CN101541954B - 含有人脐带血衍生的间充质干细胞的组合物诱导神经前体细胞或神经干细胞分化和增殖为神经细胞的用途 - Google Patents

含有人脐带血衍生的间充质干细胞的组合物诱导神经前体细胞或神经干细胞分化和增殖为神经细胞的用途 Download PDF

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CN101541954B
CN101541954B CN2007800440974A CN200780044097A CN101541954B CN 101541954 B CN101541954 B CN 101541954B CN 2007800440974 A CN2007800440974 A CN 2007800440974A CN 200780044097 A CN200780044097 A CN 200780044097A CN 101541954 B CN101541954 B CN 101541954B
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cord blood
cell
stem cell
cells
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CN101541954A (zh
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吴元一
梁允瑄
蒋钟旭
崔秀真
金珠渊
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Medipost Co Ltd
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CN2007800440974A 2006-11-30 2007-11-29 含有人脐带血衍生的间充质干细胞的组合物诱导神经前体细胞或神经干细胞分化和增殖为神经细胞的用途 Active CN101541954B (zh)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US86787506P 2006-11-30 2006-11-30
US60/867,875 2006-11-30
KR10-2006-0120479 2006-12-01
KR1020060120479 2006-12-01
KR1020060120479A KR100959995B1 (ko) 2006-12-01 2006-12-01 인간 제대혈 유래 간엽 줄기세포를 유효성분으로 포함하는,신경전구세포 또는 신경줄기세포의 신경세포로의 분화 및증식 유도용 조성물
PCT/KR2007/006084 WO2008066330A1 (en) 2006-11-30 2007-11-29 Use of a composition contaning human umbilical cord blood-derived mesenchymal stem cell for inducing differentiation and proliferation of neural precursor cells or neural stem cells to neural cells

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CN101541954A CN101541954A (zh) 2009-09-23
CN101541954B true CN101541954B (zh) 2012-12-26

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US (2) US9387226B2 (https=)
EP (1) EP2099901B1 (https=)
JP (2) JP2010511381A (https=)
CN (1) CN101541954B (https=)
AU (1) AU2007326171B2 (https=)
CA (1) CA2669304C (https=)
MX (1) MX2009005496A (https=)
WO (1) WO2008066330A1 (https=)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20200011604A (ko) 2008-08-20 2020-02-03 안트로제네시스 코포레이션 개선된 세포 조성물 및 그의 제조 방법
CN101831401A (zh) * 2010-04-23 2010-09-15 天津昂赛细胞基因工程有限公司 体外诱导间充质干细胞分化为神经干细胞的方法
US11286463B2 (en) 2012-03-08 2022-03-29 Advanced ReGen Medical Technologies, LLC Reprogramming of aged adult stem cells
US9994814B2 (en) 2013-04-09 2018-06-12 Advanced ReGen Medical Technologies, LLC Method for cellular restoration
CR20160307A (es) 2013-12-20 2016-11-08 Advanced Regen Medical Tech Llc Composiciones para la restauración de células y métodos para la preparación y utilización de las mismas
CN113354744A (zh) 2013-12-20 2021-09-07 弗雷德哈钦森癌症研究中心 带标签的嵌合效应分子及其受体
US10772911B2 (en) 2013-12-20 2020-09-15 Advanced ReGen Medical Technologies, LLC Cell free compositions for cellular restoration and methods of making and using same
WO2016068616A1 (ko) * 2014-10-29 2016-05-06 차의과학대학교 산학협력단 C3 또는 c1r 보체를 분비하는 태반 유래 세포 및 이를 포함하는 조성물
EP3288570A4 (en) 2015-04-29 2018-11-21 Fred Hutchinson Cancer Research Center Modified stem cells and uses thereof
TWI589698B (zh) 2015-07-22 2017-07-01 中國醫藥大學 間質幹細胞、其純株化擴張之方法、其分離方法及其應用
CN106701668B (zh) * 2015-07-22 2020-12-29 中国医药大学 间质干细胞、其纯株化扩张的方法及其应用
US11110129B2 (en) 2015-07-22 2021-09-07 China Medical University Method for treating multiple sclerosis
ITUB20159750A1 (it) * 2015-12-30 2017-06-30 Alfredo Gorio Metodo per la promozione ed il miglioramento delle proprieta? del tessuto adiposo, tessuto e cellule ottenute tramite detto metodo
CA3022275A1 (en) 2016-04-28 2017-11-02 Sapporo Medical University Synapse formation agent
EP3436081A4 (en) 2016-04-29 2019-07-24 Advanced Regen Medical Technologies, LLC Microrna compositions and methods of making and using same
JP7034949B2 (ja) * 2016-05-25 2022-03-14 テルモ ビーシーティー、インコーポレーテッド 細胞の増殖
US10717981B2 (en) 2018-01-18 2020-07-21 Advanced ReGen Medical Technologies, LLC Therapeutic compositions and methods of making and using the same
WO2019237106A1 (en) * 2018-06-08 2019-12-12 University Of Virginia Patent Foundation Compositions and methods for treating stroke
JPWO2022004822A1 (https=) * 2020-06-30 2022-01-06

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1302534A1 (en) * 2000-06-26 2003-04-16 Renomedix Institute Inc. Cell fraction containing cells capable of differentiating into neural cells

Family Cites Families (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7354588B1 (en) 1987-05-04 2008-04-08 Dana Farber Cancer Institute Functional derivatives of ICAM-1 with altered ability to bind LFA-1 or HRV
US5733542A (en) 1990-11-16 1998-03-31 Haynesworth; Stephen E. Enhancing bone marrow engraftment using MSCS
US5837576A (en) 1997-10-31 1998-11-17 Vanguard International Semiconductor Corporation Method for forming a capacitor using a silicon oxynitride etching stop layer
US6900299B1 (en) 1999-03-11 2005-05-31 University Of South Florida Interrupting the interaction of intercellular adhesion molecule-1 and respiratory syncytial virus for prevention and treatment of infection
EP2177228A1 (en) 1999-03-12 2010-04-21 The Board Of Trustees Of The Leland Stanford Junior University DNA vaccination for treatment of autoimmune disease
US6372494B1 (en) 1999-05-14 2002-04-16 Advanced Tissue Sciences, Inc. Methods of making conditioned cell culture medium compositions
US20040208858A1 (en) 2000-04-12 2004-10-21 Gihan Tennekoon Therapeutic uses for mesenchymal stromal cells
US20040037775A1 (en) 2000-08-01 2004-02-26 Siahaan Teruna J. Leukocyte internalized peptide-drug conjugates
US7560280B2 (en) * 2000-11-03 2009-07-14 Kourion Therapeutics Gmbh Human cord blood derived unrestricted somatic stem cells (USSC)
KR100449141B1 (ko) 2001-04-19 2004-09-21 (주)라이프코드 간엽 간세포를 신경세포로 분화시키는 방법
EP1483371B1 (en) 2002-02-19 2007-06-27 Medipost, Co., Ltd. Isolation and culture-expansion methods of mesenchymal stem/progenitor cells from umbilical cord blood, and differentiation method of umbilical cord blood-derived mesenchymal stem/progenitor cells into various mesenchymal tissues
US20090192105A1 (en) 2002-02-20 2009-07-30 Sirna Therapeutics, Inc. RNA INTERFERENCE MEDIATED INHIBITION OF INTERCELLULAR ADHESION MOLECULE (ICAM) GENE EXPRESSION USING SHORT INTERFERING NUCELIC ACID (siNA)
KR100519227B1 (ko) 2002-08-17 2005-10-07 서해영 간엽 줄기세포를 신경세포로 분화시키는 방법
US20040203142A1 (en) 2003-04-14 2004-10-14 Reliance Life Sciences Pvt. Ltd. Growth of neural precursor cells using umbilical cord blood serum and a process for the preparation thereof for therapeutic purposes
EP1674566B1 (en) 2003-09-09 2011-04-13 Keio University Method of promoting subsistence and/or proliferation of neural stem cell and promoting extension of neurite, promoter therefor, pharmaceutical composition containing neural stem cell, method of assay and method of screening
US20060094064A1 (en) 2003-11-19 2006-05-04 Sandip Ray Methods and compositions for diagnosis, stratification, and monitoring of alzheimer's disease and other neurological disorders in body fluids
EP1767617A1 (en) 2005-09-26 2007-03-28 Letizia Mazzini Mesenchymal stem cells isolation and expansion method and uses thereof
EP1951665A1 (en) 2005-11-14 2008-08-06 Dominion Pharmakine S.L. Novel inhibitors of the lfa-1/icam-1 interaction, and uses thereof
US20070253931A1 (en) 2006-01-12 2007-11-01 Osiris Therapeutics, Inc. Use of mesenchymal stem cells for treating genetic diseases and disorders
KR100792184B1 (ko) 2006-05-12 2008-01-07 재단법인서울대학교산학협력재단 개의 제대혈, 태반 및 개 태아의 심장 유래 다분화능 성체줄기세포, 그 제조방법 및 이를 함유하는 세포 치료제
KR20080049562A (ko) 2006-11-30 2008-06-04 가톨릭대학교 산학협력단 제대혈 유래 간엽 줄기세포를 유효성분으로 포함하는 신경손상 질환 치료제
KR20100054711A (ko) 2008-11-14 2010-05-25 메디포스트(주) 간엽 줄기세포 또는 이의 배양액을 포함하는 신경질환의 예방 또는 치료용 조성물

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1302534A1 (en) * 2000-06-26 2003-04-16 Renomedix Institute Inc. Cell fraction containing cells capable of differentiating into neural cells

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
Kadereit, Deeds, Haynesworth et al..Expansion of LTC-ICs and Maintenance of p21 and BCL-2 Expression in Cord Blood CD34 + /CD38 – Early Progenitors Cultured over Human MSCs as a Feeder Layer.《Stem Cells》.2002,第 20 卷573-582. *
Oscar K. Lee et al..Isolation of multipotent mesenchymal stem cells from umbilical cord blood.《Blood》.2003,第 103 卷(第 5 期),1669-1675. *
Yun Kyung Jang et al..Mesenchymal stem cells feeder layer from human umbilical cord blood for ex vivo expanded growth and proliferation of hematopoietic progenitor cells.《Ann Hematol》.2006,第 85 卷212-225. *

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CN101541954A (zh) 2009-09-23
EP2099901A4 (en) 2010-01-13
US9387226B2 (en) 2016-07-12
AU2007326171B2 (en) 2012-11-15
CA2669304C (en) 2016-10-25
US20100074875A1 (en) 2010-03-25
CA2669304A1 (en) 2008-06-05
JP5871865B2 (ja) 2016-03-01
JP2013226159A (ja) 2013-11-07
US20130330306A1 (en) 2013-12-12
MX2009005496A (es) 2009-06-03
WO2008066330A8 (en) 2009-05-28
AU2007326171A1 (en) 2008-06-05
EP2099901A1 (en) 2009-09-16
HK1134688A1 (en) 2010-05-07
EP2099901B1 (en) 2015-09-09
US9439931B2 (en) 2016-09-13
WO2008066330A1 (en) 2008-06-05

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