CN101491225B - Rearing method capable of increasing cuttlebone medical value of Sepiella maindroni - Google Patents
Rearing method capable of increasing cuttlebone medical value of Sepiella maindroni Download PDFInfo
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- CN101491225B CN101491225B CN2009100963075A CN200910096307A CN101491225B CN 101491225 B CN101491225 B CN 101491225B CN 2009100963075 A CN2009100963075 A CN 2009100963075A CN 200910096307 A CN200910096307 A CN 200910096307A CN 101491225 B CN101491225 B CN 101491225B
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- cuttlebone
- amino acid
- sepiella maindroni
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- culture
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
Abstract
The invention discloses a culture method capable of improving the cuttlebone medicinal value of sepiella maindroni. The method comprises the following steps: temporary parent culture, seedling raising, larva culture and adult culture, wherein adding the step of screening the amino acid component of the parent mantle for the temporary cultured sepiella maindroni in the step of temporary parent culture in the culture process so as to select the parent sepiella maindroni with the cuttlebone rapid proliferation potential; in the step of larva culture, feeding the sepiella maindroni larva with the artemia or copepoda which is strengthened by the amino acid relevant with cuttlebone proliferation; and in the step of adult culture, feeding the sepiella maindroni adult with the fodder which contains the amino acid relevant with cuttlebone proliferation. By supplying the relevant ammonia acid greatly needed by the cuttlebone growth, the method can ensure the nutritional need for the endoskeleton growth of the sepiella maindroni, in particular the synthesis need for the biomineralization regulatory protein. The method can use up the aspartic acid in the sepiella maindroni to promote the mineralization. The method has the advantages of simple steps, remarkable effects, and suitability for large-scale generalization.
Description
Technical field
The present invention relates to the breeding method of a kind of aquatic livestock, particularly improve the breeding method of the cuttlebone medical value of Sepiella maindroni.
Background technology
Cuttlefish is commonly called as inkfish, belongs to Cephalopoda, Decapoda.Cuttlefish meat contains several amino acids, and is nutritious, the meat flavour deliciousness.Cuttle bone claims cuttlebone, has effects such as convergence, relieving haperacidity, hemostasis, once is one of four big fishery objects of China, and is stronger to the adaptability of temperature, very wide in the coastal distribution of China, is the very high traditional fishery kind of a kind of economic worth.Because overfishing and ecological degeneration since the seventies in 20th century, decay successively occurred.Obtain a large amount of wild cuttlefishes with crossing the approach of fishing for, thus the mode that obtains cuttlebone inefficiency but also destroy ecological not only.Be badly in need of the feasible method of a kind of reality, in conjunction with modern biochemical technology, produce the method for this medicinal material of cuttlebone in a large number by industrial aquaculture.
Summary of the invention
The breeding method that the purpose of this invention is to provide the cuttlebone medical value that improves Sepiella maindroni improve growth rate and its medical value of Sepiella maindroni cuttlebone with this, and this method step is simple, effect is remarkable.
The present invention solves the problems of the technologies described above the technical scheme that is adopted: the breeding method that improves the cuttlebone medical value of Sepiella maindroni, comprise that parent is supported temporarily, grown seedlings, larya culturing and adult culture step, wherein: the parent in cultivating process is supported temporarily increases the Sepiella maindroni that pallial amino acid composition screening is supported temporarily to parent in the step, to choose the parent Sepiella maindroni with the quick hyperplasia potentiality of cuttlebone; Use the halogen worm or the copepoda breeding Sepiella maindroni young of strengthening through cuttlebone hyperplasia related amino acid in the larya culturing step; Culture step at adult and use the mixed bait breeding Sepiella maindroni adult that contains cuttlebone hyperplasia related amino acid.Adopt the above operation can be by replenishing a large amount of required related amino acids of cuttlebone growth in good time,, and promote its mineralization based on this indirectly with the nutritional need that guarantees the growth of the cuttlefish endoskeleton synthetic needs of biomineralization adjusting albumen particularly.A large amount of cuttlebone hyperplasia related amino acids can exhaust the aspartic acid in the squid body, thereby reducing the calcium binding partner is the protein of carboxyl aspartic acid, improving the calcium binding site indirectly then is the glycoprotein and the phosphoprotein of sulfuric acid or phosphate group, the latter combines with calcium with low-affinity, and participate in trace element in endoskeletal deposition, improve the medical value of cuttlebone.
For optimizing technique scheme, the technical measures of taking also comprise:
Above-mentioned cuttlebone hyperplasia related amino acid is glycine, serine and alanine.
The amino acid composition screening comprises the steps: to increase by 2 times to the 10 times raising parent Sepiella maindronis that glycine or serine or alanine content are normal feeding volume in feed, continues 2 days to 5 days; Then, get the mantle tissue of parent Sepiella maindroni, detect amino acid content and account for total amino acid content and be used to grow seedlings less than 7% parent to choose glycine.The index that detects amino acid content comprises that also serine accounts for total amino acid content less than 5%.
The index that detects amino acid content comprises that also alanine accounts for total amino acid content less than 6%.
Adopt throw something and feed cuttlefish and detect amino acid content of high cuttlebone hyperplasia related amino acid amount, can sieve the individuality not strong to the amino acid regulating power, thereby filter out parent Sepiella maindroni individuality, to improve the efficient of technical solution of the present invention with the quick hyperplasia potentiality of cuttlebone.
In the larya culturing step, the step of strengthening the halogen worm comprises the chlorella nursing halogen worm of the sea water immersion of use through containing cuttlebone hyperplasia related amino acid; In the larya culturing step, the step of strengthening copepoda comprises the nauplius of the yeast nursing copepoda that the seawater of use through containing cuttlebone hyperplasia related amino acid cultivated.Resulting filial generation cuttlefish after filtering out parent Sepiella maindroni with the quick hyperplasia potentiality of cuttlebone and since heredity the characteristic of parent, the overwhelming majority has the quick hyperplasia potentiality of cuttlebone equally.
Culture in the step at adult, the mixed bait that uses is: contain the manufactured feed of basestocks 50kg, white fish meal 28kg, microcrystalline cellulose 3.6kg, dextrin 14kg, biological extraction oil 3.8kg, and manufactured feed also is added with glycine 70g, serine 50g and alanine 60g.Raise the Main Stage that adult is cuttlebone growth and the accumulation of medicinal part with the feed that contains cuttlebone hyperplasia related amino acid, cuttlebone is as endoskeleton, its growth is the process of the outside accumulation of calcium successively in having, adopting the purpose of special feed in this stage is to impel calcium to deposit in a large number, increases the medicinal portion of cuttlebone as far as possible.
Compared with prior art, the invention has the advantages that and adopt above operation can pass through to replenish a large amount of required related amino acids of cuttlebone growth in good time, with the nutritional need that guarantees the growth of cuttlefish endoskeleton particularly biomineralization regulate the synthetic needs of albumen, and promote its mineralization indirectly by the aspartic acid that exhausts in the squid body.Adopt throw something and feed cuttlefish and detect amino acid content of high cuttlebone hyperplasia related amino acid amount, filter out parent Sepiella maindroni individuality, and make filial generation have same potentiality accordingly to improve the efficient of technical solution of the present invention with the quick hyperplasia potentiality of cuttlebone.Adopt the inventive method can improve growth rate and its medical value of Sepiella maindroni cuttlebone, and this method step is simple, effect is remarkable, is suitable for large-scale promotion.
Embodiment
Below in conjunction with attached embodiment the present invention is described in further detail.
Embodiment: the breeding method that improves the cuttlebone medical value of Sepiella maindroni, comprise that parent is supported temporarily, grown seedlings, larya culturing and adult culture step, wherein: the parent in cultivating process is supported temporarily increases the Sepiella maindroni that pallial amino acid composition screening is supported temporarily to parent in the step, to choose the parent Sepiella maindroni with the quick hyperplasia potentiality of cuttlebone; Use the halogen worm or the copepoda breeding Sepiella maindroni young of strengthening through cuttlebone hyperplasia related amino acid in the larya culturing step; Culture step at adult and use the mixed bait breeding Sepiella maindroni adult that contains cuttlebone hyperplasia related amino acid.
Cuttlebone hyperplasia related amino acid is glycine, serine and alanine.The amino acid composition screening comprises the steps: to increase by 3 times of raising parent Sepiella maindronis that glycine or serine or alanine content are normal feeding volume in feed, continues 5 days; Then, get the mantle tissue of parent Sepiella maindroni, detect amino acid content and account for total amino acid content and be used to grow seedlings less than 6.1% parent to choose glycine.
The index that detects amino acid content comprises that also serine accounts for total amino acid content less than 4.4%.
The index that detects amino acid content comprises that also alanine accounts for total amino acid content less than 5.6%.
In the larya culturing step, the step of strengthening the halogen worm comprises the chlorella nursing halogen worm of the sea water immersion of use through containing cuttlebone hyperplasia related amino acid; In the larya culturing step, the step of strengthening copepoda comprises the nauplius of the yeast nursing copepoda that the seawater of use through containing cuttlebone hyperplasia related amino acid cultivated.
Culture in the step at adult, the mixed bait that uses is: the manufactured feed that contains basestocks 50kg, white fish meal 28kg, microcrystalline cellulose 3.6kg, dextrin 14kg, biological extraction oil 3.8kg, and manufactured feed also is added with glycine 70g, serine 50g and alanine 60g, this prescription is as aforesaid normal feeding volume radix, and the amount that increases cuttlebone hyperplasia related amino acid again on the basis of this proportioning is to be used for screening.
Although described the present invention in conjunction with preferred embodiment; right its is not in order to limit the present invention; any those skilled in the art; under the situation that does not break away from the spirit and scope of the present invention; can implement the displacement and the modification of various changes, coordinate to the theme of listing here, so protection scope of the present invention is as the criterion when looking the claim restricted portion that is proposed.
Claims (4)
1. improve the breeding method of the cuttlebone medical value of Sepiella maindroni, comprise that parent is supported temporarily, grown seedlings, larya culturing and adult culture step, it is characterized in that: the parent in cultivating process is supported temporarily increases the Sepiella maindroni that pallial amino acid composition screening is supported temporarily to parent in the step, to choose the parent Sepiella maindroni with the quick hyperplasia potentiality of cuttlebone; Use the halogen worm or the copepoda breeding Sepiella maindroni young of strengthening through cuttlebone hyperplasia related amino acid in the larya culturing step; Culture step at adult and use the mixed bait breeding Sepiella maindroni adult that contains cuttlebone hyperplasia related amino acid; Described cuttlebone hyperplasia related amino acid is glycine, serine and alanine;
Culture in the step at adult, the described mixed bait that uses is: contain the manufactured feed of basestocks 50kg, white fish meal 28kg, microcrystalline cellulose 3.6kg, dextrin 14kg, biological extraction oil 3.8kg, and described manufactured feed also is added with glycine 70g, serine 50g and alanine 60g; This prescription is as normal feeding volume radix, and the amount that increases cuttlebone hyperplasia related amino acid on the basis of this proportioning is to be used for screening;
Described amino acid composition screening comprises the steps: to increase by 2 times to the 10 times described parent Sepiella maindronis of raising that glycine or serine or alanine content are normal feeding volume in feed, continues 2 days to 5 days; Then, get the mantle tissue of described parent Sepiella maindroni, detect amino acid content and account for total amino acid content and be used to grow seedlings less than 7% parent to choose glycine.
2. the breeding method of the cuttlebone medical value of raising Sepiella maindroni according to claim 1 is characterized in that: the index that detects amino acid content comprises that also serine accounts for total amino acid content less than 5%.
3. the breeding method of the cuttlebone medical value of raising Sepiella maindroni according to claim 2 is characterized in that: the index that detects amino acid content comprises that also alanine accounts for total amino acid content less than 6%.
4. the breeding method of the cuttlebone medical value of raising Sepiella maindroni according to claim 3, it is characterized in that: in the larya culturing step, the step of strengthening the halogen worm comprises the chlorella nursing halogen worm of the sea water immersion of use through containing cuttlebone hyperplasia related amino acid; In the larya culturing step, the step of strengthening copepoda comprises the nauplius of the yeast nursing copepoda that the seawater of use through containing cuttlebone hyperplasia related amino acid cultivated.
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| CN2009100963075A CN101491225B (en) | 2009-02-19 | 2009-02-19 | Rearing method capable of increasing cuttlebone medical value of Sepiella maindroni |
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| CN2009100963075A CN101491225B (en) | 2009-02-19 | 2009-02-19 | Rearing method capable of increasing cuttlebone medical value of Sepiella maindroni |
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| CN101491225A CN101491225A (en) | 2009-07-29 |
| CN101491225B true CN101491225B (en) | 2011-07-20 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106900600A (en) * | 2016-12-30 | 2017-06-30 | 浙江海洋大学 | A kind of cultivation method for enhancing for improving Sepiella maindroni reproductive capacity |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102934620B (en) * | 2012-11-21 | 2014-07-16 | 浙江海洋学院 | Method for feeding sepiella maindroni |
| CN105533267A (en) * | 2015-12-17 | 2016-05-04 | 宁波大学 | Special phagostimulant for sepia pharaonis and application of special phagostimulant |
| CN107372272B (en) * | 2017-07-21 | 2020-10-13 | 浙江海洋大学 | Long-acting physical sign device of cuttlefish based on endoskeleton cuttlebone |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101243836A (en) * | 2008-03-07 | 2008-08-20 | 宁波大学 | Method for nursing sepiella maindroni with soft compound feed |
| CN101243837A (en) * | 2008-03-07 | 2008-08-20 | 宁波大学 | Compound feed for breeding Sepiella maindroni |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101243836A (en) * | 2008-03-07 | 2008-08-20 | 宁波大学 | Method for nursing sepiella maindroni with soft compound feed |
| CN101243837A (en) * | 2008-03-07 | 2008-08-20 | 宁波大学 | Compound feed for breeding Sepiella maindroni |
Non-Patent Citations (3)
| Title |
|---|
| 李正等.饵料对曼氏无针乌贼幼体生长、成活率及营养成分的影响.《大连水产学院学报》.2007,第22卷(第6期),436-441. * |
| 樊晓旭等.投喂四种饵料对曼氏无针乌贼(Sepiellamaindroni)繁殖性能的影响.《海洋与湖沼》.2008,第39卷(第6期),634-642. * |
| 符方尧等.曼氏无针乌贼的药用价值及其人工养殖.《中国水产》.2005,(第12期),26-27. * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106900600A (en) * | 2016-12-30 | 2017-06-30 | 浙江海洋大学 | A kind of cultivation method for enhancing for improving Sepiella maindroni reproductive capacity |
| CN106900600B (en) * | 2016-12-30 | 2020-11-13 | 浙江海洋大学 | Enhanced cultivation method for improving fertility of sepiella maindroni |
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| CN101491225A (en) | 2009-07-29 |
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