CN101530466A - Nano medicament containing oregano oil and vitamins - Google Patents
Nano medicament containing oregano oil and vitamins Download PDFInfo
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- CN101530466A CN101530466A CN200910021857A CN200910021857A CN101530466A CN 101530466 A CN101530466 A CN 101530466A CN 200910021857 A CN200910021857 A CN 200910021857A CN 200910021857 A CN200910021857 A CN 200910021857A CN 101530466 A CN101530466 A CN 101530466A
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Abstract
The invention discloses a nano medicament containing oregano oil and vitamins. The grain diameter of the nano medicament is between 1 and 100 nm, and the nano medicament consists of the following compositions by mass percentage: 0.002 to 10 percent of the oregano oil, 0.00054 to 2.7 percent of vitamin A oil, 0.00008 to 0.4 percent of vitamin D3 oil, 0.001 to 5 percent of vitamin E oil, 0.00005 to 0.25 percent of vitamin K3, 0.001 to 15 percent of water-soluble vitamin, 0.001 to 5 percent of isopropyl myristate, 0.025 to 50.015 percent of surfactant, 0.003 to 15 percent of cosurfactant and 19.98 to 99.98 percent of distilled water. The medicament is mainly used for preventing and controlling alimentary bacteriosis of beasts and birds, supplementing deficiency of vitamins in vivo of animals, promoting metabolism, improving alimentation, improving feeder conversion rate, promoting growth and weight increment, and improving the immunity of organisms.
Description
Technical field
The invention belongs to field of veterinary, relate to a kind of livestock and poultry alimentary tract bacterial disease that causes by escherichia coli, Salmonella and staphylococcus aureus etc. that is used to prevent and treat, and the deficiency of replenishing vitamin in the animal body, keep animal normal physiological function, Ginkgo Biloba, promotion is searched for food, improve alimentation, improve food conversion ratio, promote growth and weightening finish, the medicine of human body immunity improving power is specifically related to the Nano medication that contains Herba Origani oil and vitamin that a kind of poultry are used.
Background technology
Herba Origani oil (Oregano oil) is a kind of volatile essential oils of extracting from the plant Adeps Bovis seu Bubali, and its main component has quinone, dipentene, coriandrol and carvacrol etc. in carvacrol, thymol, thymol, the stone.Herba Origani oil is a kind of medicated feed additive that can add use for a long time in feedstuff of China Ministry of Agriculture ([No. 2001120] are sent out in agriculture and animal husbandry) approval.Phenolic compounds such as the carvacrol in the Herba Origani oil have stronger antibacterial activity, can penetrate the cell membrane of pathogenic microorganism rapidly, change its structure, destroy its homergy function; And can stop Intramitochondrial breathing oxidizing process effectively, and make pathogenic microorganism forfeiture energy and death, have stronger inhibition and kill the function of multiple pathogenic microorganism, and can not produce drug resistance.Data shows that Herba Origani oil contains more than 30 kind of antimicrobial compound to " phytochemistry and plant species are learned " of United States Department of Agriculture (USDA), is a kind of potential natural drug growth promoter.Reports such as Sivropoulou in 1996, Herba Origani oil is to 8 kinds of G
+Bacterium has the height bacteriostasis, even still can significantly suppress the growth of bacterial strain when dilution 4000 times even 5000 times.Herba Origani oil also has malaria protozoon activity, dust Xi Shi escherichia coli, Mus Salmonella, listeria, staphylococcus aureus etc. is all had the antibacterial activity of height.Woods Tsing-Hua University in 1997 report, Herba Origani oil all has in various degree sterilization and bacteriostasis to 31 strain enteritis common bacteria, and wherein the bacteriostasis to staphylococcus aureus is the strongest, secondly is escherichia coli.Herba Origani oil has certain non-oxidizability, and it can keep greasy stable, anti-oxidation.Herba Origani oil also has malaria protozoon activity, anticoccidial and antimite activity.In addition, Herba Origani oil can improve the activity of digestive enzyme, improves food conversion ratio, and promotes growth of animal, and the enhancing body function improves specific immunity of organism specific.
Vitamin (Vitamin) is to keep healthy and the indispensable organic substance of promotion growth.Animal to its requirement seldom, usually in mg, every kind of vitamin all has its special effect, neither the energy substance of animal is not again a structural material, but the necessary participant of body substance metabolism process, belong to regulator, vitamin is the constituent of food necessity, though quantity is few, but effect is big, and irreplaceable each other.Animal is deficient in vitamin and can the generation dysbolismus causes distinctive disease, general designation subvitaminosis or deficiency disease, and several vitamin lack the disease that causes simultaneously, are called the multidimensional deficiency disease.In aquaculture, normal because the vitamin of supplying with in the animal feed is not enough or owing to digestive tract malabsorption or owing to special physiological state (gestation, suckling etc.) and reasons such as chronic or acute illness, cause various symptoms.Some vitamin is stocked in vivo, lack in a short time and can not show clinical symptoms very soon and the productivity is made a difference, continuous increase shows various symptoms gradually with the body internal consumption along with the degree that lacks increases the weight of, so in aquaculture, must in daily ration, add various vitamin, with prevention and treatment avitaminosis, and then promote to grow or breeding, enhancing immunity and anti-stress ability improve animal product output and quality, thereby increase economic benefit.
At present, in the aquaculture, the Herba Origani oil product is mainly 10% pre-mixing agent.The suitable addition of 10% Herba Origani oil pre-mixing agent is in the animal and fowl fodder per ton: piglet 130～170g, growth pig 60～120g, boar 50～80g, chicken 125～150g, middle chicken 60～100g, big chicken 60～80g, laying hen 80～100g, duckling 80～100g, meat-type duck 60～100g, aquatic feed 100～200g, rare bird 60～100g, because the Herba Origani oil dosage is very little clinically, so there are shortcomings such as spice inequality, bioavailability are low in pre-mixing agent.
Vitamin products are mainly powder multidimensional (comprise by after vitamin) and liquid vitamin (micron or millimicro meter level).Powder multidimensional (comprise by after vitamin): though bag by after the vitamin loss that causes reduced, in use spice is irregular but the powder vitamin that does not wrap quilt is faced with equally, be difficult to guarantee the mixing homogeneity of feedstuff, though the administration of can drinking water of some electrolysis multidimensional is arranged, is easy to stop up water fountain; The liquid vitamin: what at first face is how fatsoluble vitamin to be added in the aqueous solution and will to guarantee the stability of liquid.The reuse surfactant-dispersed is in water after traditional technology the inside is generally taked with fat-soluble media dissolving fatsoluble vitamin.But after joining water the inside, often become turbid, emulsus or vaporific, even oil phase and liquid phase separation, oil bloom float on the upper strata, cause the uniformity to descend, and stop up water fountain, and can cause water fountain to grow a large amount of antibacterials after the fat-soluble increase.Tradition vitamin products use cost height: seem low price, but addition is big, bioavailability is very low, and the average bioavailability of fatsoluble vitamin is about 30% only in traditional vitamin products, and major part is not absorbed by body.
Summary of the invention
At the shortcomings and deficiencies of above-mentioned prior art, the object of the present invention is to provide a kind of be suitable for clinical practice contain Herba Origani oil and vitamin Nano medication.This medicine is mainly used in the livestock and poultry alimentary tract bacterial disease that control is caused by escherichia coli, Salmonella and staphylococcus aureus etc., and the deficiency of replenishing vitamin in the animal body, keep animal normal physiological function, Ginkgo Biloba, promotion is searched for food, and improves alimentation, improves food conversion ratio, promote growth and weightening finish, human body immunity improving power.
Realize that foregoing invention purpose technical scheme is the Nano medication that contains Herba Origani oil and vitamin that a kind of poultry are used, particle diameter is between 1～100nm, and its raw material and mass percent thereof are:
Its raw material and mass percent thereof are:
Herba Origani oil 0.002%～10% vitamin A oil 0.00054%～2.7%
Vitamin D
3Oil 0.00008%～0.4% vitamin E oil 0.001%～5%
Vitamin K
30.00005%～0.25% water soluble vitamins 0.001%～15%
Isopropyl myristate 0.001%～5% surfactant 0.025%～50.015%
Cosurfactant 0.003%～15% distilled water 19.98%～99.90%
The total weight percent of above-mentioned raw materials is 100%;
Described water soluble vitamins is thiamine, riboflavin, pyridoxol, vitamin B
12, one or more the mixture in the calcium pantothenate, nicotiamide, biotin;
Described surfactant is any one in polyoxyethylene ether (40) castor oil hydrogenated (RH40), polyoxyethylene ether (40) Oleum Ricini (EL40) and the tween 80;
Described cosurfactant is any one in ethanol, propylene glycol, the 1,3 butylene glycol.
Above-mentioned raw materials is pharmaceutical grade, food stage or feed grade raw material;
The liquid-drop diameter size of Nano medication of the present invention is between 1～100nm.
Medicine of the present invention is mainly used in the livestock and poultry alimentary tract bacterial disease that control is caused by escherichia coli, Salmonella and staphylococcus aureus etc., and the deficiency of replenishing vitamin in the animal body, keep animal normal physiological function, Ginkgo Biloba, promotion is searched for food, and improves alimentation, improves food conversion ratio, promote growth and weightening finish, human body immunity improving power.
The present invention specifically dilutes 2000～5000 times with medicine of the present invention with drinking water mainly by oral administration, freely drinks logotype in every month 3～10 days for animal.
Innovation part of the present invention is by nanotechnology Herba Origani oil and multivitamin to be carried out compound, has made the Nano medication that contains Herba Origani oil and vitamin that a kind of poultry are used.Problems such as this medicine has solved the problem of Herba Origani oil pre-mixing agent spice inequality, and the easy oxidation of vitamin products, and the molten water capacity is prone to muddiness, causes the uniformity to descend, and stops up water fountain, and bioavailability is low.Concrete property of the present invention: preparation method is simple, stable storage, and thin up water/oil phase does not separate; Liquid droplet size is little, but for the transparency liquid infinite dilution, is evenly distributed in drinking-water, can guarantee that animal is taken in evenly in the colony; Good hydrophilic property, be uniformly dispersed, stability is high, and the absorption rate height discharges in gastrointestinal rapidly, contact rapidly with intestinal wall, can be by upright tapping between epithelial cell into blood circulation, the average bioavailability of mill run is about 30%, and the present invention can reach 99%, also can absorb well even suffer from the animal of hepatic disease and fat malabsorption, individual utilization variance reduces; Diffusibility is good, can be uniformly dispersed moment in the cold water, and microgranule is near hydrone, and is extremely strong with the bonding force of water, can produce high dissolving diffusibility; Relatively, the nano-emulsion dilute with water still is a transparency liquid with conventional emulsification product (micron or millimicro meter level), but infinite dilution is a stabilising system, the bioavailability height, and dosage reduces, and toxic and side effects reduces or eliminates, and is easy to use, and safety is efficient; Nanotechnology makes between Herba Origani oil and the vitamin stable each other and does not disturb mutually, and interface technology makes Herba Origani oil and vitamin be difficult for oxidation and volatilization, has improved stability of drug greatly.
Description of drawings
Fig. 1 is the electromicroscopic photograph figure of Nano medication of the present invention.
Fig. 2 is the particle size distribution figure of Nano medication of the present invention.
The specific embodiment
Below further set forth the beneficial effect of medicine of the present invention by testing example, these are tested examples and have comprised the medicine stability test of the present invention and the clinical test of pesticide effectiveness.
Test example 1 stability experiment
1, test material
Product with preparation among the embodiment 1 is a sample.
2, test method
(1) centrifugal acceleration test
In centrifuge tube in, put in high speed centrifuge in right amount by sealing orifice for sample thief, with the centrifugal 20min of 10000rpm.Observe outward appearance of the present invention and whether change, and at the bottom of centrifuge tube, have or not drug precipitation.
(2) temperature stability test
Sample is sub-packed in several vials, places under 4 ℃, 25 ℃, the 37 ℃ conditions lucifuge to place after the sealing 3 months, every 10d sampling is observed.Observe outward appearance of the present invention and whether change, measure particle size distribution.
(3) long-term stable experiment
Sample is sub-packed in several vials, places under the room temperature lucifuge to place after the sealing to keep sample and investigate 1 year, sampling in per 3 months is observed.Observe outward appearance of the present invention and whether change, measure particle size distribution.
3, result of the test
Sample is centrifugal stability test result show: through the centrifugal 20min of 10000rpm, outward appearance is still transparent, does not see physical changes such as layering, flocculation, and not seeing at the bottom of centrifuge tube has drug precipitation.
Sample temperature stability test result shows: placed 3 months under 4 ℃, 25 ℃, 37 ℃ conditions, outward appearance is still transparent, does not see physical changes such as layering, flocculation, and particle size distribution is in 1～100nm.
Sample long-term stable experiment result shows: placed 1 year down in room temperature, outward appearance is still transparent, does not see physical changes such as layering, flocculation, and particle size distribution is in 1～100nm.
The test of pesticide effectiveness in test example 2 bodies
1, test material
Be subjected to the reagent thing: sample, the 10% Herba Origani oil pre-mixing agent of preparation in the embodiment of the invention 1.
Animal subject: 600 10 age in days healthy AA broiler are selected in test for use, and test group (sample among the embodiment 1) is divided into high, medium and low 3 dosage groups, each 100; 100 of medicine matched groups (10% Herba Origani oil pre-mixing agent); 100 of positive controls (infection) without medicine; 100 of negative control group (not infecting) without medicine.
2, test method
Choose the sick chicken that is diagnosed as Pullorum Disease, gather pathological material of diseases such as the heart, liver, spleen, directly line agar plate and separate, cultivate 24h for 37 ℃, selected suspicious bacterium colony, be transplanted on the bouillon agar slant medium, cultivate (37 ℃) 24h, identify, be defined as the Pullorum Disease Salmonella by microscopy, biochemical reaction, Salmonella diagnostic serum, choose typical strain then and cultivate (37 ℃) 24h with ordinary broth, the concentration that records bacterium liquid with the Maxwell turbidimetry is 6 * 10
12Individual/L, with it as the counteracting toxic substances vaccine.
Test group, medicine matched group, positive controls chickling are all by 0.8mL/ subcutaneous injection; Negative control group adopts 0.8mL/ subcutaneous injection of sterilization ordinary broth.Occur the symptom identical in the 24h, choose 5 positive controls and cut open inspection and laboratory diagnosis, thereby prove that this challenge test is successful with white scour of chicken salmonellosis.After the chickling morbidity, press table 1 administration, successive administration 5 days, experimental period are 10 days, regularly observe every day and respectively organize the searching for food of chicken, drinking-water, mental status, clinical symptoms; The situation that record is dead, and the chicken that dies of illness is carried out necropsy separate with antibacterial.
Table 1 test chicken grouping administration situation
Curative effect determinate standard
Cure: after the medication, the clinical symptoms complete obiteration; Search for food, drink water and physical signs such as spirit recovers normal fully.
Effectively: after the medication, clinical symptoms partly disappears; Search for food, drink water and physical signs such as spirit recovers normal substantially.
Invalid: after the medication, clinical symptoms do not see and alleviates, even worsens or dead.
3, result of the test
In 10 days that test is carried out, physical signs such as the mortality rate of each group, spirit recover normal substantially.Cure rate and the relative weight gain rate etc. see Table 2.As can be seen from the results, the effective percentage of high, medium and low 3 dosage groups of the present invention and and cure rate all than the height of 10% Herba Origani oil pre-mixing agent.
Table 2 medicine of the present invention is to the curative effect of the white scour of chicken
3 clinical trials of test example
1, test material
Be subjected to the reagent thing: sample, 10% Herba Origani oil pre-mixing agent, the commercially available electrolysis multidimensional of preparation in the embodiment of the invention 1.
Animal subject: 800 1 age in days healthy AA broiler are selected in test for use, test I group (sample among the embodiment 1) 200, test II group (10% Herba Origani oil pre-mixing agent) 200, test III group (commercially available electrolysis multidimensional) 200,200 of blank groups.
2, test method
Test group I group is drunk 2000 times of diluents of sample in the embodiment of the invention 1 every day, test II group, feed every day and contain the feedstuff of 10% Herba Origani oil pre-mixing agent, addition is that every kilogram of feed adds 100mg, test III group, drink electrolysis multidimensional aqueous solution every day, the blank group is without medicine.Identical daily ration (basal diet prescription and trophic level see Table 3), to test under similar raising production environment, management condition, the immune programme for children unanimity, experimental period is 42d, promptly 1 breeding cycle is online flat foster, writes down feed consumption rate, death toll every day.
Test chicken adopts online flat supporting, and 0～3 age in week of early stage broods in the sealing hen house of temperature control, and test chicken is raised by certain scale of feeding, freely drinks water, and the employing natural lighting combines with artificial lighting, carries out immunity according to the immune programme for children of broiler.
Test is divided into two stages, and 1-3 age in week is a stage, and 4-6 age in week is a stage, respectively chicken is weighed at the whole story in two stages respectively, counts the feed consumption of respectively organizing chicken and the death rate of every day, calculates daily gain and feedstuff-meat ratio.
3, result of the test
Each is organized feed consumption, daily gain, the feedstuff-meat ratio of chicken and reaches death rate, the results are shown in Table 4.As can be seen from the results, the present invention all is better than 10% Herba Origani oil and commercially available electrolysis multidimensional to the influence of chicken fertility performance, and feedstuff-meat ratio of the present invention is 2, and daily gain is 46.1, and death rate is 5%.
Table 3 basal diet prescription and trophic level
Table 4 medicine of the present invention is to the influence of meat chicken production performance
Embodiment 1
1) preparation method of Herba Origani oil nano-emulsion: at normal temperatures and pressures, after 5g Herba Origani oil, 2.5g isopropyl myristate, 25g tween 80, the mixing of 7.5g ethanol, stir, slowly to wherein adding the 10g distilled water, the limit edged stirs, and forms the Herba Origani oil nano-emulsion;
2) preparation method of vitamin nanoemulsion: at normal temperatures and pressures, with 0.54g vitamin A oil, 0.08g vitamin D
3After oil, 1g vitamin E oil and 4.86g tween 80 mixed, homogenizing 30min slowly contained the 0.05g vitamin K to wherein dripping then
3, 0.18g thiamine, 0.72g riboflavin, 0.3g pyridoxol, 0.0009g vitamin B
12, 1g calcium pantothenate, 2.7g nicotiamide, 0.015g biotin distilled water 38.55g, the limit edged stirs, the system viscosity is less during beginning, along with the adding of water, it is sticky that system can become, and continues to drip and constantly stir, after the amount of water reaches certain value, system again can be thinning, then water is added to q.s, promptly forms vitamin nanoemulsion;
3) with after Herba Origani oil nano-emulsion and the vitamin nanoemulsion mixing, stir, promptly.
With the present invention with 2000 times of drinking water dilutions after, the poultry such as chicken, duck, Carnis Coturnicis japonicae of can feeding, both can be used for preventing and treating the fowl digestive tract bacterial disease that causes by escherichia coli, Salmonella and staphylococcus aureus etc., can replenish the deficiency of fatsoluble vitamin in the animal body again, keep animal normal physiological function, Ginkgo Biloba, promotion is searched for food, improve alimentation, improve food conversion ratio, promote growth and weightening finish.
Embodiment 2
1) preparation method of Herba Origani oil nano-emulsion: at normal temperatures and pressures, after 5g Herba Origani oil, 2.5g isopropyl myristate, 25g tween 80,7.5g mixed with propylene glycol, stir, slowly to wherein adding the 10g distilled water, the limit edged stirs, and forms the Herba Origani oil nano-emulsion;
2) preparation method of vitamin nanoemulsion: at normal temperatures and pressures, with 0.54g vitamin A oil, 0.08g vitamin D
3After oil, 1g vitamin E oil and 4.86g tween 80 mixed, homogenizing 30min slowly contained the 0.05g vitamin K to wherein dripping then
3, 0.18g thiamine, 0.72g riboflavin, 0.3g pyridoxol, 0.0009g vitamin B
12, 1g calcium pantothenate, 2.7g nicotiamide, 0.015g biotin distilled water 38.55g, the limit edged stirs, the system viscosity is less during beginning, along with the adding of water, it is sticky that system can become, and continues to drip and constantly stir, after the amount of water reaches certain value, system again can be thinning, then water is added to q.s, promptly forms vitamin nanoemulsion;
3) with after Herba Origani oil nano-emulsion and the vitamin nanoemulsion mixing, stir, promptly.
With the present invention with 2000 times of drinking water dilutions after, the poultry such as chicken, duck, Carnis Coturnicis japonicae of can feeding, both can be used for preventing and treating the fowl digestive tract bacterial disease that causes by escherichia coli, Salmonella and staphylococcus aureus etc., can replenish the deficiency of fatsoluble vitamin in the animal body again, keep animal normal physiological function, Ginkgo Biloba, promotion is searched for food, improve alimentation, improve food conversion ratio, promote growth and weightening finish.
Embodiment 3
1) preparation method of Herba Origani oil nano-emulsion: at normal temperatures and pressures, after 5g Herba Origani oil, 2.5g isopropyl myristate, 25g tween 80, the mixing of 7.5g ethanol, stir, slowly to wherein adding the 10g distilled water, the limit edged stirs, and forms the Herba Origani oil nano-emulsion;
2) preparation method of vitamin nanoemulsion: at normal temperatures and pressures, with 1.35g vitamin A oil, 0.2g vitamin D
3After oil, 2.5g vitamin E oil and 12.15g tween 80 mixed, homogenizing 30min slowly contained the 0.125g vitamin K to wherein dripping then
3Distilled water 33.675g, the limit edged stirs, the system viscosity is less during beginning, along with the adding of water, it is sticky that system can become, and continues to drip and constantly stir, after the amount of water reaches certain value, system again can be thinning, then water is added to q.s, promptly gets and form vitamin nanoemulsion;
3) with after Herba Origani oil nano-emulsion and the vitamin nanoemulsion mixing, stir, promptly get the present invention.
With the present invention with 5000 times of drinking water dilutions after, the ruminants such as cattle and sheep of can feeding, both can improve the digestive enzyme activity of animals such as cattle and sheep, improve food conversion ratio, promote to search for food, promote growth and weightening finish, can replenish the deficiency of fatsoluble vitamin in the animal body again, keep animal normal physiological function, improve rate of fertilization, accelerate rehabilitation after being ill etc.
Embodiment 4
1) preparation method of Herba Origani oil nano-emulsion: at normal temperatures and pressures, after 5g Herba Origani oil, 2.5g isopropyl myristate, 25g polyoxyethylene ether (40) Oleum Ricini (EL40), 7.5g mixed with propylene glycol, stir, slowly to wherein adding the 10g distilled water, the limit edged stirs, and forms the Herba Origani oil nano-emulsion;
2) preparation method of vitamin nanoemulsion: at normal temperatures and pressures, with 0.54g vitamin A oil, 0.08g vitamin D
3After oil, 1g vitamin E oil and 4.86g polyoxyethylene ether (40) Oleum Ricini (EL40) mixed, homogenizing 30min slowly contained the 0.05g vitamin K to wherein dripping then
3, 0.18g thiamine, 0.72g riboflavin, 0.3g pyridoxol, 0.0009g vitamin B
12, 1g calcium pantothenate, 2.7g nicotiamide, 0.015g biotin distilled water 38.55g, the limit edged stirs, the system viscosity is less during beginning, along with the adding of water, it is sticky that system can become, and continues to drip and constantly stir, after the amount of water reaches certain value, system again can be thinning, then water is added to q.s, promptly forms vitamin nanoemulsion;
3) with after Herba Origani oil nano-emulsion and the vitamin nanoemulsion mixing, stir, promptly.
With the present invention with 2000 times of drinking water dilutions after, the poultry such as chicken, duck, Carnis Coturnicis japonicae of can feeding both can be used for preventing and treating the fowl digestive tract bacterial disease that is caused by escherichia coli, Salmonella and staphylococcus aureus etc., can replenish vitamin deficiency in the animal body again, keep animal normal physiological function, Ginkgo Biloba promotes to search for food, and improves alimentation, improve food conversion ratio, promote growth and weightening finish, improve rate of fertilization, accelerate rehabilitation after being ill etc.
Embodiment 5
1) preparation method of Herba Origani oil nano-emulsion: at normal temperatures and pressures, after 10g Herba Origani oil, 5g isopropyl myristate, 50g tween 80, the mixing of 15g ethanol, stir, slowly to wherein adding the 19.83g distilled water, the limit edged stirs, and forms the Herba Origani oil nano-emulsion;
2) preparation method of vitamin nanoemulsion: at normal temperatures and pressures, with 0.00054g vitamin A oil, 0.00008g vitamin D
3After oil, 0.001g vitamin E oil and 0.015g tween 80 mixed, homogenizing 30min slowly contained the 0.00005g vitamin K to wherein dripping then
3, distilled water 0.15g, the limit edged stirs, the system viscosity is less during beginning, along with the adding of water, it is sticky that system can become, and continues to drip and constantly stir, after the amount of water reaches certain value, system again can be thinning, then water is added to q.s, promptly forms vitamin nanoemulsion;
3) with after Herba Origani oil nano-emulsion and the vitamin nanoemulsion mixing, stir, promptly.
With the present invention with 5000 times of drinking water dilutions after, the ruminants such as cattle and sheep of can feeding, both can improve the digestive enzyme activity of animals such as cattle and sheep, improve food conversion ratio, promote to search for food, promote growth and weightening finish, can replenish the deficiency of fatsoluble vitamin in the animal body again, keep animal normal physiological function, improve rate of fertilization, accelerate rehabilitation after being ill etc.
Embodiment 6
1) preparation method of Herba Origani oil nano-emulsion: at normal temperatures and pressures, after 0.002g Herba Origani oil, 0.001g isopropyl myristate, 0.012g polyoxyethylene ether (40) Oleum Ricini (EL40), the mixing of 0.003g ethanol, stir, slowly to wherein adding the 0.004g distilled water, the limit edged stirs, and forms the Herba Origani oil nano-emulsion;
2) preparation method of vitamin nanoemulsion: at normal temperatures and pressures, with 2.7g vitamin A oil, 0.4g vitamin D
3After oil, 5g vitamin E oil and 24.3g polyoxyethylene ether Oleum Ricini (EL40) mixed, homogenizing 30min slowly contained the 0.025g vitamin K to wherein dripping then
3, 0.45g thiamine, 1.8g riboflavin, 0.75g pyridoxol, 0.0023g vitamin B
12, 2.5g calcium pantothenate, 6.75g nicotiamide, 0.038g biotin distilled water 55.28g, the limit edged stirs, the system viscosity is less during beginning, along with the adding of water, it is sticky that system can become, and continues to drip and constantly stir, after the amount of water reaches certain value, system again can be thinning, then water is added to q.s, promptly forms vitamin nanoemulsion;
3) with after Herba Origani oil nano-emulsion and the vitamin nanoemulsion mixing, stir, promptly.
With the present invention with 5000 times of drinking water dilutions after, the animals such as pig, chicken of can feeding both can have been improved animal disease resistant power, can replenish vitamin deficiency in the animal body again, keep animal normal physiological function, Ginkgo Biloba promotes to search for food, and improves alimentation, improve food conversion ratio, promote growth and weightening finish, improve rate of fertilization, accelerate rehabilitation after being ill etc.
Embodiment 7
1) preparation method of Herba Origani oil nano-emulsion: at normal temperatures and pressures, after 0.002g Herba Origani oil, 0.001g isopropyl myristate, 0.012g polyoxyethylene ether (40) Oleum Ricini (EL40), the mixing of 0.003g ethanol, stir, slowly to wherein adding the 0.004g distilled water, the limit edged stirs, and forms the Herba Origani oil nano-emulsion;
2) preparation method of vitamin nanoemulsion: at normal temperatures and pressures, with 0.00054g vitamin A oil, 0.00008g vitamin D
3After oil, 0.001g vitamin E oil and 0.015g polyoxyethylene ether Oleum Ricini (EL40) mixed, homogenizing 30min slowly contained the 0.00005g vitamin K to wherein dripping then
3Distilled water 99.97g, the limit edged stirs, the system viscosity is less during beginning, along with the adding of water, it is sticky that system can become, and continues to drip and constantly stir, after the amount of water reaches certain value, system again can be thinning, then water is added to q.s, promptly forms vitamin nanoemulsion;
3) with after Herba Origani oil nano-emulsion and the vitamin nanoemulsion mixing, stir, promptly.
The present invention is directly fed animals such as cattle, sheep, both can improve animal disease resistant power, can replenish the deficiency of fatsoluble vitamin in the ruminant body again, keep animal normal physiological function, Ginkgo Biloba, promotion is searched for food, improve alimentation, improve food conversion ratio, promote growth and weightening finish, improve rate of fertilization, accelerate rehabilitation after being ill etc.
Embodiment 8
1) preparation method of Herba Origani oil nano-emulsion: at normal temperatures and pressures, after 5g Herba Origani oil, 2.5g isopropyl myristate, 25g polyoxyethylene ether (40) castor oil hydrogenated (RH40), the mixing of 7.5g ethanol, stir, slowly to wherein adding the 10g distilled water, the limit edged stirs, and forms the Herba Origani oil nano-emulsion;
2) preparation method of vitamin nanoemulsion: at normal temperatures and pressures, with 0.54g vitamin A oil, 0.08g vitamin D
3After oil, 1g vitamin E oil and 6.48g polyoxyethylene ether castor oil hydrogenated (RH40) mixed, homogenizing 30min slowly contained the 0.05g vitamin K to wherein dripping then
3, 0.18g thiamine, 0.72g riboflavin, 0.3g pyridoxol, 0.0009g vitamin B
12, 1g calcium pantothenate, 2.7g nicotiamide, 0.015g biotin distilled water 36.93g, the limit edged stirs, the system viscosity is less during beginning, along with the adding of water, it is sticky that system can become, and continues to drip and constantly stir, after the amount of water reaches certain value, system again can be thinning, then water is added to q.s, promptly forms vitamin nanoemulsion;
3) with after Herba Origani oil nano-emulsion and the vitamin nanoemulsion mixing, stir, promptly.
With the present invention with 5000 times of drinking water dilutions after, the poultry such as chicken, duck, Carnis Coturnicis japonicae of can feeding both can be used for preventing and treating the fowl digestive tract bacterial disease that is caused by escherichia coli, Salmonella and staphylococcus aureus etc., can replenish the deficiency of vitamin in the animal body again, keep animal normal physiological function, Ginkgo Biloba promotes to search for food, and improves alimentation, improve food conversion ratio, promote growth and weightening finish, improve rate of fertilization, accelerate rehabilitation after being ill etc.
Embodiment 9
1) preparation method of Herba Origani oil nano-emulsion: at normal temperatures and pressures, with 5g Herba Origani oil, 2.5g isopropyl myristate, 25g polyoxyethylene ether (40) castor oil hydrogenated (RH40), 7.5g 1, after the 3-butanediol mixes, stir, slowly to wherein adding the 10g distilled water, the limit edged stirs, and forms the Herba Origani oil nano-emulsion;
2) preparation method of vitamin nanoemulsion: at normal temperatures and pressures, with 0.54g vitamin A oil, 0.08g vitamin D
3After oil, 1g vitamin E oil and 6.48g polyoxyethylene ether castor oil hydrogenated (RH40) mixed, homogenizing 30min slowly contained the 0.05g vitamin K to wherein dripping then
3, 0.18g thiamine, 0.72g riboflavin, 0.3g pyridoxol, 0.0009g vitamin B
12, 1g calcium pantothenate, 2.7g nicotiamide, 0.015g biotin distilled water 36.93g, the limit edged stirs, the system viscosity is less during beginning, along with the adding of water, it is sticky that system can become, and continues to drip and constantly stir, after the amount of water reaches certain value, system again can be thinning, then water is added to q.s, promptly forms vitamin nanoemulsion;
3) with after Herba Origani oil nano-emulsion and the vitamin nanoemulsion mixing, stir, promptly.
With the present invention with 5000 times of drinking water dilutions after, the poultry such as chicken, duck, Carnis Coturnicis japonicae of can feeding both can be used for preventing and treating the fowl digestive tract bacterial disease that is caused by escherichia coli, Salmonella and staphylococcus aureus etc., can replenish vitamin deficiency in the animal body again, keep animal normal physiological function, Ginkgo Biloba promotes to search for food, and improves alimentation, improve food conversion ratio, promote growth and weightening finish, improve rate of fertilization, accelerate rehabilitation after being ill etc.
Claims (2)
1, a kind of Nano medication that contains Herba Origani oil and vitamin, the particle diameter that it is characterized in that this nano-emulsion preparation is between 1～100nm, and its raw material and mass percent thereof are:
Herba Origani oil 0.002%～10% vitamin A oil 0.00054%～2.7%
Vitamin D
3Oil 0.00008%～0.4% vitamin E oil 0.001%～5%
Vitamin K
30.00005%～0.25% water soluble vitamins 0.001%～15%
Isopropyl myristate 0.001%～5% surfactant 0.025%～50.015%
Cosurfactant 0.003%～15% distilled water 19.98%～99.90%
The total weight percent of above-mentioned raw materials is 100%;
Described water soluble vitamins is thiamine, riboflavin, pyridoxol, vitamin B
12, one or more the mixture in the calcium pantothenate, nicotiamide, biotin;
Described surfactant is any one in polyoxyethylene ether castor oil hydrogenated, polyoxyethylene ether Oleum Ricini and the tween 80;
Described cosurfactant is any one in ethanol, propylene glycol, the 1,3 butylene glycol.
2. the application of the described Nano medication of claim 1 in treatment livestock and poultry alimentary tract bacterial disease.
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