CN101584377A - The method of producing animal fodder by fermenting brewery mash by composite thallus - Google Patents
The method of producing animal fodder by fermenting brewery mash by composite thallus Download PDFInfo
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- CN101584377A CN101584377A CNA2009101155545A CN200910115554A CN101584377A CN 101584377 A CN101584377 A CN 101584377A CN A2009101155545 A CNA2009101155545 A CN A2009101155545A CN 200910115554 A CN200910115554 A CN 200910115554A CN 101584377 A CN101584377 A CN 101584377A
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Abstract
A kind of method of producing animal fodder by fermenting brewery mash by composite thallus is characterized in that the preparation method is: 20 mesh sieves are dried, pulverize, crossed in (1) with brewex's grains; Or crushing fresh brewery mash; (2) regulate the solid-liquid ratio mixing with running water, wait to inoculate after the sterilization cooling; (3) fermented bacterium activates under all nutrition type culture medium condition, and enrichment culture 24h～30h prepares inoculation; (4) with sturdy crassa and lactic acid bacteria spread cultivation thalline under aseptic technique, volume ratio is inoculated in the actual production solid-state fermentation culture medium with 6%～8%, 8%～10% inoculum concentration by weight respectively; (5) brewex's grains that ferment are placed 70 ℃～80 ℃ dryings, promptly get the brewex's grains fermented feed.Advantage of the present invention is: 1. improve the brewex's grains quality, increase the utilization rate and the digestibility of feed; 2. microbial fermentation brewex's grains feedstuff and small investment of production equipment, benefit is big; 3. a large amount of grains are saved in minimizing environmental pollution, greatly reduce feed cost.
Description
Technical field
The present invention relates to a kind of method of producing animal fodder by fermenting brewery mash by composite thallus.
Background technology
The development of China brewing industry is swift and violent, and with about 6% speed increase, beer production is at the forefront in the world every year on average.Meanwhile brewex's grains output is also quite abundant, it is recycled significant.Abroad, the comprehensive utilization of brewex's grains is paid attention to very much, has been widely used in industry such as feed, medicine, food.China starts late, and these vinasse are never utilized preferably.Along with the shortage of feed resource and the enhancing of environmental consciousness, require feed industry to increase income and save grain to develop simultaneously the up to standard and dual consideration of turning waste into wealth of liquor-making enterprise blowdown.So brewex's grains become liquor-making enterprise and the common focus of paying close attention to of feed industry as feed in recent years.
Though the bright feed nutrient loss of feeding of brewex's grains is few; but its big wheat husk that contains has had a strong impact on trophic structure and digestive utilization ratio; and fresh beer vinasse is difficult to storage for a long time and fortune far away, and the consumption and the scope of application are limited, are difficult to adapt to scientific feeding, large-scale cultivation and beer and already develop rapidly.So utilize the microbial fermentation brewex's grains, producing animal feeding-stuff containing somatic protein is a potential direction of brewex's grains feed research, be characterized in utilizing microbial technique, carbohydrate in the raw material and inorganic nitrogen are changed into high protein feed, improve essential amino acids content, improve trophic structure, be beneficial to animal body and absorb better.Someone utilizes aspergillus niger, aspergillus oryzae, compound cooperative fermentation brewex's grains such as geotrichum candidum and candida utili are produced animal feed, are generally 25%～30% but the crude fibre degradation rate is low, crude protein raising rate is 4%～40%, is unfavorable for that so still animal digestion absorbs nutriment.Because the characteristic that brewex's grains complicated component and crude fibre are difficult to degrade, so screening is strong to brewex's grains crude fibre degradation capability, successful recombination energy improves the thalline of crude protein content of feed and grasps the technological parameter that they bring into play the best use of and seems particularly important.
Summary of the invention
The object of the present invention is to provide a kind of method of producing animal fodder by fermenting brewery mash by composite thallus, utilize the laboratory from row filter, strong sturdy crassa of bacterial strain of mutagenesis cellulase-producing ability and the compound synergy brewex's grains of laboratory preservation lactic acid bacteria are produced protein content and the higher novel fermentation feed of digestibility.
The present invention is achieved like this, and it is characterized in that the preparation method is:
(1) brewex's grains is dried, pulverizes, is crossed 20 mesh sieves; Account for 50%～70% by the quality brewex's grains, bean dregs or rice bran account for 20%～40%, (NH
4)
2SO
4Be the sturdy crassa fermenting and producing solid medium of 10% preparation, culture medium is through 90 ℃～100 ℃ dry method heat treated 15s～1min removal of impurities bacterium of going out, and the back is 1: 1 with running water adjusting solid-liquid ratio, and mixing is waited to inoculate; Or crushing fresh brewery mash, be 50%～70% by the quality brewex's grains, bean dregs or rice bran are 20%～40%, (NH
4)
2SO
410% through high-temperature steam sterilization 3min～10min, waits after the cooling to inoculate;
(2) fermented bacterium activation respectively under all nutrition type culture medium condition, enrichment culture is cultivated 24h～36h, is used for inoculation;
(3) sturdy crassa and lactic acid bacteria spread cultivation thalline under aseptic technique, volume ratio is inoculated in the actual production solid-state fermentation culture medium with 6%～8%, 8%～10% inoculum concentration by weight respectively, in environment temperature is 20 ℃～40 ℃, fermented and cultured 48h～120h;
(4) brewex's grains that ferment are placed 70 ℃～80 ℃ dryings, promptly get the brewex's grains fermented feed.
Of the present invention what be inoculated in the actual production solid-state fermentation culture medium is not the thalline spore, but has carried out the vegetative mycelium that enrichment spreads cultivation.
Advantage of the present invention is: 1. utilize sturdy crassa of mutagenesis and lactic acid bacteria composite fermentation brewex's grains can fully improve its crude protein content, reduce crude fibre significantly, increase content beta-carotene, improve the brewex's grains quality, increase the utilization rate and the digestibility of feed; 2. microbial fermentation brewex's grains feedstuff and small investment of production equipment, benefit is big; 3. utilize the microbial fermentation brewex's grains to turn waste into wealth, reduce environmental pollution, alleviate the pressure of protein feeds critical shortage, save a large amount of grains, greatly reduce feed cost.
The specific embodiment
Embodiment 1:
With fresh beer vinasse drying, the pulverization process that brewery produces, cross 20 mesh sieves, take by weighing the brewex's grains 7.0g of pulverizing, bean dregs 2.0g, (NH
4)
2SO
41.0g as the actual production culture medium, through 90 ℃～100 ℃ dry method heat treated 15s～1min, regulating solid-liquid ratio with running water is 1: 1.The thalline that will ferment simultaneously activates in slant medium, the enrichment of all nutrition type fluid nutrient medium.After sturdy crassa that enrichment is good and lactic acid bacteria insert actual production solid-state fermentation culture medium fermentation 60h～98h by 6% and 8% (v/w) inoculum concentration respectively, regulating temperature is 20 ℃～40 ℃, pH is a nature.Fermentation ends is drying to obtain the brewex's grains fermented feed.Measuring poor its crude fibre degradation rate of composite thallus fermentation beer is 40%, and the albumen growth rate is 34%, and beta carotene output is 8 μ g/g～15 μ g/g dry weight culture mediums.
Embodiment 2:
With the crushing fresh brewery mash that brewery produces, take by weighing the brewex's grains 70g of pulverizing, bean dregs 20g, (NH
4)
2SO
410g is as the actual production culture medium, and through high-temperature steam sterilization 3min～10min, the cooling back is to wait inoculate at 1: 1 with the running water adjusting solid-liquid ratio of pH 7.0.The thalline that will ferment simultaneously activates in slant medium, the enrichment of all nutrition type fluid nutrient medium.After sturdy crassa that enrichment is good and lactic acid bacteria insert actual production solid-state fermentation culture medium fermentation 100h～120h by 10% and 8% (v/w) inoculum concentration respectively, regulating temperature is 35 ℃～45 ℃, pH is a nature.Fermentation ends is drying to obtain the brewex's grains fermented feed.Measuring poor its crude fibre degradation rate of composite thallus fermentation beer is 37%, and the albumen growth rate is 38%, and beta carotene output is 10 μ g/g～18 μ g/g dry weight culture mediums.
Embodiment 3:
With the crushing fresh brewery mash that brewery produces, take by weighing the brewex's grains 6.8Kg of pulverizing, rice bran 1.7Kg, (NH
4)
2SO
41.0Kg, KH
2PO
40.5Kg as the actual production culture medium, through high-temperature steam sterilization 3min～10min, cooling back is to wait inoculate at 1: 1.5 with the running water adjusting solid-liquid ratio of pH7.0.The thalline that will ferment simultaneously activates in slant medium, the enrichment of all nutrition type fluid nutrient medium.After sturdy crassa that enrichment is good and lactic acid bacteria insert actual production solid-state fermentation culture medium fermentation 48h～60h by 8% and 10% (v/w) inoculum concentration respectively, regulating temperature is 40 ℃～50 ℃, pH is a nature.Fermentation ends is drying to obtain the brewex's grains fermented feed.Measuring poor its crude fibre degradation rate of composite thallus fermentation beer is 38.5%, and the albumen growth rate is 36%, and beta carotene output is 10 μ g/g～20 μ g/g dry weight culture mediums.
Claims (2)
1, a kind of method of producing animal fodder by fermenting brewery mash by composite thallus is characterized in that the preparation method is:
(1) brewex's grains is dried, pulverizes, is crossed 20 mesh sieves; Account for 50%～70% by the quality brewex's grains, bean dregs or rice bran account for 20%～40%, (NH
4)
2SO
4Be the sturdy crassa fermenting and producing solid medium of 10% preparation, culture medium is through 90 ℃～100 ℃ dry method heat treated 15s～1min removal of impurities bacterium of going out, and the back is 1: 1 with running water adjusting solid-liquid ratio, and mixing is waited to inoculate; Or crushing fresh brewery mash, be 50%～70% by the quality brewex's grains, bean dregs or rice bran are 20%～40%, (NH
4)
25O
410% through high-temperature steam sterilization 3min～10min, waits after the cooling to inoculate;
(2) fermented bacterium activation respectively under all nutrition type culture medium condition, enrichment culture is cultivated 24h～36h, is used for inoculation;
(3) sturdy crassa and lactic acid bacteria spread cultivation thalline under aseptic technique, volume ratio is inoculated in the actual production solid-state fermentation culture medium with 6%～8%, 8%～10% inoculum concentration by weight respectively, in environment temperature is 20 ℃～40 ℃, fermented and cultured 48h～120h;
(4) brewex's grains that ferment are placed 70 ℃～80 ℃ dryings, promptly get the brewex's grains fermented feed.
2, the method for producing animal fodder by fermenting brewery mash by composite thallus according to claim 1 is characterized in that described what be inoculated in the actual production solid-state fermentation culture medium is not the thalline spore, but has carried out the vegetative mycelium that enrichment spreads cultivation.
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| CNA2009101155545A CN101584377A (en) | 2009-06-19 | 2009-06-19 | The method of producing animal fodder by fermenting brewery mash by composite thallus |
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| CNA2009101155545A CN101584377A (en) | 2009-06-19 | 2009-06-19 | The method of producing animal fodder by fermenting brewery mash by composite thallus |
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102283315A (en) * | 2011-08-18 | 2011-12-21 | 南昌大学 | Method for producing animal probiotics feed by utilizing compound bacteria-fermented high fiber agricultural and sideline products |
| CN102286594A (en) * | 2011-08-18 | 2011-12-21 | 南昌大学 | Method for producing carotenoids by fermenting high-fiber subsidiary agricultural products |
| CN102356882A (en) * | 2011-10-19 | 2012-02-22 | 海南椰国食品有限公司 | Method utilizing brewer's grains to produce biological diet cellulose and product thereof |
| CN103156054A (en) * | 2011-12-08 | 2013-06-19 | 洛阳牧园自动控制设备有限公司 | Process for preparing liquid fermentation feed from fermented concentration solution |
| CN103156053A (en) * | 2011-12-08 | 2013-06-19 | 洛阳牧园自动控制设备有限公司 | Process for preparing liquid fermentation feed from distiller's grains |
| CN103404713A (en) * | 2013-06-24 | 2013-11-27 | 金为强 | Feed formula for meat ducks and preparation method of feed |
| CN104171265A (en) * | 2014-07-09 | 2014-12-03 | 四川农业大学 | Processing technology of single-cell protein from distilled spirit lees |
| CN104244729A (en) * | 2012-02-22 | 2014-12-24 | 波伊特研究股份有限公司 | Corn meal compositions and methods of production |
| CN105166395A (en) * | 2015-10-30 | 2015-12-23 | 何寒 | Moringa oleifera feed and preparing method thereof |
| CN106343151A (en) * | 2016-08-18 | 2017-01-25 | 深圳市中雁生物工程有限公司 | Production method of animal feed enriched in carotenoid and probiotics |
| CN109957514A (en) * | 2019-01-25 | 2019-07-02 | 南昌大学 | A kind of new application of sturdy crassa |
-
2009
- 2009-06-19 CN CNA2009101155545A patent/CN101584377A/en active Pending
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102283315A (en) * | 2011-08-18 | 2011-12-21 | 南昌大学 | Method for producing animal probiotics feed by utilizing compound bacteria-fermented high fiber agricultural and sideline products |
| CN102286594A (en) * | 2011-08-18 | 2011-12-21 | 南昌大学 | Method for producing carotenoids by fermenting high-fiber subsidiary agricultural products |
| CN102356882A (en) * | 2011-10-19 | 2012-02-22 | 海南椰国食品有限公司 | Method utilizing brewer's grains to produce biological diet cellulose and product thereof |
| CN102356882B (en) * | 2011-10-19 | 2012-10-31 | 海南椰国食品有限公司 | Method utilizing brewer's grains to produce biological diet cellulose and product thereof |
| CN103156054A (en) * | 2011-12-08 | 2013-06-19 | 洛阳牧园自动控制设备有限公司 | Process for preparing liquid fermentation feed from fermented concentration solution |
| CN103156053A (en) * | 2011-12-08 | 2013-06-19 | 洛阳牧园自动控制设备有限公司 | Process for preparing liquid fermentation feed from distiller's grains |
| CN104244729A (en) * | 2012-02-22 | 2014-12-24 | 波伊特研究股份有限公司 | Corn meal compositions and methods of production |
| CN103404713A (en) * | 2013-06-24 | 2013-11-27 | 金为强 | Feed formula for meat ducks and preparation method of feed |
| CN104171265A (en) * | 2014-07-09 | 2014-12-03 | 四川农业大学 | Processing technology of single-cell protein from distilled spirit lees |
| CN105166395A (en) * | 2015-10-30 | 2015-12-23 | 何寒 | Moringa oleifera feed and preparing method thereof |
| CN106343151A (en) * | 2016-08-18 | 2017-01-25 | 深圳市中雁生物工程有限公司 | Production method of animal feed enriched in carotenoid and probiotics |
| CN109957514A (en) * | 2019-01-25 | 2019-07-02 | 南昌大学 | A kind of new application of sturdy crassa |
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Application publication date: 20091125 |