CN1472306A - Unitary continuous aerating collecting carbon-complementing device and method for culturing microalgae - Google Patents

Unitary continuous aerating collecting carbon-complementing device and method for culturing microalgae Download PDF

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Publication number
CN1472306A
CN1472306A CNA011422971A CN01142297A CN1472306A CN 1472306 A CN1472306 A CN 1472306A CN A011422971 A CNA011422971 A CN A011422971A CN 01142297 A CN01142297 A CN 01142297A CN 1472306 A CN1472306 A CN 1472306A
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carbon
algae
continuously
mend
little algae
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Inventor
威 丛
丛威
曾文炉
蔡昭玲
欧阳藩
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Institute of Process Engineering of CAS
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Institute of Process Engineering of CAS
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Priority to CNA011422971A priority Critical patent/CN1472306A/en
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Abstract

A process and apparatus for large-scale culture of microalgae, continuously supplementing carbon and collecting microaglae by air floating features that the flue fume is used for supplementing carbon and air-floating collection. Its advantages are high output rate and low cost.

Description

A kind of continuous aerating collecting, mend the apparatus and method that carbon is cultivated little algae continuously
Technical field
The present invention relates to the cultured continuously method of a kind of little algae, especially a kind of continuous aerating collecting, mend the apparatus and method that carbon is cultivated little algae continuously.
Background technology
Nutritive ingredients (as spirulina) such as little algae rich in proteins, polysaccharide, unsaturated fatty acids can be used for food, medical aspect; Contain multiple pigment (as Porphyridium cruentum), can be used for makeup, biotechnology and medical diagnosis; Can accumulate hydrocarbon polymer in a large number, hydrocarbons content can account for 49% (as Dunaliella salina) of dry weight, is directly used in generating; Algae can fixed nitrogen slightly for certain, can be used as Natural fertilizers (as anabena), can be used as high-quality bait by nutritional control; Little algae of some genus also contains microbiotic and toxin, has pharmaceutical use.Little algae has become the raw material of food, healthcare products, medicine and fine chemistry industry, demonstrates huge market potential.
From present present situation, still there is suitable difficulty in the industrialization of little algae, and is main because the problem of through engineering approaches does not have fine solution.Most widely used in little algae is cultivated is open pond culture systems, and it possesses skills simply, invests characteristics such as cheap, has obtained good effect in the large scale culturing of spirulina, chlorella and salt algae.But there is the culture condition instability simultaneously in it, and the unit volume productive rate is low, and downstream processing cost height is difficult for keeping shortcomings such as purebred cultivation.And the bioreactor that is used for little algae cultivation still is in the development stage, does not form industrialized scale.The factor that restricts little algae industrialized development at first is that culture density is low, usually at the order of magnitude of lg/L, can reach the magnitude of 10-15g/L after the optimization technology, than low 1-2 the order of magnitude of land microbial fermentation, cause cost high, wherein the restriction of light intensity and carbon source is a major cause; Next is that the preliminary working of existing little algae is backward in technique.The workload of manually gathering that adopts is big at present, and the efficient of gathering is low, has increased the cultivation cost greatly, is unfavorable for forming scale production.
Adding an amount of carbon source in nutrient solution is to improve the effective means of culture density.Main in the present open culture systems by adding a large amount of NaHCO 3Mend carbon.Studies show that NaHCO 3Benefit carbon effect not as CO 2, and add the pH rising that carbonate can make culture system, and increase cost.But directly logical CO in open pond 2, because gas-liquid contact time is short, mass-transfer efficiency is low, causes CO 2Significant wastage.
Summary of the invention
The objective of the invention is to: if energy timely collecting in the process of growth of little algae, the density that can eliminate to a certain extent on the one hand in little algae breeding suppresses, keep high-density culture, can alleviate the relaxation phenomenon of light on the other hand, and in good time gathering continuously also helps industrialization to successional requirement.With having dissolved CO 2Dissolved air water (rare algae liquid) gather, can when gathering, realize efficiently mending carbon, thereby provide a kind of continuous aerating collecting, mend the apparatus and method that carbon is cultivated little algae continuously.
A kind of continuous aerating collecting provided by the invention, mend carbon continuously and cultivate the employed device of method of little algae and mainly comprise: source of the gas and pressure exerting device, saturex, air supporting tower (bubble carried separator) and micro algae culturing device.Its main process and principle are: CO 2(dust-removing flue gas) and Air mixing gas enter the saturex dissolving through the pressurization back by pipeline, (being designated hereinafter simply as dissolved air water) reaches capacity to make water in the saturex (from rare algae liquid of mending carbon culture of isolated device), this dissolved air water enters non-pressurized benefit carbon culture of isolated device one air supporting tower along pipeline through water release device, with the algae liquid thorough mixing of gathering along treating in the pipeline access to plant, the contact of generation turbulent diffusion, (belong to the liquid-liquid mass transfer to the quick mass transfer of algae liquid, resistance to mass transfer is little), realize the efficient carbon of mending; Meanwhile, form a large amount of microbubbles after the gas step-down of another part solvation, these microbubbles adhere to mutually with frustule, and come-up reaches the isolating purpose of cell concentration, and spissated algae liquid and part tail gas are respectively by pipeline and discharge.Get back in the compressed air source unit by pipe-line transportation behind bubble process gas one water separator that rises in the device, through recycling after the pressurization again.In addition, CO 2Solubleness is bigger than air in algae liquid, and air forms microbubble with preferential desorb during release of pressure, implements cellular segregation and the CO of solvation 2Main effect is to mend carbon, and therefore, process has realized reasonable and economical.Be rich in CO 2Rare algae liquid enter little algae cultivation pool through pipeline, or return in the saturex along pipeline, participate in circulation next time.
This process is operate continuously, waits to gather, mend the continuous injection device of high-density algae liquid of carbon, and discharge has implemented to mend carbon and rare algae liquid of gathering continuously simultaneously, passes back into cultivation pool.Synoptic diagram is as follows:
Effect of the present invention: because the present invention utilizes continuous air-dissolved (air and carbonic acid gas, from stack gas) air flotation technology, the frustule of in the air supporting tower, gathering continuously, simultaneously carbon dioxide dissolved is replenished to algae liquid, both utilized stack gas, save a large amount of inorganic carbon sources, improved the mass-transfer efficiency (efficient of liquid liquid mass transfer is far above gas-liquid mass transfer) of mending carbon usually with carbonic acid gas again.The present invention can remove the restriction of carbon source deficiency to growth effectively, and simultaneously, timely collecting in the process of growth of little algae also can alleviate the decay that little algae breeds the light intensity that causes to a certain extent, helps having improved culture efficiency.
Description of drawings Fig. 1 is continuously that the bubble year little algae of separation is mended the carbon system simultaneously
Specific embodiments
Embodiment 1
A kind of continuous aerating collecting, mend carbon continuously and cultivate the employed equipment of method of little algae and mainly comprise: source of the gas and pressure exerting device 1, saturex 2, air supporting tower (bubble carried separator) 3 and micro algae culturing device (4).Its main process and principle are: CO 2(dust-removing flue gas) and Air mixing gas enter saturex (2) dissolving through the pressurization back by pipeline 5, (being designated hereinafter simply as dissolved air water) reaches capacity to make water in the saturex (from rare algae liquid of mending carbon culture of isolated device), this dissolved air water enters non-pressurized benefit carbon culture of isolated device one air supporting tower (3) along pipeline through water release device 6, with the algae liquid thorough mixing of gathering along treating in pipeline 8 accesss to plant, the contact of generation turbulent diffusion, (belong to liquid-liquid mass transfer to the quick mass transfer of algae liquid, resistance to mass transfer is little), realize the efficient carbon of mending; Meanwhile, form a large amount of microbubbles after the gas step-down of another part solvation, these microbubbles adhere to mutually with frustule, and come-up reaches the isolating purpose of cell concentration, and spissated algae liquid and part tail gas are discharged by pipeline 10 and 11 respectively.Get back in the compressed air source unit by pipeline 9 conveyings behind the bubble process air-water separator that rises in the device, through recycling after the pressurization again.In addition, CO 2Solubleness is bigger than air in algae liquid, and air forms microbubble with preferential desorb during release of pressure, implements cellular segregation and the CO of solvation 2Main effect is to mend carbon, and therefore, process has realized reasonable and economical.Be rich in CO 2Rare algae liquid enter little algae cultivation pool through pipeline 7, or return in the saturex along pipeline 12, participate in circulation next time.
This process is operate continuously, waits to gather, mend the continuous injection device of high-density algae liquid of carbon, and discharge has implemented to mend carbon and rare algae liquid of gathering continuously simultaneously, passes back into cultivation pool.Synoptic diagram is as follows:

Claims (2)

  1. A continuous aerating collecting, mend the device that carbon is cultivated little algae continuously, comprise source of the gas and pressure exerting device, saturex, air supporting tower (bubble carried separator) and micro algae culturing device.
  2. 2. an application rights requires 1 described continuous aerating collecting, mends carbon continuously and cultivate the method that the device of little algae is cultivated little algae, it is characterized in that: operate continuously, wait to gather, mend the continuous injection device of high-density algae liquid of carbon, discharge has implemented to mend carbon and rare algae liquid of gathering continuously simultaneously, pass back into cultivation pool, realize the integrated and serialization that little algae is cultivated, mends carbon, aerating collecting continuously.
CNA011422971A 2001-09-27 2001-09-27 Unitary continuous aerating collecting carbon-complementing device and method for culturing microalgae Pending CN1472306A (en)

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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007068191A1 (en) * 2005-12-12 2007-06-21 Institute Of Process Engineering, Chinese Academy Of Sciences A carbon supply device for cultivating miro algae in large and its application method and use
CN102134553A (en) * 2010-01-27 2011-07-27 中国科学院过程工程研究所 Tubular photobioreactor and system and method for culturing microalgae cells
CN101457201B (en) * 2008-11-22 2011-08-10 中国海洋大学 Lining rod type bubble separation apparatus
CN101519634B (en) * 2009-03-27 2012-02-01 兆凯生物工程研发中心(深圳)有限公司 Open type biological reactor for microalgae cultivation and microalgae cultivation method
CN101550393B (en) * 2009-05-06 2012-05-02 兆凯生物工程研发中心(深圳)有限公司 Reactor for microalgae outdoor breeding
CN105018331A (en) * 2015-07-16 2015-11-04 中国海洋大学 Multidirectional-turbulence and efficient-mixing novel runway pond microalgae cultivation system

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007068191A1 (en) * 2005-12-12 2007-06-21 Institute Of Process Engineering, Chinese Academy Of Sciences A carbon supply device for cultivating miro algae in large and its application method and use
US8241634B2 (en) 2005-12-12 2012-08-14 Institute Of Process Engineering, Chinese Academy Of Sciences Carbon supply device for cultivating micro-algae in large scale and its application method and use
CN101457201B (en) * 2008-11-22 2011-08-10 中国海洋大学 Lining rod type bubble separation apparatus
CN101519634B (en) * 2009-03-27 2012-02-01 兆凯生物工程研发中心(深圳)有限公司 Open type biological reactor for microalgae cultivation and microalgae cultivation method
CN101550393B (en) * 2009-05-06 2012-05-02 兆凯生物工程研发中心(深圳)有限公司 Reactor for microalgae outdoor breeding
CN102134553A (en) * 2010-01-27 2011-07-27 中国科学院过程工程研究所 Tubular photobioreactor and system and method for culturing microalgae cells
CN102134553B (en) * 2010-01-27 2013-11-06 中国科学院过程工程研究所 Tubular photobioreactor and system and method for culturing microalgae cells
CN105018331A (en) * 2015-07-16 2015-11-04 中国海洋大学 Multidirectional-turbulence and efficient-mixing novel runway pond microalgae cultivation system

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