgcacaccag cctgggtgac 73080
agagtgagac cctgtctcaa aaaataaaaa aatctgtcaa tgcttggttc ttacctagtg 73140
ctctaaatag gttcttgtta acattaccaa agaaggtagg aataagaaga aatttatttt 73200
cagaatatga attgatgaaa aaattcttaa tcacagttgt cttcagaata aaaaaaataa 73260
gggattgtta ttttacaatt tagtaaatat tccccatgtg tttcagtatg ttaaaagaat 73320
tcagtaaata tcacaattca attaaatcag cccaggtttt atggcggtga cccctcatga 73380
ctggccaatt ttaaggcaac ttgattaata ttcttttatt gattcacaga aaaggaaatc 73440
attcattggt ccctgtgggc ataccctata aagcttctgg gcgtgagaca gtgtgagcaa 73500
caggttgtcc ctgccgctcc ctggatccaa acaggtcttt cagagttgac agaatagtta 73560
ttagagacgg cctttccttt ccttgattgc cttcaactga atgttctaaa gatatagaat 73620
gtggtattca cttattctca ttaattcatc actttcaagc ttaaactctt ttattaaaga 73680
aaaaaaactg aggaccaggt caaactgcaa ttctggtgtt tatttgaaac aaaagtcatg 73740
aaacaatagg cttataaaaa tgaaaggatc gtgagaattt tagccattct cttcccctca 73800
aaaatgagct gtgtattatc tatgtaggat ggtcatctag aatttgtgta ggtacttaat 73860
ttcatcattc atttattcaa caaacatttg ttgatctcta taaaatgtca ggtggtgttc 73920
tgtgcccttg tagtcatcag tgaacaaaat agccaaagat tactgccttt ttttgaagta 73980
tacattctagttggcagtgt cagacaagca aaaatatata taataaaaag ataattatat 74040
cttattttag gaaaatagtt ctatagaaat aaaaggaaga aaggcagagc caagttaaaa 74100
aaaaaaagat tgaaagtgct agagttaggt ggatagacat ggttccatgt tacagaagtt 74160
ggtcagggca agcttaattg ggaatggtta agtaaagtct agaaggaggt aagagagaaa 74220
gccttgagac tacctgggaa aattgctttt caaagctagt aggtgccata cgatggagct 74280
atgtctgatg tgtgtgagga aagacaaagt tttagtgagg gaatgagagc acagatgggg 74340
aggtcaggta ggtcacagag gcttttgggg gtactacagg tcactgtaaa caaacttagg 74400
gtttttgctt tgttttgtaa cttttacttt aagttcaagg gttcaagtgc gggtttgtta 74460
catagatacg cttgtgttat ggtggtttgt tgtacagatt atttcatcac ccaggtatta 74520
agcccagtac acattagtta tttttcctga tcctctccct cctcccctcc tctaccctct 74580
gatgggcccc actgtaaatg tttgttggat tttgctggta acatacatat atgctgcata 74640
tcttctttta agtatcaaat agtacatcat cgaaaataaa atataattta acctgctgtt 74700
ttatagtatt tcctttctta tccaaaactt taatgctgtg catgaccaag gtcttttgat 74760
aatagcatat tctcatgtga atcagtaaaa ctggatcaca atgaacagtt ttggaaattc 74820
ttggtaagtt ggcctcttag ccttgaatat gcctatccta atcccttctg tcgtgttaga 74880
ggaacttttt ttttgtttct tcttttcgtg agtagtaata gacttgttcc tactggttaa 74940
atgcctgagc tttctttttt gtaccataaa tcataggaca gtacatcttc acattatctg 75000
gaaaaacaca ctaagaaaaa aacaaaaaca catgcatttc ctccatgccc ttgaatactt 75060
tgcaaggtcc ctggaataac agtactctct gcttccctgt ttagtcacat aaggaaacct 75120
atagatggca ccactgctgc cagtagccat cttgctgtaa tacgagccaa acttcagaaa 75180
aagggcagtg aaatggactt agaatcccag taaactttgt atcaaaccaa acttagagct 75240
cactctgtct ttaaattttt caggtgtaag taaaatgttt gcttattttt aaagcttctt 75300
tgaattaaga atattaatac aggcaacata tgtacttcta atgcaatatc taagaacaat 75360
tgcctccaac aaaaaaggca taaaatgtat ttctcaagaa atgtcacttt ttttagcaag 75420
aacatcagct tcagcttgca ggaagttaat tgaaaatccc catagataga aatgactgaa 75480
gtgaatgccc ttcaggtcaa taaagcattg ctcagttcct taggtctttt gataaacttg 75540
cagttcattt ggcttcccct gctgttgttc attaataatc actgaagctt aataattatt 75600
tcctaatgct tatcacttat ttttccaccc cactttatta ctgtctcaca ttagttacct 75660
tgctcccatt gtgcgtgtgt gtgcatatat gtgtatttat tttccaatag catggtttta 75720
ttttgcaatt tttttatctt ttaaaatgaa gatgggaaaa ctctaatata taatttcaat 75780
gtatttaaaa atgaattatt ttggactgta atgtgaaagt aaatgtagtc ataaccgaaa 75840
gcaattttga aagttctaaa tgaatcctta ttttctttta aaaacattaa attacagtgg 75900
ttggatgtgt ctgatgagct tcccccttac ctttcaaaat gtttgctttg gatataaata 75960
tatttttata actcagttaa tttgcatata cattcaattt tcaccccaat ccaaaatcca 76020
aacccctgct atttttctgt ctgcacccca cctgtggtaa cactgtcacg ttgtgatttt 76080
caagcttaat ttctgtcttc aaaagcaaca gaagcagttt tgtgtttaag ccaacagagt 76140
cctagtttaa aatttctctc ttttctcatt tgcacatttt taatattcct tttatcgcat 76200
tccccccaca tacatcttga aaagaaataa aatatctcaa gtgattcaac tttaagtact 76260
gtatttgcca acaaggcatt gaaaacaaaa tactctcaaa ctgcaggata ttttataatc 76320
acaaatttgc tcattcccat tatccccaag gggctaaagc acatattatt attccattaa 76380
ggaaatgaag actctccgag ccaagcaatc ctctctttag ctccctcctc ccattcccat 76440
gttctaaaac tttctctgca taaatagttc tcaaactttg ttagctataa ccttgtgact 76500
tgcagaagat tcttaagggt cactcctgca gggattctta agaggtaatc caggttggtt 76560
ctgttttccc acttgaaaca aagcagctca gcctttgcat tgtatgttgt aggtctgtct 76620
catattgtac ttggaaaaat gaaaattaaa aaggaacttg gtgttcaaca tggctgaaca 76680
taatttttta attaagagtt tatccatgaa tacagtactc tagatttttc ctagaagcta 76740
cccaaatcaa cacaaaataa cgatgacaaa aatccaaaac agtcaaagca aaatttaaca 76800
tttatatttt tctctaaaaa tatttctata ctaaaataat tcatgaaaaa tattgcttgt 76860
atttaatata caaattcatt ctattatttt ttcaaaatga catatagtta ttttgaccta 76920
tagaatccat caggcaccaa gattggggta gatccctctt aaaattaatc tgttttatca 76980
tatacaattt atttatgaaa gttttcttct atttctactt gtttttgtat aagtgtgtgc 77040
taaattttat caaatgcttt ctctagatct attcatatgt ttatactttt ttctttatac 77100
tttaatgtgg aaaattacac tgagtttttt cttagtcttt tagataattc tgatgtaaag 77160
gggcatgact tttttccttg gagaaatgct cagtgcaact gacgttatga tttgacctgt 77220
tactttttga gattaattaa aaattatcat ttcccagagt atcatgaatg ttgttctaat 77280
tatttagaat aaatccaaag taattgcata tttataatgt gttgacaatg tttgaattaa 77340
aggcccagaa tagccttttc caagatgtgt tctgttgatt tctaacttca cagaatgctc 77400
aaattgactt tcatgcttat atacatttgg gaaacaacac acactagatc ttaggaatct 77460
taggatttac ttaggaaatc aaagtatatg ttagcagatt aaaggttctt ataagttctg 77520
tggaatacat ttattcatct tttttactga aggacctctt tctttgcaaa catttgacag 77580
cttttgaagc cattaacaca cttgcggaaa gttctgtctt ttgtagatga ggatttttga 77640
aaagctttat caagttagag tttattaact tgtgataatc tggagattta ccaaacctat 77700
gctttccttt aaaatatata taaaaatata atttctgatt ctataaagag tttaagacta 77760
tctatataag aagcaaagac atcattgaat gttcatatct aaataatagt taaacttcta 77820
tgtaggcaca ggtgtccctt ttaactatct cttatttttg caaaaacaaa tattttattt 77880
ctaactcttt aagcataagt taaattaaag ccccaaaaaa ccacttactg aagaggaatg 77940
aaaaataaat catgacaaaa aaagtaggct tttaaaattg caagtggcaa ttgcgcttta 78000
tacatgtaca aaagagttta aacttgctat accaaaacct catttacctg aagtacaata 78060
attctaaatt tttaacagct taacctgcta aaggaatctg gtgaaattaa cctttttaat 78120
atctgtgaaa gaggatatag aaatcaaatt aaaatcatgg aagcaataat tcagtgaata 78180
ccataatttc tttcttcttt tgtgactact taatatctta attaaaaatc tctcttatct 78240
atttgcaaaa gcatttacaa actctaacat tgagtagcct taccccaagc cagtcaatgt 78300
gcatgaactg gaagatgatt tgcagataga taacttttaa attgcttctt tattaactac 78360
aataacacaa ggtatctttc atttctttca tacgcaactt ctttctgagt cttccaattc 78420
tttgtcaccc ttctcctgag gtcagggaaa atatgcaagg tatttctgag gagaatggta 78480
tgactaagca aatctttaat ccataaagtt ttcttgatct tcttatgtaa taatggcata 78540
aaagaatgga gactaatatt tactgagcag ctaattggta taaacaccat gtcgggagct 78600
tcctatgcct tttaagcctt acagcaaacc tccaaacatc tccattttgc tgatgagcaa 78660
aagtctagaa aggtcattca gtaaataagt agtgggcttg aacatgaagt caaattcatc 78720
ttgctctcaa cgctatgttt tttctaccct gtcacttgct ttactgtatg aaaatgtctc 78780
gttttaaagt gtactttaac atttttggaa ttatggtcct tcgattaata cttgagatat 78840
tctctgcttc ctttacagtg gagaaaaatg aggttaatta tagaaaagac tcaacccttg 78900
atcattaatc cctagtaaga agaaaaggtg ggattagaat atatgctttt ttattccaca 78960
tgctggtgtt ttttgtttgt ttgtttgttt caatgtcttg cttctttagg catgcttgcc 79020
ctgttgtttc cattacccta gaaggccttt atgcatataa gcaaatctct ctattatgtt 79080
aaatcttcct acccacaata ttccgtagac taccttttgt tacaagccat ttcattggat 79140
tcagatcagt cactgaaaca tgatcctccc atcagtacct gaaactcatt taactcatga 79200
tgtgaacctg acgctaactt ttgaagaaat tcattgtggc cattggtacc ataaaaaata 79260
agagagtttc agcttaagtt ctaatgttct tatttattgt ttatgaaagt tatacccaat 79320
gtaaagcagg atgcttgaga aaaaaataga gccagaacag ttaaaattaa tacataacaa 79380
gatggtacaa ggcctctgag ttgagttaat caatgcagtt gataacccaa cagtaactgc 79440
actagcaagg tggacatttt aaaatgattt aattttaaaa actcagactt tttgttccct 79500
taacttccaa atcactgtat ttatattctt tcctattgtg atcccatttt atattttatt 79560
cttcttgagc ctgcaaaata ttacaataaa ttttgatgtt tactcctctt ttgcaaagat 79620
agcctaacaa ataattcttg atttattaaa aaacaacaaa ccaacaactg agaacaatac 79680
ttcattgtgc tttgtccttt ttgttgttgt tgttatgaag ggaatgtaaa gcactctgtt 79740
ttgaaatgaa tagatgaatg ataatgtgct cttcaatggg acactgtctc aaaacctatt 79800
ttatctccgt gactgggatc ttctatgtgc ctcagtcagc tggttgatca gcactaggtg 79860
ctgggacaag agccagcctg gcctaacaaa ggagtatgga attgccttga gctctcagga 79920
ccagaccaac catttttgta tcactcaaca tagatgaaca atacagaaac tgaccccaga 79980
ttcatcactt tacaaccctt tcaaaatatc caaataaata aaatgtctcc atttactcaa 80040
atgtttagtt ttaaaataca gattgtattg aaaaagtctg atatattatt ttctccatag 80100
tacatgttgc aaactaattt tttttagcaa aaatagctaa cacttagtga atatatactt 80160
tattctaagc actctccaag ctgccttctc acttaatctt cacatgaacc caattagtac 80220
atactattgt tattaaggtt gttaaaatgg gaaaagtaag gtgtggaaag gttaacatga 80280
catacctagg gtttcatggc taatatgagg atgagtcaga ttagaaactg gtaagtatgg 80340
tattacaacc tatcatttat tatttcatta acgtcgtcac gcttaccatg ctggctaaat 80400
taagttatct gaaagactat tttgttctta taagagtgtt ggcgctatta ggcaagaata 80460
ttttcttcct atgtaagact gagctggaaa aaggggattc agaactgtaa tcatcgagga 80520
aacttctttt cctaggttat agggaaagag gaacaaaaca ttgctggagt gtccctgtga 80580
gaacaagaga caacaagaca acaaagtgag acttgactta ctctcagaga cccccccttc 80640
cttggagtca gggccagcaa attcttagaa attaaaccgg ctatgtgctc tcatagcaca 80700
agcaagccaa atgacatggt agcagcaagc aaaaaagaaa tgtatttttt aattgtttat 80760
ttgtttaaat tttatttcaa tcaccttagg agtacaagtg gatttttggt tacatggatg 80820
agttgtggca tggtgaattc tgggctttaa gtgtacctgt cactcaaata gtgtatgttg 80880
tacccaatcg gtaattaaaa atgaaatata tttttaaaac attttccctg tgagaagctt 80940
gaataaatca aaacacaaac aattcgtgtc agaattgttc tctagcacaa aagggatttg 81000
ccagatatga aggtaggtca ctttctggag atgggacaaa gcctacatat atacacaaaa 81060
cctttcaaaa cttatgtgtg tatatatata tatatatatg tgtgtgtgtg tgtgtgtata 81120
tatgtatata tatgtataat gtatatatat aagtatatat gtgtgtatat gtacacctat 81180
atatgtatca gtatacatat atcagtatac atatatttat atagagttta tatatgatat 81240
atatactaat ataataatat actgatgtat tagtatatat catatataat atgtatacta 81300
atatattagt gtatatatct tatataatga ttatatgtta gtatatataa gtatatatta 81360
tatatattag tatatatatt aatatatatt attaattatc ttctgcaaac ctttctaagt 81420
tgctgcatgg attctgtaat tccggacttc ttttcccctt tactgtcaat cctaaggcaa 81480
caggaatact taacaacaac agttattttg aaaggatgct tcttggccct tctcgctaac 81540
tgcttaaatc attcctctgt gttaagaaat gaaaaaaaaa atccttactc aattcctggt 81600
ggtttctagt tgattgactt ctaatgagct atctggcatc agcagctcca tgaggctgaa 81660
aagatttttc ccattctctg atttaggcca gtcttgcgtt cagtttgaac atttttgcat 81720
agctcattca atgcacgaaa atgaatatag accacaaaga aatgttcttt aaaaactatg 81780
tcagagacaa agatgtctta tatcattttt aatccatcac tacaaataaa tataaatggg 81840
ctgggcgtgg tgactcatgc ctgtaatccc agcactttgg gaggccaagg caggaggatc 81900
acttgaggtc aggagtttga aaccagtctg gctcgtttca ccctgaaacc ccatctgtac 81960
caaaaaatac aaaaaattat ccaggcgtga tgatgcatgc ctgtagtccc agctactcag 82020
gaggctgagg catgagaatc acttgaaccc aggaagtgga ggccgaagtg agcagagatc 82080
atgccactgc actccagtct gggtggcagt gtgagaccct gtataaaaaa taattatgcg 82140
tgtgtgtgtg tgtgtgtgtg tgtgtgtata tatgtatatg tgtgtgtgtg tgtttgtgtg 82200
tgtgtggtaa ttaacaacat acataatact taaccaagcc ccttattttt tccactttta 82260
ccacacaagt tacttgaaga gaaattgttg tcatttttca tgatcatttc ctcaccatag 82320
ggagttagaa gcataaatag acatttgcaa taaccccatc ctcccaaacg tcctcttctc 82380
cagctcccaa gtttcttctt ttgctctttt cttcctgttc cctctttcca tttctcaatc 82440
tctgtttgag ttaacattag cttagaagag caatggattc tgagaccttc tacgcattct 82500
agttcgtttt gttaccaaat aagtacttaa ttcgttgaaa gaaagaatct gatttcatta 82560
atgaggaaat aaagtatgtg ttcaacccag gaacaagaaa agaacaaaaa atcagcaaga 82620
tggctataag ggtaggttat agaaccagac aggtggcttc aaatatgggc ccctttcctt 82680
tgctaatgac gtgaacttgg ttgacttgtt cacactcaaa agactcaatc ttttcatctg 82740
ccaaatggga ttcatgtcag ttttctagga aatcagcagg cttaataaat agcatccctc 82800
tttcccatcc atgttctaaa aatgttgaga aagtgtgaaa aaaagaagca gatggggtca 82860
aaattttcac cttgattcat gataactact aagtgagatg atgtagctta gtgtctgagc 82920
caaataaacc taccaagtaa atatgagact gagacactta accacctgca catgccagac 82980
cagacctgaa atgcaaagac cacaaaggtt acacatcggg catggaggta cgtcacctac 83040
tgactttttg atgggagagg aggagaagaa gaagtaaata aaagaaaaaa ggaagagctg 83100
aagtcagtac acccaaatta ttgtcctcaa atttactagt tcgatgaatc atcttattac 83160
ttctggtaaa atagtgatta aaggttagaa aaagacagaa gtgtttatat cgggggaaac 83220
ttcaggggct ggaaagaact attcccccat aattttcccc caggtttctg aggtcttaac 83280
cctttgtgtc tagacattgc actcatcatc agtcaataag ccgctaccca ctcagggaag 83340
gagagcagca ttttttttta caacagcctt gtgtccaggt tcttgtactt ttccaatacc 83400
agccatacct ccactgggtt gtctcctaga cctacaagtg gctgttttac attttgttcc 83460
cagaagactc tggaatgagt cacaataaaa tgttaaataa atggactcta aggtggtatt 83520
ttacccaaag ctgaaaattc ataccctgat ctctttctta acctcgaact atgtccagcc 83580
atattattca atgtagccta ctagggcact gcaaaaagtt tgaatgtaaa cctcatgagt 83640
caataataaa atttctccca actagggatt ccagaaagaa gttaattcaa gtagtctttt 83700
gcttgttaaa gtttgtttct tatatataat agtccaaaag aaccccccat cgaattcttt 83760
tttatttgca aattacagat aattttgcaa atgtaagatt atatatatat atatgtatat 83820
ggtcatgtgc cacataatga cgtttcagtc aaggatggac cacatgtatg aaagtagtcc 83880
cataagatta taatggagct gaaaaattgc tatagcctgc tgacttctta gcctttgtag 83940
tcatatcaca atgcattagt cacatgtttg tggtgatgct ggtataaaca caactattgc 84000
tctgccagtc ttacaaaagt ctagcacagg caactatgta tagtacataa tactagactg 84060
ttataataaa ttactatgtt actggtttat gtatttactg tgttatattt ttatggttat 84120
tttaaagtat actcctactt acaaaaaata tgttaactgt aaaacagcct caggcaggtc 84180
cttgtaggag ggattccaga agaaggcatt gttatcatag aagatgacag gttcatgcat 84240
gttattgccc ctgaagacag cgggacaaga ggtgaagaag aaaaacagtg atattgatga 84300
ccatgactgt gtgttgtctc aggctactgt gtgtgtttgt gttttagtct ttaacaaaaa 84360
tgttttaaaa ttaaaaaata aaataaaaag aagaaagtgt atagaataag tacataaaga 84420
agaaatattt tttgtacagc tgtacaatga gtgttttaaa ctaagcgtta ttacaaaaaa 84480
agtcaaaaag ttaaaaaacg tgagaaagtt tataaagtaa aaaagttaca gtaagctaag 84540
gttaatttat tgttgaagaa aaatatttta aatacattta gtgtatccta agtgtacagt 84600
gtttataaag tctaaagtag tatacagtaa tctcctagac cttcacattc actaccactc 84660
actcacagac tcacccaaag caacttccag tcctgtaagc tccattcatg gtataccagt 84720
gtgctataca ggtgtactat atttcatttt tcatcgagta tttttactgt accttttcta 84780
tatttagata cataaatacc attgtgttac aattactgac aatattcctt gaagtcatat 84840
acaggtttat agtctaggag caataggcta taccatacga cctaagtgta tagtagcatc 84900
taccatctag gtttgtgtaa gtacactcca tgatgttcgc acaacaacaa aatcacctta 84960
ggatgcattt ctcagaatgt atccccatca ttaagcactg catgactgta tgtgtatgta 85020
tatatatgta tttatttaca tacatatata ataaaatgga tcccaccctg ccttttttat 85080
tttcaacttt tattttaagt tcaggtgtat aagtgcaggt ttgttgcata ggtaaaattg 85140
tgtcacgggg gtttgttcta cagattattt catcacccag acactaagcc tagtactcat 85200
tactattttt cctgatcctc tccctcctct caccctccac cctctgatag gtcctagtgt 85260
gtattgttac tctctatgtg tctacatgtt ctcatcattt agctcccact tacaagtgag 85320
aatatgtggt atttggtttt ctgttcctgt gttagtttgc taaggataat ggcctccagc 85380
tccgtccatg tccctgcaaa tgatgtgatc tcatttcttt ttgtggctgc attgtattcc 85440
atggtgtatg tgtaccacat tttctttatc cagtctgtca ttcatgggca ttttggtaga 85500
ttctatgtct ttgctattgt aaatagtcct acaatgaaca tacatgtgct tgtgtcttta 85560
taaaagaatg acttatattc ctttgggtgt atacccagta ttgggattgc tgggtcaaat 85620
ggtatttctg tgtttaggtc tttgaggaat gaccgccctg tcttccacaa tgtcaaacct 85680
gcttcttact cttctctgac ttaactaaag ggcatgtgat gccaatatag agttagtttt 85740
ccttcagcag atgcaacctg ggctgttttc tcaggaatag gttcaatttt cttcaggact 85800
ttggaagtta aattagtttg acaacaagac tctggtgccc atgattttct ttttagagtc 85860
caatagaaat aaagccacat aattatcacc aagatattta gaaaataaca gacttactct 85920
gagaaacatt atattcctag aacctagcac ttggtactgc caaaagctgg agaagatatc 85980
ccatgttatc tgaacaaaag aagaatgcct ttggtttatc tttagccgag tgcctctcag 86040
ttcacaccag ttgcccagaa tctgcaatct gtaaccttgc tcagttggga ggaagctcct 86100
tgcagatggt tcccttggaa ctgtgcattt gctatcactc cttgttctct cagacatcac 86160
tgctttcttg gcgagttaaa atttttgcaa tggtgcagtc ccagctccaa gatcattttt 86220
ttgtgttatt ttttctttta tatccatatt ttcaaaatct gttcatttat acttacacct 86280
tgagtctcat gttcttgcca cccaagttcc aaattgagtc agcctttgca tttaccaatg 86340
tgaccctttc cactgcctcc tatttttgaa ttacttatgt ccaagacacc agtcttctac 86400
ggaaaatttt cactccagtc agagccacca taagaccaca tgttgggagg tggttttgga 86460
gtctgtaggc cagcgacagg agtgcaatct ttgttctttc tcgatttctt tgttgtctga 86520
tctacagcat ctacaattta aagattgaat cttattaatg cacatagaac tcagtctggc 86580
accaaaaact gtgacttaac cctcttacat ctaaaaggcc agtgcacaaa ctgtttttct 86640
tgtgacagat tttatgctat ttcaagcgtt acttctaggg taagctatag gaacttgcca 86700
ttgaggcttt ctgcagtgga taatgcaagt tggtgactgc tgagatccaa acacagcctg 86760
aatcttcacc atctatgtgg ccagcatttg ctgaggtcct tataaggctg ccatgactgc 86820
agagaagcta taacattgtt cgcatgcccc gcctgaggcc tgagttgcct acacatcttc 86880
agaagccctc ctctgctcaa ggtcagggcc ttgcatagag gttaagcctc attggcagag 86940
acatgtaaag cccgagaacc attcacttga agctactcat gcatacaccg ctgatattat 87000
gaaaatggct acttcactct ggaagaagcc acaataagca tcaccaaaca tttagcacag 87060
ctgattgaag ccttatggcc tgtccacagc agctaagagc caaggctcga gggaaaaaat 87120
ctgcctaggc tagtatacgc ctttacaaag caccaagata agaatgttct tcccacacac 87180
taaactcatg gccccaaacc atatttctta cccacatcaa aaaccttttc atggagatct 87240
tcagcttctc taattattct ctttgtcttt attgctcaat atttctgctt gagtctccgg 87300
ggatgtgtgg gaattgatac cctcattctc tgtatattct ctgatctaaa aagattgaga 87360
gatagaatat atcgggaagt cacaaatcac catgtgcaga tttccaatgg acacatcaaa 87420
catgtttcaa cacgataaat tctcttagcc tattttgaga atataaaaga gtccagagaa 87480
taaggagaaa gaaattttat gacaatgcat tctttggaga atactgcatg agtctcatct 87540
gatgccatct ccagcatgag cagttggccc atccacctca ccttaatcct tccagtggtt 87600
tccaaagtgt aatttggaga ggtgattccc atgcgtgagg ggcatagcca actgaaatcc 87660
tccctgtgcc tgataatcct ggagcatgag acatcttctg aatggtctat agagatgctt 87720
ctctatgggc attgccctgc ttttccagtt atttccaaga acaaagaagc acaagtgctt 87780
cccagggact ggatgtgggt ccttgggaga gctgatgaga gtcatctagt ccaacagaac 87840
acaccagatg gaggttggag ggagagggaa gaatgagtgg gaagagacag agtgaacatc 87900
tcagggctca ggaaggagca tgagcagtca gcacctcgag tggtgagaag ctggaggtgt 87960
tctactcatg cattatcttt cacatgaaca atagcgtcaa aagtgggcgg aaagcattct 88020
ccccacacag caatgcccat ttaataagtt tgccatgatg attgacttgg taatgcatgt 88080
aaaaggctta ggactgcatg cttattagcc tatcaccagt acctacgtgt ttaacattat 88140
atgacaaata ctcggtgagt gtcttagttc cactctttcc taatatttat tacacacccc 88200
ttctcccact cctggctagt cagcttgcat gtacagtcat ttctgctata atgcaacata 88260
tgtgttccta agaatcgctg ctctgtgtaa cgtcaagcaa ttaaaaccac agagctttgg 88320
ataaatcggc ttaggcatac aacactcatg atcagtgaca cattttttaa aaagatagaa 88380
atataataaa gatggcagca catcctgtat ctaagataaa agtggaaatt aaaacaaaaa 88440
tggtagcaca gttttatcca tgtgaagtgg ttaagaaaca catatcacat tcaatatggc 88500
acctggagtt tgcttgtgga agcggacagc tctgtgttat tgtgaagtga gggaaggagc 88560
atcctctgaa accagacaga aagctataac accagatgta gacgggtgtg gcttgtggtc 88620
ctcgtgaggt gtgtgcattt gtgtgctttg tgtatttcta ggtggctccc ttcagccgag 88680
tgcagttttc tgtgtttacc tactgcttct catggatgaa aactcatgta aacaaatgca 88740
aaatttgtgt tatcctcaaa ctgaggatga tataccaatt acgttggaac aaattcatat 88800
tttcaaaatg agcagaacag tacatattta caaaccagaa gccacacatc ccctagcctg 88860
ctttgaaaag ggataatttg taagaatgtg atctcccaag tgcaatagtt agaatctttg 88920
gatttaggcc acgagcgttt gccaaaagaa atatataatg gagaagagag ttctggagac 88980
ctttgttggg aaagccagaa catcctcata gagttactca actctggcta aattatatgt 89040
gaagacctag acgacaccta aagaattatt gtgcattttg aaagcaggaa gaggaaaaga 89100
aaaggaggaa acaaatgact ctgatcaatt cttggtaata acaaaggagg aaacaaatga 89160
ctctgatcaa ttcttggtaa taacaaagga gatacgattc ccactacact ctcacgtgat 89220
acattcaaat ggtaactact tttcgtctat ttttgccaaa aatgaagaca taggatcata 89280
ggaaaaaaac aatgtttact aaatctatca atcaaagcca ctaaatccta attcttctat 89340
ttcaagatcc attaaaaaga aatttctggg gccgggcgcg gtggctcacg cctgtaatcc 89400
cagccctttg ggaggctgag gatcacgagg cggatcacga ggatcaggag atggagacca 89460
tcctggctaa cacggtgaaa ccccgtctct actaaaaata caaaaaaaat tagccgggca 89520
tgggggcggg cgcctgtagt cccagctact tgggaggctg aggcaggaga atggcgtgaa 89580
cctgggaggc ggagcttgca gtgagccgag atcgcgccac tgcactctgg cctgggtgac 89640
agagcgagac tcagtcacaa aaaaaaaaaa aaaaaattct acttatttat ttttaacttt 89700
tattttgggt tcaggcatac atgtgcaggt ttgttatata ggtaaacttg tgtcatgagg 89760
gtttgttgta tagattattt tgtcacccag gtactaaacc tagtacccag tggttatttt 89820
ctgtctctct catctcaccc tccaccctca agtaggccct agtgtctgtt gttcgcctgt 89880
ttgcatccat gtgttctctt catttagctc ccacttttaa gtgagaacat tcggtgtttg 89940
gttttctgtt cctgtgttag tttgctaatg gcctccagct ccatccgtgt ttctgcaaaa 90000
gacattatct catttctttt tatggtggca tagttttcta tagtgtatat gcaccacatt 90060
ttttaatcca gtcttccact gatgggcatt taggttgatt ccatgtctat gctattgtaa 90120
atagtgctgc aatgaacata tgcatgcatg tgtctttatg gtatattcct ttgggtacat 90180
acacagtaat gggattgctg ggccaaatag taattctgtt ttagaaatgt ctacttcttt 90240
gtttttggag tgaacaataa caaataagtc aaatcaaaca agaatatata aaggtttgtt 90300
tgtattttaa ttattaaaat attgaaaaag gaatacatga attatttaca aaagcaaatt 90360
gtcagtcact ggaggcaggt gccaaaataa attcttctct tcagtgtaat aagaagtgct 90420
ctgaagcaaa ttggaatata atattgataa tgacaataaa ttgtaaattc gtataacttt 90480
gtaaaaattt aatatttgtg catttctgaa gttctttgaa ccttcttttt tatcagcagt 90540
gtttctaggt agtgttcatt tcttcagaca gaaggttctt aaagagtcat gactgcctta 90600
ttagttatgt ttgtgcttcc aaaacttaca gtccattcca tctgtggtga gccacttcag 90660
caaaatatat tttggaccat gaattattgg gcataatgta acaaagcaac tggttgtttg 90720
acattttttt tttgtttggt atcatgtaga tagttatttt tagaaccaac tctaaatgac 90780
ttttctccgg cctttttctt tttttttgag atggagtctc gctctgtcgc ccaggctgga 90840
gtgcagtggc acaatctcgg ctcactgcaa cctccacctc cagggttcaa gcgattctcc 90900
tgcctcagcc tcccgagtag ctgggactac aggcatgcgc caccacatcc agctaatatt 90960
ttgtattttt agtagagatg gggtttcacc gtgatagcca ggatggtctc gatctcctga 91020
cctcgtgatc tgcccccttg gcctcccaaa gtgttgggat tacaggcatg agtcaccgcg 91080
ctggccttct ccagcattca acaagtgtca tgctatagtc gacgtgtcag aaatatccag 91140
tgagaatcag ggaaacctca ttagaaagag ttcctgattg gttcccatat gttcctgagc 91200
tgtttactat attagagtat ggtttcagtt tctcttattt ctttcactaa tctttgggct 91260
ctaacaaggt atattctcat tataatctct ctgttctctc aaaatctaac acagtactaa 91320
gtacaaagtt agttctctct atattgtagt taaatggaat gagttcatta caagaagatt 91380
agggctgaat tagcccagtt tggcatttta catatttgaa taatcaaatg gtagctagaa 91440
cctctatttt gcttggtttt attaattcag tgggggacac ctgccggatt taatttctcc 91500
ctcatttcta atggactaag tcagtttagg tcactgtgaa gacgtgggtg aggtcatgaa 91560
cttcagtcta gggctgctgt tttattgttt agactatcgc agccctccta agccaaattt 91620
cttatatact ttcaaaataa aaatgcagca aactagtgtg gaagtagcct ccagagcgtt 91680
ctgcccctca ccacccccgg cccctccaaa gttagactta atcttcttgt ttccatgtca 91740
aatccaaact ggaccaccat cactagtttc ctaggaaacc ctgaaaaact gtctttacat 91800
aactcacttt gcccactatt ggaatgctgc cacttctggg gaaaggcaca gcagctgttc 91860
agctgcctca tagcaacact tcacaacagt gtgggaggaa agcaaagacc agcaatatac 91920
ttaaattaaa cttccctggg gagagcaaga taccagggca gaaggtaata gtttagagtc 91980
gagagcatct aattagtcaa caaacctgcc ctgaaagcga acgtagtgaa tttttaatga 92040
aatcaagaga cccagtgttt tttctcatct tccacttgaa aggcactgat acatatttaa 92100
ctgctatcat cgaaagagtc cttctgtttc aaacatactt ccaaagaagt atttttttta 92160
tttttatttt gaactaaaca acaaaaaaga acacccacag caaagaataa aggtcaactg 92220
ggttatgttt tcatataatt cctactattt ccccaaaagt taataacatt tcatccagct 92280
accccttgga ctagctagga ctagctagaa ttgccaaaac tcagatgaca atgcaagaag 92340
catcgtttca acctggctct gttctctctt tagcacggaa acaaaacaca tgtttatagc 92400
agccattctt tgaaccgtga ttcataaaat aggggtagaa attaaagagg taaagtcaaa 92460
gttgccaata gtaagaggaa aatatttgaa cggatggaac ccaaactgcc atgtaatgct 92520
gtgcagtttg ctgtgcataa aggcactcag ccaacgaagc aaatggaagc ttaaaatgca 92580
gtttgggctc ccctaaccaa actgtgcacc caggagtcag gctactactt cccgaagaag 92640
gggtaccttt ttctcagcac aaaggcgcca tctgttggcc atctcaacag aggggctgct 92700
ttctttcggc gggttgtggt cattcagagg ggaagccaat ttttctagtg gaaaccccgc 92760
atagaatggg ggaccctaag aggcattctt gtacaaatac ttcattttat acataagaga 92820
acaaacacca ggggtccagt agagaacaaa aataaggacc tctgtttcag aaagcagaaa 92880
aagatgttat taaaagtact aacagaaact tttttctctc tccatgaatt ctctgaaata 92940
gtcatggtgt ctttatttgc tccatgttat tgatttagtg taattctaag caaagacaat 93000
tttaaaatgt aattattagc attaatttcc cttttcatct ttatgtcgtg aaatgtcagc 93060
ttttaatatg aatgtaaatg cattttgttg gttacacgga gtcactgatg ctatccactt 93120
cctattttgt aggtcgtaca cgcctaccca tttccttctt atcagaagag tgaaaacact 93180
gcagaaaacc acctcttctg tttgtatttt acttccggaa acatatacat cccatcaacc 93240
ctctctatct tcagtttact agtgaataaa gaaggactta aaggtaaaga gaccatggct 93300
actcctatat tccaatgttg tcttcatttc cagcctaagt ggctggctgg cacatggagg 93360
gaagtaacag gagttagaat ggctatcata ggttccttgt tcctctatgt ttcccagaag 93420
gccattgtct tatttctcca ccggaaagca acttcaggtt tgaatggaat atgtggactc 93480
tagggatgtc agagtccctg ctcactccct tgggaatggt acatgcttac ctgtactctt 93540
gagtctttct agaccctcag gcattgtgag tctactagaa ttttgtgtca tgcagcattg 93600
caaatgctat aagacaatgg ggaagtacgg aactacaggg aacattcaaa gtgtgcatat 93660
ctcctctgct cacacatagg caccattgtc ccattagact ttacttacag aacacaagtt 93720
gaaggattaa attatcaaga atttcaagac agtaacatga gagcattaaa ctagacacag 93780
ggcccttctg aggactgagc acaggttgcc agcccacaaa actggacatg agaaatatta 93840
agtgatttgc ctaataacca gagataaatg cctttcaact tcagctcatt tctaagcatt 93900
aacttctatt tacttttttt tttagctaat aaactgacct ttctaacaat tcttcagtgt 93960
tactattaga tcaatgaata atggaatgca aatgtttggg agggaaggat gctaacagtg 94020
atcgaatgct catggtgctt tgccgtgctt acctaaatga tcttattcat tacaattcta 94080
atgaggcaag gtgtgtttaa tgcattttta aagctggata cttaaaacct taagaaagca 94140
agtaactcat ccaatgtcaa gtaacttatg tgttgagaaa gcaagatcaa tgagttataa 94200
acacacggaa cttgagttcc gttcccaacc tccctttcaa aatctattca gcggcgtcgc 94260
tttcccatga ctctagatgg agagttgggc gggcccttga gtttctacct gcttggaggt 94320
attgaacagt ttctcccatt actttagatg acaactcaga ttcaacttgg aagctgaagt 94380
gtgcaggtat ctgtaacatt cacatgtctg gattgactta atgttaaatt ttcttccctt 94440
ccctagctgg gctgttgaaa ttggccacac cattgccaac agtgacacag cacttcctct 94500
ttcctgcttg agtcttcctc cagttattag tttgaatggg agtggttagt acttagaaag 94560
tggggtgtgg agcatgaaat atgggagtgg ggatgagaaa tttcttattg gggtgagttt 94620
tgtgccaact cagacttctc ttaatctcaa ggattctgtt ttaacctggg ttctcacaat 94680
gcagaacctg cataatttat tgggaactgt aaccctgaaa gtgagggaca ggggtagtga 94740
aacaaggcag gttcaaaata aggatgtttc aagatggtca atgtcaggat atctggatgt 94800
tcactcctat gggatcttct ggaaagctga gtggcatgag ctttaggatc aggccttcag 94860
cagaggaagg agaagttttg cttgctggtt tctatgcata tttatccaga gttcatccca 94920
tgagacatta gccctcttag agttccagga tgaaaaagag tgatcacaaa attttctgcc 94980
atgtcacaga aaagcccagg gatgggccac aggaatgctg gatgggcttg atgtatggtg 95040
gtgccaagat gcacctgcag gaagccattt ggggtccatg cacaggccca tcactgaagc 95100
tgtggccaaa atcagagaac aaaggggttt agtggatctg atatgatgca tgaacaatgt 95160
ctaacatggg tgctttcctg ggttcactgg aggagattca acttgggccc tccaactcaa 95220
attctaggtg tgtgccctgc ctcctcttca gacatttgcc ttctgaaagc ctggcccttg 95280
ggaggctcac tttatagcac atatcacctc tcatatattg gagtcccatt ttcctcatcc 95340
tgatagtcct tttgagcagg acttcaatag ctccagtcca gttcactctg caacaaaagt 95400
ctgcattggg tcctagaaaa acagtgacat attccttggc ctatcatgga agggaagtac 95460
agttactcct gaaataaaca agaaactcac ccctgttcca ccagccaaag cagcttctat 95520
aactttctat ttttccctta agttttcctt ggctgaaagt cagaccgcaa tgcaccatat 95580
tgtcaaatga gaggacaaag cattcacaat taagtctcac tttaaagcct ctcttctttt 95640
cagtaaaaat cgctttgttt ggtgattgac tttatgatga taaacatacc tgaaaaagtc 95700
tgatggagag taagaaatac agcttttcta aacagaaata aggctttcag ggaatgttct 95760
acaggactat gagttcctta aaacactgca attcatttct ccctataggc tggctaaatt 95820
tactgtcctc tcagtgtgag aactagtctt ctcagactcc ttgaaaaatg ttccaatctt 95880
tactgttttt tagaaagtac ttggatacga ctagaaaata cccatccttt ttaagaattt 95940
ttaaaattct taaattgaaa acacctccat taattttagt aatgcagaac acaagataga 96000
actgagggtt gtgtttctat tcttactgta gtgtctagca aagttatata aaattatgca 96060
taatatatag tgattatctg aaaaaaataa tttgaaataa gcccaacatt tctctgcctt 96120
ttcttgatta agaaaatttg gaggtcaact aaaaagcaca cacttattaa gactttaact 96180
gaaatattgc tctgtggatc aggaaaaaag aaattccaag aaactcaaaa attcagagca 96240
tgaaaatata ctgatcttag tgaagactgt attagaatca gaaacagatg acaagggaag 96300
ggaggagaaa gtgggaacca acaattcagc atgagaatag aactgtgtct atagtcattc 96360
tgtgaattat taagttttca cctttggaaa gatgtcttcg caaggctagt agttttaagt 96420
acaaatgggg ataatgccaa atgatggttt tgcataaaaa gactggtata gcttagaaca 96480
tgcagttctg aataatattg gagtaggaaa gcaagtcaac aagctattgt gtgtgtatgt 96540
aatggactta attagaaaac atggatttgg aagaagttta cttcttccag atcattcttg 96600
gggctattaa tacgaaaact gaaagtcatt agtgaccagc gttgcctcaa atcttaccag 96660
gtcaacccca gctgttctgt tatttcatga gaaaaacatc aactgtttaa tcattttatt 96720
tgtattaata attttattac tttaatcatg atgaagaatt ctctaaatga tccttttgcc 96780
aaagatcatg ttcaccttca agcctaaact gcctacgaat tagatgtgct gttttcccag 96840
aaacaatgtt ttcattacta ttaataagta gttactttta ttaatcccag acaaatgaca 96900
gtttaaaaga catcatcaga ggatatttaa gattttgttt tgccttgttt tatttattca 96960
ctccaatgct aatgatctgg aggaaaacat tgtttcattg ttttattcct tcaacggtat 97020
tatattattt aagcaacaac cgcagcaaaa tcctcaggtt ttggaagagg cccattagct 97080
agcggcacta aagtgggaag aagtaattaa aattaataac tcaatgcaga ggattcctgc 97140
ttctgaaaac gattagcatc tctttcctgt tcgtactttt tcttctttgt gtgtgtggat 97200
agaaacacga tcatgcttgt tgcccagact ggggtacaat gacacgatca tagcttacta 97260
cagcctcaac ctcccaggct caagcgatcc tctcacctca acctctaaag tagctgatac 97320
cacagataaa tgccaccatg cccggctatt tatttttata ttttatagag atggggtttt 97380
accacgttgc ccagggtggt ctcaaactcc tgggctcaag caatcctgcc ttggcctccc 97440
aaagtgttgg gattacaggc atgagccacc ccgctcagcc tgtccttttc tttacagtga 97500
atttaagttt taaaagttgt gctaaattta tttgaacatt acatcctgca ggacatgtta 97560
ggatgcaccg tataagccat gaacgttcta atactgcagg gattcagtga actttccatt 97620
tccatacaaa caacctccta tcttctgtgc cttccttgag gttcccctaa ggtgccctct 97680
tctaacagga ggggtaaagc cacagtcagc ctgctaacag ctacacagca atcaggaaag 97740
tgagaaaatt aaactaccag tgacacatgt tccgtctcac ccgtttttgg agaattgatg 97800
gagtgtggta gttcaccttg tacctcttca cctttacagc acaacttgaa tgggtctggc 97860
ccattgctgg acagttggag gctcaacttc atacattatg ctattactct ctgtggaaca 97920
ggaaatttct tgttgctttt aaaactatct gtttgcttcc ctccatatag atgggagaat 97980
atggcttaat ctgccactgg gaaagcatta catcatggta gggctcaggg aatttgagag 98040
ccaattaaag ggtgtttttt tctctttaag attaaaattt taatattgaa cattactatt 98100
tttctaagaa aaacaactat aacttctaga atattcaaca cttttaatta taattaatgt 98160
cctatttatc tcatgcattg ttaaatctgc aatttacata tttaataaaa gcaacattat 98220
taggtatcca ttaaaatcta taaaatgcaa gtttccatta ttgagtaaat taatttctca 98280
gtgagtggct gtttctcttc tcccaaccac tgatgaagca acattattca tcagtttaat 98340
tttactagtt gataatacat tgctgcaaac attaattttc taatgcatcc ctgtacttac 98400
tttgtgtgta tgtttgagct ggttaagagg ccctgacgat tgttttaacc attgacagac 98460
tgcaagatga gctaaacttc agctttcaac gaagatagac tgagtctgct tgtattgaga 98520
tatctgactt tccttaatta caagactttt ccaactgctt cctaaaacgt tggcatagca 98580
ttagtgaaat ctcttttttt tttttttttt ttttttgaga cggagtctcg ttctgtcgcc 98640
caggcgggag tgcagtggcg cgatctccgc tcactgcaag ctccgccttc cgggttcacg 98700
ccattctcct gcctcagcct cccgagtagc tgggactaca ggcgcccgcc actgcgcccg 98760
gctaattttt tgtattttta gtagagacgg ggtttcaccg tggtctcgat ctcctgacct 98820
cgtgatccac ccgcctcggc ctcccaaagt gctgggatta caggcgtgag ccaccgcgcc 98880
cggccgtgaa atctcttttt aattgtttca agtaaagaca tttgtcattg tctctgtacc 98940
atattgtgct tcttctacag actcttaaaa ttcatgtatg tcaatcttat tgctcagaat 99000
cacaaaagta aagattcaag ctccttgata tctgtctaat atataaattc tttctttcac 99060
ttatccatgt tctggagact ttgagagtca tctagtccaa aaataagaag gcaaaattca 99120
gaatctctgg aaaatggtgc ccccaggttt ctaaatgact actgactgtg tccaaaatct 99180
gctacagtta aaacaagcca ttcatctctg aatctttatt ttaaaatccc atacctattg 99240
tataaaatgc ttggttatca aatttgttag ttatatgttt atttgcaaga gagttcttcc 99300
caacttcacc gtgtccaagt agaattgctt ttaaaagtgt tatctttctc agctctgctg 99360
atcacaagct atttgacgtc agatctcttg acttcagttt aggcatcatc attaccccct 99420
gacctcctgc agctctgagg aagacacagg acattctcta gcttggtcca tcttgatgag 99480
actactgaat gtaaaagggt tgggattggg acagagaggg caagtccatt tcaaagtttt 99540
gattgaggaa aaatcttttg gttggatcgt aattttacct ttcaaaaaca gacaatagta 99600
aaaagatagg actatggaag agaacataca cacacatgca cacagacaca cacacacaca 99660
cacacacaca cacacacaca cacacacaca cacacagagc tatggaggca gaattgccta 99720
gcaggaataa tggtagtttt gaaattaaat tatataaaga cctgggttta aatgctaagc 99780
tcaaaattat atcaagtgat tttaggtaag atagctaaca tttcttagct tcagtttccc 99840
cttggaaaac catagatatt aactatctat ggaactgatt cctggttcat agcagccatg 99900
catcaaatgg tacctactag agttctagga aagaaaacaa agagttgaga aagtgattat 99960
accttgaatt ttagtcaaag cagagtgaaa aggttagcta gagttttcct ctgtgtaata 100020
tactgagaac aggattcatg tgttagtaat ttattctaca ctaataggta ctgattattc 100080
agggaaaaaa acatgaaata taatacatat gtgtgtatat actgcatata tatatatata 100140
taaaaaatac ttattatgta atatgcgtgc acatggcgag cacgatactc ccctgccatc 100200
ttgtggtagc acagctatat tatattttca agtgtaatga attttattct aatgccatgg 100260
gttcatggca aataaataaa acaaattaat ttaaaaacac acacacacac ggagagaaag 100320
agagagaaga gtttttttta tcagcataca catttttcaa caactttgat gattttgttt 100380
tgcaaataaa tggatgcaat tttatttttg caggaagaaa ctcattaatt gattatttcc 100440
accttttatt gagtgctttc catgtatggg cctgtgtcag gtcctggagg cacatggctt 100500
aataagaata tattgtttgt cctcaaggta gaactgatga tgaatgctat tacaatgttg 100560
gagccatgac tgagagatgc tctagaaatc ctttggctga accacctagc tcagagtggt 100620
gcattgagag atacttacaa aaaaagaaaa cactatggtt gcgtattaaa agggtggtag 100680
aaattagtca aatagagtaa aactttgggg agtagagtgg tagaagaagt ggaatcaagg 100740
cagagggaat atgatgattg agataaggag taaaaatttc atttcaattc ctaatgtact 100800
caccactgat ttgccaggta tcctgctagg tgctgggatg ccagagtaga caaggcagat 100860
attgtccctg gctctcttca ggatcttttt ttcctcttcc ccttctcaaa atctatgaac 100920
taaaagggaa gggtttctat tcttgggcac acctgtttta ttctttgttt cttgactatc 100980
atcaggtttt ttacagcaag tatgctctgt taatgaatat ttttgactat gttgtaacat 101040
gaaagttgca tttcacaagg gaagtgatac aaatgtgagg aacttaatga aaagaagtag 101100
tagatcatgc taaaacaaag ggatttgccc tgatattgaa ggttggaaaa gtcaaacgtg 101160
accgccatga caaagtggca tttaatataa agaatgaagt aagaatgaga cttaaccagg 101220
tagggggaaa gattaatggc tgagagaaag aacttttgct gcactgggaa cagtgtgaag 101280
cccagcacca ggcaccatct tttcctctga aaaacagaag ggagttttat gtagatagac 101340
agtaaagtag agtagttatt aaatgaggcc agggccacta tccaagatat taacaatcta 101400
atcctggaaa ctgtgaatac actacatgac aaaagataat tgaggatgca gatggaataa 101460
gattgctaat cctctgacta caatggggaa atgaccctgg actgtcgagg tgggttcagt 101520
gaaaccactt gaggtcttag aagtaggaaa gggagggaga agaacgagtc agaggaatga 101580
attagaagag ggaggagaga ttccacaaat gacagagaac tggcccatga ctgttgactt 101640
taaagacaaa tgctgcggtc aagggccaaa gaatccaggt ggtctctgaa agctagaaaa 101700
gacaagaaag tgaattatcc tatggagctt atagaaaaga atgctgccct gtagacacct 101760
tgatgtttac cacagtgaga ctcctgttgg gcttctggct tcaagaacta taagataata 101820
catttttgct gttttagacc agtaaatttg tggtcatttg ttatgacagc aacagaaaat 101880
tactacatta gatgtattgt cagtggtaga ttagggaagc cctctaagct acgttaagga 101940
ttttattcct tatgatcaag cggtcactat tccatagctc aggaaggaaa atggcatgtt 102000
tgcgtatgca aagatcacac caactggttt cactgagact caatgggtgg gagcaatagt 102060
ggacccaggg gaacctggga aaggggctgt tgcagttgtt tgctaatatg tggtagtgat 102120
ggcagactgg ggaaaaaaac agacttattc atttcagaga tacttaggtg ggaaaactga 102180
tgatgtttcc tacttgatgg atggaaatgg taagacaaag ttgggactta ataccaaaaa 102240
tgtgagtttt attgtgcaaa ttaaaaaatg aattttaaga aaagaaaatc agagattggc 102300
tcgttagata tattgttcca ttacataatg atgtcagtga gccttggaat agcagtcaaa 102360
aatattcagt aacagaacat acattgcttc caagacctga tgatagtcaa gagacaaaga 102420
atagaacagg tgtgtcaaag aaaagaaacc cttcccttat ttttcagatt ttaaaagggg 102480
gggaagagaa agcacaagat cctgaagaca gccaaaaaat aaagaaaaga gaggctatgt 102540
cataaaagag ggaggagggg tgacttcatt gaataaatcg tttttaaatc tctatttctc 102600
agaaatgggg ctaaatagtg gggattaaat gttaccaaga gcaagacacc ttgcccttag 102660
attctatttg gaaggaagac aagcaagtaa ctgggtaatt ttcaaatggt ccagtagaca 102720
tatgttgtct tgcgcgatgc tcctactgat gttgaatacc tttccatatg ctattggcta 102780
gtcagaatcc ctctgacata tcctgctccc attttctatt attgtttatt accattatac 102840
taatttataa gagcttgttg tatattcata tactattcat ttgtctatta aatgttttgc 102900
aaatattttc ttcaatttta atttttccat gctaacattg tttacagtat tttttctttt 102960
ttttcttcat gtatccaaat ctgacttaaa ctttaaaaac tttataatca ggatagtttt 103020
actttatgtc tttgatattt tggttttttc ttaggaagtc cttacttgtc tttatttata 103080
tatatatttt agacagagtt ttgctcttat cgctcaggct ggagtacgat ggcacaatct 103140
tggctcacca caacctccgc ctcccaggtt caagtgattc tcctgcctcg gcctcctgag 103200
tacctgggat tacaggcatg caccgccaca ctccgctaat tttgtatttt tagtagaggc 103260
agggtttttt catgttggtc aggctggtct tgaactcccc acctcaggtg atccgccctc 103320
ctcagcttcc caaagtgctg ggattacatg cgtgagtcac cgtgcctggc caatatattc 103380
ttataaatat attcatctat agttttttat tgatatctgg tacttgtaca tatttatggg 103440
atacatgtga tattttgata gatgaataca atgtgtaatg atcaaattgg ggtcataatt 103500
ttagcatctc tattgatgtt taattaacat aaagtgcaca tatttattat gtacaatttg 103560
ataagtttta aaatagatat ctacctgtaa aaccatcatc acaatcaaga agataaacat 103620
atctatcacc acaaaagttt tctcaggctt ctttgtaatc tcttccacca ccctccacac 103680
ttgtctacag agaagtgtca atctttttct gtcactaaaa gttagtttgc atttctggaa 103740
acttatataa atagaagcat gcaatatgca cccttttatg tcttttacta agcacaacta 103800
aactactgag atttatctgt cttgttgcgt gtatcaatac ctcattcctt ttcattgttt 103860
agtagtattt catcatatag ctatagcaca aacttttttt acccagactt gttttgaaca 103920
gttgtgttgt ttccactttt tgccattgca aataaagctg ctgtaagcat ttgtgtatgg 103980
gcctgggtat aaacatgctt ttagttttct ctgttaacta tctagaagtg gagtggctgg 104040
accatatgaa aggaatatgt ttaactgttt aataaactgc caaattgttt tccaaagcaa 104100
ttgtagcatt ttacattctg accagcattt tatgagggtc ccggtttctc catgtccttt 104160
ccattattta ttatgatcag tcttttccat tgtagcaagt gtaatgcatg tgtagtggta 104220
tcccattgca gttttagttt gcacttctct aatgactaac agcgttaagt gtcttttccc 104280
ccctaggttt atctgccatt gtgtatctct tttttggtta agtatgtatt cagatctttt 104340
ccccattctt aattacgtta tttgtagaat ctgtttcaag actgatggcc attgtagtca 104400
gtgatcatgt ttctgaaaat caatcaggga agtcacctat ttttcaaagt cagatggtga 104460
gcaaaagcct tactccatga acacatttct gaaagctggc caatcaacaa taaacttacc 104520
tgagcagggt gtagtgcctc acacctgtag tctcagctac ttgggaggct gaggcaggag 104580
gatctcttga gcccaggaac ttgaggctgc agtgagctac gatcacatca ctgcactcca 104640
tctgggcaac atagcaagac cctgtttctg aaaaaacaac aaatagctta cctgaataag 104700
cacatttcca ctgctgagat ccaccaactc ctgactttga aagtcggccg gtccctggct 104760
ctacctcttc caaatcctca tacaaaataa gcttcctgct ttgcaatcag ggaaatggtg 104820
cctaaaaaac atagttctct cttatttaag taataaattc agattttttt ctaggtgttg 104880
agtggtatcc tctcccctca aaaccatgta gtttatcaaa aacttctttt tatgtaggtt 104940
ttgtggttta gaaaaagtct gttacttctt ttctgaaaga aatgaatttt tttatccatg 105000
tgtattgatg agttcatcct ttcttccctt atttaaatat ctcctttatc ataaactaaa 105060
ttactagata atcttaattt tatccaaaac acttagtcat aattttcttt gcatttccat 105120
ctttttttcc tacaaacgcc aagtgctagt taccaaatgg ttctactttt ctgacagcag 105180
aatctcttta cacatcttgt ataactcaaa aatcgagtta tgtgatatag ttagaggtag 105240
tcagtttatt tagtcttaca tctgaatatt tcaagtttat ctatttgatc ataaaacagg 105300
tttttttaaa tactcttggg ctttctcatg aaaagtagtg acagaacaaa tgcattgaaa 105360
agaagaaaga taatggtata gacaggtgtt ttctttcatg ttcaatcaaa aggtttttat 105420
tttcaactca gaatctgtga agcttcatga gatcagggct gttgtatttt tctttctaca 105480
tgcctggcaa ctggagcaat gccttccacg tggtgtatat taactattta tgggatgaat 105540
gatgaaatta atgaatgagt gaaataaata attttgtctt tctgtgtata tttatgctaa 105600
gcatataaca gggtaaaatt aagaggcgtt attcttattt acaagtaatt acaaaagggt 105660
gggttggaca acttagtttt tgtgagttaa atataaaagt aacaatgtat tctcactagg 105720
tgagaaatca taatttgcca gacaaatggt gtagactctg aatacttgga aaggtataag 105780
tcactgggga ctaaagagat tgagtctccc taaatacagg cctacatttg gacactgaaa 105840
tgatgttgat atatatattt taacatgtca ttctgtcttt taatctcctt atatgtaata 105900
cttgtttcct tcttcctccc attcaccttt ttctttaagt agcagtttgg tcacttagcg 105960
cttgactcat tcaagccgtt gaatgacagc accatgttag agatgcccaa aggtcaacca 106020
gaaagaaaca aagacgattt tgttcctaga tttatagttt taaatgaact cagcatagtt 106080
ttagtctata ttctcaaaca ttgtcatttc cccaatattc ttaaaatctt tcaaaccttg 106140
aaagtcggca cataagaata taatgattgt ctcataccta cacatagcgt attcactttc 106200
ttgttgatca tgttattgtt ctattactat caaaataatc attgtggaaa ggtatgaaaa 106260
tctgacacac tgaaacaaat gaaccagaag agattaagaa gacatcatag aaagtgcaat 106320
gtgctaactt ggactggatc ctggaacaga aaagtggaaa actggtggaa aaactggtga 106380
aatacaaatg aggtctgaaa gtagtcaata gaaatattta atagtaatgt aaccaaagtc 106440
aagttcttag ctttgacaaa tgttccacag tcatgtaagc tattagcata agtggaagct 106500
ggattatact tatacgtgaa ctctgtttta tttctgcaac tttcctgtaa atataaaatt 106560
attctaaaaa taaaacgctt attaaaaact aaatataaat atatttttca gtaagtatac 106620
tgaagtgatt tctaaattaa ttatattatt attacatcaa aaatgagact tttactcatc 106680
ttgttttgtt tactgcgggt agcacatgga aaatgctcaa gtcactgaat tatagagcac 106740
caatgaataa ttttctttag ttttcaatat gagaagagtg tattctccac ttcagccacc 106800
aggtggaagt atcagcacat cagttaaaat cagttcttca ttgggaggtt taaaaactac 106860
ccttggaaaa caaacagttt ttaatgttat gatatgatgt tattatacac agtaagagga 106920
agaattccca gtattatgat gaaaatcaag aaagatataa attggctgtg gacaggactc 106980
caggatactg agatatccag aaataaagtg aaaaaaaatt ttaccattta tttaaacatt 107040
tccagacatg gatataagtg cacatggata aaagttgaac aggtagcaac atctggagac 107100
ttaacggaca tttatcttag cccagacata agtgtagcat gcaatgtctg aaactaccaa 107160
ggcatggaaa gctaaactga tcagtatgat gctgtcagtg ctttcagtat gggtgatatt 107220
caaagtacag gagaaccatt tagactaaat gctaccattt aatggcccaa tctgcattct 107280
ccctgctacc atcttgatga cactttctac cattcaggaa atgaaagcac tttctagtgt 107340
cacccagtgc aatatctaat taggaaggca aattataaaa ataaaaatgg aactgcagtg 107400
tctttctatc caaaaacgag gcttgctttg tagtttctaa tccatcagtt tctttcctta 107460
ctttgaataa tcaggcattt gttatgtcta attttatgtc aatcacattc tcaactctct 107520
gtataaactc tttcaataaa gattgataca ggtatgagaa gaaccagaat tttttcaaaa 107580
atattatatt agacacaatt taatataagg tgaaatctat tgattcagtc acagtttgat 107640
ctgttatttt cacataccac attctgtaag atgctcacag gctactgaaa taaataatga 107700
tttgcctttt tccattttct gagttataca agtttttaga gatgtgtctt gtgtaaagtg 107760
catcattgcc tgcttttcaa ttaaaaagac ttttgaggcc tgagaaatta gaccaaaaaa 107820
ggattaatca taataatctt ttaaatagag aacacctgcc atcaatattt tagaaagtat 107880
cattctaaaa ctaaaacttc tattactaag agattataat aacaggcaac cctacttatt 107940
tctaagaata gggtctacat gtttttactt attggtccac atattctctg caattaaaac 108000
ttagacaaca tttgagtaat gttgtccatg ttgactgatg accacagaca cagtgatgat 108060
ataaatctta aacagattca tttgatttcc tcatcagtga tgaactacaa cattctcctt 108120
catgatttct tttcttatgt ccatacctat ccatttaatc ttaattctgg ttatttggaa 108180
caaaaattcc aataagtggc atttttccag ggctcacatc taatagatca agacctctag 108240
tgctttgatg cttaggtgtg gaaagagctc tttaccagga atgccgggca ctctgaatta 108300
gtcctgtctg ctcagaagaa cattgcctct ccttaccaat tttctaatta taacgaacat 108360
acacaattgt taaaaattct tgccagcctt tggacaatta caaaacagca tttttcatca 108420
aaggacacct ggagtatgac ttataggcac ttacaatcca gaaagataag gtaatcaaac 108480
tctggaagaa ttctgccagc cgggaccctt taggtagaag gcagtgaggt agacttccgt 108540
ggatctacca gggcaacaaa agagaagagg cagagccctt ctgagctatt cagcagggag 108600
aagactgaga tgctgaaaat gtggctaaag acacagtagt actgtgagtc ctcaatataa 108660
cccatatttt tttccaactt gtgttaatct aatacatctt ttgaatataa aggaagaaag 108720
tgtaaaatgc agttggtgat ttggttgttc tatttttgct ctcttttcct ttatacaaca 108780
gaaaattttg gttattcaat attgtactta aaggcataag gcatgtgtgt atgtatatat 108840
acacacataa atatgtatta tatatttaca tattacatgt attataatat atacatatta 108900
tatattatat atacatatta tattttaata tgtaaaatat tctgtcattt gtatttacaa 108960
atatgtcaag tttgttttag gataggcaaa atactctaat aagtaaaaat ttcacctttt 109020
actagtagtt ttgatgctat atccttggaa tttcagggtt ttcatagaaa atgtttttta 109080
aagaaaagaa aaaaatggta gaaatttcat tgtcttattt tttaaggacc attgcttaat 109140
tacttttttc tcagttctgt ttatttgctc ttaaaacaaa agaaacaaca aaaactgatg 109200
gctttctcgg acaacaacaa catccacaac aacaaaaaaa acactaatca gtttctgaaa 109260
aactaaagag taactgatta ttctaactat taactacccg accattcagg tcattgttct 109320
tttctacagt gtgtgtttat ctcatgctca tatattggtt ttttaagtgc agtatactat 109380
acttaatcat tgtctaagat tctttggaaa tctgtttttc tattcaattt atactcctag 109440
tagaaaattt tttggggaac aaaaaactga accatcataa ggctaaatga aactccttgg 109500
aagtaaattg ttatttgagg tatctaaatc agaaatttct gttcttcagt tttatgtgtc 109560
tggggtattg ctgtaaagca ctgaccattt ctttgctgga acatagttta tacataatgg 109620
agaaggaata ggccaaggag agatgatgat gaatgtatgc tttcccctgc agaaatccct 109680
ttcaaaaaga gtaatactaa caaaaacaaa ggcaagttga aaatgtggtg gacctcaccc 109740
tcagaccagg tgagccttca gattctgcac ctcgctggcc gtgcataaag aaggctggga 109800
catccctgaa ttcacagtca gggtggtcgt ctgtgtgcag aactgaaagc aaacaagagt 109860
aagctatagc cagatgtggg ggctttcagg ttcactgacg gttcctgggg tacttggagt 109920
caaagcagct cacgcacgtt cctgctttcc accaggaaac gtggagccac ggaggccaag 109980
gaaatctgat cagctccaga tccccaagtt ggtcttttta cacccctaaa gacaccatgc 110040
ttgctccttc tccaagactc ataaggaaat acaaatgact ttgaaatatc agccactgtt 110100
aactggatca gattcctcca gtaaatatgg taagccaaca gatctacctt atggctcaag 110160
atccagcaaa gcatctcata aaagaatatt tctatgccct catccataca tgtgtttcta 110220
gagatatgtt atcagtcctt tctaccaagt agacagaatt tttatcatta catctttgaa 110280
atcatagaaa atccttcttc tcagcggcat ctagttttac ttaatcctgc tcttcaccaa 110340
attctcagtt ccaaaatttt gattttttta ctgacttctg tcatttcctt cttttgattt 110400
gactttcttt gtcctgaacc tcaaatccca cctgatacac aaaaactatg aatttaaaag 110460
agcatattca ttgccatagt tttgtgaagt taaatcaact ttgcatatga attggcatag 110520
gaagatcaca ttcccttcca tacgaattta gagaaggtat gaaagtcagc tctgttagtt 110580
tcagagtgtt tggttcaacc tttgtgtgct cagtgttcgg gggtaaaggg tcatctgtct 110640
cttctatgtt tttttctaag ttggccgagt aatttcatct ttaggtctcc atttctaaaa 110700
ttacatcctg ggtttgataa gattaaaaga tgaagtgatt aattatggat ttatttaaca 110760
attgactgat ttttacaaat agttcacttt taagtggctt tgaacttatc tttgagcacc 110820
aaaaacttta ttggtgttct atatgtactg ggcaaatgaa accaaacttc attttacccc 110880
tattgaaagt ctgtttactg aagtcactaa atcaccccat ttctgacctc agctaggggt 110940
tcaaactctt tttcacagcc attaactttt tcagcgtttc accaagcaga tgtgtgttgt 111000
gtggcttaat cttttccata ctagatgata cagcaggcct ccacctgacg tgcgctttga 111060
tgcagcataa aaatctcatt catcatatct gatttgtcct ctttgttgac atatgattaa 111120
tgtaaaatgg tttttgtttt ttttttcaga gagaagagtc atgacttttg ctcagtcatt 111180
aaaataagag acacaaggat tggtatgggg tatccctagc agaatttatt caaggaagcc 111240
taatcaatat cccccatctc actatttctt cagacacgtg ctaagtttac atattcgaat 111300
caaattaaca tatcagagtc acaaattggc aatggtgtca aaatacctat cctcaccatt 111360
ctaaatcatt tttcacatta aatatgtcaa gggctatgtt tgcgtgcata agtcaaaaga 111420
agagaaataa agcaatacat taagaagttg gtgacataag cttatttatt tagttattta 111480
tttttgccta ttactggtag agaaaaatgt atttagtttg ctctattaca ggtagagaaa 111540
aattatattt tgttgttctg agtgaaacca gtgataatca ttttacctct tcttttaagt 111600
agtatagaag tctaacattt tcattgctta ttgccagaac ttaagtgcta agattttttc 111660
tatattgtct gatttgattc atacatataa tgttgagatt caagaattat tatcccattt 111720
accagatgag gaagtggagg tacagaagtt ttagaaaagg gaattagcct ccgcttctat 111780
tctattaagc cactatattt cagaaggcat agagtatcta ttttgcattt gggttggggg 111840
agatggagat ttgccctaac tttgattctt ttaggtcaag aataatttct ttcagaaccc 111900
ggatggtttt cttatttcca taaaatgggt tgccacatta ggcctcagag tccaccaaat 111960
gaacggttct tctcttctgc tgtccctcaa atttgtgata tgtaaaatat gtgtgaagat 112020
gcagataaca tgtgggatct cattggctct accattcctt agagaaaatg taattgttat 112080
tgattctaac tgatcattca aaaattaagt tcctccaaat ttactctttt tctaattgaa 112140
ggttccagct gccatgggat tgagatttcc tatttttttt tttcttgtcc cgaaactctc 112200
tgtcaaagat tttatttctc taggcaacca gctggttttt cacatagata aggtcaatta 112260
atttgacctt tggttcaata tgaaatgcat aataaaagct ggtgccccaa ggaccggatg 112320
tggcctttca aaaaagtaac ttgccaggac agggaacaat aatttattac ctcataaacc 112380
ccagttgaaa tgcctgttgc ccagcatagc aatgacaagg agcttgtgtg tctttattta 112440
tttattttta ttctatgcaa tagagagagg ggaggaaaat aaaaaaataa ataaataaaa 112500
caagcacctg ccccattcag ggaggaaaaa tggcaaaggc aaaaaagatc tactcgtact 112560
gcttgaattt ttgttttata gcaagttttc atccactaca caaaaattct tagagacagg 112620
ctcgggaaag tttattatca actataaaat tttgttaaat tgtatttcat cgcttgaatt 112680
ttgagagata caggaaatct ttgtgaagcg atttgaaata gagaaccaaa attgctatgt 112740
atagtcaaca tccaaaatga ctactaatca ggataaattt atctatggat ctgtttggtg 112800
gatatattta tatatctaat tttaacaaat tcatgaggct gtgaactctt atatttcagt 112860
ttttacttaa ttctgtatcg ctatcattct gtatgccatt agcatccttc atatgtggta 112920
tttattcctt tgcataaatg tgttataact ggcgtttgtg tctagtttta tctgatattt 112980
taaacaaccc tgaatgaaac tcctttacat aagtcattga tcacatcctt gattatgtct 113040
tagaaatggg cgatctcaac aaaacataca aatatcttaa agctattgaa aactattctc 113100
tcagtataat tcaccatttc tagccaccat gtagttttta atccattcct tcagctgttc 113160
ctttgaaata gtacttttag tatacgtgga attatggata aaacagactg tagaaaatat 113220
ttaaacctca aaatgtagac acgtgcctaa aacgaatctt agctctgtga atgcccttca 113280
ttggggaatt tttacagata atgagatgca agttttggaa ctccagtggg agttttgaat 113340
aaggggcaat ttctgagtaa tggtgtaatg gagtcatagt aatggtgcaa tagagtaatg 113400
gtgtaatacc ccacagtgtg gggtacaaga gaagctttga gaatctagaa tgcaccaggt 113460
ccttttaggt aggagacaaa aaatgcagcc aaagtgggga aggggtggaa aggaaaataa 113520
aatagtcctg gaaacaaaca aacctattac aaaattttta cacagctact tgtgtgacct 113580
tctggtgaga atgcaactta gaatcacttt cctagagagc tacttgggaa tatctatcta 113640
aaggcttaaa taacagatac tctgtctaag caaatacaat ttgaagtctt catacccaga 113700
aaatcatcat cgatatccca aacaactatt gtaacagaat ttaactttta caaataaaaa 113760
attaaataaa atttgtttca tctttccaaa attttttata tgcaaatagt gagaaagaga 113820
gagggagggg caaacaattt ataccaggta ttttgtttgc aactggcttc tttttattca 113880
tttaccaata tattaatggt ggttattgct ggtttctaat tttatggtga tactaaattt 113940
tcttattttt tcaaacttca aaacacaaag acaaaaagcc atcaacaaat gctattttgc 114000
tatatctata gatgattcag tactgcatat caaaatggga aaaatttctt ttcccatatt 114060
ctttcattca gtggttcccc atactcactt gtaaccgcaa actggatgta cggttaatgc 114120
catttgtgct ctatttcaaa gacattatta gttaaatagc cttcttttta agtatcttaa 114180
gaacctagcc acaatttata ttattgtttt catgggaaaa acatgttcca agtttcaaac 114240
agctagtgta aaaacaaatc caaaacagtt tgcatgtatg ttgaagaccg tctattcagt 114300
tttttgtttg tttgttttta ctcctgaaag ctagttggtg tcttgaagtc atgaattgcc 114360
tgagagtaca atatacccag tgccggaaat tgtggcctga cctttagtta agaaagactt 114420
ccttctagat ctctgtctta cagagaccat tctttccatg agaatttctt tattattttt 114480
gaagttatca attgaaatca caatttactg atttgtgtgt gaagtaagtc tgacacaaaa 114540
cagggaatca tatttaagac tactaagtta tccccttctc attacttttt ttttgtttga 114600
ttgtagaaaa acctctatag agccatatct ctctaatact tgtattatat ttatctccag 114660
agatctagct tgctcaaaag gagactatta tctgactgcc tgaatagatc ccattacagg 114720
aaaaattgac tgaaatattg tcaaatgtgt tccagcaata aaattcaggt aataagttga 114780
atttctagct ttctgtgtcc agatttcccc tatatgattc taaacaatag ttggggcagg 114840
tcaagaaaaa gaggttaaga gacatgatgg taaagaaaga ccaggaagag gatgaaaact 114900
ttgtgagtct caggaacata aaactgccag ttgtttgcag caaaattctc acgaccccaa 114960
agctgaactt tgtaaacagg cttggcatca aactcttgcc tggagaaaat aaggaagaaa 115020
agaaagaaaa aaaagagaag acccttgccc tgcatacaga cacacacaca cacacacaca 115080
cacacacaca cgcacacata tggcatgtgt gcactgctta aatttgtaag gacatataca 115140
actggcccgt ttctaaataa gtcttttaat atcctgagca gtttgtatta ggctgagtga 115200
tagaattatt tgtattggcc aattatttcc aactctgttt cctggagagg ttatttctaa 115260
tttttttagt attataaagt tcctaatatg tgaggaggag gtatgatata tgatgaaaac 115320
catgaattta agtgttgatt gggtctgagt ttcaactcaa ctaacttact cgatttaaag 115380
ttatttgatt ttttttttgt tttgcccttt aaaactgtga aaatcatagc atgtatctct 115440
tttaattatt gtgagaattg aaagacatgc acactaactg atcacactta tttagaaagc 115500
ttagattaat gcctgaccac agtgagtcta caataaatat tggatataat tattcttgcc 115560
attgttatta gaagaaattg ttaattgaat cagagacctg gaatgaaata atccccagat 115620
ataggcatct taaccttagc aatctctttt tgagtacaaa aagggagctt attttaatgt 115680
ataacaggaa acatccacac ttccctcata tgcctccata gttaggaatt tgaacctatt 115740
tctttattac aataatggaa attttcatca aatgggctag aactaatttt caaacatggt 115800
tgctatgaca tctttcatag tttttatcac attataagta aatgtgtttt actcatggtc 115860
atgcctagca ataaagaaat aatagcactc aatgcaaatg gatggcttaa aaattggaca 115920
ttagatgtga accagggcct gacttttcat ataaaaggtt ttatgtattt taaatgtgac 115980
tcagtaattt ctatttgaat gctgaatgtg tctgtctcta tttttcatag acatgatggg 116040
taaattccac agtaataaat atcttgaaaa tttctgtcta ataaaattaa tgttatccag 116100
taacccactt gattataata tattttctac aattgatatt atatagtcct ttgacatttg 116160
ttgatattgg ctgtgatatt tattaaggtt atctttaata atttcattta tgagattcaa 116220
accttcatca ctttattttc tcagatgacc tgataaatct cttatgtcta tcattttcta 116280
ataatatttt ctatcctaca aaataacttt cttccagtct tacgcttttc gatacctggt 116340
gtatttgtaa gtgttgctta atattttccc ccctctctca tctcttgaga cttactttaa 116400
gtttttcttt tcagttactt tatttttttc aactctctaa atgccattat taatattttc 116460
tgggttttag gttcattata tgttagattt caaaattaga ccacaagatt tatgtgccta 116520
ttatttcaca tatattgtag tttagtcatc atgaagttat cccttgatac cccagctcaa 116580
ctttgacctt gtgacaaaat attgttctgc ctttctattc acatgggtat gtgaatttct 116640
caccaagatt ctgcatcata gacttttgtg ttagatttcc agatacacca acaagcctaa 116700
ttgaaaaaat gggaatgtgc atatttaccc tccctttgtt tctctcatta tatgtttttg 116760
cagtttagta gataactatc ctcatacttc tagcttgtta gatctagtag ttgtggacct 116820
gtgaggtaaa ctcacaaaag atagattagc aaggacaaaa acagattgaa ttacatatgt 116880
acgtacaggt attcacatag aaatgtgtca aaaggaaatg tctagaattt gggcctcata 116940
taccatctta acaaaggatg ataaattgtg gagaagtaac tatacaaagg aagggagggc 117000
actgaacttc cagggcatgt aaattgtgga aatgtaagta tgaggaaaac taatggaata 117060
taagggttat ttagttaaga agtgttatgt agactcaaga gctttctctg ttgataagag 117120
gtatctccac tgacagagtt gtcctcctct ttctgataca aaaaagaaag atacctttac 117180
aaatggaaat ttgtgttacg tttacaaagg aaaatttatg ccccgctttt agacagaaag 117240
gtggagggca gagagctttt tctgtatttg ctattcctaa attgcaatca gcttaaaata 117300
agccttatct caaagcagaa tattttgggc taatatacac caatccccat ctgtgtctgt 117360
tctttctttc tttctttctt ttttctttct tttttttttt tttttttttt tttttgctgt 117420
ctctaaacaa ttgagttttg aagtttggct ggtactttcc acatattatt ttttgttttt 117480
gttgttaatc ttgtacttat tttgtctcac tggctcctga ttttaaataa tctgtatcgc 117540
ttttcacaaa tataaacagt gtaacatgtt tataaaacat tttcaaacat ttaacgtttg 117600
taaaatttaa aaaccacccc caattctgac atccagtgat ggtccttatt aacattttga 117660
tgtgttttat ttttgttgca gtatgtattt ctgtaagtat gtgtgtatta acaaagctca 117720
ctaatacttt ataatcaaat tctctgctgg tttcatattc tccattcatc cacttgcacc 117780
ttaaatatca gcaaactgac ttgttcctgg aacacgctgg ttcaccttta catatgcacc 117840
actgtatata gcattccttt atattatttc ttcagcttag agcatcctaa tcaaccccat 117900
ccctaagctg aattttcatt ttacagtacc aaggcaccct tatatttgcc tttatatcaa 117960
aacacatttt atatgcatta taattttctt tcttgtccat gtcttcagct acacacagag 118020
cttaacaatt tatttttgtt gttgaaatct gctgaataac aagtttttaa taagtatttg 118080
ttaatcaaac atatgtttca atcattttct acctgtatca aagtaaatgc atcaatcaac 118140
acatgtttac taacaccttg ggtatacttc tatgctaggt gttgtatatg ctacaaagaa 118200
attaatcaca atgctgaata cagacaaatg ggagaagaaa gcaggtttgg aatcagaaga 118260
ctttgctatt tgttatgtgt tgatgaatac atttttgtat tctgcatgtt tcagatgaga 118320
aaactaagat taatgcccat ttcacaatat catttagaga attttcatag ttagtggttt 118380
cacaatgtga tgtcagtgtc aaatatactt gtattaaata tattttaaca ttaaatattt 118440
tttatttaaa attaaaataa tattaaaatg ttagtcttaa cattttaaat atttaattta 118500
acattaaaat attattgcta ctattactta attcatcaaa gtggccacaa tttaatgagt 118560
gtagcataaa gcatttgcag ttcttactgt cttgctgaaa atgcaaaatg tcaatttcag 118620
tctttgtctt aaactcataa ttaaaactta gaggcagttc aaccctaaat cattacaagg 118680
tgtgtgctca cactttctgg aaacttggat agtgcttctt attgcattgg atacctgaca 118740
aaacaaagat gttgcctaca aaaaacaaca aagttaatga gactttttaa aaatcgacta 118800
taaacaaagg aaccagggct ctccaaaata aaaaagtaca ttattttatt ttaaagtctt 118860
aaagtttaga tagattactg aagcccaagg aactgccact ccatagcttt tccttcagac 118920
atattactag ggtctcccat attcttaaag tgttattcta cagttggcaa gatctttagg 118980
ttgacccttc tgtgaactag gaggtctcga gtgaaatttg tatgaatcct atgagttcta 119040
aattctattt ggcttacaca acgtgtaact cagcattctc acagatttta gtttgctttt 119100
acttttagca tttaatcctc aaaagtttgt tatatagata atgtttcagt gaacagctat 119160
ttagcactat tattactatc ttctgcttaa gattgttttc aataatgcaa aaccaaaatt 119220
caaaacccat acaaaaagag atcatcagaa tgtgttcata caattctgtt atgtgagtta 119280
gcattttttt tgcttattta tgcaacctca aacttttccc tcatcctgta aaattcagca 119340
ttctcttgta attcagaaat tctcagtgat aaagttctgc tttaattagt attgaggatt 119400
atttttttaa aaagggcgag agagaaagag aaggagagct gagagaataa gcacaggaaa 119460
ttactagaat aacaacatga caactggaga ggcatcacac ttcaaataga caaatagggc 119520
acaataatgg atcccaaatg tttttatttt ttttcttaat ttctgtgttt ccataagaaa 119580
gctagagaat agtttaagct gaagccttat cactagagcc gaaaaataat aaacacaaaa 119640
aactctttaa gaagtaataa aatataagaa aacaatattt taaaatttaa gtatgttaat 119700
aaagcatttc tctttttttt ttttggctgt tacctactta ttgtttgtga tgattttact 119760
cagtaccctg tggtttcctc cctggttgct ttgcctgtct ttcagatact tttctaagga 119820
cttccgttat ttgataagta gtatcaaaat tcttttttga taggttaggg tctaattata 119880
tatgtagata gccagaatca aagcagaata aatgtttatt tgtttgtttt atgttttgtt 119940
tttttgagac agtgtctcac tgtgtcaccc aggctggagt gcactggtgc aatcttggct 120000
cactgcaacc tccgcctccc gggctcaagc catcctacca cctcagcctc ccaagtacct 120060
gggaccacag gcgcatacca ccatggccgg ctaatttgtt ttgtattttt ttttggagat 120120
agggtttcac catgttgccc agattggtct tgaactcttg gactcaagcg atccgtccac 120180
ctgggtctcc caaagtcctg ggattacagg cataagccac ctcacctgac taaagaagaa 120240
taaattttta aaatgtttac tttcatttgt tgtttcaaat ataagaaaat agtcttatga 120300
ttatacaatg tagtattaat aacaatttat cattgtatta tttcttatac attgtaattt 120360
attgcaatca caactttatt aatataatta tttatattta cgatactcat ataatagtat 120420
attgatataa tagtcaacat ttcttgagtg tttaccatgt accaattatt ataagtgaat 120480
tatcaaaaat tatttatttt aatatttaat atcattatga gacaattact attatcccaa 120540
ttatagaaaa aaatgaggat cagagaggat agataacttg tccaaaatct tgtagtagtg 120600
agattataag tctagctatc cctgtaaaaa ctatgctatg tcatatctaa aagcctatgt 120660
agcagatagg acaggtatat gataatcatt ttgcaaatac agacacaaaa atttatgttc 120720
tcattactca acttgctcat aaacactcag caagctatct gaaaagacta aaagtgtgag 120780
gttttttttt ttttttttgc ttttttaaac atttattttt gattcagggg gacctgggaa 120840
gtttttttat ataggtaaac tcatgtcata ggggtttgtt gcatagatta tttcatgccc 120900
caggtattaa gcctagtaca caacagttat tttttctgct cttctccatc ctcctaccct 120960
tcaccgtcaa gtagatccca gtgtgtgttg tttccttctt tgtgttcatg agttcccatc 121020
atttagtttc cacttataag tgaaaacatg cagtatttga ttttctattc ctgtgttagt 121080
ttgctaagga taatagccac gaactccatc aatgttcctg caaaagacat gatctcattc 121140
tttatggctg catagaattt catggtgtat atgtaccaca ttttatttat acaatctgcg 121200
gttgatgggc atttgagttg attccatgtt tttgctattg tgaaaaatgc tgcagtgaac 121260
atacacatgc gtgtgtcttt atggtataat gatttatatt cctctgggtt tatacccagt 121320
aatgggattg ctgggttgaa tgatagttct gcttttagca gtttgaggaa tcaccatact 121380
gttttccaca atggttgaat taatttgcac tcccactaac agtgtataag cattctcttt 121440
tctccacaac ctcaccaaca tgtgttattt tttgactttt taataaaagt tattctgact 121500
ggcgtgagat ggtatctcat tgtggttttg atttgtattt ctctaatgat cagtgatact 121560
gagcttttct tcatatgctt ggtgactgca tgtatgtctt tttttgaaaa agtctgtttt 121620
ttaataacta gtgagatttc tcacttcatc acaatatttt ttaatttaga ttttctcatt 121680
taaatttctt accacttttt agacatagat gcattcttta tatttcatct gttttcagat 121740
gaatattaat tgacaatttg gtactagatc ctcctttaat tttttgttca tgacttgtct 121800
gtccacagta aaaaaaaaaa gtataaatag tagcaaaata tagaaaccaa tggttttcag 121860
gcattggata acaggaaatt caagtctaca attttttttt tttttttgag acagagtctt 121920
gctctgtcgc tcaggctgga gttcagtggc gcaaccttgg ctcacttcat ctctgcctcc 121980
tgggttcaag caattctcct gcctcagcct cccaagtagc ttggattaca agtgtatgcc 122040
accatgcccg gctaattttt gtatttttag tagagatggg gtttcgccat gttggccagg 122100
ctggtctcga tgtcctgacc tcaggtgatc tgcccacctc ggcctccgaa agtgctggga 122160
ttacaggtgt gtgccaccaa acccggcctc caattcttaa gagaaggtaa acatattccc 122220
tgtctctctg cctggggata tgttcatacc tcaaggcaga tcaaattaga gctcaagtag 122280
tgaagcaata cctctgagct gataaggcaa atatgagttc caggccacta aaacaactga 122340
aatttgctga gcaggatcac agaaaatagg ggctacataa agtaaaggac ccagaagtct 122400
gcatgagaat ttaccatggg atcttgactg agagaagttg tacaggcata ggataaaaaa 122460
ctgtgagaca gcagaaattt tttcttacag agaagatggc agaatcatga taccaaaggc 122520
cttttaagca tggatgatag aattttggtc caggaagagt agagaaacct ctctaataaa 122580
ctaatatatt caagtgagat ccaagaaaat taataactat taataaagac tacattctaa 122640
aataaagccc acatcctaag tatcaactta aaattgaaat cattatgtca taaaaaataa 122700
taaaatcaag cttcacaatg acagtaggat ctgccattat tgaacatatt gtcaagaaag 122760
aaagttggca caatccagat gtcttataac acatcactgt aatgtccaga atatgataca 122820
aattactagg catgtgaaaa aaaaaaaacc agcgaaaatg tgactttaaa aaaaaagtca 122880
atacaaatag atcatgcaat atcccagata ttgtaattaa ctgataaagc catttaaagt 122940
tcaaggacct aaaggaaaaa aaaaagtgac aatgagtgaa cagatgagaa aactcaatag 123000
ataaattaaa actaaaaaaa aactgaaatt atagaactga ggagtccaaa atctaaaatt 123060
aaaaagaaaa aactgggttt aagggcagtt tagacgctga ttacaaaaaa agctttgtca 123120
acttaaaaac agatcaacag aagatatata ttttaaaata cagagagaaa aagattgaca 123180
gaaaattagc agaacttatt ccttttgtct aactgaaact ggtgaatata gttaataaca 123240
gtataacata tttaaaaatt gctgagagta gatttttaaa ggttctcact acaaaagaat 123300
gagtaggttt ggtgatgcat atgttaatta gcttgattta attattccac tgtgtgtgtg 123360
tatcaaaaga tcacactgta ccacataaat atatacaatt attatttgtt acaattattt 123420
aaataaaata tacataactt taaaaagcaa ttaaaaagaa aaataaataa aattaacaga 123480
gcctcagtga cctatgcaaa aatatgaagt ggtctaacac acaggcagtg gtgtattagg 123540
aaggagaaaa agaaggggca gaaaatattt ttttgaagaa atttcctcct cttgtaaaag 123600
caaaaaaaaa atcacctttc atatccaagg agctcaataa atgccaagaa gaataaatac 123660
aaaaaaaact catatctaga catatcaaaa ttatactgct atcataaaaa gctaagataa 123720
agaaacattt tataatgtgc caaaggcaaa aacaaaacaa aacaaaacag atcacaattc 123780
ggggaacgat tttataaaaa cagttgactt cttatccgta gtaatgggtg ctagaaaaca 123840
atcgaacaac aaatatgaag taacagaaga aaaacagaaa ttgacctgaa attctgtact 123900
cagtgaaaat atccttcaca aatgatggag atgcgaagac aatgccagtc aaacaaaagc 123960
tgagagaatt aatggtcatc aacattttaa ggatgctgga aaaagttctt caggctgaag 124020
gaaagtatta attgatggaa gtagtttcca ggaaaataaa ctgtcctcaa aaacatagaa 124080
tctagcacag taattttcat caaacaatgg cctgtggggc tcagtccagc ccactgactg 124140
tattgtaaag ttttattgga acacagccac gtctgtacat ttatatatta tctatgacta 124200
ctttggtcct acgattacaa attgagtttc aacagagatc atatttcacc tttacagaag 124260
gaaaaattgc taagtcttat tgtaggggat aaaaaagcag acaacataat aaaacataac 124320
aagactaata tataatcacg tatagcacaa atgtgtgcat tgagaaaatt caaatatctc 124380
tttaaacaaa aaaatagagc tacttggaaa ttttcactgg ttggatgaga gatcagaaaa 124440
gtcaaaatgt atgaatgttg agtaaatagc cactgcctag ggcatttcat ccaccagaac 124500
agaagaatgg caaatgattc tttgatactt ccagtgttag attcaagaag tggcatttaa 124560
aaagtatgat tctcttgatt gtatcagatt ttcaaattta ctgcaaggaa cactaaattt 124620
tgaattagga tccccacttt tatgcaaaat aagtaaatac ataaagcatt tatggatggg 124680
tttatcctga gctcagcttt tataaggctt acttccgtaa ggtataaatt tataaaaaat 124740
cctttcacag attggagatt tgtcgccaga tttgtctggc tattagattg taacagcgca 124800
ggtgggaatt tagggaaata aacagtactc ataaggacct gttgattaat ttgagctcag 124860
cctttctatc cctaatattc tctttccttt ctctctctgc acatctgtga cactggagtt 124920
tggcccagag gaagaacgca cagtggagat aaagagatgt gtgccaagaa agccgaatat 124980
tcagatcaga tgggatgcag atgaacatct gtgctgggaa aaaaaaaaaa aaaagaaaaa 125040
aaaaagccaa ggacagatac tctcccaatg ttgaaataac tgtacagaat tctaccccta 125100
caaaatctct ctttttttat agagtttttc atttaaagaa atagtgtttc tgcactgcac 125160
ttaaaggtta tttgcctgct cctttgcagg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg 125220
tgtttctttt ttcagtttac caattcaaag gcgcaggcca acctcctatt atttgtgtcc 125280
atatttaaag tgggcttgac agtcagattt ttgcactgac aggcacacaa taaaaatcat 125340
gtactgttac tagttttcta ggcagaatta tttcataaca attatcttca catctccaat 125400
gttctgtgtg ctcaaaacca g 125421
<210>4
<211>599
<212>DNA
<213>Homo sapiens
<400>4
ccctgacctc aggtgagccg cccgcctttg tctcccaaag ttctaggatt acacgcgtgt 60
gccactactc agcctagctt cgttcattct atgctataat gtaaaagaat ctggacattg 120
catatgaata tatacaggag gacactcctg aagaagttat ctttttcctt cctggcagag 180
tttttaacct taaaaagcca gtttcttaat ggctttttcc acacagtctt caaagaaaat 240
tgctgtggtc attagcagtg ggtggtgtat ggagatttaa ttgaggactt agaagcaggy 300
caagtgaatg ctcgctagtg tggtagaggc tgcttagaga acactgaaga tggcgttgga 360
tgtgtgagaa cagagaggaa aaccaagaaa agtaacaaag atggtaaaat gtacgcttat 420
tttattgcta tcatctgcct taagtggaaa ttttatttat ttattaattt ttttactttt 480
agaggtagag tctcatactg ttgccaaggc cgcagtacag tagcatgatc atggctcact 540
gcaacttaaa ttcctggact caagtgattc ccccaaccac agcctcctcc tgagtagct 599
<210>5
<211>599
<212>DNA
<213>Homo sapiens
<400>5
tacaaggtgg ataatactga ataatggcta agattcagaa taatatatac tagaaactgc 60
atgcataaat tccctggtca ccattaacat tgtgtttttc ttaatgcctg aaataacatc 120
taaacaaggg gcagctgcct caggcaaatg gtacttaggt gattttaaag accttaatga 180
gatcaggtaa ttgcttggtg aagctaaaaa ttttaccttc aattaactga tcaacagcac 240
catacatcat cataacttct cactcccttc cacctccgta ccatcaagtc atttcctctr 300
gacgtctgaa cctgcactca gggtccattt tttaaacatt tattgaatta gtctggtcta 360
atctacaatt aatggaaagt ctgtagtaat gcagtttcca taagcagcta aggaaagttg 420
aaactttgaa agagcatgta aagtaaacac tttctcttcc ttctacactt ctttgcaatt 480
cataaaatac tttcagaaat catttaaaaa atttggattt cctgcttcca ttttctgaat 540
agatgatgct gataaaaata gtagtgatca cagcccatcc caaatagtgt cattttttt 599
<210>6
<211>599
<212>DNA
<213>Homo sapiens
<400>6
gtcaatgtac aaatgttata ataaatagaa ataatgcaga aaaatgtgta gggtgagaaa 60
atcagaaagt aaaacacatt ctttggttgg caaaagtcag gaataaaagg atactattga 120
acactgacaa agtagatggt aaatgtttaa acaggcaatg gaaaccggat acagtggctc 180
atgcctataa tcccaacact ttgggaggcc agggcaggag gagggttgct tgaggccagg 240
agtttgaggc cagccttggc aacatagtga gacccagtct ttacaatcaa tcaatcaats 300
aataaatcta gccatgtgtg gtgacacact cccgtagtca tagatactag ggaggctgaa 360
gtgggaagat cccttgagcc caggagtttc aggttacaat gaactacaac catgcctcat 420
gacactgcac tccagactgg gtatgaggaa gaccctgtct caaaaacaaa aacaaaaaca 480
taggcaatga aactacaggc atttagaagt tataaatgaa aagttaacca ctcaaaatat 540
gcaacttcct aaataacact ttatttattt aaagaaaaaa acacatgtca caaagaaac 599
<210>7
<211>599
<212>DNA
<213>Homo sapiens
<400>7
attaagactg tccagtatat gaaaccttca aacttgagaa tatataaaca gtacagacat 60
tatggaccat agtactcact caggaaacta attttttatg tgatctcaca tcatccaagt 120
gcatgactca gttcagtcaa atgcccagaa tataaatgga agcaaggcta gtagtaacag 180
tgacagagca agttgttata agaagaactt ccaattatga agcatccaaa aatggaaatt 240
tctgcctctt caagcaggaa gtaaatcaaa tttgaaaata ttagggaaaa aaagcctggr 300
agtcttcaaa tgagatacat actttggatg agggatttgc caagatggcc attgtgattc 360
tagagcgtta aacaaattgt accttaaaat tgtataatgt agacagtcgt gtgtgggcca 420
agtaacttaa tgaaagacaa atagagtttc ctttttcttt gtgtcattga gaaaaatata 480
tatattttaa atttacttct aggaatattt actaattgaa aataattcat aaatggagtc 540
aaatagagtt gacaatgaat gaataaccaa gagtagatgg aaagagatta gtaaagata 599
<210>8
<211>599
<212>DNA
<213>Homo sapiens
<400>8
ggtaatgctg tatattgcaa aatcacagtt tatacttgaa tcactagaat ttttgactca 60
ttttggtgaa cttgattcac taaggttgga aaattacatt ttttaaaatg gtggaagaca 120
attgggtaaa aatgcttgtg ttgtgaagtt agatatttct ctgttttctt ataagtaaaa 180
tcaagaattt caattagtag acattttaaa cacttgctat gtctaacact gtgtgggttg 240
ttaattattt tttttaatgt accagtagtt tcttttctta aggaatttat tattttgtay 300
gcaacctagc tatggctgag gactccataa gaactgagat tttaagagaa cttggctaag 360
tatttaatct gtattgtgag gttcaaacca acttgggttt catcatatgt atgcatttcg 420
attattaagt tgttaaatat tttggagagt tttgctcttt agttttgctt ttaagatgca 480
acagaaaagt caatgtgtta catatgtttt tgatatgtgt ttgataaatt tagaggaatt 540
aaattataca tggcatgatt gcttaaggaa atttaaaatt ttcagcttga cttgatcaa 599
<210>9
<211>599
<212>DNA
<213>Homo sapiens
<400>9
tctgtacctc cacttcctca tctggtaaat gggataataa ttcttgaatc tcaacattat 60
atgtatgaat caaatcagac aatatagaaa aaatcttagc acttaagttc tggcaataag 120
caatgaaaat gttagacttc tatactactt aaaagaagag gtaaaatgat tatcactggt 180
ttcactcaga acaacaaaat ataatttttc tctacctgta atagagcaaa ctaaatacat 240
ttttctctac cagtaatagg caaaaataaa taactaaata aataagctta tgtcaccaay 300
ttcttaatgt attgctttat ttctcttctt ttgacttatg cacgcaaaca tagcccttga 360
catatttaat gtgaaaaatg atttagaatg gtgaggatag gtattttgac accattgcca 420
atttgtgact ctgatatgtt aatttgattc gaatatgtaa acttagcacg tgtctgaaga 480
aatagtgaga tgggggatat tgattaggct tccttgaata aattctgcta gggatacccc 540
ataccaatcc ttgtgtctct tattttaatg actgagcaaa agtcatgact cttctctct 599
<210>10
<211>599
<212>DNA
<213>Homo sapiens
<400>10
aacaatacag aaactgaccc cagattcatc actttacaac cctttcaaaa tatccaaata 60
aataaaatgt ctccatttac tcaaatgttt agttttaaaa tacagattgt attgaaaaag 120
tctgatatat tattttctcc atagtacatg ttgcaaacta atttttttta gcaaaaatag 180
ctaacactta gtgaatatat actttattct aagcactctc caagctgcct tctcacttaa 240
tcttcacatg aacccaatta gtacatacta ttgttattaa ggttgttaaa atgggaaaar 300
taaggtgtgg aaaggttaac atgacatacc tagggtttca tggctaatat gaggatgagt 360
cagattagaa actggtaagt atggtattac aacctatcat ttattatttc attaacgtcg 420
tcacgcttac catgctggct aaattaagtt atctgaaaga ctattttgtt cttataagag 480
tgttggcgct attaggcaag aatattttct tcctatgtaa gactgagctg gaaaaagggg 540
attcagaact gtaatcatcg aggaaacttc ttttcctagg ttatagggaa agaggaaca 599
<210>11
<211>599
<212>DNA
<213>Homo sapiens
<400>11
gcatgagctt taggatcagg ccttcagcag aggaaggaga agttttgctt gctggtttct 60
atgcatattt atccagagtt catcccatga gacattagcc ctcttagagt tccaggatga 120
aaaagagtga tcacaaaatt ttctgccatg tcacagaaaa gcccagggat gggccacagg 180
aatgctggat gggcttgatg tatggtggtg ccaagatgca cctgcaggaa gccatttggg 240
gtccatgcac aggcccatca ctgaagctgt ggccaaaatc agagaacaaa ggggtttagy 300
ggatctgata tgatgcatga acaatgtcta acatgggtgc tttcctgggt tcactggagg 360
agattcaact tgggccctcc aactcaaatt ctaggtgtgt gccctgcctc ctcttcagac 420
atttgccttc tgaaagcctg gcccttggga ggctcacttt atagcacata tcacctctca 480
tatattggag tcccattttc ctcatcctga tagtcctttt gagcaggact tcaatagctc 540
cagtccagtt cactctgcaa caaaagtctg cattgggtcc tagaaaaaca gtgacatat 599
<210>12
<211>599
<212>DNA
<213>Homo sapiens
<400>12
tatgttgcac aggctagttt ccaactcttg gactcaagca atcctcctgc ctcagtctgg 60
gagtcacagg atttacttat ttaaaaactc aattgtttaa gggaaagagt taaactctag 120
tgtgcttcat tgatttcctc tgcccctttt gctgttttat gacaattcct aatttgttca 180
ttaatatttc tagaaagatg ctatgttgtt gccatataat aatatcgata atagtttggc 240
tggttgactg taagtacaaa taacatattc attttcctta aaagtctgtt agtttaaccr 300
tgtatctgac tggccagata gtcttgtcta tttggcttct gggaacggtg tcttcccagt 360
gtcaacgttc ctatctgcct tatttatgtg acctgactga ccagaaatcc taatgtagcc 420
atcttgctcc aaaaggttct tggtcttccc taccttgtta cattacactc atcgtcttct 480
tgacttactg tacactgttt gctctctttt ctccctctct tcttggtgag caactcaaga 540
gatgcctcaa aggttgagag aagtgagctg ggcatggtgg ctcacacctg taatcccag 599
<210>13
<211>599
<212>DNA
<213>Homo sapiens
<400>13
gtgttatttt ttgacttttt aataaaagtt attctgactg gcgtgagatg gtatctcatt 60
gtggttttga tttgtatttc tctaatgatc agtgatactg agcttttctt catatgcttg 120
gtgactgcat gtatgtcttt ttttgaaaaa gtctgttttt taataactag tgagatttct 180
cacttcatca caatattttt taatttagat tttctcattt aaatttctta ccacttttta 240
gacatagatg cattctttat atttcatctg ttttcagatg aatattaatt gacaatttgr 300
tactagatcc tcctttaatt ttttgttcat gacttgtctg tccacagtaa aaaaaaaaag 360
tataaatagt agcaaaatat agaaaccaat ggttttcagg cattggataa caggaaattc 420
aagtctacaa tttttttttt ttttttgaga cagagtcttg ctctgtcgct caggctggag 480
ttcagtggcg caaccttggc tcacttcatc tctgcctcct gggttcaagc aattctcctg 540
cctcagcctc ccaagtagct tggattacaa gtgtatgcca ccatgcccgg ctaattttt 599
<210>14
<211>599
<212>DNA
<213>Homo sapiens
<400>14
acccaagcat aattgatata ggaaccccat taggtgcttt tagcattact gccacctgag 60
caactcatcc tgaatttcag caggactatt tgtcttttta atcaaggtga agaggttaaa 120
tagtctctta aatgactttt gcatacaacc cataacagcc ttgcactgag ataacgtgat 180
aaaaatattt atttccatag aaacagaacc atgtccatac ctgttttagt ctcctccatt 240
tagcgcgtcg attctgaaac caggttttga cctacaggaa agagagaaag aaaacttgcm 300
acgagcccgc gtcgggacgt gccagagggc agggtggctc ctcgcctctc tctcggtggc 360
ccggcgaccc ctaccccaga agccttccct tccccggagg cggccccagg ccccccgcga 420
gctcacctgt ctctcgctga gctgcagcat cttggccaga cgcttcctct cgggcggaga 480
gagatatttc tgcgtctcga atttcttctc cagctcgatg gtctggtcgt tggagaatct 540
cacctggccg cctttccttt tatgcagagg cctctgcaag aaggggctcc agagtagag 599
<210>15
<211>599
<212>DNA
<213>Homo sapiens
<400>15
gaaaaaagtt ctagagatgg atgatggtga tggttacaca acaacgtgga tgtatttact 60
gccaccgaac aatacactta aaaatgctca aaatgggcca ggtgcagtgg ctcatgcttg 120
ttaccccagt actttggaaa gccaaggtag gaagatcact tgaggccagg agtttgagac 180
cagtctgggc aacatagtca gaccccatct atacacacac acaaaaaggt caaaatgata 240
aattttatgt tacatattct ttatcacaat aataaaacca accatgatgg gggggtgggr 300
tgagaaacaa tcaatattct aacaaactaa cctaacaact ataaatgagt aacataatca 360
cattgaagga gtgtaaaaga aaagaactta actatggaaa gctggatttt gactggatac 420
aataaagcct aagagaaaaa gaactacatg caaatattgt gacctatttt aaaaatgttt 480
ctcacaggtg agctagcaat tatgaaacta tgtgtacact agaattgagg aaataagaaa 540
gtaaattgta gaaaatgaaa tccagtgttc tcattgttgg aaaaagatgt tataaataa 599
<210>16
<211>599
<212>DNA
<213>Homo sapiens
<400>16
gcttcctcaa gttcatggga tgcctctgta gctcatggac ccgtggctcc tggtgtccac 60
catgggaatc ttggtaggta gaaaaagagg cccttcctca aggctggtag agccccaggt 120
ggggccaccc cttggcaaat ggggcacaag caggatttca gctccagcca gctcttcaac 180
cagatgtctt tgtgcagtgc acaaattgca caactgtaca cagaagacct gtgtagctat 240
cagagtaatt ttgaattaca catcttgtcc cttcctttgt ccagtcagag atgaaagcgr 300
tactgctcca gggtagtcag gaaacagagg gcaaggaaaa aagtagaggg gagtgtcaga 360
gaagaaacct ctaaatagtg gaggtgacta ttttgaaccc tcagcgtcag ctgctccatc 420
ttactttgct cattgtaaac ctatggtctc agttggaaag ccaagaggat gtttatatag 480
ggtggatcca accacaaact atctgaatta ctgttggaga acctgactct actgtgctcc 540
ataagagtag agacaatatt tatttcattc actgttgtct ccccagctct taacatggt 599
<210>17
<211>599
<212>DNA
<213>Homo sapiens
<400>17
gatttggacc actatgctta aagctaatca gcaagtgatt ctgactagca gaacaccttt 60
gaatttgagc aaacgtactg tggttccaat tgttgtcact cagaggtaca agaagagttg 120
aattaattct cagtggcctg agtaaagatc tagaggcaac cactcacata ttttaaaata 180
ataataataa ttctcttgga attcttccag gtctgtttta ttaacttcct actttctata 240
ctaaaagaaa aaaatagatt agtaatattg ttaacctgat gatgatagaa gacttactcy 300
ttttaaacaa attttcaccc tttctaacag tttagctgaa catcctgaag caagcgaggg 360
aaaatataaa gcattgcaaa ctcaaaagca catataaatc aagtcactta aaataatgga 420
ctcaagaaaa aaaagaaaac atgatattcc tggtgaagct tttgttttca caacaaaatt 480
ttccctggtt acagaaaaat atttctgaag tataaaaaag tgaaggcata aacatgataa 540
tgaatgacaa ctggcatctt cattatgggg atagaagaga ttggtgtggt ctgtggcaa 599
<210>18
<211>599
<212>DNA
<213>Homo sapiens
<400>18
agaacttttt ctgtgtctct ttcttattag attttaagag tttgaacttg aggttatctg 60
catgtcatag aaaacctaaa aagtagaggc ttacatgact taggggattt atttttcgta 120
catattgcaa agtccacagt taggaaattg ctggctttgc tttaccagtt taagggtgtg 180
aggattgaag tcggttgttt taaaggtctt tcccttttgg tgtcaaatgg ttattgctct 240
ctggccatca catgcacttt cccccacaaa aaagatggaa gtaccaaact ggatgtgccr 300
gctgagtctg tcccttcttt aaaggagctt tataggaagt cccgctcaaa acccgtgctt 360
gcatctcatt gtgtggagtc atatcacagg actacctcta tttgtaagag aggctgggaa 420
atgtagtgtt tttttttctt ttgtttttat actgcacaca ttgttgctcc caacagaatt 480
gggattctgt tagtaaggga gatggggcta atgtttattg gcttgatatc tctgctatag 540
ggtataaact ttgggacaag atacacattt tatttatctt tctatcattt atcaggtcc 599
<210>19
<211>599
<212>DNA
<213>Homo sapiens
<400>19
tggtgtgatc ttggctcact gcaacctcca cctccagggt tcaagcgatt ctcctgcctc 60
agcctcccaa gtagctggga ttacagtcac gtgccatcac acccagctaa tttttgtatt 120
tttagtagag atggggtttc gccatgttgg ccaggatggt ctcgaacccc tgacctcagg 180
tgatctacct gccttggcct cccaaagtgc tgggattacg ggcgtaagcc tccgtgccca 240
gccgagaaat gttgattatt aggctgagta gtttgaactc aacaaaggca atgtaaaatr 300
acatttcagg gactgcttag tgcaacaaaa cacttacatg gggccattag accctaacta 360
tctctattag acttagccct gagggcaagt acaataattc agcctgtata ttcactgcca 420
ggcccacagt aagcatttca agaagagtat gagaaaagtg gatgtgacca ggcatggtgg 480
ctcatgcctg taatctcagc actttgggag gctgaggtgg gtggatcacg aggtcaggag 540
ttcgagacca tcctggccaa catggcgaaa cctcgtctct actaaaaata caaaaatta 599
<210>20
<211>599
<212>DNA
<213>Homo sapiens
<400>20
tgaggcttta aaccaaaacc aagccttcaa accataatct gtaaatatca gatatctgaa 60
aaacagcttc tggtattctt aagactttaa taatgactgt ctaaagtttt attaaatgag 120
cttattataa tatgacagaa ctcttataat agttaacatt tattgagcaa ttcactgtgt 180
ttattcctcc catcaatata gatataaatt ctattactag tcagattttt aatgaggaaa 240
ctgagaccct gtgagcatct aatagtatgt agatcatctt gcagaaggtg gtagtgatcr 300
tactacctga aaagcatcca tgtttgagtg gctcttttgt gtgttttttg gcaacttaaa 360
actgcagcat tttctcatac atctacatag ggtatttccc ttaaacccgt tgagaacttt 420
ttaggtgtat attctaaggc tgatcccctt tttataaatt tgctgttttg aaatgcttaa 480
aattgttaga cagctcttta aaaaaacaat acaaaaaaat ctgatctgaa aagtatctta 540
gcatgaatgg tttggctttc ctggctttaa ggaagcaagt tcagtatgtg agctatttc 599
<210>21
<211>599
<212>DNA
<213>Homo sapiens
<400>21
agtgttggga ttataggcat gagccactgt atccggtttc cattgcctgt ttaaacattt 60
accatctact ttgtcagtgt tcaatagtat ccttttattc ctgacttttg ccaaccaaag 120
aatgtgtttt actttctgat tttctcaccc tacacatttt tctgcattat ttctatttat 180
tataacattt gtacattgac ctactttttt cctacttttg tgcattgcta caatgcatag 240
catttgtaca ttcatctttg gcttttgaca attaatattc ccccctgtat tttagttttr 300
gatctacagt tatgtagcaa tgagctcatt atcagtcctt ttgctgaaat ttccccagac 360
ctctcttggt tgcatgaagc tcatcctgtt ttatttagat tcttcaagag gggattatgg 420
attcttgtgt gttaaaaact ttttatatag ccttgaaact tgaaggatac cttggttgtt 480
tttaaaattc attattcata cttttattga atttctaaaa aatgcttttc cattgttgca 540
ttatattttg ttttagacaa ctctgatgcc agttgaattt atttgccttc gttcattat 599
<210>22
<211>599
<212>DNA
<213>Homo sapiens
<400>22
gctcttgttg cccaggctgg agtgcaatgg cgcgatctcg gctaaccaaa acctctgcct 60
cccgggttca agcaattctc ctgtctcagc ctcctgagta gctgggatta caggcatgtg 120
ccaccacgcc cggctaattt tgtattttta gtagagacag ggtttctcta tgttggtcag 180
gctggtcatg aactcccaac ctcaggtgat ctgtccgcct tggcctccca aagtgttggg 240
attactggcg tgagccaccg tttccggcaa ttgacagtct taccatgacc acgtggtacr 300
tatgttaact ataatccagc caatccttga tggattgatg tttggtttat ttctaatctt 360
tggttcttac tgacaatata aagctagctg agatttgaac acagattttt cggataccca 420
gtgcataatt tgtcatagct gtaatgaata cattacagag gaaaggagcc tggctggctg 480
ccactctgtt aattgaatga tgtgaatgga aggatgggaa tccagcaaat gagaaggaaa 540
attcatcaga aagctttgag gacaactgaa agaatgtaag atccttaaca ctgcaactc 599
<210>23
<211>599
<212>DNA
<213>Homo sapiens
<400>23
gaggcagaag aattgcttga acctgggagg cagaggttgc agtgagccaa gatctcacca 60
ctgtactctt gcactctagc ctgagcaaca agagcaaaat tccatctcaa aaaaaaaaca 120
aacagaaaaa tacctcttca cagatagctt tcagatgcca catgcaggag ctgtgaaaaa 180
aggggggaaa aatagagcag aaaaataatt tgacaaaatt caacagcctt tcacggtaaa 240
aactctcaac aaattaggta tacaaggaat acctccattg tgtacttcag tacaataagr 300
gccatggaag acaagcaaac aatgaacgta ttcagcagtg aaaagctgaa agcctttcct 360
ctataatcag gaacaagcat gacaaggatg cccactctca tttctattca tcaatgataa 420
ttttctgatt ctgatggtat tgtgatgata tagaaaatca cactaaagag gccgggcaca 480
gtggctcacg cctgtaatcc caacactttg ggagctgaga tggggggatc accagaggcc 540
aggagttcaa gaccagcctg accaacatgg tgaaatccca tctctactaa aaatacaga 599
<210>24
<211>599
<212>DNA
<213>Homo sapiens
<400>24
agagagaaag tgaagaggaa agcactgcac acttttaaac aaccagatct tgtgagaact 60
cattcactat catgagaaca gcaaggggga agtccatctt tatcactcgg ttatctccca 120
tcaggtccct cctccaacat gtggggatta taattcaaca tgagatttca gtggggacag 180
agaaccagac catatcaaga tgtgtatata gtagttctat gccattttgt cacttgtata 240
gatttgtgta accaccactg caatcaagat acagaactat cctatcatca caaggatcty 300
tcttgctaat tcactgtagt cacactcacc tcatcttttc catgattcct aacccctggc 360
aaccactaat ctgttcactt tttaaagccc tggagtaatt tgttcaaagg aaagctttta 420
ttgaggccca ttgtataaaa caacaataat aacagagaaa acaaggggaa gaaggcaagt 480
gggatgctaa ggactataac ttgaaaattc ctgattgtgt ttatccttga agatattagg 540
aagcaagact ttcacagagc attttttaaa agttaatagt gataaaagat attagacct 599
<210>25
<211>599
<212>DNA
<213>Homo sapiens
<400>25
atttatttct tttctttctt ttctttcttt ctttctcatt ctgttatcca gactgaagtg 60
cagtggcaca aacttgcctc actgcagcct cgacctccca ggctgaatct atcctctgct 120
ttctgagtag ctgggaccac aggtgtgtgc caccacagcc agctaatttt tgtatttttt 180
gtagaaatga ggtctcacta tgttgcccag gccaatctca aattcctggg gtcaagaatt 240
ccacccgcct cgccctccca aactgctgag attacaggcg tgagccacta cccccagccy 300
ggtcttgttt tcagtggtaa attagtacac gtgttttccc tcaacattgg ggtgataaaa 360
tagctctggt atatattttt accatttaag tttttattca ccacctttaa agttttccta 420
tttcttttct ccatttagtt aataaattaa gtagactatt ctggtaagtg cctctgaaat 480
caaaaagctt tcaagttttt caaaactgca agttggccgg gcgcggtggc tcacgcctgt 540
aatcccagca ctttgggagg ccgaggcggg tggatcatga ggtcaggaga tcgagacca 599
<210>26
<211>599
<212>DNA
<213>Homo sapiens
<400>26
gctattatag ggcaacttac cgagatggaa attatcaatg aattttaaat gtatgtctaa 60
ccaacattaa tattatatca agtttatgtt acattgttct atatgtataa tttgtcacac 120
cccaatataa ttttaattat tagaaagctt tatagctaat ttaaacataa catttgtaat 180
gttcatgatt tgttttacat actaggcaag ggaaactgct taataacaga ataaatgact 240
gattgtccaa tttagggact agacaagagg ctgtagtaga tgccctgaag taacattgcy 300
tagtaagagg aataaaacaa gtagacagat tttgaaagca tcataactgg aggagggttg 360
tgaaaggttt catgagcaaa gatgatcctt tcaaaggtca tttaatggca atgctgtgaa 420
aagacagtgg ccaaggcatc cattctcaat gcagaagatt tactaagatc acgggccctg 480
gagccagact gtccacgttc agatctgcca cttaagagct gagtgatctt gggaagttat 540
atatcctttg agtcttaatt ccccatttca acagtgtcat tctcttttcc tataggaaa 599
<210>27
<211>599
<212>DNA
<213>Homo sapiens
<400>27
cttgtataac cgttgacaaa attaattagc aacgctaaaa ccaatgaaaa cacattactc 60
ttagttctta gagtattaaa gaattcagtg ttgactttca aactattatc tttgccttat 120
ttgttaatgt attgtaattg aaactaatta attttaaatc taagctttaa tcatgtaaca 180
taacatttcc tagagtgtgt tccatgggat attgatgagc attatgtagg tttctgtgat 240
caaataagtt tgggaatatt ttagaaaata ataatatcac taacactgta ttgattcctr 300
ttgtctttcc catttccact gctgctgcct taggcaaggc tttcattagc acttccctga 360
gatgttaccc ccaccctacc tccaactgtc cccatctctg tcaattcact ttagttcaac 420
tggactcatc agttgcctac tgtgtaatca ctggtacctg tgatctcacc aaaagtatct 480
gtcctaaaac tcgttttaca tttgtagttc tcaactgggg gcgattgtac cctccaggag 540
acttggcaat gtctgtagac attcgtggtt gtcacaactc aggctggagc aagagatgt 599
<210>28
<211>599
<212>DNA
<213>Homo sapiens
<400>28
agaacagtac agtcaagggg gcaggtcttg cagtaagcct gacggggttt gaatcctagc 60
tgtatgacct taggagagtt gcagagcttc tctgagtttc agattccttc cgttataaag 120
caaggatagg aatagtatct actgcaaaag gttgttgcag aagtccaata agagaaggca 180
cacatagtgc ttagaatagt gcctggtaca tagaaaatgc tcactgaacc ttggctatta 240
ttttccagaa atgtttggag atttttatag cataggcaag aaactttgtg gcactggttr 300
tttaccattt taaaacgtaa aaaaaaagga aatatttgat ttgattaata gctgaactga 360
caacgtgcaa gaaccattta ataatgacca atcatgaaag gttatggaaa aagaaatgat 420
ttaagaaggc caagagccag gccaaggtat tggtaagccc tgaactcatc acatgtctgg 480
agtcgacagt tcaatcagtt ctagttttga atatgggcag gagctttttg taaaaaaaaa 540
gttttggggc aatatgcata tggctttctt ttctttcttt ctcttttcct gccccctgg 599
<210>29
<211>599
<212>DNA
<213>Homo sapiens
<400>29
tcttttgtct ttttgataac agctatgtag gatattctga aaatatattt aaaatattct 60
tatttttcct agtcagtatt tcctgacttt tctcctctag gttgcatgta ttgctttagt 120
aataaacaaa agcattaatg aaaacttcgt tttgaaaaaa ttggatctgg gatagaattt 180
accattttgt tgtgggttta ttttctgata tggatttatt ttactatttc acttgtaatt 240
ttagtttttc tcaacttagg taagaagacg aatgagaagg tgagagacag ctagtgcgtr 300
ctcatgggat atgacatttg aagggaaaag gatttgatag aatctcaggc atttgataaa 360
gagttatttg gcacttgcag taagctaagc actgttctgg tttggtgcac actgtgatga 420
ataagagagt ttctcagcct tgtggggatt ggggagatag gaactaacta aatattttca 480
aaataaaaac aggatttcag gtttgatagg cttgataata agcatgcagc ataaagtaaa 540
aaggagtgat gaggtcaaat ggggcctatt ttatataata tggtcaggga aaccatttg 599
<210>30
<211>599
<212>DNA
<213>Homo sapiens
<400>30
ataaaataaa ataaagaaaa gtaaaaaatg tagctgtgag gtgatgggca tgttaatttg 60
cttgactatg gtaatcacct tggcatgtat atgtacctca agataagtgt agcaaaacgt 120
gttacacatc ttaaatatgt acaatttaaa aacaagacag aggctcagga aggagaaata 180
actcgctcaa ggtcacatag ctagtaagtg gcagagcctg agttcaaaac tgagcagctg 240
cctccaggtt gggcttgtca ccactatttc atgtaccctc taaattcttg tggggaaatm 300
agggtgctta ttcaatccat gctgcatata gaggttgtta gatggcgaat acttcttgtc 360
cattttttgt ttcaaagatg gggtttcccc caaatacccc tggcctctgt ctcaccttta 420
ctcaccctct ggggagatga gcgagttgct tgagtgcgag aagaaagggc aggcatggcc 480
tagtctttta actacacatc ttgttctgct cctccagggg caggtcttcc tagttgggtg 540
aggttggggg aggccttcag agttctagtg ctccagagta tgtgagtctg cctatcttt 599
<210>31
<211>599
<212>DNA
<213>Homo sapiens
<400>31
taagcatgta tattatgtat attaaaaagt tacgaatcat tttgttctaa atcattacag 60
cattttctct ggaatatagc attttcagaa ccacaatgct cattgaagga attgaagaaa 120
aataattata attttattct tagcatttat aattgattta ataaaagcaa catgagaata 180
atttaagaat gagtaaaaac aggggttttg tttgtgagta gcagagggtc cctaataata 240
gggtattaac aatgaaaatg tatttctctt ttacttaagc aaaagccaga gataggtagm 300
tgaattcatc ctcagtttca tattcgcttt ttagccatga ggacagagcc agaggttggg 360
aagggtacac ggctcttaac ccttcaagga tactttttga cattgtcata ctacttctac 420
tcacatccca ttaattagaa ataagtaata tgtgtggcta acaatcatag gaaaaaaagc 480
taaacatcac taattattag agtaatgcaa atcaaaatca caacgagata ccatctcata 540
ccaaccagaa tggctatcat taaaaagtca aaaaataaca gatgttggtg agattgtgg 599
<210>32
<211>599
<212>DNA
<213>Homo sapiens
<400>32
tttccagaat ggatgatgac tcttgctaaa gttcttaatg acacaaagta tgttcttcct 60
tccattttca cagaggttgg attcctgaaa ctgtacaaga aatacttaaa ataagtcctc 120
acttaatgtc aatgataagt tcttggaaac tataattgaa acgatgtata gagagatgag 180
tttttttttt tctaagcaat gttataaaga aactgtcgaa tgaaacaatt ttattccagg 240
agctactata tgtaaaatgg agataggttc taaggagata ttataaatat attttttacr 300
tatatgcatg ttaagtgggg ctttcagaac tcatgcaggt cgtgtggaat tctctgtaca 360
gcatagatct gtgtagcgga agacatctag cacctctgac ttcggcctgc ttaatggcag 420
ttgcaccccc ccaattagta ttatatccaa aaagatttgg atttaaccac ttactgatta 480
aaatttctat atggcctgaa gcacacaaaa caagataaaa gaaaaacagg agaatatgga 540
tgtgaaggta tatatgacat tatgtttata agtttaaata aatactatta aagagctcc 599
<210>33
<211>599
<212>DNA
<213>Homo sapiens
<400>33
ctcctccaac aacgggaatt acaatttgac atgagatttt ggtggggaca cagagtcaaa 60
tcatatcaca acccaagtta gatttcccaa cttgaattcc tttaaagcat ctgagattta 120
ctatagctgt gctattctat tagactgcat tataattttt agttggcttg tgtttttcct 180
tctgaactgt gagctctttt taatttcaat atcactagtg ctaagtaaca taacctctca 240
gatgcttact gaattgattt aaatcttcta tttacttttt ccttccactt aaaatagtar 300
ctcttttctt tttctttctt tttttttttt ttttttagag acagggtctt gctatgttgc 360
ctaggcagga gtgcagtgac tttcacaggt gcaagcatag caccctgtag ccttgaactc 420
ctgggctcaa gaagtcctcc agcctcagcc tcctgagtag ctggaactac aggtgcacac 480
actgtgcctg tcttatattt tattgagtaa tgtgttaggt acttttatat actttgtcta 540
gtttaatccc actggagctt aggaaacaag tttagaggag ttaagtatct tgcccccat 599
<210>34
<211>599
<212>DNA
<213>Homo sapiens
<400>34
tttttatttt cttgacaata tcttttgcag agcaaaattt tataatttta atgaaatcca 60
gtttattgat tcttgtgttc atggagtgtg ccttttgtgt aatatctaaa aagtcattgt 120
caaacctaag ggcatctaga ttttctccta tgttatctcc caggagttct gtagttttat 180
gttttacatt gaggtctgag ccatttcgat ctaatttttg tcaagaatgt aaggtctgtg 240
tttagatttg tttttttagc atgtggatgt ccagttgttc taccaccgtt ttttgaagay 300
ggtctttgta ccatcatatt gctttcgctc cttggtcaaa gatccgatga ttgtattttt 360
gtgggtctat ttctgggctc tttgttctgt tccattgatc tgtttgtctg ttctttcatc 420
agtagtacat tgtcttgatt attgttgctt tgcagaaagc cttaaagttg agtagtgtca 480
tccttctgac tttattcttc tcttttaata ttatgttggc tattctaggt cttttgcctt 540
gccatgtaag ctttctaatc actttgttga tatccacaaa ataacttgct gggattttg 599
<210>35
<211>599
<212>DNA
<213>Homo sapiens
<400>35
ctgtcactgt ctcccatcac ccccagatgg gaccttgtag ttgcaagaaa acaagctcag 60
ggctcgcact gattttatat tatggtgagt tgtgtaacta tttcattata tattacaatg 120
taataataga aataaagtgc acaataaatg taatgcgctt gaatcattct gaaaccatct 180
cccacccaat ctgtggaaaa attacctttc cctcgtgcca aaacggttgg gggccactga 240
actaggacat ccagtatgat attgaaaagg agtggtgagg ggacattttt gtctttgttm 300
gtgattttag tgggaaagct tcgagtttct caccgttaag tatgatgtta gctatagatt 360
tttgtagtct ttttaactag ttgaagttac tctatatctc gttcactgag atcttttatc 420
atgaatgggt tttggatttt gtcaagtgtg tctgtcaata gatgcagaaa aatctattgc 480
tatgattatg tgatttttct tctttagcct gttgatgtga tgaattacat taattgattt 540
tcaaatgttg aaccagccat gcaatcagag gtaaatccca gttagtcatg gtgtataat 599
<210>36
<211>599
<212>DNA
<213>Homo sapiens
<400>36
ctggagtgca tggtcacagc tcactgcagc ctcgacctcc taggcacggg tgatcctcct 60
gagtaactgg gactgtaggc actcaccacc acatgcagct aatttttcta tttttggtag 120
agaggggatt ttgccatgtt gcccaggctg gtcttgagct cctgggctca agtgatctgt 180
ctgcctctgc ctaccatagt gctaggatta taggcatgag ccaccttgcc ctgcttcttt 240
ctctagatcc aaatttctga cctacatcat ttttatccta tgtaaaggat tttttttttw 300
aatttcttgc aaggtagatc tactggcaaa aaaatctctc aatttttgtt tgtttgagaa 360
agtatttttc cttcactttt gaagaatgat tttgcgggtt acagaattct atgttgttgt 420
tttttctttt tttttatcaa cactttattt tactttacca ctcgcttgta tggtttctag 480
cagaagtaaa atgtaattct tatctttgct tctgtctagc taagggttat tcccccctca 540
ccccccacct ctggcttctt tcaggttttt ttctttatct ttgactttct gtagtttgc 599
<210>37
<211>599
<212>DNA
<213>Homo sapiens
<400>37
tgtagatggt taatatccaa aaaacaaaaa ggaaaaaaaa acccaactct tttatgtgct 60
ctaatgtatt tgctgttgaa aacaaaggga atttgtgatt catttaaaaa atatatatat 120
agctatttga tgattttgaa gatattttat agttattgtt ccatttgaat gaagtaaaaa 180
tgttatgatg tcttagggta caattgagct ataattttag tatgtcaatg actacagctt 240
ttttctaggg aaggttataa tagttaacag gaaaatatga cataaatagt aaaaacatgr 300
tataatagta aaaattagta aaaaattttt gttttgggct gtaagttttt ttggagagga 360
gactgtagta agtgtaaaaa catgatgtta tataattagt tttacacata ttttcaggaa 420
tatttttaat acacaaatgt gtggatatag tctattttgg tgtgaattta aactttgaaa 480
actgctaaaa gtttcagtta cttgagaaat agtgaatttt tatttaatga tcaaattaaa 540
cactaataaa attatgtctt tattgttttc agttttgaga gcatttgaaa agtgcaggc 599
<210>38
<211>599
<212>DNA
<213>Homo sapiens
<400>38
ttagcagcag aattacaaga aatcttggga cctgtagtct taattcaaga taaggccatg 60
gggcctgagg cacccctaga ccctgagcct tcaccgttag gatgggcatc cccccgttcc 120
taatagggta tggtatacag ataggtctag ccggggtgct tctgctgcct gactgttgtt 180
gcagcccagc ctagtactaa caccatatgg tatgaaacca ggtgtaaaca gagtagctaa 240
tgagctaact cagggcagtg tgaataataa ccaccaaaag ggtgacacct gtggtaatcy 300
gcaccaatag ctgggcagtt tatcgaggct tatgtatgtc atgggcctgt gtgcctaaag 360
cctacgtgtc aagctactgt gtcaagcctg tgtgtgtata tctggcctgt atgcccaaag 420
cctacggtca ggctaatgtg tgtcaaacct gtgtatccaa aacctacgtc tccctcggcc 480
tgggggggtg gagtgtaagg catatggacg tgctttggtc aaggaatagg ctgaggtgga 540
tatccaggcc tgcatgagtc cagcgagttt ggtgtgcagg cgcacacctc cacttgtta 599
<210>39
<211>599
<212>DNA
<213>Homo sapiens
<400>39
gctcagcctc ctgagtagct gacaaccgca ggcacacacc accatgccca actaattttt 60
gtcctggcta cttttactcc taaaccttct ccttctccat aaacatcata tttttcataa 120
cttctcccct tccactcttt ataccagcaa gacgaatgtt gccccagtgg ccaaatatgt 180
gttatttcac attgccctgc tctaattaag gaggtgtgaa gaaggaaatg ctagcaacag 240
caatgataac tcaggtggct ttgtgtgtgt gttctgggag tggagtgtct ttaagtgagm 300
atattggagc acaggataga caggaagagc tccagtgtta gtaaaattat gaatcattac 360
tatgttgggg acttctagaa tttaggtcca aaacaattct cctgtggttg caatccatta 420
taagtcattc aattaataaa gaatgaacca attaatggaa ataatataaa ccatagactt 480
ctgaaaaagt ggtaaggatt tttgaaatat atcctaatgt gcaccaggag tcaagtaaca 540
tggacagagt atttactcag cacataagtt gttaggcaca gattattaag actaaagaa 599
<210>40
<211>599
<212>DNA
<213>Homo sapiens
<400>40
gatggggact ttgccaaatt gagagctccc gttagttttt cttcttggtt ttatgagccc 60
atgacccatc ttgattcttt cttgcctgat gaggttttga tagactatag attggcttac 120
ctgacctaga aacttgtgat ttttactctg gtagatatcc ataccaacct aaggggccta 180
aatccatgcc aacccttgat gccctgatac tggatcctac ataaaaagag agtttgaacc 240
cagtatttct aagatcctga tgtttcaaaa acaatgctac tttatcttat gaatcatttr 300
tcacttgctc cttcaggcag gtaaatataa tgaggctgac agcagccaag gccatgctgc 360
tcatcataca caatcatgtc aacctgctgt gaaagtctgt gccattttcc ctccatcaca 420
tctgtcttgt tctctttgcc gaaagctgag ttactacatc ttggctgatt cagaattttg 480
tctagttctt tccttacagg ttccccattg tctctttgat actattttat acctaaaatt 540
cctctctatt tgtttaatgc aaatcagaaa cctaaggcaa gggacagctg gtcaccaaa 599
<210>41
<211>599
<212>DNA
<213>Homo sapiens
<400>41
tatatattta cagggcacat gaggtgtttc gatacaggcc tccagtgcat aataatcaca 60
tcatcgagaa tggaatatcc attcccttgt gcatctatcc tttgtgttac aaataattca 120
attacactgt ttaagttgct ttaaaatggg caatttttat tgactatagt tatcctgctg 180
tgctatcaga tagtaagtct tatttattct ttctgtgttt tttgtaccca ttaactatcc 240
ccaccttccc ccaacaccac actatgcttc caaacctctg gtaaacatcc ttctacctay 300
gttcatgagt tcaattgttt tgattttgag atctcacaaa ttagtaagaa catgcaatgt 360
ttatctttct gtgcctggtt tatttcactt aacacaatga cctccacttt catgcatgtt 420
gttgcaagtg acaggacctc attcattttt atggctgaat agtactccat tatgtctatg 480
taccacattt tctttatcca ttcatctgtt gatgaacaca taggttgctt ccaaacctta 540
ggtatggtga acagtgctgc aacaaacata ggagtgcaga tatctctttg atgtactga 599
<210>42
<211>599
<212>DNA
<213>Homo sapiens
<400>42
ggagaataaa tggcaccatg caggaacaag gaagagaagt ggacatgcat tttttttctg 60
tttttttttt tctttttgag acggagtctc gctctgtcgc caggttggag tgcagtggca 120
ccatctctgc tcactgcaac ctctgcctcc caggttcaag caattctccc gcctcagcct 180
cctgagtagc tgggattaca agcatgtgcc accatgccca gctaattttt gtatttttag 240
tagagatgga gtttcgccac gttggccagg attgtctcga cctcttgacc ttgtgattcr 300
cttgccttgg catttattga tccatttata aactattgac cttgtaagaa tgtctacagc 360
agagtgttta atttgtatgt ctattcatta aaactccttt gattgatagt taattggttt 420
ataatattag tttcaagact aaacaagctg tgtatgtaac atttgatctt tcagttacag 480
gctttatcac aaaaaagtaa tgctgtatct ctttttcctg gatttttatc cacctgaaat 540
ttctgatgtc cacaatggtc ccacctggct atctactttc tgcacatatt ttcacgtgt 599
<210>43
<211>599
<212>DNA
<213>Homo sapiens
<400>43
aaaaaaaaag ataaacactt ttacctctag taactgatca caacaaagct gccatttcat 60
accatgggag actccatggt tatccagaat caaaggtctt tcattccctg ccctcttgcc 120
aagccacatt tcaggagtca gcattgttct aatggatgcc atttgtcttt acactatctg 180
aagtgagcca gacttcaagg tttgagagca cagtctccca gactgcaaag tttgcccaag 240
cctctgatac tccctgcaag gaggtcccac aaggttaggg ggcatggttt taggacttcy 300
gtcacctctg scctaactgc aagttcagga agtttccaaa acgaccttca ggttcaaaaa 360
ttcactaaaa agaatcacag aactctttga aagctgttat sctcacagtt acagtttatt 420
tcagggaaag gatagagatc aaaattagcc aaagaaagag attgatagag cagagtctga 480
gagggttcca aatgtgaagc ctctggggtc ctcagaatgc attaccctgc tgatttggat 540
gtgtgagaca acacatatag agtactgcca atcagggaca ctcacctgag cttcagcat 599
<210>44
<211>599
<212>DNA
<213>Homo sapiens
<400>44
acacatattt taattaaata gcttcatatt tagcttcaag aagatgagta ctggccatat 60
atttgaatat atatgctata ttagtttaaa tctcatgtca gatgatttgc aggtcttttt 120
atttgcatgt ttatgcagct aagatgtttg gcgctcagtt cttgtgggag ggggcagctt 180
ggtatggaaa gaacttggag tttgaaatct cactgtcctg gatttgaatc tcagctgtta 240
catggccttg agcaggctac ttatctgatt ttttgaggtg gattcttgtc tgtaagatcr 300
agatagggaa acccaactct gaaagggtta aatgagataa ggcatataaa atatcagtaa 360
caggctcact ggctcacacc tgtaatccca gcactttggg aggccgagag gggagggtcg 420
cttgagccca ggagtttgag accaacctgg gcaacatagc gagaccccgt ttctattaaa 480
aaaaaaaaga aacagaaaaa caaaaacaaa aaaaagaaaa tatcgacaac agtgtctgat 540
gctcagtaag ggctaaacaa aattgagtta ctctaccaag gaaacgacaa tagaatggt 599
Claims (108)
1.在人类个体中确定对2型糖尿病的易感性的方法,包括在从个体获得的核酸样品中确定至少一个多态性标记物的至少一个等位基因的存在或不存在,其中至少一个多态性标记物选自表10-12中显示的标记物、以及与其连锁不平衡的标记物,其中至少一个等位基因的存在或不存在的确定是对2型糖尿病易感性的指示。
2.权利要求1的方法,其中至少一个多态性标记物显示在SEQ IDNO:1、SEQ ID NO:2或SEQ ID NO:3中。
3.权利要求1或2的方法,其中至少一个多态性标记物包括选自下列的至少一个标记物:rs2497304(SEQ ID NO:16)、rs947591(SEQID NO:30)、rs10882091(SEQ ID NO:4)、rs7914814(SEQ ID NO:24)、rs6583830(SEQ ID NO:20)、rs2421943(SEQ ID NO:15)、rs6583826(SEQ ID NO:19)、rs7752906(SEQ ID NO:32)、rs1569699(SEQ IDNO:6)、rs7756992(SEQ ID NO:21)、rs9350271(SEQ ID NO:33)、rs9356744(SEQ ID NO:34)、rs9368222(SEQ ID NO:35)、rs10440833(SEQ ID NO:36)、rs6931514(SEQ ID NO:37)、rs1860316(SEQ IDNO:10)、rs1981647(SEQ ID NO:11)、rs1843622(SEQ ID NO:9)、rs2191113(SEQ ID NO:13)、rs9890889(SEQ ID NO:31),以及与其连锁不平衡的标记物。
4.上述权利要求任一项的方法,其中至少一个多态性标记物包括与选自表22、表23和表24中显示的标记物中的一个或多个标记物强烈连锁不平衡的至少一个标记物,强烈连锁不平衡被定义为数值|D′|大于0.8和/或r2大于0.2。
5.上述权利要求任一项的方法,其中至少一个多态性标记物选自标记物rs7752906(SEQ ID NO:32)、rs1569699(SEQ ID NO:6)、rs7756992(SEQ ID NO:21)、rs9350271(SEQ ID NO:33)、rs9356744(SEQ ID NO:34)、rs9368222(SEQ ID NO:35)、rs10440833(SEQ IDNO:36)、rs6931514(SEQ ID NO:37),以及与其连锁不平衡的标记物。
6.权利要求5的方法,其中至少一个多态性标记物选自标记物rs7752906(SEQ ID NO:32)、rs1569699(SEQ ID NO:6)、rs7756992(SEQ ID NO:21)、rs9350271(SEQ ID NO:33)、rs9356744(SEQ IDNO:34)、rs9368222(SEQ ID NO:35)、rs10440833(SEQ ID NO:36)和rs6931514(SEQ ID NO:37)。
7.权利要求1的方法,其中至少一个标记物选自标记物rs7756992(SEQ ID NO:21)以及与其连锁不平衡的标记物。
8.权利要求7的方法,其中至少一个标记物选自表22中显示的标记物。
9.权利要求1的方法,其中至少一个标记物选自标记物rs10882091(SEQ ID NO:4)以及与其连锁不平衡的标记物。
10.权利要求9的方法,其中至少一个标记物选自表23中显示的标记物。
11.权利要求1的方法,其中至少一个标记物选自标记物rs2191113(SEQ ID NO:13)以及与其连锁不平衡的标记物。
12.权利要求11的方法,其中至少一个标记物选自表24中显示的标记物。
13.上述权利要求任一项的方法,还包括评估个体中至少一种单倍型的频率。
14.上述权利要求任一项的方法,其中在来自个体的核酸样品中至少一个多态性标记物的至少一个风险等位基因的存在,指示了对2型糖尿病的增加的易感性。
15.权利要求14的方法,其中增加的易感性的特征为相对风险(RR)或比值比(OR)为至少1.15。
16.权利要求14或15的方法,其中增加的易感性的特征为相对风险(RR)或比值比(OR)为至少1.20。
17.权利要求14-16任一项的方法,其中rs2497304等位基因A、rs947591等位基因A、rs10882091等位基因C、rs7914814等位基因T、rs6583830等位基因A、rs2421943等位基因G、rs6583826等位基因G、rs7752906等位基因A、rs1569699等位基因C、rs7756992等位基因G、rs9350271等位基因A、rs9356744等位基因C、rs9368222等位基因A、rs10440833等位基因A、rs6931514等位基因G、rs1860316等位基因A、rs1981647等位基因C、rs1843622等位基因T、rs2191113等位基因A和/或rs9890889等位基因A的存在,指示了对2型糖尿病的增加的易感性。
18.权利要求1-13任一项的方法,其中在来自个体的核酸样品中至少一个保护性等位基因的存在,指示了对2型糖尿病的降低的易感性。
19.权利要求1-13任一项的方法,其中来自个体的核酸样品中至少一个风险等位基因的不存在,指示了对2型糖尿病的降低的易感性。
20.上述权利要求任一项的方法,其中至少一个标记物或单倍型进一步与个体中胰岛素响应和/或受损的葡萄糖耐受性相关。
21.权利要求14-17任一项的方法,其中风险标记物的至少一个等位基因或单倍型的存在的确定,指示了对2型糖尿病的增加的易感性,其中至少一个等位基因或单倍型还指示了降低的胰岛素响应和/或受损的葡萄糖耐受性。
22.上述权利要求任一项的方法,其中连锁不平衡的特征为数值|D′|大于0.8和/或r2大于0.2。
23.在人类个体中评估对2型糖尿病的易感性的方法,包括在来自个体的核酸中筛选SEQ ID NO:1、SEQ ID NO:2或SEQ ID NO:3中与人类群体中2型糖尿病的增加的发病率相关的至少一个多态性标记物或单倍型,
其中在核酸中至少一个多态性的风险标记物等位基因或风险单倍型的存在,使个体被鉴定为具有对糖尿病的升高的易感性,
其中在核酸中至少一个风险标记物等位基因或风险单倍型的不存在,使个体被鉴定为不具有升高的易感性。
24.权利要求23的方法,其中多态性或单倍型选自rs2497304(SEQID NO:16)、rs947591(SEQ ID NO:30)、rs10882091(SEQ ID NO:4)、rs7914814(SEQ ID NO:24)、rs6583830(SEQ ID NO:20)、rs2421943(SEQ ID NO:15)、rs6583826(SEQ ID NO:19)、rs7752906(SEQ IDNO:32)、rs1569699(SEQ ID NO:6)、rs7756992(SEQ ID NO:21)、rs9350271(SEQ ID NO:33)、rs9356744(SEQ ID NO:34)、rs9368222(SEQ ID NO:35)、rs10440833(SEQ ID NO:36)、rs6931514(SEQ IDNO:37)、rs1860316(SEQ ID NO:10)、rs1981647(SEQ ID NO:11)、rs1843622(SEQ ID NO:9)、rs2191113(SEQ ID NO:13)、rs9890889(SEQ ID NO:31),以及与其连锁不平衡的标记物,其中连锁不平衡的特征为数值|D′|大于0.8和/或r2大于0.2。
25.前述权利要求任一项的方法,还包括在核酸中筛选与LD区块C06(SEQ ID NO:1)、LD区块C10(SEQ ID NO:2)和LD区块C17(SEQ ID NO:3)不相关的2型糖尿病的至少一个风险遗传变体的存在。
26.权利要求25的方法,包括筛选核酸中TCF7L2基因中的2型糖尿病的至少一个风险变体的至少一个风险等位基因的存在或不存在,其中至少一个风险等位基因的存在的确定,指示了对2型糖尿病的增加的易感性。
26.权利要求25的方法,其中TCF7L2基因中的至少一个风险变体选自标记物DG10S478、rs12255372、rs7895340、rs11196205、rs7901695、rs7903146、rs12243326和rs4506565,以及与其连锁不平衡的标记物。
27.前述权利要求任一项的方法,其中标记物或单倍型的存在,指示了对象对2型糖尿病的具体治疗模式的不同的响应速度。
28.前述权利要求任一项的方法,其中个体具有特定的人类血统。
29.权利要求28的方法,其中血统选自非洲黑人血统、高加索人血统和中国人血统。
30.权利要求28或29的方法,其中血统是非洲黑人血统。
31.权利要求28的方法,其中血统是欧洲人血统。
32.权利要求28或29的方法,其中血统是高加索人血统。
33.权利要求28-32任一项的方法,其中血统是自己报告的。
34.权利要求28-32任一项的方法,其中血统是通过包括在个体的核酸样品中检测至少一个多态性标记物的至少一个等位基因的遗传测定来确定的,其中等位基因的存在或不存在是个体血统的指示。
35.前述权利要求任一项的方法,其中个体是肥胖的。
36.权利要求1-34任一项的方法,其中个体是不肥胖的。
37.用于在人类个体中评估对2型糖尿病的易感性的标记物的鉴定方法,该方法包括:
a.鉴定SEQ ID NO:1、SEQ ID NO:2或SEQ ID NO:3中的至少一个多态性标记物,或与其连锁不平衡的至少一个多态性标记物;
b.确定被诊断患有2型糖尿病或具有2型糖尿病易感性的个体的样品的基因型状态;以及
c.确定对照个体的样品的基因型状态;
其中在被诊断患有2型糖尿病或具有2型糖尿病易感性的个体中至少一个多态性的至少一个等位基因的频率,与对照样品中至少一个等位基因的频率相比的显著差异,指示了至少一个多态性可用于评估对2型糖尿病的易感性。
38.权利要求37的方法,其中连锁不平衡的特征为数值r2大于0.2和/或|D′|大于0.8。
39.权利要求37的方法,其中至少一个多态性标记物与选自下列标记物的至少一个标记物连锁不平衡,其中连锁不平衡的特征为数值r2大于0.2和/或|D′|大于0.8:rs2497304(SEQ ID NO:16)、rs947591(SEQ ID NO:30)、rs10882091(SEQ ID NO:4)、rs7914814(SEQ IDNO:24)、rs6583830(SEQ ID NO:20)、rs2421943(SEQ ID NO:15)、rs6583826(SEQ ID NO:19)、rs7752906(SEQ ID NO:32)、rs1569699(SEQ ID NO:6)、rs7756992(SEQ ID NO:21)、rs9350271(SEQ IDNO:33)、rs9356744(SEQ ID NO:34)、rs9368222(SEQ ID NO:35)、rs10440833(SEQ ID NO:36)、rs6931514(SEQ ID NO:37)、rs1860316(SEQ ID NO:10)、rs1981647(SEQ ID NO:11)、rs1843622(SEQ IDNO:9)、rs2191113(SEQ ID NO:13)、rs9890889(SEQ ID NO:31)。
40.权利要求37-39任一项的方法,其中在被诊断患有2型糖尿病或具有2型糖尿病易感性的个体中至少一个多态性的至少一个等位基因的频率,与对照样品中至少一个等位基因的频率相比的增加,指示了至少一个多态性可用于评估对2型糖尿病的增加的易感性。
41.权利要求37-39任一项的方法,其中在被诊断患有2型糖尿病或具有2型糖尿病易感性的个体中至少一个多态性的至少一个等位基因的频率,与对照样品中至少一个等位基因的频率相比的降低,指示了至少一个多态性可用于评估对2型糖尿病的降低的易感性,或针对2型糖尿病的保护。
42.对从人类个体获得的核酸样品进行基因分型的方法,包括确定样品中至少一个多态性标记物的至少一个等位基因的存在或不存在,其中至少一个标记物选自rs2497304(SEQ ID NO:16)、rs947591(SEQ ID NO:30)、rs10882091(SEQ ID NO:4)、rs7914814(SEQ IDNO:24)、rs6583830(SEQ ID NO:20)、rs2421943(SEQ ID NO:15)、rs6583826(SEQ ID NO:19)、rs7752906(SEQ ID NO:32)、rs1569699(SEQ ID NO:6)、rs7756992(SEQ ID NO:21)、rs9350271(SEQ IDNO:33)、rs9356744(SEQ ID NO:34)、rs9368222(SEQ ID NO:35)、rs10440833(SEQ ID NO:36)、rs6931514(SEQ ID NO:37)、rs1860316(SEQ ID NO:10)、rs1981647(SEQ ID NO:11)、rs1843622(SEQ IDNO:9)、rs2191113(SEQ ID NO:13)、rs9890889(SEQ ID NO:31),以及与其连锁不平衡的标记物,其中至少一个多态性标记物的至少一个等位基因的存在或不存在的确定,能预测对2型糖尿病的易感性。
43.权利要求41或42的方法,其中连锁不平衡由数值r2为至少0.2和/或数值|D′|为至少0.8来确定。
44.权利要求41-43任一项的方法,其中基因分型包括使用位于至少一个多态性标记物侧翼的核苷酸引物对,经聚合酶链反应(PCR)扩增含有该至少一个多态性标记物的核酸的区段。
45.权利要求41-44任一项的方法,其中基因分型使用选自等位基因特异性探针杂交、等位基因特异性引物延伸、等位基因特异性扩增、核酸测序、5’-外切核酸酶消化、分子信标分析、寡核苷酸连接分析、大小分析以及单链构象分析的方法来进行。
46.权利要求45的方法,其中方法包括等位基因特异性探针杂交。
47.权利要求45的方法,其中方法包括DNA测序。
48.权利要求45-47任一项的方法,包括:
1)将核酸的拷贝与检测寡核苷酸探针和增强子寡核苷酸探针在使寡核苷酸探针与核酸特异性杂交的条件下相接触;
其中
a)检测寡核苷酸探针长度为5-100个核苷酸,并与含有至少一个多态性位点、其核苷酸序列在SEQ ID NO:1、SEQ ID NO:2或SEQ ID NO:3中给出的核酸的第一个区段特异性杂交;
b)检测寡核苷酸探针在其3’末端含有可检测标记,在其5’末端含有淬灭基团;
c)增强子寡核苷酸长度为5-100个核苷酸,并与相对于寡核苷酸探针5’方向的核苷酸序列的第二个区段互补,以便当两个寡核苷酸都与核酸杂交时,增强子寡核苷酸位于检测寡核苷酸探针的3’方向;以及
d)在第一个区段和第二个区段之间存在单个碱基缺口,使得当寡核苷酸探针和增强子寡核苷酸探针都与核酸杂交时,在寡核苷酸之间存在单个碱基缺口;
2)使用当检测探针与核酸杂交时将从检测探针的3’末端裂解可检测标记以释放游离的可检测标记的内切核酸酶来处理核酸;以及
3)测量游离的可检测标记,其中游离的可检测标记的存在表明检测探针与核酸的第一个区段特异性杂交,并表明多态性位点的序列与检测探针互补。
49.权利要求48的方法,其中核酸的拷贝通过聚合酶链反应(PCR)扩增来提供。
50.权利要求37-49任一项的方法,其中易感性是增加的易感性。
51.权利要求37-49任一项的方法,其中易感性是降低的易感性。
52.权利要求37-50任一项的方法,其中个体具有特定的人类血统。
53.权利要求52的方法,其中血统选自非洲黑人血统、高加索人血统和中国人血统。
54.权利要求52或53的方法,其中血统是非洲黑人血统。
55.权利要求52的方法,其中血统是欧洲人血统。
56.权利要求52或53的方法,其中血统是高加索人血统。
57.权利要求52-56任一项的方法,其中血统是自己报告的。
58.权利要求52-56任一项的方法,其中血统是通过包括在个体的核酸样品中检测至少一个多态性标记物的至少一个等位基因的遗传测定来确定的,其中等位基因的存在或不存在是个体血统的指示。
59.权利要求37-58任一项的方法,其中个体是肥胖的。
60.权利要求37-58任一项的方法,其中个体是不肥胖的。
61.用于评估个体对预防和/或缓解与2型糖尿病相关的症状的治疗药剂的反应的可能性的方法,包括:在从个体获得的核酸样品中确定至少一个多态性标记物的至少一个等位基因的存在或不存在,其中至少一个多态性标记物选自rs2497304(SEQ ID NO:16)、rs947591(SEQID NO:30)、rs10882091(SEQ ID NO:4)、rs7914814(SEQ ID NO:24)、rs6583830(SEQ ID NO:20)、rs2421943(SEQ ID NO:15)、rs6583826(SEQ ID NO:19)、rs7752906(SEQ ID NO:32)、rs1569699(SEQ IDNO:6)、rs7756992(SEQ ID NO:21)、rs9350271(SEQ ID NO:33)、rs9356744(SEQ ID NO:34)、rs9368222(SEQ ID NO:35)、rs10440833(SEQ ID NO:36)、rs6931514(SEQ ID NO:37)、rs1860316(SEQ IDNO:10)、rs1981647(SEQ ID NO:11)、rs1843622(SEQ ID NO:9)、rs2191113(SEQ ID NO:13)、rs9890889(SEQ ID NO:31),以及与其连锁不平衡的标记物,其中至少一个标记物的至少一个等位基因的存在的确定,指示了对2型糖尿病治疗药剂的阳性反应的可能性。
62.权利要求61的方法,其中2型糖尿病治疗药剂选自药剂表1和药剂表2中显示的药剂。
63.预测被诊断患有2型糖尿病的个体的预后的方法,该方法包括确定从个体获得的核酸样品中至少一个多态性标记物的至少一个等位基因的存在或不存在,其中至少一个多态性标记物选自rs2497304(SEQ ID NO:16)、rs947591(SEQ ID NO:30)、rs10882091(SEQ IDNO:4)、rs7914814(SEQ ID NO:24)、rs6583830(SEQ ID NO:20)、rs2421943(SEQ ID NO:15)、rs6583826(SEQ ID NO:19)、rs7752906(SEQ ID NO:32)、rs1569699(SEQ ID NO:6)、rs7756992(SEQ IDNO:21)、rs9350271(SEQ ID NO:33)、rs9356744(SEQ ID NO:34)、rs9368222(SEQ ID NO:35)、rs10440833(SEQ ID NO:36)、rs6931514(SEQ ID NO:37)、rs1860316(SEQ ID NO:10)、rs1981647(SEQ IDNO:11)、rs1843622(SEQ ID NO:9)、rs2191113(SEQ ID NO:13)、rs9890889(SEQ ID NO:31),以及与其连锁不平衡的标记物,其中至少一个等位基因的存在的确定,指示了个体2型糖尿病的更糟的预后。
64.监测经历2型糖尿病治疗的个体的治疗进展的方法,该方法包括确定从个体获得的核酸样品中至少一个多态性标记物的至少一个等位基因的存在或不存在,其中至少一个多态性标记物选自rs2497304(SEQ ID NO:16)、rs947591(SEQ ID NO:30)、rs10882091(SEQ IDNO:4)、rs7914814(SEQ ID NO:24)、rs6583830(SEQ ID NO:20)、rs2421943(SEQ ID NO:15)、rs6583826(SEQ ID NO:19)、rs7752906(SEQ ID NO:32)、rs1569699(SEQ ID NO:6)、rs7756992(SEQ IDNO:21)、rs9350271(SEQ ID NO:33)、rs9356744(SEQ ID NO:34)、rs9368222(SEQ ID NO:35)、rs10440833(SEQ ID NO:36)、rs6931514(SEQ ID NO:37)、rs1860316(SEQ ID NO:10)、rs1981647(SEQ IDNO:11)、rs1843622(SEQ ID NO:9)、rs2191113(SEQ ID NO:13)、rs9890889(SEQ ID NO:31),以及与其连锁不平衡的标记物,其中至少一个等位基因的存在的确定,指示了个体的治疗结果。
65.权利要求61-64任一项的方法,其中连锁不平衡由数值r2为至少0.2和/或数值|D′|为至少0.8来确定。
66.前述权利要求任一项的方法,还包括评估个体的样品中的至少一种生物标记物。
67.前述权利要求任一项的方法,还包括分析个体的非遗传信息以进行风险评估、诊断或预后。
68.权利要求67的方法,其中非遗传信息选自年龄、性别、种族、社会经济状态、以前的疾病诊断、对象的医学史、2型糖尿病的家族史、生物化学测量和临床测量。
69.权利要求66-68任一项的方法,还包括计算总风险。
70.用于在人类个体中评估对2型糖尿病的易感性的试剂盒,该试剂盒含有选择性检测个体的基因组中至少一个多态性标记物的至少一个等位基因的存在或不存在的试剂,其中多态性标记物选自其序列显示在SEQ IN NO:1、SEQ IN NO:2和SEQ IN NO:3中的核酸区段中的多态性标记物,以及与其连锁不平衡的标记物,其中至少一个等位基因的存在指示了对2型糖尿病的易感性。
71.权利要求70的试剂盒,其中至少一个多态性标记物选自表10-12中显示的标记物,以及与其连锁不平衡的标记物。
72.权利要求70或71的试剂盒,其中至少一个多态性标记物选自rs2497304(SEQ ID NO:16)、rs947591(SEQ ID NO:30)、rs10882091(SEQ ID NO:4)、rs7914814(SEQ ID NO:24)、rs6583830(SEQ IDNO:20)、rs2421943(SEQ ID NO:15)、rs6583826(SEQ ID NO:19)、rs7752906(SEQ ID NO:32)、rs1569699(SEQ ID NO:6)、rs7756992(SEQ ID NO:21)、rs9350271(SEQ ID NO:33)、rs9356744(SEQ IDNO:34)、rs9368222(SEQ ID NO:35)、rs10440833(SEQ ID NO:36)、rs6931514(SEQ ID NO:37)、rs1860316(SEQ ID NO:10)、rs1981647(SEQ ID NO:11)、rs1843622(SEQ ID NO:9)、rs2191113(SEQ IDNO:13)、rs9890889(SEQ ID NO:31),以及与其连锁不平衡的标记物。
73.权利要求69-72任一项的试剂盒,其中连锁不平衡由数值r2为至少0.2和/或数值|D′|为至少0.8来确定。
74.权利要求70-73任一项的试剂盒,其中试剂含有至少一个与个体的基因组的含有至少一个多态性标记物的片段杂交的连续的寡核苷酸、缓冲液和可检测标记。
75.权利要求70-74任一项的试剂盒,其中试剂含有至少一对与从对象获得的基因组核酸区段的反向链杂交的寡核苷酸,其中每个寡核苷酸引物对被设计成能选择性扩增个体的基因组中包含一个多态性标记物的片段,其中片段的大小为至少30个碱基对。
76.权利要求74或75的试剂盒,其中至少一个寡核苷酸与个体的基因组完全互补。
77.权利要求74-76任一项的试剂盒,其中寡核苷酸的长度为大约18到大约50个核苷酸。
78.权利要求74-77任一项的试剂盒,其中寡核苷酸的长度为20-30个核苷酸。
79.权利要求70-78任一项的试剂盒,其中试剂盒含有:
a.长度为5-100个核苷酸的检测寡核苷酸探针;
b.长度为5-100个核苷酸的增强子寡核苷酸探针;以及
c.内切核酸酶;
其中检测寡核苷酸探针与其核苷酸序列在SEQ ID NO:1、SEQ IDNO:2或SEQ ID NO:3中给出核酸的含有至少一个多态性位点的第一个区段特异性杂交;并且
其中检测寡核苷酸探针在其3’末端含有可检测标记,在其5’末端含有淬灭基团;
其中增强子寡核苷酸长度为5-100个核苷酸,并与相对于寡核苷酸探针5’方向的核苷酸序列的第二个区段互补,以便当两个寡核苷酸都与核酸杂交时,增强子寡核苷酸位于检测寡核苷酸探针的3’方向;
其中在第一个区段和第二个区段之间存在单个碱基缺口,使得当寡核苷酸探针和增强子寡核苷酸探针都与核酸杂交时,在寡核苷酸之间存在单个碱基缺口;并且
其中当检测探针与核酸杂交时,使用内切核酸酶处理核酸将从检测探针的3’末端裂解可检测标记,以释放游离的可检测标记。
80.寡核苷酸探针在制造用于在人类个体中诊断和/或评估2型糖尿病易感性的诊断试剂中的应用,其中探针与其核苷酸序列在SEQ IDNO:1、SEQ ID NO:2或SEQ ID NO:3中给出核酸的含有至少一个多态性位点的区段杂交,其中片段的长度为15-500个核苷酸。
81.权利要求80的应用,其中多态性位点选自多态性标记物rs2497304(SEQ ID NO:16)、rs947591(SEQ ID NO:30)、rs10882091(SEQ ID NO:4)、rs7914814(SEQ ID NO:24)、rs6583830(SEQ IDNO:20)、rs2421943(SEQ ID NO:15)、rs6583826(SEQ ID NO:19)、rs7752906(SEQ ID NO:32)、rs1569699(SEQ ID NO:6)、rs7756992(SEQ ID NO:21)、rs9350271(SEQ ID NO:33)、rs9356744(SEQ IDNO:34)、rs9368222(SEQ ID NO:35)、rs10440833(SEQ ID NO:36)、rs6931514(SEQ ID NO:37)、rs1860316(SEQ ID NO:10)、rs1981647(SEQ ID NO:11)、rs1843622(SEQ ID NO:9)、rs2191113(SEQ IDNO:13)、rs9890889(SEQ ID NO:31),以及与其连锁不平衡的多态性。
82.计算机可读介质,其中储存有:
a.至少一个多态性标记物的识别符;
b.所述至少一个多态性标记物的至少一个等位基因在多个被诊断患有2型糖尿病的个体中的频率的指示符;以及
c.所述至少一个多态性标记物的至少一个等位基因在多个参比个体中的频率的指示符;
其中至少一个多态性标记物选自多态性标记物rs2497304(SEQ IDNO:16)、rs947591(SEQ ID NO:30)、rs10882091(SEQ ID NO:4)、rs7914814(SEQ ID NO:24)、rs6583830(SEQ ID NO:20)、rs2421943(SEQ ID NO:15)、rs6583826(SEQ ID NO:19)、rs7752906(SEQ IDNO:32)、rs1569699(SEQ ID NO:6)、rs7756992(SEQ ID NO:21)、rs9350271(SEQ ID NO:33)、rs9356744(SEQ ID NO:34)、rs9368222(SEQ ID NO:35)、rs10440833(SEQ ID NO:36)、rs6931514(SEQ IDNO:37)、rs1860316(SEQ ID NO:10)、rs1981647(SEQ ID NO:11)、rs1843622(SEQ ID NO:9)、rs2191113(SEQ ID NO:13)、rs9890889(SEQ ID NO:31),以及与其连锁不平衡的多态性,连锁不平衡被定义为数值r2为至少0.2和/或|D′|为至少0.8。
83.权利要求82的介质,还包含关于多个个体的血统的信息。
84.权利要求82或83的介质,其中被诊断患有2型糖尿病的多个个体和多个参比个体具有特定的血统。
85.用于在人类个体中测定2型糖尿病的遗传指示符的装置,包含:
a.计算机可读存储器:以及
b.储存在计算机可读存储器中的例行程序;
其中例行程序适用于在处理器上执行,以分析至少一个人类个体的至少一个多态性标记物的标记物和/或单倍型信息,其中所述至少一个多态性标记物选自标记物rs2497304(SEQ ID NO:16)、rs947591(SEQID NO:30)、rs10882091(SEQ ID NO:4)、rs7914814(SEQ ID NO:24)、rs6583830(SEQ ID NO:20)、rs2421943(SEQ ID NO:15)、rs6583826(SEQ ID NO:19)、rs7752906(SEQ ID NO:32)、rs1569699(SEQ IDNO:6)、rs7756992(SEQ ID NO:21)、rs9350271(SEQ ID NO:33)、rs9356744(SEQ ID NO:34)、rs9368222(SEQ ID NO:35)、rs10440833(SEQ ID NO:36)、rs6931514(SEQ ID NO:37)、rs1860316(SEQ IDNO:10)、rs1981647(SEQ ID NO:11)、rs1843622(SEQ ID NO:9)、rs2191113(SEQ ID NO:13)、rs9890889(SEQ ID NO:31),以及与其连锁不平衡的标记物,该连锁不平衡被定义为数值r2为至少0.2和/或|D′|的值为至少0.8,并根据标记物或单倍型信息产生输出,其中输出包括作为人类个体的2型糖尿病的遗传指示符的至少一个标记物或单倍型的风险测量。
86.权利要求85的装置,其中例行程序还包含多个被诊断患有2型糖尿病的个体中至少一个多态性标记物的至少一个等位基因或至少一个单倍型的频率的指示符,以及多个参比个体中至少一个多态性标记物的至少一个等位基因或至少一个单倍型的频率的指示符,其中风险测量是基于人类个体的至少一个标记物和/或单倍型状态与多个被诊断患有2型糖尿病的个体的至少一个标记物和/或单倍型信息的频率指示符的比较。
87.权利要求85或86的装置,其中风险测量由比值比(OR)或相对风险(RR)来表征。
88.在人类个体中测定对2型糖尿病的易感性的方法,包括测定在源自于个体的基因型数据组中是否存在至少一个多态性标记物的至少一个风险等位基因,其中至少一个多态性标记物选自表10-12中显示的标记物,以及与其连锁不平衡的标记物,其中至少一个风险等位基因的存在的确定,指示了个体对2型糖尿病的增加的易感性。
89.权利要求88的方法,其中至少一个多态性标记物显示在SEQID NO:1、SEQ ID NO:2或SEQ ID NO:3中。
90.权利要求88或89的方法,其中至少一个多态性标记物包括选自下列的至少一个标记物:rs2497304(SEQ ID NO:16)、rs947591(SEQ ID NO:30)、rs10882091(SEQ ID NO:4)、rs7914814(SEQ IDNO:24)、rs6583830(SEQ ID NO:20)、rs2421943(SEQ ID NO:15)、rs6583826(SEQ ID NO:19)、rs7752906(SEQ ID NO:32)、rs1569699(SEQ ID NO:6)、rs7756992(SEQ ID NO:21)、rs9350271(SEQ IDNO:33)、rs9356744(SEQ ID NO:34)、rs9368222(SEQ ID NO:35)、rs10440833(SEQ ID NO:36)、rs6931514(SEQ ID NO:37)、rs1860316(SEQ ID NO:10)、rs1981647(SEQ ID NO:11)、rs1843622(SEQ IDNO:9)、rs2191113(SEQ ID NO:13)、rs9890889(SEQ ID NO:31),以及与其连锁不平衡的标记物。
91.权利要求88-90任一项的方法,其中至少一个多态性标记物包括与选自表22、表23和表24中显示的标记物中的一个或多个标记物强烈连锁不平衡的至少一个标记物,强烈连锁不平衡被定义为数值|D′|大于0.8和/或r2大于0.2。
92.权利要求88-91任一项的方法,其中至少一个多态性标记物选自标记物rs7752906(SEQ ID NO:32)、rs1569699(SEQ ID NO:6)、rs7756992(SEQ ID NO:21)、rs9350271(SEQ ID NO:33)、rs9356744(SEQ ID NO:34)、rs9368222(SEQ ID NO:35)、rs10440833(SEQ IDNO:36)、rs6931514(SEQ ID NO:37),以及与其连锁不平衡的标记物。
93.权利要求92的方法,其中至少一个多态性标记物选自标记物rs7752906(SEQ ID NO:32)、rs1569699(SEQ ID NO:6)、rs7756992(SEQ ID NO:21)、rs9350271(SEQ ID NO:33)、rs9356744(SEQ IDNO:34)、rs9368222(SEQ ID NO:35)、rs10440833(SEQ ID NO:36)和rs6931514(SEQ ID NO:37)。
94.权利要求88的方法,其中至少一个多态性标记物选自标记物rs7756992(SEQ ID NO:21),以及与其连锁不平衡的标记物。
95.权利要求94的方法,其中至少一个多态性标记物选自表22中显示的标记物。
96.权利要求88的方法,其中至少一个标记物选自标记物rs10882091(SEQ ID NO:4)以及与其连锁不平衡的标记物。
97.权利要求96的方法,其中至少一个标记物选自表23中显示的标记物。
98.权利要求88的方法,其中至少一个标记物选自标记物rs2191113(SEQ ID NO:13)以及与其连锁不平衡的标记物。
99.权利要求98的方法,其中至少一个标记物选自表24中显示的标记物。
100.权利要求88-99任一项的方法,还包括评估个体中至少一种风险单倍型的频率,其中风险单倍型的存在的确定,指示了对2型糖尿病的增加的易感性。
101.权利要求88-100任一项的方法,其中增加的易感性的特征为相对风险(RR)或比值比(OR)为至少1.15。
102.权利要求88-101任一项的方法,其中增加的易感性的特征为相对风险(RR)或比值比(OR)为至少1.20。
103.权利要求88-102任一项的方法,其中rs2497304等位基因A、rs947591等位基因A、rs10882091等位基因C、rs7914814等位基因T、rs6583830等位基因A、rs2421943等位基因G、rs6583826等位基因G、rs7752906等位基因A、rs1569699等位基因C、rs7756992等位基因G、rs9350271等位基因A、rs9356744等位基因C、rs9368222等位基因A、rs10440833等位基因A、rs6931514等位基因G、rs1860316等位基因A、rs1981647等位基因C、rs1843622等位基因T、rs2191113等位基因A和/或rs9890889等位基因A的存在,指示了对2型糖尿病的增加的易感性。
104.权利要求88的方法,其中来自个体的基因型数据组中多态性标记物中风险等位基因的不存在的确定,指示了对2型糖尿病的降低的易感性。
105.权利要求88-104任一项的方法,其中连锁不平衡的特征为数值|D′|大于0.8和/或r2大于0.2。
106.权利要求88-105任一项的方法,还包括将对2型糖尿病的易感性表示为风险测量值。
107.权利要求106的方法,其中风险测量值被计算为相对风险(RR)或比值比(OR)。
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CN104388555A (zh) * | 2014-11-06 | 2015-03-04 | 中国科学院长春应用化学研究所 | Dna微阵列芯片检测与ⅱ型糖尿病相关的单核苷酸多态性的方法 |
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CN104388555A (zh) * | 2014-11-06 | 2015-03-04 | 中国科学院长春应用化学研究所 | Dna微阵列芯片检测与ⅱ型糖尿病相关的单核苷酸多态性的方法 |
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