CN101535796B - 用于评估样品的方法和设备 - Google Patents
用于评估样品的方法和设备 Download PDFInfo
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- CN101535796B CN101535796B CN2006800562498A CN200680056249A CN101535796B CN 101535796 B CN101535796 B CN 101535796B CN 2006800562498 A CN2006800562498 A CN 2006800562498A CN 200680056249 A CN200680056249 A CN 200680056249A CN 101535796 B CN101535796 B CN 101535796B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/251—Colorimeters; Construction thereof
- G01N21/253—Colorimeters; Construction thereof for batch operation, i.e. multisample apparatus
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/21—Polarisation-affecting properties
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/59—Transmissivity
- G01N21/5907—Densitometers
- G01N21/5911—Densitometers of the scanning type
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates
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Abstract
为检测并储存关于光学特性的信息,分析样品连同放在样品前面和后面的滤光器被进行数字扫描。用于执行这种方法的设备具有用于将滤光器放置在分析样品每一侧的装置。所述滤光器可以是偏光器、荧光滤光器等。
Description
本发明涉及一种利用数字扫描设备检测并储存关于分析样品的光学特性的信息的方法,以及一种用于执行该方法的平板扫描仪附属设备。
已知利用平板扫描仪或其它电光设备对分析样品进行评估。在WO89/07255中公开了利用平板扫描仪对关于化学或生物学试验或工序,特别是血液样品分析的信息进行提取。在US 2002/0168784中公开了一种使用平板扫描仪、专门地确定和储存凝集试验的结果的诊断系统。被检测的光学特性为样品及产生的凝集物的荧光、颜色、光散射或特征。
对于所有这些已知方法,优选为微量滴定板形式的样品容器被简单地放置在扫描台上。
现在已经发现可以使用除平板扫描仪之外的附属设备进行其它类型的试验。
因此,本发明关注执行这样的步骤:将滤光器放在每一侧,即沿光的方向位于分析样品的前面和后面,并扫描滤光器之间的样品布置。此外,本发明关注用于通过在分析样品每一侧放置滤光器来对数字扫描仪进行补充的设备。根据本发明较具体的实施例,所述滤光器为偏振滤光器,通过扫描检测并储存的特性为样品的旋光性。
下面参考附图描述本发明的优选实施例。图1示出平板扫描仪附属设备的示意透视图;图2示出图1所示设备的示意截面图;图3示出图1所示设备的顶视图;图4示出显示棱镜功能的细节;图5示出附属设备的可选形式;图6示出利用条码进行样品识别;图7示出用于分析较大物体的实施例;图8示出分析较大物体的另一可能性;图9示出同时分析多个较大物体;图10示出一排较大容器的分析;图11示出分析一排较大容器的可选可能性;图12示出个别滤光器的细节。
如图1至图3所示,包含多个样品井(sample well)2的微量滴定板1被放入用诸如丙烯酸玻璃等全透明材料制成的外壳3中。所述外壳由带有微量滴定板放置于其中的开口5的框架4构成。
框架4具有第一狭缝6和第二狭缝7,第一狭缝6设置在插入的微量滴定板的上部范围之上,第二狭缝7设置在微量滴定板下表面之下。所述两个狭缝用来容纳薄片等形状的滤光器8。
在本实施例中,滤光器8为偏振滤光器或偏光器。为确定一排样品中的个别样品存在或不存在旋光性,偏光器需要被置于不同的相对位置,即平行、交叉或中间状态。在图1中,偏光器被显示为交叉状态。但它们也可以以平行的偏振方向或在任何期望的角度下插入。
如图2的横截面中所示,定位销9可以被置于微量滴定板旁边,以便确保井处在限定的位置。
从图2以及图4更详细的显示还可以看出,由诸如丙烯酸玻璃的全透明材料制成的棱镜10沿微量滴定板的一个短边放置,以引导光从侧面穿过样品,用于例如使样品产生荧光。可以沿微量滴定板的其它边或所有边设置若干棱镜。如图6中所示,棱镜还被用来读取位于微量滴定板侧面用于其识别的条码。条码在图像分析期间被自动处理。
如本领域众所周知的,荧光发光可以在交叉的偏光器之间被测量,或通过适合的荧光滤光器和/或滤色器被测量。即一个或两个偏光器被其它类型的滤光器代替或补充。
如图3的顶视图中所示,在滤光器上设置粘贴物或划痕形式的标记11,用来为图像分析定义所述滤光器的位置。
如图5中所示,本发明另一实施例的外壳12具有凹入的上表面和下表面,以代替狭缝来容纳滤光器。
为进行样品的扫描,外壳连同滤光器以及装入的微量滴定板被放在扫描仪平台上。外壳在扫描仪上的准确定位是通过诸如例如搭扣贴片(hookand loop patches)等方法实现的。可选择地,外壳可以被固定附连到专为这种分析目的使用的扫描仪。
对于需要加热的样品,滤光器被电热毯类型的电加热层补充或代替。这种加热设备的电源可以被放在扫描仪的外面。温度可以用温度传感器或温度调节装置来控制。
除了滤光器或加热盘,标识或辅助薄片或遮盖物可以被放在微量滴定盘上部,以使得能够或便于识别或允许另外的定量和分析。这些薄片或遮盖物可以包含井编号、十字、黑色圆圈或点、网格或全黑背景。这些有助于定量和分析多种特性,诸如表面张力、浊度或气泡的存在。通常这些遮盖物有助于提高对比度。
本设备允许多种消耗品的扫描,消耗品诸如显微镜载玻片、病理学载波片、Terasaki板、任何孔板(well plate)或皮氏培养皿。当然,某些消耗品可能需要合适的转接器(adapter)。同样不需要每次只扫描一个板或消耗品。依据其大小,若干消耗品可以被同时分析。同样可以提供诸如A3的更大的扫描仪以容纳若干SBS尺寸微孔板(micro plate)。
本设备是机械手臂,兼容以利用标准机械手臂自动插入或去除板。每侧都有可用的空间,这还有利于简单地进行手动操作。
根据图7至图12所示的本发明的可选实施例,诸如小瓶或瓶子的较大物体也可以用同样的方式进行扫描。外壳的尺寸当然有所调整。如图7所示,瓶子13被放在扫描台14上。该扫描仪属于使用来自其盖子15的顶部照明的类型。瓶子被置于滤光器16以及棱镜17之间以从不同的侧面进行扫描。瓶子后面的滤光器可以由放大镜补充或代替。滤光器可以是偏光器、荧光滤光器等。也可以使用镜子替代棱镜。
瓶子被扫描的侧面和底部图像19被示意性地显示在扫描台下方。
这可能有助于浮在混浊溶液中的粒子18的分析,包括它们随时间的行为,诸如沉淀动力学。如果使用一块棱镜或镜子,则可以从全部四个侧面扫描瓶子。
如图8中所示,利用只在一边的滤光器16和棱镜或镜子17可以对瓶子进行相似分析。图9示出相似的设置,其中诸如不同尺寸的瓶子20和荧光比色皿21的多个不同物体被同时扫描。图10示出对棱镜或镜子23之间一排相同小瓶22例如浮动粒子等的扫描。如图11所示,相同的设置可以与诸如偏光器或荧光滤光器等滤光器24一起使用。根据图12所示的另一实施例,能够单独旋转的偏光器或荧光滤光器可以被关联到每个小瓶。
随后的图像分析可以包括尺寸表征、结构形态、颜色识别和强度定量,以及细胞分析。
Claims (10)
1.利用包括光源的平板扫描仪来检测并储存关于分析样品光学特性的信息的方法,所述方法包括:将包括所述分析样品的样品消耗品放置到外壳中,将滤光器放置于所述样品的每一侧,即沿光的方向所述分析样品的前面和后面,其中,所述滤光器被容纳在设置在所述外壳中所述样品的每一侧的凹入或狭缝中,将所述外壳放置或固定附连到所述平板扫描仪的扫描台上,并对滤光器之间的样品布置进行扫描。
2.根据权利要求1所述的方法,其中,通过扫描而检测并储存的特性是所述样品的偏振图像。
3.根据权利要求1所述的方法,其中,通过扫描而检测并储存的特性是所述样品的荧光。
4.一种用于利用包括光源的平板扫描仪来检测并储存关于分析样品光学特性的信息的设备,所述设备包括用于将滤光器放置于所述分析样品的每一侧的装置,所述装置包括外壳,所述外壳包括具有所述分析样品的样品消耗品以及设置在所述外壳中所述样品的每一侧用于容纳所述滤光器的凹入或狭缝。
5.根据权利要求4所述的设备,其中,所述滤光器是偏振滤光器。
6.根据权利要求4所述的设备,其中,所述滤光器是荧光滤光器。
7.根据权利要求4所述的设备,其中,所述样品被容纳在微量滴定板的井中。
8.根据权利要求4所述的设备,其中,所述样品被容纳在瓶子中。
9.根据权利要求4所述的设备,其中,棱镜或镜子被布置在样品的一侧或多侧。
10.根据权利要求4到9中任一个所述的设备,其中,所述滤光器中的至少一个被放大镜补充或代替。
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PCT/EP2006/010561 WO2008052587A1 (en) | 2006-11-03 | 2006-11-03 | Method and apparatus for evaluating samples |
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CN101535796B true CN101535796B (zh) | 2013-05-01 |
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US (1) | US20100067008A1 (zh) |
EP (1) | EP2080010A1 (zh) |
JP (1) | JP5265558B2 (zh) |
CN (1) | CN101535796B (zh) |
WO (1) | WO2008052587A1 (zh) |
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EP2316283A1 (en) * | 2009-10-30 | 2011-05-04 | Nestec S.A. | Container comprising non-alcoholic compositions with visible active ingedrients |
US9803192B2 (en) | 2013-10-04 | 2017-10-31 | Cornell University | Programmable and reconfigurable microcolumn affinity chromatography device, system, and methods of use thereof |
JP6600018B2 (ja) * | 2018-01-09 | 2019-10-30 | 株式会社東芝 | 光学センサ、分析装置および分析方法 |
CN110132853B (zh) * | 2019-04-11 | 2020-06-26 | 中国科学技术大学 | 基于像素偏振相机的旋光色散测量系统及方法 |
US20230296521A1 (en) | 2022-03-17 | 2023-09-21 | Visera Technologies Company Limited | Bio-detection device, bio-detection system, and bio-detection method |
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CN1629620A (zh) * | 2003-12-19 | 2005-06-22 | 财团法人工业技术研究院 | 荧光辅助检测装置 |
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US4563331A (en) * | 1983-11-21 | 1986-01-07 | The United States Of America As Represented By The Secretary Of The Navy | System for measuring bioluminescence flash kinetics |
JP3186753B2 (ja) * | 1991-09-05 | 2001-07-11 | 株式会社日立製作所 | 核酸断片試料及び一本鎖dnaオリゴマー |
US5543018A (en) * | 1995-02-13 | 1996-08-06 | Visible Genetics Inc. | Method and apparatus for automated electrophoresis using light polarization detector |
FI954511A0 (fi) * | 1995-09-22 | 1995-09-22 | Labsystems Oy | Fluorometer |
US5736744A (en) * | 1996-03-27 | 1998-04-07 | Uvp, Inc. | Wavelength shifting filter |
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ATE363652T1 (de) * | 1997-03-07 | 2007-06-15 | Clare Chemical Res Inc | Fluorometrischer nachweis mit sichtbarem light |
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WO2000006991A2 (en) * | 1998-07-27 | 2000-02-10 | Ljl Biosystems, Inc. | Apparatus and methods for spectroscopic measurements |
GB9816088D0 (en) * | 1998-07-23 | 1998-09-23 | Axis Biochemicals Asa | System |
JP2000346802A (ja) * | 1999-03-26 | 2000-12-15 | Sony Corp | 素子内部検査装置および方法 |
JP2001133397A (ja) * | 1999-11-05 | 2001-05-18 | Fujirebio Inc | 検体の検査装置 |
DE10164058B4 (de) * | 2000-12-30 | 2008-06-12 | Krones Ag | Inspektionsvorrichtung |
JP3741051B2 (ja) * | 2001-05-10 | 2006-02-01 | 横河電機株式会社 | バイオチップ読取装置 |
DE10135766B4 (de) * | 2001-07-23 | 2006-02-16 | Nexpress Solutions Llc | Verfahren zum Erfassen eines transparenten Gegenstandes und Detektoreinrichtung |
US20030133119A1 (en) * | 2002-01-17 | 2003-07-17 | Bachur Nicholas R. | Rapid imaging of particles in a large fluid volume through flow cell imaging |
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EP1494007B8 (de) * | 2003-06-30 | 2014-09-24 | Tecan Trading AG | Vorrichtung und Verfahren zum Analysieren von Proben |
JP4113063B2 (ja) * | 2003-08-18 | 2008-07-02 | 株式会社リガク | 特定高分子結晶の検出方法 |
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JP4269079B2 (ja) * | 2004-11-01 | 2009-05-27 | 株式会社エイムテクノロジー | 散乱媒質中微量成分濃度の無侵襲測定装置 |
US20090161100A1 (en) * | 2005-11-08 | 2009-06-25 | Minot Michael J | Fiber Optic Interrogated Microslide, Microslide Kits and Uses Thereof |
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- 2006-11-03 CN CN2006800562498A patent/CN101535796B/zh not_active Expired - Fee Related
- 2006-11-03 US US12/513,293 patent/US20100067008A1/en not_active Abandoned
- 2006-11-03 EP EP06828918A patent/EP2080010A1/en not_active Withdrawn
- 2006-11-03 WO PCT/EP2006/010561 patent/WO2008052587A1/en active Application Filing
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CN1629620A (zh) * | 2003-12-19 | 2005-06-22 | 财团法人工业技术研究院 | 荧光辅助检测装置 |
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US20100067008A1 (en) | 2010-03-18 |
JP5265558B2 (ja) | 2013-08-14 |
WO2008052587A1 (en) | 2008-05-08 |
CN101535796A (zh) | 2009-09-16 |
EP2080010A1 (en) | 2009-07-22 |
JP2010508525A (ja) | 2010-03-18 |
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