CN101574396A - Compound preparation of fructus schisandrae and evening primrose CO* supercritical extract and preparation process - Google Patents

Abstract

The invention relates to a compound preparation of fructus schisandrae and evening primrose CO2 supercritical extract and a preparation process. The process is characterized by: grinding fruit of fructus schisandrae and seeds of evening primrose respectively to 40-60 meshes and putting the ground substances in a supercritical extraction device for extraction with the pressure being 35MPa, the temperature being 35-40 DEG C, and the speed being 2L/min; and subsequently obtaining the fructus schisandrae and evening primrose CO2 supercritical extract by extracting 2.5-3h; and according to parts by weight, putting 50-52 of the fructus schisandrae CO2 supercritical extract, 47-49 of the evening primrose CO2 supercritical extract and 1.0 of vitamin E in a mixture tank to be stirred, mixed evenly and delivered to a soft capsule packing machine for pelleting. The compound preparation is advanced in dosage form, guarantees lasting efficacy of effective ingredients, is low in the content of impurities and high in the content of the effective ingredients with active ingredients easily being absorbed, and is safer for eating and is a natural health food with CO2 used as extraction dissolvent and no residue of organic solvent.

Description

A kind of Radix Schisandrae Bicoloris and Radix Oenotherae erythrosepalae CO 2Supercritical extract compound formulation and preparation technology

Technical field:

The present invention discloses a kind of Radix Schisandrae Bicoloris and Radix Oenotherae erythrosepalae CO ₂The supercritical extract compound formulation is a kind of food with protection islet cells, auxiliary hyperglycemic function, the invention also discloses the preparation technology of above-mentioned compound formulation, belongs to health product technology field.

Background technology:

Fructus Schisandrae Chinensis is the fruit of magnoliaceae schisandra (FructusSchisandraeChinensis).Scientific research shows that Fructus Schisandrae Chinensis is different because of the place of production, north and south difference effect.The Fructus Schisandrae Sphenantherae color is red, can treat the cough due to wind and cold, and the Radix Schisandrae Bicoloris darkish complexion can be treated the body sweating due to debility and be hindered diseases such as essence more.That has now developed has Fructus Schisandrae Chinensis natural juice, Fructus Schisandrae Chinensis fruit juice, Fructus Schisandrae Chinensis fruit jelly, a Fructus Schisandrae Chinensis fruit wine etc., and common dosage forms medicinal or health product are syrup, electuary.Because the main functional component of Fructus Schisandrae Chinensis is the lignanoids material, mainly the method with decocting in water obtains, the extracts active ingredients of this extracting method is very incomplete, particularly the functional component in kernel and the shell can not fully be extracted, throw away with medicinal residues mostly, waste valuable drug resource, increased production cost of products.

The Radix Oenotherae erythrosepalae liposoluble constituent be applicable to have some setbacks menstrual period, climacteric discomfort, rheumatic arthritis and multiple degenerative joint, improve allergic constitution, the complication of pant, releive allergic eczema and antipruritic, confirmed fatigue, prevention fatty liver and liver cirrhosis, prevent diabetes, eliminate fat, cholesterol reducing, prevention of cardiac, preventing thrombosis, prevention of stroke etc. in advance.

Do not see the report of Fructus Schisandrae Chinensis and Radix Oenotherae erythrosepalae compound formulation by retrieval.

Summary of the invention:

The invention provides a kind of Radix Schisandrae Bicoloris and Radix Oenotherae erythrosepalae CO ₂The supercritical extract compound formulation utilizes both synergism to protect islet cells and auxiliary blood sugar lowering.

Radix Schisandrae Bicoloris and Radix Oenotherae erythrosepalae CO have been the present invention further provides ₂The preparation technology of supercritical extract compound formulation is suitable for suitability for industrialized production.

Radix Schisandrae Bicoloris of the present invention and Radix Oenotherae erythrosepalae CO ₂The supercritical extract compound formulation is characterized in that being made up of following raw material by weight:

Fructus Schisandrae Chinensis CO ₂Supercritical extract: 50～52

Radix Oenotherae erythrosepalae CO ₂Supercritical extract: 47～49

Vitamin E: 1.0

The preparation of described compound formulation may further comprise the steps:

Fruit of Fructus Schisandrae Chinensis is crushed to 40 orders ~ 60 orders, puts into CO ₂In the supercritical extraction unit, with 35MPa pressure; 40 ℃ of extraction temperature; 2L/min; Extract and obtained Fructus Schisandrae Chinensis CO in 3 hours ₂Supercritical extract;

Evening primrose seed is crushed to 40 orders ~ 60 orders, puts into CO ₂In the supercritical extraction unit, with 35MPa pressure; 35 ℃ of extraction temperature, 35 ℃ of separation temperatures; 2L/min; Extract and obtained Radix Oenotherae erythrosepalae CO in 2.5 hours ₂Supercritical extract;

With Fructus Schisandrae Chinensis CO ₂Supercritical extract, Radix Oenotherae erythrosepalae CO ₂Supercritical extract and vitamin E are put into material-compound tank in proportion and are mixed, and send into soft capsule packer pelleting.

Be animal model with the genotype diabetes model below, prove the therapeutic effect of compound formulation of the present invention type ii diabetes.

1 material

1.1 animal

Genotype diabetes KK-A ^yMice (male, body weight 25g ± 5g); The C57BL/6J mice (male, body weight 25g ± 5g), and all available from Chinese Academy of Medical Sciences's animal center, the quality certification: SYXK (army) 2002-042.

1.2 medicine

Metformin hydrochloride: Hebei Tiancheng Pharmaceutical Co., Ltd (former Cangzhou pharmaceutical factory);

Compound formulation of the present invention is provided by this laboratory.

2 experimental techniques

2.1 laboratory animal grouping and administration

KK-A ^yMice high glucose and high fat forage feed, the C57BL/6J mice feeds and gets final product with normal diet.KK-A ^yThe mice adaptability is raised and was surveyed blood glucose in the 3rd day, the 7th day, and the blood glucose value of twice mensuration basicly stable (fasting is 12 hours before surveying) is got the KK-A of blood glucose value greater than 11.1mol/L as a result ^yMice is included experiment in.And with KK-A ^yMice is divided into model group, high dose group (200mg/kg/d), middle dosage group (100mg/kg/d), low dose group (25mg/kg/d) and positive controls (metformin hydrochloride) (200mg/kg/d) at random by blood glucose value, and normal group and model group give the normal saline of equivalent 0.9%.Gastric infusion, successive administration take off neck and put to death after 35 days.

2.2 the mensuration of blood glucose value

Minute: diabetes heredity KK-A ^yMice administration 35 days, during measure week about and respectively organize the mice fasting blood sugar.

Oral glucose tolerance test (OGTT): the phase is carried out an oral glucose tolerance test in test.Before the experiment, each organized the mice fasting 12 hours, surveyed blood glucose, as the 0min blood glucose value, irritated stomach then and gave respectively to organize mice G/W (2g/kg), timing, the blood glucose value when tail vein is got blood survey 30min, 60min, 120min.

The tail vein is got the mensuration of blood and blood glucose: survey the preceding fasting of blood glucose 12 hours, can't help water.The Mus tail exposes the tail venous engorgement with cotton ball soaked in alcohol wiping sterilization, cuts osculum with knife blade on vein, and outflow 1 is bled and dropped on the blood sugar test paper measurement window, writes down reading behind the 5s.When repeatedly measuring, otch is shifted to near-end from far-end.

3 results

5.2.2.1 the invention medicine is to diabetes KK-A ^yThe influence of mouse blood sugar

Model group and normal group mouse blood sugar value keep more stable level; Along with the increase of administration natural law, administration group blood glucose value all becomes downward trend.Wherein, invention medicine high dose and middle dosage treatment group be the blood sugar lowering level obviously, and be remarkable with the model group comparing difference, has statistical significance (P＜0.01); But the blood glucose value level of low dose therapy group and model group comparing difference are not remarkable, not statistically significant (P＞0.05).Metformin hydrochloride group blood glucose value obviously descends, with remarkable (P＜0.001) (the seeing Table 1) of model group comparing difference.

Table 1 invention medicine is to diabetes KK-A ^yThe influence of mouse blood sugar (n=10, x ± s)

Annotate: compare * P＜0.05, * * P＜0.01 with model group

5.2.2.2 oral glucose tolerance test (OGTT) result

After irritating stomach G/W 120min, invention medicine high dose group blood glucose value has obvious decline, and relatively there were significant differences with model group, has statistical significance (P＜0.01); Dosage group and low dose group blood glucose value also decrease in the invention medicine, and be more variant with model group, has statistical significance (P＜0.05); Three dosage groups of invention medicine with dosage be increased in 120min the time blood glucose value corresponding on a declining curve.Metformin hydrochloride group blood glucose value blood glucose value when 120min has obvious decline, and relatively there were significant differences with model group, has statistical significance (P＜0.01).Behind the injectable dextrose monohydrate, each administration group day part blood glucose value all is lower than model group (seeing Table 2).

Table 2 oral glucose tolerance experimental result (n=10, x ± s)

Annotate: compare * P＜0.05, * * P＜0.01 with model group

" clinical researches of Radix Oenotherae erythrosepalae oil breast treatment diabetes " (" Chinese endocrine metabolism magazine " // 1991 (7) 2:106-107) according to Jin Chaojun etc. show that Radix Oenotherae erythrosepalae oil breast blood sugar decreasing effect is: blood glucose value 10.4 ± 2.78 before the treatment, treatment back blood glucose value 7.68 ± 2.74 only reduces by 2.82.

Compound formulation of the present invention can reduce for blood glucose value and reaches 4.28.This is because Radix Oenotherae erythrosepalae blood sugar lowering and the synergistic result of Fructus Schisandrae oil protection islet cells.

Adopt soft capsule by totally enclosed form, content and air are completely cut off, avoid the destruction of light simultaneously, prevent the oxidation of content effective ingredient and lose effectiveness or contaminated, enhanced stability photoactive substance.Because the effective ingredient of soft capsule goods exists with liquid condition, dissolubility is good, has guaranteed your the high absorption rate to effective ingredient.

Adopt CO ₂The supercritical extraction unit pressurization also contains a large amount of lignan components with containing a large amount of lipid components in extract of effective ingredient, particularly Fructus Schisandrae Chinensis kernel in the shell of Fructus Schisandrae Chinensis kind Renhe more in the shell, reduced the wasting of resources.CO ₂Be extraction solvent extract at low temperature, guarantee that the thermal sensitivity functional component is not destroyed, organic solvent-free is residual, and is edible safer.

Good effect of the present invention is: prescription is based on pure Chinese medicinal preparation, utilizes the synergy curative effect type ii diabetes of Radix Oenotherae erythrosepalae oil and Fructus Schisandrae oil, and treating both the principal and secondary aspects of a disease is evident in efficacy, has no adverse reaction, and has no side effect.

The specific embodiment:

The present invention will be further described below in conjunction with embodiment:

Embodiment 1:

Fruit of Fructus Schisandrae Chinensis is crushed to 40 orders, puts into CO ₂In the supercritical extraction unit, with 35MPa pressure; 40 ℃ of extraction temperature; 2L/min; Extract and obtained Fructus Schisandrae Chinensis CO in 3 hours ₂Supercritical extract.

Evening primrose seed is crushed to 40 orders, puts into CO ₂In the supercritical extraction unit, with 35MPa pressure; 35 ℃ of extraction temperature, 35 ℃ of separation temperatures; 2L/min; Extract and obtained Radix Oenotherae erythrosepalae CO in 2.5 hours ₂Supercritical extract.

With Fructus Schisandrae Chinensis CO ₂Supercritical extract is 52kg, evening primrose seed CO ₂Supercritical extract is that 47kg and vitamin E are that 1.0kg puts into material-compound tank and mixes, and sends into soft capsule packer pelleting.

Embodiment 2:

Fruit of Fructus Schisandrae Chinensis is crushed to 60 orders, puts into CO ₂In the supercritical extraction unit, with 35MPa pressure; 40 ℃ of extraction temperature; 2L/min; Extract and obtained Fructus Schisandrae Chinensis CO in 3 hours ₂Supercritical extract.

Evening primrose seed is crushed to 60 orders, puts into CO ₂In the supercritical extraction unit, with 35MPa pressure; 35 ℃ of extraction temperature, 35 ℃ of separation temperatures; 2L/min; Extract and obtained Radix Oenotherae erythrosepalae CO in 2.5 hours ₂Supercritical extract.

With Fructus Schisandrae Chinensis CO ₂Supercritical extract is 51kg, evening primrose seed CO ₂Supercritical extract is that 48kg and vitamin E are that 1.0kg puts into material-compound tank and mixes, and sends into soft capsule packer pelleting.

Embodiment 3:

Fruit of Fructus Schisandrae Chinensis is crushed to 50 orders, puts into CO ₂In the supercritical extraction unit, with 35MPa pressure; 40 ℃ of extraction temperature; 2L/min; Extract and obtained Fructus Schisandrae Chinensis CO in 3 hours ₂Supercritical extract.

Evening primrose seed is crushed to 50 orders, puts into CO ₂In the supercritical extraction unit, with 35MPa pressure; 35 ℃ of extraction temperature, 35 ℃ of separation temperatures; 2L/min; Extract and obtained Radix Oenotherae erythrosepalae CO in 2.5 hours ₂Supercritical extract.

With Fructus Schisandrae Chinensis CO ₂Supercritical extract is 50kg, evening primrose seed CO ₂Supercritical extract is that 49kg and vitamin E are that 1.0kg puts into material-compound tank and mixes, and sends into soft capsule packer pelleting.

Claims (2)

1, a kind of Radix Schisandrae Bicoloris and Radix Oenotherae erythrosepalae CO ₂The supercritical extract compound formulation is characterized in that being made up of following raw material by weight:

Fructus Schisandrae Chinensis CO ₂Supercritical extract: 50～52

Radix Oenotherae erythrosepalae CO ₂Supercritical extract: 47～49

Vitamin E: 1.0.

2, the described preparation of soft capsule technology of claim 1 may further comprise the steps:

Fruit of Fructus Schisandrae Chinensis is crushed to 40 orders-60 order, puts into CO ₂In the supercritical extraction unit, with 35MPa pressure; 40 ℃ of extraction temperature; 2L/min; Extract and obtained Fructus Schisandrae Chinensis CO in 3 hours ₂Supercritical extract;

Evening primrose seed is crushed to 40 orders-60 order, puts into CO ₂In the supercritical extraction unit, with 35MPa pressure; 35 ℃ of extraction temperature, 35 ℃ of separation temperatures; 2L/min; Extract and obtained Radix Oenotherae erythrosepalae CO in 2.5 hours ₂Supercritical extract;

With Fructus Schisandrae Chinensis CO ₂Supercritical extract, Radix Oenotherae erythrosepalae CO ₂Supercritical extract and vitamin E are put into material-compound tank in proportion and are mixed, and send into soft capsule packer pelleting.