CN101524366B - Method for preparing compound biological activity sterilizing fluid - Google Patents
Method for preparing compound biological activity sterilizing fluid Download PDFInfo
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- CN101524366B CN101524366B CN2009100198793A CN200910019879A CN101524366B CN 101524366 B CN101524366 B CN 101524366B CN 2009100198793 A CN2009100198793 A CN 2009100198793A CN 200910019879 A CN200910019879 A CN 200910019879A CN 101524366 B CN101524366 B CN 101524366B
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- CN
- China
- Prior art keywords
- low molecular
- iodine
- fluid
- biological activity
- chitosan
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- Expired - Fee Related
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- 239000012530 fluid Substances 0.000 title claims abstract description 15
- 230000001954 sterilising effect Effects 0.000 title claims abstract description 14
- 230000004071 biological effect Effects 0.000 title claims abstract description 11
- 150000001875 compounds Chemical class 0.000 title claims abstract description 11
- 238000000034 method Methods 0.000 title abstract 2
- 229920001661 Chitosan Polymers 0.000 claims abstract description 35
- CPKVUHPKYQGHMW-UHFFFAOYSA-N 1-ethenylpyrrolidin-2-one;molecular iodine Chemical compound II.C=CN1CCCC1=O CPKVUHPKYQGHMW-UHFFFAOYSA-N 0.000 claims abstract description 17
- 229920000153 Povidone-iodine Polymers 0.000 claims abstract description 17
- 229960001621 povidone-iodine Drugs 0.000 claims abstract description 17
- 238000003756 stirring Methods 0.000 claims abstract description 6
- 239000004094 surface-active agent Substances 0.000 claims abstract description 5
- 239000007788 liquid Substances 0.000 claims description 20
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 claims description 11
- 239000000243 solution Substances 0.000 claims description 10
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 6
- 238000002360 preparation method Methods 0.000 claims description 6
- 239000000203 mixture Substances 0.000 claims description 5
- 230000008961 swelling Effects 0.000 claims description 5
- 239000012085 test solution Substances 0.000 claims description 4
- GSEJCLTVZPLZKY-UHFFFAOYSA-N Triethanolamine Chemical compound OCCN(CCO)CCO GSEJCLTVZPLZKY-UHFFFAOYSA-N 0.000 claims description 3
- 125000000217 alkyl group Chemical group 0.000 claims description 3
- 125000002091 cationic group Chemical group 0.000 claims description 2
- 229910052740 iodine Inorganic materials 0.000 abstract description 11
- 239000011630 iodine Substances 0.000 abstract description 11
- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 abstract description 10
- 229920000036 polyvinylpyrrolidone Polymers 0.000 abstract description 6
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 abstract description 6
- PNDPGZBMCMUPRI-UHFFFAOYSA-N iodine Chemical compound II PNDPGZBMCMUPRI-UHFFFAOYSA-N 0.000 abstract description 2
- 231100000053 low toxicity Toxicity 0.000 abstract description 2
- 230000001105 regulatory effect Effects 0.000 abstract description 2
- 241000894006 Bacteria Species 0.000 abstract 2
- 230000002401 inhibitory effect Effects 0.000 abstract 2
- WHNWPMSKXPGLAX-UHFFFAOYSA-N N-Vinyl-2-pyrrolidone Chemical compound C=CN1CCCC1=O WHNWPMSKXPGLAX-UHFFFAOYSA-N 0.000 abstract 1
- 239000000969 carrier Substances 0.000 abstract 1
- 239000003153 chemical reaction reagent Substances 0.000 abstract 1
- 230000007794 irritation Effects 0.000 abstract 1
- 210000004877 mucosa Anatomy 0.000 abstract 1
- 229940069328 povidone Drugs 0.000 abstract 1
- 150000003242 quaternary ammonium salts Chemical class 0.000 abstract 1
- 230000000844 anti-bacterial effect Effects 0.000 description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
- 238000004659 sterilization and disinfection Methods 0.000 description 5
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 4
- 239000001267 polyvinylpyrrolidone Substances 0.000 description 4
- 230000001681 protective effect Effects 0.000 description 4
- SNRUBQQJIBEYMU-UHFFFAOYSA-N Dodecane Natural products CCCCCCCCCCCC SNRUBQQJIBEYMU-UHFFFAOYSA-N 0.000 description 3
- 125000003438 dodecyl group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])* 0.000 description 3
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 2
- 229960000583 acetic acid Drugs 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- -1 cationic polysaccharide Chemical class 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 210000002421 cell wall Anatomy 0.000 description 2
- 230000001684 chronic effect Effects 0.000 description 2
- 239000000645 desinfectant Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000012362 glacial acetic acid Substances 0.000 description 2
- 239000000693 micelle Substances 0.000 description 2
- 231100000957 no side effect Toxicity 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- 229920001282 polysaccharide Polymers 0.000 description 2
- 239000005017 polysaccharide Substances 0.000 description 2
- 229910052708 sodium Inorganic materials 0.000 description 2
- 239000011734 sodium Substances 0.000 description 2
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 239000002085 irritant Substances 0.000 description 1
- 231100000021 irritant Toxicity 0.000 description 1
- 239000003446 ligand Substances 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 238000004375 physisorption Methods 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Landscapes
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention discloses a method for preparing compound biological activity sterilizing fluid, which comprises the steps: a proper amount of surface active agent is added to low molecular chitosan quaternary ammonium salt or low molecular chitosan solution under a stirring condition to be used as first fluid, povidone iodine is swelled to be used as second fluid, and the first fluid and the second fluid are evenly mixed according to a proper proportion and are regulated to a prescriptive range by a chemical reagent to obtain the compound biological activity sterilizing fluid. The prepared compound biological activity sterilizing fluid uses low molecular chitosan and povidone as double carriers, the chitosan not only can strengthen the bacterium inhibiting and sterilizing effects of the povidone iodine, but also has a certain adsorbing function for iodine released by the povidone iodine, causes the release of the iodine to be doubly controlled to reduce the volatilization of the iodine and increase the release uniformity of the iodine, the chitosan has degradability, the skin mucosa protecting function and the bacterium inhibiting function, therefore, the compound biological activity sterilizing fluid is stable and environmental-friendly and has high efficiency, low toxicity, low irritation and the skin protecting function.
Description
Technical field
The present invention relates to a kind of disinfectant, particularly relate to a kind of preparation method of compound biological activity sterilizing fluid.
Background technology
Povidone iodine is the complex of iodine and polyvinylpyrrolidone (PVP), because it has film forming, bonding, detoxifcation, chronic release, water solublity is strong and advantage (" Chinese pharmacopoeia version in 2005 such as broad-spectrum bactericidal action, clinical application notice .2001.911), povidone iodine is mucocutaneous by being applied to more and more widely, sterilization, the disinfection of operation and apparatus etc.But this class is that product original micelle power meeting after diluting of carrier is destroyed with the surfactant, makes the stability decreases of active iodine, and free-iodine concentration increases, and not only influences its disinfection effect, and has increased Mucocutaneous zest.Chitosan is the natural cationic polysaccharide of a class that nature extensively exists; has excellent biological compatibility; and non-stimulated having no side effect, low molecular n-trimethyl chitosan chloride or low molecular chitosan all have antibacterial action preferably, and have a good protective action to mucocutaneous.There is report to think that chitosan gathers at the cell wall place easily as a kind of natural polycation, stops nutrient substance to enter cell, make cell failure death by cell wall; Low molecular chitosan then can hinder DNA to the transcribing of RNA, and influences the growth and breeding of antibacterial, thereby produces antibacterial action.
Summary of the invention
The technical problem to be solved in the present invention provides a kind of preparation method of compound biological activity sterilizing fluid, and this disinfectant solution has good protective action to mucocutaneous, and little to Mucocutaneous zest.
For solving the problems of the technologies described above, the preparation method of this compound biological activity sterilizing fluid is that low molecular n-trimethyl chitosan chloride or low molecular chitosan solution are added cationic surface active agent 1%-10% as 1 liquid under stirring condition, with the povidone iodine swelling as 2 liquid, 1 liquid and 2 liquid are pressed the proper proportion mix homogeneously, adjust pH value to 2-7 with sodium hydroxide test solution or triethanolamine solution.
Described low molecular n-trimethyl chitosan chloride or low molecular chitosan solution have following general formula, and its weight average molecular weight≤100,000
R wherein
1, R
2Be alkyl, R
1General formula is C
nH
2n, R
2General formula is C
nH
2n+1, n=1,2,3,4,5.
Described povidone iodine has following general formula, and presses dry product and calculate, and contains available iodine and should be 9.0%-12.0%.
The mass percentage concentration of described low molecular n-trimethyl chitosan chloride or low molecular chitosan solution is 0.01-4%; The mass percentage concentration of povidone iodine is 0.5-10%.
The present invention has following advantage:
1, chitosan is the natural cationic polysaccharide of a class that nature extensively exists; has excellent biological compatibility; and non-stimulated having no side effect, low molecular n-trimethyl chitosan chloride or low molecular chitosan all have antibacterial action preferably, and have a good protective action to mucocutaneous.
2, povidone iodine is the complex of iodine and polyvinylpyrrolidone (PVP) since it have film forming, bonding, detoxifcation, chronic release, water solublity is strong and advantage such as broad-spectrum bactericidal action is mucocutaneous by being applied to more and more widely, sterilization, the disinfection of operation and apparatus etc.The product that the present invention makes is a double carrier with low molecular chitosan and polyvidone; chitosan not only can strengthen antibacterial, the bactericidal effect of povidone iodine; and the iodine that povidone iodine is discharged has certain adsorption; make the release of iodine obtain dual control; reduced the volatilization of iodine; improved the release uniformity of iodine; reduced Mucocutaneous zest; and chitosan itself has degradability, mucocutaneous protective effect and bacteriostasis, thus make that this product is more stable, efficient than like product, safety and low toxicity, low irritant.
3, add proper amount of surfactant, can improve the ligand complex effect of low molecular n-trimethyl chitosan chloride or low molecular chitosan and free-iodine, and itself have micelle power and physisorption, the stability of this product is further improved.
4, regulating pH value to prescribed limit is in order to reduce product to Mucocutaneous zest.
The specific embodiment
Below in conjunction with specific embodiment the present invention is described in further detail:
Embodiment 1
Getting the 0.5g low molecular chitosan, to be dissolved in 60ml concentration be in 0.2% the glacial acetic acid aqueous solution, under stirring condition, add the two dodecyl bi-quaternary ammonium salts of 5g, as 1 liquid, with the 2g povidone iodine in 35ml water swelling as 2 liquid, with 1 liquid and 2 liquid mix homogeneously, hydro-oxidation sodium test solution is transferred pH value to 4.5 promptly.
Embodiment 2
Getting the 1.0g low molecular chitosan, to be dissolved in 70ml concentration be in 0.2% the glacial acetic acid aqueous solution, under stirring condition, add the two dodecyl bi-quaternary ammonium salts of 4g, as 1 liquid, with the 1g povidone iodine in 25ml water swelling as 2 liquid, with 1 liquid and 2 liquid mix homogeneously, hydro-oxidation sodium test solution is transferred pH value to 3.1 promptly.
Embodiment 3
Getting the 1.5g low molecular n-trimethyl chitosan chloride is dissolved in the 70ml water, under stirring condition, add the two dodecyl bi-quaternary ammonium salts of 3.5g, as 1 liquid, with the 0.5g povidone iodine in 25ml water swelling as 2 liquid, with 1 liquid and 2 liquid mix homogeneously, add triethanolamine and transfer pH value to 6.0 promptly.
Low molecular n-trimethyl chitosan chloride of the present invention or low molecular chitosan solution have following general formula:
R wherein
1, R
2Be alkyl, R
1General formula is C
nH
2n(n=1,2,3,4,5 ...), R
2General formula is C
nH
2n+1(n=1,2,3,4,5 ...).
Povidone iodine of the present invention has following general formula:
Claims (2)
1. the preparation method of a compound biological activity sterilizing fluid, it is characterized in that low molecular n-trimethyl chitosan chloride or low molecular chitosan solution are added cationic surface active agent 1%-10% as 1 liquid under stirring condition, with the povidone iodine swelling as 2 liquid, 1 liquid and 2 liquid are pressed the proper proportion mix homogeneously, adjust pH value to 2-7 with sodium hydroxide test solution or triethanolamine solution, described low molecular n-trimethyl chitosan chloride or low molecular chitosan solution have following general formula, and its weight average molecular weight≤100,000
R wherein
1, R
2Be alkyl, R
1General formula is C
nH
2n, R
2General formula is C
nH
2n+1, n=1,2,3,4,5.
2. the preparation method of compound biological activity sterilizing fluid according to claim 1, the mass percentage concentration that it is characterized in that low molecular n-trimethyl chitosan chloride or low molecular chitosan solution is 0.01-4%; The mass percentage concentration of povidone iodine is 0.5-10%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009100198793A CN101524366B (en) | 2009-03-21 | 2009-03-21 | Method for preparing compound biological activity sterilizing fluid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009100198793A CN101524366B (en) | 2009-03-21 | 2009-03-21 | Method for preparing compound biological activity sterilizing fluid |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101524366A CN101524366A (en) | 2009-09-09 |
| CN101524366B true CN101524366B (en) | 2011-06-08 |
Family
ID=41092541
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2009100198793A Expired - Fee Related CN101524366B (en) | 2009-03-21 | 2009-03-21 | Method for preparing compound biological activity sterilizing fluid |
Country Status (1)
| Country | Link |
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| CN (1) | CN101524366B (en) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102108033B (en) * | 2009-12-25 | 2013-03-06 | 东莞市施普旺生物科技有限公司 | Chitin iodine solution |
| CN103720711A (en) * | 2012-10-10 | 2014-04-16 | 上海工程技术大学 | Method for preparing amphiphilic chitosan group nano iodine disinfectant |
| CN104434994A (en) * | 2014-11-28 | 2015-03-25 | 成都顺发消洗科技有限公司 | Mucocutaneous disinfectant |
| CN104434995A (en) * | 2014-11-28 | 2015-03-25 | 成都顺发消洗科技有限公司 | Preparation method of mucocutaneous disinfectant |
| CN104644667A (en) * | 2015-02-12 | 2015-05-27 | 天津大学 | Compound disinfectant and preparation method thereof |
| CN105557751B (en) * | 2015-05-18 | 2018-08-07 | 深圳市安多福动物药业有限公司 | High-molecular biologic organic iodine and preparation method thereof, aquaculture disease prevention and cure method |
| CN105560271B (en) * | 2016-03-04 | 2019-06-07 | 深圳市阳光百度生物科技发展有限公司 | Chitosan Betagen Solution and its manufacture craft |
| CN110037050B (en) * | 2019-05-08 | 2021-02-02 | 山西大学 | High-efficiency compound iodine solution and preparation method thereof |
| CN110237216A (en) * | 2019-07-03 | 2019-09-17 | 牡丹江医学院附属红旗医院 | A kind of skin antiseptic and preparation method and application |
| KR102555251B1 (en) * | 2020-12-04 | 2023-07-12 | 단국대학교 천안캠퍼스 산학협력단 | Hemostasis composition comprising the novel composition |
| CN113144281B (en) * | 2021-04-19 | 2022-04-15 | 深圳市安多福消毒高科技股份有限公司 | Wound surface disinfection gel and preparation method thereof |
-
2009
- 2009-03-21 CN CN2009100198793A patent/CN101524366B/en not_active Expired - Fee Related
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| Publication number | Publication date |
|---|---|
| CN101524366A (en) | 2009-09-09 |
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Effective date of registration: 20120612 Address after: 261061, Shandong, Weifang province hi tech Zone, east of health, South, high tech two Road East (bio pharmaceutical science and Technology Industrial Park) Patentee after: SHANDONG BEINUO MEDICAL BIOLOGY TECHNOLOGY CO., LTD. Address before: Weifang City, Shandong province 261041 Dongfeng East Street Kuiwei District No. 360 Taihua business building A block 1209 Patentee before: Liu Weifeng |
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Denomination of invention: Method for preparing compound biological activity sterilizing fluid Effective date of registration: 20160831 Granted publication date: 20110608 Pledgee: Postal Savings Bank of China, Limited by Share Ltd, Weifang branch Pledgor: SHANDONG BEINUO MEDICAL BIOLOGY TECHNOLOGY CO., LTD. Registration number: 2016980000141 |
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| CF01 | Termination of patent right due to non-payment of annual fee |