CN102356882B - Method utilizing brewer's grains to produce biological diet cellulose and product thereof - Google Patents
Method utilizing brewer's grains to produce biological diet cellulose and product thereof Download PDFInfo
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- CN102356882B CN102356882B CN2011103179607A CN201110317960A CN102356882B CN 102356882 B CN102356882 B CN 102356882B CN 2011103179607 A CN2011103179607 A CN 2011103179607A CN 201110317960 A CN201110317960 A CN 201110317960A CN 102356882 B CN102356882 B CN 102356882B
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Abstract
The invention relates to a method utilizing brewer's grains to produce biological diet cellulose. The method comprises the steps of after adding water to the brewer's grains and diluting, adding or not adding other nutrient substances, and using the brewer's grains serving as a biological diet cellulose producing bacterium culture medium after sterilization; or adding water to the brewer's grains and jordaning, preserving heat for 1 to 2 hours at the temperature ranging from 60 DEG C to 90 DEG C, filtering and removing solid substances, adding or not adding other nutrient substances to filtrate, and using the filtrate as a culture medium after sterilization; or adding water to the brewer's grains and jordaning, adjusting pH to be 5 to 6, standing for 20 to 40 minutes, adding 5 wt% to 10 wt% of protease to filtrate after filtering and removing solid substances, performing enzymolysis for 1 to 3 hours while preserving heat at the temperature ranging from 50 DEG C to 70 DEG C, adjusting pH to be neutral, adding or not adding other nutrient substances after filtering and removing solid substances again, and using the filtrate serving as a culture medium after sterilization. The method can fully utilize nutrient components including crude protein, cellulose and the like in the brewer's grains so as to achieve good fermentation effect.
Description
Technical field
The present invention relates to the comprehensive reutilization technology of brewing industry accessory substance-brewex's grains, utilize method that brewex's grains produce biological dietary cellulosic and the biological dietary cellulosic product that obtains with this method in particular to a kind of.
Background technology
China's beer production just occupies first place, the world from beginning in 2002, and present annual production is about about 4,000 ten thousand tons.Brewex's grains are the main accessory substances in the Beer Production, and 1 ton of beer of every production will produce 1/4 ton brewex's grains.Brewex's grains are that process saccharification, the gelatinization technologies such as raw material Fructus Hordei Germinatus, rice and hops of producing beer is filtered or press filtration residual solid matter afterwards.Wherein contain a large amount of nutritional labelings, but owing to its moisture is bigger, rot easily, therefore most of producers use as feed to sell to the peasant at a low price, and have even direct discharging causes the waste of resource in the time of contaminated environment.Analysis is learnt, mainly contains crude protein and crude fibre in the brewex's grains dry, and wherein the content of crude protein surpasses 25%, and crude fibre also reaches about 13%, contains a spot of fat in addition, each seed amino acid, vitamin and mineral matter etc.
Dietary fiber is the dietary nutrient that a kind of difficult quilt digests, and it mainly comprises cellulose, hemicellulose, resin, pectin and lignin etc.Dietary fiber is keeping playing the part of important role on the digestion health, and absorbing enough dietary fibers simultaneously also can angiocardiopathy preventing, cancer, diabetes etc.Dietary fiber can clean wall of digestive, harmful substance or quicken it and excrete in the dilution food, and dietary fiber also can slow down the speed of digestion and accelerate the drainage of human body to cholesterol simultaneously, helps controlling blood sugar and cholesterol.
Biology cellulose (Biocellulose) is meant under different condition; By synthetic cellulosic general designation such as certain microorganism in acetic acid Pseudomonas (Acetobacter), Agrobacterium (Agrobacterium), rhizobium (Rhizobium) and the Sarcina (Sarcina) etc., be called bacteria cellulose again.Biology cellulose has very high water retention property, can keep high-moisture for a long time, and has very high biofacies content and high biodegradability etc.It is the good dietary fiber source of rising at present.At present; Produce biology cellulose and typically use coconut water, also have the various carbohydrates of use and beef extract, peptone etc. directly to be configured to liquid culture medium, still as fermentation medium; The source of coconut water is limited by region, and uses the culture medium cost of configuration high.In the production of biology cellulose, do not use the report of brewex's grains at present.
Summary of the invention
To above-mentioned defective; The present invention provides a kind of method of utilizing brewex's grains to produce biological dietary cellulosic; Comprise in the step: behind the brewex's grains thin up; Sterilization directly produces bacterium culture medium as biological dietary cellulosic and uses, or adds other nutriment behind the brewex's grains thin up, sterilizes and uses as biological dietary cellulosic generation bacterium culture medium.
Wherein amount of water does not have special restriction, still is preferably 1-3 times of brewex's grains weight.
The present invention also provides a kind of method of utilizing brewex's grains to produce biological dietary cellulosic, comprises in the step: brewex's grains are added the water mill slurry; Be incubated 1-2 hour down at 60-90 ℃ then; Remove by filter solid matter, directly produce bacterium culture medium after the filtrating sterilization and use, or filtrating adds to sterilize behind other nutriment again and produces bacterium culture medium as biological dietary cellulosic and use as biological dietary cellulosic.
The 1-3 that the addition that wherein adds water mill when slurry water is preferably brewex's grains weight doubly.
The present invention also provides a kind of method of utilizing brewex's grains to produce biological dietary cellulosic, comprises in the step: brewex's grains are added the water mill slurry, transfer pH to 5-6, left standstill 20-40 minute; The protease that adds 5-10 weight % behind the solids removed by filtration material again in the filtrating; Be incubated 50-70 ℃ enzymolysis 1-3 hour; Adjust pH is to neutral; Filter to remove behind the solid matter sterilization once more and directly produce bacterium culture medium and use, or to add behind other nutriment that sterilization remakes be that biological dietary cellulosic produces bacterium culture medium and uses as biological dietary cellulosic.
Wherein, the 1-3 that the addition that adds water mill when slurry water is preferably brewex's grains weight doubly; Protease wherein is preferably and is selected from pepsin, trypsase, one or more in the alkali protease.
Other nutriment described in above-mentioned 3 kinds of methods is selected from coconut water, sucrose, glucose, one or more in beef extract, the yeast extract.
Can also comprise in above-mentioned 3 kinds of methods: produce bacterium, the step of fermentation to produce biological dietary cellulosic film toward the biological dietary cellulosic of the inoculation of medium of processing.In addition, can also comprise the step biological dietary cellulosic film that obtains is dry, that pulverize.
The present invention also provides a kind of biological dietary cellulosic product, and it is to obtain through above-mentioned arbitrary method.
Adopt method of the present invention, make full use of the nutritional labeling in the accessory substance-brewex's grains of Beer Production, reduce and pollute, energy savings.Employing adds that water mill slurry, insulation leave standstill, the processing step of filter and remove residue, can make the nutriment stripping in the brewex's grains, has also removed impurity such as cot wherein, leaf bud, thereby has obtained better ferment effect; And adopt basic hydrolysis can increase the solubility property of the insoluble fibrin that contains in the brewex's grains itself, improve the mouthfeel of dietary fiber, the output of raising dietary fiber; Protease hydrolytic can be peptide or amino acid with macromolecular proteolysis, improves bioavilability, promotes fermentation, can obtain better ferment effect.Promptly containing dietary cellulosic that biofermentation makes in the product that adopts the present invention to obtain and contain the dietary cellulosic that contains in the brewex's grains itself and other vitamin, mineral matter and other components again, is the good source that the human diet cellulose replenishes.
The specific embodiment
Below in conjunction with embodiment, specific embodiments of the invention describes in further detail.Following examples are used to explain the present invention, but are not used for limiting scope of the present invention.
Embodiment 1:
After getting the water dilution of weight such as brewex's grains add; Directly produce bacterium culture medium through high-temperature sterilization as biological dietary cellulosic; The gluconate pyracetobacillus seed liquor of inoculation medium 10 volume %, 30 ℃ of bottom fermentations 7 days obtain biological dietary cellulosic film in media surface.
Embodiment 2:
Get the water mill slurry that brewex's grains add 2 times of its weight; Be incubated 2 hours down at 60 ℃ then; Remove by filter solid matter; High-temperature sterilization produces bacterium culture medium as biological dietary cellulosic after the coconut water of weight such as adding in the filtrating; The gluconate pyracetobacillus seed liquor of inoculation medium 8 volume %, 28 ℃ of bottom fermentations 5 days obtain biological dietary cellulosic film in media surface.
Embodiment 3:
Get the water mill slurry that brewex's grains add 3 times of its weight; Be incubated 1 hour down at 90 ℃ then; Remove by filter solid matter; High-temperature sterilization produces bacterium culture medium as biological dietary cellulosic after adding beef extract and the 1 weight % sodium dihydrogen phosphate of sucrose, 3 weight % of 10 weight % in the filtrating; The gluconate pyracetobacillus seed liquor of inoculation medium 9 volume %; 32 ℃ of bottom fermentations 6 days obtain biological dietary cellulosic film in media surface.Results should biology dietary cellulosic film, and heated-air drying is to constant weight, pulverize dietary cellulosic powder product.
Embodiment 4:
Get the water mill slurry that brewex's grains add 3 times of its weight, transfer pH to 5, left standstill 40 minutes; The protease that adds 5 weight % behind the solids removed by filtration material again in the filtrating; Be incubated 70 ℃ of enzymolysis 1 hour; Adjust pH is to neutral, and high-temperature sterilization produced bacterium culture medium, the gluconate pyracetobacillus seed liquor of inoculation medium 10 volume % as biological dietary cellulosic after solid matter was removed in filtration once more; 30 ℃ of bottom fermentations 6 days obtain biological dietary cellulosic film in media surface.Results should biology dietary cellulosic film, and heated-air drying is to constant weight, pulverize dietary cellulosic powder product.
Embodiment 5:
Get the water mill slurry that brewex's grains add 2 times of its weight, transfer pH to 5.5, left standstill 30 minutes; The protease that adds 8 weight % behind the solids removed by filtration material again in the filtrating; Be incubated 60 ℃ of enzymolysis 2 hours, adjust pH is to neutral, after solid matter is removed in filtration once more; High-temperature sterilization produces bacterium culture medium as biological dietary cellulosic after adding the coconut water of 1.5 times of filtrating weight in the filtrating; Inoculate the gluconate pyracetobacillus seed liquor of culture medium 12 volume %, 30 ℃ of bottom fermentations 5 days obtain biological dietary cellulosic film in media surface.
Embodiment 6:
Get the water mill slurry of weight such as brewex's grains adding, transfer pH to 6, left standstill 20 minutes; The protease that adds 10 weight % behind the solids removed by filtration material again in the filtrating; Be incubated 50 ℃ of enzymolysis 3 hours; Adjust pH is to neutral, and high-temperature sterilization produced bacterium culture medium, the gluconate pyracetobacillus seed liquor of inoculation medium 9 volume % as biological dietary cellulosic after solid matter was removed in filtration once more; 32 ℃ of bottom fermentations 7 days obtain biological dietary cellulosic film in media surface.Results should biology dietary cellulosic film, and heated-air drying is to constant weight, pulverize dietary cellulosic powder product.
Promptly containing the biology cellulose that biofermentation makes in the product that obtains in the foregoing description, contain the dietary cellulosic that contains in the brewex's grains itself and other vitamin, mineral matter and other components again, is the good source that the human diet cellulose replenishes.Can process directly that dietary supplements is daily takes or process other various forms of food products,, enlarge biology cellulose food applications market like jelly, sour milk, beverage or the like.
The above only is a preferred implementation of the present invention; Should be pointed out that for those skilled in the art, under the prerequisite that does not break away from know-why of the present invention; Can also make some improvement and retouching, these improvement and retouching also should be regarded as protection scope of the present invention.
Claims (5)
1. method of utilizing brewex's grains to produce biological dietary cellulosic, it is characterized in that: step comprises: brewex's grains are added the water mill slurry, transfer pH to 5-6; Left standstill 20-40 minute; The protease that adds 5-10 weight % behind the solids removed by filtration material again in the filtrating, be incubated 50-70 ℃ enzymolysis 1-3 hour, adjust pH is to neutrality; Sterilization produces the bacterium culture medium use as biological dietary cellulosic after filtering the removal solid matter once more; Or sterilization remakes to biological dietary cellulosic produces the bacterium culture medium use after adding other nutriment, inoculates biological dietary cellulosic and produces bacterium, fermentation to produce biological dietary cellulosic film; Wherein said protease is one or more in pepsin, trypsase, the alkali protease.
2. the method for utilizing brewex's grains to produce biological dietary cellulosic according to claim 1 is characterized in that: the addition that brewex's grains add water mill when slurry water be brewex's grains weight 1-3 doubly.
3. the method for utilizing brewex's grains to produce biological dietary cellulosic according to claim 1 is characterized in that: described other nutriment is selected from coconut water, sucrose, glucose, one or more in beef extract, the yeast extract.
4. the method for utilizing brewex's grains to produce biological dietary cellulosic according to claim 1 is characterized in that: also comprise in the step: the step that the biological dietary cellulosic film that will obtain is dry, step that pulverize is processed biological dietary cellulosic powder product.
5. the biological dietary cellulosic product that obtains through arbitrary method among the claim 1-4.
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| CN2011103179607A CN102356882B (en) | 2011-10-19 | 2011-10-19 | Method utilizing brewer's grains to produce biological diet cellulose and product thereof |
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| CN2011103179607A CN102356882B (en) | 2011-10-19 | 2011-10-19 | Method utilizing brewer's grains to produce biological diet cellulose and product thereof |
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| CN102356882A CN102356882A (en) | 2012-02-22 |
| CN102356882B true CN102356882B (en) | 2012-10-31 |
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Families Citing this family (4)
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| CN103773824B (en) * | 2014-01-08 | 2015-12-02 | 陕西科技大学 | A kind of fermentation medium for bacterial cellulose and utilize this substratum to produce the method for bacteria cellulose |
| CN108149506B (en) * | 2018-02-03 | 2020-03-10 | 贵州华清科维环境能源有限责任公司 | Method for producing high-purity cellulose, hemicellulose and lignin by using vinasse |
| CN111254698B (en) * | 2020-02-25 | 2020-10-09 | 山东科技大学 | Process for preparing superfine functional fiber from vinasse |
| CN112280103B (en) * | 2020-10-29 | 2022-03-15 | 巢湖学院 | Antibacterial packaging film and preparation method thereof |
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| KR100530996B1 (en) * | 2003-08-27 | 2005-11-28 | 경북대학교 산학협력단 | Method for Producing Bacterial Cellulose Using the Waste of Beer Fermentation Broth |
| CN1765226A (en) * | 2005-11-21 | 2006-05-03 | 中国食品发酵工业研究院 | Method for producing diet fiber, feedstuff oil and protein powder simultaneously by use of wheat grains |
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| CN101584377A (en) * | 2009-06-19 | 2009-11-25 | 南昌大学 | The method of producing animal fodder by fermenting brewery mash by composite thallus |
| CN101869130A (en) * | 2010-06-10 | 2010-10-27 | 山东农业大学 | High-protein dietary fiber enriched bread and making method thereof |
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| KR100530996B1 (en) * | 2003-08-27 | 2005-11-28 | 경북대학교 산학협력단 | Method for Producing Bacterial Cellulose Using the Waste of Beer Fermentation Broth |
| CN1765226A (en) * | 2005-11-21 | 2006-05-03 | 中国食品发酵工业研究院 | Method for producing diet fiber, feedstuff oil and protein powder simultaneously by use of wheat grains |
| CN1966423A (en) * | 2006-08-01 | 2007-05-23 | 广东海大实业有限公司 | Beer lees waste water processing method |
| CN101584377A (en) * | 2009-06-19 | 2009-11-25 | 南昌大学 | The method of producing animal fodder by fermenting brewery mash by composite thallus |
| CN101869130A (en) * | 2010-06-10 | 2010-10-27 | 山东农业大学 | High-protein dietary fiber enriched bread and making method thereof |
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