KR20010068226A - composition containing chitosan oligo saccharide and aminoethyl sulfonic acid for use as fodder and drinking water additives - Google Patents

Abstract

PURPOSE: Provided is a nutrient composition containing chitosan oligosaccharide and Taurine that is added to livestock feed or a kind of drink, which is effective for promoting growth of livestock and building up immunity and disease resistance. CONSTITUTION: The composition includes 3-60g/L of chitosan oligosaccharide and 10-300g/L of aminoethyl sulfonic acid as a main ingredient and also contains kinds of vitamins such as vitamin A, D3, E, B1, B2, B6, B12, C, K, nicotinic acid, folic acid, calcium pantothenate and choline chloride, amino acid and inorganic matters. 1-4 liter or the composition is added to 1 ton of livestock or drink.

Description

Composition containing chitosan oligo saccharide and aminoethyl sulfonic acid for use as fodder and drinking water additives}

The present invention relates to a nutrient composition which is added to animal feed and drinking water for the purpose of promoting livestock growth, improving feed efficiency, and enhancing the history of disease, and more specifically, feed and drinking water containing chitosan oligosaccharides and taurine as main ingredients. It relates to a nutrient composition for addition.

Animal feed and drinking water of chickens, pigs and cows are generally added with feed and negative nutrients for the purpose of promoting livestock growth and improving feed efficiency. And probiotics containing dry cells have been mainly used.

The probiotics generally include Saccharomyces cervisiae, Aspergillus oryzae, Bacillus thuringiensis, Lactobacillus acidophilus, and Rhodobacter capsules. Rhodobacter capsulatus, Lactobacillus sp., Streptomyces sp., Bacillus sp., Etc., contain the strain as dry cells. It is used for the purpose of increasing feed efficiency, reducing stress and early recovery, adjuvant treatment of various diseases, improving utilization rate and palatability of various nutrients by improving intestinal function.

The vitamin agent is different in the composition of each product, but usually vitamin A, vitamin B ₂ , vitamin B ₆ , vitamin B ₁₂ , various vitamins such as calcium pantothenate, niacin, vitamin C, vitamin D, vitamin E, vitamin K, magnesium, sulfate It contains minerals such as copper, iron, cobalt and iodine, and it is added to animal feed and drinking water for the purpose of promoting the growth of animals, improving the feed efficiency, increasing the anti-potent, prevention of abnormal milk and prevention of the disease. Has been administered.

Previously, vitamins were mainly used as feed and negative nutritional supplements, but recently, the use of probiotics has increased, and vitamins and probiotics are similar in terms of overall amount of use, and their purpose and effects are similar.

Further, my patent application No. 98-41584 said, "The feed contains chitosan oligosaccharides 500-5,000mg / kg and vitamins necessary for animals as main ingredients, and it is mixed and administered by 2-4kg per ton of animal feed. The present invention relates to a "nutrient additive composition containing chitosan oligosaccharides", which replaces conventional feed additives such as weight gain and improved feed efficiency by organic synergism of chitosan oligosaccharides and vitamins. .

The present invention has a new chitosan oligosaccharide and taurine containing no side effects and safer than the conventional feed and negative nutritional supplements described above, in terms of promoting the growth of the livestock, improving the weight gain, and improving the feed efficiency. It is an object of the present invention to provide a nutritional composition for feed and drinking water.

In order to achieve the above object, the present invention is the addition of chitosan oligosaccharides and taurine in addition to the general vitamins used as feed and negative nutritional supplements, as well as the useful effects of vitamins, as well as the antimicrobial action, immune enhancing action, anti-potentiation action of chitosan oligosaccharides, etc. Osmotic pressure regulation, antioxidant, detoxification, membrane stabilization, neuromodulation, optic nerve protection, cardiac and neuronal development of the taurine are synergistically synergistic with each other to promote animal growth, feed efficiency and weight gain It is characterized in that it shows an excellent effect, such as enhancement, enhancement of immunity, anti-medical history.

1 is a comparison curve of the number of deaths of each animal.

Hereinafter, the present invention will be described in more detail.

Chitosan oligosaccharides, which are the main components of the present invention, chitosan oligosaccharides and taurine-containing feeds and nutritional additives for negative additives, are partially decomposed products of chitin and chitosan, and are highly functional bioactive substances that exhibit high water solubility while maintaining the physiological properties of chitosan. to be. Chitin and chitosan are naturally occurring polysaccharides and are recently attracting special attention. Chitin (β-poly-Ν-acetyl-D-glucosamine) is a shell of crustaceans such as crabs. It is a kind of nitrogen-containing polysaccharide extracted from the nature, but is widely used in nature, but its use is very limited because it is insoluble in water-soluble solvents. In comparison, chitosan is a natural polymer made by deacetylating chitin (β-poly-D-glucosamine), that is, a natural polymer polysaccharide in which glucosamine is bound to β-1,4, and the chitin is heated in sodium hydroxide. It is obtained by the deacetylation treatment of, and has been widely used because it has a wide range of physiological activities and exhibits water solubility in weak acidity. However, even though it shows water solubility in weak acidity, chitosan is generally hardly soluble and highly viscous, so it is difficult to apply to food because it changes the physical properties of food, and it is not easily absorbed by the human digestive tract even when ingested due to its low solubility in water and digestive fluids. It is difficult to expect the effect compared to the excellent functionality.

Chitosan Oligo Saccharide solves these problems of chitin and chitosan, and it shows high solubility in water-soluble solvents while maintaining the physiological properties of chitosan, so it is easy to add to food and has excellent body absorption rate. It is attracting the attention of the food and pharmaceutical industry as a functional bioactive substance. Chitosan oligosaccharides have the structure of chitosan, and two to ten glucosamine hexasaccharides (monosaccharides) are bound and have the following chemical structure.

(an integer from 0 to 8)

Since chitosan oligosaccharides maintain the physiological properties of chitosan, they enhance immune function, act on cellular activity, lower blood pressure, regulate cholesterol, promote excretion of carcinogens, release radioactive substances, and improve anemia and hepatic function. It has been reported to exhibit a variety of physiological activities, such as intestinal and digestive action, uric acid release promoting action, antibacterial action, anti-cancer and anti-tumor action, calcium absorption promotion action, blood sugar rise suppression effect.

The content of chitosan oligosaccharides contained in the present invention chitosan oligosaccharides and taurine-containing feed and a negative additive nutritional composition can be adjusted according to the nutritional status, health status, feed of the administered animal. The more chitosan oligosaccharides are contained, the stronger the effects of the above-mentioned chitosan oligosaccharides are, so the efficacy becomes stronger, but considering the economics of the feed for animals and drinking water, about 3 to 60 g per 1 l of the composition solution is a preferred range of use. .

Taurine, which is the main ingredient of the present invention chitosan oligosaccharides and taurine-containing feed and negative additive nutritional composition, is another kind of aminoethyl sulfonic acid, which is a kind of essential amino acid present in large quantities in stray animals. , Mainly in brain cells or nerve cells, heart tissues, eye tissues, muscle tissues, liver tissues, etc. are present at a high concentration of 10mM or more, and in these organs, neuromodulation, cell membrane stabilization, detoxification, antioxidant activity, osmotic pressure regulation, Ca ²⁺ is a component that plays a variety of physiological roles of the cells ranging from transport regulation, optic neuroprotective, cardiac and neuronal development. As a result, it has been found to be excellent for adult diseases such as atherosclerosis, hypertension, stroke, brain cell protection, heart failure, vision enhancement, and cataract prevention.

Taurine is a β-amino acid, unlike other amino acids, and has an amino group bonded to β-carbon, and has a structure of sulfonic acid (-SO ₃ H) instead of carboxylic acid. Taurine is biosynthesized primarily by the liver from sulfur-containing amino acids such as methionine and cysteine in the diet or in the diet. In mammals, taurine is no longer metabolized as the final metabolite of sulfur-containing amino acids.

The content of taurine contained in the feed containing the chitosan oligosaccharide and taurine of the present invention and the nutrient composition for negative addition may be adjusted according to the nutritional state, health condition, feed, etc. of the administered animal. The more taurine is contained, the stronger the effects of the taurine-specific effects described above, but the efficacy becomes stronger, but considering the economical efficiency of animal feed and drinking water, about 10 to 300 g per 1 l of the composition solution is a preferred range of use.

Vitamins are essential for the normal metabolism of all cells in animals, and most vitamins are required in very small amounts in the body. However, not all vitamins needed for animal growth need to be supplied as feed because some vitamins are synthesized and supplied in the animal's body. For example, ruminants can supply the amount required for metabolism in the body because microorganisms living in the rumen synthesize vitamin B group, and in addition, vitamin C can be synthesized and supplied by many animals in the body. It is known that there is no need to supply. Therefore, vitamins developed as feed and negative nutritional supplements are usually formulated around the vitamins required for the administered animal and aim to contain sufficient amounts that do not interfere with growth while preventing deficiency.

Therefore, the composition of the present invention mainly aims to be used in chickens, pigs, cows, etc., and includes the vitamins necessary for the growth of these animals in an amount sufficient for growth, the composition of the specific vitamins included in the composition of the present invention and The content is appropriately adjusted within the administration range of the general vitamin agent according to the administration animal and administration conditions. In addition, the composition of the present invention may further include nutritional ingredients such as amino acids and minerals in addition to vitamins.

The composition according to the present invention is an organic synergistic effect of chitosan oligosaccharides and taurine and vitamins excellent growth promoting effect, growth rate improvement effect, immunity strengthening effect, anti-potentiation effect and feed efficiency improvement effect that were not found in conventional feed and negative nutritional supplements And the like.

The present composition is made of a yellow to red yellow liquid according to the preparation of a general liquid formulation based on chitosan oligosaccharide and taurine. The specific composition of the vitamin can be adjusted according to the administration animal and administration conditions, sterile purified water can be added in an appropriate amount.

In addition, the composition may further include other nutritional ingredients that are acceptable for animals.

The composition is mixed and administered to animal feed and drinking water, such as chicken, pigs, cattle, etc., the dosage can be adjusted according to the nutritional state, health condition, feed of the administered animal, usually about 1 to 4ℓ per ton of drinking water It is preferred to administer.

The present invention will be described in more detail with reference to the following Examples. However, the scope of the present invention is not limited by the following examples, and ordinary changes by those skilled in the art are possible within the scope of the technical idea of the present invention.

Example 1

Preparation of Feed and Negative Nutritional Compositions Containing Chitosan Oligosaccharides and Taurine

According to the manufacturing method of the pharmacovi vi revised liquid formulation was made a nutritional composition for feed addition according to the present invention in the composition shown in Table 1.

Furtherance amount Vitamin B ₁ 2,000 mg Vitamin B ₂ 1,000 mg Vitamin B ₆ 1,000 mg Vitamin B ₁₂ 1,000 mg Vitamin c 1,000 mg Aminoethyl sulfonic acid 10,000 mg Chitosan oligosaccharides 3,000 mg Sterile purified water Quantity

Example 2

Measurement of weight change after administration to finishing pigs

Exam conditions

The composition liquid of the present invention was mixed by 1 L per ton of feed and negative water and administered until shipment, and the shipment date and delivery weight and mortality were investigated and compared by dividing the experimental group into 447 heads and 258 heads.

Shipping date birthday Head Day of Shipment (Days) Total breeding days (day) Total weight (Kg) Average weight (Kg) Remarks 99.2.9 98.7.13 7 211 1,477 824 103 7.26 One 202 202 2.10 7.26 4 203 812 429 107.3 2.12 7.26 11 205 2,255 1,100 100 3.3 7.13 One 233 233 104 104 3.19 7.13 2 249 498 543 108.6 9.26 One 174 174 10.1 2 169 338 3.20 10.1 One 170 170 92 92 3.23 7.13 One 253 253 94 94 3.26 9.26 2 181 362 1,373 105.6 10.1 11 176 1,936 3.29 7.13 3 259 777 1,510 100.7 9.26 12 184 2,208 total 248 181.2 44,947 26,274 105.9

Experiment head Shipping head Average Shipment Age (Days) Average Shipping Weight (Kg) Death toll (mortality rate) Remarks Control 258 248 181.2 105.9 10 (3.88) Experimental group 447 441 176.4 108.7 6 (1.34) Difference 4.8 2.8 4 (2.54)

As can be seen from the table as described above, when the composition of the present invention was administered until the pigs are shipped, the mean delivery age was 176.4 days for the experimental group and 181.2 days for the control group, which was shortened by 4.8 days for the experimental group. The 108.7kg, 105.8kg of the control group was further increased by 2.8kg in the experimental group, 6 heads (1.34) in the lung head count experimental group, 10 heads (3.88) in the control group the composition of the present invention shortens the delivery date of the hog pig, It can be seen that it increases the effect and significantly lowers mortality.

In addition, when the composition of the present invention is administered to pigs until shipment, when one head consumes 250 kg of feed, the consumption of the composition of the present invention is 250 ml, costing 1,750 won, and increasing weight of 2.8 kg. have. When looking at the increase effect only, as shown in Table 4 below, when the price of 1kg living body is 625 won, the price of the drug and the increase is the same, which is a breakeven point.

Raw / kg 625 1,000 1,500 1,600 1,700 1,800 1,900 2,000 2,100 2,200 2,300 Profit (won / two) 0 1,050 2,450 2,730 3,010 3,290 3,570 3,850 4,130 4,410 4,690

Furthermore, when the composition of the present invention was administered to piglets until shipment, the delivery date was reduced by 4.8 days and the feed was 19.2 kg when eating 4 kg per day. If the composition of the present invention is administered to pigs until shipment, there is an increase in income of 10,540 won per head (100kg 210,000 won) due to weight gain and feed reduction, and the income will be further increased considering the reduction in labor costs due to the reduction of shipping age. .

Example 3

Effect on Feed Efficiency, Weight Gain and Anti-Histesis During Broiler

Exam conditions

Experimental Animal: Broiler

Number of experiments: 40,000 (distributed to 4 dongs of 10,000 animals each)

Experimental preparation: ① Building A: 60 kg (2 kg / ton) of nutritional supplement composition for feed containing chitosan oligosaccharides of the Korean Patent Application No. 98-41584 (hereinafter referred to as "chitovita")

② B: 30 kg (1 kg / ton) of chitosan preparation (hereinafter referred to as "chitonal acid")

③ C: 30L (1 L / ton) of the composition of the present invention (hereinafter referred to as "chitovitam solution")

④ Building D: Vitamin Preparation (hereinafter referred to as "Vita Chorus") 30L (1 L / ton)

Experimental Method: Each product per thousand chickens was added ①, ② to feed and ③, ④ to negative water. Visits were made 2-3 times a week to observe weight gain, disease infection, mortality and fecal odor. After the shipment, the total feed consumption, the total weight of the shipment and the cost of production were calculated to confirm the effect on the productivity and economics of the chitosan preparation. In addition, all the experimental groups were added with plasma plasma (Eastern seedlings) for 3 days after the start of the injection, and enloxyl (Dongwon New Drug) for 3 days after the 8-day vaccine.

Comparison table of dead shooter A (chitonal acid) B (from Chitovita) C (quitovitav) D (Vita Chorus) 1 day old 2 25 30 38 24 3 27 26 26 18 4 8 4 15 9 5 9 8 12 15 6 20 18 8 11 7 10 6 9 8 8 7 7 12 7 9 8 9 20 12 10 8 3 12 5 11 5 31 (cat) 15 9 12 11 13 17 15 13 13 13 12 14 14 5 15 15 11 15 13 16 16 17 16 12 12 12 9 17 30 17 8 12 18 42 21 9 15 19 32 28 14 11 20 38 31 14 16

21 25 20 11 15 22 17 11 10 18 23 10 16 13 24 24 12 8 12 21 25 8 8 9 16 26 9 15 15 18 27 15 11 12 18 28 12 17 15 20 29 5 9 14 15 30 9 16 15 16 31 18 14 12 12 32 12 18 8 8 33 16 9 8 8 34 15 15 10 18 35 17 16 15 36 8 8 16 37 12 10 17 38 10 11 11 39 7 13 12 40 Sum 560 553 448 536

Figure 1 shows a comparison of the number of mortality by each type, showing a much lower number of deaths in the administration of the chitovita solution, the composition of the present invention.

Comparison Table A (chitonal acid) B (from Chitovita) C (quitovitav) D (Vita Chorus) Harvest 11,500 10,800 10,000 9,100 Shipment Count 10,940 10,247 9,552 8,564 Shipment date 40 40 35 40 Shipping rate () 95.1 94.9 95.5 94.1 Total feed consumption (kg) 33,384 31,916 26,950 26,744 Total Shipping Weight (kg) 18,117 16,887 14,950 14,002 Feed rate 1.84 1.89 1.80 1.91 Shipment Average Weight (kg) 1.656 1.648 1.565 1.635 Production index 214 207 237 201 Drug Cost (KRW) 297,000 (9,000 per kg) 248,000 (4,000 per kg) 364,000 (7,000 per L) 468,000 (9,000 per L) Maridan sales costs (won) 1,159 1,154 1,096 1,144 Net profit per head (KRW) -329 -356 -325 -377 Net profit (based on 10,000 numbers) -3,290,000 -3,560,000 -3,250,000 -3,770,000 Comparative profit (based on 10,000 numbers) 480,000 210,000 520,000

Since feed efficiency and feed demand rate do not consider the number of deaths from harvest to shipment, various production index formulas are presented to grasp overall farm management status because it is insufficient in actual farm management analysis. In the table above

Shipment Rate () = Shipments / Harvest × 100,

Feed demand ratio = total feed consumption / total weight

Average shipment weight = total shipment weight / number of shipments,

Production index (P.I) = (shipment rate × shipping average weight) / (shipment age x feed demand rate) × 100

It is calculated using the formula, and the results shown in the table

Shipment rate (): Chitovita liquid (95.5)> Chitonal acid (95.1)> Chitovita acid (94.9)> Vita chorus (94.1)

Feed demand rate: chitobita solution (1.80) ＞ chitonal acid (1.84) ＞ chitobita acid (1.89) ＞ vitachorus (1.91)

Shipment Average Weight: Chitonal Acid (1.656) ＞ Chitovitasan (1.648) ＞ Vita Chorus (1.635) ＞ Kitobita Liquid (1.565)

Production Index: Chitovita liquid (237) ＞ chitonal acid (214) ＞ chitovita acid (207) ＞ vita chorus (201)

Is in order.

First, chitonal acid and chitovitasan were modern cages, and chitovita fluid and vitachorus were conventional cages, which made it difficult to measure ammonia gas, but chitonal cages had less odor than chitovitasan cages. Tobita was less smelly than Vitachorus. More than 30 deaths per day occurred in A and B dongs from 17 to 21 days of age, and all of the company commissioned the veterinary science quarantine to determine the mortality and odor. The Veterinary Research and Quarantine Service decided that it would not be economical anymore, but the results of A, B, and C were better than D, which was not infected with E. coli.

When comparing the productivity, chitovita solution was the best in the overall productivity index, followed by chitonal acid, chitovita acid, and vitachorus. Veterinary Quarantine Service analyzed ND antibody titer and found 0 (7), 1 (3), and 5 (1) in A, B, and D dongs negatively vaccinated with ND + IBD at 8 days of age. It showed little effect of the vaccine. On the other hand, C-dong, which was negatively vaccinated with ND + IBD at 13 days of age, had a relatively high titer of 2 (2), but a second vaccine was needed to obtain at least 3 to prevent mortality in broilers. The biggest problem with the farm was the lack of ventilation. There was an exhaust fan, but the intake facility was insufficient, so the exhaust fan itself could not operate properly. If you had adequate ventilation, you would have been able to achieve better results.

As can be seen from the above embodiment, the composition according to the present invention shows a significant improvement in the weight gain and feed efficiency in contrast to the conventional feed and negative additives, so the conventional feed and negative additives Alternatively, it can be usefully used.

In other words, by adding chitosan oligosaccharides and taurine, in addition to the general vitamins used as feed and negative nutritional supplements, not only the useful effects of vitamins, but also the antimicrobial, immunopotentiating, and antihypertensive effects of chitosan oligosaccharides are known to regulate osmotic pressure and antioxidant activity of taurine. By synergistically acting synergistically with the action, detoxification, membrane stabilization, neuromodulation, optic nerve protection, cardiac and neuronal development, animal growth, feed efficiency, weight gain, immunity, anti-competence, etc. Excellent effect.

Claims (3)

Feed containing chitosan oligosaccharides and taurine, containing 3 to 60 g / L of chitosan oligosaccharides and 10 to 300 g / L of aminoethyl sulfonic acid as main components, and mixed and administered 1 to 4 liters per ton of animal feed and negative Negative nutritional composition. According to claim 1, wherein the composition is vitamins, amino acids and minerals necessary for the animal chitosan oligosaccharides and taurine-containing feed and negative nutritional composition further comprises. The method of claim 1 or 2, wherein the vitamin is vitamin A, vitamin D ₃ , vitamin E, vitamin B ₁ , vitamin B ₂ , vitamin B ₆ , vitamin B ₁₂ , vitamin C, vitamin K ₃ , nicotinic acid amide, folic acid Chitosan oligosaccharides and taurine-containing feed and negative nutritional composition comprising calcium pantothenate and choline chloride.