CN101550393B - Reactor for microalgae outdoor breeding - Google Patents

Reactor for microalgae outdoor breeding Download PDF

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Publication number
CN101550393B
CN101550393B CN 200910106956 CN200910106956A CN101550393B CN 101550393 B CN101550393 B CN 101550393B CN 200910106956 CN200910106956 CN 200910106956 CN 200910106956 A CN200910106956 A CN 200910106956A CN 101550393 B CN101550393 B CN 101550393B
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reactor
algae liquid
microalgae
unit
reactor unit
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CN101550393A (en
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王兆凯
呼延子龙
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Shenzhen Jawkai Bioengineering R&D Center Co Ltd
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Shenzhen Jawkai Bioengineering R&D Center Co Ltd
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02Photobioreactors
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/02Form or structure of the vessel
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/02Form or structure of the vessel
    • C12M23/18Open ponds; Greenhouse type or underground installations
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/18Flow directing inserts

Abstract

The invention discloses a reactor for microalgae outdoor breeding, including a reactor unit (1) located outdoor, a microalgae harvesting system (4) and an algal liquid circumfluence tube (22), etc., wherein, the reactor unit (1) is in ladder-like structure, is connected by a pluratliy of step-by-step reduced water-drop force eliminating unit (11) whose upstream side is convex with overflow weir (12) from upstream to downstream; when the algae body flowing from the upstream to the downstream meets the required concentration, the microalgae is harvested by the microalgae harvesting system (4), when it does not meet the required concentration, the algal liquid is transferred and returned to the upstream of the reactor unit (1) through the algal liquid circumfluence tube (22). The inventive reactor uses ladder-type flow to generate onflow, so that the distribution of the algae body in the algal liquid is more uniform, the invention has more higher carbon dioxide utilization, low-cost, easymaintenance and repair, the specific algal species and methods can not easily be polluted by imported microalgae, and is suitable for large-scale, low-cost, continuous or semi-continuous breeding of microalgae.

Description

The reactor drum that is used for microalgae outdoor breeding
Technical field
The present invention relates to a kind of equipment of both culturing microalgae, relate in particular to a kind of reactor drum that is used for the little algae of outdoor breeding, be adapted at outdoor extensive, low-cost cultivating microalgae.
Background technology
Traditional both culturing microalgae reactor drum mainly contains two types: closed reactor and open reactive device.Closed reactor mainly contains several types such as tubular reactor, two vertical plates formula reactor drum, bag reactor and stirring tank formula, and closed reactor is difficult for polluting in breeding process, and frond concentration is high in the water body; The both culturing microalgae process is easy to control; But also there is following deficiency in closed reactor: 1, because algae liquid receives illumination effect, absorb a large amount of heats, cause the inside reactor temperature to rise; Therefore breeding process needs a large amount of heat-exchange equipments, and energy consumption is big; 2, closed reactor need install the artificial lighting system additional usually, has also increased energy consumption; 3, the closed reactor inwall is easily by little algae pollution, and easy to clean is not difficult to realize the serialization of both culturing microalgae; 4, complex structure amplifies difficulty relatively; 5, equipment manufacturing cost is expensive, safeguards and the process cost height.And traditional open formula reactor drum mainly is a runway pond reactor drum; Be characterized in the reactor water placing flat; Nutrient solution mainly leans on the rotation of paddle wheel or pivot arm to realize circulation; It is cheap, and care and maintenance is easy, but has that frond concentration is lower, the carbonic acid gas utilization ratio is low, production efficiency is low, be prone to by shortcomings such as alien species pollution such as zooplankton, other little algaes in the breeding process.
Summary of the invention
The technical problem that the present invention will solve is to provide a kind of reactor drum that is used for microalgae outdoor breeding; Through improving the carbonic acid gas utilization ratio, realizing that the continuous or semi continuous operation of both culturing microalgae improves greatly breed efficient; Be fit to extensive, low-cost cultivating microalgae, solved the easy pollution problem of algae kind in the traditional open formula reactor culturing for specific algae kind and cultural method.
The technical scheme that the present invention solves the problems of the technologies described above employing is, manufactures and designs a kind of reactor drum that is used for microalgae outdoor breeding, comprises being arranged at outdoor reactor unit, fresh algae liquid transfer lime and carbonic acid gas transfer lime; Comprise in addition:
The microalgae harvesting system is connected in the downstream end of said reactor unit, is used for when frond concentration reaches requirement results and separates little algae, makes the little algae in the reactor unit keep optimum growh density;
Algae liquid return line, its input aperture connects the downstream end of said reactor unit, and its delivery port connects said fresh algae liquid transfer lime, is used for when frond concentration reaches requirement, its conveying not being back to said fresh algae liquid transfer lime and mixing with fresh algae liquid;
The stepped structure of said reactor unit; Falling the power unit that disappears from upstream extremity to downstream end by a plurality of water that reduce step by step links to each other; Said water falls the convex overflow weir that is provided with of the unitary upstream extremity of the power of disappearing, and borders on the river mutually and falls the ccontaining recess that forms algae liquid between the unitary overflow weir of the power of disappearing;
The outlet of said fresh algae liquid transfer lime connects the upstream extremity of said reactor unit; The algae liquid that contains little algae is mobile from the upstream extremity downstream end of reactor unit; In this process; Algae liquid is crossed each water and is fallen the unitary overflow weir of the power of disappearing and flow down in the ccontaining recess, and little algae grows along the reactor flow direction gradually, arrive the reactor unit downstream end after; When frond meets the requirements of concentration, separate little algae through said microalgae harvesting system results, when frond does not meet the requirements of concentration through said algae liquid return line be delivered to said fresh algae liquid transfer lime and with the upstream extremity that returns said reactor unit after fresh algae liquid mixes again.
In order to provide micro algae growth required carbonic acid gas, each water falls in the power unit that disappears the sparger that is connected with the carbonic acid gas transfer lime is installed, and said sparger upper edge is provided with a plurality of delivery ports perpendicular to flow direction, can make the more even distribution of carbonic acid gas.
In order to make the algae flow moving, said water falls the unitary bottom surface of the power of disappearing and is directed downwards inclination towards downstream, and the angle between this bottom surface and the horizontal plane is the scope of 0 to 1 degree.Or, being provided with and helping in said water falls the power unit that disappears, form flowing fluid formation device, this fluid forms device can be hydrodynamic equipment.
Said water falls can be provided with the pH electrode of measuring algae liquid pH value in the power unit that disappears, and can regulate the flow of algae liquid and carbonic acid gas in view of the above.
Said reactor unit upstream extremity can make the inner little algae more even distribution of reactor unit along evenly being provided with a plurality of weirs perpendicular to flow direction, effectively raises the growth velocity of little algae.The downstream end of said reactor unit is provided with the diversion trench that is used for the algae liquid that carrying current gets off; Be provided with the turbidometer that is used to detect frond concentration in this diversion trench; And being connected said microalgae harvesting system and algae liquid return line with 3-way valve through pipe connecting, said pipe connecting is provided with transferpump.
Said fresh algae liquid transfer lime, algae liquid return line, pipe connecting and connect on the carbonic acid gas transfer lime that each water falls sparger in the power unit that disappears and be equipped with the big mini controller of flow.
Said reactor unit can be provided with a plurality of; These a plurality of reactor units can be connected in series; Promptly; The downstream end of previous reactor unit is connected the upstream extremity of next reactor unit through pipe connecting with transferpump, the outlet of said fresh algae liquid transfer lime connects the upstream extremity of first reactor unit, and the downstream end of last reactor unit connects said microalgae harvesting system and algae liquid return line.
Or a plurality of reactor units are connected in parallel, that is, the upstream extremity of each reactor unit all is connected with the outlet of said fresh algae liquid transfer lime, and the downstream end of each reactor unit connects said microalgae harvesting system and algae liquid return line through pipe connecting.
When a plurality of reactor units linked together, wherein the less unitary downstream end of partial reaction device of treatment capacity was connected with buffer container.
Compare with prior art, the reacting appliance that is used for microalgae outdoor breeding of the present invention has following advantage:
(1) since in the reactor unit flow pattern of algae liquid be that staged flows, current fall after the unitary overflow weir of the power of disappearing falls from each water, can rear region form hydraulic jump on the weir; Can form violent turbulent flow in the hydraulic jump zone, have a large amount of mass exchange, make the frond more even distribution; Avoid the frond sedimentation; The mass transfer zone of carbonic acid gas and algae liquid can be set at this, increase the specific absorption of carbonic acid gas greatly, improve speed of reaction.
(2) make the algae kind along the growth of reactor unit axis, behind the arrival reactor bottom, promptly reach higher concentration,, can realize continuously/semi continuous operation through the harvesting system results.
(3) for the algae kind of being screened, through the flow of control algae flow velocity, pH value and carbonic acid gas and algae liquid, guarantee the population advantage of algae kind in reactor drum, can effectively avoid the severe contamination of algae kind.
(4) owing to fall the sparger that the power unit of disappearing has installed carbonic acid gas additional, strengthened the utilization ratio of dioxide gas greatly, effectively reduced cost at each water.
(5) reactor unit can be through several snubber series, parallel and series-parallel connection together, thereby effectively improve the treatment capacity and the processing efficiency of total system.
(6) simple in structure, energy consumption is low, is easy to enlarge produce.
Description of drawings
Fig. 1 is used for the two dimensional structure synoptic diagram of the reactor drum of microalgae outdoor breeding for the present invention;
Fig. 2 is used for the vertical structure synoptic diagram of the reactor drum of microalgae outdoor breeding for the present invention;
Fig. 3 falls the structural representation of the power unit 11 that disappears for said water;
Fig. 4 is the linearly synoptic diagram during type of said reactor unit;
Synoptic diagram when Fig. 5 is the curved type of said reactor unit;
Synoptic diagram when Fig. 6 is the U type for said reactor unit;
Fig. 7 is the synoptic diagram of said reactor unit when S-type;
Synoptic diagram when Fig. 8 is the Back Word type for said reactor unit;
Fig. 9 is the said reactor unit synoptic diagram during type in the shape of a spiral.
Embodiment
Do further to detail below in conjunction with the most preferred embodiment shown in the accompanying drawing.
As depicted in figs. 1 and 2, the reactor drum that is used for microalgae outdoor breeding of the present invention comprises: place outdoor reactor unit 1, algae liquid transfer lime, carbonic acid gas transfer lime 3, microalgae harvesting system 4, transferpump 72 and the big mini controller of flow etc.Wherein, said algae liquid transfer lime comprises fresh algae liquid transfer lime 21, algae liquid return line 22 and pipe connecting 23 again.
As depicted in figs. 1 and 2; Said reactor unit 1 stepped structure; Falling the power unit 11 that disappears from upstream extremity to downstream end by a plurality of water that reduce step by step links to each other; Each water falls the convex overflow weir 12 that is provided with of upstream extremity of the power unit 11 that disappears, and borders on the river mutually and forms the ccontaining recess of algae liquid between the overflow weir 12 that falls the power unit 11 that disappears.Because little algae cultured continuously needs continuously to replenish carbonic acid gas; The mode that adopts in the present embodiment is: in each water falls the power unit 11 that disappears, be equipped with the sparger 31 that is connected with carbonic acid gas transfer lime 3; These sparger 31 upper edges evenly are provided with the delivery port of a plurality of carbonic acid gas perpendicular to flow direction, can make the more even distribution of carbonic acid gas.Certainly can also adopt other mode of movement; Earlier in a gas-liquid mass transfer device, carry out after the gas-liquid exchange input reactor unit 1 upstream extremity again like algae liquid with carbonic acid gas; Additional carbonic acid gas both can use dioxide gas; Also can use liquid that is rich in carbonic acid gas etc., the present invention is not limited to upper type.In addition, each water falls and is equipped with the pH electrode 51 that is used to measure algae liquid pH value in the power unit 11 that disappears, and can regulate the flow of algae liquid and carbonic acid gas in view of the above.Said reactor unit 1 upstream extremity helps little algae along reactor unit 1 width uniform distribution along evenly being provided with a plurality of weirs 13 perpendicular to flow direction.The downstream end of said reactor unit 1 is provided with the diversion trench 15 that is used for the algae liquid that carrying current gets off, and is provided with the turbidometer 52 that is used to detect frond concentration in this diversion trench 15.
In order to make the algae liquid in it form mobile; The bottom surface that said water falls the power unit 11 that disappears is directed downwards inclination towards downstream; Angle between this bottom surface and the horizontal plane is the scope of 0 to 1 degree; Specifically set according to the needed growth time of different algae kinds, utilize the effect of gravity to make the algae liquid in it form mobile; Or the bottom surface that said water falls the power unit 11 that disappears is horizontally disposed with, and fluid is installed within it formed device, like hydrodynamic equipment such as impeller pumps.
As shown in Figure 3, after the overflow weir 12 that current 9 fall the power unit 11 that disappears from each water falls, can rear region form hydraulic jump on the weir; Can form violent turbulent flow 91 in the hydraulic jump zone, have a large amount of mass exchange, and avoid the frond sedimentation; Therefore at this said sparger 31 is set; Form the mass transfer zone of carbonic acid gas and algae liquid, can make the specific absorption of carbonic acid gas reach best, improve speed of reaction.According to factors such as the required growth time of production capacity, selected algae kind, best pH value, the depth of water, carbon dioxide-depleted amounts; Those skilled in the art can design the length L and the width B (Fig. 1) of said reactor unit 1; The angle of inclination i of the width δ of overflow weir 12 end faces, height P1 and P2, weir sidewall; The discrepancy in elevation Δ Z of adjacent overflow weir 12 and apart from numerical value such as S just no longer details at this.
Like Fig. 1 or shown in Figure 2; The upstream extremity of the outlet ligation device unit 1 of said fresh algae liquid transfer lime 21; The diversion trench 15 of said reactor unit 1 downstream end connects a 3-way valve 71 through pipe connecting 23; These 3-way valve 71 two other interfaces connect microalgae harvesting system 4 and algae liquid return line 22 respectively, on the said pipe connecting 23 transferpump 72 are installed, and the other end of said algae liquid return line 22 connects said fresh algae liquid transfer lime 21.The big mini controller of said flow comprises: be installed in valve 24 and under meter 25 on the fresh algae liquid transfer lime 21; Be installed in the under meter 38 on the algae liquid return line 22; Be installed in the under meter 36 on the pipe connecting 23; Be installed in the under meter 37 between 3-way valve 71 and the microalgae harvesting system 4; Be installed in valve 34 and under meter 35 on carbonic acid gas transfer lime 3 main lines; And be installed in the valve 32 and under meter 33 that connects on carbonic acid gas transfer lime 3 branch roads that each water falls spargers 31 in the power unit 11 that disappears.Can control the flow of algae liquid and carbonic acid gas according to the detected algae liquid pH value of coming of each pH electrode and microalgae harvesting situation and through the big mini controller of flow respectively.
The algae liquid that contains little algae flows from upstream extremity and the downstream end that the outlet of fresh algae liquid transfer lime 21 inputs to reactor unit 1; In this process; Algae liquid is crossed that overflow weir 12 that water falls the power unit 11 that disappears flows down in the ccontaining recess and is carried out the gas-liquid exchange with carbonic acid gas from said sparger 31 outputs, and little algae is along the growth of reactor flow direction, arrive the diversion trench 15 of reactor unit 1 downstream end after; When frond meets the requirements of concentration; Said microalgae harvesting system 4 results are separated little algae, and effective separation method such as that said microalgae harvesting system 4 can adopt is centrifugal, micro-filtration, foam separation are isolated little algae, but are not limited to above-mentioned separation method; And when frond does not meet the requirements of concentration; Algae liquid is delivered to said algae liquid return line 22 through pipe connecting 23 and transferpump 72, and should backflow algae liquid after said fresh algae liquid transfer lime 21 converges and the upstream extremity that returns said reactor unit 1 after fresh algae liquid mixes again.
Said reactor unit 1 can be designed to multiple shape; Linear pattern as shown in Figure 4, shaped form as shown in Figure 5, U type as shown in Figure 6, S type as shown in Figure 7, Back Word type as shown in Figure 8 or spiral as shown in Figure 9 etc., but be not limited to above-mentioned shape.In addition; In order to improve treatment capacity and processing efficiency, can also be together with a plurality of reactor unit 1 series, parallel or series-parallel connection, at this moment; When the reactor unit that is connected 1 treatment capacity was inconsistent, the downstream end of the reactor unit 1 that the partially disposed amount is less also was connected with buffer container.When series connection; The downstream end of previous reactor unit 1 is connected the upstream extremity of next reactor unit 1 with transferpump through pipe connecting; The outlet of said fresh algae liquid transfer lime 21 connects the upstream extremity of first reactor unit 1, and the downstream end of last reactor unit 1 is connected said microalgae harvesting system 4 and algae liquid return line 22 through transferpump with 3-way valve.When parallel connection; The upstream extremity of each reactor unit 1 all is connected with the outlet of said fresh algae liquid transfer lime 21, and the downstream end of each reactor unit 1 links together through pipe connecting after transferpump is connected said microalgae harvesting system 4 and algae liquid return line 22 with 3-way valve.
In addition; When reactor drum of the present invention at arctic weather or when being installed in the high latitude cold district; Can one heat-exchanger rig be set in said reactor unit 1 bottom; This heat-exchanger rig can be the waste gas heat exchange tube that links to each other with thermal power plant waste gas, makes full use of its heat energy and makes the algae liquid in the reactor unit 1 keep certain temperature.

Claims (10)

1. a reactor drum that is used for microalgae outdoor breeding comprises being arranged at outdoor reactor unit (1), fresh algae liquid transfer lime (21) and carbonic acid gas transfer lime (3); It is characterized in that, also comprise:
Microalgae harvesting system (4) is connected in the downstream end of said reactor unit (1), is used for when frond concentration reaches requirement results and separates little algae;
Algae liquid return line (22); Its input aperture connects the downstream end of said reactor unit (1); Its delivery port connects said fresh algae liquid transfer lime (21), is used for when frond concentration reaches requirement, its conveying not being back to said fresh algae liquid transfer lime (21) and mixing with fresh algae liquid;
The downstream end of said reactor unit (1) is provided with the diversion trench (15) that is used for the algae liquid that carrying current gets off, and is provided with the turbidometer (52) that is used to detect frond concentration in this diversion trench (15);
The stepped structure of said reactor unit (1); Falling the power unit (11) that disappears from upstream extremity to downstream end by a plurality of water that reduce step by step links to each other; Said water falls the convex overflow weir (12) that is provided with of upstream extremity of the power unit (11) that disappears, and borders on the river mutually and forms the ccontaining recess of algae liquid between the overflow weir (12) that falls power unit (11) that disappear;
The outlet of said fresh algae liquid transfer lime (21) connects the upstream extremity of said reactor unit (1); The algae liquid that contains frond is mobile from the upstream extremity downstream end of reactor unit (1); In this process; Algae liquid is crossed the overflow weir (12) that each water falls power unit (11) that disappear and is flowed down in the ccontaining recess; Frond is grown along the reactor unit flow direction gradually; After arriving reactor unit (1) downstream end, when frond meets the requirements of concentration, separate little algae through said microalgae harvesting system (4) results, when frond does not meet the requirements of concentration through said algae liquid return line (22) be delivered to said fresh algae liquid transfer lime (21) and with the upstream extremity that returns said reactor unit (1) after fresh algae liquid mixes again.
2. the reactor drum that is used for microalgae outdoor breeding according to claim 1; It is characterized in that: each water falls the sparger (31) that is connected with carbonic acid gas transfer lime (3) is installed in the power unit (11) that disappears, and said sparger (31) upper edge is provided with a plurality of delivery ports perpendicular to flow direction.
3. the reactor drum that is used for microalgae outdoor breeding according to claim 1 is characterized in that: it is moving that the bottom surface that said water falls the power unit (11) that disappears is directed downwards the algae flow that tilts to make in it towards downstream, and the angle between this bottom surface and the horizontal plane is the scope of 0 to 1 degree.
4. the reactor drum that is used for microalgae outdoor breeding according to claim 1 is characterized in that: also include and help in said water falls the power unit (11) that disappears, form flowing fluid formation device, it is hydrodynamic equipment that this fluid forms device.
5. according to each described reactor drum that is used for microalgae outdoor breeding of claim 1 to 4, it is characterized in that: said water falls the pH electrode (51) that is provided with measurement algae liquid pH value in the power unit (11) that disappears.
6. the reactor drum that is used for microalgae outdoor breeding according to claim 2; It is characterized in that: said reactor unit (1) upstream extremity is along evenly being provided with a plurality of weirs (13) perpendicular to flow direction; Said diversion trench (15) is connected said microalgae harvesting system (4) and algae liquid return line (22) through pipe connecting (23) with 3-way valve (71), said pipe connecting (23) is provided with transferpump (72).
7. the reactor drum that is used for microalgae outdoor breeding according to claim 6 is characterized in that: said fresh algae liquid transfer lime (21), algae liquid return line (22), pipe connecting (23) and connect on the carbonic acid gas transfer lime (3) that each water falls the interior sparger in power unit (11) (31) that disappears and be equipped with the big mini controller of flow.
8. the reactor drum that is used for microalgae outdoor breeding according to claim 1; It is characterized in that: said reactor unit (1) has a plurality of; These a plurality of reactor units (1) are connected in series; Promptly; The downstream end of previous reactor unit (1) is connected the upstream extremity of next reactor unit (1) through pipe connecting with transferpump, the outlet of said fresh algae liquid transfer lime (21) connects the upstream extremity of first reactor unit (1), and the downstream end of last reactor unit (1) connects said microalgae harvesting system (4) and algae liquid return line (22).
9. the reactor drum that is used for microalgae outdoor breeding according to claim 1; It is characterized in that: said reactor unit (1) has a plurality of; These a plurality of reactor units (1) are connected in parallel; That is, the upstream extremity of each reactor unit (1) all is connected with the outlet of said fresh algae liquid transfer lime (21), and the downstream end of each reactor unit (1) connects said microalgae harvesting system (4) and algae liquid return line (22) through pipe connecting.
10. according to Claim 8 or the 9 described reactor drums that are used for microalgae outdoor breeding, it is characterized in that: the downstream end of partial reaction device unit (1) is connected with buffer container.
CN 200910106956 2009-05-06 2009-05-06 Reactor for microalgae outdoor breeding Expired - Fee Related CN101550393B (en)

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