KR20020031911A - crude drug for 2type diabetes - Google Patents

Abstract

PURPOSE: A crude drug formulation containing an extract of Ginseng radix rubra, Acanthopanax Senticosus and Cordyceps sinensis as a main component is provided which has significantly increased efficacy in treating a human suffering from the Syndrome of Type 2 Diabetes. CONSTITUTION: This crude drug formulation contains 60.4% by weight of a Ginseng radix rubra extract, 23.1% by weight of an Acanthopanax Senticosus extract, 4.6% by weight of a Cordyceps sinensis extract and multi-vitamins(0.046% by weight of B1, 0.046% by weight of B2, 0.046% by weight of B6, 0.002% by weight of B12, 2.3% by weight of C and 0.46% by weight of E).

Description

Crude drug for 2type diabetes

The present invention relates to a herbal drug that is effective for type 2 diabetes, and more particularly, to an herbal drug that is effective for treating type 2 diabetes, which is mainly composed of extracts of red ginseng, barberry, cordyceps and multi-vitamin.

Prickly Ogapi used in the present invention means one of the plants included in the genus Ogapi, to avoid the duplication of the following examples and the like only representatively refers to prickly Ogapi species.

In the present invention, the extracts of the leaves, stems or roots of red ginseng, spiny gingival and Cordyceps sinensis and multi-vitamin were used as the main ingredients, and the mixing ratios of the extracts of the red ginseng, spiny ginseng and Cordyceps sinensis were tested for 1 month in KKA ^Y mice, a type 2 diabetic experimental animal. As a result of oral administration of fasting blood glucose levels, blood glucose levels were markedly reduced, leading to the invention of herbal preparations to treat type 2 diabetes.

The KKA ^Y mouse refers to a laboratory mouse genetically manipulated to type 2 diabetes.

Diabetes is a disease in which unburned sugars are excreted in the urine and medically diagnosed as diabetes when the blood sugar level is high. Normal blood glucose levels in healthy people are 80-120 mg per 1 deciliter of blood. If hyperglycemia above this level persists, blood circulation worsens and cell damage is incurred. Insufficient or not produced at all is called type 1 diabetes, and when insulin is properly secreted, it is inactivated and not used to convert sugar into energy. Type 2 diabetes is known. Patient.

In other words, when insulin regulates the blood glucose level in the body, if the action of insulin is normal, even though the absorbed sugar is somewhat higher, the blood glucose level can be maintained by promoting the use of the body, but in the state where the insulin is not active, the absorption of the sugar in the cell is normal. Because it is not made, the sugar in the blood will increase.

In order to treat this type of diabetes, a diet controlled by food intake and exercise exercises with regular and moderate exercise may promote insulin secretion, or may take medicine or inject insulin to allow the insulin to function normally. It was a common treatment.

Until now, diabetic experiments have been performed by injecting chemicals (STZ, alloxan, etc.) into the experimental animals used to damage pancreatic beta cells, which are largely dependent on the dose to which the chemicals are administered. It was a model that had been destroyed much of the close to the type 1 diabetes model with little insulin secretion ability, and also a considerable distance from human diabetes. Therefore, a significant error was expected to search for the efficacy of the samples using this disease model.

The present invention is to provide a new type 2 antidiabetic herbal drug with a clear hypoglycemic effect by supplementing the problems of the hypoglycemic experiment so far and ultimately improving insulin resistance.

The present invention relates to a herbal preparation that is effective in type 2 diabetes, and prepared by varying the ratio of the extracts of red ginseng, spiny ginseng, leaf, stem or root of Cordyceps sinensis and multi-vitamins, and the red ginseng, spiny ginseng, Cordyceps sinensis extract. It is a herbal medicine.

Red ginseng ( Ginseng Radix rubra ) is a dried dried bark of Panax ginseng , which acts mainly on the lungs and the nasal air. Anti-aging, liver function and hangover elimination effect, anti-radiation effect, energetic enhancement, anti-tumor effect, and anti-diabetic effect of hyperglycemia by alloxan administration.

Gasiohgapi tree (A. Senticosus) is called to the herbal which mainly acts on the liver (肝) and the new (腎) of the plant of ohgagwa (Araliaceae), such as ginseng,五加皮the muscular fatigue and bark of Acanthopanax hawthorn plants (Acanthopanacis cortex) Increasing immune function and bio-resistance, enhancing energy and learning ability, preserving liver function and detoxification, restraint stress gastric ulcer, inhibiting histamine release, anti-radiation, improving myocardial infarction, and hyperglycemia by administration of alloxan Saponin fraction of leaf extract shows hypoglycemic effect.

Cordyceps sinensis, along with ginseng and antler, has been treated as a precious three herbal medicines, and has been used as an antidote for tuberculosis, jaundice and opioid poisoning.

As mentioned above, red ginseng, thorn stem, and Cordyceps sinensis are already known for their inherent effects, and even though antidiabetic activity has been reported, alloxan or STZ-induced hyperglycemia was close to the type 1 diabetes model. However, the experiment of the present invention confirmed that the herbal medicine of the present invention is effective in treating type 2 diabetes, which is the form of most diabetic patients.

In the present invention, a conventional medicinal herb extractor was used to obtain the selected medicinal herb extract.

Although 10-100% ethanol or distilled water was selected as the extraction solvent, the selection of the extraction solvent is not important. Therefore, distilled water extraction solvent is representatively described in the following examples.

In the medicine extraction process, the same conditions apply to all selected medicine ingredients.

The experiment was repeated by applying an extraction temperature of 30 to 100 ℃ and an extraction time of 1-10 hours. Herein, the change of extraction temperature and extraction time is an experimental condition that can be easily changed by those skilled in the art.

In the case of administering the extract of the present invention for clinical purposes, it is preferable to separate the dose once or two to three times a day and the total daily dose to be administered to the host is in the range of 1 to 50 mg per kg of body weight, but is specific for a specific patient. Dosage levels may vary depending on the specific compound to be used, weight, age, sex, health condition, diet, time of administration, method of administration, rate of excretion, drug mixture and severity of the disease.

The compounds of the present invention can be administered as injections or oral formulations as desired.

Injectable preparations, for example sterile injectable aqueous or oleaginous suspensions, can be prepared using suitable dispersing agents, wetting agents or suspending agents according to known techniques. At this time, solvents that may be used include water, Ringer's solution and physiological saline solution, and sterile fixed oils are commonly used as a solvent or suspending medium. Any non-irritating fixed oil may be used for this purpose, including mono- and diglycerides, and fatty acids such as oleic acid may also be used in the preparation of injectables.

Dosage forms for oral administration may be capsules, tablets, pills, powders, granules, and solutions. Particularly, capsules, tablets, and solutions are useful. Tablets and pills are preferably prepared with enteric agents. Solid and liquid dosage forms can be used to prepare the herbal extracts according to the present invention with one or more inert diluents such as sucrose, lactose, starch, etc. It is prepared by mixing with a carrier selected from preservatives such as ate, sweeteners such as sugar or fructose and surfactants. More specifically, a capsule may be prepared by adding starch and lactose as an excipient in addition to the active ingredient in a ratio of 7: 3 and adding magnesium stearate and talc to within 3% to increase fluidity; Tablets can be prepared by adding starch and lactose as an excipient in a ratio of 7: 3 and adding calcium binder with a binder and a disintegration aid and tableting; A liquid preparation can be prepared by adding fruit flavor as a fragrance, sodium benzoate as a preservative, sugar or fructose as a sweetener and a surfactant.

The present invention may change the experimental conditions in the extraction process of extracting the selected medicinal herbs, the general manufacturing process conditions do not affect the invention given to the selection of the medicinal extracts of the present invention.

Therefore, the operating conditions in the extraction process and final herbal preparation manufacturing process specified in the following examples are only representative of the numerical values, and such numerical specification and limitation do not affect the composition of the present invention.

Example

Example 1

① First step

Preparation of Red Ginseng Extract

Red ginseng was repeatedly extracted twice with distilled water at 100 ° C. for 2 hours, concentrated under reduced pressure and lyophilized to obtain red ginseng extract.

② Second step

Preparation of Prickly Pear Extract

Prickly stems were repeatedly extracted twice at 100 ° C. for 2 hours, concentrated under reduced pressure and lyophilized to obtain thorny stem extracts.

③ Third step

Preparation of Cordyceps Sinensis Extract

Cordyceps sinensis was repeatedly extracted twice at 100 ° C. for 2 hours, concentrated under reduced pressure, and lyophilized to obtain a cordyceps extract.

④ 4th step

Preparation of herbal multi-vitamin blends

20 to 80% by weight of the red ginseng extract obtained in the first step, 80 to 20% by weight of the bark extract extracted in the second step, and 1 to 5% by weight of the Cordyceps sinensis obtained in the third step, were mixed [Vitamin (B ₁ 0.046% by weight) , B ₂ 0.046 wt%, B ₆ 0.046 wt%, B ₁₂ 0.002 wt%, C 2.3 wt%, E 0.46 wt%) multi-vitamin.

In the fourth step, the mixing ratio may be 69.4% by weight of red ginseng extract, 23.1% by weight of thorny oocyte extract, and 4-6% by weight of Cordyceps sinensis.

In the above embodiment can be applied to select the leaves or roots in place of the selected stems.

In the present invention, which part of the prickly pear extract is collected is not an important factor that determines the effect of the present invention. Thus, thorny thorns extract refers to extracts on the leaves, stems or roots of thorny thorns, based on the facts identified from repeated experiments involving thorny thorns.

The present invention can be prepared by a number of methods to extract the extract of the thorny scabies or Cordyceps sinensis, which are represented by the red ginseng extract and thorny bark bark in order to obtain the anti-type 2 diabetes effect as described in the above examples. However, the extraction method presented above is not limited to solvent extraction after mixing each herbal medicine.

In addition, the presence state of the final extract may be in any form of liquid or powder.

Selected medicinal herbs and their composition ratio for hyperglycemic lowering due to type 2 diabetes, which is an obvious effect of the present invention, 20 to 80% by weight of red ginseng extract, 80 to 20% by weight of prickly pear extract

, Cordyceps sinensis extract 1 to 5% by weight, most preferably red ginseng extract 60 to 70% by weight

, 20-30% by weight of thorny thorns extract, 1-5% by weight of Cordyceps sinensis extract.

Experimental Example 1

Type 2 diabetes experiment

As a type 2 diabetes model, KKA ^Y mice were compared with fasting blood glucose before and after oral administration of red ginseng, spiny ginseng, Cordyceps sinensis and mixed extracts, or multi-vitamin mixed extracts. (Measurement of blood glucose level after oral administration on August 30, 2000) The inhibitory effect of the sample solution on the blood glucose increase was determined in each sample group for the difference in blood glucose levels before oral administration and after oral administration of the control group according to Equation 1 below. The inhibition rate of each sample group was calculated | required from ratio, and it evaluated.

The results are shown in Table 1.

Table 1. Glucose and Glucose Inhibition Rate after 14-Hour Fasting

division Control A B C D E Rosiglitazone Measurement date Blood glucose level (mg / dl) 90.0 ± 6.0 88.0 ± 15.9 78.6 ± 6.3 94.6 ± 20.4 108.5 ± 9.7 105.3 ± 7.5 86.5 ± 7.0 August 30 122.0 ± 28.0 119.3 ± 22.9 94.5 ± 3.1 105.0 ± 23.3 117.0 ± 25.5 113.1 ± 10.2 92.7 ± 10.0 September 26 Blood sugar suppression rate (%) - 2.2 50.3 ^* 67.5 73.5 ^* 75.6 ^* 80.6 ^* -

A: red ginseng (300 mg / kg)

B: visiogapi (300 mg / kg)

C: Cordyceps sinensis (300 mg / kg)

D: Red ginseng (300 mg / kg) + thorn ogapi (100 mg / kg) + Cordyceps sinensis (20 mg / kg)

E: Red ginseng (300 mg / kg) + thorny ogapi (100 mg / kg) + Cordyceps sinensis (20 mg / kg) + Vitamin (B ₁ 0.2mg / kg, B ₂ 0.2mg / kg, B ₆ 0.2mg / kg, B ₁₂ 0.2 μg / kg, C 10 mg / kg, E 2 mg / kg)

The numerical value shown in Table 1 above is an average value obtained by six experiments, and " ^* " means that P <0.05.

As shown in Table 1. Results of 1 month oral administration of KKA ^Y hyperglycemic rats, type 2 diabetes model, for each extract, extract extract and extract extract of red ginseng, prickly pear, Cordyceps sinensis Fasting blood glucose showed a significant inhibitory effect of 50.3% of the extracts of red ginseng, barberry, and Cordyceps sinensis, but the extracts of red ginseng, barberry, and Cordyceps were higher than 75.6% and 73.5%. It showed strong blood sugar rise inhibition rate. These results were confirmed to show a synergistic effect, unlike the case of single administration by mixing red ginseng, prickly pear, cordyceps and Vitamine. Rosiglitazone, a positive control drug, also showed a strong inhibitory effect on blood glucose elevation of 80.6%.

Claims (4)

Herbal medicine that is effective in type 2 diabetes, consisting of red ginseng, prickly pear, Cordyceps sinensis extract. According to claim 1, 20 to 80% by weight of red ginseng extract, 80 to 20% by weight of prickly pear extract, and 1 to 5% by weight of Cordyceps sinensis, a herbal preparation effective for type 2 diabetes. The herbal formulation according to claim 1, which is effective for type 2 diabetes, mixed with multi-vitamins. According to claim 3, 69.4% by weight of the red ginseng extract, 23.1% by weight of the bark extract, 4.6% by weight of Cordyceps sinensis and multi-vitamin [Vitamin (B ₁ 0.046% by weight, B ₂ 0.046% by weight, B ₆ 0.046% by weight, B ₁₂ 0.002% by weight, C 2.3% by weight, consisting of 0.46% by weight] E, herbal drugs which are effective in type 2 diabetes.