CN104922163B - Brood health care bag and its application method of a kind of green cultivation chicken - Google Patents
Brood health care bag and its application method of a kind of green cultivation chicken Download PDFInfo
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Abstract
The invention discloses brood health care bag and its application methods of a kind of green cultivation chicken.The health care bag of brooding of green cultivation chicken of the present invention is made of injection part and drinking-water part, wherein, injection part is formed by several antibiotic combinations；Drinking-water part is formed by combining by glucose, compound probiotic and multi-vitamins.Injection part in health care bag of the present invention of brooding have many advantages, such as it is rapid-action, small to animal injury, can quickly kill the in vivo vertical transmission bacterial disease of chick；The glucose of drinking-water part can supplement rapidly baby chick physical efficiency, compound probiotic can occupy-place chick rapidly enteron aisle, advantage digestion flora is established by breeding, effectively improves the utilization rate of feed；Multi-vitamins can provide all kinds of trace elements needed for chick growth.The present invention brood health care bag have many advantages, such as high-efficient purification vertical transmission disease, chick is damaged it is small, reduce feedstuff-meat ratio, reduce place harmful bacteria vicious circle, easy for operation, the ability to resist stress of chick and the nutrition that growth is required are taken into account simultaneously, are suitble to promote and apply in aquaculture.
Description
Technical field
The present invention relates to animal healths and field of veterinary, and in particular to a kind of green cultivation chicken brood health care bag and its
Application method.
Technical background
Green cultivation is the best outlet of aquaculture.With the expanding economy of China, people have gone up the rich date excessively,
Material life enriches degree continuous improvement, and requirement of the people to the food securities such as meat, eggs and milk, taste flavor also gradually steps up, more next
More people favors high-quality safety, the green agricultural product of pure flavor.As the food-safe attention of country and the world are moved
The appealing of object welfare, country's, animal-breeding environment residual to medicine will be more stringent, and green cultivation is undoubtedly poultry husbandry after us
Best outlet.This undoubtedly requires poultry farming process strictly through green cultivation theory, except strengthening feeding management, chicken all living creatures's state
Beyond putting in a suitable place to breed, the critical period in feeding process：The care plans of brood time are also required to adjust to adapt to green cultivation development
Demand.But for a long time, Traditional health care medicine there are antibiotic usage mode not science, purification vertical transmission disease effects it is poor,
It destroys intestinal health digestion flora, be also easy to produce drug resistance, the shortcomings of packaging operation is inconvenient.There are some companies positive at present
Exploitation green is brooded health care medicinal, but exist the probiotic active time that purification disease effects are poor, product is unstable, are added it is short,
The problem of operating with inconvenience, therefore the health care bag of efficiently brooding for developing a breeder green cultivation has become urgently to be resolved hurrily
Problem.
Tradition is brooded, and also there are many defects for health care medicinal, it is impossible to meet extensive green cultivation demand.
First, science, purification vertical transmission disease effects are not poor, right for the antibiotic usage mode in traditional health care medicinal of brooding
Infant animal damage is big.Shown through investigation, in the market 95% brood health care medicinal based on drinking water type antibiotic, inhaled by enteron aisle
It is poor to whole body profile vertical transmission disease bactericidal effect after animal body metabolic adsorption after receipts based on sterilization, cause blood
The not high body area of concentration still residual harmful bacterium.It is larger to animal organ's damage in drug metabolism processes, easily kidney is caused to swell
And hepatic disease, so as to influence the growth of chick.
Secondly, the antibiotic in Traditional health care medicine, by intestinal absorption, destroys intestinal health digestion bacterium based on drinking water type
Group, causes indigestion, diarrhea etc..Before birth, healthy animal is in germ-free condition in parent.Infant animal is mainly from parent
Intestinal flora is obtained with external environment, is born 2 days, animal gastrointestinal tract is colonized completely by microorganism, is finally settled down in gastrointestinal tract
Flora can be stabilized about 1014 microorganisms, be planted up to more than 500.Host, normal flora and external environment form a microecosystem
System.Drinking water type antibiotic also kills substantial amounts of profitable strain in enteron aisle during being sterilized to infant animal, makes just lively
The health digestion flora imbalance of object, harmful bacteria take advantage of the occasion to invade occupy-place enteron aisle site, and dominant microflora substitutes, and flora ratio is lost
It adjusts, this is that young age livestock and poultry indigestion, diarrhea, feedstuff-meat ratio increase one of the main reasons.
Again, the not scientifical use of antibiotic easily causes place harmful bacteria vicious circle, drug resistance in traditional health care medicinal of brooding.
Drinking water type antibiotic also kills substantial amounts of profitable strain in enteron aisle, makes infant animal during being sterilized to infant animal
Health digestion flora imbalance, harmful bacteria takes advantage of the occasion invasion occupy-place enteron aisle site, and after an infectious cycle, disease is quick-fried again
Hair, foster family, which has to reuse antibiotic, kills pernicious bacteria, is thus absorbed in the vicious circle of abuse of antibiotics, makes
The drug resistance of harmful bacteria constantly enhances or even can turn out similar India in place and Pakistani " super drug resistance is thin
Bacterium " finally pasts medical help.
Tradition is brooded health care medicinal component packaging, inconvenient for operation, and cost is caused in large-scale farming to increase.The existing guarantor that broods
Strong medicine is unpackaged complete, and it is indefinite to feed medicine suit mark daily, and resisting stress medicine, energy supplement medicine etc. are also added when feeding,
In large-scale farming production, aging, low productivity poultry feeders increase, and in order to ensure medication in place, technical staff is first
It needs to consider compatibility of drugs, secondly need to emphasize usage again to poultry feeders, waste many energy and material resources, recessiveness adds
Aquaculture cost.
Finally, tradition health care medicinal of brooding using antibiotic is leading, does not add environmentally friendly active bacteria formulation actively, it is impossible to full
The extensive green cultivation demand of foot.Green cultivation is a kind of environmentally friendly aquaculture model, is total to biological in breeding environment
Raw complementarity principle, makes various microorganisms counteract each other, reduces the use of antibiotic, do not destroy original breeding environment to the full extent
The ecological balance.The animal products of green cultivation gets consumer reception again due to its quality is high, in good taste, residual without hormone drug, product
Supply falls short of demand.Antibiotic usage can only kill the bacterium sensitive to its, and beneficial bacterium is also largely killed while killing harmful bacteria,
The harmful bacteria for finally resulting in drug resistance is occupied an leading position, and is deteriorated environmental ecology circle, is not advocated by green cultivation.
The content of the invention
It is an object of the invention to overcome existing disadvantages mentioned above existing for health care medicinal of brooding, providing one kind can high-efficient purification
Vertical transmission disease damages chick small, reduction feedstuff-meat ratio, reduces the vicious circle of farming site harmful bacteria, operate with method
The health care bag of brooding of easy green cultivation chicken.
Another object of the present invention is the application method for the health care bag of brooding for providing above-mentioned green cultivation chicken.
Above-mentioned purpose of the present invention is achieved by the following technical programs：
The following green cultivation chickens of the present invention brood health care bag so that every part can be for 2000 chick uses as an example, not
Represent that the present invention only has a kind of this selection.In actually cultivating, it can be carried out according to actual conditions according to proportioning provided by the invention
It prepares.
The health care bag of brooding of a kind of green cultivation chicken, including injection part and drinking-water part；
Wherein, the injection part for tinfoil paper it is packed, by 12 ~ 16g of injection stage Cefradine, injection stage amikacin 12 ~
16g and 500 ~ 700 ml of sterile saline compositions；
The drinking-water part by forming as follows：（1）Labeled as the Fresco Bag of " opening is used ", include：1.0×1010CFU/g's
20 ~ 40g of bacillus subtilis, 1.0 × 101010 ~ 20g of plant lactobacillus of CFU/g, 1.0 × 1010The S. cervisiae of CFU/g
200 ~ 260g of 10 ~ 20g and glucose；
（2）Labeled as the Fresco Bag of " 1 age in days ", 1.0 × 10 are included1020 ~ 40g of bacillus subtilis of CFU/g, 1.0 ×
101010 ~ 20g of plant lactobacillus of CFU/g, 1.0 × 101010 ~ 20g of S. cervisiae of CFU/g, coating multi-vitamins 40 ~
60g and 150 ~ 200g of sodium acid carbonate compositions；
（3）Labeled as the Fresco Bag of " 2 age in days ", 1.0 × 10 are included1020 ~ 40g of bacillus subtilis of CFU/g, 1.0 ×
101010 ~ 20g of plant lactobacillus of CFU/g, 1.0 × 101010 ~ 20g of S. cervisiae of CFU/g, coating multi-vitamins 40 ~
60g and 150 ~ 200g of sodium acid carbonate compositions；
（4）Labeled as the Fresco Bag of " 3 age in days ", 1.0 × 10 are included1020 ~ 40g of bacillus subtilis of CFU/g, 1.0 ×
101010 ~ 20g of plant lactobacillus of CFU/g, 1.0 × 101010 ~ 20g of S. cervisiae of CFU/g, coating multi-vitamins 40 ~
60g and 150 ~ 200g of sodium acid carbonate compositions.
As a kind of optimal technical scheme, in above-mentioned health care bag of brooding, the injection part is packed for tinfoil paper, by injection stage
Cefradine 14g, injection stage amikacin 14g and sterile saline 600ml compositions；
The drinking-water part by forming as follows：（1）Labeled as the Fresco Bag of " opening is used ", include：1.0×1010CFU/g's
Bacillus subtilis 30g, 1.0 × 1010The plant lactobacillus 15g of CFU/g, 1.0 × 1010The S. cervisiae 15g of CFU/g and
Glucose 240g；
（2）Labeled as the Fresco Bag of " 1 age in days ", 1.0 × 10 are included10The bacillus subtilis 30g of CFU/g, 1.0 ×
1010The plant lactobacillus 15g of CFU/g, 1.0 × 1010S. cervisiae 15g, coating multi-vitamins 48g and the carbonic acid of CFU/g
Hydrogen sodium 192g is formed；
（3）Labeled as the Fresco Bag of " 2 age in days ", 1.0 × 10 are included10The bacillus subtilis 30g of CFU/g, 1.0 ×
1010The plant lactobacillus 15g of CFU/g, 1.0 × 1010S. cervisiae 15g, coating multi-vitamins 48g and the carbonic acid of CFU/g
Hydrogen sodium 192g is formed；
（4）Labeled as the Fresco Bag of " 3 age in days ", 1.0 × 10 are included10The bacillus subtilis 30g of CFU/g, 1.0 ×
1010The plant lactobacillus 15g of CFU/g, 1.0 × 1010S. cervisiae 15g, coating multi-vitamins 48g and the carbonic acid of CFU/g
Hydrogen sodium 192g is formed.
The application method of the health care bag of brooding of green cultivation chicken of the present invention is in the following order：
Sterile saline in the injection part is sufficiently mixed with injection stage Cefradine and injection stage amikacin
Afterwards, enter in chick in 8 ~ 12h of farming site and inject, control water 0.5 ~ 1h of material before injecting, injection dosage is 0.3ml/；
The drinking-water part（1）Application method be to be dissolved with 30 jin of clean waters, for injecting the injection portion
The chick divided is drunk；
The drinking-water part（2）Application method be in drinking portion（1）It, will with 30 jin of clean waters after drinking
Drinking-water part（2）Dissolving, drinks for the chick of 1 age in days；
The drinking-water part（3）Application method be to be dissolved with 40 jin of clean waters, drunk for the chick of 2 ages in days；
The drinking-water part（4）Application method be to be dissolved with 40 jin of clean waters, drunk for the chick of 3 ages in days.
In above-mentioned component proportioning, each component is that commercially available universal feed is preferred with raw material, because of the raw material of each producer
Quality difference is larger, and the variation of numerical value is on the fundamental property of product without influence, but efficiently probiotics and coating are multiple for part of drinking water
It is improper to close vitamin selection, product packaging flatulence can be made to swell, product probiotics effective active declines soon, and expected effect is not achieved
Fruit.
Compared with prior art, the present invention has the advantages that：
（Ⅰ）The present invention enters in chick will be in the health care bag of brooding of the green cultivation chicken in 8 ~ 12h of farming site
Injection partial syringe in Chickens, the in vivo vertical transmission bacterium of chick can be killed in 4h, makes it that can not pollute foster
Grow place；
（Ⅱ）The health care bag of brooding of green cultivation chicken of the present invention select Cefradine and amikacin to chick into
Row injection, both medicines cooperation there is synergy, blood concentration can be made to be rapidly reached peak value, with it is quick, to young bird
The advantages of chicken damage is small；
（Ⅲ）In the health care bag of brooding of green cultivation chicken of the present invention, part of drinking water（1）Glucose can be fast
Speed supplement chick physical efficiency, it is emergent to alleviate transport；Compound probiotic can occupy-place chick in a short time enteron aisle, breeding establishes advantage and disappears
Change flora, improve efficiency of feed utilization；
（Ⅳ）In the health care bag of brooding of green cultivation chicken of the present invention, the compound probiotic for part of drinking water is with resistance to height
Warm, acidproof, resistance to choline, activity be strong, moderate for reference factor, combining with green cultivation demand, most at last bacillus subtilis,
The probiotics that plant lactobacillus and S. cervisiae are combined；Because bacillus subtilis is nontoxic, in gastric juice after being taken
It is middle to bring back to life and develop rapidly, then in large intestine, caecum mucous membrane surface hyperplasia, it can inhibit the harmful bacteria and spoilage organisms grape in enteron aisle
Intestinal bacilli illness is corrected in coccus, the field planting and intrusion of Pseudomonas aeruginosa, Escherichia coli, shigella dysenteriae, and metabolite is fourth
Acid is intestinal epithelial tissue cytothesis and the Major Nutrient substance repaired；Plant lactobacillus is life important in animal gastrointestinal tract
One of obligate anaerobic flora is managed, it is nontoxic, harmless, animal health can be promoted；Compared with other lactic acid bacterias, acidproof and bile tolerance energy
Power is stronger, and can survive under the adverse circumstances of nutrition unevenness, so into human body intestinal canal survival rate higher, colonization ability is more
By force；Plant lactobacillus is added when infant animal intestinal microecology system is begun setting up, can customize and play occupy-place in enteric epithelium
Enough lactic acid is acted on and generated, gut pH is reduced, effectively inhibits the growth of pathogenic bacteria and spoilage organisms, promotes enterocinesia, dimension
Hold intestinal microecology balance and normal physiological digestion；S. cervisiae is activity and stronger one kind of metabolic function among microorganism
Amphimicrobe is a kind of natural feed raw material, can improve animal feed digestibility and palatability, and metabolite is more
Up to more than 100 kinds, wherein what is be relatively well known is aromatic substance, multi-vitamins, oligosaccharides, organic acid, peptide etc., moved in young age
Using the feed containing S. cervisiae on object, animal health condition is can obviously improve, improves feed digestibility；The present invention is educating
Feed above-mentioned several probiotics young early period, can effectively maintain intestinal health, avoid widely applying antibiotic to animal for a long time and
The health of the mankind brings harm, in addition can establish intestinal health flora, aid digestion, reduces feedstuff-meat ratio；
（Ⅴ）In the health care bag of brooding of green cultivation chicken of the present invention, part of drinking water（2）~（4）Multi-vitamins
It is good with stability, the effect of all kinds of trace elements needed for chick growth can be effectively provided；
（Ⅵ）In the health care bag of brooding of green cultivation chicken of the present invention, part of drinking water（2）~（4）Sodium acid carbonate tool
There is stomach invigorating, adjust kidney lithate, detumescence；
（Ⅶ）The health care bag of brooding of green cultivation chicken of the present invention can establish the enteron aisle digestion of health after use
Interior environment reduces harmful bacteria and infects occupy-place enteron aisle, so as to reduce the bowel problems occurred in chick growth；With Traditional health care medicine
It compares, the usage amount of antibiotic significantly reduces, and cost is also reduced while reducing the damage to chick.
Specific embodiment
With reference to embodiment, the present invention is further elaborated.But embodiment is not the present invention any type of
It limits.
Embodiment 1
The health care bag of brooding of a kind of green cultivation chicken, including injection part and drinking-water part；
Wherein, the injection part is packed for tinfoil paper, by injection stage Cefradine 14g, injection stage amikacin 14g and nothing
Bacterium physiological saline 600ml is formed；
The drinking-water part by forming as follows：（1）Labeled as the Fresco Bag of " opening is used ", include：1.0×1010CFU/g's
Bacillus subtilis 30g, 1.0 × 1010The plant lactobacillus 15g of CFU/g, 1.0 × 1010The S. cervisiae 15g of CFU/g and
Glucose 240g；
（2）Labeled as the Fresco Bag of " 1 age in days ", 1.0 × 10 are included10The bacillus subtilis 30g of CFU/g, 1.0 ×
1010The plant lactobacillus 15g of CFU/g, 1.0 × 1010S. cervisiae 15g, coating multi-vitamins 48g and the carbonic acid of CFU/g
Hydrogen sodium 192g is formed；
（3）Labeled as the Fresco Bag of " 2 age in days ", 1.0 × 10 are included10The bacillus subtilis 30g of CFU/g, 1.0 ×
1010The plant lactobacillus 15g of CFU/g, 1.0 × 1010S. cervisiae 15g, coating multi-vitamins 48g and the carbonic acid of CFU/g
Hydrogen sodium 192g is formed；
（4）Labeled as the Fresco Bag of " 3 age in days ", 1.0 × 10 are included10The bacillus subtilis 30g of CFU/g, 1.0 ×
1010The plant lactobacillus 15g of CFU/g, 1.0 × 1010S. cervisiae 15g, coating multi-vitamins 48g and the carbonic acid of CFU/g
Hydrogen sodium 192g is formed.
Packing method：In humidity less than 40% RH, the make-up room of 25 DEG C of temperature, good required ratio raw material are weighed, are packed into
Stainless steel V-type mix powder machine mixing half an hour, after by the semi-finished product mixed with semi-automatic powder can packing machine dispense, sealing.
1 health care bag of the present invention of experimental example and clean-up effect, chick Influence on kidney of the Traditional health care medicine to white diarrhea
First, materials and methods
1.1 test material
Traditional health care medicine 1:Bar is produced only by the honest animal pharmaceutical estate in Henan, mark composition colistin sulfate, Ofloxacin, a left side
Revolve imidazoles etc.；Traditional health care medicine 2:It broods precious by the production of Hebei speed wing science and technology medicine company, mark composition Ofloxacin, aloe extraction
Object, astragalus polyose, taurine, multivitamin etc.；Traditional health care medicine 3:Strong man is produced by the strong medicine company of Guangdong Hengfeng, is marked into
It is divided into grand sight, woods Mycosporin.
Present invention group B groups are 1 gained health care bag of embodiment.
Experimental animal：Second generation Qingyuan City fiber crops cock, 2000 plumage is provided by seed farm, there is higher source of parents dysentery characterized by white mucous stool carrying rate.
Diagnostic reagent：White diarrhea fowl typhoid multivalence dyeing plate agglutination test antigen is limited purchased from marine growth science and technology in Beijing
Company.
1.2 experimental design and feeding management
Test site is handled：It is cleaned through form garbage and dejection, caustic soda spray disinfectant 3 times, stringent empty house one month, playground shakedown
The programs such as quick lime are spread, guarantee is provided without endogenous white diarrhea pathogeny for space for activities.
Grouping and feeding management：Second generation of experimental animal Qingyuan City fiber crops cock, 2000 plumage is equally divided into 5 groups, every group immediately
A 4 repeat, each to repeat 100 plumages, in the scientific base raising of Tian Nong companies, feeding management according to《The Guangdong day limited public affairs of agriculture food
Take charge of broiler production administrative standard and operation manual 2013 editions》Perform, basal diet according to《Qingyuan City of Guangdong Tiannong Food Co., Ltd.
Numb chicken feeding program 20140810 editions》It performs.
The experiment grouping of table 1
1.3 assay method
1.3.1 tablet agglutination：The whole chickens each repeated are taken a blood sample about respectively under 7 ages in days, 14 age in days wings
0.15mL is centrifuged out serum, and drawing 50uL antigens with liquid-transfering gun, vertically drop on clean glass plate, takes serum 50uL and antigen
It is uniformly mixed, and applies the liquid level for dissipating into diameter about 2.0cm, and constantly rock glass plate, judged in 2 minutes as a result, 100% aggegation note
It records as " +++ ", is determined as strong positive serum；50% aggegation is recorded as " ++ ", is determined as weakly positive；It is not aggregated and does not keep a record, judge
For feminine gender；Record calculates every percentage.
1.3.2 dissect observation kidney swells situation：10 dissects are extracted immediately in 7 ages in days, each repetition of every group of 14 ages in days, are seen
Examine kidney enlargement situation, record calculates kidney and swells rate.
1.4 data statistics
One-way analysis of variance carries out each processing group data using 17.0 softwares of spss, LSD methods carry out Multiple range test,
As a result use（Mean+SD）It represents.
2nd, result and analysis
The influence that 2.1 different health care medicinals swell to the dysentery characterized by white mucous stool clean-up effect and kidney of 7 age in days chickens
By table 2 as it can be seen that compared to Traditional health care medicine, dysentery characterized by white mucous stool clean-up effect is apparent in 7 age in days of health care bag of the present invention, to chick without
Kidney swells damage.7 age in days dysentery characterized by white mucous stool Serum Antibody Detections show that this morbidity group B strong positives rate, weakly positive rate are 0.00%, weakly positive
Rate 0.00%, pole are substantially less than control group A 6.25%（P<0.01）, bar organize C 2.00% only（P<0.01）, strong man organize E 4.25
（P<0.01）；The kidney rate of swelling of 7 ages in days compares display, this morbidity group B swells without kidney, and kidney swells rate 0.00%, and pole is substantially less than Traditional health care
Medicine group（Bar organizes C 42.50%, the precious group D 22.50% that broods, strong man's group E 60.00% only）（P<0.01）.
The influence that the different health care medicinals of table 2 swell to the dysentery characterized by white mucous stool clean-up effect and kidney of 7 age in days chickens
Note：The identical person of same column shoulder note letter represents that difference is not notable（P>0.05）, lowercase difference person represent difference show
It writes（P<0.05）, capitalization difference person represent difference it is extremely notable（P<0.01）, similarly hereinafter.
The influence that 2.2 different health care medicinals swell to the dysentery characterized by white mucous stool clean-up effect and kidney of 14 age in days chickens
By table 3 as it can be seen that compared to Traditional health care medicine, health care bag of the present invention still has strong dysentery characterized by white mucous stool clean-up effect in 14 ages in days, can
It is fast to infer the dysentery characterized by white mucous stool pathogeny that it is killed entrained by chick so that source of parents dysentery characterized by white mucous stool pathogeny minimizes the pollution level of environment.14
Age in days dysentery characterized by white mucous stool Serum Antibody Detection shows that this morbidity group B strong positives rate 0.00%, pole is substantially less than control group A 10.00%（P<
0.01）, bar organize C 3.61% only（P<0.01）, brood precious group D 4.72%（P<0.01）, strong man organize E 5.22（P<0.01）；This
Invention group B weakly positives rate 5.56%, pole, which is substantially less than, compares A 31.94%（P<0.01）, bar organize C 14.44% only（P<0.01）, educate
Young precious group D 17.22%（P<0.01）, strong man organize E 25.26（P<0.01）.
The influence that the different health care medicinals of table 3 swell to the dysentery characterized by white mucous stool clean-up effect and kidney of 14 age in days chickens
2 stability test of experimental example
First, prescription and testing index are implemented
1.1 implement prescription
1.1.1 it is 1 gained health care bag of embodiment to implement prescription.
1.1.2 according to implement prescription production 400 sets of finished product, by finished product in ordinary room temperature, be protected from light lower preservation, monthly extract 5
Sample packet is measured it, METHOD FOR CONTINUOUS DETERMINATION 12 months.
1.2 testing index
1.2.1 product characteristics measure
Monthly 2 times（15 days/time）It checks packed products character, and records.Observation index：Whether there is flatulence, finished product Fresco Bag
Interior pulverulent mixture whether there is caking, whether there is color change.
1.2. room temperature preserves efficient probiotics survival rate in lower product and measures
Survival rate=number of viable（Bacillus subtilis+Bacillus acidi lactici+S. cervisiae）/ producer ensures content sum.It is living
Bacterium number amount（CFU/g bacterium powders）=clump count × 2 × dilution factor, because being with the addition of other components during health care package dress, etc.
Be same as having carried out efficient probiotics 5 times of dilutions, thus it is final ensure content be scaled containing bacillus subtilis >=0.1 ×
1010CFU/ grams, Bacillus acidi lactici >=0.5 × 109CFU/ grams, S. cervisiae >=0.5 × 109CFU/ grams.
Number of viable measures：Sample takes 10g, with the mortar grinder to sterilize, sample is made to homogenize.1g is taken to homogenize
Sample is dissolved in 9mL sterile waters, is uniformly mixed.1mL suspensions is taken to be added to the test tube equipped with 9mL sterile waters, mixing is equal
It is even.1mL is taken from the bacterium solution of just mixing, is added in the test tube that another fills 9mL sterile waters.Repetitive operation, to bacterium solution
Carry out continuous 10 times of dilutions.The bacterium solution of three suitable gradients is chosen, 0.5mL is taken, is applied on tablet, every piece of tablet applies 3
A continuous dilution gradient, each 3 repetitions of gradient.It after the tablet that painting wipes slightly is dried, is inverted, is put into 37 DEG C of biochemical perseverances
In warm incubator.It takes out tablet afterwards for 24 hours, chooses dilution factor numeration of the colony count between 30~300, and calculate
Viable count in product.
2nd, result and analysis
2.1 product characteristics measurement results
This implementation prescription health care package side has good stability, in December it is even without packaging flatulence, without caking, powder sample appearance is without change
Change.
In the lower different time of 2.2 room temperature storage in product efficiently probiotics determination of activity as a result, referring to table 4.
By table 4 as it can be seen that health care bag prescription of the present invention preserves December at normal temperatures, the efficient probiotics survival rate in product
Average >=95.0% has higher stability, can reach product design demand.
4 room temperature of table stores the survival rate of efficient probiotics in product in lower different time（%）
Note：Repeating sample number n=5；"/" represents no added.
2.2 analysis
It can be with the efficient probiotics prescription in prescription this experiment proved that being preferably coated with compound vitamin in prescription（Withered grass
Bacillus, plant lactobacillus, S. cervisiae）Between without biochemical reaction, it is not no the adverse reactions such as swollen bag, caking occur, and efficiently
Probiotic active is lasting, stablizes, and meets design requirement.
Influence of the 3 health care bag of the present invention of experimental example to meat chicken production performance
First, materials and methods
1.1 test material
Implementation prescription is 1 gained health care bag of embodiment.
Experimental animal：22000 plumage of Qingyuan City's fiber crops hen seedling provided by hatching factory of company.
1.2 experimental designs and feeding management
1 age in days Qingyuan City fiber crops hen of Yunling base, 22000 plumage, random point 4 groups are put down foster, every group of 5 repetitions（With different footnotes,
Same shelf rearing is put in support）, it is each to repeat 1100 plumages.For control group A without using any health care medicinal, test group is divided into implementation group B, single needle agent
Group C, single drinking-water group D, perform according to different prescriptions（It is shown in Table 5）.Feeding management according to《Guangdong Tiannong Food Co., Ltd.'s broiler chicken
Production management standard and operation manual 2013 editions》Perform, basal diet according to《Guangdong Tiannong Food Co., Ltd.'s Qingyuan Chicken is fed
Expect program 20140810 editions》It performs.
The experiment grouping of table 5
1.3 testing index
Number is washed in a pan with dead per day entry feed intake, the 14th age in days of test chicken randomly selects 50 to each repetition in each group
It weighs on an empty stomach.Calculate death and culling rate, the uniformity and feedstuff-meat ratio, statistical table making.Test chicken health status is observed, it 3 days before brood time will
Butt-swelling, the chick of not material feeding are eliminated, and check dead chick, record dead wash in a pan.
1.4 statistical analysis
One-way analysis of variance carries out each processing group data using 17.0 softwares of spss, LSD methods carry out Multiple range test,
As a result use（Mean+SD）It represents.
2nd, result and analysis
Influence of the 2.1 different prescriptions to the performance of broiler production
In terms of chick death and culling rate is reduced, by table 3 as it can be seen that implementation group B poles substantially reduce the death and culling rate of chick（0.68%）.
Compare control group A（1.57%）Pole substantially reduces 0.89 percentage point（P<0.01）, than single needle agent group C（1.09%）Pole substantially reduces
0.41 percentage point（P<0.01）, than single drinking-water group D（1.22%）Pole substantially reduces 0.54 percentage point（P<0.01）.Illustrate reality
A group B is applied, i.e., using injection part and drinking-water part effect of drugs can be made to reach maximization simultaneously.
6 14 Day-old Broiler Chickens production performance of table
Note：The identical person of same column shoulder note letter represents that difference is not notable（P>0.05）, lowercase difference person represent difference show
It writes（P<0.05）, capitalization difference person represent difference it is extremely notable（P<0.01）.
Increasing uniformity aspect, by table 6 as it can be seen that implementation group B（Value 62.00）It is significantly higher than other each groups, compares control group A
（Value 54.60）Pole dramatically increases 7.4（P<0.01）, than single needle agent group C（Value 59.40）Pole dramatically increases 2.6（P<0.01）, than list
Drinking-water group D（Value 57.00）Pole dramatically increases 5（P<0.01）.Illustrate implementation group B, i.e., simultaneously using injection part and drinking-water part
Effect of drugs can be made to reach maximization, dramatically increase the uniformity of chick.Injection group C and control group A significant difference（P<0.05）,
And list drinking-water group D and control group difference is not notable（P>0.05）, it is very big to illustrate that injection part is played in raising uniformity aspect
Effect.
In terms of daily ingestion amount and weight, by table 6 as it can be seen that implementation group B daily ingestion amounts（Value 13.34g）It is significantly higher than control
0.81 gram of group A (value 12.53g), weight (value 105g) are significantly higher than 2 grams of control group A (value 103g)（P<0.05）, single needle agent group
C, list drinking-water group D and control group A difference is not notable（P>0.05）.Illustrate only while using injection part and drinking-water part ability
It is optimal to increase daily ingestion amount and weight.
In terms of feedstuff-meat ratio is reduced, by table 6 as it can be seen that implementation group B（Value 2.21）Pole is substantially less than control group A（Value 2.31）
0.1 point（P<0.01）, pole be substantially less than single needle agent group C（Value 2.30）0.09 point（P<0.01）, single drinking-water group D（Value 2.22）
And control group A（Value 2.31）, single needle agent group C（Value 2.30）Difference is extremely notable, illustrates the drinking-water part in prescription, i.e. addition is efficient
Probiotics can significantly improve efficiency of feed utilization, reduce bowel problems, reduce feedstuff-meat ratio.
In conclusion compared with other each implementation groups, the prescription in implementation group B is preferred plan, both injection part and drink
Water section simultaneously using the production performance that can integrate before improving 14 ages in days, reduce it is dead wash in a pan, the raising uniformity, increase daily ingestion amount,
It reduces bowel problems, reduce feedstuff-meat ratio.
Claims (1)
1. the health care bag of brooding of a kind of green cultivation chicken, it is characterised in that including injection part and drinking-water part；
Wherein, the injection part is packed for tinfoil paper, by injection stage Cefradine 14g, injection stage amikacin 14g and sterile life
Manage brine 600ml compositions；
The drinking-water part by forming as follows：（1）Labeled as the Fresco Bag of " opening is used ", include：1.0×1010The withered grass of CFU/g
Bacillus 30g, 1.0 × 1010The plant lactobacillus 15g of CFU/g, 1.0 × 1010The S. cervisiae 15g and grape of CFU/g
Sugared 240g；
（2）Labeled as the Fresco Bag of " 1 age in days ", 1.0 × 10 are included10The bacillus subtilis 30g of CFU/g, 1.0 × 1010CFU/g
Plant lactobacillus 15g, 1.0 × 1010S. cervisiae 15g, the coating multi-vitamins 48g and sodium acid carbonate 192g of CFU/g
Composition；
（3）Labeled as the Fresco Bag of " 2 age in days ", 1.0 × 10 are included10The bacillus subtilis 30g of CFU/g, 1.0 × 1010CFU/g
Plant lactobacillus 15g, 1.0 × 1010S. cervisiae 15g, the coating multi-vitamins 48g and sodium acid carbonate 192g of CFU/g
Composition；
（4）Labeled as the Fresco Bag of " 3 age in days ", 1.0 × 10 are included10The bacillus subtilis 30g of CFU/g, 1.0 × 1010CFU/g
Plant lactobacillus 15g, 1.0 × 1010S. cervisiae 15g, the coating multi-vitamins 48g and sodium acid carbonate 192g of CFU/g
Composition.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101978852A (en) * | 2010-09-06 | 2011-02-23 | 成都和谐生物科技有限公司 | Microbial feed additive for treating piglet yellow and white dysentery and preparation method thereof |
| CN102626385A (en) * | 2012-04-26 | 2012-08-08 | 福建森宝食品集团股份有限公司 | Method for adding antibiotics into poultry oil emulsion inactivated vaccine |
| CN103621469A (en) * | 2013-11-28 | 2014-03-12 | 广州动物园 | Artificial incubation and artificial brooding method for pelicans |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101978852A (en) * | 2010-09-06 | 2011-02-23 | 成都和谐生物科技有限公司 | Microbial feed additive for treating piglet yellow and white dysentery and preparation method thereof |
| CN102626385A (en) * | 2012-04-26 | 2012-08-08 | 福建森宝食品集团股份有限公司 | Method for adding antibiotics into poultry oil emulsion inactivated vaccine |
| CN103621469A (en) * | 2013-11-28 | 2014-03-12 | 广州动物园 | Artificial incubation and artificial brooding method for pelicans |
Non-Patent Citations (2)
| Title |
|---|
| 微生态制剂预防鸡白痢沙门氏菌感染的应用技术研究;薛俊龙等;《中国动物检疫》;20111231;第28卷(第9期);第38-41页 * |
| 肉鸡饲养过程中应注意的几个问题;王家乡等;《养殖与饲料》;20071231(第12期);第8-12页 * |
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