RU2114628C1 - Component composition "adaptovit" showing antihypoxic effect - Google Patents

Abstract

FIELD: medicine. SUBSTANCE: composition has the following components, %: aralia roots 8-12; schizandra seeds 8-12; nettle leaves 13-17; mountain ash fruits 3-7; viburnum fruits 3-7; wild rose fruits 3-7, and Eleutherococcus senticosus roots - the rest. Composition can be used for organism resistance increase to oxygen decreased content (to enhance resistance to hypoxia), prophylaxis of intoxication with agents inducing formation of methemoglobin and as tonic agent. Preparation can be used also for prophylaxis and correction of functional disorders and sicknesses caused by effect of stress and environment factors, different radiation forms and so on. EFFECT: enhanced effectiveness of composition. 2 cl

Description

 The present invention relates to medicine, namely to the creation of herbal remedies, which are further used as tonic and restorative agents, to increase the body's resistance to low oxygen content (increase resistance to hypoxia), to prevent poisoning with hemoglobin formers, as well as for prevention and correction functional disorders and diseases associated with the impact on the body of stress and exhausting environmental factors.

 Known medicinal plants with adaptogenic activity, such as eleutherococcus, aralia, lemongrass [1, 4].

 Eleutherococcus and aralia are known to have a good adaptogenic effect against the background of vitamin saturation [2, 3, 8].

 Nettle is known to be used as a multivitamin, tonic and hemoglobin-enhancing agent [1,9].

 Rosehip, viburnum and mountain ash are known as sources of trace elements and multivitamins with a general strengthening effect [1, 9, 10].

 Known tool with tonic and restorative effect "Energoton", which is closest to the problem being solved. It consists of concentrated grape juice, apple and black chokeberry extracts, water-alcohol extracts from rosehip, hawthorn, aralia and eleutherococcus [5].

 The present invention was the development of a new drug based on available domestic raw materials with specific antihypoxic activity.

This goal is achieved by the fact that the proposed natural composition containing the roots of Eleutherococcus, aralia roots, magnolia vine seeds, nettle leaves, rowan fruits, viburnum fruits, rose hips in the following ratio, wt.%:
Aralia roots - 8-12
Schisandra seeds - 8 - 12
Nettle Leaves - 13 - 17
Rowan fruits - 3 - 7
Fruits of viburnum - 3 - 7
Rose hips - 3 - 7
Eleutherococcus Roots - Else
The developed natural composition has a pronounced adaptogenic, tonic, stimulating, general strengthening effect, and also has a specific specific antihypoxic activity, which will make it possible to use it as a potential means to increase the body's resistance to low oxygen content (increase resistance to hypoxia), and to prevent poisoning by hemoglobin-forming agents.

 The listed plants were included in the composition on the basis of a known spectrum of their pharmacological properties, and the collection components and their parts were selected so that antihypoxant properties were potentiated.

Firstly, all three adaptogens - Eleutherococcus, Aralia and Schisandra - have different chemical compositions. The main active ingredients of the roots of Eleutherococcus are eleutherosides - derivatives of various groups of natural compounds, mainly phenolic in nature. The action of the roots of aralia is mainly due to triterpene glycosides-aralosides. The pharmacological effect of lemongrass seeds is determined mainly by lignans (schizandrins, schisandrol, etc.)
Various biologically active complexes have a pharmacological effect that differs in mechanisms, and determine the specific activity of each of these adaptogens. The use of a universal extractant allowed to increase the biologically active complex, which led to an increase in the pharmacological effect.

 Secondly, in the proposed composition, tonic objects are combined with vitamin ones.

 Nettle is used as a multivitamin, tonic and hemoglobin increasing agent. Rosehip, viburnum and mountain ash are used as sources of trace elements and multivitamins with a general strengthening effect. These objects are also characterized by a high content of carotenoids, phenolic compounds, in particular flavonoids, anthocyanins, catechins, which provides antioxidant properties of the proposed composition.

 The proposed dosage form of the drug provides the extraction of biologically active substances of different polarity: in addition to the groups that are traditionally attributed the adaptogenic and tonic effect (aralosides, eleutherosides, schizandrin), chlorophylls, carotenoids, vitamins C and K, flavonoids pass into the extract.

 Collection method: take the roots of Eleutherococcus (50 g), aralia roots (10 g), magnolia vine seeds (10 g), nettle leaves (15 g), rowan fruits (5 g), viburnum fruits (5 g), rose hips (5 g ) All collection components are crushed to the size of particles passing through a 3-4 mm sieve.

 The alcohol extract is prepared as follows: the above mixture of plant components is extracted with 40% ethyl alcohol at a ratio of 1: 1 (raw material: solvent) by percolation.

 The specific antihypoxic activity of Adaptovit is due to the result of the synergistic action of its constituent components.

 Pharmacological studies were performed on animals.

 The experiments were conducted on white outbred male mice weighing 18-20 g. Animals from the Stolbovaya RAMS nursery were kept under standard vivarium conditions.

 An aliquot of the complex plant extract was evaporated on a rotary evaporator (water bath) until ethyl alcohol was completely removed. The residue was dissolved in saline 1: 10 and 1: 100 and used in the experiment. Animals were injected with 0.1 - 0.25 ml of these solutions per 10 g of weight. The indicated doses were selected in preliminary experiments, taking into account the need to obtain the effects of the drug, corresponding to the introduction of its average therapeutic and large doses.

 To assess the effect of the studied complex plant extract on the resistance of mice to hypoxic effects, a model of hemic hypoxia with the introduction of sodium nitrate was chosen [6].

 Acute hemic (circulatory) hypoxia (OHG) in mice was induced by injecting a solution of sodium nitrate at a dose of 300 mg / kg 1 hour after intraperitoneal administration of the studied drugs under the skin of the back. With the introduction of sodium nitrate in the indicated dose, the life span of the control animals is 18–25 min at 100% mortality (LD 100 300 mg / kg), which corresponds to the Methodological Recommendations of the Pharmacological Committee of the Ministry of Health of Russia [6].

 An analysis of the results of the experiments allows us to conclude that a single administration of a complex plant extract in doses of 10 mg / kg (solution 1:10) and especially 25 mg / kg (solution 1: 10) has a pronounced antihypoxic effect with OHG, comparable with the effect of officinal antidote-methylene blue, antihypoxant - tomerzole and significantly exceeds the antihypoxic effects of ascorbic acid and actoprotector - bemitil.

 At the same time, another ability of the complex plant extract was discovered, which forces one to pay attention to this drug as a potential tool for the prevention of poisoning with methemoglobin formers - the ability to prevent the death of experimental animals (mice) at doses of nitric oxide derivatives exceeding LD 100.

 An analysis of the results of the experiments also allows us to conclude that a single administration of a complex plant extract in doses of 10 mg / kg (solution 1: 10) and especially 25 mg / kg (solution 1: 10) has a pronounced antihypoxic effect in acute hypobaric hypoxia (OGBG), comparable with the effect of the antihypoxant tomerzole and exceeding the antihypoxic effects of the nootropic piracetam, the psychostimulant sydnocarb and the drug for comparison of ranntarin.

 The LD 50 of the complex plant extract was determined by the method of V. Prozorovsky et al. [7]. The studied extract was administered intraperitoneally, once.

 The experiments used white outbred male mice weighing 28-32 g.

 An analysis of the experimental results led to the conclusion that the daily toxicity of the complex plant extract is 25.7 mg / kg (20.0-33.0).

 Based on the established pharmacological activity of the collection in combination with a low level of toxicity and known data on adaptogenic, restorative properties included in the collection of components, Adaptovit can be recommended for widespread use as an adaptogenic, tonic and restorative agent to increase the body's resistance to low levels oxygen, for the prevention of poisoning with methemoglobin formers, as well as for the prevention and correction of functional disorders and diseases associated with the impact on the body of stressful and exhausting environmental factors, various forms of radiation.

Sources of information
1. Sokolov S. Ya., Zamrtaev I.P. Handbook of medicinal plants. -M .: Medicine, 1984. p. 464.

 2. Ivanchenko V.A., Grodzinsky A.M., Cherevchenko T.M. et al. Phytoergonomics. Edited by Academician of the Ukrainian Academy of Sciences of Grodzinsky A.M. Kiev: N. Dumka, 1989. p. 296.

 Z. Tsyrenzhapova O.D. Principles of pharmacological correction of adaptive processes Tez. Dokl. conf. "Medicinal plants in traditional and folk medicine." - Ulan-Ude, 1983. p. 33-35.

 4. Eleutherococcus and other adaptogens from Far Eastern medicinal plants (Materials for the study of ginseng and other medicinal products of the Far East, issue 7) .- Vladivostok: edition of the Far East Branch of the USSR Academy of Sciences, 1966.

 5. Patent N 2018 316 A 61 K 35/78. Publ. Bull. N 16 08/30/94.

 6. Guidelines for the study of antihypoxic properties of compounds in the experiment. M.-Pharmacological Committee of the Ministry of Health of the USSR.-1990.

 7. Prozorovsky V. B., Prozorovskaya M. N., Demchenko V. M. Express method for determining the average effective dose and its errors. (Pharmacol. And toxicol. - 1978. - N 4. - p. 497-502.

 8. Meersen F.Z. Adaptation, stress and prevention. - M., 1981, - p. 278.

 9. Kashinsky V. G. Plants in the treatment and prevention of diseases. -Tomsk, 1989 .-- p. 289.

 10. Lovkova M.Ya., Rabinovich A.M. Ponomareva S.M. and others. Why plants are treated.-M .: Nauka, 1990. p. 256.

Claims (1)

1. The composition of ingredients with antihypoxic effect, containing eleutherococcus roots, aralia roots, rowan fruits, rose hips, characterized in that it additionally contains lemongrass seeds, viburnum fruits, nettle leaves in the following ratio of ingredients, wt.%:
Aralia roots - 8 - 12
Schisandra seeds - 8 - 12
Nettle Leaves - 13 - 17
Rowan fruits - 3 - 7
Fruits of viburnum - 3 -7
Rose hips - 3 - 7
Eleutherococcus Roots - Else
2. The composition according to claim 1, characterized in that it is an alcoholic extract.